Method for detecting functional active components in Chinese whisky

Abstract

The invention belongs to the technical field of food detection and analysis, and particularly relates to a method for detecting functional active components in Chinese whisky. In order to rapidly and accurately detect the active components and the content of the active components in the Chinese whisky, the Chinese whisky is taken as a research object, an ultra-high performance liquid chromatography-mass spectrometry method is adopted, and chromatography and mass spectrometry detection conditions are optimized, so that the beneficial components ellagic acid and 5-hydroxymethylfurfural in the Chinese whisky are efficiently separated; and detecting the contents of the two by combining a high performance liquid chromatography external standard method. The detection method disclosed by the invention is simple in pretreatment step, high in precision and good in stability, and can be used for simultaneously and rapidly determining the components in the traditional Chinese medicinal material mixed extracting solution of the Chinese whisky and similar compound wine or formula.

Description

Technical Field

[0001] This invention belongs to the field of food testing and analysis technology, specifically relating to a method for detecting functional active ingredients in Chinese whisky. Background Technology

[0002] Whisky is a distilled spirit made from malt and grains through saccharification, fermentation, distillation, aging, and blending. Current research largely focuses on strong-aroma baijiu (Chinese white liquor) or traditional whisky made solely from malted barley. Chinese-style whisky, a fusion of strong-aroma baijiu and traditional whisky, refers to spirits produced in China that follow or borrow whisky-making techniques while incorporating uniquely Chinese ingredients, flavor elements, or aging philosophies. Due to the use of Chinese grains, fermenting agents, and localized aging processes, the final whisky exhibits significantly different types and amounts of flavor compounds compared to traditional whisky, thus creating a new product with a unique flavor. However, there are currently few reports on the component analysis of Chinese-style whisky, resulting in a lack of clear indicators for quality control in its production. Summary of the Invention

[0003] To rapidly and accurately detect the active ingredients and their content in Chinese whisky, this invention focuses on Chinese whisky and employs ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS / MS) to analyze its beneficial components, combined with external standard method in HPLC to determine the content of these beneficial components. This method provides data support for the product quality control and market promotion of Chinese whisky, and establishes a rapid and highly precise analytical method for simultaneously detecting multiple active ingredients in Chinese whisky, allowing for a perfect interpretation and expression of its nutritional value.

[0004] To achieve the above-mentioned objectives, the technical solution adopted in this application is as follows: This invention provides a method for detecting functional active ingredients in Chinese whisky, comprising the following steps: (1) Preparation of the test sample: After filtering the Chinese whisky, the test sample is obtained; (2) Preparation of single standard solutions: prepare single standard solutions of different active ingredients in a gradient; (3) Detection of the test sample: The active ingredients in the test sample were qualitatively analyzed by ultra-high performance liquid chromatography-mass spectrometry and quantitatively analyzed by high performance liquid chromatography with external standard method. The active ingredients are ellagic acid and 5-hydroxymethylfurfural; For qualitative analysis, the chromatographic conditions are as follows: The chromatographic column was a Thermo Scientific Accucore TMC18; Mobile phase A is a 0.1% formic acid acetonitrile solution, and mobile phase B is a 0.1% formic acid aqueous solution; Elution conditions: 0 to 10 min, 5 to 20% A; 10 to 15 min, 20 to 40% A; 15 to 25 min, 40 to 40% A; 25 to 50 min, 40 to 100% A; 50 to 60 min, 100 to 100% A; 60 to 60.1 min, 100 to 5% A; The mass spectrometry conditions are as follows:

[0005] For quantitative analysis, the chromatographic conditions are as follows: The chromatographic column was a ZORBAX Eclipse Plus C18; Mobile phase A is a 0.1% formic acid acetonitrile solution, and mobile phase B is a 0.1% formic acid aqueous solution; Elution conditions: 0~6 min, 5~12.5% ​​A; 6~8 min, 12.5~20% A; 8~14 min, 20~25.5% A; 14~16 min, 25.5~100% A; 16~17 min, 100~100% A; 17~18 min, 100~5% A; 18~20 min, 5~5% A.

[0006] Furthermore, the Chinese-style whiskey is filtered through a 0.22 μm filter membrane.

[0007] Furthermore, for qualitative analysis, the chromatographic conditions include: Thermo Scientific Accucore TMC18 column model is selected from at least one of the following: 2.6 μm, 3*100 mm, 2.6 μm, 2.1*100 mm, 2.6 μm, 4.6*150 mm, 2.6 μm, 4.6*50 mm. Detection wavelength: 280 nm; Column temperature 40–42 ℃; Flow rate: 0.1–0.4 mL / min; Injection volume: 1–4 μL.

[0008] Furthermore, for qualitative analysis, mass spectrometry conditions include: Electrospray ionization source; Detection in positive and negative ion modes; Spray voltage: 3.2 kV; Ion source temperature 350℃; Both the sheath gas and the auxiliary gas are nitrogen. Sheath flow velocity 35 arb; Auxiliary airflow velocity 10 arb; Ion transfer tube temperature 320℃; The scanning mode is a full scan, with data dependent on a secondary scan. Primary resolution: 70,000; Secondary resolution: 17,500. Scan range 100–1500 m / z; The collision energy gradients are 20 eV, 40 eV, and 60 eV.

[0009] Furthermore, for quantitative analysis, the chromatographic conditions include: The ZORBAX Eclipse Plus C18 chromatographic model is selected from at least one of the following: 5 μm, 4.6*250 mm, 5 μm, 4.6*150 mm, 5 μm, 4.6*100 mm, 5 μm, 4.6*50 mm, 5 μm, 2.1*100 mm, 5 μm, 2.1*50 mm. Detection wavelength: 280 nm; Column temperature 28–32℃; Flow rate: 0.8–1.2 mL / min; Injection volume: 8–12 μL.

[0010] Furthermore, the quantitative analysis involves plotting a standard curve using the peak area and concentration of standard working solutions at different concentrations as parameters. Then, by substituting the peak area corresponding to the active ingredient in the sample under the same chromatographic conditions into the standard curve, the content of each component to be tested in Chinese whisky can be obtained.

[0011] Beneficial Effects: This invention employs ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS / MS) to qualitatively analyze the active components in Chinese whisky by optimizing chromatographic and mass spectrometric parameters. Quantitative analysis is then performed using external standard method in HPLC, enabling accurate and rapid detection of ellagic acid and 5-hydroxymethylfurfural in Chinese whisky and their respective contents. The detection method of this invention features simple pretreatment steps, high precision, and good stability, and can be used for the simultaneous and rapid determination of components in mixed extracts of Chinese medicinal herbs in Chinese whisky and similar blended spirits or formulations. Attached Figure Description

[0012] Figure 1 This is the linear regression equation for ellagic acid and 5-hydroxymethylfurfural obtained by the detection method of this invention. Detailed Implementation

[0013] To make the technical problems, solutions, and beneficial effects of this application clearer, the following detailed description is provided in conjunction with the embodiments. Unless otherwise defined, all technical terms used herein have the same meaning as understood by one of ordinary skill in the art.

[0014] This invention employs ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS / MS) for qualitative analysis of active components in Chinese whisky, followed by quantitative analysis using high performance liquid chromatography with external standard method, thereby accurately and rapidly detecting ellagic acid and 5-hydroxymethylfurfural and their content in Chinese whisky.

[0015] In some specific embodiments of the present invention, for qualitative analysis, the liquid chromatography-mass spectrometry (LC-MS) instrument used is a Vanquish ultra-high performance liquid chromatography-QExactive quadrupole-electrostatic field orbital trap high-resolution mass spectrometer. The acquired raw data is imported into the Compound Discoverer 3.2 mass spectrometry data processing software, and combined with the primary quasi-molecular ion peak mass-to-charge ratio (m / z), secondary fragment ion information, and the mzCloud online database and mzVault local database, precise qualitative analysis of the components is performed. For the high performance liquid chromatography external standard method, the instrument used is an Agilent 1260 Infinity II liquid chromatograph. A standard curve is plotted using standard samples corresponding to the analyte. Under the same chromatographic conditions, each component of the analyte sample is substituted into the standard curve to achieve quantitative analysis of the analyte.

[0016] The optimized mass spectrometry conditions are as follows:

[0017] Since the elution phase and its ratio directly affect the separation degree and peak time of the active ingredients, this invention optimizes the elution conditions based on the chemical properties of the two active ingredients to be measured, selecting formic acid acetonitrile solution and formic acid aqueous solution as the mobile phase, and optimizing the elution conditions to ensure that ellagic acid and 5-hydroxymethylfurfural in Chinese whisky can be fully separated. Therefore, in some specific embodiments of this invention, the chromatographic conditions in the ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS / MS) method are as follows: The chromatographic column was a Thermo Scientific Accucore TMC18; Mobile phase A is a 0.1% formic acid acetonitrile solution, and mobile phase B is a 0.1% formic acid aqueous solution; Elution conditions: 0 to 10 min, 5 to 20% A; 10 to 15 min, 20 to 40% A; 15 to 25 min, 40 to 40% A; 25 to 50 min, 40 to 100% A; 50 to 60 min, 100 to 100% A; 60 to 60.1 min, 100 to 5% A.

[0018] Because the alcoholic beverage may contain other non-pure liquid substances or impurities, filtration is performed before injection. This avoids these substances affecting the chromatographic column and causing inaccurate peak elution, and also significantly extends the column's lifespan. Due to the extremely high system pressure and small column pore size of the ultra-high performance liquid chromatography (UHPLC) used, the sample requires high cleanliness. To better remove impurities, in some specific embodiments of this invention, the Chinese whisky is filtered through a 0.22 μm filter membrane.

[0019] The external standard method in high-performance liquid chromatography (HPLC) involves plotting a working curve using standard samples corresponding to the analytes. Specifically, a standard curve is constructed by plotting the peak areas of each standard sample at different concentrations in the extraction ion chromatograms obtained by HPLC. Then, the peak areas corresponding to each component in the extraction ion chromatograms of the analytes under the same chromatographic conditions are substituted into the standard curve to obtain the actual concentration of each analyte in Chinese whisky.

[0020] In alcoholic beverages, acids contribute acidity and a certain level of mouthfeel, while aldehydes and ketones typically contribute to aroma and mouthfeel, imparting fruity, vanilla, or nutty notes. Qualitative analysis revealed that ellagic acid and 5-hydroxymethylfurfural were present in the highest concentrations of these two compounds, respectively. Therefore, when determining the content of active ingredients using high-performance liquid chromatography (HPLC) with external standard method, standard working solutions of ellagic acid and 5-hydroxymethylfurfural were prepared. These standard working solutions were all dissolved in methanol, under which conditions the solubility of the standard samples of ellagic acid and 5-hydroxymethylfurfural in this solvent was optimal.

[0021] The following specific embodiments will be provided to explain the solution of the present invention. Those skilled in the art will understand that the following embodiments are for illustrative purposes only and should not be considered as limiting the scope of the invention. Where specific techniques or conditions are not specified in the embodiments, they are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. Reagents or instruments whose manufacturers are not specified are all conventional products that can be obtained commercially.

[0022] Example 1 Commercially available Chinese whisky was filtered through a 0.22 μm filter membrane and then analyzed using ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) to obtain the extracted ion chromatogram of the Chinese whisky.

[0023] Chromatographic conditions: The chromatographic column was a Thermo Scientific Accucore TMC18 (2.6 μm, 3 mm * 100 mm); mobile phase A was 0.1% formic acid acetonitrile solution, and mobile phase B was 0.1% formic acid aqueous solution; elution conditions: 0–10 min, 5–20% A; 10–15 min, 20–40% A; 15–25 min, 40–40% A; 25–50 min, 40–100% A; 50–60 min, 100–100% A; 60–60.1 min, 100–5% A; detection wavelength: 280 nm; column temperature: 40 ℃; flow rate: 0.3 mL / min; injection volume: 3 μL.

[0024] Mass spectrometry conditions: Electrospray ionization (ESI) is used for detection in both positive and negative ion modes.

[0025] The spray voltage was 3.2 kV, the ion source temperature was 350℃, and nitrogen was used for all gases. The sheath gas flow rate was 35 arb, the auxiliary gas flow rate was 10 arb, and the ion transmission tube temperature was 320℃. The scanning mode was full scan data-dependent two-stage scan (FullMS / dd-MS2), with a primary resolution of 70,000 and a secondary resolution of 17,500. The scan range was 100–1500 m / z, and the collision energy gradients were 20 eV, 40 eV, and 60 eV.

[0026] Ellagic acid and 5-hydroxymethylfurfural standards were transferred to 5 mL or 10 mL volumetric flasks and diluted to 100 mL with methanol to prepare a stock solution. This stock solution was then gradually diluted using a methanol gradient to prepare ellagic acid and 5-hydroxymethylfurfural standards at concentrations of 5, 10, 20, 40, and 80 μg / mL, and 5-hydroxymethylfurfural standards at concentrations of 1.25, 2.5, 5, 10, and 20 μg / mL. High-performance liquid chromatography (HPLC) with external standard method was used for detection, and chromatograms of the standard working solutions at different concentrations were obtained. Standard curves for ellagic acid and 5-hydroxymethylfurfural standards were then plotted.

[0027] Chromatographic conditions: The chromatographic column was a ZORBAX Eclipse Plus C18 (5 μm, 4.6 mm * 250 mm); mobile phase A was 0.1% formic acid acetonitrile solution, and mobile phase B was 0.1% formic acid aqueous solution; elution conditions: 0–6 min, 5–12.5% ​​A; 6–8 min, 12.5–20% A; 8–14 min, 20–25.5% A; 14–16 min, 25.5–100% A; 16–17 min, 100–100% A; 17–18 min, 100–5% A; 18–20 min, 5–5% A; detection wavelength 280 nm; column temperature 30℃; flow rate 1 mL / min; injection volume 10 μL.

[0028] Based on the standard curves of ellagic acid and 5-hydroxymethylfurfural standards, the actual content of ellagic acid and 5-hydroxymethylfurfural in Chinese whisky was calculated using the external standard method.

[0029] Meanwhile, ellagic acid and 5-hydroxymethylfurfural standards with a mass concentration of 10 μg / mL were added to the whisky samples to be tested. Six parallel samples were prepared for each level, and the spiked recovery rate was calculated.

[0030] The qualitative and quantitative parameters of ellagic acid and 5-hydroxymethylfurfural are shown in Table 1, and the chemical names and structural information of the two active ingredients are shown in Table 2.

[0031] Table 1 Qualitative parameters of the two active ingredients

[0032] Table 2 Chemical names and structural information of the two active ingredients

[0033] The two components determined by the method of this invention exhibit a good linear relationship within the linear range, with a correlation coefficient R0. 2 All values ​​were greater than 0.9986, and the spiked recoveries ranged from 85.30% to 96.96%, indicating that the detection method provided by this invention has high accuracy. The linear regression equations and spiked recoveries for ellagic acid and 5-hydroxymethylfurfural are shown below. Figure 1 As shown in Tables 3, 4 and 5.

[0034] Table 3. Linear regression equations for ellagic acid and 5-hydroxymethylfurfural

[0035] Table 4. Ellagic acid spiked recovery rate

[0036] Table 5. Spiked recoveries of 5-hydroxymethylfurfural

[0037] The detection method of the present invention was used to conduct repeated tests, and the same Chinese whisky sample was measured 6 times. The results are shown in Table 6, which shows that the method has good repeatability.

[0038] Table 6. Content of ellagic acid and 5-hydroxymethylfurfural in Chinese whisky (μg / mL)

[0039] Example 2 Commercially available Chinese whisky was filtered through a 0.22 μm filter membrane and then analyzed using ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) to obtain the extracted ion chromatogram of the Chinese whisky.

[0040] Chromatographic conditions: The chromatographic column was a Thermo Scientific Accucore TMC18 (2.6 μm, 3 mm * 100 mm); mobile phase A was 0.1% formic acid acetonitrile solution, and mobile phase B was 0.1% formic acid aqueous solution; elution conditions: 0–10 min, 5–20% A; 10–15 min, 20–40% A; 15–25 min, 40–40% A; 25–50 min, 40–100% A; 50–60 min, 100–100% A; 60–60.1 min, 100–5% A; detection wavelength: 280 nm; column temperature: 40 ℃; flow rate: 0.3 mL / min; injection volume: 3 μL.

[0041] Mass spectrometry conditions: Electrospray ionization (ESI) is used for detection in both positive and negative ion modes.

[0042] The spray voltage was 3.2 kV, the ion source temperature was 350℃, and nitrogen was used for all gases. The sheath gas flow rate was 35 arb, the auxiliary gas flow rate was 10 arb, and the ion transmission tube temperature was 320℃. The scanning mode was full scan data-dependent two-stage scan (FullMS / dd-MS2), with a primary resolution of 70,000 and a secondary resolution of 17,500. The scan range was 100–1500 m / z, and the collision energy gradients were 20 eV, 40 eV, and 60 eV.

[0043] Ellagic acid and 5-hydroxymethylfurfural standards were transferred to 5 mL or 10 mL volumetric flasks and diluted to 100 mL with methanol to prepare a stock solution. This stock solution was then gradually diluted using a methanol gradient to prepare ellagic acid and 5-hydroxymethylfurfural standards at concentrations of 5, 10, 20, 40, and 80 μg / mL, and 5-hydroxymethylfurfural standards at concentrations of 1.25, 2.5, 5, 10, and 20 μg / mL. High-performance liquid chromatography (HPLC) with external standard method was used for detection, and chromatograms of the standard working solutions at different concentrations were obtained. Standard curves for ellagic acid and 5-hydroxymethylfurfural standards were then plotted.

[0044] Chromatographic conditions: The chromatographic column was a ZORBAX Eclipse Plus C18 (5 μm, 4.6 mm * 250 mm); mobile phase A was 0.1% formic acid acetonitrile solution, and mobile phase B was 0.1% formic acid aqueous solution; elution conditions: 0–6 min, 5–12.5% ​​A; 6–8 min, 12.5–20% A; 8–14 min, 20–25.5% A; 14–16 min, 25.5–100% A; 16–17 min, 100–100% A; 17–18 min, 100–5% A; 18–20 min, 5–5% A; detection wavelength 280 nm; column temperature 28℃; flow rate 0.8 mL / min; injection volume 8 μL.

[0045] Based on the standard curves of ellagic acid and 5-hydroxymethylfurfural standards, the actual content of ellagic acid and 5-hydroxymethylfurfural in Chinese whisky was calculated using the external standard method.

[0046] Example 3 Commercially available Chinese whisky was filtered through a 0.22 μm filter membrane and then analyzed using ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) to obtain the extracted ion chromatogram of the Chinese whisky.

[0047] Chromatographic conditions: The chromatographic column was a Thermo Scientific Accucore TMC18 (2.6 μm, 3 mm * 100 mm); mobile phase A was 0.1% formic acid acetonitrile solution, and mobile phase B was 0.1% formic acid aqueous solution; elution conditions: 0–10 min, 5–20% A; 10–15 min, 20–40% A; 15–25 min, 40–40% A; 25–50 min, 40–100% A; 50–60 min, 100–100% A; 60–60.1 min, 100–5% A; detection wavelength: 280 nm; column temperature: 40 ℃; flow rate: 0.3 mL / min; injection volume: 3 μL.

[0048] Mass spectrometry conditions: Electrospray ionization (ESI) is used for detection in both positive and negative ion modes.

[0049] The spray voltage was 3.2 kV, the ion source temperature was 350℃, and nitrogen was used for all gases. The sheath gas flow rate was 35 arb, the auxiliary gas flow rate was 10 arb, and the ion transmission tube temperature was 320℃. The scanning mode was full scan data-dependent two-stage scan (FullMS / dd-MS2), with a primary resolution of 70,000 and a secondary resolution of 17,500. The scan range was 100–1500 m / z, and the collision energy gradients were 20 eV, 40 eV, and 60 eV.

[0050] Ellagic acid and 5-hydroxymethylfurfural standards were transferred to 5 mL or 10 mL volumetric flasks and diluted to 100 mL with methanol to prepare a stock solution. This stock solution was then gradually diluted using a methanol gradient to prepare ellagic acid and 5-hydroxymethylfurfural standards at concentrations of 5, 10, 20, 40, and 80 μg / mL, and 5-hydroxymethylfurfural standards at concentrations of 1.25, 2.5, 5, 10, and 20 μg / mL. High-performance liquid chromatography (HPLC) with external standard method was used for detection, and chromatograms of the standard working solutions at different concentrations were obtained. Standard curves for ellagic acid and 5-hydroxymethylfurfural standards were then plotted.

[0051] Chromatographic conditions: The column was a ZORBAX Eclipse Plus C18 (5 μm, 4.6 mm * 250 mm); mobile phase A was 0.1% formic acid acetonitrile solution, and mobile phase B was 0.1% formic acid aqueous solution; elution conditions: 0–6 min, 5–12.5% ​​A; 6–8 min, 12.5–20% A; 8–14 min, 20–25.5% A; 14–16 min, 25.5–100% A; 16–17 min, 100–100% A; 17–18 min, 100–5% A; 18–20 min, 5–5% A; detection wavelength: 280 nm; column temperature: 32℃; flow rate: 1.2 mL / min; injection volume: 12 μL.

[0052] Based on the standard curves of ellagic acid and 5-hydroxymethylfurfural standards, the actual content of ellagic acid and 5-hydroxymethylfurfural in Chinese whisky was calculated using the external standard method.

Claims

1. A method for detecting functional active ingredients in Chinese whisky, characterized in that, Includes the following steps: (1) Preparation of the test sample: After filtering the Chinese whisky, the test sample is obtained; (2) Preparation of single standard solutions: prepare single standard solutions of different active ingredients in a gradient; (3) Detection of the test sample: The active ingredients in the test sample were qualitatively analyzed by ultra-high performance liquid chromatography-mass spectrometry and quantitatively analyzed by high performance liquid chromatography with external standard method. The active ingredients are ellagic acid and 5-hydroxymethylfurfural; For qualitative analysis, the chromatographic conditions are as follows: The chromatographic column was a Thermo Scientific Accucore TMC18; Mobile phase A is a 0.1% formic acid acetonitrile solution, and mobile phase B is a 0.1% formic acid aqueous solution; Elution conditions: 0 to 10 min, 5 to 20% A; 10 to 15 min, 20 to 40% A; 15 to 25 min, 40 to 40% A; 25 to 50 min, 40 to 100% A; 50 to 60 min, 100 to 100% A; 60 to 60.1 min, 100 to 5% A; For qualitative analysis, the mass spectrometry conditions are as follows: For quantitative analysis, the chromatographic conditions are as follows: The chromatographic column was a ZORBAX Eclipse Plus C18; Mobile phase A is a 0.1% formic acid acetonitrile solution, and mobile phase B is a 0.1% formic acid aqueous solution; Elution conditions: 0~6 min, 5~12.5% ​​A; 6~8 min, 12.5~20% A; 8~14 min, 20~25.5% A; 14~16 min, 25.5~100% A; 16~17 min, 100~100% A; 17~18 min, 100~5% A; 18~20 min, 5~5% A.

2. The detection method according to claim 1, characterized in that, The Chinese-style whiskey is filtered through a 0.22 μm filter membrane.

3. The detection method according to claim 1, characterized in that, For qualitative analysis, the Thermo Scientific Accucore TMC18 column model is selected from at least one of the following: 2.6 μm, 3*100 mm, 2.6 μm, 2.1*100 mm, 2.6 μm, 4.6*150 mm, 2.6 μm, 4.6*50 mm.

4. The detection method according to claim 1, characterized in that, For qualitative analysis, chromatographic conditions include: Detection wavelength: 280 nm; Column temperature 40–42 ℃; Flow rate: 0.1–0.4 mL / min; Injection volume: 1–4 μL.

5. The detection method according to claim 1, characterized in that, For qualitative analysis, mass spectrometry conditions include at least one of the following: Electrospray ionization source; Detection in positive and negative ion modes; Spray voltage: 3.2 kV; Ion source temperature 350℃; Both the sheath gas and the auxiliary gas are nitrogen. Sheath flow velocity 35 arb; Auxiliary airflow velocity 10 arb; Ion transfer tube temperature 320℃; The scanning mode is a full scan, with data dependent on a secondary scan. Primary resolution: 70,000; Secondary resolution: 17,500. Scan range 100–1500 m / z; The collision energy gradients are 20 eV, 40 eV, and 60 eV.

6. The detection method according to claim 1, characterized in that, For quantitative analysis, the ZORBAX Eclipse Plus C18 chromatographic model is selected from at least one of the following: 5 μm, 4.6*250 mm, 5 μm, 4.6*150 mm, 5 μm, 4.6*100 mm, 5 μm, 4.6*50 mm, 5 μm, 2.1*100 mm, and 5 μm, 2.1*50 mm.

7. The detection method according to claim 1, characterized in that, For quantitative analysis, chromatographic conditions include: Detection wavelength: 280 nm; Column temperature 28–32℃; Flow rate: 0.8–1.2 mL / min; Injection volume: 8–12 μL.

8. The detection method according to claim 1, characterized in that, The quantitative analysis involves plotting a standard curve using the peak area and concentration of standard working solutions at different concentrations as parameters. Then, by substituting the peak area corresponding to the active ingredient in the sample under the same chromatographic conditions into the standard curve, the content of each component to be tested in Chinese whisky can be obtained.

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