A production method for extracting high-purity mogroside V from momordica grosvenori

Abstract

This application relates to the field of monk fruit extraction technology, and in particular to a production method for extracting high-purity mogroside V from monk fruit, comprising the following steps: Step 1: pretreatment and enzyme inactivation of fresh fruit; Step 2: multiple pressing, collecting the juice after each pressing and combining it to obtain fruit juice; Step 3: countercurrent extraction of the fruit pomace with a compound enzyme-assisted extraction solvent of a 0.1-0.3% concentration of compound enzyme aqueous solution; Step 4: treatment with a compound clarifying agent, collecting the centrifuged liquid; Step 5: purification using a simulated moving bed, concentrating to obtain monk fruit concentrated juice product; Step 6: concentration and desalination using a nanofiltration membrane, concentrating until the solid content reaches 30-40%; Step 7: spray drying and finished product testing, packaging and warehousing after all indicators meet the food additive standards. This application solves the problem of low efficiency caused by frequent manual shutdowns and production interruptions at a single workstation in the prior art.

Description

Technical Field

[0001] This application relates to the field of monk fruit extraction technology, and in particular to a production method for extracting high-purity mogroside V from monk fruit. Background Technology

[0002] Currently, in the process of extracting high-purity mogroside V from monk fruit, pressing the fresh monk fruit is a key initial step. The purpose is to separate the juice from the fruit through physical compression, providing a basic raw material for subsequent purification steps. At present, various pressing devices are commonly used in the industry to complete this operation. Among them, the low-temperature pressing device for processing camellia seeds disclosed in Chinese patent announcement number "CN214991395U" has been tried by some companies in the pressing process of monk fruit because it has certain low-temperature pressing functions. The device is mainly composed of a fixed base plate, side plate, linkage plate, linkage component, pressing hammer, pressing barrel and other structures. The first lifting plate and the second lifting plate are moved by the second motor. The pressing barrel is separated from the pressing filter screen by the limiting block, so that the residue after pressing is left on the pressing filter screen.

[0003] However, when such devices are applied to large-scale monk fruit extraction, their shortcomings and deficiencies become increasingly apparent. Firstly, the operation is cumbersome and lacks continuity. After each pressing, the monk fruit residue remains on the filter screen inside the pressing barrel. To proceed with the next pressing, the machine must be manually stopped and the residue removed from the barrel before new monk fruit can be added. In large-scale production, where continuous pressing of large quantities of monk fruit is required, this reliance on manual residue removal severely disrupts the production process, significantly increasing operating time and labor costs. Secondly, the lack of automated tilting and cleaning mechanisms results in low cleaning efficiency. Because the pressing barrel lacks a tilting function and is not equipped with corresponding cleaning components, the juice and fine residue remaining on the inner wall of the pressing barrel, the pressing hammers, and the filter screen after each pressing is difficult to remove quickly. Furthermore, monk fruit juice contains a large amount of sugar and colloidal substances, which, if not cleaned promptly, will further exacerbate the problem. The existing pressing device is prone to microbial growth or oxidation, affecting the purity of the raw materials for subsequent pressing. Therefore, a significant amount of time must be spent on manual cleaning after each processing, further reducing the overall pressing efficiency. In addition, the structural design is not targeted enough and has poor adaptability. The device was originally designed for camellia seeds, but the fruit structure, water content, and fiber content of monk fruit are significantly different from those of camellia seeds. Directly applying the device will result in a mismatch between the pressing pressure, pressing rate, and other parameters and the monk fruit raw material, which may lead to insufficient or excessive pressing, resulting in the loss of effective components. This, in turn, affects the extraction rate and purity of mogroside V. It is evident that the existing pressing device, when applied to the large-scale extraction of monk fruit, suffers from problems such as cumbersome operation, time-consuming cleaning, low production efficiency, and insufficient adaptability. It is difficult to meet the requirements of continuous, efficient, and clean production for the extraction of high-purity mogroside V. Therefore, there is an urgent need for targeted improvements to the pressing device design. Summary of the Invention

[0004] In order to improve the production and processing efficiency and purification effect during the application of existing technologies, this application provides a production method for extracting high-purity mogroside V from monk fruit.

[0005] This application provides a method for extracting high-purity mogroside V from monk fruit, which adopts the following technical solution: including the following steps:

[0006] Step 1: Fresh fruit pretreatment and enzyme inactivation. Mature fruits are selected by infrared spectroscopy fruit sorting machine, washed with clean water, then crushed and added with a vitamin C solution of 20-40% of the fruit weight. The vitamin C concentration in the solution is 0.04%-0.06%. At the same time, high-temperature steam is introduced to inactivate enzymes at a temperature of 100-110℃.

[0007] Step 2: Multiple pressing. The processed raw material is fed into the pressing equipment and subjected to three gradient pressing operations. The pressure of the first pressing is controlled at 0.2MPa-0.4MPa, the second at 0.4MPa-0.6MPa, and the third at 0.6MPa-0.8MPa. After each pressing, the juice is collected and combined to obtain the juice.

[0008] Step 3: Compound enzyme-assisted countercurrent extraction of fruit pomace. Put the remaining fruit pomace after pressing into a continuous countercurrent extraction device. The extraction temperature is 30-45℃, the extraction solvent is a 0.1-0.3% concentration of compound enzyme aqueous solution, the amount of solvent is 0.5-1 times the weight of monk fruit, and the extraction time is 20-60 minutes.

[0009] Step 4: Compound clarifying agent treatment. Combine the juice obtained by pressing with the extract obtained by countercurrent extraction, then adjust the pH of the mixture to 4-6, stir for 30-60 minutes at 30-50℃, add chitosan-benzene compound clarifying agent, stir the mixture, and then use a horizontal screw centrifuge in series with a disc centrifuge to separate the precipitate and collect the centrifuged liquid.

[0010] Step 5: Simulated moving bed purification. The centrifuged liquid is pumped into a simulated moving bed chromatography system to separate sugars, glycosides and pigments, and collect the target components. The sugar-enriched components are sent to a cation exchange resin for treatment and concentration to obtain monk fruit concentrate product.

[0011] Step Six: Nanofiltration Membrane Concentration and Desalination. The collected target components are sent to a nanofiltration membrane system for concentration. The solution is concentrated until the solid content reaches 10-15%, and then sent to vacuum concentration at a temperature of 50-70℃ until the solid content reaches 30-40%.

[0012] Step 7: Spray drying and finished product testing. The concentrated liquid is sent to a spray drying tower for drying. After drying, a white powdery finished product is obtained. The purity of glycoside V in the finished product is tested by high performance liquid chromatography. After all indicators meet the food additive standards, the product is packaged and stored.

[0013] Optionally, in step four, a chitosan-benzene composite clarifying agent is added at a ratio of 0.08%-0.12% of the mixture mass, wherein the mass ratio of chitosan to bentonite is 1:3, and the mixture is stirred at an environment of 30-50℃.

[0014] Optionally, in step five, the simulated moving bed chromatography system consists of 12 chromatographic columns packed with anion exchange resin packing material, the eluent is set to a gradient ethanol aqueous solution of 0-35%, the feed flow rate is controlled at 2BV / h-4BV / h, and the elution flow rate is 1BV / h-2BV / h.

[0015] Optionally, in step six, the nanofiltration membrane used in the nanofiltration membrane system has a molecular weight cutoff of 500-1000 Da, and during operation, the pressure is controlled at 1 MPa-2 MPa and the temperature at 35℃-45℃.

[0016] Optionally, in step seven, the inlet air temperature of the spray drying tower is set to 175-200℃, and the outlet air temperature is set to 70-95℃. When using high performance liquid chromatography for detection, the moisture and ash content of the finished product are also tested, requiring that the moisture content be ≤5% and the ash content be ≤0.5%. After all indicators meet the food additive standards, the product is packaged and stored.

[0017] Optionally, the pressing equipment includes a collection tank, a fixing ring is fixedly installed on the top outer side of the collection tank, an annular groove is opened on the outer side of the fixing ring, a plurality of sliding plates are slidably connected inside the annular groove, a driving mechanism is fixedly installed on the outer side of the sliding plates, a pressing bearing mechanism is fixedly installed on the top of each driving mechanism, a pressing pressure mechanism is fixedly installed in the middle of the rear side of the collection tank, a cleaning mechanism is fixedly installed on the front of the collection tank, a conical guide hopper is fixedly installed at the bottom of the collection tank, and a discharge valve is fixedly installed at the bottom of the conical guide hopper.

[0018] The driving mechanism includes a gear ring and a fixed plate. The fixed plate is fixedly installed on the top of the slide plate outside the slide groove. The fixed plate is slidably connected to the outside of the fixed ring. The gear ring is fixedly installed on the bottom outside of the fixed ring. A first motor is fixedly installed at the top outer end of the fixed plate. A gear is fixedly installed through the fixed plate at the output end of the first motor. The gear and the gear ring are meshed and connected. The pressing bearing mechanism is fixedly installed on the top inner side of the fixed plate.

[0019] Optionally, the overall top view shape of the slide plate is arc-shaped, the top view shape of the slide plate and the annular groove are concentric circles, and the overall cross-sectional shape of the slide plate and the cross-sectional shape of the internal cavity of the annular groove are both convex.

[0020] Optionally, the pressing support mechanism includes a support plate, which is fixedly installed on the top inner side of a fixed plate. A mounting frame is fixedly installed on the top of the support plate, and a support component is fixedly installed on the top of the mounting frame. Side plates are fixedly installed at both ends inside the mounting frame. A pressing barrel is rotatably connected to the inner side of the side plate. A second motor is fixedly installed on the outer side of one side plate, and the output end of the second motor passes through the side plate and is fixedly connected to one side of the upper end of the pressing barrel.

[0021] Optionally, a conical lower pressing platform is fixedly installed in the middle of the bottom of the pressing barrel, and leakage holes are arranged in a ring at equal intervals on the outer side of the bottom of the pressing barrel, the leakage holes penetrating the pressing barrel, and reinforcing frames are fixedly installed at both ends of the bottom of the mounting frame, the bottom of the reinforcing frame and the top two sides of the fixing plate are fixedly connected.

[0022] Optionally, the support assembly includes a top rail and a support frame. The top rail is fixedly installed in the middle of the top of the mounting frame. A third motor is fixedly installed at the outer end of the top rail. A lead screw is fixedly installed through the top rail at the output end of the third motor. The lead screw is rotatably connected to the inside of the top rail. A movable block is threadedly connected to the outer surface of the lead screw. A support frame is fixedly installed on the side of the movable block near the pressing barrel. A support block is fixedly installed at the end of the support frame. The support frame is fixedly installed on the upper end of the pressing barrel near the third motor. The end of the support block is inserted into the inner side of the support frame.

[0023] The pressing mechanism includes a fixed arm, which is fixedly installed on the upper back of the collection tank. A fixed base is fixedly installed at the front end of the fixed arm, and a hydraulic cylinder is fixedly installed on the top of the fixed base. The output end of the hydraulic cylinder passes through the fixed base and is fixedly installed with a pressing head. A conical groove is opened at the bottom of the pressing head, and the top of the conical lower pressing platform is inserted into the inside of the conical groove and is in close contact with the inner wall of the conical groove.

[0024] In summary, this application includes the following beneficial technical effects:

[0025] 1. This application achieves continuous operation of the monk fruit pressing process by adopting a multi-station circulating pressing design, effectively avoiding production interruptions caused by manual cleaning of residues during single-station operation, greatly improving pressing efficiency, reducing operation time and labor costs, and meeting the demand for high efficiency in large-scale production.

[0026] 2. By combining compound enzyme-assisted countercurrent extraction with compound clarifying agent treatment, the extraction rate and purity of mogroside V were significantly improved. The enzymatic hydrolysis process fully released the effective components, and the clarifying agent effectively removed impurities, ensuring the quality of raw materials for subsequent purification steps and laying a solid foundation for obtaining high-purity products.

[0027] 3. Purification was performed using a simulated moving bed chromatography system, which achieved efficient separation of sugars, glycosides and pigments. By precisely controlling the elution gradient and flow rate, the purity of mogroside V in the target component was ensured to reach 80% or more, thereby improving the quality and market competitiveness of the product.

[0028] 4. The equipment integrates an automatic cleaning mechanism, which can automatically flip, scrape and discharge the pressing barrel after pressing, thoroughly remove residues, avoid cross-contamination and microbial growth, ensure the hygiene and safety of production and product stability, and significantly reduce cleaning time and manual intervention.

[0029] 5. This application sequentially employs enzyme inactivation treatment to prevent oxidation, gradient pressure pressing to fully extract juice, compound enzymatic hydrolysis-assisted countercurrent extraction of residual active ingredients, compound clarifying agent to remove impurities, simulated moving bed chromatography to accurately separate target components, nanofiltration membrane concentration and desalting, and spray drying to obtain powder, ultimately yielding a high-purity product. This method achieves continuous and efficient production from raw materials to finished products, significantly improving the extraction rate and product purity of mogroside V, while ensuring the stability of the production process and the consistency of product quality, effectively solving the problems of low efficiency, low purity, and production interruption in traditional processes. Attached Figure Description

[0030] Figure 1 This is a schematic diagram of the overall structure in an embodiment of this application;

[0031] Figure 2 This is a side view of the structure in an embodiment of this application;

[0032] Figure 3 This is a top view of the structure in an embodiment of this application;

[0033] Figure 4 This is a bottom-view structural diagram of an embodiment of this application;

[0034] Figure 5 This is a schematic diagram of the cleaning mechanism structure in an embodiment of this application;

[0035] Figure 6 This is a schematic diagram of the overturned state structure of the pressing support mechanism in the embodiments of this application;

[0036] Figure 7 This is a schematic diagram of the pressing support mechanism in the unflipped state in an embodiment of this application;

[0037] Figure 8 This is a bottom view of the pressing support mechanism in an embodiment of this application;

[0038] Figure 9 This is a schematic diagram of the internal structure of the pressing barrel in an embodiment of this application.

[0039] Reference numerals: 1. Collection tank; 2. Fixing ring; 3. Drive mechanism; 31. Gear ring; 32. Fixing plate; 33. First motor; 34. Gear; 4. Slide plate; 5. Annular groove; 6. Pressing bearing mechanism; 61. Support plate; 62. Mounting frame; 63. Support assembly; 631. Top rail; 632. Support frame; 633. Third motor; 634. Lead screw; 635. Movable block; 636. Support frame; 637. Support block; 64. Side plate; 65. Pressing barrel; 66. Second motor; 67. Conical lower pressing table; 6 8. Drainage hole; 69. Reinforcing frame; 7. Pressing mechanism; 71. Fixed arm; 72. Fixed seat; 73. Hydraulic cylinder; 74. Pressing head; 75. Conical groove; 8. Discharge valve; 9. Cleaning mechanism; 91. Waste residue guide discharge frame; 92. Cleaning module; 921. Concave frame; 922. Electric push rod; 923. Base frame; 924. Fourth motor; 925. Rotating shaft; 926. Cleaning scraping auger; 93. Support leg; 94. Support bottom ring; 95. Collection frame; 96. Waste collection box; 10. Conical guide hopper. Detailed Implementation

[0040] The following is in conjunction with the appendix Figure 1-9 This application will be described in further detail.

[0041] This application discloses a method for extracting high-purity mogroside V from monk fruit. For example... Figure 1-8 As shown, it includes the following steps:

[0042] Step 1: Fresh fruit pretreatment and enzyme inactivation. Mature fruits are selected using an infrared spectroscopy fruit sorting machine. The surface fuzz and impurities of the fruits are washed away with clean water. Then, the fruits are crushed while ensuring the integrity of the seeds. During crushing, a vitamin C solution of 20-40% of the fruit weight is added. The vitamin C concentration in the solution is 0.04%-0.06% to avoid high-temperature oxidation of phenolic substances and reduce the difficulty of subsequent decolorization. At the same time, high-temperature steam is introduced to inactivate the enzymes in the crushed raw materials at a temperature of 100-110℃.

[0043] Step 2: Multiple pressing. The processed raw material is fed into the pressing equipment and subjected to three gradient pressing operations. The pressure of the first pressing is controlled at 0.2MPa-0.4MPa, the second at 0.4MPa-0.6MPa, and the third at 0.6MPa-0.8MPa. After each pressing, the juice is collected and combined to obtain the juice.

[0044] Step 3: Compound enzyme-assisted countercurrent extraction of fruit pomace. Put the remaining fruit pomace after pressing into a continuous countercurrent extraction device. The extraction temperature is 30-45℃, and the extraction solvent is a 0.1-0.3% concentration of compound enzyme aqueous solution (pectinase, cellulase, protease, lipase). The amount of solvent used is 0.5-1 times the weight of monk fruit, and the extraction time is 20-60 minutes.

[0045] Step 4: Compound clarifying agent treatment. Combine the juice obtained from pressing with the extract obtained from countercurrent extraction, then adjust the pH of the mixture to 4-6, and stir for 30-60 minutes at 30-50℃ for complete enzymatic hydrolysis. Add chitosan-benzene compound clarifying agent at a ratio of 0.08%-0.12% of the mixture mass, where the mass ratio of chitosan to bentonite is 1:3. Stir the mixture at 30-50℃ for 20-40 minutes, and then separate using a horizontal screw centrifuge connected to a disc centrifuge. The speed of the horizontal screw centrifuge is 1500r / min-3000r / min, and the speed of the disc centrifuge is set to 4000r / min-5000r / min. Separate the precipitate and collect the centrifuged liquid.

[0046] Step 5: Simulated moving bed purification. The centrifuged liquid is pumped into a simulated moving bed chromatography system. This system consists of 12 chromatographic columns packed with anion exchange resins (HP613, LX-94, LS-807E). The eluent is set to a gradient of 0-35% ethanol-water solution. The feed flow rate is controlled at 2 BV / h-4 BV / h, and the elution flow rate is controlled at 1 BV / h-2 BV / h. By controlling the column switching time and elution gradient, efficient separation of sugars, glycosides, and pigments is achieved. The target components are collected, ensuring that the purity of glycoside V in the components reaches 80% or higher. The sugar-enriched components can be sent to cation exchange resin for treatment. Concentration yields a colorless to light yellow monk fruit concentrated juice product with a solid content of more than 65% and rich in fructose, glucose, and sucrose.

[0047] Step Six: Nanofiltration Membrane Concentration and Desalination. The collected target components are sent to a nanofiltration membrane system for concentration. The nanofiltration membrane used has a molecular weight cutoff of 500-1000 Da. During operation, the pressure is controlled at 1 MPa-2 MPa and the temperature at 35℃-45℃. The solution is concentrated until the solid content reaches 10-15%, and then sent to a reduced pressure for concentration. The solution is concentrated until the solid content reaches 30-40% at a temperature of 50-70℃.

[0048] Step 7: Spray drying and finished product testing. The concentrated liquid is sent to a spray drying tower for drying. The inlet air temperature of the spray drying tower is set to 175-200℃, and the outlet air temperature is 70-95℃. After drying, a white powdery finished product is obtained. The purity of glycoside V in the finished product is tested by high performance liquid chromatography. The purity is required to be 80% or higher. In actual production batches, the purity can reach 82% to 88%. At the same time, the moisture and ash content of the finished product are tested. The moisture content is required to be ≤5%, and the ash content is required to be ≤0.5%. After all indicators meet the food additive standards, the product is packaged and stored.

[0049] Please refer to Figures 1-9The pressing equipment includes a collection tank 1, a fixing ring 2 fixedly installed on the top outer side of the collection tank 1, an annular groove 5 opened on the outer side of the fixing ring 2, a number of sliding plates 4 slidably connected inside the annular groove 5, a drive mechanism 3 fixedly installed on the outer side of the sliding plates 4, a pressing bearing mechanism 6 fixedly installed on the top of each drive mechanism 3, a pressing pressure mechanism 7 fixedly installed in the middle of the rear side of the collection tank 1, a cleaning mechanism 9 fixedly installed on the front of the collection tank 1, a conical guide bucket 10 fixedly installed at the bottom of the collection tank 1, and a discharge valve 8 fixedly installed at the bottom of the conical guide bucket 10.

[0050] The drive mechanism 3 includes a gear ring 31 and a fixing plate 32. The fixing plate 32 is fixedly installed on the top of the slide plate 4 outside the slide groove. The fixing plate 32 is slidably connected to the outside of the fixing ring 2. The gear ring 31 is fixedly installed on the bottom outside of the fixing ring 2. A first motor 33 is fixedly installed at the top outer end of the fixing plate 32. A gear 34 is fixedly installed through the fixing plate 32 at the output end of the first motor 33. The gear 34 and the gear ring 31 are meshed together. The pressing and bearing mechanism 6 is fixedly installed on the top inner side of the fixing plate 32. During the application of this device, the collection tank 1 serves as the basic bearing structure. The fixed ring 2 on the top outer side provides stable track support for the entire equipment's workstation movement. When the monk fruit needs to be pressed, the worker puts the processed raw material into the pressing bearing mechanism 6, and then the drive mechanism 3 starts to operate. The first motor 33 at the top outer end of the fixed plate 32 starts, and its output end drives the gear 34 to rotate. Since the gear 34 meshes with the toothed ring 31 fixed on the bottom outer side of the fixed ring 2, the rotation of the gear 34 is converted into the sliding of the fixed plate 32 along the outer side of the fixed ring 2, while the slide plate 4 slides synchronously with the fixed plate 32 in the annular groove 5. This causes the pressing support mechanism 6 to move along a predetermined trajectory. When the pressing support mechanism 6 moves to the middle of the rear side of the collection tank 1, below the pressing pressure mechanism 7, the pressing pressure mechanism 7 performs pressing operations on the raw material. The resulting juice falls into the collection tank 1, is collected by the conical guide bucket 10 at the bottom, and is finally transported to the next process through the discharge valve 8. During this process, multiple pressing support mechanisms 6 can circulate on the annular groove 5 through the drive mechanism 3. When one pressing support mechanism 6 is in the pressing position, other pressing support mechanisms 6 can be in different positions. This design enables continuous operation of multiple workstations, avoiding downtime during single-station operation and significantly improving production efficiency. At the same time, the sliding cooperation between the slide plate 4 and the annular groove 5 ensures the smooth movement of the pressing support mechanism 6, ensuring the precision of the pressing operation and reducing raw material waste and juice overflow. After pressing is completed, the drive mechanism 3 continues to move the pressing support mechanism 6 to the cleaning mechanism 9 in front of the collection tank 1, preparing for subsequent cleaning operations and further ensuring the continuity and stability of equipment operation.

[0051] Please refer to Figures 1-5The cleaning mechanism 9 includes a pressing waste guide discharge frame 91, which is fixedly installed at the front end of the collection tank 1. The pressing waste guide discharge frame 91 is inclined, and a cleaning module 92 is fixedly installed inside the pressing waste guide discharge frame 91. The pressing barrel 65 on the side near the cleaning module 92 is in an inverted state, and the cleaning module 92 is inserted into the pressing barrel 65 on the side near the pressing waste guide discharge frame 91. The cleaning mechanism 9 also includes a collection frame 95 and support legs 93. The support legs 93 are arranged in a ring at equal intervals and fixedly installed on the outside of the collection tank 1. A support bottom ring 94 is fixedly installed at the bottom of the support legs 93, and the collection frame 95 is fixedly installed at the top front end of the support bottom ring 94. A waste collection box 96 is slidably connected inside the collection frame 95. The waste collection box 96 is located directly below the output end of the pressing waste discharge frame 91. The cleaning module 92 includes a concave frame 921, which is fixedly installed on the inner front end of the pressing waste discharge frame 91. The concave frame 921 and the pressing waste discharge frame 91 are inclined together. An electric push rod 922 is fixedly installed on the inner side of the concave frame 921. A base frame 923 is fixedly installed at the end of the electric push rod 922. A fourth motor 924 is fixedly installed on the inner side of the base frame 923. A rotating shaft 925 is fixedly installed at the output end of the fourth motor 924. A cleaning scraping auger 926 is fixedly installed at the end of the rotating shaft 925. The cleaning auger 926 is inserted into the inner wall of the downward-facing pressing barrel 65 and is in close contact with the inner wall of the pressing barrel and the outer wall of the conical lower pressing platform 67. During the operation of this device, when the pressing barrel 65 finishes pressing and moves to the cleaning mechanism 9, the pressing barrel 65 is in a flipped state with its opening facing the inclined pressing waste guide discharge frame 91. At this time, the cleaning module 92 starts to operate, and the electric push rod 922 inside the concave frame 921 is activated, pushing the base frame 923 to move, so that the cleaning scraping auger 926 is inserted into the downward-facing pressing barrel 65. Since the cleaning scraping auger 926 is in close contact with the inner wall of the pressing barrel 65 and the outer wall of the conical lower pressing platform 67, when the fourth motor 924 drives the rotating shaft 925 to rotate, the auger can clean the residual juice. The machine thoroughly scrapes away fine residue. Under the influence of gravity, the scraped waste slides down the inclined waste discharge frame 91 and finally falls into the waste collection box 96 directly below its output end. The collection frame 95 provides a stable placement space for the waste collection box 96. The support legs 93 and the support bottom ring 94 cooperate to provide stable support for the entire cleaning mechanism 9 and the collection pool 1, ensuring that the equipment will not shake during the cleaning process. This design allows the cleaning of the pressing barrel 65 to be done without manual intervention. The inclined discharge frame accelerates the discharge of waste, and the close-fitting auger ensures thorough cleaning, effectively reducing cleaning time and avoiding the impact of residue residue on the purity of subsequent pressing raw materials, further improving the continuity and efficiency of production.

[0052] Please refer to Figures 6-9The slide plate 4 has an arc-shaped overall top view. The slide plate 4 and the annular groove 5 are arranged in concentric circles. The overall cross-sectional shape of the slide plate 4 and the cross-sectional shape of the internal cavity of the annular groove 5 are both convex. The pressing bearing mechanism 6 includes a support plate 61, which is fixedly installed on the top inner side of the fixed plate 32. A mounting frame 62 is fixedly installed on the top of the support plate 61. A support component 63 is fixedly installed on the top of the mounting frame 62. Side plates 64 are fixedly installed at both ends inside the mounting frame 62. The pressing barrel 65 is rotatably connected to the inner side of the side plate 64. A second motor 66 is fixedly installed on the outer side of one side plate 64. The output end of the second motor 66 passes through the side plate 64 and... The upper side of the pressing barrel 65 is fixedly connected. A conical lower pressing platform 67 is fixedly installed in the middle of the bottom of the pressing barrel 65. Drainage holes 68 are arranged in a ring at equal intervals on the outer side of the bottom of the pressing barrel 65, and the drainage holes 68 penetrate the pressing barrel 65. Reinforcing frames 69 are fixedly installed at both ends of the bottom of the mounting frame 62. The bottom of the reinforcing frame 69 is fixedly connected to the top sides of the fixing plate 32. During the operation of this device, the sliding plate 4, with its arc-shaped top view, forms a concentric circle with the annular groove 5. The convex cross-section design makes the two fit more tightly, and there will be no displacement or jamming during the sliding process, providing stable support for the movement of the pressing bearing mechanism 6. When the drive mechanism 3 moves the pressing support mechanism 6, the support plate 61 integrates the components into a whole through the mounting frame 62. The reinforcing frame 69 further enhances the stability of the connection between the mounting frame 62 and the fixed plate 32, maintaining structural stability even under high pressure during pressing. When the pressing support mechanism 6 reaches the pressing position, the support component 63 fixes the pressing barrel 65 to ensure that the barrel does not shake during the pressing process. At this time, the pressing pressure mechanism 7 applies downward pressure, and the conical lower pressing platform 67 inside the pressing barrel 65 cooperates with the pressure application component to uniformly squeeze the monk fruit raw material. The squeezed juice flows along the inclined surface of the conical lower pressing platform 67 and finally passes through... The liquid is quickly discharged through the annularly arranged drainage holes 68 at the bottom, avoiding the waste of raw materials caused by the stagnation of juice in the barrel. After pressing is completed, the second motor 66 starts, and its output end drives the pressing barrel 65 to rotate around the side plate 64, realizing the barrel flipping. During this process, the side plate 64 provides a stable rotation fulcrum for the pressing barrel 65, ensuring that the flipping action is precise and controllable. After flipping, the pressing barrel 65 can smoothly discharge the residue. The cooperation between the arc-shaped sliding plate 4 and the annular groove 5 ensures that the entire pressing bearing mechanism 6 remains stable during movement and operation, effectively improving the continuity and reliability of equipment operation, reducing production interruptions caused by structural instability, and further improving production efficiency.

[0053] Please refer to Figures 6-9The support assembly 63 includes a top rail 631 and a support frame 632. The top rail 631 is fixedly installed in the middle of the top of the mounting frame 62. A third motor 633 is fixedly installed at the outer end of the top rail 631. A lead screw 634 is fixedly installed through the top rail 631 at the output end of the third motor 633. The lead screw 634 is rotatably connected to the inside of the top rail 631. A movable block 635 is threadedly connected to the outer surface of the lead screw 634. A support frame 636 is fixedly installed on the side of the movable block 635 near the pressing barrel 65. A support block 637 is fixedly installed at the end of the support frame 636. The support frame 632 is fixedly installed on the upper end of the pressing barrel 65 near the third motor 633. The end of the support block 637 is inserted into the inner side of the support frame 632. The pressing and applying mechanism 7 includes... A fixed arm 71 is fixedly installed on the upper back of the collection tank 1. A fixed base 72 is fixedly installed at the front end of the fixed arm 71. A hydraulic cylinder 73 is fixedly installed on the top of the fixed base 72. The output end of the hydraulic cylinder 73 passes through the fixed base 72 and is fixedly installed with a pressing head 74. A conical groove 75 is opened at the bottom of the pressing head 74. The top of the conical lower pressing table 67 is inserted into the conical groove 75 and is in close contact with the inner wall of the conical groove 75. During the application of this device, when the pressing bearing mechanism 6 moves to the pressing position, the support component 63 starts to work, the third motor 633 starts, and its output end drives the lead screw 634 to rotate inside the top rail 631. Since the lead screw 634 is threadedly connected to the movable block 635, the movable block 635 rotates with the lead screw 634. The cylinder moves along the top rail 631 toward the pressing barrel 65. The support frame 636 pushes the support block 637 into the inner side of the support sleeve 632, thus firmly fixing the pressing barrel 65. This fixing method can be precisely adjusted according to the position of the pressing barrel 65, ensuring that the barrel will not shift or shake due to force during the pressing process, providing a stable foundation for subsequent pressing operations. Then, the pressing and applying mechanism 7 starts operating. The fixed arm 71 and fixed seat 72 provide stable support for the hydraulic cylinder 73. After the hydraulic cylinder 73 starts, its output end pushes the pressing head 74 downwards. The conical groove 75 at the bottom of the pressing head 74 precisely fits with the top of the conical lower pressing platform 67 inside the pressing barrel 65, forming a complete extrusion space. As the hydraulic cylinder 73 continues to apply pressure, the conical groove 75... The conical lower pressing platform 67, in conjunction with the pressing platform, ensures that pressure is applied evenly to the monk fruit raw material. This not only fully extracts the juice but also prevents excessive crushing of the raw material or loss of effective components due to excessive local pressure. The juice flows rapidly along the inclined surface of the conical lower pressing platform 67 to the drain hole 68. The entire pressing process is efficient and stable, reducing raw material waste and loss of effective components, further improving pressing efficiency and juice quality. After pressing is completed, the third motor 633 reverses, driving the lead screw 634 to rotate in the opposite direction. The movable block 635 drives the support block 637 to disengage from the support frame 632, releasing the fixation on the pressing barrel 65 and preparing for subsequent residue discharge and cleaning operations. This automated fixing and unlocking method reduces manual operation steps and makes the equipment operation more seamless.This effectively improved overall production efficiency.

[0054] The implementation principle of the production method for extracting high-purity mogroside V from monk fruit according to an embodiment of this application is as follows: During the application of this equipment, when performing monk fruit pressing operations, the crushed monk fruit raw material must first be put into one of the pressing barrels 65. At this time, several pressing bearing mechanisms 6 are distributed in the annular groove 5 of the top fixing ring 2 of the collection tank 1 via sliding plates 4, forming multiple independently operable units. The first motor 33 of the drive mechanism 3 is started, and its output end drives the gear 34 to rotate. The gear 34 meshes with the toothed ring 31 at the bottom of the fixing ring 2, and the resulting driving force drives the fixing plate 32 to slide along the outside of the fixing ring 2. The sliding plate 4 then slides smoothly in the annular groove 5, accurately moving the pressing barrel 65 containing the raw material directly below the pressing and applying mechanism 7. During this process, the annular groove 5 of the fixing ring 2 provides a stable movement trajectory for the sliding plate 4, ensuring the pressing... The pressing barrel 65 will not shift during the relocation process, ensuring the accuracy of subsequent pressing operations. The setting of multiple pressing support mechanisms 6 allows the raw material feeding and workstation switching to be carried out synchronously. When one pressing barrel 65 is performing a pressing operation, other pressing barrels 65 can simultaneously complete the raw material loading or relocation, avoiding production interruptions during single-workstation operation and fundamentally solving the problem of frequent manual shutdowns required in the existing technology. When the pressing barrel 65 reaches the designated position, the third motor 633 of the support component 63 starts, driving the lead screw 634 to rotate, causing the movable block 635 to move directionally along the top rail 631. The movable block 635 pushes the support block 637 through the support frame 636 to accurately insert into the support sleeve frame 632, thereby achieving stable fixation of the pressing barrel 65, providing a solid foundation for subsequent pressing operations and avoiding the impact of barrel shaking on the pressing effect during the pressing process.

[0055] Next, the pressing stage begins. The hydraulic cylinder 73 of the pressing mechanism 7 is activated, pushing the pressing head 74 to slowly descend. The conical groove 75 at the bottom of the pressing head 74 cooperates with the conical lower pressing platform 67 inside the pressing barrel 65 to form a fitting compression space, uniformly pressing the monk fruit raw material. The conical structure of the conical groove 75 and the conical lower pressing platform 67 guides the pressed liquid to flow in all directions, eventually collecting at the drain hole 68 for rapid juice discharge. The juice produced during pressing flows into the collection tank 1 through the drain hole 68 at the bottom of the pressing barrel 65. The structural design of the collection tank 1 ensures that the juice does not overflow, and all juice is guided by the conical groove 75 at the bottom. The liquid is collected in the hopper 10 and then discharged in an orderly manner to the subsequent process through the discharge valve 8. The fixed arm 71 and the fixed seat 72 provide stable support for the hydraulic cylinder 73, ensuring that the pressure remains stable during the pressing process and avoiding the impact of pressure fluctuations on the juice extraction efficiency. This targeted structural design can well adapt to the physical characteristics of monk fruit, reduce the loss of effective ingredients during the pressing process, and thus improve the extraction rate and purity. It effectively improves the problem of insufficient adaptability of existing equipment. During the pressing process, the reinforcing frame 69 at the bottom of the mounting frame 62 further enhances the stability of the overall structure, can withstand greater pressing pressure, and avoids the equipment from deforming due to long-term stress.

[0056] After pressing is completed, the hydraulic cylinder 73 drives the pressing head 74 to rise and reset. The third motor 633 of the support assembly 63 reverses, causing the support block 637 to disengage from the support frame 632, releasing the fixation of the pressing barrel 65. Subsequently, the first motor 33 starts again, driving the pressing bearing mechanism 6 to move and move the pressing barrel 65 to the pressing waste discharge guide frame 91. After reaching the position, the second motor 66 is started, and its output end drives the pressing barrel 65 to rotate around the side plate 64, causing the pressing barrel 65 to flip so that the opening faces the pressing waste discharge guide frame 91. At this time, the residue in the barrel falls naturally into the discharge frame under the action of gravity, and falls into the waste collection box 96 under the guidance of the discharge frame. This automatic slag discharge process requires no manual intervention, greatly reducing the operation steps and solving the problem of tedious manual slag removal in the prior art. After the slag discharge is completed, the first motor 33 continues to drive the pressing bearing mechanism 6 to move, so that the flipped pressing barrel 65 reaches the cleaning position. The system has nine cleaning mechanisms. The electric push rod 922 of the cleaning module 92 is activated, pushing the mounting frame 62 to move, allowing the cleaning scraping auger 926 to accurately insert into the pressing barrel 65. The fourth motor 924 drives the rotating shaft 925 to rotate, and the cleaning scraping auger 926 fits tightly against the inner wall of the pressing barrel 65 and the outer wall of the conical lower pressing platform 67, thoroughly scraping and cleaning the residual juice and small residues. The waste generated during cleaning is guided by the pressing waste residue to the discharge frame 91 and enters the waste collection box 96, ensuring that there are no impurities left inside the pressing barrel 65. After cleaning, the electric push rod 922 drives the cleaning scraping auger 926 to reset, and the second motor 66 drives the pressing barrel 65 to rotate back to the initial position, waiting for the next operation. The support legs 93 and the support bottom ring 94 provide stable support for the entire equipment, ensuring that the mechanisms do not shake during operation. The automatic cleaning function eliminates the need for manual cleaning, greatly shortens the cleaning time, and significantly improves production efficiency.

[0057] Throughout the production process, each pressing support mechanism 6 operates cyclically according to a predetermined process. When one pressing tank 65 is pressing, discharging residue, or cleaning, other pressing support mechanisms 6 can simultaneously feed raw materials or move to the corresponding workstation, forming a continuous production process. The collection tank 1 can effectively receive all the juice produced by pressing, ensuring the integrity of the juice collection and avoiding raw material waste. The various structures work together, and through automated workstation switching, continuous operation, and automatic cleaning design, not only is the dependence on manual labor reduced and labor costs lowered, but the operation interruption time is also significantly reduced, resulting in a significant increase in the pressing volume per unit time. At the same time, the targeted design of each structure fundamentally solves the problems of cumbersome operation, low efficiency, time-consuming cleaning, and insufficient adaptability in the existing technology, and can meet the requirements of high-purity monk fruit glycoside V extraction process for production efficiency and product quality.

[0058] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A method for extracting high-purity mogroside V from monk fruit, characterized in that; Includes the following steps: Step 1: Fresh fruit pretreatment and enzyme inactivation. Mature fruits are selected by infrared spectroscopy fruit sorting machine, washed with clean water, then crushed and added with a vitamin C solution of 20-40% of the fruit weight. The vitamin C concentration in the solution is 0.04%-0.06%. At the same time, high-temperature steam is introduced to inactivate enzymes at a temperature of 100-110℃. Step 2: Multiple pressing. The processed raw material is fed into the pressing equipment and subjected to three gradient pressing operations. The pressure of the first pressing is controlled at 0.2MPa-0.4MPa, the second at 0.4MPa-0.6MPa, and the third at 0.6MPa-0.8MPa. After each pressing, the juice is collected and combined to obtain the juice. Step 3: Compound enzyme-assisted countercurrent extraction of fruit pomace. Put the remaining fruit pomace after pressing into a continuous countercurrent extraction device. The extraction temperature is 30-45℃, the extraction solvent is a 0.1-0.3% concentration of compound enzyme aqueous solution, the amount of solvent is 0.5-1 times the weight of monk fruit, and the extraction time is 20-60 minutes. Step 4: Compound clarifying agent treatment. Combine the juice obtained by pressing with the extract obtained by countercurrent extraction, then adjust the pH of the mixture to 4-6, stir for 30-60 minutes at 30-50℃, add chitosan-benzene compound clarifying agent, stir the mixture, and then use a horizontal screw centrifuge in series with a disc centrifuge to separate the precipitate and collect the centrifuged liquid. Step 5: Simulated moving bed purification. The centrifuged liquid is pumped into a simulated moving bed chromatography system to separate sugars, glycosides and pigments, and the target components are collected. The sugar-enriched components are sent to a cation exchange resin for treatment and concentration to obtain monk fruit concentrate product. Step Six: Nanofiltration Membrane Concentration and Desalination. The collected target components are sent to a nanofiltration membrane system for concentration. The solution is concentrated until the solid content reaches 10-15%, and then sent to vacuum concentration at a temperature of 50-70℃ until the solid content reaches 30-40%. Step 7: Spray drying and finished product testing. The concentrated liquid is sent into a spray drying tower for drying. After drying, a white powdery finished product is obtained. The purity of glycoside V in the finished product is tested by high performance liquid chromatography. After all indicators meet the food additive standards, the product is packaged and stored. The pressing equipment includes a collection tank (1), a fixing ring (2) is fixedly installed on the outer top of the collection tank (1), an annular groove (5) is opened on the outer side of the fixing ring (2), a number of sliding plates (4) are slidably connected inside the annular groove (5), a driving mechanism (3) is fixedly installed on the outer side of the sliding plate (4), a pressing bearing mechanism (6) is fixedly installed on the top of the driving mechanism (3), a pressing pressure mechanism (7) is fixedly installed on the middle of the rear side of the collection tank (1), a cleaning mechanism (9) is fixedly installed on the front of the collection tank (1), a conical guide bucket (10) is fixedly installed at the bottom of the collection tank (1), and a discharge valve (8) is fixedly installed at the bottom of the conical guide bucket (10). The drive mechanism (3) includes a gear ring (31) and a fixing plate (32). The fixing plate (32) is fixedly installed on the top of the slide plate (4) outside the slide groove. The fixing plate (32) is slidably connected to the outside of the fixing ring (2). The gear ring (31) is fixedly installed on the bottom outside of the fixing ring (2). A first motor (33) is fixedly installed on the top outer end of the fixing plate (32). A gear (34) is fixedly installed through the fixing plate (32) at the output end of the first motor (33). The gear (34) and the gear ring (31) are meshed and connected. The pressing bearing mechanism (6) is fixedly installed on the top inner side of the fixing plate (32). The pressing support mechanism (6) includes a support plate (61), which is fixedly installed on the top inner side of the fixed plate (32). A mounting frame (62) is fixedly installed on the top of the support plate (61). A support component (63) is fixedly installed on the top of the mounting frame (62). Side plates (64) are fixedly installed at both ends of the mounting frame (62). A pressing barrel (65) is rotatably connected to the inner side of the side plate (64). A second motor (66) is fixedly installed on the outer side of one side plate (64). The output end of the second motor (66) is fixedly connected through the side plate (64) and the upper side of the pressing barrel (65). The support assembly (63) includes a top rail (631) and a support frame (632). The top rail (631) is fixedly installed at the top center of the mounting frame (62). A third motor (633) is fixedly installed at the outer end of the top rail (631). A lead screw (634) is fixedly installed through the top rail (631) at the output end of the third motor (633). The lead screw (634) is rotatably connected to the inside of the top rail (631). A movable block (635) is threadedly connected to the outer surface of the lead screw (634). A support frame (636) is fixedly installed on the side of the movable block (635) near the pressing barrel (65). A support block (637) is fixedly installed at the end of the support frame (636). The support frame (632) is fixedly installed on the upper end of the pressing barrel (65) near the third motor (633). The end of the support block (637) is inserted into the inner side of the support frame (632).

2. The production method for extracting high-purity mogroside V from monk fruit according to claim 1, characterized in that: In step four, chitosan-benzene composite clarifying agent is added at a ratio of 0.08%-0.12% of the mixture mass, wherein the mass ratio of chitosan to bentonite is 1:

3. The mixture is stirred at an environment of 30-50℃.

3. The production method for extracting high-purity mogroside V from monk fruit according to claim 2, characterized in that: In step five, the simulated moving bed chromatography system consists of 12 chromatographic columns packed with anion exchange resin packing material. The eluent is set to a gradient of 0-35% ethanol aqueous solution, and the feed flow rate is controlled at 2BV / h-4BV / h, while the elution flow rate is 1BV / h-2BV / h.

4. The production method for extracting high-purity mogroside V from monk fruit according to claim 3, characterized in that: In step six, the nanofiltration membrane used in the nanofiltration membrane system has a molecular weight cutoff of 500-1000 Da. During operation, the pressure is controlled at 1 MPa-2 MPa and the temperature at 35℃-45℃.

5. A method for extracting high-purity mogroside V from monk fruit according to claim 4, characterized in that: In step seven, the inlet air temperature of the spray drying tower is set to 175-200℃, and the outlet air temperature is 70-95℃. When using high performance liquid chromatography for detection, the moisture and ash content of the finished product are also tested. The moisture content is required to be ≤5%, and the ash content is required to be ≤0.5%. After all indicators meet the food additive standards, the product is packaged and stored.

6. The production method for extracting high-purity mogroside V from monk fruit according to claim 1, characterized in that: The overall top view shape of the slide plate (4) is arc-shaped, the top view shape of the slide plate (4) and the annular groove (5) are concentric circles, and the overall cross-sectional shape of the slide plate (4) and the cross-sectional shape of the internal cavity of the annular groove (5) are both convex.

7. A method for extracting high-purity mogroside V from monk fruit according to claim 6, characterized in that: A conical lower pressing platform (67) is fixedly installed in the middle of the bottom of the pressing barrel (65). Drainage holes (68) are arranged in a ring at equal intervals on the outer side of the bottom of the pressing barrel (65). The drainage holes (68) penetrate the pressing barrel (65). Reinforcing frames (69) are fixedly installed at both ends of the bottom of the mounting frame (62). The bottom of the reinforcing frame (69) is fixedly connected to the top two sides of the fixing plate (32).

8. A method for extracting high-purity mogroside V from monk fruit according to claim 7, characterized in that: The pressing mechanism (7) includes a fixed arm (71), which is fixedly installed on the upper back of the collection tank (1). A fixed seat (72) is fixedly installed at the front end of the fixed arm (71). A hydraulic cylinder (73) is fixedly installed at the top of the fixed seat (72). A pressing head (74) is fixedly installed through the fixed seat (72) at the output end of the hydraulic cylinder (73). A conical groove (75) is opened at the bottom of the pressing head (74). The top of the conical lower pressing platform (67) is inserted into the conical groove (75) and is in close contact with the inner wall of the conical groove (75).

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