Traditional Chinese medicine freeze-dried powder for dispelling effects of alcohol and protecting liver and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicine, and provides traditional Chinese medicine freeze-dried powder for dispelling effects of alcohol and protecting liver and a preparation method thereof. The mass ratio of the freeze-dried powder of radix puerariae to the freeze-dried powder of semen hoveniae to the freeze-dried powder of schisandra chinensis is (0.8-1.2): (0.8-1.2): (0.8-1.2) The radix puerariae promotes alcohol metabolism, the hovenia dulcis thunb relieves alcohol toxicity, the schisandra chinensis protects the liver, the synergistic effect of the three traditional Chinese medicinal materials is fully played, the alcohol effect relieving and liver protecting effects are enhanced, the freeze drying technology is adopted, drying is completed under the low-temperature condition, damage of high temperature to active ingredients is avoided, and the stability of the product in the storage process is remarkably improved; the freeze-dried powder exists in a fine particle state, so that the contact surface area with a human body is increased, the release and absorption of effective components are facilitated, and the bioavailability is improved.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of traditional Chinese medicine, and particularly relates to a freeze-dried powder of a traditional Chinese medicine for dispelling alcohol and protecting liver and a preparation method thereof. BACKGROUND

[0002] The metabolism of alcohol in the human body after drinking mainly depends on the liver, and long-term or excessive drinking is easy to cause liver damage and induce alcoholic hepatitis, fatty liver and other diseases. Therefore, it is of great practical significance to develop safe and effective products for dispelling alcohol and protecting liver.

[0003] The products for dispelling alcohol and protecting liver on the market at present are mainly divided into two categories: one is a single traditional Chinese medicinal material extract preparation, such as a product taking pueraria extract as the core, whose principle is to use flavonoids in pueraria to promote alcohol metabolism, thereby reducing the burden on the liver; the other is a simple mixed preparation of multiple traditional Chinese medicinal materials, such as a compound product containing pueraria, jujube fruit and the like, which attempts to achieve the purpose of dispelling alcohol and protecting liver through the superposition of the effects of different medicinal materials. In the preparation process of these products, the extraction method is mostly traditional decoction or alcohol extraction, and the drying step mainly relies on spray drying, vacuum drying and the like.

[0004] However, the existing technology has obvious limitations and defects: firstly, the stability of effective components is poor. Traditional drying methods (such as spray drying and vacuum drying) need to be carried out under high temperature conditions, which is easy to cause the structure of active components (such as flavonoids and glycosides) in traditional Chinese medicinal materials to be destroyed or degraded due to high temperature, so that the content of effective components is easy to decrease and the activity is easy to lose during storage, which seriously affects the stability of product quality. Secondly, the bioavailability is low. The single traditional Chinese medicinal material extract product only relies on a single component to play a role, and the effect is single; and the simple mixed product of multiple traditional Chinese medicinal materials has not been optimized by scientific extraction process, and the compatibility of effective components is poor, which leads to the release and absorption efficiency in the body being not ideal, and the drug effect cannot be fully played. Thirdly, the synergistic effect is not obvious. The existing compound products are mostly direct mixing or simple combination after rough extraction of multiple traditional Chinese medicinal materials, and there is no in-depth research on the synergistic mechanism of effective components of each medicinal material, and the high-efficiency synergy between components cannot be achieved through precise formula design and step-by-step extraction process, so it is difficult to fully play the complementary effects of different traditional Chinese medicinal materials in dispelling alcohol and protecting liver, and the effect of dispelling alcohol and protecting liver is limited. Fourthly, the preparation process has defects. The traditional extraction method (such as single decoction or alcohol extraction) has a low extraction rate of effective components, which easily causes waste of raw materials; at the same time, the traditional drying technology not only has high energy consumption and long time consumption, but also needs to go through complex process control, which is not conducive to the improvement of industrial production efficiency. The core reason for these problems lies in the high-temperature limitation of traditional drying technology and the insufficient research on the characteristics of effective components and synergistic mechanism of the preparation process, which leads to the existing products being difficult to meet the needs of people for the effect of dispelling alcohol and protecting liver, product stability and convenience of taking. SUMMARY

[0005] To solve the above technical problems, the present application provides a kind of alcohol-protecting liver traditional Chinese medicine freeze-dried powder and its preparation method, to solve the effective component stability of existing alcohol-protecting liver product, low bioavailability, technical problems such as not obvious synergistic effect.

[0006] According to the first aspect of the present disclosure, an alcohol-protecting liver traditional Chinese medicine freeze-dried powder is provided, which is composed of Pueraria lobata freeze-dried powder, Hovenia acerba freeze-dried powder and Schisandra chinensis freeze-dried powder; the mass ratio of the Pueraria lobata freeze-dried powder, Hovenia acerba freeze-dried powder and Schisandra chinensis freeze-dried powder is (0.8-1.2):(0.8-1.2):(0.8-1.2).

[0007] Preferably, the mass ratio of the Pueraria lobata freeze-dried powder, Hovenia acerba freeze-dried powder and Schisandra chinensis freeze-dried powder is 1:1:1.

[0008] Preferably, the freeze-dried powder combination can be made into capsules, tablets or granules.

[0009] According to the second aspect of the present disclosure, a preparation method of an alcohol-protecting liver traditional Chinese medicine freeze-dried powder is also provided, which comprises the following steps:

[0010] S1, using Pueraria lobata, Hovenia acerba and Schisandra chinensis, after removing impurities, washing and drying, respectively, and crushing into powder;

[0011] S2, respectively extracting the Pueraria lobata powder, Hovenia acerba powder and Schisandra chinensis powder obtained in step S1 to obtain respective extraction solutions;

[0012] S3, filtering the respective extraction solutions obtained in step S2 to remove impurities, and then concentrating the filtered extraction solutions by vacuum concentration method to obtain respective concentrated solutions;

[0013] S4, freeze-drying the respective concentrated solutions obtained in step S3, which includes three stages of pre-freezing, sublimation drying and desorption drying;

[0014] S5, crushing the respective extracts after freeze-drying in step S4 to obtain freeze-dried powder, and then uniformly mixing the freeze-dried powder according to the mass ratio (0.8-1.2):(0.8-1.2):(0.8-1.2) to obtain the alcohol-protecting liver traditional Chinese medicine freeze-dried powder combination.

[0015] Preferably, in step S2, the extraction is performed by water decoction method or alcohol extraction method;

[0016] The water decoction method is performed by setting the water addition amount to 8-12 times the weight of the corresponding traditional Chinese medicinal materials, the decoction temperature to 80-100℃, the decoction time to 1-3 hours, and the extraction times to 2-3 times;

[0017] The alcohol extraction method uses ethanol with a concentration of 60%-80%, the extraction temperature is 40-60 DEG C, and the extraction time is 2-4 hours.

[0018] Preferably, in the step S4, the temperature of the pre-freezing stage is -40 DEG C to -30 DEG C, so that the temperature of the material is reduced below the eutectic point; the vacuum degree of the sublimation drying stage is 10-30 Pa, and the temperature is -20 DEG C to -10 DEG C; the vacuum degree of the desorption drying stage is 5-15 Pa, and the temperature is 20-30 DEG C.

[0019] Compared with the prior art, the present application has the following beneficial effects:

[0020] 1. The present application completes drying under low temperature conditions through freeze-drying technology, avoids damage to effective components caused by high temperature, and significantly improves the stability of the product during storage; the freeze-dried powder exists in the form of fine particles, increases the contact surface area with the human body, is beneficial to the release and absorption of effective components, and improves the bioavailability.

[0021] 2. The present application fully plays the synergistic effects of the three kinds of Chinese medicinal materials by promoting alcohol metabolism through Pueraria, removing alcohol toxicity through Hovenia dulcis Thunb, and protecting the liver through Schisandra chinensis, and enhances the effects of removing alcohol and protecting the liver; not only can quickly remove alcohol and reduce the immediate damage of alcohol to the liver, but also can improve the antioxidant capacity of the liver and prevent the occurrence of alcoholic liver disease when taken for a long time.

[0022] 3. The present application uses the extraction process combining water decocting method and alcohol extraction method, improves the extraction rate of effective components, and reduces the waste of raw materials; compared with the traditional drying method, the freeze-drying technology saves energy and time and simplifies the process. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 The present application is a preparation method flow chart of the alcohol-removing and liver-protecting Chinese medicinal freeze-dried powder. DETAILED DESCRIPTION

[0024] The embodiments of the present application will be further described in detail below in combination with the drawings and examples. The following examples are used to illustrate the present application, but cannot be used to limit the scope of the present application.

[0025] Example 1: Preparation of alcohol-removing and liver-protecting Chinese medicinal freeze-dried powder combination by water decocting method

[0026] Select 100g of high-quality Pueraria, 100g of Hovenia dulcis Thunb, and 100g of Schisandra chinensis, wash them with purified water after removing impurities, and dry them in a cool place; grind the three kinds of medicinal materials into 80-mesh powder after drying, and reserve them.

[0027] Pueraria extraction: take Pueraria powder, add 10 times the weight of purified water (i.e. 1000g), place in a decoction pot, heat to 90°C, maintain for 2 hours, filter to obtain the first extraction liquid; add 8 times the weight of purified water (800g) to the residue, heat to 95°C, maintain for 1.5 hours, filter to obtain the second extraction liquid; combine the two extraction liquids to obtain the Pueraria extraction liquid.

[0028] Hovenia extraction: take Hovenia powder, add 12 times the weight of purified water (1200g), heat to 100°C, maintain for 2.5 hours, filter to obtain the first extraction liquid; add 10 times the weight of purified water (1000g) to the residue, heat to 90°C for 2 hours, filter to obtain the second extraction liquid; combine the two extraction liquids to obtain the Hovenia extraction liquid.

[0029] Schisandra extraction: take Schisandra powder, add 8 times the weight of purified water (800g), heat to 85°C, maintain for 3 hours, filter to obtain the first extraction liquid; add 8 times the weight of purified water (800g) to the residue, heat to 85°C for 2 hours, filter to obtain the second extraction liquid; combine the two extraction liquids to obtain the Schisandra extraction liquid.

[0030] Filter the above Pueraria, Hovenia and Schisandra extraction liquids using a 0.45μm filter membrane to remove impurities; place the filtered extraction liquids in a vacuum concentration tank, concentrate to 1 / 5 of the original volume at 60°C and a vacuum degree of -0.08MPa to obtain the respective concentrated liquids.

[0031] Place the respective concentrated liquids in a freeze-drying tray (thickness of about 1cm) and place in a freeze-drying machine:

[0032] Pre-freezing stage: set the temperature to -35°C and maintain for 4 hours to completely freeze the material (temperature drops below the eutectic point);

[0033] Sublimation drying stage: turn on the vacuum system, maintain a vacuum degree of 20Pa, set the shelf temperature to -15°C, and maintain for 12 hours to sublimate the frozen water;

[0034] Desorption drying stage: adjust the vacuum degree to 10Pa, raise the shelf temperature to 25°C, and maintain for 6 hours to further remove residual water.

[0035] Grind the freeze-dried Pueraria, Hovenia and Schisandra extracts using a super-micro grinder to 100 mesh freeze-dried powder; take 50g of each of the three freeze-dried powders, mix in a mixer for 30 minutes to obtain a Jiuji Hugan traditional Chinese medicine freeze-dried powder combination.

[0036] Place the mixed freeze-dried powder in an aluminum-plastic composite bag, seal and store in a dry, cool, ventilated warehouse (relative humidity ≤60%, temperature ≤25°C).

[0037] Example 2: Preparation of the freeze-dried powder of the alcohol-protecting Chinese medicine for alcoholism by alcohol extraction

[0038] The same as Example 1, 100 g of Puerariae radix, 100 g of Hoveniae Fructus and 100 g of Schisandrae Fructus were selected, and then crushed into 80-mesh powder after treatment.

[0039] Puerariae radix extraction: Puerariae radix powder was taken, 70% ethanol (solid-liquid ratio 1:10, 1000 mL) was added, and the extraction tank was placed in a constant temperature oven at 50°C for 3 hours. The first extraction liquid was obtained by filtration. The residue was added with 70% ethanol (solid-liquid ratio 1:8, 800 mL), and extracted at 50°C for 2 hours. The second extraction liquid was obtained by filtration. The extraction liquids were combined, and the ethanol was recovered until no alcohol taste was left. Puerariae radix extraction liquid was obtained.

[0040] Hoveniae Fructus extraction: Hoveniae Fructus powder was taken, 60% ethanol (solid-liquid ratio 1:12, 1200 mL) was added, and the extraction tank was placed in a constant temperature oven at 45°C for 4 hours. The first extraction liquid was obtained by filtration. The residue was added with 60% ethanol (solid-liquid ratio 1:10, 1000 mL), and extracted at 45°C for 3 hours. The second extraction liquid was obtained by filtration. The extraction liquids were combined, and the ethanol was recovered. Hoveniae Fructus extraction liquid was obtained.

[0041] Schisandrae Fructus extraction: Schisandrae Fructus powder was taken, 80% ethanol (solid-liquid ratio 1:10, 1000 mL) was added, and the extraction tank was placed in a constant temperature oven at 60°C for 2.5 hours. The first extraction liquid was obtained by filtration. The residue was added with 80% ethanol (solid-liquid ratio 1:8, 800 mL), and extracted at 60°C for 2 hours. The second extraction liquid was obtained by filtration. The extraction liquids were combined, and the ethanol was recovered. Schisandrae Fructus extraction liquid was obtained.

[0042] The same as Example 1, the extraction liquid was filtered through a 0.45 μm filter membrane, and then concentrated to 1 / 5 of the original volume under the conditions of 60°C and a vacuum degree of-0.08 MPa.

[0043] The same as Example 1, the pre-freezing temperature was-35°C (4 hours), the sublimation drying vacuum degree was 20 Pa, the sublimation drying temperature was-15°C (12 hours), the resolution drying vacuum degree was 10 Pa, and the resolution drying temperature was 25°C (6 hours).

[0044] The three kinds of extracts were crushed into 100-mesh freeze-dried powder, and then mixed uniformly according to the mass ratio of 1:1:1 to obtain the target product.

[0045] The mixed freeze-dried powder was packed into aluminum-plastic composite bags, sealed and packaged, and stored in a dry, cool, ventilated warehouse (relative humidity≤60%, temperature≤25°C).

[0046] Through the optimized extraction process and freeze-drying technology, a Chinese medicine freeze-dried powder composition with high content of effective components and good stability can be stably prepared. The product can be further prepared into capsules (each containing 0.3 g of freeze-dried powder), tablets (each containing 0.5 g of freeze-dried powder) or granules (each containing 1 g of freeze-dried powder) through conventional preparation process, which are convenient to take. In actual production, the process conditions can be adjusted within the range of the parameters defined in the application according to the characteristics of the raw materials and production needs.

[0047] Detection method and experimental results

[0048] Detection method: the content of effective components can be detected by high performance liquid chromatography and the like. The alcohol-eliminating and liver-protecting effect of the product can be detected by animal experiments and the like, such as observing the behavior changes of animals after drinking, detecting the alcohol concentration in blood and liver-related indicators, etc.

[0049] Experimental results: the expected experimental results show that the product can significantly reduce the alcohol concentration in blood, reduce liver damage indicators, and improve the antioxidant capacity of liver, etc.

[0050] Importantly, it should be noted that the constructions and arrangements of the present application shown in the various different exemplary embodiments are illustrative only. Although only a few embodiments have been described in detail in this disclosure, those skilled in the art who review this disclosure will readily appreciate that many modifications are possible (e.g., variations in sizes, dimensions, structures, shapes and proportions of the various elements, values of parameters, mounting arrangements, use of materials, colors, orientations, etc.) without materially departing from the novel teachings and advantages of the subject matter described in this application. For example, elements shown as integrally formed can be constructed of multiple parts or elements, the position of elements can be reversed or otherwise varied, and the nature or number of elements or positions can be altered or varied. Accordingly, all such variations are intended to be included within the scope of this application. The order or sequence of any process or method steps can be changed or re-sequenced without departing from the scope of the application. In the claims, any "means plus function" clause is intended to cover the structures described herein as performing the recited functionality, and not only structural equivalents but also equivalent structures. Other substitutions, modifications, changes, and omissions can be made in the design, operating conditions, and arrangement of the exemplary embodiments without departing from the scope of the application. Accordingly, the present application is not limited to particular embodiments described, but extends to various modifications, combinations, sub-combinations, and equivalents obvious to those skilled in the art, following in the spirit of the application and the scope of the appended claims.

[0051] Furthermore, in order to provide a concise description of exemplary embodiments, all features of an actual implementation can not be described (i.e., those unrelated to the best mode of carrying out the application that is currently being considered, or those unrelated to enabling the application).

[0052] It is to be understood that the detailed description and specific examples described above are intended for purposes of illustration only and are not intended to limit the scope of the present application. Those of ordinary skill in the art will recognize that the application described herein can be practiced with modification and alteration, and that the present application is not limited to the above-described embodiments and examples. Accordingly, the specification is to be regarded in an illustrative rather than a restrictive sense.

[0053] It should be noted that the above examples are merely used to illustrate the technical solutions of the present application, rather than limit the present application. Although the present application is described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or equivalently replaced, without departing from the spirit and scope of the technical solutions of the present application, which should be covered in the scope of the claims of the present application.

Claims

1. A kind of traditional Chinese medicine freeze-dried powder for dispelling alcohol and protecting liver, characterized by: The freeze-dried powder is composed of Puerariae radix, Hoveniae fructus and Schisandrae chinensis fructus; the mass ratio of the Puerariae radix, Hoveniae fructus and Schisandrae chinensis fructus is (0.8-1.2):(0.8-1.2):(0.8-1.2).

2. The alcoholism-relieving and liver-protecting traditional Chinese medicine freeze-dried powder according to claim 1, characterized in that: The mass ratio of the Puerariae radix, Hoveniae fructus and Schisandrae chinensis fructus is 1:1:

1.

3. The alcoholism-relieving and liver-protecting traditional Chinese medicine freeze-dried powder according to claim 1, characterized in that: The freeze-dried powder combination can be made into capsules, tablets or granules.

4. A preparation method of a Chinese medicine freeze-dried powder for protecting liver and relieving alcoholism, characterized in that, The method comprises the following steps: S1, using Puerariae radix, Hoveniae fructus and Schisandrae chinensis fructus, removing impurities, washing and drying, and crushing into powder respectively; S2, extracting the Puerariae radix powder, Hoveniae fructus powder and Schisandrae chinensis fructus powder obtained in step S1 respectively to obtain respective extract; S3, filtering the respective extract obtained in step S2 to remove impurities, and then concentrating the filtered extract by using a reduced pressure concentration method to obtain respective concentrated liquid; S4, freeze-drying the respective concentrated liquid obtained in step S3, wherein the freeze-drying comprises three stages of pre-freezing, sublimation drying and desorption drying; S5, crushing the respective extract after freeze-drying in step S4 to obtain freeze-dried powder, and then mixing the freeze-dried powder uniformly according to the mass ratio (0.8-1.2):(0.8-1.2):(0.8-1.2) to obtain the freeze-dried powder combination of the alcohol-eliminating and liver-protecting traditional Chinese medicine.

5. The preparation method of the alcoholism-relieving and liver-protecting traditional Chinese medicine freeze-dried powder according to claim 4, characterized in that: In step S2, the extraction is performed by using a water decoction method or an alcohol extraction method; In the water decoction method, the water addition amount is 8-12 times the weight of the corresponding traditional Chinese medicine, the decoction temperature is 80-100℃, the decoction time is 1-3 hours, and the extraction times is 2-3 times; In the alcohol extraction method, 60%-80% ethanol is used, the extraction temperature is 40-60℃, and the extraction time is 2-4 hours.

6. The preparation method of the alcoholism-relieving and liver-protecting traditional Chinese medicine freeze-dried powder according to claim 4, characterized in that: In step S4, the temperature in the pre-freezing stage is-40℃ to-30℃, so that the material temperature is reduced below the eutectic point; the vacuum degree in the sublimation drying stage is 10-30 Pa, and the temperature is-20℃ to-10℃; the vacuum degree in the desorption drying stage is 5-15 Pa, and the temperature is 20-30℃.

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