Nucleic acid automatic extraction device and process thereof

Abstract

The application provides a nucleic acid automatic extraction device and a process thereof. The device comprises a body, a detection platform and an extraction part. The upper part of the body is provided with a first displacement mechanism, which is connected with the extraction part. The detection platform is slidably arranged at the bottom of the body and matches the work of the extraction part. The extraction part comprises a magnetic bar sleeve assembly for stirring extraction liquid and a magnetic bar assembly for adsorbing magnetic beads, and the magnetic bar sleeve assembly is arranged at the bottom of the magnetic bar assembly. The magnetic bar assembly comprises fixed magnetic bars, movable magnetic bars and round rods. A plurality of fixed magnetic bars are fixedly arranged at the lower part of a first bottom plate. The first bottom plate is arranged in the horizontal direction and is fixedly connected with the first displacement mechanism. The round rods are inserted into the interiors of the fixed magnetic bars, and the bottoms of the round rods are provided with movable inner grooves. The movable magnetic bars can move up and down along the movable inner grooves and are attracted to or separated from the fixed magnetic bars. The magnetic bar assembly and the magnetic bar sleeve assembly are high-precision aligned to avoid collision damage, and the stirring mode and the adsorption mode can be switched.

Description

Technical Field

[0001] This invention belongs to the technical field of biotechnology equipment, and specifically relates to an automated nucleic acid extraction device and its process. Background Technology

[0002] Nucleic acid extraction is a crucial pretreatment step in molecular biology detection, and its efficiency and quality directly affect the accuracy of subsequent analyses. Automated nucleic acid extraction equipment based on magnetic beads has become mainstream in the market due to its advantages such as high throughput, automation, and avoidance of harmful reagents. This type of equipment typically uses a combination of magnetic rods and magnetic rod sleeves to adsorb, transfer, and release nucleic acid-bound magnetic beads, thereby achieving nucleic acid separation and purification.

[0003] In existing magnetic rod extraction equipment, the magnetic rod is usually fixedly installed, requiring extremely high alignment precision between it and the magnetic rod sleeve. Any deviation can easily lead to mechanical collisions during the insertion or removal of the magnetic rod from the sleeve. These collisions not only generate noise and wear on parts, but more seriously, they can disturb or damage the adsorbed magnetic bead-nucleic acid complex, resulting in decreased extraction efficiency, sample cross-contamination, or even extraction failure. Furthermore, traditional magnetic rods have a limited function in the mixing stage, only moving the liquid through the movement of the sleeve, with limited agitation capabilities for the magnetic beads. This is particularly problematic in high-viscosity or complex samples, potentially leading to insufficient binding of the magnetic beads to nucleic acids, making mixing efficiency the bottleneck of the entire extraction process.

[0004] Therefore, there is an urgent need in this field for an automated nucleic acid extraction solution that can solve the above problems, namely, to ensure high-precision alignment to avoid collision damage, while effectively improving the mixing efficiency, thereby ensuring the high efficiency and reliability of nucleic acid extraction. Summary of the Invention

[0005] In order to solve the above-mentioned problems in the prior art, this application provides an automated nucleic acid extraction device and process to solve the above-mentioned technical defects.

[0006] According to a first aspect of the present invention, an automated nucleic acid extraction device is provided, comprising: a body, a detection platform, and an extraction section. A first displacement mechanism is provided on the upper part of the body and is connected to the extraction section. The detection platform is slidably disposed at the bottom of the body and works in conjunction with the extraction section. The extraction section includes a magnetic rod sleeve assembly for stirring the extraction liquid and a magnetic rod assembly for adsorbing magnetic beads, with the magnetic rod sleeve assembly disposed at the bottom of the magnetic rod assembly. The magnetic rod assembly includes a fixed magnetic rod, a movable magnetic rod, and a round rod. Multiple sets of fixed magnetic rods are fixedly disposed on the lower part of a first base plate, which is horizontally disposed and fixedly connected to the first displacement mechanism. The round rod is inserted into the interior of the fixed magnetic rod, and a movable inner groove is provided at the bottom of the round rod. The movable magnetic rod can move up and down along the movable inner groove, engaging or separating from the fixed magnetic rod.

[0007] In this structural design, a magnetic rod assembly consisting of a fixed magnetic rod and a movable magnetic rod that can move up and down works in tandem to achieve switching between stirring and adsorption functions. When the device switches to stirring mode, the movable magnetic rod can move downwards, and its independently generated magnetic field works in conjunction with the magnetic rod sleeve to more violently agitate the magnetic beads, significantly improving the contact efficiency and binding rate between the magnetic beads and nucleic acids. When the device switches to adsorption mode, the movable and fixed magnetic rods are tightly attracted to each other, forming a high-intensity, high-gradient composite magnetic field, which enables rapid and complete capture and fixation of the magnetic beads, effectively preventing loss during the transfer process and ensuring a high recovery rate of nucleic acids.

[0008] In a specific embodiment, the device further includes an adjustment plate, which is mounted on a first base plate. The adjustment plate and the first base plate are provided with a plurality of limiting holes that match the round rods. The multiple round rods pass through the limiting holes from bottom to top through the first base plate and the adjustment plate.

[0009] This structural design ensures that the multiple round rods maintain precise parallelism and relative position as they pass through the first base plate and the adjusting plate, enabling all moving magnetic rods to move synchronously and stably up and down. This not only effectively prevents a single round rod from tilting or getting stuck during movement and ensures the reliability of the magnetic rod assembly when switching between stirring and adsorption modes, but also simplifies the assembly process through modular design, facilitating subsequent maintenance and component replacement.

[0010] In a specific embodiment, the device also includes a limiting frame plate. The surface of the limiting frame plate is provided with multiple sets of limiting parts. One end of the round rod that passes through the adjusting plate is provided with a limiting groove, and the limiting groove and the limiting part are nested together.

[0011] Through this structural design, the nested engagement of the limiting groove at the upper end of the round rod with the limiting frame plate provides crucial top constraint for the magnetic rod assembly. Together with the adjusting plate at the bottom, it forms a stable motion frame, effectively improving the rigidity and stability of the entire assembly during high-speed reciprocating motion.

[0012] In a specific embodiment, the adjustment plate is provided with at least one slide bar, the extension direction of the slide bar is parallel to the limiting part, and the middle part of the slide bar is provided with a limiting protrusion. The limiting frame plate can slide to both sides of the slide bar with the limiting protrusion as the boundary.

[0013] In this structural design, the limiting protrusion restricts the limiting plate to slide only to both sides, which helps prevent the limiting plate from disengaging from the slide bar after sliding to one side. The slide bar cooperates with the limiting protrusion to provide a reliable guiding function for the movement of the limiting plate, ensuring the accuracy and reliability of its locking and unlocking actions with the magnetic rod assembly.

[0014] In a specific embodiment, a reset spring is installed inside the movable inner groove. One end of the reset spring is connected to the round rod, and the other end is connected to the movable magnetic rod.

[0015] In this structural design, the reset spring within the movable inner groove enables flexible contact and automatic reset between the movable magnetic rod and the magnetic rod sleeve. When the bottom of the movable magnetic rod contacts the magnetic rod sleeve or the bottom of the test tube, the reset spring effectively absorbs impact energy, acting as a buffer to prevent noise and component damage caused by hard collisions. After the contact force is eliminated, the reset spring drives the movable magnetic rod to automatically return to its initial position, ensuring the accuracy and reliability of its alignment with the fixed magnetic rod in subsequent adsorption and transfer steps, thereby improving the stability and lifespan of the equipment.

[0016] In a specific embodiment, the device further includes a second displacement mechanism, which works in conjunction with the adjustment plate to drive the movable magnetic rod to move up and down.

[0017] This structural design enables automated and precise control of the lifting and lowering motion of the movable magnetic rod, ensuring the reliability of working mode switching and the continuity of the entire extraction process, and significantly improving the automation level of the equipment.

[0018] In a specific embodiment, both the fixed magnetic rod and the movable magnetic rod are permanent magnets, and the magnetic poles of the fixed magnetic rod and the movable magnetic rod are arranged to attract each other.

[0019] This structural design allows the movable magnetic rod to automatically and accurately align and tightly engage with the fixed magnetic rod under magnetic attraction when resetting; furthermore, the two work together as a high-strength composite magnet in adsorption mode, significantly enhancing the magnetic bead capture capability.

[0020] In a specific embodiment, the magnetic rod sleeve assembly includes multiple magnetic rod sleeves that correspond one-to-one with the movable magnetic rod, and the movable magnetic rod can extend into the corresponding magnetic rod sleeve.

[0021] In this structural setup, multiple parallel and independent nucleic acid extraction units are constructed by setting up multiple magnetic rod sleeves, each corresponding one-to-one with a movable magnetic rod. This design enables the device to process multiple samples simultaneously, significantly improving extraction efficiency and throughput. Each working unit (movable magnetic rod and its corresponding sleeve) can independently and precisely complete stirring and adsorption operations, effectively preventing cross-contamination between samples. Furthermore, the one-to-one correspondence ensures that the actuator can precisely and synchronously control the displacement of each working unit, guaranteeing the coordination and consistency of all magnetic rod sleeves and movable magnetic rods in complex action sequences, thereby achieving stable and reliable automated extraction under high-throughput conditions.

[0022] In a specific embodiment, the device further includes a second base plate, which is vertically installed inside the machine body and has a rectangular window; the extraction part is attached to the second base plate and moves up and down along the rectangular window; the surface of the second base plate is also provided with disinfection lamps, which are respectively installed on both sides of the extraction part.

[0023] This structural design provides physical isolation and automatic ultraviolet disinfection capabilities for the working area of ​​the extraction section, effectively preventing cross-contamination of the equipment interior and between samples by aerosols, and improving the biosafety and automated maintenance level of the equipment.

[0024] According to a second aspect of the present invention, an automated nucleic acid extraction process is also provided, implemented by the aforementioned automated nucleic acid extraction device, comprising:

[0025] S1: Place the nucleic acid test tube to be tested and the empty test tube on the surface of the detection platform. The detection platform moves to the working area of ​​the extraction section and aligns with the magnetic rod sleeve assembly. The magnetic rod sleeve assembly moves into the nucleic acid test tube to be tested under the drive of the first displacement mechanism and moves up and down to stir.

[0026] S2: The adjusting plate moves downward under the drive of the second displacement mechanism, the movable magnetic rod separates from the fixed magnetic rod and extends into the bottom of the nucleic acid test tube to be tested, and the movable magnetic rod moves up and down synchronously with the magnetic rod sleeve assembly under the drive of the second displacement mechanism.

[0027] S3: The adjusting plate is reset under the drive of the second displacement mechanism, the movable magnetic rod and the fixed magnetic rod are attracted together, the magnetic rod assembly is placed inside the magnetic rod sleeve assembly, and the nucleic acid is adsorbed.

[0028] S4: Transfer the adsorbed magnetic beads into an empty test tube, separate the magnetic rod assembly from the magnetic rod sleeve assembly, and let the magnetic beads fall into the empty test tube for testing.

[0029] In this process design, a dual stirring mechanism combining "magnetic rod sleeve mechanical stirring" and "moving magnetic rod magnetic field disturbance" is used, along with dynamic magnetic field control achieved through "separation and attraction between moving and fixed magnetic rods". This fully automated process simultaneously achieves extremely high nucleic acid extraction efficiency, recovery rate, and purity, avoiding the problems of insufficient mixing, incomplete adsorption, and easy cross-contamination in traditional processes.

[0030] Compared with the prior art, the beneficial results of the present invention are as follows:

[0031] 1. This equipment drives the adjusting plate through a second displacement mechanism, which can change the gap between the movable and fixed magnetic rods, thereby switching between stirring and adsorption modes. It employs a dual mixing mechanism combining "magnetic rod sleeve mechanical stirring" and "movable magnetic rod magnetic field disturbance," effectively solving the inherent problems of easy precipitation and insufficient mixing of magnetic beads in traditional methods, and greatly improving the binding efficiency of magnetic beads and nucleic acids. Simultaneously, utilizing the combined strong magnetic field formed by the attraction of the movable and fixed magnetic rods during adsorption, it achieves near-complete and rapid capture and enrichment of magnetic beads, thus fundamentally ensuring high nucleic acid recovery rate and high extraction purity.

[0032] 2. A reset spring is installed inside the movable magnetic rod, which realizes the flexible contact and automatic reset function of the movable magnetic rod. This helps to effectively absorb the impact energy generated by the movable magnetic rod during high-speed operation, significantly reduce the operating noise of the equipment, and at the same time avoid wear and damage to the movable magnetic rod caused by rigid collision.

[0033] 3. The combination of the limiting plate and the adjusting plate allows multiple rods to be quickly disassembled by pulling the limiting plate to both sides. At the same time, the clamping strip provides auxiliary limiting to prevent the rods from falling off at the same time and causing damage to the equipment. Attached Figure Description

[0034] The accompanying drawings are included to provide a further understanding of the embodiments and are incorporated in and constitute a part of this specification. The drawings illustrate embodiments and, together with the description, serve to explain the principles of the invention. Other embodiments and many anticipated advantages of the embodiments will be readily recognized as they become better understood through reference to the following detailed description. Elements in the drawings are not necessarily to scale. The same reference numerals refer to corresponding similar parts.

[0035] Figure 1 This is a schematic diagram of the structure of an automated nucleic acid extraction device according to an embodiment of the present invention;

[0036] Figure 2 This is an assembly diagram of the extraction unit according to an embodiment of the present invention;

[0037] Figure 3 This is an assembly diagram of the first driver according to an embodiment of the present invention;

[0038] Figure 4 This is an assembly diagram of the magnetic rod assembly according to an embodiment of the present invention;

[0039] Figure 5 (a) is a structural schematic diagram of the limiting frame plate according to an embodiment of the present invention;

[0040] Figure 5 (b) is a schematic diagram of the structure of the adjustment plate according to an embodiment of the present invention;

[0041] Figure 6 This is a schematic diagram of the magnetic rod assembly according to an embodiment of the present invention;

[0042] Figure 7 This is a schematic diagram of the structure of the magnetic rod assembly and the magnetic rod sleeve embedded in an embodiment of the present invention.

[0043] The meanings of the numbers in the diagram are as follows: 1. Body; 2. Extraction section; 3. Detection platform; 4. Control panel; 5. Door; 11. Sliding section; 12. First actuator; 13. Support plate; 14. Second actuator; 15. Sliding column; 16. First base plate; 17. First magnetic rod; 18. Second magnetic rod; 121. Frame; 122. Limiting plate; 123. Locking bar; 124. Locking plate; 125. Movable groove; 126. Tension spring; 127. 131. Adjustment plate; 132. Round rod; 133. First limiting groove; 134. Second limiting groove; 135. Movable inner groove; 136. Third magnetic rod; 137. Return spring; 21. Magnetic rod assembly; 22. Magnetic rod sleeve assembly; 23. Slide rail; 24. Second base plate; 25. Disinfection lamp; 211. Stand; 212. Limiting frame plate; 271. Plate body; 272. Limiting hole; 273. Sliding sleeve; 274. Central column; 275. Sliding bar. Detailed Implementation

[0044] In the following detailed description, reference is made to the accompanying drawings, which form part of the detailed description and are illustrated by specific illustrative embodiments in which the invention may be practiced. In this regard, directional terms such as “top,” “bottom,” “left,” “right,” “up,” “down,” etc., are used with reference to the orientation of the described figures. Because components of the embodiments can be positioned in several different orientations, directional terms are used for illustrative purposes and are by no means limiting. It should be understood that other embodiments may be utilized or logical changes may be made without departing from the scope of the invention. Therefore, the following detailed description should not be taken in a limiting sense, and the scope of the invention is defined by the appended claims.

[0045] This invention proposes an automated nucleic acid extraction device. Figure 1 A schematic diagram of the structure of an automated nucleic acid extraction device according to the first embodiment of the present invention is shown, as follows: Figure 1 As shown, an automated nucleic acid extraction device includes: a body 1, an extraction section 2, a detection platform 3, a control panel 4, and a door 5. The extraction section 2 is located on the upper part of the body 1. The detection platform 3 is slidably located on the bottom of the body 1 and works in conjunction with the extraction section 2. The control panel 4 is located on the surface of the body 1 and is used to control the movement of the detection platform 3. The door 5 is closable located at the front end of the body 1.

[0046] In this structure, the extraction unit 2 is mainly used for stirring, adsorption and extraction of the nucleic acid to be tested; the surface of the detection platform 3 can be used to install the nucleic acid sample to be tested and the test tube to be extracted and contained; the control panel 4 can adjust the stirring frequency of the extraction unit 2 inside the machine body 1 and the direction of the horizontal movement of the detection platform 3; the door 5 is kept closed when the equipment is working to isolate external environmental pollution, and is opened after the equipment is finished working to facilitate the removal of the nucleic acid sample.

[0047] Figure 2 An assembly schematic diagram of the extraction unit according to an embodiment of the present invention is shown. Figure 2 As shown, the second base plate 24 is vertically erected and has a rectangular window; a slide rail 23 is installed in the middle of the second base plate 24, and the slide rail 23 is located outside the rectangular window; the magnetic rod assembly 21 and the magnetic rod sleeve assembly 22 are attached to the second base plate 24 and connected to the first displacement mechanism, and move up and down along the slide rail 23 outside the rectangular window under the drive of the first displacement mechanism. In this example, the magnetic rod assembly 21 and the magnetic rod sleeve assembly 22 are... Figure 1 The extraction section 2 includes a magnetic rod sleeve assembly 22 for stirring the extract, a magnetic rod assembly 21 for adsorbing magnetic beads, and the magnetic rod sleeve assembly 22 is located at the bottom of the magnetic rod assembly 21. In this structural configuration, the rectangular window provides operating space for the first displacement mechanism to move up and down along the slide rail 23, while the height of the rectangular window also limits the first displacement mechanism to move up and down within a safe range.

[0048] Figure 3 An assembly schematic diagram of the first driver according to an embodiment of the present invention is shown. Figure 2 and Figure 3 As shown, the first displacement mechanism includes a sliding part 11 and a first driver 12. The sliding part 11 is mounted on the slide rail 23, and the first driver 12 is provided on the side of the sliding part 11. The external gear of the first driver 12 engages with the rack of the slide rail 23. With this structure, the magnetic rod assembly 21 and the magnetic rod sleeve assembly 22 can slide up and down along the slide rail 23 under the drive of the first displacement mechanism.

[0049] In a specific embodiment, the surface of the second base plate 24 is also provided with disinfection lamps 25, which are respectively disposed on both sides of the magnetic rod assembly 21 and the magnetic rod sleeve assembly 22. This structural arrangement provides physical isolation and automatic ultraviolet disinfection capability for the working area of ​​the extraction section, effectively preventing cross-contamination of the equipment interior and between samples by aerosols, and improving the biosafety and automated maintenance level of the equipment.

[0050] like Figure 3As shown, the magnetic rod assembly includes fixed magnetic rods. Multiple sets of fixed magnetic rods are fixedly disposed on the lower part of the first base plate 16. The fixed magnetic rods include a first magnetic rod 17 and a second magnetic rod 18. The second magnetic rod 18 is disposed at the bottom of the first magnetic rod 17, and the first magnetic rod 17 and the second magnetic rod 18 are fixedly connected by adhesive. The first base plate 16 is arranged horizontally and is fixedly connected to the first displacement mechanism. Four sliding columns 15 are mounted on the surface of the first base plate 16, vertically disposed at the bottom of the support plate 13 to support the support plate 13. A second displacement mechanism is also mounted on the upper middle part of the support plate 13. In this example, the second displacement mechanism is a second actuator 14. In this structural configuration, the rigid connection between the first base plate 16 and the second actuator 14 ensures the overall stability of the magnetic rod assembly 21's movement; the cooperation between the sliding column 15 and the support plate 13 forms a stable motion guiding frame, enabling the second actuator 14 to precisely control the lifting and lowering movement of the magnetic rod assembly 21, thereby maintaining extremely high positioning accuracy and motion synchronization when switching between stirring and adsorption modes, greatly improving the efficiency and reliability of nucleic acid extraction.

[0051] Figure 4 An assembly schematic diagram of a magnetic rod assembly according to an embodiment of the present invention is shown. Figure 5 (a) and Figure 5 (b) shows a schematic diagram of the structure of the limiting frame plate and the adjusting plate according to an embodiment of the present invention, as follows: Figure 3-5 As shown, the device also includes an adjustment plate 127, which is mounted on the first base plate. The plate body 271 of the adjustment plate 127 and the first base plate are provided with a plurality of limiting holes 272 that match the round rod. The round rod passes through the first base plate and the adjustment plate 127 from bottom to top through the limiting holes 272.

[0052] This structural design ensures that the circular rod maintains precise parallelism and relative position as it passes through the first base plate and the adjusting plate 127, enabling all movable magnetic rods to move synchronously and stably up and down. This not only effectively prevents individual movable magnetic rods from skewing or getting stuck during movement, but also simplifies the assembly process through modular design, facilitating subsequent maintenance and component replacement.

[0053] In a specific embodiment, four sliding sleeves 273 are also provided on the surface of the plate 271, and the plate 271 is connected to the sliding sleeves 273. Figure 3 With the sliding column 15 in the middle, when the second driver rotates and drives the central column 274 in the middle of the plate 271, the plate 271 moves up and down with the assistance of the sliding sleeve 273, so that the movable magnetic rod can move up and down, thus participating in the work when the equipment is stirring, making the stirring more uniform.

[0054] like Figure 4 , Figure 5As shown, the device also includes a limiting frame plate 212. The surface of the limiting frame plate 212 is provided with multiple sets of limiting parts. One end of the round rod that passes through the adjusting plate 127 is provided with a limiting groove, and the limiting part is nested and cooperated with the limiting part through the limiting groove. The limiting part includes a grid-like structure formed by the frame body 121 and the locking plate 124. The frame body 121 of the limiting frame plate 212 is engaged with the movable magnetic rod through the locking plate 124. The frame bodies 121 are symmetrically arranged. The frame body 121 is pulled by the tension spring 126 installed inside the movable groove 125, so that the locking plate 124 opposite the frame body 121 locks the movable magnetic rod. The movable magnetic rod can be unlocked by pulling one side of the frame body 121 outward. When the frame body 121 slides sideways, it is limited by the slide bar 275 on the surface of the plate body 271. The frame body 121 slides on the inner surface of the slide bar 275. In this structural design, the grid-like layout formed by the frame 121 and the locking plate 124, combined with the automatic rebound of the tension spring 126 and the precise guidance of the slide bar 275, enables the synchronous, rapid locking and release of multiple movable magnetic rods.

[0055] In a specific embodiment, a limiting horizontal plate 122 is also provided on the surface of the frame 121, and a retaining strip 123 is provided inside the limiting horizontal plate 122. The retaining strip 123 is made of silicone. When the frame 121 slides, the retaining strip 123 is embedded in the second limiting groove 133 of the round rod 131 to further limit the movement of the magnetic rod. With this structural design, the demagnetization and detachment of multiple movable magnetic rods can be avoided when the frame 121 unlocks simultaneously, and replacement is also more convenient.

[0056] In a specific embodiment, the sliding bar 275 extends parallel to the limiting portion, and a limiting protrusion is provided in the middle of the sliding bar 275. The limiting plate 212 can slide to both sides of the sliding bar 275 with the limiting protrusion as the boundary. In this structural arrangement, the limiting protrusion restricts the limiting plate 212 to slide only to both sides, which helps to prevent the limiting plate 212 from detaching from the sliding bar 275 after sliding to one side.

[0057] Figure 6A schematic diagram of the magnetic rod assembly according to an embodiment of the present invention is shown. The magnetic rod assembly 21 includes a fixed magnetic rod, a movable magnetic rod, and a round rod 131. In this example, the fixed magnetic rod is a first magnetic rod 17 and a second magnetic rod 18, and the movable magnetic rod is a third magnetic rod 135. The round rod 131 is inserted into the second magnetic rod 18, and a movable inner groove 134 is provided at the bottom of the round rod 131. The third magnetic rod 135, which can move up and down, is installed inside the movable inner groove 134, and a movable rod is provided at the top of the third magnetic rod 135 to cooperate with the movable inner groove 134. A return spring 136 is provided between the movable rod and the movable inner groove 134. The return spring 136 can buffer the third magnetic rod 135 against the bottom surface of the magnetic rod sleeve assembly when it enters the magnetic rod sleeve assembly, avoiding damage caused by impact. The bottom of the third magnetic rod 135 is chamfered, which allows for fine adjustment of the small gap with the movable rod during alignment, making it less likely to cause hard impact when entering the magnetic rod sleeve assembly. In this structural configuration, the second magnetic rod 18 and the vertically movable third magnetic rod 135 work together to achieve switching between stirring and adsorption functions: when the device switches to stirring function, the third magnetic rod 135 can move downwards, and its independently generated magnetic field works in conjunction with the magnetic rod sleeve assembly to more violently agitate the magnetic beads, significantly improving the contact efficiency and binding rate between the magnetic beads and nucleic acids; when the device switches to adsorption function, the third magnetic rod 135 and the second magnetic rod 18 are tightly attracted to each other, forming a high-intensity, high-gradient composite magnetic field, which enables rapid and complete capture and fixation of the magnetic beads, effectively preventing loss during the transfer process and ensuring a high recovery rate of nucleic acids.

[0058] In a specific embodiment, the top of the round rod 131 is provided with a first limiting groove 132 and a second limiting groove 133, which are nested and engaged with the limiting frame plate. This structural design provides crucial top constraint for the magnetic rod assembly, forming a stable motion frame together with the bottom adjustment plate, effectively improving the rigidity and stability of the entire assembly during high-speed reciprocating motion.

[0059] In a specific embodiment, both the second magnetic rod 18 and the third magnetic rod 135 are permanent magnets, and their magnetic poles are arranged in an attractive configuration. This structural arrangement allows the third magnetic rod 135 to automatically and precisely align and tightly engage with the second magnetic rod 18 under magnetic attraction during resetting. Furthermore, the two work collaboratively as a high-strength composite magnet in the adsorption mode, significantly enhancing the magnetic bead's capture capability.

[0060] Figure 7 A schematic diagram of the structure of the magnetic rod assembly and the magnetic rod sleeve embedded according to an embodiment of the present invention is shown. Figure 7As shown, the magnetic rod sleeve assembly 22 includes multiple magnetic rod sleeves, each corresponding to a movable magnetic rod, and the movable magnetic rod can extend into its corresponding sleeve. In this structural configuration, multiple parallel and independent nucleic acid extraction units are constructed by setting up multiple magnetic rod sleeves corresponding to the movable magnetic rods. This design enables the device to process multiple samples simultaneously, significantly improving extraction efficiency and throughput. Each unit (movable magnetic rod and its corresponding sleeve) can independently and precisely complete stirring and adsorption operations, effectively preventing cross-contamination between samples. Furthermore, the one-to-one correspondence ensures that the second actuator 14 can precisely and synchronously control the displacement of each unit, guaranteeing the coordinated consistency of all magnetic rod sleeves and movable magnetic rods in complex action sequences, thereby achieving stable and reliable automated extraction under high-throughput conditions.

[0061] According to a second aspect of the present invention, an automated nucleic acid extraction process is also provided, implemented by the aforementioned automated nucleic acid extraction device, comprising:

[0062] S1: Place the nucleic acid test tube and empty test tube on the surface of the testing platform, flip the door 5 on the surface of the machine, and heat the testing platform to 56℃~65℃;

[0063] S2: The detection platform moves to the working area of ​​the extraction section and aligns with the magnetic rod sleeve assembly. The magnetic rod sleeve assembly moves into the test tube of the nucleic acid to be detected under the drive of the first driver and moves up and down to stir.

[0064] S3: The adjustment plate moves downward under the drive of the second driver, the second and third magnetic rods separate and extend into the bottom of the nucleic acid test tube to be tested, and the third magnetic rod moves up and down synchronously with the magnetic rod sleeve assembly under the drive of the second driver.

[0065] S4: The adjustment plate is reset under the drive of the second driver, the second and third magnetic rods are attracted, the magnetic rod assembly is placed inside the magnetic rod sleeve assembly, and the nucleic acid is adsorbed.

[0066] S5: Transfer the adsorbed magnetic beads into an empty test tube, separate the magnetic rod assembly from the magnetic rod sleeve assembly, and let the magnetic beads fall into the empty test tube for testing.

[0067] In this process design, a dual stirring mechanism combining "magnetic rod sleeve mechanical stirring" and "moving magnetic rod magnetic field disturbance" is used, along with dynamic magnetic field control achieved through "moving / fixed magnetic rod separation-attraction". This fully automated process simultaneously achieves extremely high nucleic acid extraction efficiency, recovery rate and purity, avoiding the problems of insufficient mixing, incomplete adsorption and easy cross-contamination in traditional processes.

[0068] It is evident that those skilled in the art can make various modifications and alterations to the embodiments of the present invention without departing from the spirit and scope of the invention. In this way, the invention is also intended to cover such modifications and alterations if they fall within the scope of the claims and their equivalents. The word "comprising" does not exclude the presence of other elements or steps not listed in the claims. The simple fact that certain measures are described in mutually different dependent claims does not indicate that a combination of these measures cannot be used for profit. Any reference numerals in the claims should not be considered as limiting the scope.

Claims

1. An automated nucleic acid extraction device, characterized in that, include: The device comprises a body, a detection platform, and an extraction section. The upper part of the body is equipped with a first displacement mechanism connected to the extraction section. The detection platform is slidably disposed at the bottom of the body and works in conjunction with the extraction section. The extraction section includes a magnetic rod sleeve assembly for stirring the extract and a magnetic rod assembly for adsorbing magnetic beads, with the magnetic rod sleeve assembly disposed at the bottom of the magnetic rod assembly. The magnetic rod assembly includes a fixed magnetic rod, a movable magnetic rod, and a round rod. Multiple sets of fixed magnetic rods are fixedly disposed at the lower part of a first base plate, which is horizontally oriented and fixedly connected to the first displacement mechanism. The round rod is inserted into the interior of the fixed magnetic rod, and its bottom has a movable inner groove. The movable magnetic rod can move up and down along the movable inner groove, engaging or disengaging with the fixed magnetic rod. It also includes an adjusting plate, which is mounted on the first base plate. The adjusting plate and the first base plate are provided with multiple limiting holes corresponding to the round rods. Multiple round rods pass through the limiting holes sequentially from bottom to top through the first base plate and the adjusting plate. It also includes a limiting frame plate, the surface of which is provided with multiple sets of limiting parts. One end of each round rod extending from the adjusting plate has a limiting groove, and the limiting groove and limiting part are nested together. A return spring is installed in the movable inner groove, one end of which is connected to the round rod, and the other end is connected to the movable magnetic rod. It also includes a second displacement mechanism, which works in conjunction with the adjusting plate to drive the movable magnetic rod to move up and down. Both the fixed magnetic rod and the movable magnetic rod are permanent magnets, and their magnetic poles are arranged in an attractive configuration.

2. The automated nucleic acid extraction device according to claim 1, characterized in that, The adjusting plate is provided with at least one slide bar, the extension direction of the slide bar is parallel to the limiting part, and the middle part of the slide bar is provided with a limiting protrusion. The limiting frame plate can slide to both sides of the slide bar with the limiting protrusion as the boundary.

3. The automated nucleic acid extraction device according to claim 1, characterized in that, The magnetic rod sleeve assembly includes multiple magnetic rod sleeves that correspond one-to-one with the movable magnetic rod, and the movable magnetic rod can extend into the corresponding magnetic rod sleeve.

4. The automated nucleic acid extraction device according to claim 1, characterized in that, It also includes a second base plate, which is vertically installed inside the machine body and has a rectangular window; the extraction part is attached to the second base plate and moves up and down along the rectangular window; the surface of the second base plate is also provided with disinfection lamps, which are respectively arranged on both sides of the extraction part.

5. An automated nucleic acid extraction process, characterized in that, The automated nucleic acid extraction device according to any one of claims 1-4 comprises: S1: Place the nucleic acid test tube to be tested and the empty test tube on the surface of the detection platform. The detection platform moves to the working area of ​​the extraction section and aligns with the magnetic rod sleeve assembly. The magnetic rod sleeve assembly moves into the inside of the nucleic acid test tube to be tested under the drive of the first displacement mechanism and moves up and down to stir. S2: The adjusting plate moves downward under the drive of the second displacement mechanism, the movable magnetic rod separates from the fixed magnetic rod and extends into the bottom of the nucleic acid test tube to be tested, and the movable magnetic rod moves up and down synchronously with the magnetic rod sleeve assembly under the drive of the second displacement mechanism; S3: The adjusting plate is reset under the drive of the second displacement mechanism, the movable magnetic rod is attracted to the fixed magnetic rod, and the magnetic rod assembly is placed inside the magnetic rod sleeve assembly to adsorb nucleic acid; S4: Transfer the adsorbed magnetic beads into the empty test tube, separate the magnetic rod assembly from the magnetic rod sleeve assembly, and let the magnetic beads fall into the empty test tube for testing.

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