Nucleic acid detection card box

Abstract

The embodiment of the invention relates to the technical field of in-vitro medical diagnosis devices, and discloses a nucleic acid detection card box, a plurality of cavities and at least one air hole are formed in the card box, the air hole is communicated with the cavities, and the air hole is used for driving fluid in the card box to flow among the cavities when positive pressure or negative pressure is introduced. In addition, a water-absorbing breathable film is arranged between the air hole and the cavity communicated with the air hole, and the water-absorbing breathable film is used for preventing water from entering the card box and eliminating bubbles. By means of the mode, on one hand, external moisture can be prevented from entering the clamping box through the air holes to affect the detection result, and on the other hand, bubbles generated in the ultrasonic mixing process can be prevented from entering the external environment through the air holes to cause environmental pollution.

Description

TECHNICAL FIELD

[0001] The embodiment of the present application relates to the technical field of in-vitro medical diagnosis devices, in particular to a nucleic acid detection cartridge. BACKGROUND

[0002] The nucleic acid detection cartridge is usually provided with a plurality of cavities. In the nucleic acid detection process, the nucleic acid sample needs to be transferred to different cavities according to the nucleic acid detection steps, and the nucleic acid sample is processed in the cavities by corresponding reagents. Therefore, at least part of the plurality of cavities needs to be connected to the outside through an air hole, and a positive pressure or a negative pressure is introduced into the cavity through the air hole, so that the fluid in the cartridge flows between the plurality of cavities, thereby realizing the transfer of the sample between the plurality of cavities.

[0003] When processing the nucleic acid sample, the nucleic acid sample and the reagent in the cavity need to be ultrasonically vibrated and mixed. Since the energy of ultrasonic vibration is large, the reagent in the cavity will splash and generate bubbles. These bubbles are easy to enter the channel between the air hole and the cavity and enter the external environment through the air hole, resulting in leakage of the reagent and environmental pollution. SUMMARY

[0004] In view of the above problems, the embodiment of the present application provides a nucleic acid detection cartridge to solve the problem that the bubbles generated by ultrasonic vibration and mixing when processing the nucleic acid sample are easy to enter the external environment through the air hole and cause environmental pollution.

[0005] According to an aspect of the embodiment of the present application, a nucleic acid detection cartridge is provided. The cartridge is provided with a plurality of cavities and at least one air hole. The air hole is in communication with the cavities, and the air hole is used to drive the fluid in the cartridge to flow between the plurality of cavities when a positive pressure or a negative pressure is introduced. A water-absorbing and air-permeable film is arranged between the air hole and the cavity in communication therewith. The water-absorbing and air-permeable film is used to prevent water from entering the inside of the cartridge and eliminate bubbles.

[0006] In an optional manner, a waterproof and air-permeable film is covered on the air hole. The waterproof and air-permeable film is used to prevent leakage of aerosol and vaporized reagent in the inside of the cartridge.

[0007] In an optional manner, the cartridge includes a cartridge body and a cover piece. A plurality of grooves are formed on the side surface of the cartridge body. The cover piece is attached to the side surface of the cartridge body and seals the groove opening of the groove, so that the groove forms a cavity and a channel connecting the cavity and the air hole. The channel has a containing groove with an enlarged bottom area, and the water-absorbing and air-permeable film is filled in the containing groove.

[0008] In an optional mode, the plurality of cavities include an elution cavity, the air holes include a first air hole in communication with the elution cavity, and the water-absorbing and air-permeable membrane includes a first water-absorbing and air-permeable membrane arranged between the first air hole and the elution cavity; a micro valve is arranged in a channel between the first water-absorbing and air-permeable membrane and the elution cavity, and the micro valve is used to reduce the volume of air bubbles.

[0009] In an optional mode, the channel between the first water-absorbing and air-permeable membrane and the elution cavity includes a first channel and a second channel; one end of the first channel is in communication with the first water-absorbing and air-permeable membrane, one end of the second channel is in communication with the elution cavity, a valve groove is arranged on the cartridge body and is in communication with the other end of the first channel and the other end of the second channel, respectively, a blocking piece is arranged in the valve groove, and the blocking piece is used to block air bubbles; the valve groove and the blocking piece jointly form a micro valve.

[0010] In an optional mode, a gap is provided between the blocking piece and the inner wall of the valve groove.

[0011] In an optional mode, the blocking piece includes an elastic pad.

[0012] In an optional mode, the first water-absorbing and air-permeable membrane is in communication with the elution cavity and the first air hole through channels at opposite ends of the first water-absorbing and air-permeable membrane.

[0013] In an optional mode, the first water-absorbing and air-permeable membrane is arranged at an edge or a corner of the cartridge.

[0014] In an optional mode, the plurality of cavities include a sample cavity and a lysis cavity, the sample cavity is in communication with the lysis cavity; the air holes include a second air hole in communication with at least one of the sample cavity and the lysis cavity, and the water-absorbing and air-permeable membrane includes a second water-absorbing and air-permeable membrane arranged between the second air hole and the cavity in communication therewith.

[0015] In an optional mode, the second air hole is in communication with the sample cavity, and the second air hole is used to drive the sample to enter the lysis cavity from the sample cavity when a positive pressure is introduced; the second water-absorbing and air-permeable membrane is arranged between the second air hole and the sample cavity.

[0016] The embodiments of the present application drive the fluid in the cartridge by introducing a positive pressure or a negative pressure into the air hole in communication with the cavity, so that the process of driving the sample liquid to flow in the cartridge is efficient and controllable. In addition, the water-absorbing and air-permeable membrane is arranged between the air hole and the cavity in communication therewith, on the one hand, the water-absorbing and air-permeable membrane can absorb the moisture entering the inside of the cartridge through the air hole, preventing the moisture from entering the inside of the cartridge and affecting the accuracy of the detection structure when the air hole is introduced into the positive pressure; on the other hand, the air bubbles generated in the ultrasonic vibration mixing process will be broken when they encounter the water-absorbing and air-permeable membrane, thereby avoiding the environmental pollution caused by the air bubbles carrying the reagent to the external environment.

[0017] The above description is only a summary of the technical solutions of the present application. In order to enable one skilled in the art to better understand the technical means of the present application and to implement the same according to the contents of the description, and in order to enable the above and other purposes, characteristics and advantages of the present application to be more apparent and easy to understand, the following specific embodiments of the present application are described. BRIEF DESCRIPTION OF DRAWINGS

[0018] Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The accompanying drawings are included to provide a description of the preferred embodiments and are not meant to limit the present application. Moreover, the same reference numerals in the drawings indicate the same or similar elements. In the drawings:

[0019] Figure 1 A perspective view of one side of a nucleic acid detection card provided by an embodiment of the present application is shown.

[0020] Figure 2 A perspective view of the other side of a nucleic acid detection card provided by an embodiment of the present application is shown.

[0021] Figure 3 A perspective view of a nucleic acid detection card provided by an embodiment of the present application is shown.

[0022] Figure 4 A perspective view of a nucleic acid detection card provided by another embodiment of the present application is shown.

[0023] Figure 5 An exploded view of one side of a nucleic acid detection card provided by an embodiment of the present application is shown.

[0024] Figure 6 An exploded view of the other side of a nucleic acid detection card provided by an embodiment of the present application is shown.

[0025] Figure 7 An enlarged view of part A in FIG. Figure 6

[0026] Reference numerals in the detailed description are as follows:

[0027] 100, card box;

[0028] 10, cavity; 20, air hole; 30, water-absorbing and air-permeable film; 40, waterproof and air-permeable film; 50, card box body; 60, cover; 70, channel; 80, sample inlet;

[0029] 11, sample cavity; 12, magnetic bead cavity; 13, lysis cavity; 14, elution cavity; 15, reaction cavity;

[0030] 21, first air hole; 22, second air hole;

[0031] ​31, first water-absorbing and air-permeable film; 32, second water-absorbing and air-permeable film;

[0032] 71, accommodating groove; 72, micro valve; 73, first passage; 74, second passage; 75, third passage;

[0033] 721, valve groove; 722, blocking piece; 723, micro hole. DETAILED DESCRIPTION

[0034] The embodiments of the technical solutions of the present application will be described in detail below with reference to the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solutions of the present application, and therefore only serve as examples, and cannot limit the protection scope of the present application.

[0035] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the present application; the terms "include" and "have" and any variations thereof in the specification and claims of the present application and the above description of drawings are intended to cover non-exclusive inclusion.

[0036] In the description of the embodiments of the present application, the technical terms "first", "second", etc. are only used to distinguish different objects, and cannot be understood as indicating or implying relative importance or implicitly indicating the number, specific order or primary and secondary relationship of the indicated technical features. In the description of the embodiments of the present application, the meaning of "multiple" is more than two, unless otherwise explicitly and specifically limited.

[0037] Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the present application. The appearance of this phrase in various places in the specification does not necessarily all refer to the same embodiment, nor is it necessarily mutually exclusive or alternative to other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0038] In the description of the embodiments of the present application, the term "and / or" is only a description of the association relationship of the associated objects, which means that there can be three relationships, for example, A and / or B, which can represent the three cases of existence of A, existence of A and B, and existence of B. In addition, the character " / " in this paper generally represents that the front and rear associated objects are a "or" relationship.

[0039] In the description of the embodiments of the present application, the term "multiple" refers to more than two (including two), and similarly, "multiple groups" refers to more than two groups (including two groups), and "multiple pieces" refers to more than two pieces (including two pieces).

[0040] In the description of the embodiments of the present application, the orientations or positional relationships indicated by the technical terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the embodiments of the present application and simplifying the description, and are not intended to indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the embodiments of the present application.

[0041] In the description of the embodiments of the present application, unless otherwise explicitly specified and limited, the technical terms "mounting", "connecting", "connecting", "fixing" and the like should be understood broadly, for example, can be fixed connection, can also be detachable connection, or integral; can be mechanical connection, can also be electrical connection; can be directly connected, can also be indirectly connected through an intermediate medium, can be the internal communication of two elements or the interaction relationship between two elements. For those skilled in the art, the specific meanings of the above terms in the embodiments of the present application can be understood according to the specific circumstances.

[0042] The nucleic acid detection technology generally includes steps of lysis, binding, washing, elution, and subsequent PCR (polymerase chain reaction, also known as in vitro DNA amplification technology) and optical detection. Among them, the steps of lysis, binding, washing, elution, etc. are used to process the sample to extract the nucleic acid carried by the bacteria and / or viruses in the sample, and the steps of PCR and optical detection are used to detect the extracted nucleic acid. In order to ensure the accuracy of nucleic acid detection, sample processing and nucleic acid detection are usually carried out separately, that is, a plurality of cavities are usually provided on the cartridge, and these cavities are respectively used for sample processing and nucleic acid detection. Moreover, in order to facilitate the transfer of the sample, an air hole is also provided on the cartridge to communicate the cavity with the outside, so as to introduce positive pressure or negative pressure into the cavity through the air hole, thereby driving the sample to flow between the cavities.

[0043] In addition, when processing the sample, the sample and reagents (such as lysis reagent, binding reagent, washing reagent, elution reagent, etc.) in the cavity can also be ultrasonically vibrated and mixed to fully process the sample and extract nucleic acid with higher purity. However, since the energy of ultrasonic vibration is large, bubbles will be generated in the reagents in the cavity when processing the sample, these bubbles will reach the air hole through the channel between the air hole and the cavity, and enter the external environment through the air hole, thereby causing the reagents carried by the bubbles to leak and pollute the environment.

[0044] Based on this, the nucleic acid detection card kit provided in the present application is provided with a water-absorbing and air-permeable film between the air hole and the cavity, so that the bubbles need to pass through the water-absorbing and air-permeable film before reaching the air hole. However, the bubbles will break when they hit the water-absorbing and air-permeable film, at which time the gas carried by the bubbles can pass through the water-absorbing and air-permeable film, and the liquid reagent carried by the bubbles will be absorbed by the water-absorbing and air-permeable film, so that the leakage of the reagent and the resulting environmental pollution can be effectively avoided. In addition, when the air hole is introduced into the positive pressure, the water-absorbing and air-permeable film can also absorb the moisture entering the inside of the card kit from the air hole, thereby avoiding the problem of moisture entering the inside of the card kit and affecting the accuracy of the detection results.

[0045] According to an aspect of an embodiment of the present application, a nucleic acid detection card kit is provided, which is specifically described with reference to Figures 1 to 3 , Figure 1 and Figure 2 show the perspective structures of the two sides of the card kit, Figure 3 show the perspective structure of the card kit from the front view, it should be noted that, in the specific embodiment shown in Figure 3 , each channel and cavity is formed by a groove opened on the card kit, and the groove is covered with a film or baffle at the slot opening to close the cavity and flow channel, the black solid line part in the figure represents the groove structure that can be seen from the front, and the gray dashed line part represents the groove structure that cannot be seen from the back, the elongated part in the figure is the channel, and the channel at the position where the solid line and the dashed line meet is connected by a through hole penetrating through the card kit. It can be understood that the card kit provided in the embodiment is only an example, and in other embodiments, all flow channels and cavities can also be arranged on one side of the card kit, or an integrally formed card kit can also be used, and the cavities and flow channels can be formed in the card kit during the injection molding process.

[0046] As shown in Figure 3 , the card kit 100 is provided with a plurality of cavities 10 and at least one air hole 20, the air hole 20 is in communication with the cavities 10, and the air hole 20 is used to drive the fluid in the card kit 100 to flow between the plurality of cavities 10 when the positive pressure or negative pressure is introduced. The water-absorbing and air-permeable film 30 is arranged between the air hole 20 and the cavity 10 in communication therewith, and the water-absorbing and air-permeable film 30 is used to prevent moisture from entering the inside of the card kit 100 and eliminate bubbles.

[0047] The cavities 10 are various functional spaces on the cartridge 100, such as a sample cavity 11 for injecting a sample liquid, a magnetic bead cavity 12 for storing magnetic beads (i.e. lyophilized beads), a lysis cavity 13 and an elution cavity 14 for processing a sample, and a reaction cavity 15 for detecting nucleic acid, etc. In the process of nucleic acid detection, the sample needs to be transferred to the corresponding cavity 10 according to the steps of nucleic acid detection, for example, the sample needs to be transferred from the sample cavity 11 to the lysis cavity 13 or the elution cavity 14 when processing the sample, and the sample needs to pass through the magnetic bead cavity 12 during the transfer process so that the sample is adsorbed on the magnetic beads; when detecting nucleic acid, the sample needs to be transferred from the lysis cavity 13 or the elution cavity 14 to the reaction cavity 15. In some embodiments, only one sample processing cavity can be provided on the cartridge 100, and the functions of the lysis cavity 13 and the elution cavity 14 can be realized through the sample processing cavity.

[0048] In order to facilitate the transfer of the sample, at least one air hole 20 is provided on the cartridge 100 and communicates with the cavities 10, so as to provide power for the fluid (such as the sample liquid) inside the cartridge 100 through the air hole 20, thereby driving the fluid to flow between the cavities 10. Specifically, as an example, as shown in Figure 3 the air hole 20 includes a first air hole 21 communicating with the elution cavity 14 on the cartridge 100, when a positive pressure is introduced into the first air hole 21, the pressure in the elution cavity 14 will increase, thereby driving the fluid in the elution cavity 14 to exit the elution cavity 14 and enter other cavities 10 inside, so as to maintain the pressure balance between the elution cavity 14 and other cavities 10 and ensure the stability of the cartridge 100. Similarly, when a negative pressure is introduced into the first air hole 21, the pressure in the elution cavity 14 will decrease, thereby driving the fluid in other cavities 10 to enter the elution cavity 14. Specifically, when performing nucleic acid operation, the cartridge 100 can be used with a detection device, the detection device is provided with a gas pipeline for connecting with the air hole 20, gas is blown into the air hole 20 by the gas pipeline to realize the access of positive pressure of the air hole 20, and gas is extracted from the air hole 20 by the gas pipeline to realize the access of negative pressure of the air hole 20.

[0049] The water-absorbing and air-permeable film 30 has the performance of absorbing liquid and allowing gas to pass through. Specifically, when the gas hole 20 is introduced with positive pressure, the moisture in the gas entering the inside of the cartridge 100 is absorbed by the water-absorbing and air-permeable film 30 when passing through the water-absorbing and air-permeable film 30, and the gas normally enters the cavity 10 in communication with the gas hole 20. Of course, when the magnetic beads are dried, the water vapor evaporated during drying is also absorbed by the water-absorbing and air-permeable film 30 when passing through the water-absorbing and air-permeable film 30. In addition, when the sample and reagent in the cavity 10 are ultrasonically vibrated and mixed, not only will the reagent splash and bubbles be generated, but the pressure in the cavity 10 will also increase. At this time, the pressure at the gas hole 20 on the cartridge 100 will be lower than the pressure in the cavity 10, thereby driving the bubbles to move towards the gas hole 20. When the bubbles reach the water-absorbing and air-permeable film 30, the liquid on the surface of the bubbles will be absorbed by the water-absorbing and air-permeable film 30, thereby causing the bubbles to burst, that is, the reagent carried by the bubbles will be absorbed by the water-absorbing and air-permeable film 30, and the gas will continue to move towards the gas hole 20.

[0050] The position of the water-absorbing and air-permeable film 30 is determined by the position of the gas hole 20, that is, the gas hole 20 is in communication with which cavity 10, the water-absorbing and air-permeable film 30 is arranged between the cavity 10 and the gas hole 20 to avoid the liquid (i.e. moisture or reagent) flowing between the outside environment and the inside of the cartridge 100 through the gas hole 20. Specifically, in the embodiment shown in FIG. 1, the water-absorbing and air-permeable film 30 is arranged between the gas hole 20 and the cavity 10 in communication with the gas hole 20, that is, the water-absorbing and air-permeable film 30 is arranged between the gas hole 20 and the sample cavity 11. Figure 3 In the specific embodiment shown, the gas hole 20 includes a first gas hole 21 in communication with the elution cavity 14 and a second gas hole 22 in communication with the lysis cavity 13, and therefore the first water-absorbing and air-permeable film 31 is arranged between the first gas hole 21 and the elution cavity 14, and the second water-absorbing and air-permeable film 32 is arranged between the second gas hole 22 and the lysis cavity 13. Figure 4 A perspective view of the front view of the nucleic acid detection cartridge according to another embodiment of the present application is shown, in which the gas hole 20 also includes a first gas hole 21 in communication with the elution cavity 14 and a second gas hole 22 in communication with the sample cavity 11, wherein the position of the first water-absorbing and air-permeable film 31 is the same as that of the embodiment shown in FIG. 1, and the second water-absorbing and air-permeable film 32 is arranged between the second gas hole 22 and the sample cavity 11. Figure 3 The position of the water-absorbing and air-permeable film 30 is determined by the position of the gas hole 20, that is, the gas hole 20 is in communication with which cavity 10, the water-absorbing and air-permeable film 30 is arranged between the cavity 10 and the gas hole 20 to avoid the liquid (i.e. moisture or reagent) flowing between the outside environment and the inside of the cartridge 100 through the gas hole 20. Specifically, in the embodiment shown in FIG. 1, the water-absorbing and air-permeable film 30 is arranged between the gas hole 20 and the cavity 10 in communication with the gas hole 20, that is, the water-absorbing and air-permeable film 30 is arranged between the gas hole 20 and the sample cavity 11.

[0051] In the above embodiment, the gas hole 20 is arranged on the cartridge 100 to communicate with the cavity 10, and the driving of the fluid in the cartridge 100 is realized by connecting the positive pressure or negative pressure to the gas hole 20, so that the process of driving the sample liquid to flow in the cartridge 100 is efficient and controllable. In addition, the water-absorbing and air-permeable film 30 is arranged between the gas hole 20 and the cavity 10 communicating therewith. On the one hand, the water-absorbing and air-permeable film 30 can absorb the moisture entering the inside of the cartridge 100 through the gas hole 20, preventing the moisture from entering the inside of the cartridge 100 and affecting the accuracy of the detection structure when the positive pressure is connected to the gas hole 20. On the other hand, the bubbles generated in the ultrasonic vibration mixing process will be broken when they encounter the water-absorbing and air-permeable film 30, thereby avoiding the environmental pollution caused by the bubbles carrying the reagent to the external environment.

[0052] In order to sufficiently reduce the pollution to the detection environment, the application further provides an embodiment as shown in Figure 3 and Figure 5 , Figure 5 which shows the explosion structure of the cartridge from one side view. The gas hole 20 is covered with a waterproof and air-permeable film 40, which is used to prevent the leakage of aerosol and vaporized reagent in the cartridge 100. Specifically, the waterproof and air-permeable film 40 can be covered at the opening of the gas hole 20 as shown in Figure 3 and Figure 5 , or can be covered in the channel between the gas hole 20 and the cavity 10. Such a structure can use the waterproof and air-permeable film 40 to close the gas hole 20 that communicates the internal space of the cartridge 100 with the external space, thereby effectively preventing the internal aerosol from polluting the detection environment.

[0053] Considering that the way of integrally forming the microfluidic channel in the cartridge 100 has the problems of complex process and high cost, the application provides a manufacturing method that is convenient for batch production of the cartridge 100 and can effectively reduce the cost. Please refer to Figure 5 and Figure 6 , Figure 6 which shows the explosion structure of the cartridge from another side view. The cartridge 100 includes a cartridge body 50 and a cover piece 60. The cartridge body 50 has a plurality of grooves on the side surface, and the cover piece 60 is attached to the side surface of the cartridge body 50 and seals the groove opening of the groove, so that the groove forms the cavity 10 and the channel 70 that communicates the cavity 10 and the gas hole 20.

[0054] In the specific generation process, in order to ensure the structural strength, the card box body 50 can be made of hard material, and in order to ensure the sealing performance, the cover piece 60 can be made of flexible material, and the two can be assembled and fixed by bonding, hot melting and the like. Compared with the way of integrally forming the channel 70 and the cavity 10 inside, the way of opening a groove in the card box body 50 and sealing the groove by the cover piece 60 provided by the embodiment is more convenient for the generation and manufacturing of the card box 100, which is beneficial to improve the generation efficiency and reduce the production cost.

[0055] In addition, it should be noted that the air permeable water absorption film currently has a relatively serious decline in air permeability after absorbing a large amount of liquid. Considering that the liquid inside the cavity 10 will splash during ultrasonic vibration and mixing of the nucleic acid sample and reagent in the cavity, based on this, as shown in Figure 3 and Figure 4 , a large amount of liquid may pass through the channel 70 to the air permeable water absorption film 30 and be absorbed by the air permeable water absorption film 30, causing the air permeability of the air permeable water absorption film 30 to decrease, and when the air pressure is introduced through the air hole 20, the passing property of the gas at the air permeable water absorption film 30 decreases, and the driving ability of the internal liquid decreases, which may even cause the sample to not flow normally between the cavity 10 and the channel 70 in severe cases.

[0056] Therefore, as shown in Figures 3 to 5 , the channel 70 has a containing groove 71 with an enlarged bottom area, and the air permeable water absorption film 30 is filled in the containing groove 71. Specifically, as shown in the figure, the air permeable water absorption film 30 is arranged in a flat state in the containing groove 71, that is, the area of the air permeable water absorption film 30 is equal to the bottom area of the containing groove 71, so that the air permeable water absorption film 30 has a large area to prevent clogging when a large amount of liquid is absorbed.

[0057] The size of the bubbles generated by ultrasonic vibration and mixing is different. In the same size channel, the air proportion in the large bubble is greater than that in the small bubble, so the weight of the large bubble is also lighter than that of the small bubble. Under the action of the same pressure, the moving speed of the large bubble in the channel 70 is faster than that of the small bubble, which may cause the large bubble to pass through the surface of the air permeable water absorption film 30 and then pass through the air hole to the external environment, causing environmental pollution, or causing the waterproof and air permeable film 40 covering the air hole 20 to be wetted by the liquid carried by the bubble and fail, and then the gas cannot pass through the waterproof and air permeable film 40 to affect the transfer of the fluid.

[0058] Therefore, in order to prevent the large bubble from passing through the surface of the air permeable water absorption film 30, the present application further provides an embodiment, as shown in Figures 4 to 6As shown, the multiple cavities 10 include an elution chamber 14, an air pore 20 including a first air pore 21 communicating with the elution chamber 14, and a water-absorbing breathable membrane 30 including a first water-absorbing breathable membrane 31 disposed between the first air pore 21 and the elution chamber 14. A micro valve 72 is disposed in the channel 70 between the first water-absorbing breathable membrane 31 and the elution chamber 14. The micro valve 72 is used to reduce the volume of air bubbles. The function of the micro valve 72 is to eliminate large air bubbles or convert large air bubbles into small air bubbles when the elution chamber 14 is subjected to ultrasonic vibration mixing treatment, so that they are quickly absorbed when they reach the first water-absorbing breathable membrane 31, preventing large air bubbles from passing through the first water-absorbing breathable membrane 31 and reaching the first air pore 21, thereby preventing environmental pollution or failure of the waterproof breathable membrane 40 covering the first air pore 21.

[0059] Specifically, such as Figures 5 to 7 As shown, Figure 7 It shows Figure 6 The enlarged schematic diagram at point A shows that the channel 70 between the first absorbent and breathable membrane 31 and the elution chamber 14 includes a first channel 73 and a second channel 74. One end of the first channel 73 is connected to the first absorbent and breathable membrane 31, and one end of the second channel 74 is connected to the elution chamber 14. A valve groove 721 is provided on the cartridge body 50, which is connected to the other end of the first channel 73 and the other end of the second channel 74. A blocking member 722 is placed inside the valve groove 721 to block air bubbles. The valve groove 721 and the blocking member 722 together form a micro-valve 72.

[0060] During the ultrasonic vibration mixing process in the elution chamber 14, bubbles generated in the elution chamber 14 may splash into the second channel 74 and move along the second channel 74 to the micro valve 72 under pressure. When the bubbles enter the valve groove 721 from the second channel 74, they are blocked by the blocking member 722 and collide with the blocking member 722 and break. Specifically, the blocking member 722 may include an elastic pad.

[0061] Furthermore, there is a gap between the blocking member 722 and the inner wall of the valve groove 721. When a bubble reaches the micro valve 72, the smaller bubble can enter the first channel 73 through the gap between the blocking member 722 and the inner wall of the valve groove 721, so as to reach the first absorbent breathable membrane 31 through the first channel 73 and be absorbed by the first absorbent breathable membrane 31.

[0062] The large bubbles entering the valve groove 721 from the second channel 74 can be broken by colliding with the blocking piece 722 or broken or converted into small bubbles when passing through the gap between the inner wall of the valve groove 721 and the blocking piece 722. That is, the large bubbles reaching the micro valve 72 can be eliminated or converted into small bubbles by colliding with the blocking piece 722 and entering the small gap from the large section, and then quickly absorbed when reaching the first water-absorbing and air-permeable membrane 31. Specifically, as shown in Figure 7 When the first channel 73 and the second channel 74 are arranged on the two sides of the cartridge 100 respectively, the second channel 74 can be in communication with the side wall of the valve groove 721, and the first channel 73 is in communication with the micro hole 723 arranged through the bottom of the valve groove 721. After the bubbles enter the micro valve 72 from the first channel 73, they first pass through the gap between the side wall of the valve groove 721 and the blocking piece 722 to reach the bottom of the valve groove 721, then pass through the gap between the inner wall of the bottom of the valve groove 721 and the blocking piece 722 to reach the micro hole 723, and finally pass through the micro hole 723 into the first channel 73. In this process, the large bubbles are broken by the extrusion of the inner wall of the valve groove 721 and the blocking piece 722.

[0063] Of course, the micro valve 72 can also be arranged as a flow blocking valve. Before the sample in the elution chamber 14 is ultrasonically mixed, the micro valve 72 can be closed in advance to ensure that the liquid does not splash to the first water-absorbing and air-permeable membrane 31, thereby avoiding the influence of the first water-absorbing and air-permeable membrane 31 caused by absorbing too much liquid, and ensuring the driving ability of the sample when the pressure is introduced into the first air hole 21.

[0064] In the above embodiment, the micro valve 72 is arranged between the elution chamber 14 and the first water-absorbing and air-permeable membrane 31 to eliminate or convert the large bubbles generated during the ultrasonic mixing process into small bubbles through the micro valve 72, thereby avoiding the large bubbles passing through the surface of the first water-absorbing and air-permeable membrane 31 to cause environmental pollution or cause the waterproof and air-permeable membrane 40 covering the first air hole 21 to fail.

[0065] The water-absorbing and air-permeable membrane 30 mainly contacts the bubbles and absorbs the liquid on the surface of the bubbles to break the bubbles and achieve the purpose of eliminating the bubbles. However, under the action of pressure, the bubbles will continue to move along the surface of the water-absorbing and air-permeable membrane 30 after reaching the water-absorbing and air-permeable membrane 30. If the two positions of the water-absorbing and air-permeable membrane 30 communicating with the chamber 10 and the air hole 20 are relatively close, the bubbles reaching the water-absorbing and air-permeable membrane 30 from the chamber 10 only need to move a small distance on the surface of the water-absorbing and air-permeable membrane 30 to enter the channel 70 between the water-absorbing and air-permeable membrane 30 and the air hole 20, which may cause the bubbles to be in contact with the water-absorbing and air-permeable membrane 30 for too short a time to be absorbed by the water-absorbing and air-permeable membrane 30.

[0066] Therefore, in order to make the bubbles and the water-absorbing and air-permeable film 30 fully contact, the present application further provides an embodiment as shown in Figure 3 and Figure 4 As shown in the figure, the right end of the first water-absorbing and air-permeable film 31 is communicated with the elution cavity 14 through the channel 70, and the first air hole 21 is communicated with the position close to the left end of the first water-absorbing and air-permeable film 31 through the channel 70, so that the bubbles reach the first water-absorbing and air-permeable film 31 from the elution cavity 14 and then pass through the first water-absorbing and air-permeable film 31 from right to left before entering the channel 70 between the first water-absorbing and air-permeable film 31 and the first air hole 21, so as to ensure that the bubbles fully contact and are absorbed by the first water-absorbing and air-permeable film 31.

[0067] Of course, the upper and lower ends of the first water-absorbing and air-permeable film 31 can also be communicated with the elution cavity 14 and the first air hole 21 through the channel 70 respectively, so that the bubbles pass through the first water-absorbing and air-permeable film 31 from bottom to top or from top to bottom after reaching the first water-absorbing and air-permeable film 31. In addition, in order to make the bubbles move farther on the first water-absorbing and air-permeable film 31, the elution cavity 14 and the first air hole 21 can also be communicated with the opposite two end corners of the first water-absorbing and air-permeable film 31 respectively, for example, in Figure 4 the top end of the right side of the first water-absorbing and air-permeable film 31 is communicated with the elution cavity 14, and the bottom end of the left side of the first water-absorbing and air-permeable film 31 is communicated with the first air hole 21.

[0068] The above embodiment, by communicating the elution cavity 14 and the first air hole 21 with the opposite two ends of the first water-absorbing and air-permeable film 31 respectively, makes the bubbles fully contact with the first water-absorbing and air-permeable film 31 after reaching the first water-absorbing and air-permeable film 31 from the elution cavity 14, effectively utilizes the performance of the first water-absorbing and air-permeable film 31 to absorb liquid to eliminate the bubbles, and avoids the bubbles from passing through the first water-absorbing and air-permeable film 31 to reach the first air hole 21, which causes environmental pollution or leads to the failure of the waterproof and air-permeable film 40 covering the first air hole 21.

[0069] It should be particularly pointed out that when the water-absorbing and air-permeable film 30 further includes other water-absorbing and air-permeable films in addition to the first water-absorbing and air-permeable film 31, the other water-absorbing and air-permeable films can also be communicated with the cavity 10 and the air hole 20 through the channel 70 at their opposite two ends respectively, so that the bubbles fully contact with the other water-absorbing and air-permeable films after reaching the other water-absorbing and air-permeable films from the cavity 10.

[0070] Considering that the middle region of the cartridge 100 needs to integrate more cavities 10 and channels 70, in order to make the first water-absorbing and air-permeable film 31 have a larger area, the present application further provides an embodiment as shown in Figure 4 and Figure 5As shown, the first water-absorbing and air-permeable film 31 is arranged at the edge or corner of the cartridge 100, so that the area of the first water-absorbing and air-permeable film 31 can be as large as possible. Figure 3 And Figure 4 As can be seen, Figure 4 Compared with the embodiment shown in Figure 3 In the embodiment shown, the first water-absorbing and air-permeable film 31 is arranged at the edge or corner of the cartridge 100, so that the area of the first water-absorbing and air-permeable film 31 can be as large as possible.

[0071] Of course, if the water-absorbing and air-permeable film 30 further includes other water-absorbing and air-permeable films in addition to the first water-absorbing and air-permeable film 31, the other water-absorbing and air-permeable films can also be arranged at the edge or corner of the cartridge 100, so that the area of the other water-absorbing and air-permeable films can be as large as possible, thereby preventing clogging in the case of absorbing a large amount of liquid.

[0072] In addition, in order to prevent the reagent splashed into the channel 70 from reaching the first air hole 21, the present application further provides an embodiment, as shown in Figure 4 The first air hole 21 is arranged at the middle position of the cartridge 100, and the first water-absorbing and air-permeable film 31 is connected to the first air hole 21 through the curved channel 70 at the position away from the first air hole 21, so that the channel 70 between the first water-absorbing and air-permeable film 31 and the first air hole 21 can be as long as possible.

[0073] Specifically, as shown in Figure 4 The reagent splashed into the channel 70 needs to pass through the first water-absorbing and air-permeable film 31 and then pass through the channel 70 (i.e. the third channel 75) between the first water-absorbing and air-permeable film 31 and the first air hole 21 before reaching the first air hole 21. Since the third channel 75 extends in a curved manner and the first water-absorbing and air-permeable film 31 is arranged at the edge or corner, while the first air hole 21 is arranged at the middle position, the third channel 75 can be as long as possible, thereby preventing the reagent and air bubbles from reaching the first air hole 21 to a certain extent, preventing the reagent and air bubbles from reaching the first air hole 21 through the channel 70 to cause environmental pollution or the failure of the waterproof and air-permeable film 40 covering the first air hole 21.

[0074] In order to facilitate the control of the movement of fluid between the plurality of cavities, as shown in Figure 3 And Figure 4 The plurality of cavities 10 includes a sample cavity 11 and a lysis cavity 13, the sample cavity 11 is in communication with the lysis cavity 13, the air hole 20 includes a second air hole 22 in communication with at least one of the sample cavity 11 and the lysis cavity 13, and the water-absorbing and air-permeable film 30 includes a second water-absorbing and air-permeable film 32 arranged between the second air hole 22 and the cavity 10 in communication therewith.

[0075] As shown in Figure 3 , the second air hole 22 can be in communication with the lysis cavity 13, and the second water-absorbing and air-permeable film 32 is arranged between the second air hole 22 and the lysis cavity 13. When the second air hole 22 is connected to negative pressure, the pressure in the lysis cavity 13 decreases, and then the sample in the sample cavity 11 is sucked into the lysis cavity 13. When the second air hole 22 is connected to positive pressure, the pressure in the lysis cavity 13 increases, and then the fluid in the lysis cavity 13 is pumped into the rear cavity 10.

[0076] In order to ensure the convenience of sample injection, the sample inlet 80 is generally arranged to be relatively large, and the volume of the sample cavity 11 is also arranged to be relatively large. It is found through practice that the sample cavity 11 with a large volume will cause more sample liquid to hang on the inner wall of the sample cavity 11 to form small liquid beads. Based on this, when the second air hole 22 is in communication with the lysis cavity 13 as shown in Figure 3 , in the process of introducing negative pressure into the second air hole 22 to suck the sample liquid from the sample cavity 11 into the lysis cavity 13, due to the characteristics of negative pressure driving, the small liquid beads hanging on the inner wall of the sample cavity 11 cannot be effectively sucked into the lysis cavity 13, so that this part of the small liquid beads cannot participate in the subsequent sample processing process, thereby affecting the processing capacity of the cartridge 100 to the sample.

[0077] In view of the above problems, in addition to the specific structure as shown in Figure 3 , in some other embodiments, such as the two-face perspective exploded structure and perspective structure as shown in Figures 4 to 6 , the second air hole 22 is in communication with the sample cavity 11. The second air hole 22 is used to drive the sample from the sample cavity 11 into the lysis cavity 13 when positive pressure is introduced. The second water-absorbing and air-permeable film 32 is arranged between the second air hole 22 and the sample cavity 11.

[0078] When the second air hole 22 introduces positive pressure, the pressure in the sample cavity 11 increases, and then the sample in the sample cavity 11 is pumped into the lysis cavity 13. In this process, the direction of the gas flowing from the second air hole 22 to the sample cavity 11 is shown by the dashed arrow in the channel 70 between the second air hole 22 and the sample cavity 11 as shown in Figure 4 . By comparing Figure 3 and Figure 4 , it can be seen that the specific embodiments shown in the two figures are basically the same in structure except that the cavity 10 in communication with the second air hole 22 is different.

[0079] After the second air hole 22 is communicated with the sample cavity 11, and the sample is pushed to flow from the sample cavity 11 to the lysis cavity 13 by introducing positive pressure in the second air hole 22, based on the characteristics of the positive pressure driving, the gas in the sample cavity 11 can push most of the small liquid beads hanging on the inner wall of the sample cavity 11 along the inner wall and the channel 70 into the lysis cavity 13, so as to ensure that more samples participate in subsequent processing and detection, thereby improving the sample processing capacity and improving the detection effect.

[0080] In addition, when the sample and the reagent in the lysis cavity 13 are mixed by ultrasonic vibration, the bubbles or splashed reagents generated in the lysis cavity 13 need to enter the sample cavity 11 through the channel 70 between the lysis cavity 13 and the sample cavity 11, and then reach the second water-absorbing and air-permeable film 32 through the channel 70 between the sample cavity 11 and the second water-absorbing and air-permeable film 32. However, the reagent entering the sample cavity 11 will deposit at the bottom of the sample cavity 11 under the action of gravity, and the bubbles may be broken by colliding with the wall of the channel 70 during the movement in the channel 70 between the lysis cavity 13 and the sample cavity 11. Therefore, such a structure design can effectively avoid the decrease of the air permeability of the second water-absorbing and air-permeable film 32 due to the absorption of too much liquid, and the liquid in the channel 70 between the lysis cavity 13 and the sample cavity 11 and the sample cavity 11 can also be pumped into the lysis cavity 13 by introducing positive pressure into the second air hole 22.

[0081] Of course, in some other embodiments, at least two second air holes 22 can also be arranged on the cartridge 100, at least one of which is communicated with the sample cavity 11, and at least one of which is communicated with the lysis cavity 13. When the sample is driven, positive pressure can be introduced into the second air hole 22 communicated with the sample cavity 11, and negative pressure can be introduced into the second air hole 22 communicated with the lysis cavity 13.

[0082] According to another aspect of the embodiments of the present application, a nucleic acid detection system is also provided, which comprises a detection device (not shown in the figure) and the nucleic acid detection cartridge 100 in any of the above embodiments. The detection device is used to fix and operate the nucleic acid detection cartridge to perform nucleic acid detection.

[0083] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, rather than limit them. Although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that the technical solutions recorded in the foregoing embodiments can be modified, or some or all of the technical features can be replaced equivalently. Such modifications or replacements do not change the essence of the corresponding technical solutions, which should be covered in the scope of the claims and the specification of the present application. In particular, the technical features mentioned in each embodiment can be combined in any manner as long as there is no structural conflict. The present application is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A nucleic acid testing cartridge, characterized by, The cartridge is provided with a plurality of cavities and at least one air hole communicating with the cavities, and the air hole is used to drive the fluid in the cartridge to flow between the cavities when a positive pressure or a negative pressure is introduced. A water-absorbing and air-permeable film is arranged between the air hole and the cavities communicating therewith, and the water-absorbing and air-permeable film is used to prevent moisture from entering the interior of the cartridge and eliminate air bubbles.

2. The nucleic acid testing cartridge of claim 1, wherein, A waterproof and air-permeable film is arranged on the air hole, and the waterproof and air-permeable film is used to prevent aerosol and reagent after vaporization in the interior of the cartridge from leaking.

3. The nucleic acid testing cartridge of claim 1 or 2, wherein, The cartridge comprises a cartridge body and a cover, a plurality of grooves are formed in the side surface of the cartridge body, and the cover is attached to the side surface of the cartridge body and seals the groove openings of the grooves, so that the grooves form the cavities and channels communicating the cavities and the air hole. The channel has a containing groove with an enlarged bottom area, and the water-absorbing and air-permeable film is filled in the containing groove.

4. The nucleic acid testing cartridge of claim 3, wherein, The plurality of cavities include an elution cavity, the air hole includes a first air hole communicating with the elution cavity, and the water-absorbing and air-permeable film includes a first water-absorbing and air-permeable film arranged between the first air hole and the elution cavity. A micro valve is arranged in the channel between the first water-absorbing and air-permeable film and the elution cavity, and the micro valve is used to reduce the volume of air bubbles.

5. The nucleic acid testing cartridge of claim 4, wherein, The channel between the first water-absorbing and air-permeable film and the elution cavity includes a first channel and a second channel. One end of the first channel communicates with the first water-absorbing and air-permeable film, one end of the second channel communicates with the elution cavity, a valve groove is arranged on the cartridge body and communicates with the other end of the first channel and the other end of the second channel, respectively, a blocking piece is arranged in the valve groove, and the blocking piece is used to block air bubbles. The valve groove and the blocking piece jointly form the micro valve.

6. The nucleic acid testing cartridge of claim 5, wherein, The blocking piece has a gap with the inner wall of the valve groove.

7. The nucleic acid testing cartridge of claim 5, wherein, The blocking piece includes an elastic pad.

8. The nucleic acid testing cartridge of claim 4, wherein, The first water-absorbing and air-permeable film communicates with the elution cavity and the first air hole through channels at opposite ends thereof, respectively.

9. The nucleic acid testing cartridge of claim 4, wherein, The first water-absorbing and air-permeable film is arranged at an edge or a corner position of the cartridge.

10. The nucleic acid testing cartridge of claim 1 or 2, wherein, The plurality of cavities include a sample cavity and a lysis cavity, and the sample cavity communicates with the lysis cavity. The air hole includes a second air hole communicating with at least one of the sample cavity and the lysis cavity, and the water-absorbing and air-permeable film includes a second water-absorbing and air-permeable film arranged between the second air hole and the cavities communicating therewith.

11. The nucleic acid testing cartridge of claim 10, wherein, The second air hole communicates with the sample cavity, and the second air hole is used to drive the sample from the sample cavity into the lysis cavity when a positive pressure is introduced. The second water-absorbing and air-permeable film is arranged between the second air hole and the sample cavity.

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