Reagent and method for improving germination rate of soybean seeds
By using a seed germination rate reagent composed of L-glutamic acid and 6-benzyladenine, along with double disinfection treatment, the problems of low soybean seed germination rate and contamination were solved, achieving efficient and low-cost seed germination and healthy growth.
Patent Information
- Application Number
- CN202511603895.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-04
- Publication Date
- 2026-01-23
AI Technical Summary
Existing technologies for improving soybean seed germination rates suffer from problems such as poor safety, high cost, slow seed emergence, weak germination vigor, and susceptibility to contamination. Furthermore, gibberellin is costly to use and requires high concentrations.
A low-cost seed germination rate reagent was prepared by combining L-glutamic acid and 6-benzyladenine with double sterilization treatment using ethanol and sodium hypochlorite solution, for use in the soaking treatment of soybean seeds.
It significantly improves the germination rate of soybean seeds, ensures good plant growth without deformities within one month after germination, solves the problems of contamination and low germination rate during seed germination, and reduces costs.
Smart Images
Figure CN121369404A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of seed germination technology, specifically to a reagent and method for improving the germination rate of soybean seeds. Background Technology
[0002] Soybeans ( Glycine max Soybean is one of my country's important food crops, rich in plant protein and oil, both of which have high economic value. However, during storage, soybean seeds are affected by temperature, humidity, oxygen, and time, leading to natural aging, fat oxidation, reduced protein activity, and cell structure damage, resulting in a gradual decline in seed viability and germination rate. Seed germination rate directly affects field emergence rate, laying the foundation for subsequent plant growth and yield formation. Low germination rates can lead to missing seedlings and gaps in rows, impacting overall soybean yield. In scientific research and production practice, improving the germination rate of valuable soybean seeds is crucial for ensuring the smooth progress of subsequent work and plays a pivotal role in the development of the soybean industry.
[0003] Currently, there is relatively little research on soybean seed germination technology in China. Existing methods for treating seeds to improve germination rates mainly employ physical methods such as electromagnetic fields and low-energy ion irradiation, but these methods have poor safety and high costs. Another method is hot water soaking, but this method results in slow seed emergence, weak germination, and is prone to large-scale bacterial and fungal contamination when seed quality is poor.
[0004] Plant hormones play a crucial regulatory role in seed dormancy, germination, and seedling growth and development, exhibiting the significant advantage of high efficiency in minute quantities. Among numerous plant hormones, gibberellin (GA3), a commonly used exogenous hormone, effectively breaks seed dormancy and is a core factor in promoting seed germination. However, gibberellin is relatively expensive and requires high concentrations for application. Therefore, developing a method to effectively improve soybean seed germination rate is of great practical significance for advancing soybean seed breeding and solving practical problems encountered in the breeding process. Summary of the Invention
[0005] This invention provides a reagent and a method for improving the germination rate of soybean seeds. The reagent provided by this invention is inexpensive and readily available, and the method for improving the germination rate of soybean seeds is simple and easy to implement, making the reagent for improving the germination rate of soybean seeds easy to promote and apply at low cost.
[0006] To achieve the above objectives, the present invention provides the following technical solution: The application provides a reagent for improving the germination rate of soybean seeds, which comprises 70-80 parts of L-glutamic acid, 4-6 parts of 6-benzyladenine and 1000 parts of water by weight.
[0007] The application finds that L-glutamic acid is low in cost and theoretically has good seed growth-promoting effect when selecting plant hormones, but it is found in experiments that L-glutamic acid alone has unstable promoting effect on the germination of soybean seeds. On this basis, it is found that when the input amount of 6-benzyladenine and L-glutamic acid is improper, the two growth-promoting plant hormones have antagonistic effect on the germination of soybean seeds. Only the reagent prepared in the range provided by the application has stronger growth-promoting effect than single L-glutamic acid of the same concentration and single 6-benzyladenine of the same concentration, and the germination rate of soybean seeds is significantly improved. This proves that, under a specific concentration, L-glutamic acid and 6-benzyladenine have synergistic growth-promoting effect.
[0008] Preferably, it comprises 75 parts of L-glutamic acid, 5 parts of 6-benzyladenine and 1000 parts of water by weight.
[0009] The application provides a method for improving the germination rate of soybean seeds, which comprises pretreating and disinfecting soybean seeds, then soaking the soybean seeds with the above-mentioned reagent for improving the germination rate of soybean seeds, drying the soybean seeds after soaking, and then performing germination treatment.
[0010] The seeds treated by the method of the application have good plant growth trend within 1 month after germination, and do not have any adverse phenomena such as deformity. This shows that the reagent for improving the germination rate of soybean seeds does not have negative effect on the subsequent growth and development of plants in the process of promoting the germination of soybean seeds, and ensures the healthy growth of plants, laying a solid foundation for high-yield and high-quality planting of soybeans.
[0011] Preferably, the soybean seeds are Nannong 66.
[0012] Preferably, the pretreatment is performed by selecting soybean seeds of uniform size, fullness and smooth surface without stains, soaking the soybean seeds in 75% ethanol for 1-3 min, and shaking the soybean seeds while soaking and then pouring off the ethanol.
[0013] The soybean seeds are shaken gently during soaking to ensure that all parts of the soybean seeds can be fully contacted with the ethanol, and then the ethanol is poured off. This step uses the sterilization property of ethanol to preliminarily disinfect the surface of the soybean seeds, so as to reduce possible microbial contamination in the subsequent germination process.
[0014] Preferably, the disinfection treatment is performed by placing the pretreated soybean seeds in a sodium hypochlorite solution with an effective chlorine concentration of 0.3-0.5 wt%, and performing oscillation treatment at 24-26℃ for 5-10 min.
[0015] The sodium hypochlorite solution has strong oxidizing property, can further kill harmful microorganisms on the surface of the soybean seeds and possibly in the soybean seeds, and creates a relatively clean environment for the germination of the soybean seeds, and then the container containing the soybean seeds and the sodium hypochlorite solution is placed in a shaking table for oscillation treatment.
[0016] The ethanol can quickly kill common microorganisms on the surface of the soybean seeds, and the low-concentration sodium hypochlorite solution can further remove potential bacteria, and because the concentration is low, the disinfection effect is ensured and the damage to the seeds is minimized. The combined disinfection method effectively solves the problem that the seeds are easily contaminated by bacteria and fungi during the germination process due to a long time, creates a relatively clean environment for the germination of the soybean seeds, and greatly improves the success rate of the germination of the soybean seeds.
[0017] Preferably, the soaking treatment is that the soybean seeds are washed with sterile water for 3-4 times, and then are placed in the above-mentioned soybean seed germination rate improving agent and soaked for 4-6 h at 24-26℃.
[0018] After the disinfection is completed, the soybean seeds are washed with sterile water for 3-4 times to remove the residual sodium hypochlorite solution.
[0019] Preferably, the germination treatment is direct sowing or germination with wet filter paper for 5-7 days at 24-26℃.
[0020] Therefore, the present application has the following beneficial effects: (1) The present application provides a soybean seed germination rate improving agent which can effectively promote the germination rate of the soybean seeds, and the seeds treated with the agent have a good growth trend and do not have any adverse phenomena such as deformity within one month after germination.
[0021] (2) The present application uses 6-benzyladenine to solve the defect that the L-glutamic acid has unstable growth effect on promoting germination, so that the compounded soybean seed germination rate improving agent shows more significant growth effect on promoting germination than single 6-benzyladenine or single L-glutamic acid.
[0022] (3) The present application uses specific concentrations of 6-benzyladenine and L-glutamic acid to compound the soybean seed germination rate improving agent, so that the two have a synergistic effect.
[0023] (4) The present application first uses 75% anhydrous ethanol and a sodium hypochlorite solution with an effective chlorine concentration of only 0.3% to disinfect the soybean seeds. The combined disinfection method effectively solves the problem that the seeds are easily contaminated by bacteria and fungi during the germination process due to a long time, creates a relatively clean environment for the germination of the seeds, and greatly improves the success rate of the germination of the seeds. Attached Figure Description
[0024] Figure 1 The effect of different concentrations of L-glutamic acid on soybean seed germination is shown in the figure. Figure 2 The figure shows the effect of different concentrations of L-glutamic acid on the root growth of soybean seeds. Figure 3 Figure showing the effect of synergistic treatment with L-glutamic acid and 6-benzyladenine on soybean seed germination; Figure 4 A growth chart of soybean plants one month after germination, treated with reagents. Detailed Implementation
[0025] The present invention will be further described below with reference to specific embodiments. Those skilled in the art will be able to implement the present invention based on these descriptions. Furthermore, the embodiments of the present invention described below are generally only some, not all, of the embodiments of the present invention. Therefore, all other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort should fall within the scope of protection of the present invention.
[0026] Example 1 1) Pretreatment: Select seeds of Nanjing 66 that are uniform in size, plump, smooth and free of blemishes, and have been stored for a long time with poor germination rate. Soak them in 75% ethanol for 1 minute, shaking them while soaking, and then pour off the ethanol.
[0027] 2) Disinfection treatment: Add the soybean seeds obtained in step 1) to a sodium hypochlorite solution with 0.3 wt% available chlorine, and place the container containing the soybean seeds and sodium hypochlorite solution at 25°C and shake for 5 min.
[0028] 3) Soaking treatment: Wash the soybean seeds from step 2) three times with sterile water, add a reagent to improve soybean seed germination rate, and soak at 25℃ for 4 hours. The final concentration of L-glutamic acid in the soybean seed germination rate improving reagent is 75 mg / L, and the final concentration of 6-benzyladenine (6-BA) is 5 mg / L.
[0029] 4) Germination Treatment: Discard the treatment solution from step 3). After the soybean seeds have dried at room temperature, sow them directly or germinate them on damp filter paper at 25°C for 5 days. Calculate the seed germination rate. The germination rate is calculated as follows: Germination rate = Number of germinated seeds / Total number of seeds * 100%; the calculations for the following examples and comparative examples are the same.
[0030] Comparative Example 1: CK Control Group 1) Pretreatment: Selecting the same size, full and smooth surface without stains, and seeds stored for a long time, poor germination rate of Nannong 66 seeds, soaking with 75% ethanol for 1 min, shaking while soaking, then pouring off the ethanol.
[0031] 2) Disinfection treatment: The soybean seeds obtained in step 1) were added with 0.3 wt% available chlorine sodium hypochlorite solution, and the container containing the soybean seeds and sodium hypochlorite solution was placed in 25℃ oscillation treatment for 5 min.
[0032] 3) Soaking treatment: The soybean seeds in step 2) were washed with sterile water for 3 times, deionized water was added, and 25℃ soaking for 4 h.
[0033] 4) Germination treatment: The treatment liquid in step 3) was poured off, the soybean seeds were dried at room temperature, then directly sowed or germinated at 25℃ for 5 days with wet filter paper, and the seed germination rate was counted.
[0034] Comparative Example 2 1) Pretreatment: Selecting the same size, full and smooth surface without stains, and seeds stored for a long time, poor germination rate of Nannong 66 seeds, soaking with 75% ethanol for 1 min, shaking while soaking, then pouring off the ethanol.
[0035] 2) Disinfection treatment: The soybean seeds obtained in step 1) were added with 0.3 wt% available chlorine sodium hypochlorite solution, and the container containing the soybean seeds and sodium hypochlorite solution was placed in 25℃ oscillation treatment for 5 min.
[0036] 3) Soaking treatment: The soybean seeds in step 2) were washed with sterile water for 3 times, deionized water was added, and 25℃ soaking for 4 h. In the soybean seed germination rate improving agent, the final concentration of L-glutamic acid was 75 mg / L.
[0037] 4) Germination treatment: The treatment liquid in step 3) was poured off, the soybean seeds were dried at room temperature, then directly sowed or germinated at 25℃ for 5 days with wet filter paper, and the seed germination rate was counted.
[0038] Comparative Example 3 1) Pretreatment: Selecting the same size, full and smooth surface without stains, and seeds stored for a long time, poor germination rate of Nannong 66 seeds, soaking with 75% ethanol for 1 min, shaking while soaking, then pouring off the ethanol.
[0039] 2) Disinfection treatment: The soybean seeds obtained in step 1) were added with 0.3 wt% available chlorine sodium hypochlorite solution, and the container containing the soybean seeds and sodium hypochlorite solution was placed in 25℃ oscillation treatment for 5 min.
[0040] 3) Soaking treatment: The soybean seeds in step 2) were washed with sterile water for 3 times, and a soybean seed germination rate improving agent was added, and soaked at 25°C for 4 h. In the soybean seed germination rate improving agent, the final concentration of L-glutamic acid was 150 mg / L.
[0041] 4) Germination treatment: The treatment solution in step 3) was discarded, and the soybean seeds were directly sown after air-drying at room temperature or were germinated at 25°C for 5 days with wet filter paper, and then the seed germination rate was counted.
[0042] Comparative Example 4 1) Pretreatment: Nannong 66 seeds with uniform size, fullness, smooth surface and no spot, and poor germination rate due to long storage were selected, soaked with 75% ethanol for 1 min, and then the ethanol was discarded while shaking.
[0043] 2) Disinfection treatment: The soybean seeds obtained in step 1) were added with 0.3 wt% available chlorine sodium hypochlorite solution, and the container containing the soybean seeds and the sodium hypochlorite solution was placed in a 25°C shaker for 5 min.
[0044] 3) Soaking treatment: The soybean seeds in step 2) were washed with sterile water for 3 times, and a soybean seed germination rate improving agent was added, and soaked at 25°C for 4 h. In the soybean seed germination rate improving agent, the final concentration of L-glutamic acid was 150 mg / L.
[0045] 4) Germination treatment: The treatment solution in step 3) was discarded, and the soybean seeds were directly sown after air-drying at room temperature or were germinated at 25°C for 5 days with wet filter paper, and then the seed germination rate was counted.
[0046] Comparative Example 5 1) Pretreatment: Nannong 66 seeds with uniform size, fullness, smooth surface and no spot, and poor germination rate due to long storage were selected, soaked with 75% ethanol for 1 min, and then the ethanol was discarded while shaking.
[0047] 2) Disinfection treatment: The soybean seeds obtained in step 1) were added with 0.3 wt% available chlorine sodium hypochlorite solution, and the container containing the soybean seeds and the sodium hypochlorite solution was placed in a 25°C shaker for 5 min.
[0048] 3) Soaking treatment: The soybean seeds in step 2) were washed with sterile water for 3 times, and a soybean seed germination rate improving agent was added, and soaked at 25°C for 4 h. In the soybean seed germination rate improving agent, the final concentration of L-glutamic acid was 150 mg / L.
[0049] 4) Germination treatment: The treatment solution in step 3) was discarded, and the soybean seeds were directly sown after air-drying at room temperature or were germinated at 25°C for 5 days with wet filter paper, and then the seed germination rate was counted.
[0050] Comparative Example 6 1) Pretreatment: Select Nannong 66 seeds of uniform size, full and smooth surface without stains, and seeds stored for a long time with poor germination rate, soak in 75% ethanol for 1 min, shake while soaking, then pour out the ethanol.
[0051] 2) Disinfection treatment: Add 0.3 wt% available chlorine sodium hypochlorite solution to the soybean seeds obtained in step 1), and place the container containing the soybean seeds and sodium hypochlorite solution in a 25°C shaking treatment for 5 min.
[0052] 3) Soaking treatment: Wash the soybean seeds in step 2) with sterile water 3 times, add a soybean seed germination rate improving agent, and soak at 25°C for 4 h. In the soybean seed germination rate improving agent, the final concentration of L-glutamic acid is 150 mg / L, and the final concentration of 6-benzyladenine is 5 mg / L.
[0053] 4) Germination treatment: Pour out the treatment solution in step 3), dry the soybean seeds at room temperature, then directly sow or use wet filter paper to germinate at 25°C for 5 days, and then count the seed germination rate.
[0054] Comparative Example 7 1) Pretreatment: Select Nannong 66 seeds of uniform size, full and smooth surface without stains, and seeds stored for a long time with poor germination rate, soak in 75% ethanol for 1 min, shake while soaking, then pour out the ethanol.
[0055] 2) Disinfection treatment: Add 0.3 wt% available chlorine sodium hypochlorite solution to the soybean seeds obtained in step 1), and place the container containing the soybean seeds and sodium hypochlorite solution in a 25°C shaking treatment for 5 min.
[0056] 3) Soaking treatment: Wash the soybean seeds in step 2) with sterile water 3 times, add a soybean seed germination rate improving agent, and soak at 25°C for 4 h. In the soybean seed germination rate improving agent, the final concentration of L-glutamic acid is 300 mg / L, and the final concentration of 6-benzyladenine is 5 mg / L.
[0057] 4) Germination treatment: Pour out the treatment solution in step 3), dry the soybean seeds at room temperature, then directly sow or use wet filter paper to germinate at 25°C for 5 days, and then count the seed germination rate.
[0058] Example 1, Comparative Examples 1-7 were repeated 3 times independently, and the germination was recorded and photographed. The corresponding seed germination rate results are recorded in Table 1.
[0059] Figure 1 The effect of different concentrations of L-glutamic acid on soybean seed germination, Figure 2The effect of L-glutamic acid with different concentrations on the growth of soybean seed roots; in combination with Table 1, it can be known that the promotion effect of L-glutamic acid with different concentrations on the germination of soybean seeds is unstable, but has a certain promotion effect on the growth of the roots of the germinated soybean seeds.
[0060] Figure 3 The effect of L-glutamic acid and 6-benzyladenine synergistic treatment on the germination of soybean seeds, it can be observed that 75 mg / L L-glutamic acid and 5 mg / L 6-benzyladenine (6-BA) can synergistically promote the germination of soybean seeds, and the germination rate is more significantly improved compared with 5 mg / L 6-benzyladenine alone. In addition, after single 150 mg / L L-glutamic acid (or 300 mg / L L-glutamic acid) and single 5 mg / L 6-benzyladenine treatment, the germination rate of the corresponding group of soybean seeds is stably improved compared with CK, while the combination of 150 mg / L L-glutamic acid (or 300 mg / L L-glutamic acid) and 5 mg / L 6-benzyladenine appears to inhibit seed germination. This shows that only at a specific L-glutamic acid and 6-benzyladenine dosage, the two can have a synergistic effect. And after adding 5 mg / L 6-benzyladenine, the promotion effect of the seed germination of the embodiment 1 group is more stable, which shows that 6-BA can well make up for the defect of unstable promotion effect of L-glutamic acid.
[0061] Figure 4 The growth trend of soybean plants of soybean seeds treated with reagents, it can be observed that the reagent (75 mg / L L-glutamic acid + 5 mg / L 6-benzyladenine) for improving the germination rate of soybean seeds provided by the application not only can promote seed germination, but also has a good growth trend within 1 month after germination, without any adverse phenomena such as malformation. This shows that the reagent for improving the germination rate of soybean seeds does not have a negative impact on the subsequent growth and development of plants in the process of promoting the germination of soybean seeds, ensuring the healthy growth of plants, and laying a solid foundation for high-yield and high-quality planting of soybeans.
[0062] Table 1 Comparison of seed germination rates under different treatment reagents
Claims
1. An agent for improving the germination rate of soybean seeds, characterized by comprising a peptide having the amino acid sequence of SEQ ID NO:
1. By weight parts, comprising: 70~80 parts of L-glutamic acid, 4~6 parts of 6-benzyladenine and 1000 parts of water.
2. The agent for improving the germination rate of soybean seeds according to claim 1, wherein By weight parts, comprising: 75 parts of L-glutamic acid, 5 parts of 6-benzyladenine and 1000 parts of water.
3. A method of increasing the germination rate of soybean seeds, characterized by, Comprising: After the soybean seeds are pretreated and disinfected, the soybean seeds are soaked with the reagent of claim 1 or 2, and then the soybean seeds are dried and germinated.
4. The method of claim 1, wherein, The soybean seeds are Nong 66.
5. The method of claim 1 or 4, wherein, The pretreatment is performed by selecting soybean seeds with uniform size, fullness and smooth surface, soaking the soybean seeds in 75% ethanol for 1~3 min, and shaking the soybean seeds while soaking.
6. The method of claim 1 or 4, wherein, The disinfection is performed by placing the pretreated soybean seeds in a sodium hypochlorite solution with an effective chlorine concentration of 0.3~0.5 wt%, and shaking the soybean seeds at 24~26℃ for 5~10 min.
7. The method of claim 1 or 4, wherein, The soaking is performed by washing the soybean seeds with sterile water for 3~4 times, and then placing the soybean seeds in the reagent of claim 1 or 2, and soaking the soybean seeds at 24~26℃ for 4~6 h.
8. The method of claim 1 or 4, wherein, The germination is performed by directly sowing the soybean seeds, or germinating the soybean seeds with wet filter paper at 24~26℃ for 5~7 days.