Skin repair composition and preparation method thereof

Abstract

The invention relates to a skin repair composition and a preparation method thereof, and relates to the field of skin care products. The skin repair composition can quickly repair the sub-health state of the skin through the synergistic effect of multiple active components, has higher bioavailability and skin permeability, can accurately act on the deep layer of the skin to perform DNA-level repair, ensures the safety, stability and effectiveness of the product through the optimized preparation process, and is suitable for industrial production. Wide application prospects are realized.

Description

Technical Field

[0001] This invention belongs to the field of skin care products, and more specifically relates to a skin repair composition and its preparation method. Background Technology

[0002] In the field of skin repair, current products on the market mainly focus on one aspect of efficacy, without forming a comprehensive repair system. They ignore the fact that skin repair is a complex, multi-stage process, including cell nutrition supply, inflammation control, and skin structure remodeling, which leads to poor skin repair effects.

[0003] In view of the shortcomings of the prior art, the present invention proposes a skin repair composition, its preparation method and application. Summary of the Invention

[0004] In view of the above-mentioned defects and problems of the prior art, the present invention has developed a skin repair composition and its preparation method.

[0005] The first aspect of the present invention provides a skin repair composition, comprising, by weight, the following components: 0.1-12 parts sodium hyaluronate, 0.05-0.36 parts cell nutrient solution, 0.001-20 parts superassembled PDRN, 0.01-10 parts milk vesicles, active ingredients, and water; wherein the cell nutrient solution comprises amino acids, vitamins, buffers, and β-glucan.

[0006] Optionally, the amino acids include lysine, histidine, arginine, aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, isoleucine, leucine, tyrosine, and phenylalanine.

[0007] Optionally, it may also include 0.06-10 parts by weight of ginseng root extract.

[0008] Optionally, by weight, the sodium hyaluronate is 0.1-0.5 parts, the cell nutrient solution is 0.05-0.11 parts, the superassembled PDRN is 0.001-15 parts, and the milk vesicles are 0.01-5 parts.

[0009] Optionally, one or more of the following conditions must be met: (a) The active ingredients include skin feelers and / or moisturizers; (b) The effective ingredient is 0.4-18 parts by weight; (c) The buffer comprises citric acid and trisodium citrate; (d) The vitamins mentioned include vitamin B12 and nicotinamide.

[0010] Optionally, one or more of the following conditions must be met: (e) The humectant includes one or more of betaine, glycerin, 1,2-pentanediol, allantoin and dipotassium glycyrrhizate; (f) The skin-feeling agent includes one or more of glyceryl glucoside, glycol glucoside, hydrogenated lecithin, and cholesterol; (g) By weight, the moisturizer is 0.5-13 parts and the skin feeler is 0.01-5 parts.

[0011] In another aspect, the present invention provides a method for preparing the above-mentioned composition, comprising the following steps: S1. Mix sodium hyaluronate and water thoroughly to obtain phase A; S2. Add the active ingredient to phase A, mix well, and obtain phase B; S3. Add superassembled PDRN, milk vesicles and cell nutrient solution to phase B, stir, and obtain the composition.

[0012] Preferably, one or more of the following conditions are met: (h) In step S1, stir at 80-85℃ at a speed of 600-1000 rpm; (i) In step S3, after phase B cools down to 40-45°C, add superassembled PDRN, milk vesicles and cell nutrient solution; (j) In step S3, the stirring speed is 600-1000 rpm and the stirring time is 20-40 min; (k) In step S3, ginseng root extract is also added to phase B.

[0013] In another aspect, the present invention also provides a product comprising the above-described composition, the product comprising a personal care product, more preferably, the product further comprising personal care product-acceptable excipients; more preferably, the personal care product comprises a skin care product.

[0014] Preferably, the skin care product includes lotion, facial mask liquid, face cream and / or serum.

[0015] In summary, this application has the following beneficial effects: (1) Through the synergistic effect of cell nutrient solution, super-assembled PDRN and milk vesicles, multiple active ingredients can comprehensively and quickly repair the sub-healthy state of the skin, with higher bioavailability and skin permeability, and can accurately act on the deep layer of the skin to repair DNA, promoting rapid and efficient repair of the skin. (2) The composition has significant effects on improving skin diseases in people with sensitive skin, such as gentleness, repair and soothing, and has broad application prospects; (3) The preparation method of the composition has been optimized, making it easier to operate while ensuring product safety, stability and effectiveness, and suitable for industrial promotion. Detailed Implementation

[0016] The present application will be further described in detail below with reference to the embodiments. It should be noted that: unless otherwise specified, the conditions in the following embodiments are performed under conventional conditions or conditions recommended by the manufacturer, and the raw materials used in the following embodiments are all from commercially available sources unless otherwise specified.

[0017] To make the above-mentioned objectives, features, and advantages of this application more apparent and understandable, a detailed description of specific embodiments of this application is provided below. Many specific details are set forth in the following description to provide a thorough understanding of this application. However, this application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this application. Therefore, this application is not limited to the specific embodiments disclosed below.

[0018] In this application, "optionally," "optionally," and "optional" mean that something is optional, that is, it means that it is selected from either "with" or "without." If there are multiple "optional" entries in a technical solution, unless otherwise specified, and there are no contradictions or mutual constraints, each "optional" entry shall be independent.

[0019] In this application, numerical ranges are referred to as continuous unless otherwise specified, and include the minimum and maximum values ​​of the range, as well as every value between the minimum and maximum values. Furthermore, when the range refers to integers, it includes every integer between the minimum and maximum values ​​of the range. Additionally, when multiple ranges are provided to describe a feature or characteristic, the ranges may be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges to which they are incorporated.

[0020] In this application, the technical features described in an open-ended manner include both closed technical solutions consisting of the listed features and open technical solutions that include the listed features.

[0021] Unless otherwise defined, all technical and scientific terms used in this application have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used in this application and in its specification is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. The term "and / or" as used in this application includes any and all combinations of one or more of the associated listed items. The term "multiple" in this application means at least two, such as two, three, etc., unless otherwise expressly specified.

[0022] To facilitate the explanation of the technical solution of this application, the following materials will be described before starting the experiments of this application: (1) Sodium hyaluronate: a natural polysaccharide widely found in human connective tissue and extracellular matrix, possessing various important physiological functions. Sodium hyaluronate has extremely strong water absorption properties, forming a moisturizing film on the skin surface to prevent moisture loss, while simultaneously attracting external moisture to keep the skin hydrated. The sodium hyaluronate used in this invention was purchased from Shandong Anhua Biotechnology Co., Ltd., with a molecular weight of 200,000 Da to 500,000 Da.

[0023] (2) The various amino acids in the cell nutrient solution are the basic building blocks of proteins, participating in the construction of cell structure and function. The vitamins (vitamin B12 and nicotinamide) contained therein can act as coenzymes and cofactors to participate in cell metabolism, playing an important role in cell growth and repair. β-glucan can provide energy for cells. The buffer system containing citric acid and trisodium citrate can provide the cell nutrient solution with a pH range consistent with human body fluids, thereby optimizing the cell growth environment and promoting cell regeneration. By providing necessary nutrients, it supports cell self-replication and differentiation, helps tissue repair and regeneration, enhances cell vitality, provides the energy and nutrients required by cells, improves cell metabolic efficiency, and enhances cell activity and survival ability. In addition, when combined with super-assembled PDRN, milk vesicles and sodium hyaluronate, it can also regulate the body's immune response, reduce inflammatory response, and enhance the body's defense against pathogens. The resulting skin repair composition can comprehensively and efficiently repair the skin, making the skin supple and radiant.

[0024] (3) The milk vesicles used in this invention are polymicro® milk phospholipid vesicle extracts, purchased from Shaanxi Weimi Biotechnology Co., Ltd. Polymicro® milk phospholipid vesicle extracts are extracted from fresh milk using multiple purification and separation techniques to obtain extracellular vesicles secreted by bovine mammary epithelial cells. These vesicles contain active substances such as RNA, proteins, and lipids, and have a phospholipid bilayer structure with an average particle size between 150 nm and 170 nm. A low-temperature process is used to maximize the preservation of natural active ingredients. Polymicro® milk phospholipid vesicle extract has a significant whitening effect, inhibiting melanin production at its source through multiple pathways. Its encapsulated miR-2478 can specifically bind to Rap1a, inhibiting the expression of the key gene MITF in melanin production via the GSK-3β pathway, thus blocking melanin synthesis at its source. In addition, it has a stabilizing effect on cell membranes, enhancing the antioxidant capacity of cells, resisting free radical damage to the skin, and delaying skin aging.

[0025] (4) The superassembled PDRN used was Polyglycin® superassembled PDRN, purchased from Shaanxi Baihong Synthetic Biotechnology Co., Ltd., with a molecular weight of 297 kDa. Polyglycin® superassembled PDRN utilizes Polyglycin® microsphere technology, which employs a stacked microsphere controlled-release technology platform. Using polysaccharides or derived targeted polysaccharides as precursors, and through the artificial design of weak interactions between molecules in each layer, active molecules such as peptides, proteins, nucleic acids, and polysaccharides are loaded, allowing the layers to spontaneously associate to form structurally complete, stable molecular aggregates or supramolecular structures with specific functions. PDRN is an active polynucleotide with a molecular weight range of 50 kDa to 1500 kDa.

[0026] (5) The ginseng root extract used is Polymicro® ginseng root extract, which is purchased from Shaanxi Weimi Biotechnology Co., Ltd. Polymicro® ginseng root extract utilizes low-temperature multiple centrifugation, impurity removal and concentration and other bioengineering techniques to modify and enhance the extracellular vesicles derived from ginseng root extract, further enhancing its efficacy. Extracellular vesicles have a phospholipid bilayer membrane structure similar to the cell membrane, which is easily absorbed and utilized by cells and has an inherent permeability advantage. The drug delivery capacity, stability, solubility and targeting of extracellular vesicles can be adjusted through technical means. This makes the ginseng extract rich in bioactive components such as ginsenosides, which have strong antioxidant capacity, can neutralize free radicals, reduce the damage of oxidative stress to skin cells, can promote skin microcirculation, increase the blood supply to the skin, thereby improving skin metabolism, and help reduce the appearance of wrinkles and fine lines. Ginseng extract can also stimulate collagen synthesis and inhibit collagen degradation, thereby enhancing the elasticity and firmness of the skin. The average particle size of ginseng root extract is between 150 nm and 170 nm.

[0027] The skin repair composition of this invention comprises the following components by weight: 0.1-12 parts sodium hyaluronate, 0.05-0.36 parts cell nutrient solution, 0.001-20 parts super-assembled PDRN, 0.01-10 parts milk vesicles, active ingredients, and water.

[0028] Understandably, the sodium hyaluronate may be 0.1-0.5 parts, the cell nutrient solution may be 0.05-0.11 parts, the superassembled PDRN may be 0.001-15 parts, the milk vesicles may be 0.01-5 parts, and the active ingredient may be 0.4-18 parts. Additionally, it may include 0.06-10 parts of ginseng root extract. The amounts of the above-mentioned raw materials can be any values ​​within the defined range or any combination of two values.

[0029] The cell nutrient solution includes amino acids, vitamins, buffers, and β-glucan. The amino acids include lysine, histidine, arginine, aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, isoleucine, leucine, tyrosine, and phenylalanine.

[0030] The nutrient solution contains vitamins B12 and nicotinamide, and buffers including citric acid and trisodium citrate. The nutrient solution contains the following components by mass: lysine 3%-6%, histidine 2%-4%, arginine 2%-5%, aspartic acid 6%-9%, threonine 1%-4%, serine 10%-15%, glutamic acid 10%-14%, proline 1%-4%, glycine 20%-26%, alanine 3%-6%, valine 2.5%-4%, isoleucine 2%-3.5%, leucine 6%-7.5%, tyrosine 2%-5%, phenylalanine 1.5%-4%, vitamins 0.2%-0.5%, buffers 0.8%-1.2%, and β-glucan 0.3%-0.8%. In addition, to help the cell nutrient solution better nourish cells, minerals such as magnesium sulfate may also be included in the cell nutrient solution.

[0031] The synergistic effect of the above-mentioned cell nutrient solution, super-assembled PDRN and milk vesicles and other active ingredients can comprehensively and quickly repair the sub-healthy state of the skin. It has higher bioavailability and skin permeability, and can precisely act on the deep layers of the skin to repair DNA, promoting rapid and efficient repair of the skin.

[0032] It is understood that the functional ingredients in the above-mentioned raw materials may include one or more of moisturizers and skin-feeling agents. The moisturizer may be a conventional moisturizer in the art, excluding sodium hyaluronate. Exemplarily, it may include one or more of betaine, glycerin, 1,2-pentanediol, allantoin, and dipotassium glycyrrhizate. The skin-feeling agent may be a conventional skin-feeling agent in the art. Exemplarily, it may include at least one of glyceryl glucoside, glycol glucoside, hydrogenated lecithin, and cholesterol. This application does not specifically limit the specific type of use of the moisturizer and skin-feeling agent. The moisturizer may be 0.5-13 parts by weight, and the skin-feeling agent may be 0.01-5 parts.

[0033] The method for preparing the above-mentioned skin repair composition includes the step of preparing the skin repair composition using the above-mentioned ingredients, specifically, the following steps: S1. Mix sodium hyaluronate and purified water thoroughly to obtain phase A. In this step, stirring can be carried out at 80-85℃ at a speed of 600-1000 rpm.

[0034] S2. Add the active ingredient to phase A and mix well to obtain phase B. It is understood that the temperature for this step is 80-85℃, and the mixing can also be carried out at a stirring speed of 600-1000 rpm.

[0035] S3. Add superassembled PDRN, milk vesicles, and cell nutrient solution to phase B, and stir to obtain the composition. In this step, to optimize the activity of each component, the superassembled PDRN, milk vesicles, and cell nutrient solution can be added after phase B has cooled to 40-45°C. The stirring speed in this step can be 600-1000 rpm, and the stirring time can be 20-40 min.

[0036] The repair composition of this application can be used to prepare products comprising the thereof, specifically, the products may include personal care products, and it is understood that the products may also include personal care product-acceptable excipients. As an example, personal care products may include skin care products, and more specifically, skin care products may include lotions, face mask liquids, creams, and / or serums.

[0037] The embodiments of this application will be described in detail below with reference to examples. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of this application. For experimental methods in the following embodiments where specific conditions are not specified, please refer to the guidelines given in this application, or follow experimental manuals or conventional conditions in the art, or follow the conditions recommended by the manufacturer, or refer to experimental methods known in the art.

[0038] In the specific embodiments described below, the measurement parameters involving raw material components may have slight deviations within the weighing accuracy range unless otherwise specified. Temperature and time parameters are subject to acceptable deviations due to instrument testing accuracy or operational precision.

[0039] Example Examples 1-4 Step S1. Add sodium hyaluronate to purified water, heat to 83°C, and stir evenly at 800 rpm to obtain phase A; Step S2. Add the active ingredient to phase A at a temperature of 83°C and stir at a stirring speed of 8000 rpm to obtain phase B; Step S3. After cooling phase B to 40°C, add cell nutrient solution, Polyglycin® super-assembled PDRN, Polyglycin® milk phospholipid vesicle extract, and Polyglycin® ginseng root extract to phase B, and stir at 800 rpm for 30 minutes to obtain the skin repair composition.

[0040] The cell nutrient solution contains the following components by mass: 4% lysine, 3% histidine, 4% arginine, 6% aspartic acid, 3% threonine, 14% serine, 13% glutamic acid, 3% proline, 25% glycine, 4% alanine, 3% valine, 3% isoleucine, 7% leucine, 3% tyrosine, 3% phenylalanine, 0.36% vitamins, 0.94% buffer, 0.5% β-glucan, and 0.2% magnesium sulfate.

[0041] The types and amounts of each substance added in Examples 1-4 are shown in Table 1.

[0042] Table 1. Types and amounts of each substance added in Examples 1-4 (by weight)

[0043] Comparative Example Comparative Examples 1-8 The difference from Example 1 is that the amount of each substance added is different, as detailed in Table 2.

[0044] Table 2. Types and amounts of each substance added in Comparative Examples 1-8 (by weight)

[0045] Comparative Example 9 The difference from Example 1 is that in the cell nutrient solution, lysine is replaced with an equal amount of hydroxyproline, and histidine is replaced with an equal amount of tryptophan.

[0046] Comparative Example 10 The difference from Example 1 is that the cell nutrient solution does not contain β-glucan.

[0047] Comparative Examples 11-15 The difference from Example 1 is that the amount of each substance added is different. The amount of each substance added in Comparative Examples 11-15 is shown in Table 3.

[0048] Table 3. Amounts of each substance added in Comparative Examples 11-15 (by weight)

[0049] Performance testing experiment Experimental Example 1 - Silk fibroin content measurement

[0050] Filiform protein (FLG) is a core molecule in the stratum corneum of the skin that connects keratin fibers, and its content is closely related to the skin's repair ability. The method for testing FLG content is as follows: (1) The test samples were grouped into positive group, negative group and sample group. According to the group, the 3D epidermal skin model with a volume similar to that of the 6-well plate was transferred into the 6-well plate. 0.9mE EpiGrowth culture medium (EpiGrowth culture medium was purchased from Abcam) was added to the 6-well plate in advance. The test group number was marked on the 6-well plate.

[0051] (2) 25 μL of 0.1% sodium lauryl sulfate (SLS) was added to the surface of the 3D epidermal skin model in the 6-well plate containing the positive group, negative group, and sample group, and incubated for 30 min. During this time, the 3D epidermal skin model was stimulated by SLS. After incubation, 100 μg / mL of vitamin C and 7 μg / mL of vitamin E were added to the positive group, with the same amount of vitamin C and vitamin E. The mixture of vitamin C and vitamin E was then evenly applied to the stimulated 3D epidermal skin model in the corresponding control group. In the sample group, any composition prepared in the examples and comparative examples was evenly applied to the surface of the stimulated 3D epidermal skin model. No further treatment was performed on the negative group. Then, the positive group, negative group, and sample group were all placed in a CO2 incubator (37°C, 5% CO2) for 24 h.

[0052] (3) After incubation, the remaining test sample was washed with sterile PBS solution, and the residual liquid was wiped away with sterile cotton swabs. The 3D epidermal skin model culture medium was collected in centrifuge tubes. After collection, the samples used for filaggrin (FLG) content detection were frozen and stored at -80℃. The detection and analysis were performed according to the ELISA kit instructions. The repair efficacy of the test samples was evaluated by detecting changes in FLG content. The results are shown in Table 4.

[0053] Table 4. FLG content in skin models treated with the compositions prepared in the examples and comparative examples Experimental item FLG content (pg / mL) Blank group 1 Negative group 0.2 Positive group 0.8 Example 1 0.76 Example 2 0.74 Example 3 0.72 Example 4 0.75 Comparative Example 1 0.25 Comparative Example 2 0.27 Comparative Example 3 0.28 Comparative Example 4 0.3 Comparative Example 5 0.31 Comparative Example 6 0.36 Comparative Example 7 0.33 Comparative Example 8 0.35 Comparative Example 9 0.39 Comparative Example 10 0.41

[0054] As shown in Table 4, the compositions obtained in Examples 1-4 significantly increased the filaggrin content, indicating that the skin repair compositions prepared according to the formulation of this application can promote filaggrin content and effectively repair aging cells. This is especially true when Poly-Micro® ginseng root extract is added, as it has a synergistic effect with other components. Data from Comparative Examples 1-8 show that the synergistic effect of cell nutrient solution, Poly-Glycoside® super-assembled PDRN, and Poly-Micro® milk phospholipid vesicle extract is significant in increasing filaggrin content and has a good effect on skin repair. When at least one of the cell nutrient solution, Poly-Glycoside® super-assembled PDRN, and Poly-Micro® milk phospholipid vesicles is lacking, the skin repair ability of the prepared composition is poor. It should also be noted that the inventors unexpectedly discovered that the cell nutrient solution formula in this application can effectively work synergistically with the other components to achieve the effect of effectively promoting skin repair. When at least one core component in the cell nutrient solution is omitted or removed, the cell nutrient solution’s ability to nourish cells and work synergistically with other components to restore aging skin will be greatly reduced (see Comparative Examples 9 and 10 for details).

[0055] Experimental Example 2 - Efficacy test experiment By measuring changes in transepidermal water loss rate, skin moisture content, and heme content, changes in skin barrier function can be reflected, thereby verifying the repair efficacy of the product under test.

[0056] According to the inclusion criteria, subjects who met the inclusion criteria were recruited, and the test products were distributed to the subjects. The subjects were divided into 19 groups of 5 people each, and applied one of the products from the example or comparative example products every morning and evening. The results were compared and analyzed after 15 minutes, 14 days and 28 days of continuous use of the product.

[0057] Referring to "T / CAB 0152 2022 Test Methods for Seven Efficacy Items of Cosmetics: Anti-wrinkle, Firming, Moisturizing, Oil Control, Repairing, Nourishing, and Soothing", the TEWL value of the right cheek was measured using a Tewameter™ Hex skin moisture loss meter. Each test site was tested three times, and the average value was taken as the final test value for that site. Data collection times were 0 days, 15 minutes, 14 days, and 28 days. The experimental results are shown in Table 5. The moisture content of the right cheek was measured using a Corneometer CM 825 skin moisture test probe. Each test site was tested three times, and the average value was taken as the final test value for that site. Data collection times were 0 days, 15 minutes, 14 days, and 28 days. The test results are shown in Table 6. The heme E value of the right cheek was measured using a Mexameter MX 18 skin melanin and hemoglobin test probe. Each test site was tested three times, and the average value was taken as the final test value for that site. Data collection times were 0 days, 15 minutes, 14 days, and 28 days. The experimental results are shown in Table 7. It should be noted that AU is the standardized output unit built into Corneometer® CM 825, and EI is the standardized output unit built into Mexameter MX 18. Both AU and EI are dimensionless relative values, and the changes of corresponding indicators at different time points or locations can be directly compared without additional conversion.

[0058] Table 5. TEWL values ​​at the cheek (g / (m²)) 2 ·h))

[0059] Table 6. Measurement values ​​of moisture content in the right cheek

[0060] Table 7. Results of Heme E Value Test

[0061] It should be noted that in Tables 5, 6, and 7, the data for Comparative Examples 4 and 5 are similar to those for Comparative Example 3, and the data for Comparative Examples 6 and 8 are similar to those for Comparative Example 7; therefore, they will not be listed here again. The above data shows that the TEWL, water content, and heme E values ​​of the compositions prepared in the embodiments of this application showed significant differences after use, and these significant differences were already apparent at 15 minutes. In contrast, the corresponding TEWL, water content, and heme E values ​​of the compositions prepared in the comparative examples did not change significantly at 0 days, 15 minutes, 14 days, and 28 days. This indicates that the synergistic effect of cell nutrient solution, polyglycoside® super-assembled PDRN, and polysaccharide® milk phospholipid vesicle extract can reduce TEWL value and skin heme E value, resulting in a highly efficient and rapid repair effect on the skin. It also increases the skin's moisture content and promotes the rapid recovery of moisture in skin with a damaged barrier, thus achieving a rapid repair effect. In particular, the repair performance of the composition is even better when polysaccharide® ginseng root extract is added.

[0062] Performance Test 2: Stability Test of Polyglycin® Superassembled PDRN Samples prepared in Examples 1, 2, and Comparative Examples 11-15 were placed in an accelerating chamber at 50°C and left for one month. The content of polyglucono-Tai® superassembled PDRN was then measured. This test used ultraviolet spectrophotometry to determine the content of polyglucono-Tai® superassembled PDRN. The specific detection method is as follows: (1) Preparation of diphenylamine reagent: Accurately weigh 1g (accurate to 0.01g) of diphenylamine reagent, add 100mL of glacial acetic acid and 3mL of concentrated sulfuric acid, mix well and set aside.

[0063] (2) Preparation of reference solution and test sample solution: Accurately weigh the reference standard of polygluconate® superassembled PDRN raw material, add 0.1M sodium hydroxide solution, and dilute to 1.0 mg / mL to obtain the reference solution; take 1 mL of each of the samples prepared in Example 1, Example 2 and Comparative Examples 11-15, add 0.1M sodium hydroxide solution to each, and dilute to 1.0 mg / mL to obtain the test sample solution.

[0064] (3) Detection: Add the reference solution / sample stock solution, water, diphenylamine reagent, and 2% acetaldehyde solution to the test tube in the following order, and mix well. After the addition is complete, heat in a 60℃ water bath for 60 min. Using water + diphenylamine reagent + 2% acetaldehyde solution as a blank, detect the background solution and sample solution at 595 nm. Perform linear regression on the absorbance of the corresponding solution of the polyacrylamide® superassembled PDRN reference working solution to obtain the linear regression equation. Substitute the absorbance of the sample solution into the linear regression equation to calculate the content and take the average value. Polyacrylamide® superassembled PDRN loss rate = (polyacrylamide® superassembled PDRN content of the initial sample at room temperature - polyacrylamide® superassembled PDRN content of the accelerated sample at 50℃) / polyacrylamide® superassembled PDRN content of the initial sample at room temperature × 100%. The test results are shown in Table 8.

[0065] Table 8. Loss rate of PDRN in the superassembly of Polyglucon® (%) Experimental item Example 1 Example 2 Comparative Example 11 Comparative Example 12 Comparative Example 13 Comparative Example 14 Comparative Example 15 Loss rate (%) 0.97 0.98 10.23 5.45 9.68 5.51 9.59

[0066] As shown in Table 8, the loss rate of polyglycoside® superassembled PDRN in the compositions prepared in Examples 1 and 2 was less than 1%, while the loss rate of polyglycoside® superassembled PDRN in the compositions prepared in Comparative Examples 11-15 was higher. In particular, without the addition of cell nutrient solution and polyglycoside® milk phospholipid vesicle extract, the loss rate of polyglycoside® superassembled PDRN could be as high as 10%. It can be seen that the synergistic effect of cell nutrient solution, polyglycoside® superassembled PDRN and polyglycoside® milk phospholipid vesicles can promote the stability of polyglycoside® superassembled PDRN, so that polyglycoside® superassembled PDRN can exert its function for a long time. Combined with the data in Tables 5, 6 and 7, it can be seen that it is precisely because the cell nutrient solution and polyglycoside® milk phospholipid vesicle extract can promote the stable existence of polyglycoside® superassembled PDRN in this application that it helps polyglycoside® superassembled PDRN to work synergistically with polyglycoside® superassembled PDRN and polyglycoside® milk phospholipid vesicles to jointly promote the efficient and rapid repair of aging skin.

[0067] Furthermore, comparisons of data from Comparative Examples 12 and 13, and Comparative Examples 14 and 15, indicate that the cell nutrient solution itself can, to some extent, stabilize polyacrylamide® superassembled PDRN. This may be because the cell nutrient solution provides nutritional support to cells; its amino acids, vitamins, and polysaccharides maintain normal cellular metabolism and repair mechanisms, indirectly protecting DNA stability. Simultaneously, it regulates the intracellular environment, maintaining acid-base balance and ion concentration to prevent DNA structural damage. However, only when the cell nutrient solution synergistically interacts with polyacrylamide® milk phospholipid vesicle extract can it better promote the stability of polyacrylamide® superassembled PDRN. This may be because the synergistic effect with polyacrylamide® milk phospholipid vesicle extract enhances its ability to encapsulate and protect DNA, promotes bioavailability and absorption, allowing it to more effectively exert its protective effect. It can also activate the cell's own repair mechanisms, promote DNA repair enzyme activity, regulate cell signaling pathways, promptly repair DNA damage, and maintain DNA stability.

[0068] Performance Test III, Safety Evaluation and Satisfaction Evaluation Experimental Example 1 - Safety evaluation

[0069] The safety of the compositions prepared in the examples to human skin was observed through human patch testing, mainly to see if adverse reactions such as erythema, papules, and itching occurred on the subjects' skin. Specifically, referring to the skin reaction grading standards for human occlusive patch testing specified in the 2015 edition of the "Cosmetic Safety Technical Specifications", human occlusive patch testing was conducted on 30 subjects. Taking Example 1 as an example, the evaluation results are shown in Table 9.

[0070] Table 9. Results of human closed patch test Score level Number of cases 0 level 30 cases 1 level 0 cases 2 level 0 cases 3 level 0 cases 4 level 0 cases

[0071] As shown in Table 9, no adverse skin reactions were observed with the skin repair compositions prepared in the embodiments of the present invention. The safety profile is good.

[0072] Experimental Example 2 - Satisfaction evaluation In this test, 10 volunteers were recruited in each group. All of them had sensitive skin. The subjects used a constant amount of the composition prepared in the example every morning and evening. Subjective evaluations were collected after 28 days. Taking Example 1 as an example, the subjective evaluation results after 28 days are shown in Table 10 (average value).

[0073] Table 10. Self-assessment results (satisfaction) of subjects after 28 days of product use.

[0074] Note: Rating criteria: 0-20% is unsatisfactory, 20-40% is somewhat unsatisfactory, 40-60% is average, 60-80% is satisfactory, and 80-100% is very satisfied.

[0075] Analysis of the data in Table 10 shows that the volunteers were very satisfied with the skin repair composition prepared in the examples, and the products prepared in the examples of this application are suitable for widespread use.

[0076] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0077] The embodiments described above are merely illustrative of several implementation methods of this application, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims, and the specification can be used for interpretation.

Claims

1. A skin repair composition, characterized in that, By weight, it comprises the following components: 0.1-12 parts sodium hyaluronate, 0.05-0.36 parts cell nutrient solution, 0.001-20 parts super-assembled PDRN, 0.01-10 parts milk vesicles, active ingredients, and water; wherein the cell nutrient solution comprises amino acids, vitamins, buffers, and β-glucan.

2. The composition according to claim 1, characterized in that, The amino acids include lysine, histidine, arginine, aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, isoleucine, leucine, tyrosine, and phenylalanine.

3. The composition according to claim 1, characterized in that, It also includes 0.06-10 parts by weight of ginseng root extract.

4. The composition according to claim 1, characterized in that, By weight, the sodium hyaluronate is 0.1-0.5 parts, the cell nutrient solution is 0.05-0.11 parts, the super-assembled PDRN is 0.001-15 parts, and the milk vesicles are 0.01-5 parts.

5. The composition according to claim 1, characterized in that, One or more of the following conditions must be met: (a) The active ingredients include skin feelers and / or moisturizers; (b) The effective ingredient is 0.4-18 parts by weight; (c) The buffer comprises citric acid and trisodium citrate; (d) The vitamins mentioned include vitamin B12 and nicotinamide.

6. The composition according to claim 5, characterized in that, One or more of the following conditions must be met: (e) The humectant includes one or more of betaine, glycerin, 1,2-pentanediol, allantoin and dipotassium glycyrrhizate; (f) The skin-feeling agent includes one or more of glyceryl glucoside, glycol glucoside, hydrogenated lecithin, and cholesterol; (g) By weight, the moisturizer is 0.5-13 parts and the skin feeler is 0.01-5 parts.

7. The method for preparing the composition according to any one of claims 1-6, characterized in that, Includes the following steps: S1. Mix sodium hyaluronate and water to obtain phase A; S2. Add the active ingredient to phase A to obtain phase B; S3. Add superassembled PDRN, milk vesicles and cell nutrient solution to phase B, stir, and obtain the composition.

8. The method for preparing the composition according to claim 7, characterized in that, One or more of the following conditions must be met: (h) In step S1, stir at 80-85℃ at a speed of 600-1000 rpm; (i) In step S3, after phase B cools down to 40-45°C, add superassembled PDRN, milk vesicles and cell nutrient solution; (j) In step S3, the stirring speed is 600-1000 rpm and the stirring time is 20-40 min; (k) In step S3, ginseng root extract is also added to phase B.

9. A product characterized in that, Includes the skin repair composition according to any one of claims 1-6 or the repair composition prepared by any one of claims 7-8; Preferably, the product includes personal care products; more preferably, the product also includes personal care product-acceptable excipients; even more preferably, the personal care products include skin care products.

10. The product according to claim 9, characterized in that, The skincare products include lotions, face masks, creams, and / or serums.

Images