Production equipment and preparation method of cordyceps militaris

Abstract

The invention belongs to the technical field of cordyceps militaris production and processing, and particularly relates to a cordyceps militaris preparation method which comprises the following steps: raw material pretreatment, culture medium preparation, strain inoculation, segmented culture, intermediate regulation and control, maturity judgment, harvesting, drying and crushing, and the steps are sequentially linked to realize large-scale preparation of cordyceps militaris. The method has the beneficial effects that the problems of fuzzy parameters and non-standard operation in the prior art are solved, the parameters in each step are quantized and controllable, the product batch difference rate is reduced, and the quality stability is improved; by adopting the collaborative design of segmented culture and intermediate regulation, the deformity rate of the sporocarp can be reduced, the yield is improved compared with that of a traditional method, and the culture period can be shortened.

Description

Technical Field

[0001] This application belongs to the field of Cordyceps militaris production and processing technology, and in particular relates to a Cordyceps militaris production equipment and preparation method. Background Technology

[0002] Cordyceps militaris, a fungus with both medicinal and edible value, contains active ingredients such as cordycepic acid and cordycepin, which have significant health benefits. Market demand continues to grow, making large-scale, standardized production a core requirement for the industry's development. Currently, artificial preparation techniques for Cordyceps militaris have been applied to some extent, but many technical problems still need to be solved in actual production, resulting in unstable product quality, low yield, and difficulty in improving production efficiency.

[0003] In existing technologies, the raw material pretreatment process lacks precise parameter control, often resulting in incomplete or excessive sterilization of pupae. The temperature and time parameters for mother culture activation are vague, easily leading to insufficient strain activity or contamination by other microorganisms. Culture medium preparation relies heavily on empirical ratios, with a lack of scientific basis for controlling the carbon-to-nitrogen ratio, water content, and pH, resulting in nutrient imbalances that directly affect mycelial colonization and fruiting body differentiation. Inoculation processes often employ smearing or pouring methods, which not only compromise sterility but also make precise control of the inoculation amount difficult, leading to inconsistent germination rates and prolonged culture periods.

[0004] The cultivation stage commonly employs a "one-size-fits-all" control model, failing to tailor temperature and light regulation to the specific growth characteristics of mycelium and fruiting bodies. Poor coordination between light intensity, light cycle, and temperature leads to high rates of deformed fruiting bodies and low uniformity of growth. During intermediate control, the linkage between humidity and ventilation is insufficient, often relying on intermittent manual adjustments, making it difficult to maintain a stable growth environment and easily resulting in excessive mycelial growth or fruiting body wilting. Maturity assessment primarily depends on manual observation, lacking clear quantitative standards. Harvesting too early or too late can cause fluctuations in the content of active ingredients, affecting product quality.

[0005] The drying process often employs high-temperature rapid drying, which easily damages the heat-sensitive active ingredients in Cordyceps militaris. The pulverization process lacks a grading and screening mechanism, resulting in uneven particle size in the finished product, affecting subsequent processing and use. Correspondingly, the production equipment is mostly decentralized, single-function devices with poor integration between stages. It lacks integrated temperature, humidity, light, and gas sensors and automatic control modules, resulting in low automation and difficulty in achieving precise control in large-scale production. This leads to significant batch-to-batch quality variations, hindering the high-quality development of the Cordyceps militaris industry, and requires improvement. Summary of the Invention

[0006] The purpose of this application is to provide a holy grail for the production of Cordyceps militaris and a preparation method that can solve the above-mentioned problems.

[0007] The purpose of this application is to provide a method for preparing Cordyceps militaris, including the following steps: raw material pretreatment, culture medium preparation, inoculation of fungi, segmented culture, intermediate regulation, maturity determination, harvesting, drying and pulverization, with each step sequentially connected to achieve large-scale preparation of Cordyceps militaris.

[0008] Furthermore, the raw material pretreatment includes activation of Cordyceps militaris mother culture and treatment of pupae. The activation temperature of the mother culture is controlled at 25-28℃, and the activation time is 48-72h. The pupae are washed, sterilized, and drained. The sterilization conditions are 121℃ and 0.1MPa for 20-30min.

[0009] The mother culture is activated and the pupae are treated using a raw material pretreatment device. The mother culture is cultured in an activation chamber at 25-28℃ for 48-72 hours, which can effectively ensure and improve the germination rate of the strain. After the pupae are cleaned by the cleaning module in the device to remove impurities, they are sent to the high-pressure sterilization unit and sterilized at 121℃ and 0.1MPa for 20-30 minutes. This ensures thorough sterilization and avoids over-sterilization that could destroy the nutrients in the pupae, effectively solving the problems of incomplete sterilization or insufficient strain activity.

[0010] Furthermore: the culture medium is prepared using rice and wheat as carbon sources, silkworm pupa powder as nitrogen source, and glucose, potassium dihydrogen phosphate and magnesium sulfate are added. The carbon-nitrogen ratio is adjusted to 20-25:1, the water content of the culture medium is controlled to 60-65%, and the pH value is adjusted to 5.5-6.5.

[0011] The culture medium is prepared in a tank using rice and wheat as a composite carbon source at a mass ratio of 1:1, silkworm pupa powder as a nitrogen source, and glucose (2-3%), potassium dihydrogen phosphate (0.1-0.2%), and magnesium sulfate (0.05-0.1%). The carbon-nitrogen ratio is precisely controlled to 20-25:1, the water content to 60-65%, and the pH value to 5.5-6.5 by the pH adjustment module in the tank. This meets the nutritional requirements for Cordyceps militaris growth, avoids the nutritional imbalance caused by empirical formulation, and improves the mycelial colonization rate.

[0012] Furthermore, the inoculation of the strain adopts aseptic spray inoculation method, the inoculation amount is 5-8% of the culture medium mass, the cleanliness of the inoculation environment is not lower than Class 10000, and the inoculation temperature is maintained at 22-25℃.

[0013] The prepared culture medium was transferred to a sterile inoculation room, which was maintained at a cleanliness level of 10,000. A spray inoculation module was used to spray the bacterial culture solution at 5-8% of the culture medium mass, and the inoculation temperature was controlled at 22-25℃. Compared to traditional smear or pour inoculation methods, this method offers superior sterility, smaller inoculation volume errors, ensures consistent bacterial germination rates, and shortens the culture cycle.

[0014] Furthermore, the segmented cultivation includes a mycelial cultivation stage and a fruiting body cultivation stage; the temperature of the mycelial cultivation stage is 20-22℃, and the cultivation is carried out in the dark for 7-10 days; the temperature of the fruiting body cultivation stage is raised to 23-25℃, and the cultivation is carried out under light.

[0015] Furthermore, the illumination during the fruiting body cultivation stage is alternating illumination, with a light intensity of 300-500 lx, a light duration of 10-12 h / d, a dark duration of 12-14 h / d, and a cultivation cycle of 18-22 days.

[0016] The inoculated culture medium is then transferred to a segmented incubator, which incorporates temperature and humidity sensors and a light control module to achieve precise two-stage control. Mycelial stage: Culture at 20-22℃ in a dark environment for 7-10 days to promote rapid mycelial spread and colonization; Fruiting body stage: The temperature is raised to 23-25℃, and alternating light of 300-500 lx is provided through the light regulation module for 18-22 days. This method can meet the physiological characteristics of Cordyceps militaris at different growth stages, effectively reduce the deformity rate of fruiting bodies and ensure uniform growth, thereby avoiding the uneven growth problem caused by "one-size-fits-all" regulation.

[0017] Furthermore, the intermediate control includes humidity control and ventilation control. During the mycelial culture stage, the ambient humidity is maintained at 65-70%, and during the fruiting body culture stage, the humidity is increased to 80-85%. Ventilation is carried out once every 8-12 hours throughout the process, with each ventilation lasting 15-20 minutes, and sterile air circulation is maintained during ventilation.

[0018] The ventilation components and humidity control module of the segmented incubator are linked to ensure a stable growth environment in real time, preventing mycelial overgrowth or fruiting body withering, and ensuring and improving the survival rate of fruiting bodies.

[0019] Furthermore, the maturity criteria are that the fruiting body reaches a height of 5-8cm, is orange-yellow or orange-red in color, and the top of the stroma is swollen and without deformity. At this point, cultivation is terminated and the harvesting process begins.

[0020] Based on quantitative standards, specifically the fruiting body height of 5-8cm, orange-yellow / reddish-orange color, and swollen and deformed top of the stroma, maturity is confirmed by manual observation and equipment size detection module to ensure accurate harvesting timing and stable content of effective components.

[0021] Furthermore, after harvesting, the product is first pre-dried at a low temperature of 35-40℃ for 2-3 hours, then dried at 50-55℃ until the moisture content is ≤8%, and finally pulverized through a 100-120 mesh sieve to obtain the finished Cordyceps militaris powder.

[0022] After the fruiting bodies are harvested without damage using a harvesting device, they are sent to a segmented low-temperature dryer. They are first pre-dried at 35-40℃ for 2-3 hours to remove surface moisture, and then dried at 50-55℃ until the moisture content is ≤8%. Compared with traditional high-temperature drying, the retention rate of heat-sensitive active ingredients is increased by 15-20%. At the same time, the dried Cordyceps militaris is pulverized by a pulverizing device equipped with a 100-120 mesh grading screen, resulting in a uniform particle size that meets the requirements for subsequent processing.

[0023] Furthermore, this application also provides a production equipment for preparing Cordyceps militaris, characterized in that it includes a raw material pretreatment device, a culture medium preparation tank, a sterile inoculation room, a segmented incubator, a harvesting device, a drying device, and a pulverizing device connected in sequence. The segmented incubator has a built-in temperature sensor, humidity sensor, light control module, and ventilation components. The drying equipment is a segmented low-temperature dryer, and the pulverizing device is equipped with a grading screen.

[0024] The specific process is as follows, and each device is connected sequentially through a sterile transport channel: 1. Start the raw material pretreatment device: Introduce the Cordyceps militaris mother culture into the activation culture module, set the temperature to 25-28℃ and the time to 48-72h for activation, and at the same time put the pupae into the cleaning module. After cleaning, transfer them to the sterilization unit and set the sterilization parameters to 121℃, 0.1MPa and 20-30min. After sterilization, drain and set aside for later use. 2. Start the culture medium preparation tank: Add rice, wheat, silkworm pupa powder and additives according to the ratio, inject deionized water, mix evenly through the stirring module in the tank, and adjust to a carbon-nitrogen ratio of 20-25:1, water content of 60-65%, and pH value of 5.5-6.5 using a pH sensor and moisture detection module. After preparation, transport it to the sterile inoculation room through a sterile pipeline. 3. Aseptic inoculation room operation: Turn on the cleanliness control system and maintain it at Class 10,000 and the temperature control module to keep the temperature at 22-25℃. Inject the activated bacterial culture into the spray inoculation module, set the inoculation amount to 5-8% of the culture medium mass, and spray the culture medium evenly for inoculation. 4. Segmented incubator operation: After inoculation, the culture medium is delivered into the segmented incubator through a sterile delivery device. First, set the mycelial culture parameters, specifically (20-22℃, dark, humidity 65-70%, aeration 20min every 12h). After running for 7-10 days, automatically switch to the fruiting body culture parameters, specifically (23-25℃, 300-500lx alternating light, humidity 80-85%, aeration 15min every 8h), and continue culturing for 18-22 days. 5. Maturity determination and harvesting: After confirming that the fruiting bodies have reached the maturity standard by using the observation window of the incubator and the size detection module, the harvesting device is started to collect the fruiting bodies non-destructively and transport them to the drying equipment. 6. Drying and pulverizing: Start the segmented low-temperature dryer, set the first stage to 35-40℃ for 2-3 hours, and the second stage to 50-55℃ until the moisture content is ≤8%; after drying, transfer the product to the pulverizing device, turn on the grading screen, pulverize and collect the Cordyceps militaris dry powder product to complete the large-scale preparation.

[0025] The beneficial effects of this application are: 1. It solves the problems of ambiguous parameters and non-standard operation in the existing technology, making the parameters of each step quantifiable and controllable, reducing the batch variation rate of products, and improving the quality stability; 2. The synergistic design of segmented cultivation and intermediate regulation can reduce the deformity rate of fruiting bodies, increase yield compared with traditional methods, and shorten the cultivation cycle. 3. The equipment has a high degree of integration, enabling automated linkage from raw material processing to finished product processing, thereby reducing manual intervention, lowering the risk of pollution, and meeting the needs of large-scale production; 4. The raw materials for production and processing are widely available and the costs are controllable. The process flow is easy to replicate, making it suitable for large-scale industrial production. Attached Figure Description

[0026] Figure 1 This is a flowchart of the application. Detailed Implementation

[0027] The technical solutions of the embodiments of this application will be clearly described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application are within the scope of protection of this application.

[0028] The terms "first," "second," etc., used in the specification and claims of this application are used to distinguish similar objects and not to describe a specific order or sequence. It should be understood that such use of data can be interchanged where appropriate so that embodiments of this application can be implemented in orders other than those illustrated or described herein, and the objects distinguished by "first," "second," etc., are generally of the same class and the number of objects is not limited; for example, a first object can be one or more. Furthermore, in the specification and claims, "and / or" indicates at least one of the connected objects, and the character " / " generally indicates that the preceding and following objects are in an "or" relationship.

[0029] The production equipment and preparation method of Cordyceps militaris provided in this application will be described in detail below with reference to the accompanying drawings and through specific embodiments and application scenarios.

[0030] In this application, "Cordyceps militaris," also known as North Cordyceps, is the type species of the genus Cordyceps, belonging to the phylum Ascomycota, order Hypocreales, family Clavicipitaceae. The stroma grows singly or in groups from the head or segments of the parasitic pupa, and is orange-yellow or orange-red in color, generally 2–8 cm in length. The mother culture of Cordyceps militaris can be a purified and rejuvenated strain, derived from strains preserved at the China General Microbiological Culture Collection Center, or a commercially available qualified mother culture of Cordyceps militaris. After treatment with the activation process described in this invention, the activity and purity of the strain are ensured.

[0031] Meanwhile, the carbon source is selected from commercially available high-quality rice and wheat (ensuring a moisture content of ≤12%, free from mold and impurities); the nitrogen source is selected from food-grade silkworm pupa powder; glucose, potassium dihydrogen phosphate, and magnesium sulfate are all analytical grade reagents; and the pupae are selected from fresh, undamaged domestic silkworm pupae or tussah silkworm pupae, which can be purchased from large-scale silkworm breeding bases.

[0032] Example 1: Preparation of Basic Optimized Cordyceps militaris I. Preparation 1. Materials: Purified and rejuvenated Cordyceps militaris mother seed, silkworm pupae (from large-scale silkworm breeding bases), rice, wheat, food-grade silkworm pupa powder, glucose, potassium dihydrogen phosphate, and magnesium sulfate (all analytical grade).

[0033] 2. Equipment: The production equipment described in claim 10 of this invention includes a raw material pretreatment device, a culture medium preparation tank, a 10,000-level sterile inoculation room, a segmented incubator with temperature, humidity, light and gas sensors, a non-destructive harvesting device, a segmented low-temperature dryer, and a 110-mesh grading and pulverizing device.

[0034] II. Preparation Process 1. Raw material pretreatment: The mother culture is introduced into the activation module of the raw material pretreatment device, the temperature is set to 26℃, and the activation time is 48h; after the pupae are cleaned by the device's cleaning module to remove impurities, they are transferred to the high-pressure sterilization unit, set to 121℃, 0.1MPa, and sterilized for 25min. After sterilization, they are drained and ready for use. 2. Culture medium preparation: Add rice and wheat in a 1:1 mass ratio to the culture medium preparation tank, add silkworm pupa powder, supplement with 2.5% glucose, 0.15% potassium dihydrogen phosphate, and 0.08% magnesium sulfate, add deionized water, stir evenly, and adjust the carbon-nitrogen ratio to 22:1, water content to 62%, and pH value to 6.0. After preparation, transport it to the sterile inoculation room through sterile pipeline. 3. Inoculation: Turn on the cleanliness control system of the sterile inoculation room, adjust the temperature to 23℃, inject the activated inoculation solution into the spray inoculation module, set the inoculation amount to 6% of the culture medium mass, and spray the culture medium evenly for inoculation. 4. Segmented culture: The inoculated culture medium is sent to a segmented incubator. The first stage is mycelial culture, with a temperature of 21℃, darkness, and humidity of 68%, and ventilation for 18 minutes every 10 hours, for 8 days. The second stage is fruiting body culture, with automatic parameter switching: temperature 24℃, light intensity 400lx, alternating light for 11 hours / day and darkness for 13 hours / day, humidity 82%, and ventilation for 18 minutes every 10 hours, for 20 days.

[0035] 5. Maturity determination: By using the observation window of the incubator and the size detection module, the fruiting body is determined to be mature if it is 6-7cm in height, orange-yellow in color, and the top of the stroma is swollen without deformity. 6. Harvesting, drying and crushing: Start the harvesting device to collect the fruiting bodies without damage, and send them into a segmented low temperature dryer. In the first stage, pre-dry at 38℃ for 2.5 hours, and in the second stage, dry at 52℃ until the moisture content is 7%. After drying, transfer the fruiting bodies to the crushing device and crush them through a 110-mesh grading screen to collect the finished Cordyceps militaris powder.

[0036] III. Test Results Finished product testing: fruiting body deformity rate 3.2%, batch difference rate 2.1%; cordycepic acid content 7.8%, cordycepin content 0.18%; compared with traditional methods, the yield increased by 22%, the cultivation cycle was shortened by 4 days, the contamination rate of miscellaneous bacteria was 0.3%, and the retention rate of active ingredients was 17% higher than that of traditional high-temperature dried products.

[0037] Example 2, Preparation of high-yield Cordyceps militaris I. Material Preparation 1. Materials: Cordyceps militaris mother strain was a commercially available qualified strain, tussah silkworm pupae, rice, wheat, food-grade silkworm pupa powder, glucose, potassium dihydrogen phosphate, and magnesium sulfate were all of analytical grade.

[0038] 2. Equipment: Same as the production equipment described in Example 1.

[0039] II. Preparation Process 1. Raw material pretreatment: The mother culture is activated in the raw material pretreatment device at a temperature of 28℃ for 72 hours; after cleaning, the pupae are transferred to the sterilization unit at 121℃ and 0.1MPa for 30 minutes, and then drained for later use. 2. Culture medium preparation: Rice and wheat are mixed in a 1:1 mass ratio in a culture medium preparation tank. A high proportion of silkworm pupa powder is added, and 3% glucose, 0.2% potassium dihydrogen phosphate, and 0.1% magnesium sulfate are added. The carbon-nitrogen ratio is adjusted to 25:1, the water content to 65%, and the pH to 6.5. After stirring evenly, the mixture is aseptically transported to the inoculation room. 3. Inoculation: The temperature of the sterile inoculation room is 25℃, the cleanliness level is 10000, the spray inoculation amount is 8%, and the inoculation solution is sprayed evenly. 4. Segmented cultivation: Mycelial stage: temperature 22℃, dark, humidity 70%, aeration 20min every 8h, cultivation for 10d; Fruiting body stage: temperature 25℃, light intensity 500lx, alternating light 12h / d, darkness 12h / d, humidity 85%, aeration 20min every 8h, cultivation for 22d.

[0040] 5. Maturity judgment: The fruiting body is judged to be mature when it is 7-8cm in height, orange-red in color, and the top of the stroma is swollen without deformity; 6. Harvesting, drying and crushing: After non-destructive harvesting, pre-dry at 39℃ for 3 hours in a segmented low-temperature dryer, and dry at 55℃ until the moisture content is 6%; crush through a 120-mesh grading screen and collect the finished product.

[0041] III. Test Results Finished product testing: fruiting body deformity rate 2.8%, batch difference rate 2.3%; cordycepic acid content 8.2%, cordycepin content 0.20%; yield increased by 30% compared to traditional methods, culture cycle shortened by 3 days, contamination rate of miscellaneous bacteria 0.4%, and active ingredient retention rate increased by 20% compared to traditional high-temperature dried products, suitable for high-end markets with high requirements for active ingredient content.

[0042] Example 3: Preparation of Stable Cordyceps militaris I. Materials and Equipment 1. Materials: Cordyceps militaris, silkworm pupae, rice, wheat, food-grade silkworm pupa powder, glucose, potassium dihydrogen phosphate, and magnesium sulfate were all of analytical grade.

[0043] 2. Equipment: Same as the production equipment described in Example 1.

[0044] II. Preparation Process 1. Raw material pretreatment: The mother culture is activated at 25℃ for 60 hours in the activation module; the pupae are cleaned and sterilized for 20 minutes, then drained and set aside. 2. Culture medium preparation: Rice and wheat are mixed in a 1:1 ratio in the culture medium preparation tank, supplemented with 2% glucose, 0.1% potassium dihydrogen phosphate, and 0.05% magnesium sulfate, and the carbon-to-nitrogen ratio is adjusted to 20:1, the water content to 60%, and the pH to 5.5. The culture medium is then aseptically transported to the inoculation room. 3. Inoculation: The temperature of the sterile inoculation room is 22℃, the cleanliness level is 10000, the spray inoculation amount is 5%, and the spray is evenly applied; 4. Segmented cultivation: Mycelial stage: temperature 20℃, dark, humidity 65%, aeration 15min every 12h, cultivation for 7d; Fruiting body stage: temperature 23℃, light intensity 300lx, alternating light 10h / d, darkness 14h / d, humidity 80%, aeration 15min every 12h, cultivation for 18d.

[0045] 5. Maturity judgment: The fruiting body is judged to be mature when it is 5-6cm in height, orange-yellow in color, and the top of the stroma is swollen without deformity.

[0046] 6. Harvesting, drying and crushing: After non-destructive harvesting, pre-dry at 35℃ for 2 hours in a segmented low-temperature dryer, and dry at 50℃ until the moisture content is 8%; crush through a 100-mesh grading screen and collect the finished product.

[0047] III. Test Results Finished product testing: fruiting body deformity rate 4.5%, batch difference rate 2.8%; cordycepic acid content 7.1%, cordycepin content 0.16%; yield increased by 20% compared to traditional methods, culture cycle shortened by 5 days, contamination rate of miscellaneous bacteria 0.5%, and active ingredient retention rate increased by 15% compared to traditional high-temperature dried products; at the same time, the energy consumption of this embodiment is reduced by 18% compared to Example 1, making it suitable for large-scale energy-saving production scenarios, and the product quality meets industry standard requirements.

[0048] It should be noted that, in this document, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes that element. Furthermore, it should be noted that the scope of the methods and apparatuses in the embodiments of this application is not limited to performing functions in the order shown or discussed, but may also include performing functions substantially simultaneously or in the reverse order, depending on the functions involved. For example, the described methods may be performed in a different order than described, and various steps may be added, omitted, or combined. Additionally, features described with reference to certain examples may be combined in other examples.

[0049] The embodiments of this application have been described above with reference to the accompanying drawings. However, this application is not limited to the specific embodiments described above. The specific embodiments described above are merely illustrative and not restrictive. Those skilled in the art can make many other forms under the guidance of this application without departing from the spirit and scope of the claims, and all of these forms are within the protection scope of this application.

Claims

1. A method for preparing Cordyceps militaris, characterized by: The method comprises the following steps: The raw material pretreatment, medium preparation, strain inoculation, segmented cultivation, intermediate regulation, maturity determination, harvesting, drying and crushing are sequentially connected to realize the large-scale preparation of Cordyceps militaris.

2. The method of claim 1, wherein the Cordyceps militaris is prepared by the steps of: The raw material pretreatment comprises Cordyceps militaris mother strain activation and pupa treatment, the activation temperature is controlled to be 25-28℃, and the activation time is 48-72h; the pupa is drained after being washed and sterilized, and the sterilization condition is 121℃, 0.1MPa for 20-30min. ​ 3. The method of claim 1, wherein the Cordyceps militaris is prepared by the steps of: The medium preparation uses rice and wheat as carbon sources, and silkworm pupa powder as nitrogen source, and adds glucose, potassium dihydrogen phosphate and magnesium sulfate, the carbon-nitrogen ratio is adjusted to be 20-25:1, the water content of the medium is controlled to be 60-65%, and the pH value is adjusted to be 5.5-6.

5. ​ 4. The method of claim 1, wherein the Cordyceps militaris is prepared by the steps of: The strain inoculation adopts sterile spray inoculation, the inoculation amount is 5-8% of the medium mass, the inoculation environment cleanliness is not less than 10000 level, and the inoculation temperature is kept at 22-25℃. ​ 5. The method of claim 1, wherein the Cordyceps militaris is prepared by the steps of: The segmented cultivation comprises mycelium cultivation stage and fruiting body cultivation stage; the temperature of the mycelium cultivation stage is 20-22℃, and the light cultivation is kept for 7-10d; the temperature of the fruiting body cultivation stage is increased to 23-25℃, and the light cultivation is started. ​ 6. The method of claim 5, wherein the Cordyceps militaris is prepared by the steps of: The light of the fruiting body cultivation stage is alternating light, the light intensity is 300-500lx, the light time is 10-12h / d, the dark time is 12-14h / d, and the cultivation cycle is 18-22d.

7. The method of claim 1, wherein the Cordyceps militaris is prepared by the steps of: The intermediate regulation comprises humidity regulation and ventilation regulation, the environmental humidity of the mycelium cultivation stage is kept at 65-70%, the humidity of the fruiting body cultivation stage is increased to 80-85%, the ventilation is kept every 8-12h, and the ventilation time is 15-20min each time. ​ 8. The method of claim 1, wherein the Cordyceps militaris is prepared by the steps of: The maturity determination standard is that the height of the fruiting body reaches 5-8cm, the color is orange yellow or orange red, the stipe top is swollen and has no deformity, at this time, the cultivation is terminated and the harvesting step is entered. ​ 9. The method of claim 1, wherein the Cordyceps militaris is prepared by the steps of: After the harvesting, the fruiting body is pre-dried at 35-40℃ for 2-3h, then dried at 50-55℃ until the water content is ≤8%, and finally crushed through a 100-120 mesh screen to obtain Cordyceps militaris dry powder product. ​ 10. A production apparatus for the preparation method of the Cordyceps militaris according to any one of claims 1 to 9, characterized by: The method comprises sequentially connected raw material pretreatment device, medium preparation tank, sterile inoculation room, segmented cultivation box, harvesting device, drying equipment and crushing device; The segmented cultivation box is internally provided with temperature sensor, humidity sensor, light adjustment module and ventilation assembly; The drying equipment is a segmented low-temperature dryer, and the crushing device is provided with a grading screen.

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