Sensitive skin care composition with soothing and repairing effects and application thereof

Abstract

The invention relates to a sensitive skin care composition with soothing and repairing effects and application of the sensitive skin care composition, the sensitive skin care composition is prepared from 4-tert-butyl cyclohexanol, kadsura longepedunculata extract and tephrosia rubescens seed extract, and the mass ratio of the 4-tert-butyl cyclohexanol to the kadsura longepedunculata extract to the tephrosia rubescens seed extract is 1: (0.01-0.1): (0.01-0.1); wherein the kadsura longepedunculata extract and the tephrosia rubescens seed extract are both obtained by adopting an enzymolysis-fermentation process, the composition disclosed by the invention has a good soothing and repairing effect, the active ingredient combination shows good compatibility and stability in a formula system, a solubilizer and an emulsifying agent do not need to be additionally added, and the composition is suitable for more mild dosage forms and has a good application prospect. The requirements of moderate and severe sensitive skin are better met.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a skincare composition for sensitive skin with soothing and repairing effects and its application. Background Technology

[0002] In today's society, people generally experience significant work and study pressure. Prolonged exposure to this high-pressure state, coupled with constant changes and stimuli from the external climate, can lead to abnormal secretion of the skin's sebaceous and sweat glands. As we age, our skin naturally deteriorates; under the combined effects of stress and environmental factors, sebum secretion decreases, and the skin's ability to retain moisture declines, resulting in dryness. The stratum corneum of the epidermis gradually thickens, causing keratinization, cracking, and a rough skin surface, among other problems. In recent years, with the continuous improvement of people's awareness of beauty, effective moisturizing and repairing has become one of the current research hotspots in the skincare field.

[0003] CN117357460B discloses a soothing and protective composition including 4-tert-butylcyclohexanol, Schisandra chinensis extract, and Edamame seed extract. 4-tert-butylcyclohexanol is a typical fat-soluble component (logP≈3.5, almost insoluble in water, readily soluble in ethanol and oils). Schisandra chinensis extract contains residual polysaccharides and proteins with strong hydrophilicity, and Edamame seed extract also contains hydrophilic components. Therefore, there is a polarity conflict between 4-tert-butylcyclohexanol, Schisandra chinensis extract, and Edamame seed extract. In order to maintain compatibility, CN117357460B adds a large amount of oily components (squalane, palm oil) and polyols (1,2-pentanediol), resulting in inherent limitations of the formulation: the oily components increase the stickiness of the formulation, making it only suitable for oily bases such as lotions and creams, and unable to develop alcohol-free and refreshing formulations (such as toners and serums) that meet the needs of sensitive skin.

[0004] CN116712342B also discloses extracts including 4-tert-butylcyclohexanol, Schisandra chinensis extract, and Edamame seed extract. It also faces the problem of poor compatibility of 4-tert-butylcyclohexanol, Schisandra chinensis extract, and Edamame seed extract. CN116712342B solves this problem by using 4-tert-butylcyclohexanol as the shell material, while Schisandra chinensis extract and Edamame seed extract are used as the encapsulated contents. Because it relies on the vesicle structure, the formula needs to contain a large amount of nonionic surfactants and cholesterol, which can only be used in oily bases such as lotions and creams. In addition, the high proportion of the capsule material limits the space for adding active ingredients. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and provide a skin care composition for sensitive skin with soothing and repairing effects and its application. The two plant fermentation extracts and 4-tert-butylcyclohexanol have good compatibility and do not require a large amount of emulsifiers or microcapsule preparation. They are easier to apply to various dosage forms such as water-based, alcohol-free, and refreshing types (such as toners and serums), and the formula texture can be adjusted according to the type of sensitive skin (dry sensitive skin, oily sensitive skin).

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides a skin care composition for sensitive skin with soothing and repairing effects, comprising 4-tert-butylcyclohexanol, Schisandra chinensis extract and Edamame seed extract, wherein the mass ratio of 4-tert-butylcyclohexanol, Schisandra chinensis extract and Edamame seed extract is 1:(0.01-0.1):(0.01-0.1). The preparation method of the Schisandra chinensis extract includes the following steps: S1. Crush Schisandra chinensis, add 5-6 times its weight of water, adjust the pH to 5.5-6.5, add the first complex enzyme for enzymatic hydrolysis, inactivate the enzyme, cool to room temperature, and obtain the enzymatic hydrolysate; wherein, the first complex enzyme includes cellulase, pectinase and papain. S2. Sterilize the enzymatic hydrolysate obtained in step S1, then inoculate the enzymatic hydrolysate with the first compound fermentation liquid for fermentation, sterilize, filter, and obtain fermentation liquid and fermentation residue; wherein, the first compound fermentation liquid includes Phellinus linteus, Geotrichum candida and Lactobacillus brycetes. S3. Add the fermentation residue obtained in step S2 to 3-4 times the mass of anhydrous ethanol for ultrasonic extraction, centrifuge, and collect the supernatant. S4. Mix the fermentation broth obtained in step S2 and the supernatant collected in step S3, and freeze-dry to obtain the Schisandra chinensis extract. The preparation method of the grey soybean seed extract includes the following steps: T1. Crush the soybean seeds, add 5-6 times the weight of water, adjust the pH to 5.5-6.5, add the second complex enzyme for enzymatic hydrolysis, inactivate the enzyme, cool to room temperature, and obtain the enzymatic hydrolysate; wherein, the second complex enzyme is papain and cellulase. T2. Sterilize the enzymatic hydrolysate obtained in step T1, then inoculate the enzymatic hydrolysate with the second compound fermentation culture for fermentation, sterilize, filter, and obtain fermentation broth and fermentation residue; wherein, the second compound fermentation culture includes Aspergillus cristatus and Candida lipolyticis. T3. Add the fermentation residue obtained in step T2 to 3-4 times its mass of anhydrous ethanol for ultrasonic extraction, centrifuge, and collect the supernatant. T4. Mix the fermentation broth obtained in step T2 and the supernatant collected in step T3, and freeze-dry to obtain the gray soybean seed extract.

[0007] The composition of this invention contains only three components: 4-tert-butylcyclohexanol, Schisandra chinensis extract, and Edamame seed extract. Both the Schisandra chinensis extract and the Edamame seed extract are obtained using an enzymatic hydrolysis-fermentation-ethanol extraction-drying method, resulting in good compatibility between the Schisandra chinensis extract and the Edamame seed extract and 4-tert-butylcyclohexanol. No additional emulsifiers or solubilizers are required in the composition. The preparation principle of the Schisandra chinensis extract and the Edamame seed extract is as follows: In step S1 of the preparation of Schisandra chinensis extract, a first complex enzyme including cellulase, pectinase, and papain is used to enzymatically hydrolyze Schisandra chinensis. Through the synergistic effect of cellulase degrading cell wall cellulose, pectinase degrading pectin, and papain degrading structural proteins, not only are the cell walls and protein networks of Schisandra chinensis broken down, releasing the encapsulated active ingredients, but hydrophilic impurities in the extract are also efficiently removed. That is, cellulose and pectin (strongly hydrophilic polysaccharides) are degraded into small molecule monosaccharides such as glucose and galacturonic acid, and structural proteins are hydrolyzed into small molecule short peptides and amino acids, avoiding oil-water separation of large molecule proteins and 4-tert-butylcyclohexanol. In step S2 of the preparation of Schisandra chinensis extract, the enzymatic hydrolysate is fermented using the first composite fermentation broth. The Phellinus linteus in this broth metabolizes and produces polysaccharides and flavonoids, which, combined with the active ingredients of Schisandra chinensis, enhance its antioxidant capacity. Geotrichum can convert low-activity lignan precursors in Schisandra chinensis into highly active lignan derivatives (such as schisandrin A / B), improving the anti-inflammatory effect of the composition. Lactobacillus buchneri fermentation produces lactic acid and short-chain fatty acids. These adjust the pH of the extraction system, promoting stable dissolution of active ingredients; lactic acid also assists in the metabolism of the stratum corneum, improving the efficiency of ingredient penetration and absorption. Short-chain fatty acids possess both hydrophilic groups (such as hydroxyl and carboxyl groups) and lipophilic groups (such as alkyl groups), acting as amphiphilic surfactants. They can be adsorbed and arranged at the oil-water interface, allowing lipid-soluble components to be stably dispersed in the water-soluble system, thus improving the compatibility of the composition.

[0008] In step S3 of the preparation of Schisandra chinensis extract, the fat-soluble active ingredients remaining in the fermentation residue are enriched a second time by ultrasonic extraction with anhydrous ethanol, further increasing the proportion of fat-soluble components in the extract and making it more compatible with the composition of 4-tert-butylcyclohexanol. In step S4, freeze drying avoids component agglomeration caused by high-temperature drying, and the final extract is a loose and porous powder, rather than the dense block solid of the ethanol extract. This form is easy to disperse in the formulation and can quickly form a uniform suspension system when mixed with 4-tert-butylcyclohexanol, and can exist stably without a large amount of solubilizer.

[0009] In step T1 of the preparation of edamame seed extract, because the cell wall of edamame seeds is more tightly bound to the protein network, a second complex enzyme of papain and cellulase is selected. Papain preferentially degrades the seed protein, converting it into small molecule peptides, releasing the isoflavones encapsulated by the protein, and avoiding the polar conflict between the large molecule protein and 4-tert-butylcyclohexanol. Cellulase degrades cell wall cellulose, assisting in the release of encapsulated fat-soluble components, while reducing the residue of hydrophilic polysaccharides.

[0010] In preparation step T2, *Aspergillus cristatus* (a fungus) and *Candida lipolyticis* (a yeast) are used for co-fermentation of the enzymatic hydrolysate. The β-glucosidase produced by *Aspergillus cristatus* hydrolyzes isoflavone glycosides into aglycones. The lipophilicity of the aglycones is highly compatible with 4-tert-butylcyclohexanol, solving the problem of excessive hydrophilicity of the glycosides. *Candida lipolyticis* metabolizes to produce fatty acids (such as oleic acid and linoleic acid) and lipase. The fatty acids can form a mixed lipid phase with 4-tert-butylcyclohexanol, and the lipase further promotes the dispersion of lipophilic components. Furthermore, the polysaccharide derivatives secreted by *Aspergillus cristatus* and the fatty acids produced by *Candida lipolyticis* have natural emulsifying effects, further improving the compatibility of the composition. In preparation step T3, the residual isoflavone aglycones (lipophilic) in the fermentation residue are fully extracted by ultrasonic extraction with anhydrous ethanol, increasing the proportion of isoflavone aglycones in the *Echinopsis pilosula* seed extract and further strengthening the lipophilic compatibility with 4-tert-butylcyclohexanol.

[0011] Preferably, the mass ratio of 4-tert-butylcyclohexanol, Schisandra chinensis extract and Edamame seed extract is 1:(0.02-0.04):(0.06-0.08).

[0012] Preferably, in step S1 of preparing the Schisandra chinensis extract, the amount of cellulase added is 0.3-0.6% of the mass of Schisandra chinensis, and the enzyme activity of the cellulase is 50,000-100,000 U / g; the amount of pectinase added is 0.2-0.5% of the mass of Schisandra chinensis, and the enzyme activity of the pectinase is 50,000-100,000 U / g; the amount of papain added is 0.1-0.5% of the mass of Schisandra chinensis, and the enzyme activity of the papain is 100,000-200,000 U / g; the enzymatic hydrolysis temperature is 40-45℃ and the time is 1-2 h; the enzyme inactivation temperature is 95-105℃ and the time is 15-25 min.

[0013] Preferably, in step S2 of preparing the Schisandra chinensis extract, the inoculum amount of the first compound fermentation broth is 2-7% v / v, and the total viable count of the first compound fermentation broth is (1-3) × 10⁻⁶. 9 The CFU / mL concentration of the *Phellinus linteus*, *Geotrichum candida* and *Lactobacillus brunelli* was (0.5-1):1:(1.5-2.5), and the fermentation temperature was 30-35℃ for 24-72 h.

[0014] Preferably, in step S3 of preparing the Schisandra chinensis extract, the ultrasonic extraction temperature is 35-45℃, the power is 100-300W, and the time is 20-40min.

[0015] Preferably, in step T1 of preparing the grey soybean seed extract, the amount of cellulase added is 0.2-0.5% of the mass of the grey soybean seeds, and the enzyme activity of the cellulase is 50,000-100,000 U / g; the amount of papain added is 0.2-0.5% of the mass of the grey soybean seeds, and the enzyme activity of the papain is 100,000-200,000 U / g; the enzymatic hydrolysis temperature is 50-60℃ and the time is 1-2 h.

[0016] Preferably, in step T2 of preparing the soybean seed extract, the inoculation amount of the second compound fermentation broth is 3-5% v / v, and the total viable count of the second compound fermentation broth is (1-3) × 10⁻⁶. 9 The viable cell count ratio of Aspergillus cristatus and Candida lipolyticis was (0.5-1.5):1, and the fermentation temperature was 30-35℃ for 24-72h.

[0017] Preferably, in step T3 of the preparation of the grey soybean seed extract, the ultrasonic extraction temperature is 35-45℃, the power is 100-300W, and the time is 20-40min.

[0018] Secondly, the present invention provides the application of the sensitive skin skincare composition with soothing and repairing effects described in the first aspect in the preparation of skin products.

[0019] Thirdly, a skin care product comprising the sensitive skin care composition with soothing and repairing effects described in the first aspect.

[0020] Preferably, the skin care product comprises the following components in weight percentage: 0.1-5% of the sensitive skin care composition with soothing and repairing effects in the first aspect, 2-40% of the cosmetic matrix, and the balance being water.

[0021] Preferably, the skin care products include any one of toner, cream, mask, serum, essential oil, and spray.

[0022] Compared with the prior art, the beneficial effects of the present invention are as follows: The composition of this invention contains 4-tert-butylcyclohexanol and extracts of Schisandra chinensis and Edamame seed obtained by a specific preparation method. Both the Schisandra chinensis and Edamame seed extracts are obtained using an enzymatic hydrolysis-fermentation process. The enzymatic hydrolysis process degrades strongly hydrophilic impurities, reduces the polarity of the extracts, and minimizes the polarity difference with 4-tert-butylcyclohexanol. Fermentation converts the active ingredients into lipid-soluble small molecules, matching the solubility of 4-tert-butylcyclohexanol. Furthermore, the organic acids and amino acids in the fermentation products form a self-emulsifying system, fundamentally solving the compatibility problem between 4-tert-butylcyclohexanol and the hydrophilic extract. Secondary extraction enriches the lipid-soluble components, further improving the compatibility of the Schisandra chinensis and Edamame seed extracts with 4-tert-butylcyclohexanol. Freeze-drying yields a loose, porous powder that is easily dispersed into a uniform suspension system. The composition of the present invention has good soothing and repairing effects, and can also solve the compatibility problem at the root, without the need to add additional solubilizers and emulsifiers, making it suitable for more gentle formulations and better meeting the needs of moderate to severe sensitive skin. Attached Figure Description

[0023] Figure 1 Fluorescent images of the zebrafish tail region of the compositions of Example 1 and Comparative Example 1; Figure 2 Images of human body patch application in Examples 1-7 and Comparative Examples 1-10; Figure 3 The images show actual photos of the serums used in Application Example 1, Comparative Application Example 1, and Comparative Application Example 10. Detailed Implementation

[0024] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0025] The raw materials used in the following examples and comparative examples are as follows: Schisandra chinensis: The manufacturer is Hunan Songlingtang Chinese Herbal Medicine Pieces Co., Ltd., and the product name is Schisandra chinensis. Sanghuang porus: Sanghuangporus vaninii, purchased from China General Microbiological Culture Collection Center, accession number CGMCC 5.891; Geotrichum candida: purchased from China General Microbiological Culture Collection Center, accession number CGMCC 2.1135; Lactobacillus bruneri: purchased from China General Microbiological Culture Collection Center, accession number CGMCC 1.13; Grey soybean seeds: collected in Honghe Hani and Yi Autonomous Prefecture, Yunnan Province; Aspergillus cristatus: Manufacturer: Beijing Bio-104127; Candida lipolyticis: purchased from China Industrial Microbial Culture Collection Center, accession number CICC 32696; 4-tert-Butylcyclohexanol: Manufacturer: SymSitive (Shanghai) Co., Ltd. ® 1609; The commercially available control samples used in the comparative examples are as follows: Schisandra sphennthera extract: Manufacturer: Shanghai Boshuo Industrial Co., Ltd.; Product name: Schisandra sphennthera extract. Edamame (Tephrosia purpurea) seed extract: manufactured by Givaudan, product name: NEUROPHROLINE; Unless otherwise specified, all other materials, reagents, etc. used in the examples and comparative examples are commercially available.

[0026] The preparation methods of Schisandra chinensis extract and Edamame seed extract in the soothing and repairing skin care compositions for sensitive skin in Examples 1-5 are as follows: The preparation method of the Schisandra chinensis extract includes the following steps: S1. Grind the Schisandra chinensis fruit into powder, add 5 times its weight of water, adjust the pH to 6, add the first compound enzyme for enzymatic hydrolysis, inactivate the enzyme, and cool to room temperature to obtain the enzymatic hydrolysate; wherein, the first compound enzyme includes cellulase, pectinase and papain, the amount of cellulase added is 0.5% of the weight of Schisandra chinensis fruit, and the enzyme activity of cellulase is 80000 U / g; the amount of pectinase added is 0.4% of the weight of Schisandra chinensis fruit, and the enzyme activity of pectinase is 90000 U / g; the amount of papain added is 0.2% of the weight of Schisandra chinensis fruit, and the enzyme activity of papain is 150000 U / g; the enzymatic hydrolysis temperature is 43℃ ​​and the time is 1.5h, and the enzyme inactivation temperature is 100℃ and the time is 20min; S2. Sterilize the enzymatic hydrolysate obtained in step S1, then inoculate the enzymatic hydrolysate with the first compound fermentation broth for fermentation, sterilize, and filter to obtain fermentation broth and fermentation residue; wherein, the sterilization temperature is 121℃, the sterilization time is 20min, the inoculation amount of the first compound fermentation broth is 5%v / v, and the total viable count of the first compound fermentation broth is 2×10⁻⁶. 9 CFU / mL, the first compound fermentation liquid includes Phellinus linteus, Geotrichum candida and Lactobacillus brunelli, the viable cell ratio of Phellinus linteus, Geotrichum candida and Lactobacillus brunelli is 0.7:1:2, the fermentation temperature is 32℃ and the time is 36h; S3. Add the fermentation residue obtained in step S2 to 3 times its mass of anhydrous ethanol for ultrasonic extraction, centrifuge, and collect the supernatant; wherein, the ultrasonic extraction temperature is 40℃, the power is 200W, and the time is 30min. S4. Mix the fermentation broth obtained in step S2 and the supernatant collected in step S3, and freeze-dry to obtain the Schisandra chinensis extract. The preparation method of the grey soybean seed extract includes the following steps: T1. Crush the soybean seeds, add 6 times their weight of water, adjust the pH to 5.5, add a second compound enzyme for enzymatic hydrolysis, inactivate the enzyme, and cool to room temperature to obtain the enzymatic hydrolysate; wherein, the compound enzyme is papain and cellulase, the amount of cellulase added is 0.3% of the weight of the soybean seeds, and the enzyme activity of cellulase is 80000 U / g; the amount of papain added is 0.3% of the weight of the soybean seeds, and the enzyme activity of papain is 150000 U / g; the enzymatic hydrolysis temperature is 55℃ and the time is 1.5h, and the enzyme inactivation temperature is 100℃ and the time is 20min; T2. Sterilize the enzymatic hydrolysate obtained in step T1, then inoculate the enzymatic hydrolysate with the second compound fermentation broth for fermentation, sterilize, and filter to obtain fermentation broth and fermentation residue; wherein, the sterilization temperature is 121℃, the sterilization time is 20min, the inoculation amount of the second compound fermentation broth is 4%v / v, and the total viable count of the second compound fermentation broth is 2×10⁻⁶. 9 CFU / mL, the second compound fermentation broth includes Aspergillus cristatus and Candida lipolyticis, the viable cell ratio of Aspergillus cristatus and Candida lipolyticis is 1:1, the fermentation temperature is 32℃ and the time is 36h; T3. Add the fermentation residue obtained in step T2 to 4 times its mass of anhydrous ethanol for ultrasonic extraction, centrifuge, and collect the supernatant; wherein, the ultrasonic extraction temperature is 40℃, the power is 200W, and the time is 30min. T4. Mix the fermentation broth obtained in step T2 and the supernatant collected in step T3, and freeze-dry to obtain the gray soybean seed extract.

[0027] The mass ratios of the ingredients in the soothing and repairing skincare compositions for sensitive skin in Examples 1-5 are shown in Table 1, and the total mass of each composition is equal.

[0028] The preparation method of the skin care compositions for sensitive skin with soothing and repairing effects in Examples 1-5 includes the following steps: The extracts of Schisandra chinensis and Edamame seed were mixed and then slowly added with 4-tert-butylcyclohexanol. The mixture was stirred at 45°C until a homogeneous mixture was formed.

[0029] Table 1. Mass ratio of each component in each group of skincare compositions for sensitive skin with soothing and repairing effects.

[0030] Example 6 The only difference between Example 6 and Example 1 is that in step S2 of the preparation of Schisandra chinensis extract in Example 6, the total number of viable bacteria in the first compound fermentation liquid remains unchanged, and the viable bacteria counts of Phellinus linteus, Geotrichum candida and Lactobacillus bryceae are 2:0.7:1.

[0031] Example 7 The only difference between Example 7 and Example 1 is that in step S2 of the preparation of Schisandra chinensis extract in Example 7, the total number of viable bacteria in the first compound fermentation liquid remains unchanged, and the viable bacteria counts of Phellinus linteus, Geotrichum candida and Lactobacillus bryceae are 0.7:2:1.

[0032] Comparative Example 1 The only difference between Comparative Example 1 and Example 1 is that Comparative Example 1 uses commercially available Schisandra chinensis extract instead of Schisandra chinensis extract.

[0033] Comparative Example 2 The only difference between Comparative Example 2 and Example 1 is that the preparation method of the Schisandra chinensis extract in Comparative Example 2 (without fermentation) includes the following steps: The preparation method of the Schisandra chinensis extract includes the following steps: S1. Grind the Schisandra chinensis fruit into powder, add 5 times its weight of water, adjust the pH to 6, add the first compound enzyme for enzymatic hydrolysis, inactivate the enzyme, and cool to room temperature to obtain the enzymatic hydrolysate; wherein, the first compound enzyme includes cellulase, pectinase and papain, the amount of cellulase added is 0.5% of the weight of Schisandra chinensis fruit, and the enzyme activity of cellulase is 80000 U / g; the amount of pectinase added is 0.4% of the weight of Schisandra chinensis fruit, and the enzyme activity of pectinase is 90000 U / g; the amount of papain added is 0.2% of the weight of Schisandra chinensis fruit, and the enzyme activity of papain is 150000 U / g; the enzymatic hydrolysis temperature is 43℃ ​​and the time is 1.5h, and the enzyme inactivation temperature is 100℃ and the time is 20min; S2. Sterilize the enzymatic hydrolysate obtained in step S1, then add it to 3 times its mass of anhydrous ethanol for ultrasonic extraction, centrifuge, and collect the supernatant; wherein, the ultrasonic extraction temperature is 40℃, the power is 200W, and the time is 30min. S3. Freeze-dry the supernatant collected in step S2 to obtain the Schisandra chinensis extract; Comparative Example 3 The only difference between Comparative Example 3 and Example 1 is that in step S2 of preparing the Schisandra chinensis extract in Comparative Example 3, the first compound fermentation liquid does not contain Phellinus linteus, and Geotrichum candida and Lactobacillus bryceae with a live bacteria ratio of 1:2 are used to make up the total live bacteria count.

[0034] Comparative Example 4 The only difference between Comparative Example 4 and Example 1 is that in step S2 of preparing the Schisandra chinensis extract in Comparative Example 4, the first compound fermentation liquid does not contain Geotrichum candida, and Phellinus linteus and Lactobacillus brunelli with a live bacteria ratio of 0.7:2 are used to make up the total live bacteria count.

[0035] Comparative Example 5 The only difference between Comparative Example 5 and Example 1 is that in step S2 of preparing the Schisandra chinensis extract in Comparative Example 5, Lactobacillus buchneri is not added to the first compound fermentation liquid, and Phellinus linteus and Geotrichum candida albicans with a live bacteria ratio of 0.7:1 are used to make up the total live bacteria count.

[0036] Comparative Example 6 The only difference between Comparative Example 6 and Example 1 is that Comparative Example 6 uses commercially available edamame seed extract instead of edamame seed extract.

[0037] Comparative Example 7 The only difference between Comparative Example 7 and Example 1 is that the preparation method of the grey soybean seed extract in Comparative Example 7 includes the following steps: T1. Crush the soybean seeds, add 6 times their weight of water, adjust the pH to 5.5, add a second compound enzyme for enzymatic hydrolysis, inactivate the enzyme, and cool to room temperature to obtain the enzymatic hydrolysate; wherein, the compound enzyme is papain and cellulase, the amount of cellulase added is 0.3% of the weight of the soybean seeds, and the enzyme activity of cellulase is 80000 U / g; the amount of papain added is 0.3% of the weight of the soybean seeds, and the enzyme activity of papain is 150000 U / g; the enzymatic hydrolysis temperature is 55℃ and the time is 1.5h, and the enzyme inactivation temperature is 100℃ and the time is 20min; T2. Sterilize the enzymatic hydrolysate obtained in step T1, add 4 times the mass of anhydrous ethanol for ultrasonic extraction, centrifuge, and collect the supernatant; wherein, the ultrasonic extraction temperature is 40℃, the power is 200W, and the time is 30min. T3. Freeze-dry the supernatant collected in step T2 to obtain the edamame seed extract.

[0038] Comparative Example 8 The only difference between Comparative Example 8 and Example 1 is that in step T2 of the preparation of the soybean seed extract in Comparative Example 8, Aspergillus cristatus is not added to the second compound fermentation liquid, and Candida lipolyticis is used to supplement the total number of viable bacteria.

[0039] Comparative Example 9 The only difference between Comparative Example 9 and Example 1 is that in step T2 of the preparation of the soybean seed extract in Comparative Example 9, the second compound fermentation liquid does not contain Candida lipolyticis, and Aspergillus cristatus is used to supplement the total number of viable bacteria.

[0040] Comparative Example 10 The only difference between Comparative Example 10 and Example 1 is that: Comparative Example 10 uses commercially available Schisandra sphenanthera extract instead of Schisandra sphenanthera extract, and commercially available Tephrosia apurpura seed extract instead of Tephrosia apurpura seed extract.

[0041] Test Example 1: Inhibition Test of Composition on Matrix Metalloproteinase-1 Expression Studies have shown that ultraviolet radiation causes epidermal keratinocytes to release cytokines, which can indirectly promote the expression of matrix metalloproteinase-1 (MMP-1) in dermal fibroblasts. MMP-1 is one of the key enzymes leading to the degradation of skin barrier structures (especially collagen). Its overactivity can directly weaken the supporting structure of the skin barrier and trigger a chain reaction, indirectly impairing barrier function. Therefore, the skin barrier repair effect of the aforementioned samples was evaluated by testing their inhibition rate against MMP-1.

[0042] The specific testing method is as follows: The compositions of Examples 1-7 and Comparative Examples 1-10 were added to DMEM culture medium for cells and mixed well to prepare a test sample solution with a mass concentration of 0.05%. Cells (human keratinocytes, HaCaT, purchased from the Kunming Cell Bank, Chinese Academy of Sciences) were seeded into 12-well cell culture plates, with each well containing 0.75 × 10⁶ cells. 5 Cells were collected and starved in serum-free medium for 24 hours. The starved cells were washed with PBS and then exposed to a UVB lamp for 15 minutes (irradiation intensity: 85 μW / cm²). 2 Irradiation dose: 40 mJ / cm 2 ).

[0043] Grouping: The cell-containing culture medium was divided into a sample group and a control group. The sample group was given 2 mL of DMEM high-glucose culture medium containing 0.05% of the test sample solution prepared in the example or comparative example. The control group was given only 2 mL of DMEM high-glucose culture medium and cultured for 48 h.

[0044] MMP-1 content determination: Cells were lysed according to the kit instructions (Shanghai Enzyme-Linked Biotechnology, catalog number: ml038199), and the supernatant was collected. The absorbance at 450 nm was measured by ELISA, and the MMP-1 concentration (pg / mL) was calculated.

[0045] The efficacy of skin barrier repair was assessed by calculating the MMP-1 expression inhibition rate; a higher inhibition rate indicated a better barrier repair effect. The results are shown in Table 2; the calculation formula is as follows: The inhibition rate of MMP-1 expression = (AB) / A × 100%; where A is the MMP-1 expression level in the control group and B is the MMP-1 expression level in the experimental group; specific data are shown in Table 2.

[0046] Test Example 2: Inhibition Test of Composition on Sodium Lauryl Sulfate-Induced Inflammation on the Body Surface of Zebrafish The compositions of Examples 1-7 and Comparative Examples 1-10 were added to zebrafish culture medium and mixed well to prepare a test sample solution with a mass concentration of 2%. Group setup: Blank control group (zebrafish embryo culture medium); Model control group (zebrafish embryo culture medium + 60 μg / mL sodium dodecyl sulfate); Experimental group (zebrafish embryo culture medium + 60 μg / mL sodium dodecyl sulfate + test sample solution).

[0047] Zebrafish culture medium: 2940 mg anhydrous calcium chloride, 1233 mg magnesium sulfate heptahydrate, 630 mg sodium bicarbonate, 55 mg potassium chloride, and 1 g methylene blue were diluted to 10 L with deionized water. All chemicals were of analytical grade.

[0048] Test method: Healthy zebrafish that have developed for 3 days after fertilization (selection criteria: normal morphology and developed to the blastocyst stage). At least 10 zebrafish were transferred to a group of 6-well plates for rearing, and exposed to the environment for 24 hours. Fluorescence imaging: Fluorescence images of the tail region of each group of 10 zebrafish were captured under a fluorescence microscope.

[0049] Neutrophil quantification: Data was analyzed and collected using ImageJ image processing software. The target area was precisely delineated using the rectangle tool, and the average fluorescence intensity of the selected area was measured using the grayscale intensity method, which is the number of neutrophils in zebrafish.

[0050] Table 2. Data on MMP-1 expression inhibition rate and neutrophil migration count on zebrafish body surface for each composition. Group / Performance MMP-1 expression inhibition rate / % neutrophil migration count on zebrafish body surface Example 1 54.1 13.5 Example 2 53.6 14.0 Example 3 54.8 13.0 Example 4 50.6 17.0 Example 5 52.4 15.5 Example 6 48.7 18.0 Example 7 49.1 18.5 Comparative Example 1 30.3 28.0 Comparative Example 2 33.6 25.5 Comparative Example 3 38.3 22.0 Comparative Example 4 39.7 21.5 Comparative Example 5 40.8 20.5 Comparative Example 6 32.2 27.0 Comparative Example 7 35.6 24.0 Comparative Example 8 42.2 19.5 Comparative Example 9 41.6 20.0 Comparative Example 10 26.8 33.0 Blank control group / 8.0 Model control group / 39.5 As shown in Table 2, and in conjunction with the data from Examples 1 and 2-5, when the mass ratio of 4-tert-butylcyclohexanol, Schisandra chinensis extract, and Edamame seed extract is 1:(0.02-0.04):(0.06-0.08), the soothing and anti-inflammatory effects of the composition are at a relatively good level.

[0051] Based on the data from Examples 1 and 6-7 in Table 2, the soothing and anti-inflammatory effects of the compositions in Examples 6-7 are lower than those in Example 1. This may be because the proportion of Geotrichum candida in the fermentation process of the Schisandra chinensis extract in Example 6 was too low, resulting in insufficient production of lignan derivatives (such as schisandrin A / B), which affected the soothing and anti-inflammatory effects of the composition. In Example 7, the proportion of Geotrichum candida in the fermentation process of the Schisandra chinensis extract was too high, leading to excessive degradation of lignans and a reduction in barrier repair and anti-inflammatory raw materials. This indicates that if the ratio of viable bacteria of Phellinus linteus, Geotrichum candida, and Lactobacillus brunelli is within the range of (0.5-1):1:(1.5-2.5) during the fermentation of the Schisandra chinensis extract, the synergistic effect of microorganisms during fermentation can be guaranteed, promoting the conversion and release of effective components in Schisandra chinensis.

[0052] Combination Figure 1 It can be seen that the number of neutrophils (black spots) in the tail area of ​​zebrafish treated with the composition of Example 1 was less than that in Comparative Example 1, indicating that the anti-inflammatory effect of the composition of Example 1 was better than that of Comparative Example 1, that is, the composition containing Schisandra chinensis extract after enzymatic hydrolysis-fermentation treatment has better anti-inflammatory efficacy.

[0053] Based on the data from Example 1 and Comparative Examples 1, 6, and 10 in Table 2, it can be seen that the composition of Comparative Example 10 exhibited the worst anti-inflammatory and soothing effects. This may be because the commercially available Schisandra chinensis extract and Edamame seed extract did not undergo enzymatic hydrolysis and fermentation, resulting in high levels of residual hydrophilic impurities (polysaccharides, proteins), which encapsulate the active ingredients, leading to low dissolution rates of lignans and isoflavones. The components exist in large molecular form, unable to penetrate the stratum corneum to reach the target site. Furthermore, the lack of repair-enhancing components such as fatty acids produced through enzymatic hydrolysis and fermentation affects the performance of the composition. This indicates that both Schisandra chinensis extract and Edamame seed extract require enzymatic hydrolysis and fermentation to achieve better anti-inflammatory and soothing effects in the composition.

[0054] Based on the data from Example 1 and Comparative Examples 2-5 in Table 2, it can be seen that the Schisandra chinensis extract in Comparative Example 2 only underwent enzymatic hydrolysis without fermentation, while the Schisandra chinensis extracts in Comparative Examples 3-5 lacked one type of bacteria during fermentation. The anti-inflammatory and soothing effects of their compositions were lower than those of Example 1. This may be because the Schisandra chinensis extract in Comparative Example 2 did not undergo fermentation, lacking highly active lignan derivatives and short peptides, relying solely on the original lignans to exert its basic function, resulting in a significant decrease in the anti-inflammatory and soothing properties of the composition. Furthermore, the lack of *Sanghuang* in Comparative Examples 3-5 resulted in insufficient polysaccharide-assisted lipid synthesis, leading to inadequate repair raw materials. The absence of *Geotrichum candida* prevented the conversion of lignan precursors into highly active derivatives, affecting anti-inflammatory and repair effects. The lack of *Lactobacillus brunellii* prevented the provision of metabolites such as lactic acid and short-chain fatty acids, affecting pH regulation, skin metabolism promotion, and self-emulsification, thereby reducing the repair effect. This indicates that fermentation of the enzymatic hydrolysate of Schisandra chinensis using *Sanghuang*, *Geotrichum candida*, and *Lactobacillus brunellii* can synergistically improve the anti-inflammatory and soothing properties of the composition.

[0055] Based on the data from Example 1 and Comparative Examples 7-9 in Table 2, it can be seen that the anti-inflammatory and soothing repair effects of the compositions in Comparative Examples 7-9 are lower than those in Example 1. This may be because the gray soybean seed extract in Comparative Example 7 lacks a fermentation process, resulting in the absence of highly active anti-inflammatory derivatives (such as isoflavone aglycones) produced by fermentation. It relies solely on the inherent repair and anti-inflammatory components in the raw materials, and the large molecular components have difficulty penetrating the zebrafish skin, leading to a decrease in the anti-inflammatory and soothing properties of the composition. In contrast, Comparative Examples 8-9 lack one fermentation bacteria in the fermentation process of the gray soybean seed extract. The absence of Aspergillus cristatus prevents the hydrolysis of isoflavone glycosides into lipid-soluble aglycones, making it difficult for hydrophilic glycosides to penetrate the skin and limiting their repair effect. The absence of Candida lipolytica results in the lack of fatty acid supplementation of lipids. This indicates that Aspergillus cristatus hydrolyzes isoflavone glycosides into aglycones during the fermentation process of the gray soybean seed extract, while Candida lipolytica generates fatty acids. These fatty acids play a natural emulsifying role, promoting the dispersion of active ingredients and synergistically strengthening barrier repair and anti-inflammation.

[0056] Application Example 1-7 and Comparative Application Example 1-10 The compositions of Examples 1-7 and Comparative Examples 1-10 were added to the serum at a concentration of 2 wt% to obtain the serums of Application Examples 1-7 and Comparative Application Examples 1-10. The formulations are shown in Table 3.

[0057] The preparation methods of the serums used in Application Examples 1-7 and Comparative Application Examples 1-10 include the following steps: S1. Mix the humectant, thickener and 1 / 2 volume of deionized water, and homogenize at 80°C to obtain a mixture; S2. When the temperature of the mixture in S1 drops to 60°C, add the preservative. When the temperature of the system drops to 40°C, add the composition and the remaining deionized water, stir evenly, and finally add the pH adjuster to adjust the pH to 6 to obtain the essence.

[0058] Table 3. Serum formulations for Application Examples 1-7 and Comparative Application Examples 1-10

[0059] Test Example 3: Human Patch Test of Each Group of Essences Twenty volunteers were recruited, ten men and ten women, aged 20-50 years. A closed patch test method was used. To eliminate the influence of arm position, equal volumes (1-1.1 mL) of test samples (the serums of Application Examples 1-7 and Control Application Examples 1-10) were randomly placed in a specific patch applicator. The applicator was then applied to the volunteers' arms with hypoallergenic adhesive tape. Ten sets of test samples were applied to one arm, and seven sets to the other arm. The samples were gently pressed to ensure even application to the skin, and left on for 24 hours. After 24 hours, the patch applicator was removed, and skin reactions were observed and recorded at 0.5 hours, 24 hours, and 48 hours. The severity of adverse skin reactions is shown in Table 4 below.

[0060] Table 4. Adverse skin reaction grades Rating levels Skin reaction 0 negative reaction 1 Suspicious reaction, only slight erythema 2 Weak positive reaction (erythema reaction): erythema, infiltration, edema, and papules may be present. 3 Strong positive reaction (herpes reaction): erythema, infiltration, edema, papules; the reaction may extend beyond the test area. 4 Extremely strong positive reaction (confluent herpes simplex reaction): obvious erythema, severe infiltration, edema, confluent herpes simplex; reaction extends beyond the test area. After testing, such as Figure 2 As shown, after the serums of Application Examples 1-7 and Comparative Application Examples 1-10 were tested on human skin patches, two weak positive reactions were observed in Comparative Application Examples 1, 6 and 10, one suspicious reaction was observed in Comparative Application Examples 2 and 7, and the remaining application examples were all negative. The serums are safe and non-irritating to human skin.

[0061] Test Example 4: Stability Test of Each Group of Serums Test procedure: Take 20 mL of the essence from Application Examples 1-7 and Comparative Application Examples 1-10 and place them in colorless and transparent glass bottles, then seal them. Place the sealed test samples in a standard light source D65 (6500K) at 55℃ for 4 weeks. Observe the layering of the test samples in the 4th week. The test results are shown in Table 5.

[0062] Table 5. Stability test results of each group of serums Group Layering Application Example 1 No layering Application Example 2 No layering Application Example 3 No layering Application Example 4 No layering Application Example 5 No layering Application Example 6 Slight stratification, with trace amounts of precipitation at the top. Application Example 7 Slight stratification, with trace amounts of precipitation at the top. Comparative Application Example 1 There is obvious stratification, with floating oil and top separation. Comparative Application Example 2 Clearly layered, with distinct separation between upper and lower layers. Comparative Application Example 3 Slight stratification, with a small amount of floating oil on the top layer. Comparative Application Example 4 Slight stratification, with a small amount of floating oil on the top layer. Comparative Application Example 5 Slight stratification, with trace amounts of precipitation at the top. Comparative Application Example 6 There is obvious stratification, with floating oil and top separation. Comparative Application Example 7 Clearly layered, with distinct separation between upper and lower layers. Comparative Application Example 8 Slight stratification, with a small amount of floating oil on the top layer. Comparative Application Example 9 Slight stratification, with a small amount of floating oil on the top layer. Comparative Application Example 10 Severe stratification, complete oil-water separation As shown in Table 5, combining the serum stratification results of Application Example 1 and Application Examples 6-7, the storage stability of the serum in Application Examples 6-7 is lower than that in Application Example 1. This may be because the proportion of *Lactobacillus bruneri* during the fermentation process of the *Schisandra chinensis* extract in Application Example 6 is insufficient, leading to a reduction in the production of amphiphilic components such as lactic acid and short-chain fatty acids. This results in insufficient coating of the 4-tert-butylcyclohexanol surface to form a hydrophilic protective film, causing some 4-tert-butylcyclohexanol oil droplets to aggregate and float to the surface. Conversely, the proportion of *Geotrichum candida* during the fermentation process of the *Schisandra chinensis* extract in Application Example 7 is too high, leading to excessive degradation of lignans and a slight decrease in the proportion of fat-soluble components, thus weakening the component matching with 4-tert-butylcyclohexanol. This indicates that if the ratio of viable bacteria of *Schisandra chinensis*, *Geotrichum candida*, and *Lactobacillus bruneri* during the fermentation process of the *Schisandra chinensis* extract is within the range of (0.5-1):1:(1.5-2.5), the stability of the serum is at a relatively optimal level.

[0063] Combining the serum layering results of Application Example 1 and Comparative Application Examples 1, 6, and 10, it can be seen that the storage stability of the serums in Comparative Application Examples 1, 6, and 10 is lower than that in Application Example 1. This may be because Comparative Application Example 1 uses commercially available Schisandra chinensis extract, while Comparative Application Example 6 uses commercially available Edamame seed extract. Commercially available extracts are mostly simple alcohol extracts, with a high amount of strongly hydrophilic cellulose, pectin, and large molecular protein residues. The strongly hydrophilic components create a polar conflict with the lipid solubility of 4-tert-butylcyclohexanol. Moreover, the active ingredients of Schisandra chinensis and Edamame seed are large molecules (lignan precursors, isoflavone glycosides) without small molecule modification, resulting in a low proportion of lipid-soluble components, which does not match the composition of 4-tert-butylcyclohexanol. Furthermore, they lack natural stabilizing substances such as lactic acid and fatty acids produced by enzymatic hydrolysis and fermentation. Due to the lack of emulsifying and solubilizing components in the serum system, 4-tert-butylcyclohexanol and the two extracts will separate into oil and water. This indicates that the combination of Schisandra chinensis extract and Edamame seed extract, after enzymatic hydrolysis and fermentation respectively, with 4-tert-butylcyclohexanol is the key factor in improving the stability of the essence.

[0064] Combining the serum stratification results of Application Example 1 and Comparative Application Examples 2-5, it can be seen that the storage stability of the serum in Comparative Application Examples 2-5 is lower than that in Application Example 1. This may be because the Schisandra chinensis extract in Comparative Application Example 2 did not undergo fermentation. Although enzymatic hydrolysis degraded some large molecular impurities, it did not convert the active ingredients into fat-soluble small molecules (such as lignan derivatives) through fermentation. The extract is still hydrophilic, and the polar conflict with 4-tert-butylcyclohexanol has not been fundamentally resolved. In contrast, the Schisandra chinensis extract in Comparative Application Examples 3-5 lacked certain fermentation bacteria during the fermentation process. This may result in: the lack of Phellinus linteus leading to the absence of additional polysaccharides to stabilize the system, resulting in weakened dispersibility; the lack of Geotrichum candida preventing the conversion of lignan precursors into highly active fat-soluble derivatives, resulting in insufficient fat-soluble components and decreased compatibility with 4-tert-butylcyclohexanol; and the lack of Lactobacillus brucellosis preventing the formation of amphiphilic components such as lactic acid and short-chain fatty acids, making it easy for 4-tert-butylcyclohexanol oil droplets to aggregate. This indicates that the Schisandra chinensis extract, through synergistic fermentation with Geotrichum can improve the compatibility of the composition with 4-tert-butylcyclohexanol.

[0065] Combining the serum stratification results of Application Example 1 and Comparative Application Examples 7-9, it can be seen that the storage stability of the serum in Comparative Application Examples 7-9 is lower than that in Application Example 1. This may be because the soybean seed extract in Comparative Application Example 7 did not undergo fermentation treatment, resulting in higher impurity residues and incomplete resolution of the polar conflict with 4-tert-butylcyclohexanol. In contrast, the soybean seed extract in Comparative Application Examples 8-9 lacked certain fermentation bacteria during fermentation. The absence of *Aspergillus cristatus* prevented the production of β-glucosidase, hindering the hydrolysis of isoflavone glycosides into lipophilic aglycones, increasing the residue of hydrophilic glycosides, and still maintaining the polar conflict with 4-tert-butylcyclohexanol. Furthermore, the absence of *Candida lipolytica* prevented the production of fatty acids in the system, hindering oil-water dispersion and reducing the dispersion stability of the serum. Therefore, *Aspergillus cristatus* hydrolyzes isoflavone glycosides into lipophilic aglycones, forming a mixed oil phase with 4-tert-butylcyclohexanol. The fatty acids produced by *Candida lipolytica* act as natural emulsifiers, and the synergistic fermentation of both processes further improves the stability of the soybean seed extract.

[0066] Depend on Figure 3 It can be seen that the essence of Application Example 1 did not show stratification under specific storage conditions, while Comparative Application Example 1 showed stratification with floating oil and top precipitation. The most serious case was the essence of Comparative Application Example 10, which showed severe oil-water separation. This indicates that the extracts of Schisandra chinensis and Edamame seed obtained by the specific preparation method are the key factors affecting the stability of the essence.

[0067] In summary, this invention actively modifies Schisandra chinensis extract and Edamame seed extract through enzymatic hydrolysis and fermentation. Enzymatic hydrolysis releases active ingredients and removes hydrophilic impurities, while fermentation achieves the reduction of component size and enhances lipid solubility. At the same time, it generates natural emulsifying components, achieving essential compatibility with 4-tert-butylcyclohexanol from the source. Therefore, there is no need to add emulsifiers, oils, polyols, or other components to the composition to improve the compatibility of Schisandra chinensis extract, Edamame seed extract, and 4-tert-butylcyclohexanol. It can be directly adapted to various dosage forms such as water-based, alcohol-free, and refreshing types (e.g., toners, serums), and the formula texture can be adjusted according to sensitive skin type (dry sensitive skin, oily sensitive skin).

[0068] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A skincare composition for sensitive skin with soothing and repairing effects, characterized in that, It is composed of 4-tert-butylcyclohexanol, Schisandra chinensis extract and Edamame seed extract, wherein the mass ratio of 4-tert-butylcyclohexanol, Schisandra chinensis extract and Edamame seed extract is 1:(0.01-0.1):(0.01-0.1). The preparation method of the Schisandra chinensis extract includes the following steps: S1. Crush Schisandra chinensis, add 5-6 times its weight of water, adjust the pH to 5.5-6.5, add the first complex enzyme for enzymatic hydrolysis, inactivate the enzyme, cool to room temperature, and obtain the enzymatic hydrolysate; wherein, the first complex enzyme includes cellulase, pectinase and papain. S2. Sterilize the enzymatic hydrolysate obtained in step S1, then inoculate the enzymatic hydrolysate with the first compound fermentation liquid for fermentation, sterilize, filter, and obtain fermentation liquid and fermentation residue; wherein, the first compound fermentation liquid includes Phellinus linteus, Geotrichum candida and Lactobacillus brycetes. S3. Add the fermentation residue obtained in step S2 to 3-4 times the mass of anhydrous ethanol for ultrasonic extraction, centrifuge, and collect the supernatant. S4. Mix the fermentation broth obtained in step S2 and the supernatant collected in step S3, and freeze-dry to obtain the Schisandra chinensis extract. The preparation method of the grey soybean seed extract includes the following steps: T1. Crush the soybean seeds, add 5-6 times the weight of water, adjust the pH to 5.5-6.5, add the second complex enzyme for enzymatic hydrolysis, inactivate the enzyme, cool to room temperature, and obtain the enzymatic hydrolysate; wherein, the second complex enzyme is papain and cellulase. T2. Sterilize the enzymatic hydrolysate obtained in step T1, then inoculate the enzymatic hydrolysate with the second compound fermentation culture for fermentation, sterilize, filter, and obtain fermentation broth and fermentation residue; the second compound fermentation culture includes Aspergillus cristatus and Candida lipolyticis. T3. Add the fermentation residue obtained in step T2 to 3-4 times its mass of anhydrous ethanol for ultrasonic extraction, centrifuge, and collect the supernatant. T4. Mix the fermentation broth obtained in step T2 and the supernatant collected in step T3, and freeze-dry to obtain the edamame seed extract.

2. The skincare composition for sensitive skin with soothing and repairing effects as described in claim 1, characterized in that, The mass ratio of 4-tert-butylcyclohexanol, Schisandra chinensis extract and Edamame seed extract is 1:(0.02-0.04):(0.06-0.08).

3. The skincare composition for sensitive skin with soothing and repairing effects as described in claim 1, characterized in that, In step S1 of preparing the Schisandra chinensis extract, the amount of cellulase added is 0.3-0.6% of the mass of Schisandra chinensis, and the enzyme activity of cellulase is 50,000-100,000 U / g; the amount of pectinase added is 0.2-0.5% of the mass of Schisandra chinensis, and the enzyme activity of pectinase is 50,000-100,000 U / g; the amount of papain added is 0.1-0.5% of the mass of Schisandra chinensis, and the enzyme activity of papainase is 100,000-200,000 U / g; the enzymatic hydrolysis temperature is 40-45℃ and the time is 1-2 hours.

4. The skincare composition for sensitive skin with soothing and repairing effects as described in claim 1, characterized in that, In step S2 of preparing the Schisandra chinensis extract, the inoculum size of the first compound fermentation broth is 2-7% v / v, and the total viable count of the first compound fermentation broth is (1-3) × 10⁻⁶. 9 The CFU / mL concentration of the *Phellinus linteus*, *Geotrichum candida* and *Lactobacillus brunelli* was (0.5-1):1:(1.5-2.5), and the fermentation temperature was 30-35℃ for 24-72 h.

5. The skincare composition for sensitive skin with soothing and repairing effects as described in claim 1, characterized in that, In step T1 of preparing the grey soybean seed extract, the amount of cellulase added is 0.2-0.5% of the mass of grey soybean seeds, and the enzyme activity of cellulase is 50,000-100,000 U / g; the amount of papain added is 0.2-0.5% of the mass of grey soybean seeds, and the enzyme activity of papain is 100,000-200,000 U / g; the enzymatic hydrolysis temperature is 50-60℃ and the time is 1-2 h.

6. The skincare composition for sensitive skin with soothing and repairing effects as described in claim 1, characterized in that, In step T2 of the preparation of soybean seed extract, the inoculation amount of the second compound fermentation broth is 3-5% v / v, and the total viable count of the second compound fermentation broth is (1-3) × 10⁻⁶. 9 The viable cell count ratio of Aspergillus cristatus and Candida lipolyticis was (0.5-1.5):1, and the fermentation temperature was 30-35℃ for 24-72h.

7. The use of a skin care composition for sensitive skin with soothing and repairing effects as described in any one of claims 1-6 in the preparation of skin care products.

8. A skin product, characterized in that, The skin care products include sensitive skin skin care compositions with soothing and repairing effects as described in any one of claims 1-6.

9. The skin product as described in claim 8, characterized in that, The skin care product comprises the following components in weight percentage: 0.1-5% of the sensitive skin care composition with soothing and repairing effects as described in any one of claims 1-6, 2-40% of the cosmetic base, and the balance being water.

10. The skin product as described in claim 9, characterized in that, The skin care products include any one of the following: toner, cream, face mask, serum, facial oil, and spray.

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