Excitation light source device

Abstract

The invention discloses an excitation light source device and relates to the technical field of biological detection.The excitation light source device comprises an excitation light source module and a window assembly, the excitation light source module is used for emitting excitation light beams to an object to be detected, the window assembly is arranged on one side of the excitation light source module, and the optical axis direction of the excitation light source module intersects with the optical axis direction of the window assembly; a reflected light beam which is emitted from the to-be-measured object and is obtained by reflecting the excitation light beam by the to-be-measured object passes through the window assembly and then is emitted to the other side of the excitation light source module; according to the excitation light source device provided by the invention, the excitation light beam directly reflected by the to-be-detected object and the fluorescence light beam emitted after the sample reaction unit of the to-be-detected object absorbs the excitation light beam can be obtained on the other side of the excitation light source module without using a dichroscope and are used as the reflection light beam; in a to-be-detected object image generated based on the reflected light beam subsequently, the contrast ratio and the signal-to-noise ratio between the sample reaction unit and the background of the to-be-detected object are improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological detection, in particular to an excitation light source device. BACKGROUND

[0002] Whether it is a digital polymerase chain reaction (DPCR) technology or a real-time quantitative PCR (RQPCR) technology, an excitation light source device needs to be used to irradiate a microfluidic chip loaded with a sample reaction unit, and an imaging device (such as a CCD camera) is used to obtain a reflected light beam reflected by the sample reaction unit after the sample reaction unit absorbs an excitation light beam emitted by the excitation light source device, so as to determine an optical signal value (i.e., a fluorescence signal value) of the sample reaction unit.

[0003] In the related art, a dichroic mirror needs to be arranged in the light emission direction of the excitation light source inside the excitation light source device, and the excitation light beam emitted from the excitation light source is refracted to the measured object area by the dichroic mirror, so that the measured object (i.e., the microfluidic chip) is illuminated, the measured object reflects the excitation light beam incident on the measured object to obtain a reflected light beam, and then the reflected light beam is transmitted to the outside of the excitation light source device through the dichroic mirror, and the imaging device receives the reflected light beam to generate a measured object image.

[0004] However, in the process of passing through the dichroic mirror to be incident on the measured object, part of the excitation light beam is lost on the dichroic mirror, and the reflected light beam obtained by the imaging device finally includes not only the fluorescence light beam obtained by the dichroic mirror refracting to the measured object and then the measured object absorbing the excitation light beam as the reflected light beam, but also the excitation light beam directly reflected by the dichroic mirror as the reflected light beam, so that the measured object image generated by the imaging device contains the over-bright background stray light caused by the excitation light beam as the reflected light beam, which affects the accuracy of determining the fluorescence signal value of the sample reaction unit. SUMMARY

[0005] To solve the problem that the excitation light source device in the related art causes the background stray light of the measured object image to be too bright when irradiating the measured object based on the dichroic mirror, the present application provides an excitation light source device.

[0006] To achieve the above-mentioned purpose:

[0007] An embodiment of the present application provides an excitation light source device, which comprises:

[0008] An excitation light source module, which is configured to emit an excitation light beam to a measured object;

[0009] a window assembly disposed on one side of the excitation light source module, an optical axis direction of the excitation light source module intersects with an optical axis direction of the window assembly, and a reflected light beam emitted from the object after passing through the window assembly is emitted to the other side of the excitation light source module, the reflected light beam being obtained by the excitation light beam reflected by the object and a fluorescent light beam emitted after the excitation light beam is absorbed by the object.

[0010] In an embodiment, two excitation light source modules are located on opposite sides of the window assembly, a first included angle between the optical axis direction of one of the two excitation light source modules and the optical axis direction of the window assembly is an acute angle, and a second included angle between the optical axis direction of the other of the two excitation light source modules and the optical axis direction of the window assembly is an acute angle.

[0011] In an embodiment, a first distance between the first optical axis center of one of the two excitation light source modules and the second optical axis center of the window assembly is the same as a second distance between the third optical axis center of the other of the two excitation light source modules and the second optical axis center.

[0012] The first included angle has the same angle size as the second included angle.

[0013] In an embodiment, in a top view of the excitation light source device, the first optical axis center, the second optical axis center, and the third optical axis center are on the same straight line.

[0014] In an embodiment, the excitation light source module comprises:

[0015] a carrier;

[0016] a plurality of excitation light source components disposed on the carrier, the excitation light beams of any two of the excitation light source components having different light wavelength ranges; and

[0017] any of the excitation light source components in the working position of the excitation light source module emits the excitation light beam.

[0018] In an embodiment, the excitation light source module further comprises a plurality of light adjusting components, the plurality of light adjusting components are connected to the plurality of excitation light source components one by one, each of the light adjusting components is disposed on the light emitting side of a corresponding excitation light source component, the excitation light beam passes through the corresponding light adjusting component and is emitted to the object, and the light adjusting component is used to adjust the size of the light spot formed by the excitation light beam incident on the object and the uniformity of the light intensity.

[0019] In an embodiment, the excitation light source module further comprises a driving member configured to drive the carrier to move so as to drive all the excitation light source members to move and drive the target excitation light source member to emit the excitation light beam to pass through the corresponding light adjusting member and then exit to the object to be tested, wherein the target excitation light source member is the excitation light source member in the working position.

[0020] In an embodiment, the excitation light source module further comprises a light adjusting member located on the light exit side of the excitation light source member, wherein the excitation light beam exits to the object to be tested through the light adjusting member, and the light adjusting member is configured to adjust the size of the light spot formed by the excitation light beam incident on the object to be tested and the uniformity of the light intensity.

[0021] In an embodiment, the excitation light source module further comprises a driving member configured to drive the carrier to move so as to drive all the excitation light source members to move relative to the light adjusting member and drive the target excitation light source member to emit the excitation light beam to pass through the light adjusting member and then exit to the object to be tested, wherein the target excitation light source member is the excitation light source member in the working position.

[0022] In an embodiment, the light adjusting member comprises a slide rail extending along a first direction, the excitation light source member further comprises a slide base arranged on the slide rail and connected with the carrier, and the driving member is connected with the carrier and configured to drive the carrier to move so as to drive the slide base to move along the first direction on the slide rail.

[0023] In an embodiment, the light adjusting member comprises:

[0024] a housing configured to form a light beam channel, wherein an entrance of the light beam channel is located at the bottom of the housing, an exit of the light beam channel is located at the top of the housing, one end of the housing close to the entrance is formed with an extension, and the slide rail is arranged on the extension;

[0025] a light adjusting sheet group arranged in the light beam channel, and the excitation light beam exits to the object to be tested after sequentially passing through the entrance, the light adjusting sheet group and the exit.

[0026] In an embodiment, the window assembly comprises:

[0027] a support member provided with a first through hole and a second through hole, wherein the axial direction of the first through hole intersects with the axial direction of the second through hole, the support member is connected with the light adjusting member, at least part of the light adjusting member passes through the first through hole so that the excitation light beam emitted from the light adjusting member exits to the object to be tested through the first through hole; and

[0028] A light-transmitting piece is embedded in the second through hole, so that the reflected light beam exits the light-transmitting piece to the other side of the excitation light source module.

[0029] In an embodiment, the support comprises:

[0030] A first support portion, the first through hole being formed in the first support portion;

[0031] A second support portion on one side of the first support portion, the second support portion being arranged obliquely relative to the first support portion, and the second through hole being formed in the second support portion; wherein,

[0032] The optical axis direction of the light-adjusting piece is perpendicular to the radial direction of the first through hole, and the optical axis direction of the light-transmitting piece is perpendicular to the radial direction of the second through hole.

[0033] The excitation light source device provided by the present application has the following advantages. The window assembly is arranged on one side of the excitation light source piece 12, and the optical axis direction of the excitation light source module intersects the optical axis direction of the window assembly, so that the light exit surface of the excitation light source module is arranged obliquely relative to the light entrance surface of the window assembly. The light path of the excitation light beam incident to the object to be measured is separated from the light path of the reflected light beam exiting to the other side of the excitation light source module. In this way, the excitation light source module directly emits the excitation light beam to the object to be measured. Then, the reflected light beam is obtained from the fluorescence light beam emitted after the excitation light beam is absorbed by the sample reaction unit of the object to be measured and the excitation light beam reflected by the object to be measured. Finally, the reflected light beam exiting from the object to be measured exits to the other side of the excitation light source module after passing through the window assembly. Therefore, the reflected light beam can be obtained on the other side of the excitation light source module without using a dichroic mirror in the excitation light source device. The light interference caused by the dichroic mirror is avoided. The contrast between the sample reaction unit and the background of the object to be measured in the image of the object to be measured generated based on the reflected light beam is improved, so that the signal-to-noise ratio is improved, and the accuracy of determining the fluorescence signal value of the sample reaction unit is improved. BRIEF DESCRIPTION OF DRAWINGS

[0034] Figure 1 The block diagram of the excitation light source device and the object to be measured provided by an embodiment of the present application is shown in the figure.

[0035] Figure 2 The block diagram of the excitation light source device and the object to be measured provided by another embodiment of the present application is shown in the figure.

[0036] Figure 3 The block diagram of the excitation light source device and the object to be measured provided by another embodiment of the present application is shown in the figure. Figure 2 The top view of the excitation light source device and the object to be measured is shown in the figure.

[0037] Figure 4 The top view of the excitation light source device and the object to be measured is shown in the figure. Figure 1 Or Figure 2A structural schematic diagram of the excitation light source module shown in

[0038] Figure 5 A structural schematic diagram of the excitation light source module shown in Figure 4 A sectional view of the excitation light source module shown in the A-A' direction;

[0039] Figure 6 A structural schematic diagram of the excitation light source module shown in Figure 1 A structural schematic diagram of the excitation light source module shown in Figure 2 A structural schematic diagram of the excitation light source module shown in

[0040] Figure 7 A structural schematic diagram of the excitation light source module shown in Figure 6 A sectional view of the excitation light source module shown in the B-B' direction;

[0041] Figure 8 A structural schematic diagram of the excitation light source module shown in Figure 6 A structural schematic diagram of the excitation light source module shown in

[0042] Figure 9 A structural schematic diagram of the excitation light source module shown in Figure 1 A structural schematic diagram of the excitation light source module shown in Figure 2 A structural schematic diagram of the excitation light source module shown in

[0043] Figure 10 A structural schematic diagram of the excitation light source module shown in Figure 1 A structural schematic diagram of the excitation light source module shown in Figure 2 A structural schematic diagram of the excitation light source module shown in

[0044] Figure 11 A structural schematic diagram of the excitation light source module shown in Figure 2 A structural schematic diagram of the excitation light source module shown in

[0045] Figure 12 A structural schematic diagram of the excitation light source module shown in Figure 11 A structural schematic diagram of the excitation light source module shown in

[0046] Figure 13 A structural schematic diagram of the excitation light source module shown in Figure 11 A sectional view of the excitation light source module shown in the C-C' direction;

[0047] Figure 14 A structural schematic diagram of the excitation light source module shown in Figure 2 A structural schematic diagram of the excitation light source module shown in DETAILED DESCRIPTION

[0048] The application will be described in further detail below with specific reference to the drawings. Like elements in different embodiments are denoted by like reference numerals. In the following description, numerous specific details are described to provide a thorough understanding of the application. However, it will be apparent to one skilled in the art that the application can be practiced without these specific details. In other instances, well-known structures have not been shown or described in detail to avoid obscuring aspects of the application. The detailed description is presented in terms of specific embodiments with reference to the accompanying drawings.

[0049] In addition, features, operations, or characteristics described in the specification can be combined in any suitable manner in various embodiments. Also, various steps or acts in a method described can be performed in a different order from the one suggested in this specification, or can be performed concurrently. Thus, the order in which steps or acts are described is not necessarily the order in which they are performed. The various embodiments described herein are not necessarily mutually exclusive, and can be combined in any suitable manner.

[0050] The serial numbers of components in this specification, such as "first", "second", etc., are used only to distinguish the described objects, and do not have any sequential or technical meaning. The "connection" in this specification includes direct and indirect connections unless otherwise specified.

[0051] In an embodiment provided by the application, as shown in Figure 1 The excitation light source device 100 includes an excitation light source module 10 and a window assembly 20.

[0052] The excitation light source module 10 and the window assembly 20 are on the same side of the preset position w1. Exemplarily, a microfluidic chip to be detected (hereinafter referred to as a sample 200) is arranged at the preset position w1.

[0053] The excitation light source module 10 is configured to emit an excitation light beam L1 to the sample 200, i.e., the excitation light source module 10 directly emits the excitation light beam L1 to the sample 200 without using a dichroic mirror to refract the excitation light beam L1 to the sample 200.

[0054] The window assembly 20 is arranged on one side of the excitation light source module 10, and the optical axis direction d1 of the excitation light source module 10 intersects the optical axis direction d2 of the window assembly 20. The reflected light beam L2 emitted from the sample 200 exits to the other side of the excitation light source module 10 after passing through the window assembly 20. The reflected light beam L2 is obtained by reflecting the excitation light beam L1 by the sample 200.

[0055] Since the optical axis direction d1 of the excitation light source module 10 intersects the optical axis direction d2 of the window assembly 20, the light-emitting surface of the excitation light source module 10 is tilted relative to the light-incident surface of the window assembly 20. As a result, the light path of the excitation beam L1 incident on the test object 200 and the light path of the reflected beam L2 exiting through the window assembly 20 to the other side of the excitation light source module 10 are not coincident, but separate.

[0056] Since the optical path of the excitation beam L1 incident on the test object 200 is separated from the optical path of the window assembly 20 exiting to the other side of the excitation light source module 10, the excitation beam L1 and the reflected beam L2 do not interfere with each other. Therefore, the beam obtained on the other side of the excitation light source module 10 will only be the reflected beam L2 obtained by the test object 200 reflecting the excitation beam L1. It is worth mentioning that, in any embodiment of this application, the reflected beam L2 includes the excitation beam directly reflected by the test object and the fluorescence beam emitted by the sample reaction unit of the test object after absorbing the excitation beam.

[0057] By eliminating the need for a dichroic mirror to avoid light interference from the image of the test object, the contrast between the sample reaction unit and the background of the test object 200 is improved in the subsequent generation of the test object image based on the reflected beam L2 obtained on the other side of the excitation light source module 10. This improves the signal-to-noise ratio of the test object image and enhances the accuracy of determining the fluorescence signal value of the sample reaction unit.

[0058] Furthermore, in another embodiment, such as Figure 2 As shown, the excitation light source device 100 includes two excitation light source modules 10. The two excitation light source modules 10 are located on opposite sides of the window assembly 20.

[0059] One of the two excitation light source modules 10 ( Figure 2 The first angle a1 between the optical axis of the first excitation light source module 10a) and the optical axis d2 of the window assembly is an acute angle.

[0060] Of the two excitation light source modules 10, the other one is ( Figure 2 The second angle a2 between the optical axis of the second excitation light source module 10b and the optical axis d2 of the window assembly shown is an acute angle.

[0061] exist Figure 1In the shown excitation light source device 100, due to the inclined arrangement of the light exit surface of the excitation light source module 10 relative to the light entrance surface of the window assembly 20, there is an optical path difference between each excitation light ray in the excitation light beam L1 incident to the object 200 at the preset position w1, and thus the spot formed by the excitation light beam L1 of the single excitation light source module 10 on the object 200 presents a gradually changing bright-dark unevenness according to the near-far of the optical path, resulting in the problem of uneven brightness in the image of the object 200 obtained according to the excitation light beam L1.

[0062] Based on this, the inventors of the present application further propose to arrange the excitation light source modules 10 on opposite sides of the window assembly 20, and to make the included angle between the optical axis direction of each of the two excitation light source modules 10 and the optical axis direction d2 of the window assembly an acute angle, so as to ensure that at least part of the excitation light beam L1 emitted by each excitation light source module 10 can be incident to the object 200, thereby utilizing the spot formed by the excitation light beam L1 of the single excitation light source module 10 on the object 200 to present a gradually changing bright-dark unevenness according to the near-far of the optical path, so as to improve the bright-dark uniformity of the spot formed by the superposition of the excitation light beams L1 of the two excitation light source modules 10 on the object 200.

[0063] Specifically, as shown in Figure 2 from left to right, the bright degree of the first spot formed by the excitation light beam L1 emitted by the excitation light source module 10 on the left side of the window assembly 20 on the object 200 decreases. And along the direction from right to left, the bright degree of the second spot formed by the excitation light beam L1 emitted by the excitation light source module 10 on the right side of the window assembly 20 on the object 200 decreases. And the overall brightness uniformity of the equivalent spot formed by the superposition of the first spot and the second spot is effectively improved.

[0064] Further, please continue to refer to Figure 2 In an embodiment, the first distance D1 between the first optical axis center o1 of one of the two excitation light source modules 10 (the first excitation light source module 10a in Figure 2 ) and the second optical axis center o2 of the window assembly 20 is the same as the second distance D2 between the third optical axis center o3 of the other of the two excitation light source modules 10 (the second excitation light source module 10b in Figure 2 ) and the second optical axis center of the window assembly 20. And the angle size of the first included angle a1 is the same as the angle size of the second included angle a2.

[0065] In this way, the optical path length of the excitation beam L1 of the first excitation light source module 10a incident on the test object 200 is complementary to that of the excitation beam L1 of the second excitation light source module 10b incident on the test object 200. Under the condition that the light intensity of the excitation beam L1 provided by the two excitation light source modules 10 is constant, the brightness uniformity of the equivalent spot obtained by superimposing the two spots is further improved.

[0066] Furthermore, in one embodiment, combined with Figure 2 as well as Figure 3 As shown, in the top view of the excitation light source device 100 (i.e., from...) Figure 2 (Looking from "up" to "down"), the first optical axis center o1, the second optical axis center o2, and the third optical axis center o3 are on the same straight line. In this way, when the preset position w1 is in the axial direction of the window assembly 20, the optical path of the excitation beam of the first excitation light source 12 incident on the test object 200 is the shortest, and the optical path of the excitation beam of the second excitation light source 12 incident on the test object 200 is also the shortest. In this way, the brightness uniformity of the equivalent light spot obtained by superimposing the two light spots is almost or completely consistent.

[0067] Specifically, in one embodiment, such as Figure 4 as well as Figure 5 As shown, the excitation light source module 10 includes a carrier 11 and a plurality of excitation light source elements 12 disposed on the carrier 11. The excitation beams of any two excitation light source elements 12 have different wavelength ranges, and any excitation light source element 12 located at the working position w2 of the excitation light source module 10 emits an excitation beam.

[0068] It is worth mentioning that each excitation light source 12 includes one or more excitation light beads 121.

[0069] Furthermore, in one embodiment, each excitation light source 12, in addition to the excitation lamp bead 121, also sequentially includes a converging lens group 122 and a filter 123. For example, as shown... Figure 5 As shown, a converging lens group 122 is provided between the excitation lamp bead 121 and the filter 123. The converging lens group 122 includes at least two converging lenses. The purpose of providing at least two converging lenses is to minimize the divergence angle of the excitation beam and improve the light utilization rate of the excitation beam.

[0070] It is worth mentioning that by placing the filter 123 on the side of the converging lens group 122 away from the excitation light bead 121, the filter 123 allows the excitation light beam within a specific wavelength range to pass through, and reflects or absorbs the excitation light beam outside the specific wavelength range, thereby achieving selective control of the wavelength of the excitation light beam emitted from the excitation light source device 100.

[0071] It is worth mentioning that, such asFigure 4 and Figure 5 As shown in FIG. 1, the excitation light source module 10 further comprises a heat dissipation member 16 arranged on the carrier 11, so as to dissipate heat of the excitation light source member 12 emitting the excitation light beam.

[0072] In the excitation light source module 10 provided by the embodiment, by arranging the excitation light source member 12 providing the excitation light beam L1 of different wavelength ranges, and adjusting the relative position relationship between the plurality of excitation light source members 12 and the working position w2, the excitation light beam L1 of a specific wavelength range can be selected to exit to the object 200 to be detected, so that the object 200 can be detected in different spectral channels.

[0073] Further, in an embodiment, as shown in FIG. 1, the excitation light source module 10 further comprises a light adjusting member 13. The light adjusting member 13 is arranged on the light exit side of the excitation light source member 12, and the excitation light beam L1 exits to the object 200 to be detected through the light adjusting member 13. The light adjusting member 13 is used to adjust the size of the light spot formed by the excitation light beam L1 incident to the object 200 to be detected after exiting from the excitation light source member 12 located at the working position w2, and the uniformity of the light intensity. Figure 6 and Figure 7 That is to say, in the excitation light source module 10 provided by the embodiment, by adjusting the relative position relationship between the excitation light source member 12 and the light adjusting member 13, it is ensured that the size of the light spot formed by the excitation light beam L1 emitted by any excitation light source member 12 located at the working position w2 on the object 200 to be detected can cover the detection area of the object 200 to be detected, and it is ensured that the uniformity of the light intensity of the light spot formed by the excitation light beam L1 emitted by any excitation light source member 12 located at the working position w2 on the object 200 to be detected, so that the subsequent imaging device can generate a complete and clear image of the object 200 to be detected based on the reflected light beam L2.

[0074] The excitation light source module 10 of the embodiment only needs to be provided with one light adjusting member 13. Since the number of light adjusting members 13 is reduced, the overall size of the excitation light source module 10 can be further miniaturized, so that the overall size of the excitation light source device 100 can be further miniaturized, and the space occupied by the excitation light source device 100 can be reduced.

[0075] Further, as shown in FIG. 1, in an embodiment, the excitation light source module 10 further comprises a driving member 14. The driving member 14 is used to drive the carrier 11 to move, so as to drive all the excitation light source members 12 to move relative to the light adjusting member 13, and drive the excitation light beam L1 emitted by the target excitation light source member 12 to exit to the object 200 to be detected through the light adjusting member 13. The target excitation light source member 12 is the excitation light source member 12 located at the working position w2.

[0076] Further, as shown in FIG. 1, in an embodiment, the excitation light source module 10 further comprises a driving member 14. The driving member 14 is used to drive the carrier 11 to move, so as to drive all the excitation light source members 12 to move relative to the light adjusting member 13, and drive the excitation light beam L1 emitted by the target excitation light source member 12 to exit to the object 200 to be detected through the light adjusting member 13. The target excitation light source member 12 is the excitation light source member 12 located at the working position w2. Figure 7 to Figure 9 , Figure 12

[0077] ​In the excitation light source device 100 provided in the embodiment, the driving member 14 is arranged to integrally control the movement of the excitation light source member 12 and the light adjusting member 13 relative to the working position w2, thereby eliminating the need for manually adjusting the excitation light source module 10.

[0078] It is worth mentioning that in another embodiment provided in the present application, the excitation light source module 10 can also include a plurality of light adjusting members 13. The plurality of light adjusting members 13 are connected one by one with the plurality of excitation light source members 12, and each light adjusting member 13 is arranged on the light emitting side of the corresponding excitation light source member 12. The excitation light beam L1 emitted by the excitation light source member 12 at the working position w2 is emitted to the object 200 again through the light adjusting member 13. Among them, each light adjusting member 13 is used to adjust the size and light intensity uniformity of the light spot formed by the excitation light beam L1 incident on the object 200.

[0079] In the excitation light source module 10 provided in the embodiment, by arranging the corresponding light adjusting member 13 for each excitation light source member 12, it is ensured that the size of the light spot formed by the excitation light beam L1 emitted by any excitation light source member 12 on the object 200 can cover the detection area of the object 200, and the light intensity uniformity of the light spot formed by the excitation light beam L1 emitted by any excitation light source member 12 on the object 200 is ensured, so that a complete and clear image of the object 200 can be generated based on the reflected light beam L2 subsequently.

[0080] Compared with the excitation light source module 10 shown in Figure 6 and Figure 7 , in the excitation light source module 10 provided in the embodiment, there is a corresponding light adjusting member 13 for each excitation light source member 12. Therefore, during the adjustment of the excitation light source member 12 at the working position w2, the alignment relationship between the excitation light source member 12 and the light adjusting member 13 does not need to be adjusted, thereby eliminating the need for registration and alignment during switching of the excitation light source member 12, and saving detection time.

[0081] Further, the excitation light source module 10 further includes a driving member 14. The driving member 14 is used to drive the carrier 11 to move, so that the carrier 11 drives all the excitation light source members 12 to move, and the excitation light beam L1 emitted by the target excitation light source member 12 passes through the corresponding light adjusting member 13 and is emitted to the object 200. Among them, the target excitation light source member 12 is the excitation light source member 12 at the working position w2. Since each excitation light source member 12 is connected with a light adjusting member 13, when the carrier 11 drives all the excitation light source members 12 to move, each excitation light source member 12 drives the connected light adjusting member 13 to move synchronously.

[0082] Further, please continue to refer to Figure 8 and Figure 9In an embodiment, the light adjusting piece 13 comprises a sliding rail 135 extending along a first direction d3, and the excitation light source module 10 further comprises a sliding seat 15 arranged on the sliding rail 135.

[0083] The sliding rail 135 extends along the first direction d3, and the first direction d3 is the arrangement direction of the plurality of excitation light source pieces 12. It is worth mentioning that the first direction d3 can be a linear extension direction, or the first direction d3 can be an arcuate extension direction. Regardless of whether the first direction d3 is a linear extension direction or an arcuate extension direction, as long as the reflected light beam L2 emitted from the window assembly 20 is not blocked.

[0084] The sliding seat 15 is arranged on the sliding rail 135, and the sliding seat 15 is connected with the carrier 11. That is, under the action of an external force, the sliding seat 15 moves along the first direction d3 on the sliding rail 135, so that the sliding seat 15 drives the carrier 11 to move along the first direction d3, and then the excitation light source piece 12 corresponding to the working position w2 changes accordingly.

[0085] The driving piece 14 is connected with the carrier 11, and the driving piece 14 is used to drive the carrier 11 to move, so that the sliding seat 15 slides along the first direction d3 on the sliding rail 135.

[0086] Specifically, as shown in Figure 9 and Figure 14 , the driving piece 14 comprises a driving motor 141, a traveling rod 142, a guide plate 143, and a push block 144.

[0087] The traveling rod 142 passes through the guide plate 143 along the first direction d3, one end of the traveling rod 142 is connected with the driving motor 141, and the other end of the traveling rod 142 is connected with the push block 144. The push block 144 is arranged on the guide plate 143, and the push block 144 is fixedly connected with the carrier 11.

[0088] The driving motor 141 converts electric energy into kinetic energy to drive the traveling rod 142 to move along the first direction d3, so that the traveling rod 142 drives the push block 144 to move along the first direction d3 on the guide plate 143, and then the push block 144 drives the carrier 11 to move along the first direction d3.

[0089] Specifically, in an embodiment provided by the present application, as shown in Figure 6 and Figure 7 , the light adjusting piece 13 further comprises a housing 131 and a light adjusting sheet group 133.

[0090] The shell 131 is configured to have a light beam passage 1311, an entrance 1311a of the light beam passage 1311 is located at the bottom of the shell 131, and an exit 1311b of the light beam passage 1311 is located at the top of the shell 131. The bottom of the shell 131 and the top of the shell 131 are oppositely arranged. In addition, the shell 131 is formed with an extension 1312 at one end close to the entrance 1311a of the light beam passage 1311, and the slide rail 135 is arranged on the extension 1312.

[0091] In the excitation light source device 100 provided in the embodiment, by forming the extension 1312 at one end of the shell 131 close to the entrance 1311a of the light beam passage 1311 of the light adjusting piece 13, and arranging the slide rail 135 on the extension 1312, the light adjusting piece 13, the excitation light source piece 12, the carrier piece 11, and the driving piece 14 can be integrated and assembled in one body, and the excitation light beam L1 emitted by the excitation light source piece 12 can be emitted to the object 200 to be measured through the light beam passage 1311 of the shell 131.

[0092] The light adjusting piece group 133 is arranged in the light beam passage 1311, and the excitation light beam L1 is emitted to the object 200 to be measured after sequentially passing through the entrance 1311a of the light beam passage 1311, the light adjusting piece group 133, and the exit 1311b of the light beam passage 1311. The light adjusting piece group 133 has the functions of uniform light and adjusting focus for the excitation light beam L1.

[0093] Specifically, as shown in Figure 7 The light adjusting piece group 133 includes at least one microarray lens 1332, at least one focusing lens 1333, and at least one collimating lens 1331. The microarray lens 1332 performs uniform light processing on the excitation light beam L1, so that the light spot formed by the excitation light beam on the object 200 to be measured is uniform in brightness. The focusing lens 1333 performs focusing processing on the excitation light beam L1, so as to adjust the size of the light spot formed by the excitation light beam on the object 200 to be measured. The collimating lens 1331 performs collimation processing on the excitation light beam, so as to adjust the exit angle of each excitation light line of the excitation light beam to be parallel to the optical axis of the collimating lens 1331.

[0094] Specifically, as shown in Figure 10 The window assembly 20 includes a support 21 and a light-transmitting piece 22.

[0095] The support 21 is provided with a first through hole 2111 and a second through hole 2131. The axial direction of the first through hole 2111 intersects with the axial direction of the second through hole 2131. In addition, the support 21 is connected with the light adjusting piece 13, and at least part of the light adjusting piece 13 passes through the first through hole 2111, so that the excitation light beam L1 emitted from the light adjusting piece 13 is emitted to the object 200 to be measured through the first through hole 2111.

[0096] It is worth mentioning that when the support is provided with two first through holes 2111, the axial direction of each first through hole 2111 intersects with the axial direction of the second through hole 2131.

[0097] The light-transmitting piece 22 is embedded in the second through hole 2131, so that the reflected light beam L2 emitted from the to-be-measured object 200 is emitted to the other side of the excitation light source module 10 through the light-transmitting piece 22. In the present example, the light-transmitting piece 22 can be specifically a planar transparent glass lens, so as to ensure that the reflected light beam L2 emitted from the to-be-measured object 200 is emitted to the other side of the excitation light source module 10 as much as possible.

[0098] In the excitation light source device 100 provided in the present embodiment, the first through hole 2111 is formed on the support 21 of the window assembly 20, and the light-adjusting piece 13 of the light filter module is connected to the support 21 through the first through hole 2111, so as to integrate the excitation light source module 10 and the window assembly 20, make the assembly between the excitation light source module 10 and the window assembly 20 more compact, reduce the space occupied by the excitation light source device 100 as a whole, and realize the small size of the excitation light source device 100.

[0099] Further, as shown in Figure 10 to Figure 14 The support 21 includes a first support portion 211 and a second support portion 213 located on one side of the first support portion 211, and the second support portion 213 is arranged obliquely relative to the first support portion 211.

[0100] The first through hole 2111 is formed in the first support portion 211, and the optical axis direction of the light-adjusting piece 13 is perpendicular to the radial direction d4 of the first through hole. It is worth mentioning that in the present embodiment, the optical axis direction of the light-adjusting piece 13 coincides with the optical axis direction of the excitation light source piece 12 in the working position, in other words, the optical axis direction of the light-adjusting piece 13 is the optical axis direction d1 of the excitation light source module 10.

[0101] The second through hole 2131 is formed in the second support portion 213, and the optical axis direction of the light-transmitting piece 22 is perpendicular to the radial direction d5 of the second through hole. It is worth mentioning that in the present embodiment, the optical axis direction of the light-transmitting piece 22 coincides with the optical axis direction of the window assembly, in other words, the optical axis direction of the light-transmitting piece 22 is the optical axis direction d2 of the window assembly 20.

[0102] In the embodiment, by controlling the assembly relationship between the optical axis direction of the light adjusting piece 13 and the radial direction d4 of the first through hole, and the assembly relationship between the optical axis direction of the light transmitting piece 22 and the radial direction d5 of the second through hole, respectively, the optical axis direction of the light adjusting piece 13 (i.e. the optical axis direction d1 of the excitation light source module 10) and the optical axis direction of the light transmitting piece 22 can be determined to intersect. In addition, based on the design of the support piece 21 of the embodiment, on the basis of facilitating the assembly of the excitation light source device 100, the relative position relationship between the excitation light source module 10 and the window assembly 20 does not need to be additionally debugged, the optical axis direction d1 of the excitation light source module 10 and the optical axis direction d2 of the window assembly 20 can be intersected, the angle formed by the intersection is a fixed value, the assembly efficiency is improved, and the batch production of the excitation light source device 100 is facilitated.

[0103] The technical features of the above-described embodiments can be combined in any manner. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described, but as long as the combinations of the technical features do not contradict, they should be considered within the scope of the present disclosure.

[0104] The above description is merely specific embodiments of the present application, but the protection scope of the present application is not limited thereto. Any person skilled in the art can easily think of changes or replacements within the technical scope disclosed by the present application, which should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. An excitation light source device, characterized by comprising: The excitation light source module is used for emitting an excitation light beam to a to-be-tested object. The window assembly is arranged on one side of the excitation light source module, and the optical axis direction of the excitation light source module intersects with the optical axis direction of the window assembly. The reflected light beam emitted from the to-be-tested object passes through the window assembly and is then emitted to the other side of the excitation light source module. The reflected light beam is obtained by the to-be-tested object reflecting the excitation light beam and the fluorescent light beam emitted after the to-be-tested object absorbs the excitation light beam. The first included angle between the optical axis direction of one of the two excitation light source modules and the optical axis direction of the window assembly is an acute angle, and the second included angle between the optical axis direction of the other of the two excitation light source modules and the optical axis direction of the window assembly is an acute angle.

2. The excitation light source device according to claim 1, wherein 3. The excitation light source device according to claim 2, wherein The first distance between the first optical axis center of one of the two excitation light source modules and the second optical axis center of the window assembly is the same as the second distance between the third optical axis center of the other of the two excitation light source modules and the second optical axis center. The angle of the first included angle is the same as the angle of the second included angle. In the top view of the excitation light source device, the first optical axis center, the second optical axis center and the third optical axis center are on the same straight line.

4. The excitation light source device according to claim 3, wherein The excitation light source module comprises:

5. The excitation light source apparatus according to claim 1, wherein a carrier; a plurality of excitation light source pieces arranged on the carrier, and the light wavelengths of the excitation light beams of any two excitation light source pieces are different; wherein Any excitation light source piece in the working position of the excitation light source module emits the excitation light beam. The excitation light source module further comprises a plurality of light adjusting pieces, and each light adjusting piece is arranged on the light emitting side of a corresponding excitation light source piece. The excitation light beam passes through the corresponding light adjusting piece and is then emitted to the to-be-tested object. The light adjusting piece is used to adjust the size of the light spot formed by the excitation light beam incident on the to-be-tested object and the light intensity uniformity.

6. The excitation light source device according to claim 5, wherein The excitation light source module further comprises a driving piece. The driving piece is used to drive the carrier to move, so that the carrier drives all the excitation light source pieces to move, and the excitation light beam emitted by the target excitation light source piece passes through the corresponding light adjusting piece and is then emitted to the to-be-tested object. The target excitation light source piece is the excitation light source piece in the working position.

7. The excitation light source device according to claim 6, wherein The excitation light source module further comprises a light adjusting piece arranged on the light emitting side of the excitation light source piece. The excitation light beam passes through the light adjusting piece and is then emitted to the to-be-tested object. The light adjusting piece is used to adjust the size of the light spot formed by the excitation light beam incident on the to-be-tested object and the light intensity uniformity.

8. The excitation light source apparatus according to claim 5, wherein ​ 9. The excitation light source device according to claim 8, wherein The excitation light source module further comprises a driving member configured to drive the carrier to move so as to drive all the excitation light source members to move relative to the light adjusting member, and drive the target excitation light source member to emit the excitation light beam to pass through the light adjusting member and then be emitted to the object to be tested.

10. The excitation light source device according to claim 9, wherein The light adjusting member comprises a slide rail extending along a first direction, and the excitation light source module further comprises a slide base arranged on the slide rail and connected with the carrier, and the driving member is connected with the carrier and configured to drive the carrier to move so as to drive the slide base to move along the first direction on the slide rail.

11. The excitation light source device according to claim 10, wherein The light adjusting member further comprises: a housing configured to form a light beam channel, an entrance of the light beam channel being located at a bottom of the housing, and an exit of the light beam channel being located at a top of the housing, wherein an extension is formed at one end of the housing close to the entrance, and the slide rail is arranged on the extension; a light adjusting sheet group arranged in the light beam channel, and the excitation light beam is emitted to the object to be tested after sequentially passing through the entrance, the light adjusting sheet group and the exit.

12. The excitation light source device according to claim 8, wherein The window assembly comprises: a support member provided with a first through hole and a second through hole, an axial direction of the first through hole intersecting an axial direction of the second through hole, the support member being connected with the light adjusting member, and at least part of the light adjusting member passing through the first through hole so that the excitation light beam emitted from the light adjusting member is emitted to the object to be tested through the first through hole; a light-transmitting member embedded in the second through hole so that the reflected light beam is emitted to the other side of the excitation light source module through the light-transmitting member.

13. The excitation light source device according to claim 12, wherein The support member comprises: a first support portion, the first through hole being arranged in the first support portion; a second support portion arranged on one side of the first support portion and inclined relative to the first support portion, the second through hole being arranged in the second support portion; and an optical axis direction of the light adjusting member being perpendicular to a radial direction of the first through hole, and an optical axis direction of the light-transmitting member being perpendicular to a radial direction of the second through hole.

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