Flexible adaptive probe assembly, cell operation system and filling method of probe reagent

Abstract

The invention discloses a flexibly-adaptive probe assembly, a cell operation system and a probe reagent filling method, and the flexibly-adaptive probe assembly comprises a slender probe of a hollow structure, and the probe is provided with a head part and a tail part which are opposite to each other; the probe seat is used for positioning the probe and is provided with a filling channel communicated with the tail part; and the sheath is detachably connected with the probe seat and is provided with an accommodating cavity for accommodating the head part. According to the technical scheme disclosed by the invention, through integral storage and use of the probe and the probe seat, a stable control basis is provided for the probe, and a structural basis is provided for improvement of a probe use environment while the risk of probe damage is reduced and the operation experience is improved.

Description

This application is a divisional application of application number 202510954457.4, the parent application information is as follows: Application number: 202510954457.4; Filing date: July 11, 2025; Application title: Flexible probe assembly, cell operation system and probe reagent loading method. TECHNICAL FIELD

[0001] The present application relates to the field of cell operation, in particular to a flexible probe assembly, a cell operation system and a probe reagent loading method. BACKGROUND

[0002] When conducting cell research, the specific morphology of the cells needs to be observed, and the target cells are extracted separately for research. Cell operation is generally achieved through a cell probe. In order to achieve micron or nanometer level operation tips, the cell probe is generally a capillary made of quartz, glass or other materials, which is drawn by laser heating. The front end of the cell probe is provided with a nanometer or micrometer opening to realize the extraction, release and injection of cells.

[0003] In order to realize the storage and transportation of the probe, the probe in the prior art is generally constrained in an independent package. When it is needed to be used, it is taken out of the package and installed and connected through multiple steps, which is cumbersome and has the risk of probe damage, and there is room for improvement. SUMMARY

[0004] In order to solve the above technical problems, the present application discloses a flexible probe assembly. The probe can be flexibly adapted to the sheath and the operation device through the probe holder, effectively avoiding the scene of directly operating the probe in the prior art, and reducing the risk of probe damage.

[0005] In an embodiment of the present application, a flexible probe assembly is disclosed, comprising: an elongated and hollow probe, the probe having opposite head and tail portions; a probe holder for positioning the probe, the probe holder having a loading channel communicating with the tail portion; and a sheath, the sheath being separably connected with the probe holder and having a receiving cavity for receiving the head portion.

[0006] The following also provides several optional modes, but not as an additional limitation to the above general scheme, only as a further supplement or preference, without technical or logical contradiction, each optional mode can be combined with the above general scheme, and the optional modes can also be combined.

[0007] In one embodiment, the probe assembly further comprises a spacer disposed in the receiving cavity for preventing the sheath from contacting the probe during the separation process.

[0008] In one embodiment, one end of the spacer is a fixed end constrained by the probe holder, and the other end is a holding end for holding the probe.

[0009] In one embodiment, the spacer provides a spacer cavity, and the spacer cavity is provided with an open window for the probe and the probe holder to enter and exit the spacer cavity laterally.

[0010] In one embodiment, the fixed end of the spacer is provided with a through hole for the probe holder to pass through. During the installation process, at least a portion of the probe holder extends to the outside of the spacer cavity after passing through the through hole from the spacer cavity. During the separation process, the probe holder is withdrawn to the spacer cavity from the through hole and exits from the open window.

[0011] In one embodiment, the spacer cavity has a limiting portion for limiting the probe holder from being withdrawn to a limit position from the through hole.

[0012] In one embodiment, the inner diameter of the side of the spacer cavity close to the probe holder is greater than the inner diameter of the side of the spacer cavity close to the head to provide the limiting portion.

[0013] In one embodiment, the spacer is provided with a holding groove communicated with the open window. During the installation process, the middle part of the probe enters and is accommodated in the holding groove. During the separation process, the middle part of the probe exits the holding groove to achieve separation from the spacer.

[0014] In one embodiment, the holding groove and the middle part of the probe are clearance fit.

[0015] In one embodiment, the outer peripheral surface of the spacer is clearance fit with the inner peripheral surface of the sheath.

[0016] In one embodiment, the probe assembly further comprises a base separably connected with the probe holder, and the base is separably connected with the sheath.

[0017] In one embodiment, the probe assembly further comprises a spacer disposed in the receiving cavity, and the spacer is clamped and positioned by the probe holder and the base.

[0018] In one embodiment, the sheath is connected with the probe holder through the base, and the base is provided with a filling hole for opening the filling channel.

[0019] In one embodiment, the probe holder has one end of a mounting section matched with the operating device or the base, and the other end of a connecting section matched with the probe, the mounting section is smaller in diameter than the connecting section, and a positioning shoulder is arranged between the mounting section and the connecting section, the base and the positioning shoulder are matched with each other to position the isolation frame.

[0020] In one embodiment, a gap is arranged between the outer periphery of the connecting section and the inner periphery of the isolation frame.

[0021] In one embodiment of the present application, a cell operation system is also disclosed, which comprises an operating device and the flexible probe assembly as described in the above technical solutions. The probe assembly has a use state and a storage state; in the storage state, the probe is positioned and connected with the sheath through the probe holder, and the head of the probe is suspended in the inside of the receiving cavity; in the use state, the probe holder is separated from the sheath, and the probe is connected to the operating device through the probe holder. The operating device comprises a stage for carrying a sample, a control assembly for moving the probe relative to the stage, a microscopic imaging assembly for providing a view window for the probe, and a spitting and taking assembly, the probe in the use state is connected to the control assembly through the probe holder, the control assembly positions the distal end of the probe to a preset position through the control of the holder, and the spitting and taking assembly drives the reagent to enter or exit the distal opening of the probe.

[0022] In one embodiment of the present application, a filling method of a probe reagent is also disclosed, which comprises providing a pipette and the probe assembly as described in the above technical solutions, and injecting the reagent into the inner cavity of the probe through the filling channel by the pipette; The probe assembly is placed as a whole in a centrifugal device for centrifugal treatment, so that the reagent moves to the head of the probe.

[0023] The technical solutions disclosed in the present application provide a stable control basis for the probe through the overall storage and use of the probe and the probe holder, reduce the risk of damage to the probe, improve the operation experience, and provide a structural basis for the improvement of the use environment of the probe.

[0024] The specific beneficial technical effects will be further explained in the specific embodiments in combination with specific structures or steps. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 The figure is a structural schematic diagram of the probe assembly in one embodiment of the present application; Figure 2 The figure is a structural schematic diagram of the probe assembly without the sheath in one embodiment of the present application; Figure 3 for Figure 2 The diagram shows the assembly of the probe components. Figure 4 This is a schematic diagram showing the probe assembly facing the base from one side. Figure 5 for Figure 4 A schematic diagram of the internal components of the probe assembly from the cross-sectional view at point AA. Figure 6 for Figure 5 A schematic diagram of the interaction between the liquid transfer gun and the probe assembly from a downward perspective; Figure 7 This is a schematic diagram of a pipette filling reagents into the probe. Figure 8 This is a schematic diagram showing the distribution of the reagent inside the probe after it moves upward along the probe axis under centrifugal force. Figure 9 for Figure 4 A schematic diagram of the internal components of the probe assembly from the cross-sectional view at point BB; Figure 10 and Figure 11 Schematic diagram of the probe, probe holder, and isolation frame at different stages of the separation process; Figure 12 for Figure 11 A schematic diagram of the engagement of the probe, probe holder, and isolation frame from a three-dimensional perspective; Figure 13 This is a schematic diagram of the cell operating system framework in one embodiment of this application; Figure 14 This is a schematic diagram of the probe reagent loading method in one embodiment of this application.

[0026] The annotations in the figure are explained as follows: 10. Stage; 21. Optical microscope objective; 22. Image detection unit; 23. Light source; 50. Moving platform; 60. Air pressure source assembly; 70. Control system; 100. Probe; 101. Head; 102. Tail; 110. Probe mount; 111. Loading channel; 112. Positioning shoulder; 300, Sheath; 301, Receiving cavity; 310, Base; 311, Filling hole; 312, Cylinder; 313, Disc; 320, Isolation frame; 321, Fixed end; 3211, Through hole; 322, Holding end; 3221, Holding groove; 323, Isolation cavity; 324, Reduced diameter structure; 901. Pipette. Detailed Implementation

[0027] With reference to the drawings, the technical solutions in the embodiments of the present application will be clearly and completely described, obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0028] It should be noted that when a component is referred to as being "connected" with another component, it can be directly connected with the other component or there can be a middle component. When a component is referred to as being "disposed on" another component, it can be directly disposed on the other component or there can be a middle component.

[0029] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used in the description herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items.

[0030] In the prior art, before the probe is ready, the operator needs to carefully take the probe out of the package and position the probe on a specific holder, calibrate the relative spatial position between the probe and the operation device, and then perform the corresponding cell operation. In the scenario where the probe needs to be filled with reagent, the probe needs to be positioned on a specific jig after being taken out, and then the probe is positioned on a specific holder after the reagent is filled into the probe by a pipette. The overall operation process is complex, tedious, has the risk of probe damage, requires high operation skills, and has low operation efficiency.

[0031] In order to overcome the above technical problems, the present application discloses a flexible and adaptive probe assembly, which comprises a probe 100, a probe seat 110 connected to the probe 100, and a sheath 300 connected to the probe seat 110. The probe seat 110 provides a stable control basis for the probe 100, reduces the risk of damage to the probe 100, improves the operation experience, and allows the probe 100 to be stably and quickly adapted to different scenarios, and provides a structural basis for improvement of different use environments.

[0032] Specifically, refer to the drawings Figure 1 to the drawings Figure 3The embodiment shown discloses a flexible probe assembly. The probe 100 is an elongated and hollow structure, and has opposite head 101 and tail 102. The probe holder 110 positions the probe 100, and has a loading channel 111 communicating with the tail 102, which is in communication with the interior of the probe 100. The sheath 300 is detachably connected with the probe holder 110, and has a receiving cavity 301 accommodating the head 101. When the sheath 300 and the probe holder 110 are connected with each other, the probe 100 is located in the sheath 300. The probe 100 can be operated by exposing the loading channel 111 to the outside in the case of removing the sheath 300, thereby reducing the scene that the probe 100 needs to be exposed, facilitating operation and reducing the risk of damage.

[0033] Reference is made to the accompanying drawings Figure 1 As shown, at this time the probe assembly is in a storage state, the probe 100 is positioned and connected by the probe holder 110 and the sheath 300, and the head 101 of the probe 100 is suspended inside the receiving cavity 301; when the sheath 300 and the probe holder 110 are separated from each other, the probe 100 is exposed to the external environment from the sheath 300, and reference is made to the accompanying drawings Figure 11 and the accompanying drawings Figure 13 As shown, at this time the probe assembly is in a use state, the probe holder 110 is separated from the sheath 300, and the probe 100 is connected to the operating device by the probe holder 110.

[0034] The specific structure of the sheath 300 is shown in the accompanying drawings Figure 3 As shown, the sheath 300 is a cylindrical structure. Specifically, the sheath 300 is open at one end and closed at the other end. The sheath 300 extends from the head 101 of the probe 100 to the tail 102 of the probe 100 to protect the probe 100. The sheath 300 can directly cooperate with the probe holder 110, or can cooperate with other components, such as the accompanying drawings Figure 3 to the accompanying drawings Figure 5As shown in the figure, the probe assembly further comprises a base 310 detachably connected with the probe holder 110, the sheath 300 is connected with the probe holder 110 through the base 310, and the base 310 is provided with a filling hole 311 of the open filling channel 111. In detail, the base 310 is provided with a first connecting structure radially inside and a second connecting structure radially outside, wherein the first connecting structure cooperates with the probe holder 110, and the second connecting structure cooperates with the sheath 300. The above-mentioned first connecting structure and second connecting structure can be realized by one or more mechanical positioning connection modes such as screwing, clamping, adsorption, etc. The base 310 is an integral structure and is divided into a cylinder body 312 for providing the connecting structure and a disc body 313 located on one side of the cylinder body 312, and the filling hole 311 penetrates the cylinder body 312 and the disc body 313. The outer edge of the cylinder body 312 cooperates with the probe holder 110 to limit the limit cooperation position of the base 310 and the probe holder 110, and the end face of the disc body 313 cooperates with the sheath 300 to limit the limit cooperation position of the base 310 and the sheath 300. The outer peripheral surface of the sheath 300 is further provided with a driving part for driving the sheath 300 to move relative to the base 310. Under the action of the driving part, the sheath 300 approaches or moves away from the base 310, and correspondingly, the probe 100 enters or exits the containing cavity 301.

[0035] Through the determined position relationship between the probe holder 110 and the sheath 300, the probe 100 can be stably suspended in the containing cavity 301. In order to further improve the stability of the probe 100 to avoid the influence of external force in storage and transportation and specific operation process, the probe assembly further comprises an isolation frame 320 arranged in the containing cavity 301. The isolation frame 320 can avoid the sheath 300 from contacting the probe 100 during separation. Referring to the accompanying Figure 3In the shown embodiment, the isolation frame 320 has a fixed end 321 which is limited by the probe holder 110 and a holding end 322 which holds the probe 100. The holding has various implementations, for example, the holding end 322 limits the radial position of the probe 100 away from the tail 102, for example, the holding end 322 cooperates with the outer surface of the probe 100, etc. In the above-mentioned manners, the holding end 322 and the outer surface of the probe 100 are in contact or in clearance. In the embodiment with the base 310, the isolation frame 320 is positioned by the probe holder 110 and the base 310. In order to ensure the independence of the isolation frame 320 during the cooperation of the components, the isolation frame 320 is only positioned in space by the fixed end 321 with the probe holder 110. Specifically, the inner surface of the isolation frame 320 is in clearance with the outer surface of the probe holder 110 (specifically, the connecting section of the probe holder 110 mentioned below). Further, the outer surface of the isolation frame 320 is in clearance with the inner surface of the sheath 300. The above-mentioned arrangement makes the sheath 300 not affect the isolation frame 320 and the probe 100 during the movement, thereby further reducing the risk of damage. Specifically, the holding end 322 acts on the middle part of the probe 100. In different embodiments, the holding end 322 can be selected to directly act on the outer surface of the probe 100 to achieve positioning, or can be selected to be in stress-free contact or clearance with the probe 100 and only suppress the radial movement trend of the probe 100 when the trend occurs. In the attached Figure 9 to the attached Figure 12 In the shown embodiment, the isolation frame 320 is provided with a holding groove 3221, the middle part of the probe 100 enters and is accommodated in the holding groove 3221, the side wall of the holding groove 3221 is in stress-free contact or clearance with the outer surface of the probe 100, and the holding groove 3221 is radially open to allow the probe 100 to enter or exit. Further, the holding groove 3221 is arranged on the holding end 322 and is communicated with the open window.

[0036] In order to reduce the risk of damaging the probe 100 during the cooperation and separation of the probe 100 and the isolation frame 320, reference is made to the attached Figure 9 to the attached Figure 12In the illustrated embodiment, during the switching between the storage state and the use state of the probe assembly, the distal end of the probe 100 never crosses the holding end 322 in the extension direction of the probe 100. Specifically, the isolation frame 320 provides an isolation cavity 323, and the side wall of the isolation frame 320 has an open window for the probe 100 and probe seat 110 to laterally enter and exit the isolation cavity 323. The fixed end 321 of the isolation frame 320 has a through hole 3211 for the probe seat 110 to pass through. During installation, at least a portion of the probe seat 110 (e.g., the mounting section of the probe seat 110 mentioned below) extends from the isolation cavity 323 through the through hole 3211 to the outside of the isolation cavity 323 and connects to the base 310; during separation, the probe seat 110 retracts from the through hole 3211 into the isolation cavity 323 and exits through the open window. Simultaneously, the retaining groove 3221 connects to the open window. During installation, the middle part of the probe 100 enters and is received within the retaining groove 3221; during separation, the middle part of the probe 100 exits the retaining groove 3221 to separate from the isolation frame 320. In the above process, the probe 100 and the probe seat 110 have a first axial movement and a second radial movement. These combined actions achieve the cooperation and separation of the probe 100, the probe seat 110, and the isolation frame 320. To facilitate the entry and exit of the probe 100 and the probe seat 110 from the isolation frame 320, the fixed end 321 and the retaining end 322 are connected by multiple strip-shaped connecting rods to form a frame structure. The interior of the frame structure is an isolation cavity 323, and the gaps between the strip-shaped connecting rods form open windows. To limit the relative movement of the probe holder 110 and the probe 100 relative to the isolation frame 320, the isolation cavity 323 has a limiting portion that restricts the probe holder 110 from retracting from the through hole 3211 to its limit position. (See attached diagram) Figure 5 In the illustrated embodiment, the inner diameter of the isolation cavity 323 near the probe holder 110 is larger than the inner diameter of the isolation cavity 323 near the head 101, to provide a limiting portion. That is, the inner diameter of the isolation cavity 323 decreases from the fixed end 321 to the holding end 322, preventing the probe holder 110 from moving to the holding end 322. This arrangement can be achieved through the spacing between the strip-shaped connecting rods. (Refer to the attached diagram.) Figure 12 As shown, in the extension direction of the probe 100, the isolation frame 320 has a reduced diameter structure 324, which provides a limiting portion by reducing the inner diameter of the isolation cavity 323.

[0037] Independent of the embodiments described above, the cooperative arrangement of the probe and the isolation frame in this application can also be understood as disclosing a probe assembly that is easy to assemble, including: A slender, hollow probe with a head and a tail. The probe holder for locating the probe; A sheath detachably connected to the probe holder, having a receiving cavity for accommodating the head; and a separation frame located in the receiving cavity, the separation frame providing a separation cavity, and the side wall of the separation frame being provided with an open window for the probe and the probe holder to enter and exit the separation cavity laterally; During the installation process, the probe holder and at least a part of the probe enter the separation cavity laterally through the open window and are kept positioned; During the separation process, the probe holder and the probe are disengaged and exit the separation cavity laterally from the open window.

[0038] The separation frame 320 can be connected with any one or both of the probe holder 110 and the base 310 to achieve its own holding effect. Referring to the embodiment shown in FIG. 1, the separation frame 320 is connected with the probe holder 110 and the base 310. Figure 3 In the embodiment shown, one end of the probe holder 110 is an installation section for cooperating with the operating device or the base 310, and the other end is a connection section for cooperating with the probe 100. The installation section is reduced in diameter compared to the connection section, and a positioning shoulder 112 is arranged between the installation section and the connection section. The base 310 and the positioning shoulder 112 clasp and position the fixed end 321 of the separation frame 320. The above arrangement can simplify the assembly relationship between the components and improve the use experience. After the base 310 and the probe holder 110 are separated, the separation between the probe holder 110 and the separation frame 320 can be automatically achieved.

[0039] The focus of the present application is the overall storage and use of the probe 100 and the probe holder 110, which provides a stable control basis for the probe 100. For example, one embodiment of the present application also discloses a cell operation system, as shown in FIG. 2. Figure 13 The cell operation system includes an operating device and the flexible and adaptive probe assembly in the above technical solution, which has the storage state and the use state mentioned above.

[0040] The operating device includes a stage 10 for carrying a sample, a control assembly for moving the probe 100 relative to the stage, a microscopic imaging assembly for providing a view window for the probe 100, and a spitting and sucking assembly. The probe 100 in the use state is connected to the control assembly through the probe holder 110. The control assembly positions the head 101 of the probe 100 to a preset position by controlling the probe holder 110. The spitting and sucking assembly drives a reagent to enter or exit the distal opening of the probe 100. The spitting and sucking assembly can be a field source assembly for providing an electric field / magnetic field, which drives the reagent to enter or exit the probe 100 under the action of the electric field / magnetic field. The spitting and sucking assembly can also be the air pressure source assembly 60 shown in FIG. 3. Figure 13 The air pressure source assembly 60 communicates with the probe 100 through the holder and the probe holder 110 to provide positive and negative air pressure to the probe 100 in the form of pulses. The air pressure source assembly 60 serves as a power source for sucking and spitting substances (cells, reagents, etc.).

[0041] The microscopic imaging assembly includes a light source 23, an optical microscope objective 21, and an image detection unit 22 (such as a CCD camera). The light source is generally positioned directly above the stage 10, while the optical microscope objective 21 and the image detection unit 22 are located below the stage. The control system 70 can be a terminal device such as a computer. The pneumatic source assembly 60, the motors in the moving platform 50 of the control assembly, and the motors in the stage 10 are controlled by this control system. The control system also has an imaging display unit, meaning that the probe 100 and the target cells can be displayed on the screen through the imaging system, allowing the operator to perform real-time operations.

[0042] As mentioned above, the probe assembly in this application also provides a structural basis for improving the usage environment of probe 100. For example, in scenarios where reagents need to be filled into probe 100, the process of removing probe 100 from its packaging and then repositioning it for filling is cumbersome. In this application, "reagents" refers to various liquid or gaseous media that can participate in cell manipulation, such as dyes, proteins, mRNA, plasmids, and other commonly injected substances in the field of cell manipulation; and also, for example, physiological saline, drugs, viruses, etc., that act on cells. Reagents can be a single substance or a combination of multiple substances. One embodiment of this application discloses a probe assembly that is easy to fill, having a distal end (on the side of probe 100 closer to probe seat 110 in the axial direction) and a proximal end (on the side of probe 100 farther from probe seat 110 in the axial direction), see attached... Figure 1 As shown, the device includes a probe 100, a probe holder 110, and a sheath 300. The probe 100 is an elongated hollow structure, with its distal end narrowing compared to its proximal end. The proximal end of the probe 100 is connected and positioned to the probe holder 110, which has a through-hole filling channel 111 communicating with the inner cavity of the probe 100. The sheath 300 extends from the distal end of the probe 100 to the probe holder 110, providing a closed cavity for housing the probe 100. The filling channel 111 communicates with the outside of this closed cavity. In this embodiment, the sheath 300 itself serves as the positioning component during the probe 100 filling operation, allowing for reagent filling without separating the probe 100 and the sheath 300, effectively improving filling efficiency and reducing the risk of probe 100 damage.

[0043] During reagent loading, the pipette 901 is typically used to fill the probe 100. However, due to the extremely small internal size of the probe 100, the pipette 901 cannot completely fill the probe 100, especially the space at the probe head, which poses a challenge for subsequent cell manipulation. To overcome these problems, in conjunction with the attached... Figure 14 One embodiment of this application also discloses a method for filling probe reagents, including... The probe assembly is provided with a pipette, and the pipette injects reagent into the inner cavity of the probe 100 through the filling channel 111. The probe assembly is placed in a centrifugal device for centrifugal treatment, so that the reagent moves to the head of the probe.

[0044] The cooperation between the centrifugal device and the probe assembly can be achieved by the sheath 300, which is positioned on the centrifugal device to avoid direct exposure of the probe 100 to the centrifugal device. The centrifugal force of the centrifugal device drives the reagent to move in the axial direction of the probe 100. The movement of the reagent in the axial direction of the probe 100 is specifically manifested as movement towards the head 101 of the probe 100 (a region with a smaller inner cavity size), so as to achieve complete filling of the inside of the probe 100. In the embodiment provided with the isolation frame 320, the isolation frame 320 can assist in positioning (for example, the above-mentioned support) of the probe 100 to resist the influence of the centrifugal force of the centrifugal device on the probe 100.

[0045] The above-mentioned filling method of the probe reagent achieves driving of the reagent in the probe 100 by the centrifugal device, and the sheath 300 of the probe assembly provides a stable positioning basis for centrifugation of the probe 100. The above-mentioned filling and centrifugation process can be achieved in a state where the probe 100, the probe seat 110 and the sheath 300 are not separated, which effectively optimizes the operation process and improves the use experience compared with the prior art.

[0046] Further, one embodiment of the present application also discloses an injection method for cell operation, comprising Providing a centrifugal device, an operation device and the above-mentioned probe assembly facilitating filling; Positioning the probe 100 and the probe seat 110 on the centrifugal device by the sheath 300, and driving the reagent of the probe 100 to move in the axial direction of the probe 100 to a preset position under the centrifugal force of the centrifugal device; Separating the probe assembly from the centrifugal device and separating the probe seat 110 and the probe 100 from the sheath 300; Mounting the probe 100 on the control assembly of the operation device by the probe seat 110, and controlling the operation device to position the distal end of the probe 100 to a preset position and drive the reagent to enter or exit through the distal end of the probe 100.

[0047] In the embodiment, the sheath 300 is used to achieve the cooperation between the probe 100 and the probe seat 110 and the centrifugal device, and the probe 100 and the operation device are directly cooperated by the probe seat 110. Therefore, from another perspective, one embodiment of the present application also discloses a cell operation system, comprising a centrifugal device, an operation device and the above-mentioned probe assembly; The centrifugal device comprises a rotating table and a positioning cavity arranged on the rotating table, the probe 100 is matched with the positioning cavity through the sheath 300, and the rotating table is used for providing a centrifugal force to drive the reagent of the probe 100 to move in the axial direction of the probe 100. The operation device comprises a carrier table for carrying a sample, a control assembly for moving the probe 100 relative to the carrier table, a microscopic imaging assembly for providing a view window for the probe 100, and a spitting assembly, the probe 100 separated from the sheath 300 is connected to the control assembly through the probe holder 110, the control assembly positions the distal end of the probe 100 to a preset position by controlling the probe holder 110, and the spitting assembly drives the reagent to enter or exit the distal opening of the probe 100.

[0048] The specific structure of the probe assembly and the operation device can be implemented in combination with the description above, and the specific structure of the centrifugal device can be implemented in combination with the prior art, which is not described here.

[0049] The technical features of the above-described embodiments can be combined arbitrarily, and in order to make the description simple, all possible combinations of the technical features in the above-described embodiments are not described, however, as long as the combination of the technical features does not exist contradictory, it should be considered that it is within the scope of the present disclosure. When the technical features in different embodiments are embodied in the same drawing, it can be considered that the drawing also discloses the combination of each embodiment involved.

[0050] The above-described embodiments only express several implementation manners of the present application, the description is more specific and detailed, but it should not be understood as a limitation on the patent application scope. It should be pointed out that for ordinary skilled in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which are within the protection scope of the present application.

Claims

1. A flexible and adaptable probe assembly, characterized in that, include: A long, hollow probe having opposing head and tail sections; A probe holder for positioning the probe, the probe holder having a filling channel communicating with the tail, through which reagents are injected into the inner cavity of the probe; The device includes a sheath, which is detachably connected to the probe holder and has a receiving cavity for accommodating the head. The sheath can position the probe and the probe holder onto a centrifuge device, and the reagent on the probe moves along the axial direction of the probe to a preset position under the centrifugal force of the centrifuge device.

2. The flexible and adaptable probe assembly according to claim 1, characterized in that, The probe assembly has a use state and a storage state; in the storage state, the probe is positioned and connected to the sheath via the probe base, and the head of the probe is suspended inside the receiving cavity; in the use state, the probe base is separated from the sheath, and the probe is connected to the control component of the operating device via the probe base, and the control component is used to move the probe relative to the stage of the operating device.

3. The flexible and adaptable probe assembly according to claim 1, characterized in that, The probe assembly also includes a base that is detachably connected to the probe holder, the base being detachably connected to the sheath, and the base having a filling hole that opens the filling channel.

4. The flexible and adaptable probe assembly according to claim 3, characterized in that, The probe assembly also includes an isolation frame disposed within the receiving cavity, the isolation frame being clamped and positioned by the probe seat and the base.

5. The flexible and adaptable probe assembly according to claim 4, characterized in that, One end of the probe holder is a mounting section that mates with the operating device or the base, and the other end is a connecting section that mates with the probe. The mounting section is narrower than the connecting section and a positioning shoulder is provided between the two. The base and the positioning shoulder clamp each other to position the isolation frame.

6. The flexible and adaptable probe assembly according to claim 5, characterized in that, The outer peripheral surface of the connecting section and the inner peripheral surface of the isolation frame are spaced apart.

7. A cellular operating system, characterized in that, Includes operating equipment and a flexibly adaptable probe assembly according to any one of claims 1 to 6; The operating device includes a stage for carrying a sample, a control component for moving the probe relative to the stage, a microscopic imaging component for providing a viewing window for the probe, and a dispensing component. When in use, the probe is connected to the control component via the probe holder. The control component positions the head of the probe to a preset position by controlling the probe holder. The dispensing component drives the reagent into or out of the opening located at the head of the probe.

8. The cell operating system according to claim 7, characterized in that, It also includes a centrifuge device, which includes a rotating stage and a positioning cavity disposed on the rotating stage. The probe is fitted into the positioning cavity through the sheath. The rotating stage is used to provide centrifugal force to drive the reagent in the probe to move in the axial direction of the probe.

9. A method for filling probe reagents, characterized in that, include: A probe assembly according to any one of claims 1 to 6 is provided, wherein a reagent is injected into the lumen of the probe via the filling channel; The probe assembly is placed in a centrifuge for centrifugation. The sheath is positioned in the centrifuge to prevent the probe from being directly exposed to the centrifuge. The centrifugal force of the centrifuge drives the reagent to move toward the head of the probe.

10. A method for injecting cells for manipulation, characterized in that, include The invention provides centrifugation equipment, operating equipment, and a probe assembly according to any one of claims 1 to 6; The probe and the probe holder are positioned on the centrifuge device by the sheath, and the reagent inside the probe moves along the axial direction of the probe to a preset position under the centrifugal force of the centrifuge device. The probe assembly is separated from the centrifuge and the probe holder and the probe are separated from the sheath. The probe is mounted onto the control component of the operating device via the probe holder. The operating device controls the distal end of the probe to be positioned at a preset position and drives the reagent to enter or exit through the distal end of the probe.

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