Spore solid bacterium inoculation method

By using spore-based solid-spore inoculation, the inoculation process for edible fungi is simplified, hardware and environmental requirements are reduced, and the success rate of infection and economic benefits are improved, thus solving the problems of high cost and low efficiency in existing technologies.

CN121241849APending Publication Date: 2026-01-02DUNHUA NORTHERN BIOLOGICAL CO LTD
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Patent Information

Application Number
CN202511416980.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-09-30
Publication Date
2026-01-02

AI Technical Summary

Technical Problem

Existing methods of inoculating edible fungi increase the workload of workers, affect work efficiency, and have long production cycles, resulting in high costs and low efficiency.

Method used

A spore solid inoculation method was adopted, including the preparation of culture media, the separation of spore source culture media and recipient culture media, spore induction and environmental parameter control. The spores were allowed to naturally fall onto the recipient culture medium for infection. Combined with scratch treatment and environmental control, the spore attachment area and infection success rate were improved.

Benefits of technology

It reduces the stringent requirements for hardware facilities and environmental conditions, simplifies operating procedures, lowers production costs, improves work efficiency and infection success rate, and enhances economic benefits.

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Abstract

The invention provides a spore solid bacterium inoculation method. Relates to the technical field of edible mushrooms. The method comprises the following steps: S1, culture medium group preparation: selecting trees and setting a spore source culture medium group and a receptor culture medium group in groups; s2, inducing the spore source culture medium group to form a mature sporocarp, inoculating a target edible mushroom strain on the spore source culture medium group, inducing the strain to grow and forming the mature sporocarp; s3, controlling environmental parameters to promote spores to eject and fall on the receptor culture medium group; s4, the sporocarp is obtained through natural culture, the spore source culture medium group accounts for 5%-20% of the total culture medium group in volume, the surface of the receptor culture medium group in the S1 needs to be subjected to scribing treatment, the depth of a notch is 2-5 mm, the condition that the notch makes contact with the xylem is adopted as the criterion, and if the length of the tilia wood is smaller than 30 cm, scribing is not needed. The spore solid bacterium inoculation method provided by the invention has the advantages of reducing the planting cost and improving the economic benefit.
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