Animal-derived nucleic acid detection device

Abstract

The invention discloses an animal-derived nucleic acid detection device, which comprises a main body assembly, the main body assembly comprises a base, and a support frame is connected above the base; the microfluidic reaction chip comprises a lower chip body, a middle chip body and an upper chip body which are sequentially connected together from top to bottom, a plurality of lower liquid inlet channels are formed in the upper side of the lower chip body, and a reaction detection pool communicated with the lower liquid inlet channels is arranged on the lower chip body; the upper chip body is rotatably connected with a centrifugal seat with a containing cavity, the middle chip body is provided with a plurality of liquid drainage holes which are in one-to-one correspondence with the lower liquid inlet channels and can be controllably communicated with the containing cavity, and the periphery of the centrifugal seat is provided with a waste liquid drainage port, a liquid drainage port and two upper liquid inlets; a first upper liquid inlet channel and a second upper liquid inlet channel which can be respectively and controllably communicated with the accommodating cavity are formed in the upper chip body on the outer sides of the two upper liquid inlets; a waste liquid pool which can be communicated with the accommodating cavity through a waste liquid outlet is arranged on the upper chip body; according to the invention, the detection of various animal-derived substances can be realized.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microfluidic technology, and in particular to an animal-derived nucleic acid detection device. BACKGROUND

[0002] Under the background of frequent meat adulteration, animal-derived nucleic acid detection has become a key means to ensure food safety. In the prior art, nucleic acid detection is mainly achieved by traditional detection methods and detection schemes based on PCR technology. Although traditional detection methods can identify fake meat to some extent, they generally have the problems of long detection time, low sensitivity and insufficient throughput, and are difficult to meet the demand for rapid and accurate detection of large quantities of samples. Although existing microfluidic chip technology has shown high integration and high throughput potential in the field of biological detection, most microfluidic detection devices do not effectively integrate meat pretreatment modules, and additional external equipment is needed to complete the nucleic acid extraction and purification of meat samples and other pretreatment operations, resulting in fragmented detection processes and limited overall efficiency. SUMMARY

[0003] This section aims to summarize some aspects of the embodiments of the present application and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the abstract and title of the specification to avoid obscuring the purpose of this section, the abstract and the title, and such simplifications or omissions cannot be used to limit the scope of the present application.

[0004] In view of the above and / or existing problems in animal-derived nucleic acid detection, the present application is proposed.

[0005] Therefore, the purpose of the present application is to provide an animal-derived nucleic acid detection device, which integrates a pretreatment module and does not rely on external equipment, avoids fragmented detection processes, and improves overall detection efficiency.

[0006] To solve the above technical problems, the present application provides the following technical scheme: an animal-derived nucleic acid detection device, comprising, a main body assembly comprising a base, a support frame connected above the base; The microfluidic reaction chip includes a lower chip body, a middle chip body, and an upper chip body connected sequentially from top to bottom. The lower chip body has several lower liquid inlet channels on its upper side that can communicate with the outside. A mixing channel is located at the inner end of each lower liquid inlet channel on the upper side of the lower chip body. A drain channel is located at the end of the lower chip body away from the lower liquid inlet channel on the upper side of the lower chip body. A reaction detection cell is located at the end of the lower chip body away from the mixing channel on the upper side of the lower chip body. The middle chip body has several drain holes corresponding to the lower liquid inlet channels. A centrifuge with a receiving cavity is rotatably connected to the upper chip body. The centrifuge base has a waste liquid outlet, a liquid outlet, and two upper liquid inlets on its outer periphery. The upper chip body outside the two upper liquid inlets has a first upper liquid inlet channel and a second upper liquid inlet channel. The first upper liquid inlet channel and the second upper liquid inlet channel can be controllably connected to the receiving cavity. The upper chip body has a waste liquid pool. At least one control liquid outlet channel is opened at the upper end of the upper chip body. The receiving cavity can be connected to the waste liquid pool through the waste liquid outlet. One end of the control liquid outlet channel in the width direction can be connected to the receiving cavity through the liquid outlet, and the other end of the control liquid outlet channel in the width direction can be controllably connected to the corresponding liquid outlet hole.

[0007] A CCD camera for capturing reaction results is connected above the support frame; magnetic nanobeads are pre-placed inside the centrifuge holder, and an electromagnet is fixedly connected to the mounting groove on the downward-facing side of the centrifuge holder; several rotation-limiting protrusions are fixed to the lower side of the lower chip body, and an insertion groove is provided at the upward-facing end of the support frame, through which the microfluidic reaction chip is inserted into the support frame; the outer end of the lower inlet channel is connected to the outside, and several lower injection seats corresponding one-to-one with the lower inlet channel are fixedly connected to the support frame. A lower syringe is connected to the lower injection seat, and a lower injection needle is connected to the lower syringe. When the lower syringe is installed on the lower injection seat, the lower injection seat is inserted into the corresponding mixing channel. The frame is fixedly connected to two upper injection seats, corresponding to the first and second upper inlet channels respectively. An upper syringe is connected to the upper injection seat, and an upper injection needle is connected to the upper syringe. When the upper syringe is installed on the upper injection seat, the upper injection needle can be inserted precisely into the corresponding upper inlet channel. Externally, there are several first fully automatic syringes corresponding one-to-one with the lower syringes, and four second fully automatic syringes. The output end of the first fully automatic syringe is connected to a liquid outlet pipe, the end of which is connected to the corresponding lower syringe. The first fully automatic syringes provide the required primer solution to the corresponding lower syringe, while two of the second fully automatic syringes controllably inject liquid into the first upper inlet channel. Two additional fully automatic injectors controllably supply liquid to the second upper inlet channel. Taking the first upper inlet channel as an example, the two first fully automatic injectors controllably supply liquid into the first inlet channel: The two second fully automatic injectors corresponding to the first inlet channel are referred to as the first injection structure and the second injection structure, respectively. The outlet of the first injection structure is connected to a first outlet tube, and the outlet of the second injection structure is connected to a second outlet tube. One-way valves are connected to the ends of the first and second outlet tubes, respectively. A three-way valve is connected to the end of each one-way valve furthest from its corresponding outlet tube. The liquid output from the injection structure can enter the corresponding upper injector through the one-way valve. The three-way valve is furthest from the first... One end of the liquid outlet tube and the second liquid outlet tube are connected to the corresponding upper syringe; the two fully automatic syringes connected to one of the upper liquid inlets store the sample liquid to be tested and the lysis solution, respectively; the two fully automatic syringes connected to the other upper liquid inlet store the cleaning solution and the elution solution, respectively; the inner cavity of the lower syringe or the upper syringe is connected to the injection hole of the corresponding injection needle, respectively. The fully automatic syringe is existing technology, and the liquid is pumped out in a controllable manner according to the set amount by an external controller; in the initial state, one of the upper liquid inlets is connected to the receiving cavity, the waste liquid outlet is isolated from the waste liquid pool, the drain outlet is isolated from the control liquid outlet channel, and the other upper liquid inlet is isolated from the receiving cavity;In use, the second automated syringe containing the sample solution activates, opening the one-way valve. The sample solution is pumped sequentially through the one-way valve, the first outlet tube, and the first upper inlet channel into the corresponding upper inlet. After the set amount of sample solution enters the centrifuge stand, the centrifuge stand and the first upper inlet channel are isolated. The corresponding second automated syringe stops operating, causing the centrifuge stand to rotate. After the sample solution and magnetic beads mix, they come into full contact under centrifugal force. The functional groups modified on the surface of the magnetic beads specifically bind to the nucleic acids. When the centrifuge stand stops rotating, it resets, reconnecting the upper inlet and the receiving cavity. Other ports and their corresponding channels are isolated. The second automated syringe containing the lysis buffer activates, and the lysis buffer is pumped sequentially through the one-way valve, the second outlet tube, and the second... The upper inlet channel pumps the corresponding upper inlet. After the set amount of lysis buffer enters the centrifuge stand, the centrifuge stand and the second upper inlet channel are isolated. The corresponding second fully automatic injector stops operating. Under the action of the lysis buffer, the cell structure is destroyed, and nucleic acids are released into the solution and bind to the functional groups on the surface of the magnetic beads, achieving nucleic acid adsorption. After reaching the set adsorption time threshold, the electromagnet is activated, separating the magnetic beads with adsorbed nucleic acids from the lysis buffer and impurities and retaining them in the centrifuge chamber. The receiving chamber is then connected to another upper inlet, while the receiving chamber is isolated from other inlets. The second fully automatic injector activates the stored cleaning solution, and the cleaning solution is pumped out through the second inlet channel and the corresponding upper inlet into the centrifuge chamber. The magnetic beads and the cleaning solution fill the centrifuge chamber. The process involves separating the contact surfaces to remove residual impurities, improving nucleic acid purity, and providing high-quality nucleic acid for subsequent testing. The waste outlet and the receiving cavity are connected, allowing the washing solution to enter the waste pool, while the waste outlet and the receiving cavity remain isolated. A second automated syringe containing elution solution is activated, allowing the elution solution to enter the centrifuge unit through the second upper inlet channel and another upper inlet. Its components have a stronger binding affinity to nucleic acids than magnetic beads, causing nucleic acids to be released from the magnetic bead surface and enter the elution solution. After nucleic acid purification and elution, the process proceeds to the primer detection step. The control outlet channel and corresponding drain hole are connected, and then the receiving cavity and control outlet channel are connected. The purified nucleic acid solution flows through the control outlet channel and drain hole into the corresponding mixing channel, while simultaneously connecting to the mixing channel. The first fully automated syringe, storing the corresponding primers, is activated. The primer solution is pumped into the mixing channel, and the primer solution and purified nucleic acid solution are mixed and flow into the reaction detection cell through a curved drainage channel for reaction. When other primers need to be detected, the control drainage channel is connected to other corresponding drainage holes, and the above steps are repeated to complete the injection of other primers. This invention automates the entire process from nucleic acid extraction, purification, amplification to fluorescence detection, reducing manual operation and improving detection efficiency. Through the combined arrangement of multiple drainage holes, mixing channels, and corresponding reaction detection cells, it enables the detection of various target animal-derived substances, increasing detection throughput. It can be applied to the detection of animal-derived nucleic acids.

[0008] As a preferred embodiment of the animal-derived nucleic acid detection device of the present invention, wherein: the upper chip body is slidably connected to a transverse valve via a control outlet channel, the transverse valve has a control outlet hole extending through the width direction, the upper chip body outside the outlet has a first outlet channel, the side of the first outlet channel away from the outlet is always connected to one end of the control outlet hole, the upper chip body has a plurality of second outlet channels corresponding one-to-one with the outlet holes, one end of the second outlet channel is connected to the outlet hole, and the other end of the control outlet hole can be controllably connected to the other end of the second outlet channel.

[0009] As a preferred embodiment of the animal-derived nucleic acid detection device of the present invention, the centrifuge seat has four fan-shaped insertion slots arranged on it. The four fan-shaped insertion slots are respectively connected to the waste liquid outlet, the liquid outlet and the two upper liquid inlets. The centrifuge seat is connected to a flow-blocking block that can reciprocate linearly in the height direction through the insertion slots. The outer periphery of the lower part of the flow-blocking block is attached to the centrifuge seat on the inner edge of the waste liquid outlet, the liquid outlet and the two upper liquid inlets. When the bottom of the flow-blocking block abuts against the centrifuge seat, the flow-blocking block blocks the corresponding liquid outlet.

[0010] As a preferred embodiment of the animal-derived nucleic acid detection device of the present invention, wherein: a guide sleeve is fixed at the upper end of the centrifuge seat, and a plurality of transmission parts are arranged on the outer side of the guide sleeve.

[0011] As a preferred embodiment of the animal-derived nucleic acid detection device of the present invention, it further includes a centrifugal transmission assembly. The centrifugal transmission assembly includes a lifting frame movably connected to the base and capable of reciprocating motion in the height direction. A centrifugal transmission motor is fixedly connected to the upper side of the lifting frame, and a centrifugal drive shaft is connected to the centrifugal transmission motor. A support plate is fixedly connected to the lifting frame, and a mounting shell with a mounting cavity is connected to the upper side of the support plate. A rotating sleeve is fixedly connected to the lower side of the mounting shell and rotatably connected to the support plate. The rotating sleeve has a mounting hole communicating with the mounting cavity. The centrifugal drive shaft is connected to the mounting shell. Several lifting modules capable of reciprocating motion in the height direction are arranged inside the rotating sleeve, each corresponding to a transmission unit. Each lifting module includes a first lifting valve and a second lifting valve. The upper parts of the first lifting valve and the second lifting valve are fixedly connected together, and any transmission unit is located between two adjacent sets of lifting modules.

[0012] As a preferred embodiment of the animal-derived nucleic acid detection device of the present invention, wherein: a plurality of motor brackets corresponding one-to-one with the lifting module are fixedly connected inside the mounting housing, a flow-blocking drive motor is fixedly connected to the upper side of the motor bracket, a flow-blocking drive screw is rotatably connected to the flow-blocking drive motor, a lifting plate is threadedly connected to the flow-blocking drive screw, and the lifting plate is fixedly connected to the upper end of the lifting valve.

[0013] In a preferred embodiment of the animal-derived nucleic acid detection device of the present invention, in the same group of lifting modules, the first lifting valve and the second lifting valve are both provided with clearance grooves on their downward-facing sides. The first lifting valve and the second lifting valve on the upper side of the clearance grooves are respectively provided with sliding grooves. Two flow-blocking synchronization blocks are movably connected to the upper side of the flow-blocking block. A sliding part is fixed at the upper end of the flow-blocking synchronization block. The sliding part is slidably connected to the corresponding lifting valve through the corresponding sliding groove. The two flow-blocking synchronization blocks can move towards or away from each other. When the two flow-blocking synchronization blocks move towards each other, the flow-blocking synchronization block can enter the clearance groove, and the sliding part leaves the corresponding sliding groove.

[0014] As a preferred embodiment of the animal-derived nucleic acid detection device of the present invention, wherein: an avoidance motor is fixedly connected to the lifting valve, a transmission gear is rotatably connected to the avoidance motor, a plurality of transmission teeth that cooperate with the transmission gear are arranged on the inner edge of the flow blocking synchronization block, and when the sliding part enters the avoidance groove, at least one transmission tooth cooperates with the transmission gear.

[0015] As a preferred embodiment of the animal-derived nucleic acid detection device of the present invention, wherein: the inner and outer edges of the lower part of the flow blocking synchronization block are respectively fixed with a movable part that is slidably connected to the upper side of the corresponding flow blocking block, the lower end of the movable part has a movable sink groove, the upper side of the flow blocking block is fixed with a guide rail, and the movable part slides along the guide rail through the movable sink groove.

[0016] As a preferred embodiment of the animal-derived nucleic acid detection device of the present invention, wherein: at least one movable screw is rotatably connected to the lifting frame, and a movable block is threadedly connected to the movable screw and slidably connected to the lower side of the support plate; a movable protrusion is fixed to one end of the transverse valve extending from the upper side of the chip body, and an insertion groove is opened at the lower end of the movable block so as to be inserted into the movable protrusion. Attached Figure Description

[0017] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Wherein: Figure 1 This is the front view of the present invention.

[0018] Figure 2 The three-dimensional structure of the present invention Figure 1 .

[0019] Figure 3 for Figure 2 A magnified view of a portion of point A in the middle.

[0020] Figure 4The three-dimensional structure of the microfluidic chip in this invention (when the upper chip body is set to a transparent state). Figure 1 .

[0021] Figure 5 For Figure 4 A magnified view of a section at point B.

[0022] Figure 6 for Figure 4 A magnified view of a section at point C.

[0023] Figure 7 for Figure 4 A magnified view of a section at point D.

[0024] Figure 8 The three-dimensional structure of the microfluidic chip in this invention (when the upper chip body is set to a transparent state). Figure 3 .

[0025] Figure 9 for Figure 8 A magnified view of a section at point E in the middle.

[0026] Figure 10 The three-dimensional structure of the present invention Figure 2 .

[0027] Figure 11 for Figure 10 A magnified view of a section at point F.

[0028] Figure 12 for Figure 10 A magnified view of a section at point G.

[0029] Figure 13 The three-dimensional structure of the present invention Figure 3 .

[0030] Figure 14 for Figure 13 A magnified view of a section at point H.

[0031] Figure 15 This is a three-dimensional structural diagram of the microfluidic chip in this invention (when both the chip body and the centrifuge base are set to a transparent state).

[0032] Figure 16 for Figure 15 A magnified view of a section at point I.

[0033] Figure 17 This is a three-dimensional structural diagram of the lower chip body in a microfluidic chip.

[0034] Figure 18 A three-dimensional structural diagram showing the rotating sleeve fixed to the lower side of the mounting housing.

[0035] Figure 19 This is a 3D structural diagram of the lifting module.

[0036] Figure 20 A three-dimensional structural diagram of the microfluidic chip with the upper chip body and the middle chip body set to a transparent state.

[0037] In the diagram, 100 is the main component, 101 is the base, 102 is the support frame, 103 is the fixing frame, 103a is the guide plate, 200 is the centrifugal transmission assembly, 201 is the centrifugal transmission motor, 202 is the lifting frame, 202a is the lifting rack, and 203 is the mounting housing. 204 Lifting Module, 204a First Lifting Valve, 204a-1 Sliding Sink, 204a-2 Clearance Slot, 204b Second Lifting Valve, 205 Rotating Sleeve, 205a Guide Bracket, 205a-1 Sliding Slot, 205b Guide Shaft, 206 Transmission Gear, 207 Clearance Motor, 208 Centrifugal Drive Shaft, 209 Moving Screw, 210 Lifting Motor, 211 Lifting Gear, 212 Lifting Plate, 213 Flow-Blocking Transmission Motor, 214 Motor Bracket, 215 Flow-Blocking Transmission Screw, 216 Moving Block, 300 Microfluidic Reaction Chip, 301 Flow-Blocking Block, 301a Guide Rail, 301b Blocking Part, 302 Flow-Blocking Synchronizing Block, 302a Sliding Part, 302a-1 Transmission Gear, 302b Moving Part, 302b-1 Positioning Hole, 303 Upper Chip Body, 303a Second Lifting Valve 303b Second liquid outlet channel, 303c Control liquid outlet channel, 303d Waste liquid tank, 303e First upper liquid inlet channel, 303f Second upper liquid inlet channel, 303g Upper connecting hole, 303h Drain hole, 304 Intermediate chip body, 304a Drain hole, 304b Connecting channel, 304c Intermediate connecting hole, 305 Lower chip body, 305a Lower liquid inlet channel, 305b Rotation limiting protrusion, 305c Mixing channel, 305d Reaction detection cell, 306 Centrifuge seat, 306a Drain port, 306b Transmission part, 306c Guide sleeve, 306d Waste liquid outlet, 306e Upper liquid inlet, 307 Horizontal valve, 307a Moving protrusion, 307b Control liquid outlet hole, 400 Lower injection seat, 401 Insertion hole, 500 Lower syringe, 600 Lower injection needle. Detailed Implementation

[0038] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0039] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0040] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that is mutually exclusive with other embodiments.

[0041] Example 1 Reference Figure 1 , Figure 2 and Figures 6-10 This embodiment provides an animal-derived nucleic acid detection device that can detect different animal-derived substances.

[0042] An animal-derived nucleic acid detection device includes a main component 100, which includes a base 101. A support frame 102 is connected above the base 101, and a microfluidic reaction chip 300 is inserted into the support frame 102.

[0043] Specifically, the microfluidic reaction chip 300 includes a lower chip body 305, an intermediate chip body 304, and an upper chip body 303 connected sequentially from top to bottom. The lower chip body 305 has several lower liquid inlet channels 305a that can communicate with the outside. A curved mixing channel 305c is formed on the upper side of the lower chip body 305 at the inner end of the lower liquid inlet channel 305a. A drain channel is formed on the upper side of the lower chip body 305 at the end of the mixing channel 305c away from the lower liquid inlet channel 305a. A reaction detection cell 305d is provided on the upper side of the lower chip body 305 at the end of the drain channel away from the mixing channel 305c. The intermediate chip body 304 has several drain holes 304a corresponding one-to-one with the lower liquid inlet channels 305a. A centrifuge seat 306 with a receiving cavity is rotatably connected to the upper chip body 303. The centrifuge seat 306 has a waste liquid outlet 306d, a drain outlet 306a, and two... There is one upper liquid inlet 306e. The upper chip body 303 outside the two upper liquid inlets 306e has a first upper liquid inlet channel 303e and a second upper liquid inlet channel 303f. The first upper liquid inlet channel 303e and the second upper liquid inlet channel 303f can be controllably connected to the receiving cavity. The upper chip body 303 has a waste liquid pool 303d. The upper end of the upper chip body 303 has at least one control liquid outlet channel 303c. In this embodiment, the upper end of the upper chip body 303 has two control liquid outlet channels 303c. The two control liquid outlet channels 303c are symmetrically arranged about the center of the upper chip body 303. The receiving cavity can be connected to the waste liquid pool 303d through the waste liquid outlet 306d. One end of the control liquid outlet channel 303c in the width direction can be connected to the receiving cavity through the liquid outlet 306a. The other end of the control liquid outlet channel 303c in the width direction can be controllably connected to the corresponding liquid outlet hole 304a.

[0044] A CCD camera for capturing reaction results is also installed above the support frame 102; magnetic nanobeads are pre-placed inside the centrifuge seat 306, and a mounting groove is provided on the downward-facing side of the centrifuge seat 306, in which an electromagnet is fixedly connected (this is prior art); several rotation-limiting protrusions 305b are fixedly attached to the lower side of the lower chip body 305, and an insertion groove is provided on the upward-facing end of the support frame 102, through which the microfluidic reaction chip 300 is inserted into the support frame 102; the outer end of the lower liquid inlet channel 305a is connected to the outside, and several corresponding lower liquid inlet channels 305a are fixedly connected to the support frame 102. The lower injection seat 400 has a lower syringe 500 connected to it, and a lower injection needle 600 connected to the lower syringe 500. A connecting block is fixed to the upper end of the lower syringe 500, which is detachably connected to the lower injection seat 400. The connecting block is fixed to the lower injection seat 400 using fasteners such as screws. The lower injection seat 400 has a insertion hole 401 for easy passage of the lower syringe 500 and the lower injection needle 600. When installed on the lower injection seat 400, the lower injection needle 600 is inserted into the corresponding lower inlet channel 305a. Two sub-channels are fixedly connected to the support frame 102. The upper injection seat corresponds to the first upper inlet channel 303e and the second upper inlet channel 303f. An upper syringe is connected to the upper injection seat, and an upper injection needle is connected to the upper syringe. When the upper syringe is installed on the upper injection seat, the upper injection needle can be inserted precisely into the corresponding upper inlet channel (the upper syringe is detachably connected to the upper injection seat, and its connection method is the same as that between the lower syringe 500 and the lower injection seat 400, which will not be described further here). Externally, there are several first fully automatic syringes corresponding one-to-one with the lower syringe 500, and four second fully automatic syringes. The output end of the first fully automatic syringe is connected to... The liquid outlet pipe is connected to the corresponding lower syringe 500 at its end. The required primer liquid is provided to the corresponding lower syringe 500 through the first fully automatic syringe. Two second fully automatic syringes controllably inject liquid into the first upper liquid inlet channel 303e, and two other second fully automatic syringes controllably inject liquid into the second upper liquid inlet channel 303f. (The connection between the fully automatic syringe and the upper or lower syringe via the pipe is prior art. The connection between the one-way valve and the corresponding pipe is also prior art. It is not an improvement point of this application. The absence of a connection diagram does not affect the understanding of the above description by those skilled in the art.)Taking the first upper inlet channel 303e as an example, the following describes how the two first fully automatic injectors controllably inject liquid into the first inlet channel: The two second fully automatic injectors corresponding to the first inlet channel are referred to as the first injection structure and the second injection structure, respectively. The outlet of the first injection structure is connected to a first outlet tube, and the outlet of the second injection structure is connected to a second outlet tube. The ends of the first and second outlet tubes are respectively connected to one-way valves. The ends of the two one-way valves away from their corresponding outlet tubes are connected to a three-way tube. The liquid output from the injection structure can enter the corresponding upper injector through the one-way valves. The end of the three-way tube away from the first and second outlet tubes is connected to the corresponding upper injector. Two fully automated injectors connected to one of the upper inlet ports 306e store the sample solution to be tested and the lysis buffer, respectively; two fully automated injectors connected to the other upper inlet port 306e store the cleaning solution and the elution solution, respectively; the inner cavity of the lower injector 500 or the upper injector is connected to the injection port of the corresponding injection needle, respectively. The fully automated injectors are existing technology, and are controlled by an external controller to pump out liquid controllably according to a set amount; in the initial state, one of the upper inlet ports 306e is connected to the receiving cavity, the waste liquid outlet 306d is isolated from the waste liquid pool 303d, the drain port 306a is isolated from the control outlet channel 303c, and the other upper inlet port... The inlet 306e and the receiving cavity are isolated. During use, the second fully automated injector containing the sample solution is activated, the one-way valve opens, and the sample solution is pumped sequentially through the one-way valve, the first outlet tube, and the first upper inlet channel 303e into the corresponding upper inlet 306e. After the set amount of sample solution enters the centrifuge seat 306, the centrifuge seat 306 and the first upper inlet channel 303e are isolated. The corresponding second fully automated injector stops operating, causing the centrifuge seat 306 to rotate. After the sample solution and magnetic beads mix, they come into full contact under centrifugal force. The functional groups modified on the surface of the magnetic beads specifically bind to the nucleic acid. When the centrifuge seat 306 stops rotating, it resets; thus, the upper inlet 306e and the receiving cavity are reconnected. The second fully automatic injector, which stores lysis buffer, is activated. The lysis buffer is pumped sequentially through a one-way valve, a second outlet pipe, and a second upper inlet channel 303f into the corresponding upper inlet 306e. After the set amount of lysis buffer enters the centrifuge seat 306, the centrifuge seat 306 and the second upper inlet channel 303f are isolated, and the corresponding second fully automatic injector stops operating. Under the action of the lysis buffer, the cell structure is destroyed, and nucleic acids are released into the solution and bind to the functional groups on the surface of the magnetic beads to achieve nucleic acid adsorption. After the set adsorption time threshold is reached, the electromagnet is activated to separate the magnetic beads adsorbed with nucleic acids from the lysis buffer and impurities and retain them in the centrifuge chamber.The receiving chamber is connected to another upper inlet 306e, while the receiving chamber is isolated from other inlets. The second automated syringe, storing the washing solution, is activated, pumping the washing solution through the second inlet channel and the corresponding upper inlet 306e into the centrifuge chamber. The magnetic beads fully contact the washing solution, removing residual impurities and improving nucleic acid purity, providing high-quality nucleic acid for subsequent testing. The waste outlet 306d is then connected to the receiving chamber, allowing the washing solution to enter the waste tank 303d. The waste outlet 306d is isolated from the receiving chamber. The second automated syringe, storing the elution solution, is activated. The eluent enters the centrifuge seat 306 through the second upper inlet channel 303f and another upper inlet 306e. Its components have a stronger binding affinity to nucleic acids than the magnetic beads, causing the nucleic acids to be released from the magnetic bead surface and enter the eluent. After nucleic acid purification and elution, the primer detection step begins. The control outlet channel 303c and the corresponding drain hole 304a are connected, and then the receiving cavity and the control outlet channel 303c are connected. The purified nucleic acid solution flows through the control outlet channel 303c and the drain hole 304a into the corresponding mixing channel 305c, simultaneously mixing with the... The first fully automated syringe, connected to the mixing channel 305c and storing the corresponding animal-derived primer solution, is activated. The first fully automated syringe pumps the primer solution into the mixing channel 305c. The primer solution and purified nucleic acid solution are mixed and flow into the reaction detection cell 305d through a curved drainage channel for reaction. When other primers need to be detected, the control outlet channel 303c is connected to other corresponding drainage holes 304a, and the above steps are repeated to complete the injection of other primers. During detection, the reaction detection cell 305d can be photographed using a CCD camera. This photographing process enables real-time detection and analysis of several target animal-derived substances. By converting the captured fluorescence images into digital signals, the fluorescence intensity value of each detection area is calculated, thereby determining whether the sample contains the animal-derived substance corresponding to that detection area. This invention achieves full automation of the entire process from nucleic acid extraction, purification, amplification to fluorescence detection, reducing manual operation and improving detection efficiency. Through the combined arrangement of multiple drainage holes 304a, the mixing channel 305c, and the corresponding reaction detection cell 305d, the detection of multiple target animal-derived substances can be achieved, increasing detection throughput. ;

[0045] The aforementioned magnetic beads, cleaning solution, lysis solution, eluent, and other solutions are all existing technologies and are not improvements in this application. Therefore, their specific components and preparation methods need not be described in detail here.

[0046] Example 2: Refer to Figures 1-5 This embodiment provides an animal-derived nucleic acid detection device. The difference between this embodiment and Embodiment 1 is that it can further realize the rotation of the centrifuge seat 306 and the opening and closing of each liquid outlet and the receiving cavity.

[0047] Specifically, the centrifuge seat 306 has four fan-shaped insertion slots arranged on it. The four fan-shaped insertion slots are respectively connected to the waste liquid discharge port 306d, the liquid discharge port 306a and the two upper liquid inlets 306e. The centrifuge seat 306 is connected to a flow-blocking block 301 that can reciprocate linearly in the height direction through the insertion slots. The outer periphery of the lower part of the four flow-blocking blocks 301 is respectively attached to the inner edge of the centrifuge seat 306 of the waste liquid discharge port 306d, the liquid discharge port 306a and the two upper liquid inlets 306e. A blocking part 301b is fixed on the lower side of the flow-blocking block 301. When the bottom of the blocking part 301b abuts against the centrifuge seat 306, the blocking part 301b blocks the corresponding liquid outlet.

[0048] In the initial state, the flow-blocking block 301 corresponding to one of the upper liquid inlets 306e is above it, that is, the receiving cavity and the first upper liquid inlet channel 303e are connected. The bottoms of the other three flow-blocking blocks 301 are in contact with the centrifuge base 306. The other upper liquid inlet 306e, the waste liquid outlet and the drain outlet 306a are blocked by the corresponding flow-blocking blocks 301 respectively. The receiving cavity and the second upper liquid inlet channel 303f, the waste liquid pool 303d and the control outlet channel 303c are isolated. When it is necessary to isolate one of the upper liquid inlets 306e from the receiving cavity, the corresponding flow-blocking block 301 is lowered, so that the flow-blocking block 301 is in contact with the centrifuge base 306. The flow-blocking block 301 blocks the upper liquid inlet 306e. The steps to connect the other liquid outlets with the receiving cavity are the reverse of the above, and will not be repeated here.

[0049] Specifically, it also includes a centrifugal transmission assembly 200, which includes a lifting frame 202 movably connected to the base 101 and capable of reciprocating motion in the height direction. A centrifugal transmission motor 201 is fixedly connected to the upper side of the lifting frame 202, and a centrifugal drive shaft 208 is connected to the centrifugal transmission motor 201. A support plate is fixedly connected to the lifting frame 202, and a mounting housing 203 with a mounting cavity is rotatably connected to the upper side of the support plate. A rotating sleeve 205 is fixedly fixed to the lower side of the mounting housing 203 and rotatably connected to the support plate. The rotating sleeve 205 has a mounting hole communicating with the mounting cavity. The centrifugal drive shaft 208 and the mounting housing 203 are fixedly connected. Several lifting modules 204, each corresponding to the transmission part 306b, are arranged inside the mounting housing 203 and the rotating sleeve 205 and capable of reciprocating motion in the height direction. 4 includes a first lifting valve 204a and a second lifting valve 204b slidably connected within a rotating sleeve 205. A guide bracket 205a is fixed at the center of the rotating sleeve 205. Four sliding grooves 205a-1 corresponding one-to-one with the lifting modules 204 are arranged on the guide bracket 205a. The upper parts of the first lifting valve 204a and the second lifting valve 204b are fixedly connected together. The first lifting valve 204a and the second lifting valve 204b connected together slide up and down along the corresponding sliding grooves 205a-1. A guide shaft 205b is fixed at the lower end of the center of the guide bracket 205a. A guide sleeve 306c is fixed at the upper end of the centrifugal seat 306. The guide shaft 205b is rotatably connected within the guide sleeve 306c. Several transmission parts 306b are arranged on the outer side of the guide sleeve 306c. Any transmission part 306b is located between two adjacent sets of lifting modules 204.

[0050] When it is necessary to fully mix the sample liquid and the magnetic beads, the centrifugal drive motor 201 is activated, the centrifugal drive shaft 208 rotates, the centrifugal drive shaft 208 drives the rotating sleeve 205 and the four lifting modules 204 to rotate via the mounting housing 203, and the four lifting modules 204 drive the centrifugal seat 306 to rotate via the transmission part 306b. When the mixing time threshold is reached, the centrifugal drive motor 201 stops operating.

[0051] Specifically, the mounting housing 203 has several motor brackets 214 corresponding to the lifting modules 204, which are fixedly connected inside. A flow-blocking drive motor 213 is fixedly connected to the upper side of each motor bracket 214. A flow-blocking drive screw 215, rotatably connected to the flow-blocking drive motor 213 and mounted on the motor bracket 214, is connected to the flow-blocking drive screw 215. A lifting plate 212 is threaded onto the flow-blocking drive screw 215. The lifting plate 212 is fixedly connected to the upper end of the corresponding first lifting valve 204a and second lifting valve 204b. In the same group of lifting modules 204, a clearance groove 204a-2 is provided on the downward-facing side of both the first lifting valve 204a and the second lifting valve 204b. The first lift valve 204a and the second lift valve 204b on the upper side of 204a-2 have sliding grooves 204a-1 respectively. The front and rear sides of the upper part of the flow-blocking block 301 are straight. Two flow-blocking synchronizing blocks 302 are slidably connected to the upper side of the flow-blocking block 301. The upper end of the flow-blocking synchronizing block 302 is fixed with a sliding part 302a. The two sliding parts 302a are slidably connected to the first lift valve 204a and the second lift valve 204b respectively through the corresponding sliding grooves 204a-1. The two flow-blocking synchronizing blocks 302 can move towards or away from each other. When the two flow-blocking synchronizing blocks 302 move towards each other, the flow-blocking synchronizing blocks 302 can enter the clearance groove 204a-2 and slide. Part 302a leaves the corresponding sliding trough 204a-1; both the first lifting valve 204a and the second lifting valve 204b are fixedly connected to a clearance motor 207, and the clearance motor 207 is connected to a transmission gear 206 rotatably connected to the corresponding lifting valve; the inner edge of the flow-blocking synchronization block 302 has a number of transmission teeth 302a-1 that cooperate with the transmission gear 206; when the sliding part 302a enters the clearance trough 204a-2, at least one transmission tooth 302a-1 cooperates with the transmission gear 206; the inner and outer edges of the lower part of the flow-blocking synchronization block 302 are respectively fixed with a moving part 302b that is slidably connected to the upper side of the corresponding flow-blocking block, and the moving part 302b... The lower end has a movable recess, and a linear guide rail 301a is fixed on the upper side of the intercepting block. A connecting groove is opened on the guide rail 301a. The moving part 302b slides along the guide rail 301a via the movable recess. The moving part 302b is connected to the intercepting block in the height direction via the guide rail 301a. The moving part 302b has a positioning hole 302b-1, and the intercepting block has a positioning countersunk hole that is coaxial with the positioning hole 302b-1. The positioning countersunk hole is located in the area below the clearance groove 204a-2. When the positioning hole 302b-1 and the positioning countersunk hole are coaxial, the transmission gear 206 above can cooperate with the transmission gear 302a-1 on the sliding part 302a.

[0052] When the microfluidic reaction chip 300 is first installed on the support frame 102, the two sliding parts 302a between the first lifting valve 204a and the second lifting valve 204b in the same set of lifting modules 204 are respectively in the clearance groove 204a-2, and the lifting module 204 is above the flow-blocking synchronization block 302. Adjust the position of the two flow-blocking synchronization blocks 302 so that the positioning hole 302b-1 and the corresponding positioning countersunk hole are coaxial. Use positioning pins to insert into the positioning hole 302b-1 and the positioning countersunk hole to position the flow-blocking synchronization block 302 in the circumferential direction, prevent the flow-blocking synchronization block 302 from moving circumferentially when the lifting module 204 is lowered, and improve the transmission. The smoothness of the engagement between the moving gear 206 and the sliding part 302a when the moving gear 206 moves downward; when the lifting module 204 descends to the height at which the sliding groove 204a-1 is aligned with the sliding part 302a, the positioning pin is pulled out, the lifting module 204 stops descending, the avoidance motor 207 is activated, the transmission gear 206 rotates, the transmission gear 206 drives the sliding part 302a to move, the sliding part 302a moves to the set position in the sliding groove 204a-1, the avoidance motor 207 stops operating, at this time, when the lifting module 204 moves in the height direction, the flow blocking synchronizing block 302 and the flow blocking block 301 rise and fall synchronously with the corresponding lifting module 204.

[0053] After the test is completed, the flow-blocking synchronization block 302 needs to enter the clearance groove 204a-2. The clearance motor 207 is controlled to move, so that the corresponding two flow-blocking synchronization blocks 302 move towards each other. When the corresponding two flow-blocking synchronization blocks 302 move into the clearance groove 204a-2, that is, when the moving part 302b leaves the sliding sink 204a-1 to a suitable position, the clearance motor 207 stops moving, and the flow-blocking synchronization block 302 disengages from the lifting module 204. At this time, the lifting module 204 is controlled to rise, so that the lifting module 204 is completely disengaged from the microfluidic reaction chip 300. The microfluidic reaction chip 300 can be removed from the support frame 102 for easy replacement with a new microfluidic reaction chip 300.

[0054] Example 3: Reference Figures 1-5 This embodiment provides an animal-derived nucleic acid detection device. The difference between this embodiment and Embodiment 1 is that it can further controllably realize the opening and closing of the first liquid outlet channel 303a and the second liquid outlet channel 303b.

[0055] Specifically, a fixing frame 103 is fixed to the upper side of the base 101 at one end of the support frame 102 in the front-rear direction. A guide plate 103a for guiding the lifting frame 202 is fixed to the upper side of the fixing frame 103. The lifting frame 202 is slidably connected to the guide plate 103a. Two lifting motors 210 are fixedly connected to the rear side of the fixing frame 103. Lifting gears 211 are connected to the lifting motors 210. Lifting racks 202a that mesh with the corresponding lifting gears 211 are fixed to the left and right ends of the lifting frame 202. The upper chip body 303 is slidably connected to a transverse valve 307 via a controlled liquid outlet channel 303c. A movable protrusion 307a is fixed to one end of the transverse valve 307 extending from the upper side of the upper chip body 303. At least one horizontally arranged movable lead screw 209 is rotatably connected to the lifting frame 202. In this embodiment, two horizontally arranged movable lead screws 209 are rotatably connected to the lifting frame 202. Two movable motors are fixedly connected to one side of the lifting frame 202 in the front-rear direction. The two movable motors are respectively connected to the two movable lead screws 209. The moving lead screw 209 is threadedly connected to a sliding block 216 slidably connected to the lower side of the support plate. The downward-facing end of the moving block 216 has an insertion groove that can be inserted into the moving protrusion 307a. The transverse valve 307 has a control outlet hole 307b that extends through the width direction. The upper chip body 303 outside the drain port 306a has a first outlet channel 303a. The side of the first outlet channel 303a away from the drain port 306a is connected to one end of each of the two control outlet channels 303c in the width direction. The main body 303 has several second liquid outlet channels 303b that correspond one-to-one with the drain holes 304a. Each control liquid outlet channel 303c has four second liquid outlet channels 303b on one end of the upper chip main body 303 in the width direction. Alternatively, the corresponding number of second liquid outlet channels 303b and drain holes 304a can be set according to actual needs. One end of the second liquid outlet channel 303b is connected to the drain hole 304a, and the other end of the control liquid outlet hole 307b can be controllably connected to the other end of the second liquid outlet channel 303b.

[0056] Before operation, the moving motor is activated, and the moving screw 209 rotates, driving the moving block 216 to move. When the moving block 216 moves to align the insertion groove with the moving protrusion 307a, the moving motor stops. The lifting motor 210 is then activated, and the lifting gear 211 rotates, driving the lifting frame 202 to move in the height direction. The direction of the lifting motor 210 is controlled to lower the lifting frame 202. When the moving block 216 is inserted into the moving protrusion 307a via the insertion groove, the moving motor stops. When it is necessary to adjust the connection between the control outlet 307b and the required second outlet channel 303b, the corresponding moving motor is activated, and the corresponding moving block 216 moves. The moving block 216 drives the transverse valve 307 to move via the moving protrusion 307a. The transverse valve 307 slides along the length of the control outlet channel 303c. When the control outlet 307b and the required second outlet channel 303b are connected, the moving motor stops.

[0057] As can be seen from the above, this embodiment allows the purified nucleic acid solution to be controlled and introduced into the required second outlet channel 303b.

[0058] Example 4: Referring to the figure, the difference from Example 3 is that it can accelerate the flow of waste liquid into waste liquid pool 303d and the flow of nucleic acid liquid into reaction detection pool 305d.

[0059] Specifically, a drainage hole 303h communicating with the waste liquid pool 303d is opened on the upper chip body 303 at the position of the waste liquid pool 303d. A connection channel 304b communicating with each reaction detection pool 305d is opened on the lower side of the middle chip body 304. An intermediate connecting hole 304c is opened on the middle chip body 304 above the connection channel 304b between two adjacent reaction detection pools 305d. An upper connecting hole 303g communicating with the intermediate connecting hole 304c is opened on the upper chip body 303.

[0060] In practice, a peristaltic pump is connected to the upper chip body 303 via the drainage hole 303h. When waste liquid needs to flow into the waste liquid pool 303d, the peristaltic pump works to make the waste liquid flow in the direction of the waste liquid pool 303d. Another peristaltic pump is connected to the upper chip body 303 via the upper connecting hole 303g. When the nucleic acid solution flows out of the centrifuge seat 306 and is connected to the second outlet channel 303b and the first outlet channel 303a, the peristaltic pump works to promote the flow of the nucleic acid solution in the direction of the corresponding reaction detection pool 305d.

[0061] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. An animal-derived nucleic acid detection device, characterized in that: It includes, The main components include a base, with a support frame connected to the top of the base; The microfluidic reaction chip includes a lower chip body, a middle chip body, and an upper chip body connected sequentially from top to bottom. The lower chip body has several lower liquid inlet channels on its upper side that can communicate with the outside. A mixing channel is located at the inner end of each lower liquid inlet channel on the upper side of the lower chip body. A drain channel is located at the end of the lower chip body away from the lower liquid inlet channel on the upper side of the lower chip body. A reaction detection cell is located at the end of the lower chip body away from the mixing channel on the upper side of the lower chip body. The middle chip body has several drain holes corresponding to the lower liquid inlet channels. A centrifuge with a receiving cavity is rotatably connected to the upper chip body. The centrifuge base has a waste liquid outlet, a liquid outlet, and two upper liquid inlets on its outer periphery. The upper chip body outside the two upper liquid inlets has a first upper liquid inlet channel and a second upper liquid inlet channel. The first upper liquid inlet channel and the second upper liquid inlet channel can be controllably connected to the receiving cavity. The upper chip body has a waste liquid pool. At least one control liquid outlet channel is opened at the upper end of the upper chip body. The receiving cavity can be connected to the waste liquid pool through the waste liquid outlet. One end of the control liquid outlet channel in the width direction can be connected to the receiving cavity through the liquid outlet, and the other end of the control liquid outlet channel in the width direction can be controllably connected to the corresponding liquid outlet hole.

2. The animal-derived nucleic acid detection device as described in claim 1, characterized in that: The upper chip body is slidably connected to a transverse valve via a control liquid outlet channel. The transverse valve has a control liquid outlet hole that extends through the width direction. The upper chip body outside the drain port has a first liquid outlet channel. The side of the first liquid outlet channel away from the drain port is always connected to one end of the control liquid outlet hole. The upper chip body has several second liquid outlet channels that correspond one-to-one with the drain holes. One end of the second liquid outlet channel is connected to the drain hole, and the other end of the control liquid outlet hole can be controllably connected to the other end of the second liquid outlet channel.

3. The animal-derived nucleic acid detection device as described in claim 1, characterized in that: The centrifuge base has four fan-shaped insertion slots arranged on it. The four fan-shaped insertion slots are connected to the waste liquid discharge port, the liquid discharge port and the two upper liquid inlets, respectively. The centrifuge base is connected to a flow-blocking block that can reciprocate linearly in the height direction through the insertion slots. The outer periphery of the lower part of the flow-blocking block is attached to the centrifuge base on the inner edge of the waste liquid discharge port, the liquid discharge port and the two upper liquid inlets. When the bottom of the flow-blocking block touches the centrifuge base, the flow-blocking block blocks the corresponding liquid outlet.

4. The animal-derived nucleic acid detection device as described in claim 3, characterized in that: The upper end of the centrifugal seat is fixed with a guide sleeve, and several transmission parts are arranged on the outside of the guide sleeve.

5. The animal-derived nucleic acid detection device as described in claim 4, characterized in that: It also includes a centrifugal transmission assembly, which includes a lifting frame movably connected to the base and capable of reciprocating motion in the height direction. A centrifugal transmission motor is fixedly connected to the upper side of the lifting frame, and a centrifugal drive shaft is connected to the centrifugal transmission motor. A support plate is fixedly connected to the lifting frame, and a mounting housing with a mounting cavity is connected to the upper side of the support plate. A rotating sleeve rotatably connected to the support plate is fixed to the lower side of the mounting housing. The rotating sleeve has a mounting hole communicating with the mounting cavity. The centrifugal drive shaft is connected to the mounting housing. Several lifting modules capable of reciprocating motion in the height direction are arranged inside the rotating sleeve, each corresponding to a transmission unit. Each lifting module includes a first lifting valve and a second lifting valve. The upper parts of the first lifting valve and the second lifting valve are fixedly connected together. Any transmission unit is located between two adjacent sets of lifting modules.

6. The animal-derived nucleic acid detection device as described in claim 5, characterized in that: The mounting housing has several motor brackets that correspond one-to-one with the lifting module. A flow-blocking drive motor is fixedly connected to the upper side of the motor bracket. A flow-blocking drive screw is rotatably connected to the flow-blocking drive motor and threadedly connected to the flow-blocking drive screw. The lifting plate is fixedly connected to the upper end of the lifting valve.

7. The animal-derived nucleic acid detection device as described in claim 5, characterized in that: In the same set of lifting modules, both the first lifting valve and the second lifting valve have clearance grooves on their downward-facing sides. The first lifting valve and the second lifting valve on the upper side of the clearance grooves have sliding grooves respectively. Two flow-blocking synchronizing blocks are movably connected to the upper side of the flow-blocking block. The upper end of the flow-blocking synchronizing block is fixed with a sliding part. The sliding part is slidably connected to the corresponding lifting valve through the corresponding sliding groove. The two flow-blocking synchronizing blocks can move towards or away from each other. When the two flow-blocking synchronizing blocks move towards each other, the flow-blocking synchronizing block can enter the clearance groove, and the sliding part leaves the corresponding sliding groove.

8. The animal-derived nucleic acid detection device as described in claim 7, characterized in that: An avoidance motor is fixedly connected to the lifting valve, and a transmission gear is rotatably connected to the lifting valve on the avoidance motor. Several transmission teeth that cooperate with the transmission gear are arranged on the inner edge of the flow blocking synchronizing block. When the sliding part enters the avoidance groove, at least one transmission tooth cooperates with the transmission gear.

9. The animal-derived nucleic acid detection device as described in claim 7, characterized in that: The inner and outer edges of the lower part of the flow-blocking synchronization block are respectively fixed with a movable part that is slidably connected to the upper side of the corresponding flow-blocking block. The lower end of the movable part has a movable groove, and a guide rail is fixed on the upper side of the flow-blocking block. The movable part slides along the guide rail through the movable groove.

10. The animal-derived nucleic acid detection device according to any one of claims 1 to 9, characterized in that: At least one movable lead screw is rotatably connected to the lifting frame. A movable block is threadedly connected to the movable lead screw and slidably connected to the lower side of the support plate. A movable protrusion is fixed to one end of the transverse valve extending from the upper side of the chip body. An insertion groove is opened at the lower end of the movable block so that it can be inserted into the movable protrusion.

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