Spore solid bacterium inoculation method
By using spore-based solid-spore inoculation, the inoculation process for edible fungi is simplified, hardware and environmental requirements are reduced, and the success rate of infection and economic benefits are improved, thus solving the problems of high cost and low efficiency in existing technologies.
Patent Information
- Application Number
- CN202511416980.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-30
- Publication Date
- 2026-01-02
AI Technical Summary
Existing methods of inoculating edible fungi increase the workload of workers, affect work efficiency, and have long production cycles, resulting in high costs and low efficiency.
A spore solid inoculation method was adopted, including the preparation of culture media, the separation of spore source culture media and recipient culture media, spore induction and environmental parameter control. The spores were allowed to naturally fall onto the recipient culture medium for infection. Combined with scratch treatment and environmental control, the spore attachment area and infection success rate were improved.
It reduces the stringent requirements for hardware facilities and environmental conditions, simplifies operating procedures, lowers production costs, improves work efficiency and infection success rate, and enhances economic benefits.
Smart Images
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Abstract
Description
Technical Field
[0001] This invention relates to the field of edible fungi technology, and in particular to a method for inoculating solid spore fungi. Background Technology
[0002] Solid fungi are fungi that grow on solid culture media or form solid structures. Edible fungi are a type of solid fungi, which are edible mushrooms that can form large fleshy (or gelatinous) fruiting bodies or sclerotia. Because edible fungi have a broad market prospect, they are widely cultivated and inoculated.
[0003] The current mainstream inoculation method for edible fungi involves first drilling holes in the cultivation wooden stalks, then inserting the fungus into the holes, and finally covering the stuffed holes before planting. This increases the workload of workers and affects work efficiency. In addition, the long production cycle of the fungus greatly extends the overall time span from preparation to harvest.
[0004] Utility Invention Content
[0005] The technical problem solved by this invention is to provide a method for inoculating solid spore bacteria that reduces planting costs and improves economic benefits.
[0006] To solve the above-mentioned technical problems, the present invention provides a method for inoculating solid spore bacteria, comprising the following steps:
[0007] S1. Preparation of culture medium groups: Select trees and divide them into spore source culture medium group and recipient culture medium group;
[0008] S2. Inducing the formation of mature fruiting bodies in the spore source culture medium group: The target edible fungus strain is inoculated onto the spore source culture medium group to induce the strain to grow and form mature fruiting bodies;
[0009] S3. Controlling environmental parameters to induce spores to be ejected and fall onto the recipient culture medium;
[0010] S4. Obtaining fruiting bodies through natural cultivation.
[0011] Preferably, the spore source culture medium accounts for 5%-20% of the total volume of the total culture medium.
[0012] Preferably, when the length of the receptor culture medium group in S1 exceeds 30cm, the surface is subjected to intermittent scratching treatment with a scratching depth of 2-5mm. When the length of the receptor culture medium group does not exceed 30cm, the surface is not subjected to intermittent scratching treatment.
[0013] Preferably, the incision marks are straight lines with a uniform width of 3 mm and a depth of 4 mm to ensure the unobstructed flow of the spore inoculation channel.
[0014] Preferably, after the scoring is completed, the debris is cleaned up using sterile tools and the surface is disinfected with a disinfectant.
[0015] Preferably, the disinfectant is a potassium permanganate solution with a concentration of 0.1%-0.3%.
[0016] Preferably, the air velocity during the spore release period in S2 is maintained at <0.2m / s.
[0017] Compared with related technologies, the spore solid bacteria inoculation method provided by the present invention has the following beneficial effects:
[0018] This application only requires the preparation of a small amount of bacterial strain, without the need for specialized equipment and a strictly sterile environment, greatly reducing the stringent requirements for hardware facilities and environmental conditions, making the inoculation operation more convenient and easy, thereby reducing production costs. It also eliminates the need for manual operations such as removing the inoculum, stuffing the inoculum, and covering the inoculum, greatly reducing the stringent requirements for hardware facilities and environmental conditions, making the inoculation operation more convenient and easy, reducing the workload of workers while improving work efficiency, and also has the advantage of population.
[0019] By scratching the surface of the recipient culture medium to form specific network fissures, the attachment area of spores is increased, while ensuring the unobstructed spore inoculation channel. Combined with strict control of environmental parameters, such as suitable temperature, humidity, light and ventilation conditions, the success rate of spore infection of the recipient culture medium is significantly improved, thereby increasing economic benefits. Detailed Implementation
[0020] Example 1
[0021] This embodiment provides a method for inoculating solid spore culture, including: preparing culture media; selecting multiple oak mycelium bags with a diameter of 10cm and a height of 22cm and dividing them into a spore source culture media group and a recipient culture media group; inducing the spore source culture media group to form mature fruiting bodies, while simultaneously soaking the recipient culture media group to maintain sufficient moisture; cultivating the prepared spore source into substrate mycelium bags, hanging them on top of the recipient culture media group mycelium bags for fruiting management, and maintaining a distance of 0.8m between the spore source culture media group mycelium bags and the recipient culture media group mycelium bags, allowing the spores on the spore source culture media group to naturally fall onto the recipient culture media group mycelium bags under the influence of gravity for inoculation.
[0022] During spore infection, environmental conditions were artificially controlled to maintain a temperature of 18-24℃, humidity of 90%, and a calm environment for 21 days. This ensured that the spores had sufficient time to settle and infect the recipient culture medium. At the same time, changes in environmental parameters were closely monitored and adjusted in a timely manner to ensure the smooth progress of spore infection.
[0023] In the cultivation of wood ear mushrooms, intelligent environmental control algorithms are used to precisely manage the cultivation environment, for example:
[0024]
[0025]
[0026] When the ambient humidity is detected to be below 80%, the micro-spraying system will be automatically activated, or spraying for 3 minutes every 2 hours to increase the ambient humidity and meet the water requirements for the growth of black fungus. When the temperature is above 26℃, the shade net will be opened in time to block some direct sunlight and lower the temperature. At the same time, the ventilation system will be activated to enhance air circulation and remove heat, creating a suitable temperature environment for the growth of black fungus.
[0027] Confirmed by advanced PCR detection technology, the infection success rate of Auricularia auricula-judae spores in the recipient culture medium group of fungal bags in this embodiment was as high as 98.5%. This data fully demonstrates the significant advantage of the method of the present invention in improving the spore infection success rate.
[0028] Example 2
[0029] This embodiment provides a method for inoculating solid spore culture, including: preparing the culture medium group; selecting multiple oak spore bags with a length of 30-50cm and dividing them into a spore source culture medium group and a recipient culture medium group; inducing the spore source culture medium group to form mature fruiting bodies, while simultaneously soaking the recipient culture medium group to maintain sufficient moisture; intermittently scratching the surface of the recipient culture medium group, with a scratch depth of 2-5mm, to form a surface structure conducive to spore attachment; the scratches are straight, with a uniform width of 3mm and a depth of 4mm to ensure unobstructed spore inoculation channels; after scratching, using sterile tools to clean debris, and disinfecting the surface with a 0.1%-0.3% methyl permanganate solution, followed by rinsing with clean water; in addition, to further optimize the aeration and nutrient distribution of the culture medium, a 0.2% potassium dihydrogen phosphate solution rich in minerals and trace elements required for the growth of shiitake mushrooms and wood ear mushrooms is evenly sprayed onto the surface of the scratched wood segment; after the scratching treatment, the spore bags are placed in a suitable environment to await spore infection.
[0030] The spore source culture medium group was placed on top of the recipient culture medium group at a distance of 1m. During the spore release period, the temperature was kept stable at 18-24℃ and the humidity was maintained at 92% through artificially constructed environmental control facilities. The air flow monitoring equipment installed in the culture area was used to ensure that the air flow rate was kept in a nearly static state (air flow rate <0.2m / s) for 21 consecutive days.
[0031] In the cultivation of wood ear mushrooms, intelligent environmental control algorithms are used to precisely manage the cultivation environment, for example:
[0032]
[0033]
[0034] When the ambient humidity is detected to be below 80%, the micro-spraying system will be automatically activated, or the system will spray for 3 minutes every 2 hours to increase the ambient humidity and meet the water requirements for the growth of black fungus. When the temperature is above 26℃, the shade net will be opened in time to block some direct sunlight and lower the temperature. At the same time, the ventilation system will be activated to enhance air circulation and remove heat, creating a suitable temperature environment for the growth of black fungus.
[0035] According to professional testing methods, the infection success rate of shiitake mushroom spores on the recipient wood segments reached 99%, indicating that the method of the present invention also has high feasibility and effectiveness in the field of shiitake mushroom cultivation.
[0036]
[0037] Compared with related technologies, the spore solid bacteria inoculation method provided by the present invention has the following beneficial effects:
[0038] This application only requires the preparation of a small amount of bacterial strain, without the need for specialized equipment and a strictly sterile environment, greatly reducing the stringent requirements for hardware facilities and environmental conditions, making the inoculation operation more convenient and easy, thereby reducing production costs. It also eliminates the need for manual operations such as removing the inoculum, stuffing the inoculum, and covering the inoculum, greatly reducing the stringent requirements for hardware facilities and environmental conditions, making the inoculation operation more convenient and easy, reducing the workload of workers while improving work efficiency, and also has the advantage of population.
[0039] By scratching the surface of the recipient culture medium to form specific network fissures, the attachment area of spores is increased, while ensuring the unobstructed spore inoculation channel. Combined with strict control of environmental parameters, such as suitable temperature, humidity, light and ventilation conditions, the success rate of spore infection of the recipient culture medium is significantly improved, thereby increasing economic benefits.
Claims
1. A method for inoculating solid spore-forming bacteria, characterized in that, Includes the following steps: S1. Preparation of culture medium groups: Select trees and divide them into spore source culture medium group and recipient culture medium group; S2. Inducing the formation of mature fruiting bodies in the spore source culture medium group: The target edible fungus strain is inoculated onto the spore source culture medium group to induce the strain to grow and form mature fruiting bodies; S3. Controlling environmental parameters to induce spores to be ejected and fall onto the recipient culture medium; S4. Obtaining fruiting bodies through natural cultivation.
2. The method for inoculating solid spore-forming bacteria according to claim 1, characterized in that, The spore source culture medium accounts for 5%-20% of the total volume of the total culture medium.
3. The method for inoculating solid spore-forming bacteria according to claim 2, characterized in that, When the length of the recipient culture medium group in S1 exceeds 30cm, the surface is subjected to intermittent scratching treatment with a scratch depth of 2-5mm; when the length of the recipient culture medium group does not exceed 30cm, the surface is not subjected to intermittent scratching treatment.
4. The method for inoculating solid spore-forming bacteria according to claim 3, characterized in that, The incision marks are straight lines with a uniform width of 3 mm and a depth of 4 mm to ensure unobstructed spore inoculation channels.
5. The method for inoculating solid spore-forming bacteria according to claim 4, characterized in that, After the scoring is completed, the debris is cleaned up using sterile tools, and the surface is disinfected with a disinfectant.
6. The method for inoculating solid spore-forming bacteria according to claim 5, characterized in that, The disinfectant is a potassium permanganate solution with a concentration of 0.1%-0.3%.
7. The method for inoculating solid spore-forming bacteria according to claim 6, characterized in that, During the spore release period in S2, the air velocity is maintained at <0.2m / s.