Essential oil bacteriostatic hand sanitizer and preparation method thereof

Abstract

The invention provides an essential oil bacteriostatic hand sanitizer and a preparation method thereof. The essential oil bacteriostatic hand sanitizer comprises plant essential oil or plant essential oil and plant extracts. Wherein the plant essential oil is agilawood essential oil and / or rose essential oil, and the plant extract is agilawood extract and / or rose extract. According to the essential oil bacteriostatic hand sanitizer, plant active ingredients are wrapped by microcapsules, and a hand sanitizer system is compounded, so that the prepared essential oil bacteriostatic hand sanitizer has a relatively good bacteriostatic effect on staphylococcus aureus, pseudomonas aeruginosa and candida albicans, and meanwhile, the problem that plant essential oil floats upwards in the hand sanitizer system and is difficult to mix uniformly can be solved; and the suspension stability is good.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of hand sanitizer, in particular, to an essential oil bacteriostatic hand sanitizer and a preparation method thereof. BACKGROUND

[0002] Hand hygiene is the main way to prevent bacteria and viruses from infecting the human body in daily life. In modern life, the demand for hand sanitizer with cleaning and skin care functions is increasing. The bacteriostatic ingredients in commercially available bacteriostatic and disinfectant hand sanitizers are mostly salicylic acid or chlorophenol substances, such as o-phenylphenol (OPP) and trichlorohydroxy diphenyl ether (DP300). Such additives can harm the ecological environment, and long-term use can cause dry and peeling skin.

[0003] Essential oil hand sanitizer, as a personal care product combining cleaning and aromatherapy functions, has seen significant market growth in recent years. Its formula usually includes an aqueous base (such as surfactants, moisturizers), an oil phase component (plant essential oils and fat-soluble active substances), and auxiliary ingredients such as thickening agents and preservatives. However, during production and storage, the suspension stability of essential oil components has become a common technical challenge in the industry. Traditional hand sanitizers achieve cleaning by reducing the surface tension of water through surfactants. However, due to density differences and the immiscibility of oil and water, essential oil components tend to separate, precipitate, or float in the system, leading to a shortened shelf life, uneven distribution of active ingredients, and even local skin irritation when used.

[0004] Hainan agarwood is a rare and precious tree species unique to China, mainly produced in tropical and subtropical regions. The climate of Hainan Island allows the resin to be thick and the fragrance to last. Current artificial agarwood technology is mature, and production is increasing year by year, but there is a lack of deep processing products. Currently, Hainan agarwood essential oil has not been applied in the field of bacteriostatic hand sanitizer. SUMMARY

[0005] To overcome the above-mentioned defects and shortcomings in the prior art, the present application provides an essential oil bacteriostatic hand sanitizer and a preparation method thereof.

[0006] The first object of the present application is to provide a preparation method of an essential oil bacteriostatic hand sanitizer.

[0007] The second object of the present application is to provide an essential oil bacteriostatic hand sanitizer prepared by the above-mentioned preparation method.

[0008] The present application claims the following: A preparation method of an essential oil bacteriostatic hand sanitizer, comprising the following steps: S1. Mix anionic surfactant, glycerol, cocamidopropyl betaine, lauramidopropyl betaine, and vitamin E uniformly to obtain solution A; S2. mixing the plant essential oil and coconut oil, then mixing with the humectant as the core material; mixing the carboxymethyl cellulose and sodium alginate as the wall material, mixing the core material and the wall material to prepare the microcapsule solution B; The plant essential oil is agarwood essential oil and / or rose essential oil. S3. mixing the solution A and the microcapsule solution B to obtain solution C, mixing the solution C with water to obtain the essential oil bacteriostatic hand sanitizer.

[0009] Preferably, the volume fraction of the plant essential oil in the essential oil bacteriostatic hand sanitizer is 0.5-1% by volume.

[0010] Further preferably, the volume fraction of the plant essential oil in the essential oil bacteriostatic hand sanitizer is 0.67% by volume.

[0011] The agarwood essential oil and rose essential oil are volatile, and the active ingredients of the agarwood extract and rose extract are easy to oxidize, resulting in a decrease in the bacteriostatic effect of the product with the storage time. The plant essential oil and plant extract are prepared into microcapsules with a particle size of 5-10 μm by the carboxymethyl cellulose-sodium alginate complex coagulation method, and the wall material thickness is controlled at 1-2 μm. The microcapsules are broken to release the active ingredients when the hands are rubbed, so that the bacteriostatic effect retention rate is better than that of the conventional process. The advantage of this method is that the process is mild, and the materials are naturally degradable, which is suitable for wrapping volatile and oxidizable ingredients. In the essential oil bacteriostatic hand sanitizer of the present application, the loss of active ingredients can be reduced, and the bacteriostatic effect can be prolonged. Through this technology, the problem of essential oil floating and not easy to mix when dissolved in hand sanitizer can be well solved.

[0012] Preferably, in step S2, the plant essential oil is first mixed with coconut oil, then the plant extract is added, and then mixed with the humectant as the core material. The plant extract is agarwood extract and / or rose extract.

[0013] Further preferably, the volume fraction of the plant essential oil in the essential oil bacteriostatic hand sanitizer is 0.2-0.6% by volume, and the volume fraction of the plant extract is 0.1-0.5% by volume.

[0014] More preferably, the volume fraction of the plant essential oil in the essential oil bacteriostatic hand sanitizer is 0.34% by volume, and the volume fraction of the plant extract is 0.34% by volume.

[0015] More preferably, the plant active ingredients are agarwood essential oil, rose essential oil, agarwood extract and rose extract; the volume fraction of the agarwood essential oil is 0.23% by volume, the volume fraction of the rose essential oil is 0.11% by volume, the volume fraction of the agarwood extract is 0.11% by volume, and the volume fraction of the rose extract is 0.23% by volume.

[0016] Preferably, the agarwood essential oil and rose essential oil are extracted by supercritical CO2 extraction method, and the agarwood extract and rose extract are prepared by steam distillation method.

[0017] The agarwood essential oil has unique fragrance and various application values. It contains agarwood alcohol, agarwood furan and other components, has the effects of calming the nerves, relieving pain, antibacterial and anti-inflammatory, regulating emotions, etc., and is widely used in aromatherapy, traditional medicine and daily care. The components act on the limbic system of the brain through olfactory action, can relieve anxiety and help relaxation, the active ingredients can inhibit inflammatory factors and promote healing of small hand wounds; the unique woody fragrance can also balance emotions and improve concentration.

[0018] More preferably, the preparation method of the agarwood essential oil comprises the following steps: placing Hainan agarwood wood into a CO2 supercritical extraction instrument, setting the pressure of the CO2 supercritical extraction instrument to 25-35 Mpa, the temperature to 40-50℃, the outlet valve temperature to 145-155℃, and extracting for 2.5-3.5 h. The preparation method of the rose essential oil comprises the following steps: placing rose flowers into a CO2 supercritical extraction instrument, setting the pressure of the CO2 supercritical extraction instrument to 20-30 Mpa, the temperature to 35-45℃, the outlet valve temperature to 145-155℃, and extracting for 1.5-2.5 h.

[0019] Further preferably, the preparation method of the agarwood essential oil comprises the following steps: placing Hainan agarwood wood into a CO2 supercritical extraction instrument, setting the pressure of the CO2 supercritical extraction instrument to 30 Mpa, the temperature to 45℃, the outlet valve temperature to 150℃, and extracting for 3 h. The preparation method of the rose essential oil comprises the following steps: placing rose flowers into a CO2 supercritical extraction instrument, setting the pressure of the CO2 supercritical extraction instrument to 25 Mpa, the temperature to 41℃, the outlet valve temperature to 150℃, and extracting for 2 h.

[0020] More preferably, the preparation method of the rose extract comprises the following steps: adding rose flowers and distilled water into a steamer according to the solid-liquid ratio of 1 g: (1-3) mL, setting the reflux time to 4-6 min, the distillation temperature to 110-130℃, collecting the distillate after condensation, and removing the upper essential oil to obtain the rose extract. The preparation method of the agarwood extract comprises the following steps: adding Hainan agarwood and distilled water into a steamer according to the solid-liquid ratio of 1 g: (7-9) mL, setting the reflux time to 55-65 min, the distillation temperature to 110-130℃, collecting the distillate after condensation, and removing the upper essential oil to obtain the agarwood extract.

[0021] Further preferably, the preparation method of the rose extract is: adding rose and distilled water into a distillation kettle according to a solid-liquid ratio of 1 g:2 mL, setting a reflux time of 5 min and a distillation temperature of 120 DEG C, collecting the distillate after condensation, and removing the upper essential oil to obtain the rose extract. The preparation method of the eaglewood extract is: adding Hainan eaglewood wood and distilled water into a distillation kettle according to a solid-liquid ratio of 1 g:8 mL, setting a reflux time of 60 min and a distillation temperature of 120 DEG C, collecting the distillate after condensation, and removing the upper essential oil to obtain the eaglewood extract.

[0022] Preferably, in step S1, the anionic surfactant is sodium dodecyl sulfate.

[0023] Preferably, in step S2, the humectant is butylene glycol.

[0024] Sodium dodecyl sulfate: has good foaming performance, can produce abundant foam, helps to improve washing effect, can mix liquid to form stable emulsion, and also has lubricating effect to reduce hand friction; Lauryl amido propyl betaine: is a non-ionic surfactant, which can effectively reduce the charge in the air to reduce static electricity, has low irritation to the skin, and is suitable for various people and can gently remove the cutin and sweat stains on the hands.

[0025] Cocamidopropyl betaine: can reduce the surface tension of water, help remove dirt and grease, has less damage to the skin barrier, is not easy to dry and tight after washing, and is suitable for long-term use. It can be compounded with anion, cation and non-ion to reduce the irritation of other components and improve the stability of the formula. Its raw material is mainly derived from natural coconut oil, has good biodegradability, and has small environmental burden.

[0026] Vitamin E: Vitamin E is a fat-soluble vitamin, which mainly functions as an antioxidant to protect cell membranes from damage by free radicals.

[0027] Glycerol: has strong hygroscopicity, can absorb moisture from the air, and form a protective film on the skin surface to prevent water loss, has the effects of moisturizing skin, improving cell activity, relieving constipation, and antioxidant, and its function is to relieve dry skin.

[0028] Butylene glycol: has similar effects as glycerol, is a common multifunctional ingredient in skin care products, mainly plays the effects of moisturizing, promoting penetration and antibacterial. It helps the skin to lock water through hygroscopicity, and assists the absorption of active ingredients, while gently regulating the skin microenvironment, has high safety and is suitable for various skin types.

[0029] Preferably, in step S1, the anionic surfactant is dissolved in boiling water, and then the glycerol, cocamidopropyl betaine, lauramidopropyl betaine and vitamin E are added when the temperature is reduced to 25-35℃.

[0030] More preferably, in step S1, the anionic surfactant is dissolved in boiling water, and then the glycerol, cocamidopropyl betaine, lauramidopropyl betaine and vitamin E are added when the temperature is reduced to 30℃.

[0031] Preferably, in step S2, the plant essential oil and coconut oil are mixed at 55-65℃, and the volume ratio of the plant essential oil to coconut oil is (0.8-1.2):(0.8-1.2).

[0032] More preferably, in step S2, the plant essential oil and coconut oil are mixed at 60℃, and the volume ratio of the plant essential oil to coconut oil is 1:1.

[0033] Preferably, in the essential oil bacteriostatic hand sanitizer, the concentration of the anionic surfactant is 33-36 g / L, the concentration of glycerol is 20-23 g / L, the concentration of cocamidopropyl betaine is 2.0-2.3 mL / L, the concentration of lauramidopropyl betaine is 2.0-2.3 mL / L, the concentration of vitamin E is 4.3-4.8 g / L, the concentration of humectant is 4.3-4.8 mL / L, the concentration of carboxymethyl cellulose is 0.2-0.3 g / L, and the concentration of sodium alginate is 0.1-0.2 g / L.

[0034] More preferably, in the essential oil bacteriostatic hand sanitizer, the concentration of the anionic surfactant is 35.21 g / L, the concentration of glycerol is 22.01 g / L, the concentration of cocamidopropyl betaine is 2.20 mL / L, the concentration of lauramidopropyl betaine is 2.20 mL / L, the concentration of vitamin E is 4.40 g / L, the concentration of humectant is 4.51 mL / L, the concentration of carboxymethyl cellulose is 0.23 g / L, and the concentration of sodium alginate is 0.11 g / L.

[0035] Preferably, in step S3, the volume ratio of the solution A to the microcapsule solution B is (6.5-8.0):1, and the volume ratio of the solution C to water is (0.8-1.2):(0.8-1.2).

[0036] More preferably, in step S3, the volume ratio of the solution A to the microcapsule solution B is 7.36:1, and the volume ratio of the solution C to water is 1:1.

[0037] The essential oil bacteriostatic hand sanitizer prepared by any of the above preparation methods.

[0038] Compared with the prior art, the present application has the following beneficial effects: The present application provides an essential oil bacteriostatic hand sanitizer and a preparation method thereof.

[0039] In addition, compared with the traditional bacteriostatic hand sanitizer, the essential oil bacteriostatic hand sanitizer prepared by the present application has natural plant extracts as the main active ingredients, does not add any fragrance, has a mild odor, is mild and non-irritating, is friendly to people with rhinitis and sensitive skin, and is safer and healthier for daily use. The essential oil bacteriostatic hand sanitizer of the present application adds carboxymethyl cellulose, sodium alginate, vitamin E, glycerol, etc., to relieve, moisturize and moisturize the skin of the hands while inhibiting bacteria, thereby solving the problem of dry hands after using the hand sanitizer.

[0040] The essential oil bacteriostatic hand sanitizer of the present application uses natural agarwood and / or rose essential oil as the core bacteriostatic ingredient, does not contain synthetic fragrance and irritating surfactants, and has a unique woody aroma derived from the essential oil itself. BRIEF DESCRIPTION OF DRAWINGS

[0041] Figure 1 The bacteriostatic effect of the essential oil bacteriostatic hand sanitizer of Examples 1-14, deionized water of Comparative Example 1 and commercially available bacteriostatic hand sanitizer of Comparative Example 2 on Staphylococcus aureus.

[0042] Figure 2 The bacteriostatic effect of the essential oil bacteriostatic hand sanitizer of Examples 1-14, deionized water of Comparative Example 1 and commercially available bacteriostatic hand sanitizer of Comparative Example 2 on Candida albicans.

[0043] Figure 3 The bacteriostatic effect of the essential oil bacteriostatic hand sanitizer of Examples 1-14, deionized water of Comparative Example 1 and commercially available bacteriostatic hand sanitizer of Comparative Example 2 on Escherichia coli.

[0044] Figure 4 The bacteriostatic effect of the essential oil bacteriostatic hand sanitizer of Examples 1-14, deionized water of Comparative Example 1 and commercially available bacteriostatic hand sanitizer of Comparative Example 2 on Pseudomonas aeruginosa.

[0045] Figure 5 The essential oil suspension stability results of the essential oil bacteriostatic hand sanitizer of Examples 11, Comparative Example 3 and Comparative Example 4 after standing for 2 days. DETAILED DESCRIPTION

[0046] The application will be further described in conjunction with specific examples, but the examples do not limit the application in any form. Unless otherwise specified, the reagents, methods and equipment used in the application are conventional reagents, methods and equipment in the art.

[0047] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0048] Lignum Aquilariae Resinatum Essential Oil: Dry Hainan Aquilaria wood material was crushed with a crusher, and then loaded into an extraction kettle installed in a CO2 supercritical extraction instrument. The CO2 supercritical extraction instrument was set at a pressure of 30 Mpa, a temperature of 45℃, and an outlet valve temperature of 150℃. Continuous extraction was performed for 3 hours. Rose Essential Oil: Dry rose flowers were crushed with a crusher, and then loaded into an extraction kettle installed in a CO2 supercritical extraction instrument. The CO2 supercritical extraction instrument was set at a pressure of 25 Mpa, a temperature of 41℃, and an outlet valve temperature of 150℃. Continuous extraction was performed for 2 hours. Rose Extract: Fresh gold-edged rose samples were placed in a steam pot, and distilled water was added at a solid-liquid ratio of 1 g:2 mL. The reflux time was set to 5 minutes, and the distillation temperature was set to 120℃. The distillate was collected after condensation, and the upper essential oil was removed using a pipette gun to obtain the gold-edged rose extract. Aquilaria Extract: Hainan Aquilaria wood material was placed in a steam pot, and distilled water was added at a solid-liquid ratio of 1 g:8 mL. The reflux time was set to 60 minutes, and the distillation temperature was set to 120℃. The distillate was collected after condensation, and the upper essential oil was removed using a pipette gun to obtain the Aquilaria extract (Aquilaria volatile component content is low and slow release, increasing water volume can improve extraction efficiency, avoid low component concentration, and Aquilaria components are wrapped in wood fibers, which need to be extended to ensure sufficient extraction and avoid effective component residues).

[0049] Example 1: Preparation method of essential oil bacteriostatic hand sanitizer The present example provides a preparation method of essential oil bacteriostatic hand sanitizer, which specifically comprises the following steps: S1. 100 mL of deionized water was heated to boiling, and 8 g of sodium dodecyl sulfate was added to mix evenly. When the temperature of the mixed solution decreased to 30℃, 5 g of glycerol, 500 μL of cocamide propyl betaine, 500 μL of lauramide propyl betaine and 1 g of vitamin E were added, and stirred evenly to obtain solution A; S2. Take 30 μL of the Agarwood essential oil, add coconut oil in a volume ratio of 1:1 under the condition of a 60°C water bath, then mix with 20 μL of butylene glycol to obtain a mixed solution. Add the mixed solution to 100 μL of a 1% carboxymethyl cellulose solution (w / v) and then mix with 100 μL of a 0.5% sodium alginate solution (w / v) to obtain solution B; S3. Mix 1950 μL of solution A with 280 μL of solution B to obtain solution C, and then mix solution C with deionized water in a volume ratio of 1:1 to obtain the essential oil antibacterial hand sanitizer.

[0050] Example 2: A preparation method of an essential oil antibacterial hand sanitizer The present example provides a preparation method of an essential oil antibacterial hand sanitizer, which specifically comprises the following steps: S1. Perform according to Example 1; S2. Mix 20 μL of the Agarwood essential oil and 10 μL of the Rose essential oil, add coconut oil in a volume ratio of 1:1 under the condition of a 60°C water bath, then mix with 20 μL of butylene glycol to obtain a mixed solution. Add the mixed solution to 100 μL of a 1% carboxymethyl cellulose solution (w / v) and then mix with 100 μL of a 0.5% sodium alginate solution (w / v) to obtain solution B; S3. Mix 1950 μL of solution A with 280 μL of solution B to obtain solution C, and then mix solution C with deionized water in a volume ratio of 1:1 to obtain the essential oil antibacterial hand sanitizer.

[0051] Example 3: A preparation method of an essential oil antibacterial hand sanitizer The present example provides a preparation method of an essential oil antibacterial hand sanitizer, which specifically comprises the following steps: S1. Perform according to Example 1; S2. Take 30 μL of the Agarwood essential oil, add coconut oil in a volume ratio of 1:1 under the condition of a 60°C water bath, then mix with 20 μL of butylene glycol and 10 μL of the Agarwood extract to obtain a mixed solution. Add the mixed solution to 100 μL of a 1% carboxymethyl cellulose solution (w / v) and then mix with 100 μL of a 0.5% sodium alginate solution (w / v) to obtain solution B; S3. Mix 1950 μL of solution A with 270 μL of solution B to obtain solution C, and then mix solution C with deionized water in a volume ratio of 1:1 to obtain the essential oil antibacterial hand sanitizer.

[0052] Example 4: A preparation method of an essential oil antibacterial hand sanitizer The present example provides a preparation method of an essential oil antibacterial hand sanitizer, which specifically comprises the following steps: S1. Perform according to Example 1; S2. Take 20 μL of the linaloe oil, add coconut oil in a volume ratio of 1:1 under the condition of a 60°C water bath, then mix with 20 μL of butylene glycol and 10 μL of the rose extract to obtain a mixed solution. Add the mixed solution to 100 μL of a 1% carboxymethyl cellulose solution (w / v) and then mix with 100 μL of a 0.5% sodium alginate solution (w / v) to obtain solution B; S3. Mix 1950 μL of solution A with 270 μL of solution B to obtain solution C, and then mix solution C with deionized water in a volume ratio of 1:1 to obtain the essential oil bacteriostatic hand sanitizer.

[0053] Example 5: A method for preparing an essential oil bacteriostatic hand sanitizer The present example provides a method for preparing an essential oil bacteriostatic hand sanitizer, which specifically comprises the following steps: S1. Perform according to Example 1; S2. Take 20 μL of the linaloe oil and 5 μL of the rose oil, mix them, add coconut oil in a volume ratio of 1:1 under the condition of a 60°C water bath, then mix with 20 μL of butylene glycol and 5 μL of the linaloe extract to obtain a mixed solution. Add the mixed solution to 100 μL of a 1% carboxymethyl cellulose solution (w / v) and then mix with 100 μL of a 0.5% sodium alginate solution (w / v) to obtain solution B; S3. Mix 1950 μL of solution A with 275 μL of solution B to obtain solution C, and then mix solution C with deionized water in a volume ratio of 1:1 to obtain the essential oil bacteriostatic hand sanitizer.

[0054] Example 6: A method for preparing an essential oil bacteriostatic hand sanitizer The present example provides a method for preparing an essential oil bacteriostatic hand sanitizer, which specifically comprises the following steps: S1. Perform according to Example 1; S2. Take 20 μL of the linaloe oil, add coconut oil in a volume ratio of 1:1 under the condition of a 60°C water bath, then mix with 20 μL of butylene glycol, 5 μL of the linaloe extract, and 5 μL of the rose extract to obtain a mixed solution. Add the mixed solution to 100 μL of a 1% carboxymethyl cellulose solution (w / v) and then mix with 100 μL of a 0.5% sodium alginate solution (w / v) to obtain solution B; S3. Mix 1950 μL of solution A with 270 μL of solution B to obtain solution C, and then mix solution C with deionized water in a volume ratio of 1:1 to obtain the essential oil bacteriostatic hand sanitizer.

[0055] Example 7: A method for preparing an essential oil bacteriostatic hand sanitizer The present example provides a method for preparing an essential oil bacteriostatic hand sanitizer, which specifically comprises the following steps: S1. Proceed according to Example 1; S2. Take 20 μL of the linaloe oil and 5 μL of the rose oil, add coconut oil in a volume ratio of 1:1 under the condition of a 60°C water bath, then mix with 20 μL of butylene glycol and 5 μL of rose extract to obtain a mixed solution. Add the mixed solution to 100 μL of a 1% carboxymethyl cellulose solution (w / v) and then mix into 100 μL of a 0.5% sodium alginate solution (w / v) to obtain solution B; S3. Mix 1950 μL of solution A with 275 μL of solution B uniformly to obtain solution C, and mix solution C with deionized water in a volume ratio of 1:1 to obtain the essential oil bacteriostatic hand sanitizer.

[0056] Example 8 A preparation method of an essential oil bacteriostatic hand sanitizer The present example provides a preparation method of an essential oil bacteriostatic hand sanitizer, which specifically comprises the following steps: S1. Proceed according to Example 1; S2. Take 20 μL of the linaloe oil and 5 μL of the rose oil, add coconut oil in a volume ratio of 1:1 under the condition of a 60°C water bath, then mix with 20 μL of butylene glycol and 5 μL of rose extract to obtain a mixed solution. Add the mixed solution to 100 μL of a 1% carboxymethyl cellulose solution (w / v) and then mix into 100 μL of a 0.5% sodium alginate solution (w / v) to obtain solution B; S3. Mix 1950 μL of solution A with 275 μL of solution B uniformly to obtain solution C, and mix solution C with deionized water in a volume ratio of 1:1 to obtain the essential oil bacteriostatic hand sanitizer.

[0057] Example 9 A preparation method of an essential oil bacteriostatic hand sanitizer The present example provides a preparation method of an essential oil bacteriostatic hand sanitizer, which specifically comprises the following steps: S1. Proceed according to Example 1; S2. Take 20 μL of the linaloe oil and 5 μL of the rose oil, add coconut oil in a volume ratio of 1:1 under the condition of a 60°C water bath, then mix with 20 μL of butylene glycol and 5 μL of rose extract to obtain a mixed solution. Add the mixed solution to 100 μL of a 1% carboxymethyl cellulose solution (w / v) and then mix into 100 μL of a 0.5% sodium alginate solution (w / v) to obtain solution B; S3. Mix 1950 μL of solution A with 275 μL of solution B uniformly to obtain solution C, and mix solution C with deionized water in a volume ratio of 1:1 to obtain the essential oil bacteriostatic hand sanitizer.

[0058] Example 10 A preparation method of an essential oil bacteriostatic hand sanitizer The embodiment provides a preparation method of an essential oil bacteriostatic hand sanitizer, and specifically comprises the following steps: S1. According to embodiment 1; S2. 10 μL of the agarwood essential oil is taken, coconut oil is added in a volume ratio of 1:1 under the condition of a 60℃ water bath, and then 20 μL of butylene glycol, 10 μL of agarwood extract and 10 μL of rose extract are mixed to obtain a mixed solution. The mixed solution is added into 100 μL of 1% carboxymethyl cellulose solution (w / v) and then into 100 μL of 0.5% sodium alginate solution (w / v) to obtain solution B; S3. 1950 μL of solution A is uniformly mixed with 260 μL of solution B to obtain solution C, and then solution C is uniformly mixed with deionized water in a volume ratio of 1:1 to obtain the essential oil bacteriostatic hand sanitizer.

[0059] Embodiment 11 The embodiment provides a preparation method of an essential oil bacteriostatic hand sanitizer, and specifically comprises the following steps: S1. According to embodiment 1; S2. 10 μL of the agarwood essential oil and 5 μL of rose essential oil are taken, coconut oil is added in a volume ratio of 1:1 under the condition of a 60℃ water bath, and then 20 μL of butylene glycol, 5 μL of agarwood extract and 10 μL of rose extract are mixed to obtain a mixed solution. The mixed solution is added into 100 μL of 1% carboxymethyl cellulose solution (w / v) and then into 100 μL of 0.5% sodium alginate solution (w / v) to obtain solution B; S3. 1950 μL of solution A is uniformly mixed with 260 μL of solution B to obtain solution C, and then solution C is uniformly mixed with deionized water in a volume ratio of 1:1 to obtain the essential oil bacteriostatic hand sanitizer.

[0060] Embodiment 12 The embodiment provides a preparation method of an essential oil bacteriostatic hand sanitizer, and specifically comprises the following steps: S1. According to embodiment 1; S2. 10 μL of the agarwood essential oil and 5 μL of rose essential oil are taken, coconut oil is added in a volume ratio of 1:1 under the condition of a 60℃ water bath, and then 20 μL of butylene glycol, 5 μL of agarwood extract and 10 μL of rose extract are mixed to obtain a mixed solution. The mixed solution is added into 100 μL of 1% carboxymethyl cellulose solution (w / v) and then into 100 μL of 0.5% sodium alginate solution (w / v) to obtain solution B; S3. 1950 μL solution A is mixed with 270 μL solution B to obtain solution C, and solution C is mixed with deionized water according to the volume ratio of 1:1 to obtain the essential oil bacteriostatic hand sanitizer.

[0061] Example 13 A method for preparing an essential oil bacteriostatic hand sanitizer The present example provides a method for preparing an essential oil bacteriostatic hand sanitizer, which specifically comprises the following steps: S1. According to Example 1; S2. 10 μL of agarwood essential oil and 10 μL of rose essential oil are added to coconut oil according to the volume ratio of 1:1 under the condition of a 60°C water bath, and then mixed with 20 μL of butylene glycol, 5 μL of agarwood extract, and 5 μL of rose extract to obtain a mixed solution. The mixed solution is added to 100 μL of 1% carboxymethyl cellulose solution (w / v) and then mixed with 100 μL of 0.5% sodium alginate solution (w / v) to obtain solution B; S3. 1950 μL solution A is mixed with 270 μL solution B to obtain solution C, and solution C is mixed with deionized water according to the volume ratio of 1:1 to obtain the essential oil bacteriostatic hand sanitizer.

[0062] Example 14 A method for preparing an essential oil bacteriostatic hand sanitizer The present example provides a method for preparing an essential oil bacteriostatic hand sanitizer, which specifically comprises the following steps: S1. According to Example 1; S2. 10 μL of rose essential oil is added to coconut oil according to the volume ratio of 1:1 under the condition of a 60°C water bath, and then mixed with 20 μL of butylene glycol, 10 μL of agarwood extract, and 10 μL of rose extract to obtain a mixed solution. The mixed solution is added to 100 μL of 1% carboxymethyl cellulose solution (w / v) and then mixed with 100 μL of 0.5% sodium alginate solution (w / v) to obtain solution B; S3. 1950 μL solution A is mixed with 270 μL solution B to obtain solution C, and solution C is mixed with deionized water according to the volume ratio of 1:1 to obtain the essential oil bacteriostatic hand sanitizer.

[0063] Comparative Example 1 2 mL of deionized water.

[0064] Comparative Example 2 2 mL of a commercially available bacteriostatic hand sanitizer.

[0065] Comparative Example 3 The present comparative example provides a method for preparing a hand sanitizer according to Example 11, which specifically comprises the following steps: S1. According to Example 1; S2. Take 10 μL of agarwood essential oil and 5 μL of rose essential oil, add coconut oil at a volume ratio of 1:1 under the condition of a 60°C water bath, then mix with 20 μL of butylene glycol, 5 μL of agarwood extract, and 10 μL of rose extract to obtain a mixed solution. Add the mixed solution to 100 μL of 1% carboxymethyl cellulose solution (w / v) to obtain solution B; S3. Mix 1950 μL of solution A with 165 μL of solution B uniformly to obtain solution C, and mix solution C with deionized water at a volume ratio of 1:1 to obtain an essential oil antibacterial hand sanitizer.

[0066] Comparative Example 4 This comparative example provides a preparation method of a hand sanitizer according to Example 11, specifically comprising the following steps: S1. Heat 100 mL of deionized water to boiling, and add 8 g of sodium dodecyl sulfate to mix uniformly. When the temperature of the mixed solution decreases to 30°C, add 5 g of glycerol, 1 g of betaine, 0.1 g of allantoin, and 1 g of vitamin E, and stir uniformly to obtain solution A; S2. Take 10 μL of agarwood essential oil and 5 μL of rose essential oil, add coconut oil at a volume ratio of 1:1 under the condition of a 60°C water bath, then mix with 20 μL of butylene glycol, 5 μL of agarwood extract, and 10 μL of rose extract to obtain a mixed solution. Add the mixed solution to 100 μL of 1% hyaluronic acid solution (w / v) and then mix into 100 μL of 0.5% sodium alginate solution (w / v) to obtain solution B; S3. Mix 1950 μL of solution A with 265 μL of solution B uniformly to obtain solution C, and mix solution C with deionized water at a volume ratio of 1:1 to obtain an essential oil antibacterial hand sanitizer.

[0067] Test Example 1: Antibacterial experiment I. Experimental method 1. Prepare sterile filter paper sheets, and use a puncher to punch the filter paper into 6 mm diameter circular paper sheets. After high-pressure sterilization, immerse the filter paper sheets in the essential oil antibacterial hand sanitizers of Examples 1-14, deionized water of Comparative Example 1, and commercially available antibacterial hand sanitizer of Comparative Example 2 for 12 h for use; 2. Prepare LB medium: weigh 5 g of tryptone, 2.5 g of yeast extract, 5 g of NaCl, and 7.5 g of agar into a 900 mL beaker, add water and heat to completely melt, and continuously stir, use pH test paper to test the acidity and alkalinity, and use 1% NaOH solution (w / v) to adjust the pH to 7. Make up to 500 mL with deionized water, and high-pressure sterilize at 101 kPa and 121°C for 20 min. Complete the pour plate operation on a clean bench to obtain LB medium for Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa antibacterial experiments; Preparation of Sabouraud medium: weigh 15 g of Sabouraud broth medium, 10 g of glucose and 7.5 g of agar powder, add water to 500 mL, heat and stir until completely melted, adjust the pH to 5 with 1% NaOH solution (w / v). High pressure sterilization at 101 kPa, 121℃ for 20 min, complete the pouring plate operation on the clean bench, obtain the Sabouraud medium for Candida albicans inhibition test; 3. Take the frozen S. aureus, E. coli, P. aeruginosa and C. albicans tubes out of the -80℃ refrigerator, and melt at room temperature. On the clean bench, add 1 mL of S. aureus, E. coli and P. aeruginosa bacterial solution to 25 mL of LB liquid medium, and add C. albicans to 25 mL of Sabouraud liquid medium, and place in a constant temperature shaker (180 r / min) for 24 h of shaking culture, and store the bacterial solution in the refrigerator for standby; 4. Preparation of bacterial suspension and plate counting: sterilize 10 mL centrifuge tubes and 0.9% normal saline (w / v). Use the method of dilution by ratio: take 8 10 mL centrifuge tubes, label 10 1 ~ 10 8 , and place in the test tube rack. Take 1 mL of bacterial solution and add to the first centrifuge tube, then add 9 mL of 0.9% normal saline (w / v), mix well. Take 1 mL of the mixed solution from the first centrifuge tube and add to 9 mL of 0.9% normal saline (w / v) in the second centrifuge tube, and so on, to complete the dilution by ratio of 10 1 ~ 10 8 .

[0068] Take 100 μL of bacterial solution of each concentration and spread on solid medium, 2 repeats are needed for each concentration. Gently roll the glass beads to evenly attach the bacterial solution to the surface of the medium, repeat the spreading until the area of the bacterial solution is uniform and completely covered. Then place the S. aureus, E. coli and P. aeruginosa bacterial solution in a 37℃ incubator for 20 h, and the C. albicans bacterial solution in a 28℃ incubator for 20 h, and count to select bacterial suspensions with S. aureus colony count of 10 6 CFU / mL, E. coli colony count of 10 6 CFU / mL, P. aeruginosa colony count of 10 5 CFU / mL, and C. albicans colony count of 10 6 CFU / mL, for standby; 5. Perform the bacteriostatic experiment, under sterile conditions, use a pipette to suck 100 μL of the prepared bacterial suspension, use glass beads to evenly spread, place 3 pieces of the soaked filter paper prepared in step 1 on the culture medium, each with two repeats; place the culture dish into a constant temperature incubator and incubate for 24 h, place Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa into a 37℃ incubator, and place Candida albicans into a 28℃ incubator, take out and measure the diameter of the inhibition zone with a ruler, take the average value to obtain the bacteriostatic effect of each group of hand sanitizer.

[0069] II. Experimental results The results are shown in Table 1 and Figures 1-4 The results show that the essential oil bacteriostatic hand sanitizer of Examples 1-14 has a certain bacteriostatic effect on Candida albicans and Staphylococcus aureus, and the essential oil bacteriostatic hand sanitizer of Examples 9-11 has the best effect.

[0070] Table 1 Bacteriostatic effect

[0071] Test Example 2 Human hand bacteriostatic experiment I. Experimental method The subjects use the essential oil bacteriostatic hand sanitizer of Example 11, the deionized water of Comparative Example 1 and the commercially available bacteriostatic hand sanitizer of Comparative Example 2 to clean each finger of the left and right hands, and then use a high-pressure sterilized non-woven fabric to wipe the hands after washing with running water, and then use a non-woven fabric to randomly wipe the fingers of the left and right hands of the subjects within 0 h, 1 h, 2 h, 3 h and 4 h, and then place the non-woven fabric into a conical flask containing LB broth medium, and incubate in a constant temperature incubator for 18 h, and then record the OD 600 value by ultraviolet spectrophotometry, so as to compare the bacteriostatic efficacy.

[0072] II. Experimental results OD 600 The results are shown in Table 2, which shows that the essential oil bacteriostatic hand sanitizer of Example 11 has a lower bacterial concentration growth value within 0-4 h than Comparative Examples 1 and 2, indicating that the essential oil bacteriostatic hand sanitizer of the present application can better and more effectively inhibit the growth of bacteria.

[0073] Table 2 Bacteriostatic efficacy

[0074] Test Example 3 Foaming power test I. Experimental method The foaming power of the essential oil bacteriostatic hand sanitizer prepared in Examples 1-14 is measured by the cylinder method, i.e. the Bartsh method, and the specific method is as follows: Take 5 mL of the essential oil bacteriostatic hand sanitizer, add 45 mL of deionized water, pour into a 250 mL measuring cylinder, cover, shake 10 times within 5 s, read the initial height of the foam, and thus determine the foaming power of the essential oil bacteriostatic hand sanitizer.

[0075] II. Experimental results The foaming power test results show that the essential oil bacteriostatic hand sanitizers prepared in Examples 1-14 all have a foaming power of more than 150 mL, indicating that the essential oil bacteriostatic hand sanitizers prepared all have good foaming performance.

[0076] Test Example 4: Essential oil suspension stability test I. Experimental method Take the same volume of essential oil bacteriostatic hand sanitizers of Example 11, Comparative Example 3 and Comparative Example 4, centrifuge at 5000 r / min for 10 min, and observe the essential oil suspension stability of the essential oil bacteriostatic hand sanitizer.

[0077] II. Experimental results As shown in Table 3 and Figure 5 The results show that the essential oil bacteriostatic hand sanitizers of Comparative Examples 3 and 4 always cannot solve the problem of essential oil suspension, even at the time of preparation (0-day standing), the system is prone to local essential oil aggregation; during 1-day to 2-day standing, the essential oil floating phenomenon becomes more and more obvious, the surface gradually forms an oil film visible to the naked eye, and is difficult to mix with the hand sanitizer base, completely unable to meet the product uniformity requirements.

[0078] While the essential oil bacteriostatic hand sanitizer prepared in Example 11 has achieved a high degree of uniformity at 0-day standing (immediately), without essential oil aggregation or floating signs; even after 1-day and 2-day standing, it can still maintain a stable suspension state without any oil phase separation, which shows that the essential oil bacteriostatic hand sanitizer of the present application solves the inherent problems of essential oil floating and difficult mixing in the traditional compounding method.

[0079] Table 3: Essential oil suspension stability test

[0080] Test Example 5: Safety evaluation and use feeling evaluation I. Experimental method Select 20 subjects (10 males and 10 females aged 18-45 years old without skin disease and cosmetic allergy history) to evaluate the safety of the essential oil bacteriostatic hand sanitizer prepared in Example 11. The subjects use an appropriate amount of hand sanitizer to rub both hands for 1 min, then rinse with clean water for 1 min, then evaluate the safety of the essential oil bacteriostatic hand sanitizer according to the skin reaction of the subjects (Table 4), and evaluate the use feeling of the essential oil bacteriostatic hand sanitizer according to the use feeling of the subjects (Table 5).

[0081] Table 4 Safety evaluation criteria

[0082] Table 5 Evaluation results of use feeling

[0083] II. Experimental results The safety evaluation results are shown in Table 6. No erythema and edema reactions occurred after the subjects washed their hands with the essential oil bacteriostatic hand sanitizer. This indicates that the hand sanitizer is mild and non-irritating.

[0084] Table 6 Safety evaluation results

[0085] The evaluation results of use feeling are shown in Table 7. The results show that 85% of the users experienced excellent use feeling. Some users fed back that the agarwood essential oil has a unique woody fragrance and the fragrance can relieve anxiety and calm the nerves.

[0086] Table 7 Evaluation results of use feeling

[0087] The above examples are preferred embodiments of the present application, but the embodiments of the present application are not limited to the above examples. Any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present application are equivalent replacement methods and are included in the protection scope of the present application.

Claims

1. A method for preparing an essential oil antibacterial hand sanitizer, characterized in that, Includes the following steps: S1. Mix anionic surfactant, glycerin, cocamidopropyl betaine, laurylamidopropyl betaine and vitamin E evenly to obtain solution A; S2. Mix plant essential oils and coconut oil, then mix with a moisturizer to form the core material; use carboxymethyl cellulose and sodium alginate as the wall material, and mix the core material and wall material to form microcapsule solution B; The plant essential oil is agarwood essential oil and / or rose essential oil; S3. Mix solution A with microcapsule solution B to obtain solution C. Mix solution C with water to obtain essential oil antibacterial hand sanitizer.

2. The preparation method according to claim 1, characterized in that, In step S2, after the plant essential oil is mixed with coconut oil, plant extracts can be added, and then mixed with a moisturizer as a core material. The plant extract is agarwood extract and / or rose extract.

3. The preparation method according to claim 2, characterized in that, The agarwood essential oil and rose essential oil were obtained by supercritical CO2 extraction, and the agarwood extract and rose extract were prepared by steam distillation.

4. The preparation method according to claim 1, characterized in that, In step S1, the anionic surfactant is sodium dodecyl sulfate.

5. The preparation method according to claim 1, characterized in that, In step S2, the humectant is butylene glycol.

6. The preparation method according to claim 1, characterized in that, The essential oil antibacterial hand sanitizer contains anionic surfactants at a concentration of 33–36 g / L, glycerin at a concentration of 20–23 g / L, cocamidopropyl betaine at a concentration of 2.0–2.3 mL / L, lauramide propyl betaine at a concentration of 2.0–2.3 mL / L, vitamin E at a concentration of 4.3–4.8 g / L, a moisturizer at a concentration of 4.3–4.8 mL / L, carboxymethyl cellulose at a concentration of 0.2–0.3 g / L, and sodium alginate at a concentration of 0.1–0.2 g / L.

7. The preparation method according to claim 1, characterized in that, In the aforementioned essential oil antibacterial hand sanitizer, the volume fraction of plant essential oil is 0.5% to 1%.

8. The preparation method according to claim 2, characterized in that, In the aforementioned essential oil antibacterial hand sanitizer, the volume fraction of plant essential oil is 0.2-0.6%, and the volume fraction of plant extract is 0.1-0.5%.

9. The preparation method according to claim 1, characterized in that, In step S3, the volume ratio of solution A to microcapsule solution B is (6.5-8.0):1, and the volume ratio of solution C to water is (0.8-1.2):(0.8-1.2).

10. The essential oil antibacterial hand sanitizer prepared by any one of the preparation methods according to claims 1 to 9.

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