Self-powered microsphere for promoting wound healing, preparation method and application

Abstract

The invention discloses a self-powered microsphere for promoting wound healing, a preparation method and application, and belongs to the field of functional composite materials. The preparation method comprises the following steps: firstly, ultrasonically crushing liquid metal in an aqueous solution of sodium vinyl benzene sulfonate, and carrying out suction filtration and drying to obtain liquid metal / sodium polyvinyl benzene sulfonate core-shell microspheres; dispersing in absolute ethyl alcohol, adding benzyl acrylate and an initiator under the protection of nitrogen, and fully mixing and stirring to react; then adding a fluorine-containing acrylic monomer, divinylbenzene and an initiator, continuously stirring, and carrying out a reaction system under the protection of nitrogen; performing suction filtration and drying to obtain multi-layer coated liquid metal core-shell particles; then dispersing in deionized water, adding sodium hydroxide to adjust the pH value of the dispersion liquid, and continuously stirring and etching; and finally, filling the dispersion liquid into a dialysis bag for dialysis, and drying to obtain the self-powered microspheres for promoting wound healing. The self-powered microsphere for promoting wound healing can be used in the fields of powder microsphere materials for promoting skin repair and medical materials for repairing other tissues.

Description

Technical Field

[0001] This invention belongs to the field of functional composite materials, specifically relating to a self-powered microsphere that promotes wound healing, its preparation method, and its application. Background Technology

[0002] As the largest organ in the human body, the skin performs many important functions. It serves not only as a physical barrier between the body and the external environment, effectively preventing the invasion of physical injuries (such as abrasions and collisions), chemical substances (such as toxic substances, acids, and alkalis), and pathogenic microorganisms (such as bacteria and viruses), but also plays multiple roles in sensation, metabolism, and thermoregulation. In daily life, skin injuries are unavoidable, and their potential harm can lead to various adverse reactions in the body. Not only does it remove the protective barrier, increasing the risk of infection, but long-term scarring and functional impairment can cause psychological and physiological damage that can lead to long-term distress for patients. Therefore, research on promoting wound healing is an important topic for improving health and quality of life.

[0003] Currently, electrostimulation therapy, as a safe and effective physical therapy method, has attracted the attention of researchers. It can promote cell proliferation, alleviate inflammation, and promote collagen growth by improving electrical signals at the wound site, thereby promoting wound healing. Self-powered materials, which can generate electricity through natural human movement or physiological processes, are widely used in the preparation of wound-healing dressings. However, current self-powered healing materials are dependent on material area and can easily cause wound compression, limiting their effectiveness in treating irregular wounds such as cuts and punctures. Furthermore, due to the limited volume of the material, it is difficult to personalize the material according to the wound condition, resulting in poor flexibility.

[0004] The paper *Advanced Functional Materials*, 2025, 35 (29), 2422188, describes the fabrication of a multilayer self-powered dressing using a bilayer structure mimicking the water management strategies of trees. The dressing's lower layer is prepared from an asymmetric electrospun film of polyacrylonitrile-graphene oxide and polycaprolactone, and silver and carbon nanotube electrodes are screen-printed to achieve unidirectional rapid discharge, providing an electrical stimulation signal. However, this research is limited by the three-dimensional structure requirements of the multilayer film; the dressing can only adhere to the wound surface for treatment and cannot adapt to more complex wound conditions such as lacerations. Furthermore, long-term use can easily cause wound compression and irritation. The paper *Advanced Materials*, 2023, 35 (16), 2208395, describes the development of a negative pressure integrated self-powered dressing by combining a triboelectric nanogenerator with negative pressure wound therapy technology. This dressing utilizes the mutual friction of its multilayer structure to convert mechanical energy into electrical energy, and applies electrical signals to the wound surface through a rectifier bridge and flexible electrodes, thereby reshaping the endogenous electric field of the wound and achieving bioelectric field therapy. However, the material's complex multi-layered structure makes it difficult to provide personalized treatment based on different wound conditions, and it is also difficult to adjust the dosage according to the wound condition. In addition, its portability and flexibility of use are also poor.

[0005] Powdered therapeutic materials offer greater flexibility than film dressings, facilitating personalized dosage adjustments for different wounds and enabling rapid compounding with other therapeutic agents in complex clinical settings. Furthermore, the superior adaptability of powdered materials to irregular wounds, coupled with the dimensional advantage of zero-dimensional materials, allows for better wound adhesion when treating irregular, deep wounds, reducing unnecessary pressure on sensitive wound surfaces. Currently, no research reports have been found on self-powered powdered materials. Therefore, the development of microsphere medical materials with good biocompatibility and self-generating capabilities to promote wound healing is urgently needed. Summary of the Invention

[0006] To address the problems in existing technologies, this invention provides a self-powered microsphere for promoting wound healing, its preparation method, and its application in composite materials. The self-powered microsphere for promoting wound healing obtained by this invention exhibits good biocompatibility and self-generating power, making it suitable for complex and irregular wound conditions. It can reduce unnecessary pressure on sensitive wound surfaces, and helps promote wound healing by reducing wound inflammation, promoting collagen and hair follicles, and inhibiting scar formation.

[0007] Specific technical solution of the present invention:

[0008] In a first aspect, a method for preparing self-powered microspheres that promote wound healing is provided, comprising the following steps:

[0009] (1) Liquid metal was ultrasonically pulverized in an aqueous solution of sodium vinylbenzenesulfonate, and liquid metal / sodium vinylbenzenesulfonate core-shell microspheres were obtained by filtration and drying;

[0010] (2) Disperse the liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres described in step (1) in anhydrous ethanol, add benzoyl acrylate and initiator, mix thoroughly and stir to react, and the reaction system is carried out under nitrogen protection; then, add fluorinated acrylic monomer, divinylbenzene and initiator, stir continuously, and the reaction system is carried out under nitrogen protection; finally, filter and dry to obtain multilayer coated liquid metal core-shell particles.

[0011] (3) Disperse the multilayer coated liquid metal core-shell particles obtained in step (2) in deionized water, adjust the pH of the dispersion to 10-11, and continuously stir and etch at room temperature; then, put the dispersion into a dialysis bag for dialysis; finally, dry to obtain self-powered microspheres that promote wound healing.

[0012] Furthermore, based on the weight of liquid metal per 100 parts by weight, sodium vinylbenzenesulfonate is 10-100 parts by weight, methyl methacrylate is 100-400 parts by weight, fluorinated acrylic acid monomer is 20-200 parts by weight, divinylbenzene is 100-1000 parts by weight, the initiator added each time is 2-15 parts by weight, and anhydrous ethanol is 20,000-100,000 parts by weight.

[0013] Furthermore, in step (1), the liquid metal is one of the gallium-indium alloys with a melting point of -19°C to 25°C;

[0014] Furthermore, in step (2), the fluorinated acrylic monomer is one of 2-(trifluoromethyl)acrylic acid, trifluoroethyl methacrylate, and hexafluorobutyl methacrylate; in step (2), the initiator is one of azobisisobutyronitrile, azobisisoheptanenitrile, and benzoyl peroxide; in step (2), the reaction time of the liquid metal / sodium polyvinylbenzenesulfonate core-shell microsphere dispersion, methyl methacrylate, and initiator is 3-10 h, and the temperature is 50-90 °C; in step (2), after adding the fluorinated acrylic monomer, divinylbenzene, and initiator, the reaction time is 3-10 h, and the temperature is 50-90 °C.

[0015] Furthermore, in step (2), the pH of the dispersion is adjusted to 10-11 using sodium hydroxide or potassium hydroxide.

[0016] Furthermore, in step (3), the content of the multilayer coated liquid metal core-shell particles in deionized water is 0.01-0.1 g / mL, the etching time is 8-24 h, and the dialysis time is 3-5 days.

[0017] Furthermore, the particle size of the self-powered wound-healing microspheres is 50-1500 nm.

[0018] A self-powered microsphere for promoting wound healing is used to prepare medical materials that promote wound healing. Specifically, the medical material is prepared by mixing the self-powered microsphere for promoting wound healing with a topical ointment or liquid drug to form a self-powered healing-promoting composite material.

[0019] In use, the self-powered wound-healing microspheres are applied directly to the skin injury site, or the self-powered wound-healing microspheres are mixed with topical ointments or liquid drugs to prepare a self-powered healing composite material which is then applied or sprayed onto the wound.

[0020] Furthermore, the topical ointment is one or more of erythromycin ointment, Jingwanhong ointment, mupirocin ointment, and recombinant human epidermal growth factor gel; the liquid drug is one or more of iodine tincture, Kangfuxin liquid, and Beifuji spray.

[0021] Furthermore, the liquid metal inside the self-powered wound-healing microsphere has good deformation capability and drivability. Skin shaking or body mechanical movement can drive the liquid metal to oscillate and generate electricity through friction with the outer shell.

[0022] Furthermore, the self-powered wound-healing microspheres are non-cytotoxic and can promote collagen and hair follicle regeneration and reduce wound inflammation by improving the electrical signal at the wound site. This helps to promote wound healing and reduce scar formation, and can be widely used in the preparation of medical materials that promote wound healing.

[0023] This invention has at least the following beneficial effects:

[0024] (1) In step (1) of the preparation method of the self-powered wound healing microsphere prepared by the present invention, the first layer is coated with sodium vinylbenzenesulfonate. Since the liquid metal can rapidly catalyze the free radical reaction under ultrasonic treatment, sodium vinylbenzenesulfonate can be polymerized into sodium polyvinylbenzenesulfonate to coat the surface of the liquid metal, stabilize the liquid core, and the anions on its surface help it to be well dispersed in the polymerization, avoid a large amount of agglomeration, and ensure the uniformity of subsequent coating.

[0025] (2) In step (2) of the preparation method of the self-powered wound healing microsphere prepared by the present invention, benzoyl acrylate is polymerized on the surface of liquid metal / sodium polyvinylbenzenesulfonate core-shell microsphere to form a second layer. This layer can serve as an isolation layer, avoiding the situation where the inner layer is cross-linked and difficult to etch due to the infiltration of the outermost cross-linking agent divinylbenzene, thereby increasing the etchable space and improving the cavity volume of the self-powered wound healing microsphere after etching.

[0026] (3) In step (2) of the method for preparing the self-powered wound-healing microspheres of the present invention, the third layer coating with fluorinated acrylic monomer and divinylbenzene, by introducing fluorinated monomer, improves the electron-acquiring ability of the polymer shell, which helps to achieve triboelectric power generation with metal. Divinylbenzene enables the outermost layer to achieve chemical cross-linking, which can maintain structural stability in subsequent etching.

[0027] (4) In step (3) of the method for preparing the self-powered wound-healing microspheres of the present invention, the etching process can remove the uncrosslinked sodium polyvinylbenzenesulfonate and polymethyl methacrylate, thereby forming a cavity inside the microsphere. Since there is no chemical bond between the liquid metal and the outermost layer, the liquid metal regains its fluidity and can generate electricity through triboelectricity with the fluorine-containing shell when vibration is generated.

[0028] (5) The self-powered wound healing microspheres prepared by the present invention are micro-nano in size and can be evenly sprinkled or coated on wounds with irregular edges or difficult to cover with traditional dressings, which helps to treat complex wounds more comprehensively, more in detail and more effectively.

[0029] (6) The self-powered microspheres that promote wound healing prepared by the present invention can be used as powdered treatment materials. They can be used for personalized treatment of wounds in different situations with different dosages. At the same time, they can be quickly combined with other types of drugs to achieve synergistic treatment, which helps to adapt to complex clinical treatment situations such as pressure ulcers, burns, and post-radiotherapy wounds.

[0030] (7) The self-powered microspheres for promoting wound healing prepared by the present invention have excellent biocompatibility, are non-toxic, do not cause pressure on the wound surface, and do not cause secondary damage to sensitive skin.

[0031] (8) The self-powered microspheres for promoting wound healing prepared by the present invention have good self-power generation capability. The liquid properties of liquid metal give it high deformation capability and driveability. Everyday mechanical movements such as skin shaking or bending can cause the liquid metal to vibrate, thereby realizing frictional power generation with the outer shell, improving the electrical signal at the wound site, which helps to promote wound healing and inhibit scar hyperplasia. Attached Figure Description

[0032] Figure 1 A scanning electron microscope image of the self-powered microspheres that promote wound healing in Example 1;

[0033] Figure 2 This is a scanning electron microscope image of the self-powered wound-healing microspheres in Example 1 after ultrasonic fragmentation.

[0034] Figure 3 This is a transmission electron microscope image of the multilayer coated liquid metal core-shell particles in Example 1;

[0035] Figure 4 A transmission electron microscope image of the self-powered microspheres that promote wound healing in Example 1;

[0036] Figure 5 The image shows a fluorescence microscope photograph of fibroblasts co-cultured with the microsphere filtrate that promotes wound healing using self-powered technology in Example 1, after staining with Calcein-AM / PI live and dead cells (white indicates live cells).

[0037] Figure 6 The self-generating power test of the microspheres that promote wound healing with self-powered power generation in Example 1 under different pressures;

[0038] Figure 7 The image shows H&E staining of the healed wound site in Example 1 after 9 days. Detailed Implementation

[0039] Unless otherwise specified, all reagents used in the embodiments of this invention can be purchased commercially.

[0040] It should be understood that the specific embodiments described herein are for illustrative and explanatory purposes only and are not intended to limit the invention. In addition to the raw materials used in the embodiments of this invention, any raw material components that contain the same functional groups or include the same structural units involved in this invention, and which are replaced by equivalent substitutions, should be included within the scope of protection of this invention. The invention will be further described below with reference to specific embodiments.

[0041] The present invention provides accompanying drawings of detection results for some embodiments. Other embodiments and comparative examples use the same detection method. Those skilled in the art can directly and without doubt determine the content of the embodiments of the present invention using the detection method provided by the present invention.

[0042] The present invention will be further described below with reference to the embodiments.

[0043] Example 1

[0044] (1) Preparation of liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres: 100 parts by weight of liquid metal at 6℃ was ultrasonically pulverized in an aqueous solution of 50 parts by weight of sodium vinylbenzenesulfonate, and liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres were obtained by filtration and drying.

[0045] (2) Preparation of multilayer coated liquid metal core-shell particles: The liquid metal / sodium polyvinylbenzenesulfonate microspheres obtained in step (1) were dispersed in 50,000 parts by weight of anhydrous ethanol, and 200 parts by weight of methyl acrylate and 5 parts by weight of azobisisobutyronitrile were added and mixed thoroughly. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Then, 50 parts by weight of 2-(trifluoromethyl)acrylic acid, 500 parts by weight of divinylbenzene and 5 parts by weight of azobisisobutyronitrile were added. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Finally, the mixture was filtered and dried to obtain multilayer coated liquid metal core-shell particles.

[0046] (3) Preparation of self-powered microspheres for promoting wound healing: (3) The multi-layered liquid metal core-shell particles obtained in step (2) were dispersed in deionized water. The content of liquid metal core-shell particles in deionized water was 0.01 g / mL. Sodium hydroxide was added to adjust the pH of the dispersion to 10-11. The dispersion was stirred and etched at room temperature for 12 h. Then, the dispersion was placed in a dialysis bag and dialyzed for 4 days. Finally, the dispersion was dried to obtain self-powered microspheres for promoting wound healing.

[0047] (4) Application of self-powered microspheres for promoting wound healing: Take 0.5g of self-powered microspheres for promoting wound healing and sprinkle them evenly on a 4mm diameter wound on the back of a mouse. The wound recovery is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe hair follicle regeneration. The hair follicle activation is shown in Table 2.

[0048] Example 2

[0049] (1) Preparation of liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres: 100 parts by weight of liquid metal at -19℃ was ultrasonically pulverized in 10 parts by weight of sodium vinylbenzenesulfonate aqueous solution, and liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres were obtained by filtration and drying.

[0050] (2) Preparation of multilayer coated liquid metal core-shell particles: The liquid metal / sodium polyvinylbenzenesulfonate microspheres obtained in step (1) were dispersed in 20,000 parts by weight of anhydrous ethanol, and 100 parts by weight of benzyl acrylate and 2 parts by weight of azobisisoheptanenitrile were added and mixed thoroughly. The mixture was stirred continuously and heated to 50°C for 10 h. The reaction system was carried out under nitrogen protection. Then, 50 parts by weight of 2-(trifluoromethyl)acrylic acid, 500 parts by weight of divinylbenzene and 2 parts by weight of azobisisoheptanenitrile were added. The mixture was stirred continuously and heated to 50°C for 10 h. The reaction system was carried out under nitrogen protection. Finally, the mixture was filtered and dried to obtain multilayer coated liquid metal core-shell particles.

[0051] (3) Preparation of self-powered microspheres for promoting wound healing: (3) The multi-layered liquid metal core-shell particles obtained in step (2) were dispersed in deionized water. The content of liquid metal core-shell particles in deionized water was 0.01 g / mL. Potassium hydroxide was added to adjust the pH of the dispersion to 10-11. The dispersion was stirred and etched at room temperature for 8 hours. Then, the dispersion was placed in a dialysis bag and dialyzed for 3 days. Finally, the dispersion was dried to obtain self-powered microspheres for promoting wound healing.

[0052] (4) Application of self-powered microspheres for promoting wound healing: Take 0.5g of self-powered microspheres for promoting wound healing and sprinkle them evenly on a 4mm diameter wound on the back of a mouse. The wound recovery is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe hair follicle regeneration. The hair follicle activation is shown in Table 2.

[0053] Example 3

[0054] (1) Preparation of liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres: 100 parts by weight of liquid metal at 3℃ was ultrasonically pulverized in 100 parts by weight of sodium vinylbenzenesulfonate aqueous solution, and liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres were obtained by filtration and drying.

[0055] (2) Preparation of multilayer coated liquid metal core-shell particles: The liquid metal / sodium polyvinylbenzenesulfonate microspheres obtained in step (1) were dispersed in 100,000 parts by weight of anhydrous ethanol, and 400 parts by weight of methyl methacrylate and 15 parts by weight of azobisisobutyronitrile were added and mixed thoroughly. The mixture was stirred continuously and heated to 55°C for 7 h. The reaction was carried out under nitrogen protection. Then, 50 parts by weight of 2-(trifluoromethyl)acrylic acid, 500 parts by weight of divinylbenzene and 5 parts by weight of azobisisobutyronitrile were added. The mixture was stirred continuously and heated to 70°C for 6 h. The reaction was carried out under nitrogen protection. Finally, the mixture was filtered and dried to obtain multilayer coated liquid metal core-shell particles.

[0056] (3) Preparation of self-powered microspheres for promoting wound healing: (3) The multi-layered liquid metal core-shell particles obtained in step (2) were dispersed in deionized water. The content of liquid metal core-shell particles in deionized water was 0.05 g / mL. Sodium hydroxide was added to adjust the pH of the dispersion to 10-11. The dispersion was stirred and etched at room temperature for 24 h. Then, the dispersion was placed in a dialysis bag and dialyzed for 5 days. Finally, the dispersion was dried to obtain self-powered microspheres for promoting wound healing.

[0057] (4) Application of self-powered microspheres for promoting wound healing: Take 0.5g of self-powered microspheres for promoting wound healing and sprinkle them evenly on a 4mm diameter wound on the back of a mouse. The wound recovery is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe hair follicle regeneration. The hair follicle activation is shown in Table 2.

[0058] Example 4

[0059] (1) Preparation of liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres: 100 parts by weight of liquid metal at 16℃ was ultrasonically pulverized in an aqueous solution of 50 parts by weight of sodium vinylbenzenesulfonate, and liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres were obtained by filtration and drying.

[0060] (2) Preparation of multilayer coated liquid metal core-shell particles: The liquid metal / sodium polyvinylbenzenesulfonate microspheres obtained in step (1) were dispersed in 50,000 parts by weight of anhydrous ethanol, and 200 parts by weight of benzoyl acrylate and 5 parts by weight of benzoyl peroxide were added and mixed thoroughly. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Then, 100 parts by weight of trifluoroethyl methacrylate, 500 parts by weight of divinylbenzene and 10 parts by weight of benzoyl peroxide were added. The mixture was stirred continuously and heated to 80°C for 5 hours. The reaction was carried out under nitrogen protection. Finally, the mixture was filtered and dried to obtain multilayer coated liquid metal core-shell particles.

[0061] (3) Preparation of self-powered microspheres for promoting wound healing: (3) The multi-layered liquid metal core-shell particles obtained in step (2) were dispersed in deionized water. The content of liquid metal core-shell particles in deionized water was 0.1 g / mL. Sodium hydroxide was added to adjust the pH of the dispersion to 10-11. The dispersion was stirred and etched at room temperature for 8 hours. Then, the dispersion was placed in a dialysis bag and dialyzed for 4 days. Finally, the dispersion was dried to obtain self-powered microspheres for promoting wound healing.

[0062] (4) Application of self-powered microspheres for promoting wound healing: Take 0.5g of self-powered microspheres for promoting wound healing and sprinkle them evenly on a 4mm diameter wound on the back of a mouse. The wound recovery is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe hair follicle regeneration. The hair follicle activation is shown in Table 2.

[0063] Example 5

[0064] (1) Preparation of liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres: 100 parts by weight of liquid metal at 25°C was ultrasonically pulverized in an aqueous solution of 50 parts by weight of sodium vinylbenzenesulfonate, and liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres were obtained by filtration and drying.

[0065] (2) Preparation of multilayer coated liquid metal core-shell particles: The liquid metal / sodium polyvinylbenzenesulfonate microspheres obtained in step (1) were dispersed in 100,000 parts by weight of anhydrous ethanol, and 200 parts by weight of methyl methacrylate and 5 parts by weight of azobisisobutyronitrile were added and mixed thoroughly. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Then, 200 parts by weight of trifluoroethyl methacrylate, 1,000 parts by weight of divinylbenzene and 15 parts by weight of azobisisobutyronitrile were added. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Finally, the mixture was filtered and dried to obtain multilayer coated liquid metal core-shell particles.

[0066] (3) Preparation of self-powered microspheres for promoting wound healing: (3) The multi-layered liquid metal core-shell particles obtained in step (2) were dispersed in deionized water. The content of liquid metal core-shell particles in deionized water was 0.1 g / mL. Sodium hydroxide was added to adjust the pH of the dispersion to 10-11. The dispersion was stirred and etched at room temperature for 12 h. Then, the dispersion was placed in a dialysis bag and dialyzed for 5 days. Finally, the dispersion was dried to obtain self-powered microspheres for promoting wound healing.

[0067] (4) Application of self-powered microspheres for promoting wound healing: Take 0.5g of self-powered microspheres for promoting wound healing and sprinkle them evenly on a 4mm diameter wound on the back of a mouse. The wound recovery is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe hair follicle regeneration. The hair follicle activation is shown in Table 2.

[0068] Example 6

[0069] (1) Preparation of liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres: 100 parts by weight of liquid metal at 6℃ was ultrasonically pulverized in an aqueous solution of 50 parts by weight of sodium vinylbenzenesulfonate, and liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres were obtained by filtration and drying.

[0070] (2) Preparation of multilayer coated liquid metal core-shell particles: The liquid metal / sodium polyvinylbenzenesulfonate microspheres obtained in step (1) were dispersed in 50,000 parts by weight of anhydrous ethanol, and 200 parts by weight of methyl methacrylate and 5 parts by weight of azobisisobutyronitrile were added and mixed thoroughly. The mixture was stirred continuously and heated to 90°C for 3 hours. The reaction was carried out under nitrogen protection. Then, 20 parts by weight of 2-(trifluoromethyl)acrylic acid, 100 parts by weight of divinylbenzene and 2 parts by weight of azobisisobutyronitrile were added. The mixture was stirred continuously and heated to 90°C for 3 hours. The reaction was carried out under nitrogen protection. Finally, the mixture was filtered and dried to obtain multilayer coated liquid metal core-shell particles.

[0071] (3) Preparation of self-powered microspheres for promoting wound healing: (3) The multi-layered liquid metal core-shell particles obtained in step (2) were dispersed in deionized water. The content of liquid metal core-shell particles in deionized water was 0.01 g / mL. Sodium hydroxide was added to adjust the pH of the dispersion to 10-11. The dispersion was stirred and etched at room temperature for 12 h. Then, the dispersion was placed in a dialysis bag and dialyzed for 4 days. Finally, the dispersion was dried to obtain self-powered microspheres for promoting wound healing.

[0072] Application of self-powered wound healing microspheres: 0.5g of self-powered wound healing microspheres were evenly sprinkled on a 4mm diameter wound on the back of a mouse. The wound recovery is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe hair follicle regeneration. The hair follicle activation is shown in Table 2.

[0073] Example 7

[0074] (1) Preparation of liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres: 100 parts by weight of liquid metal at 6℃ was ultrasonically pulverized in an aqueous solution of 50 parts by weight of sodium vinylbenzenesulfonate, and liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres were obtained by filtration and drying.

[0075] (2) Preparation of multilayer coated liquid metal core-shell particles: The liquid metal / sodium polyvinylbenzenesulfonate microspheres obtained in step (1) were dispersed in 50,000 parts by weight of anhydrous ethanol, and 200 parts by weight of methyl acrylate and 5 parts by weight of azobisisobutyronitrile were added and mixed thoroughly. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Then, 50 parts by weight of 2-(trifluoromethyl)acrylic acid, 500 parts by weight of divinylbenzene and 5 parts by weight of azobisisobutyronitrile were added. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Finally, the mixture was filtered and dried to obtain multilayer coated liquid metal core-shell particles.

[0076] (3) Preparation of self-powered microspheres for promoting wound healing: (3) The multi-layered liquid metal core-shell particles obtained in step (2) were dispersed in deionized water. The content of liquid metal core-shell particles in deionized water was 0.01 g / mL. Sodium hydroxide was added to adjust the pH of the dispersion to 10-11. The dispersion was stirred and etched at room temperature for 12 h. Then, the dispersion was placed in a dialysis bag and dialyzed for 4 days. Finally, the dispersion was dried to obtain self-powered microspheres for promoting wound healing.

[0077] (4) Application of self-powered microspheres for promoting wound healing: Take 0.4g of self-powered microspheres for promoting wound healing, mix them with 0.1g of erythromycin ointment, and apply them evenly to a 4mm diameter wound on the back of a mouse. The wound recovery is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe hair follicle regeneration. The hair follicle activation is shown in Table 2.

[0078] Example 8

[0079] (1) Preparation of liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres: 100 parts by weight of liquid metal at 6℃ was ultrasonically pulverized in an aqueous solution of 50 parts by weight of sodium vinylbenzenesulfonate, and liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres were obtained by filtration and drying.

[0080] (2) Preparation of multilayer coated liquid metal core-shell particles: The liquid metal / sodium polyvinylbenzenesulfonate microspheres obtained in step (1) were dispersed in 50,000 parts by weight of anhydrous ethanol, and 200 parts by weight of methyl acrylate and 5 parts by weight of azobisisobutyronitrile were added and mixed thoroughly. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Then, 50 parts by weight of 2-(trifluoromethyl)acrylic acid, 500 parts by weight of divinylbenzene and 5 parts by weight of azobisisobutyronitrile were added. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Finally, the mixture was filtered and dried to obtain multilayer coated liquid metal core-shell particles.

[0081] (3) Preparation of self-powered microspheres for promoting wound healing: (3) The multi-layered liquid metal core-shell particles obtained in step (2) were dispersed in deionized water. The content of liquid metal core-shell particles in deionized water was 0.01 g / mL. Sodium hydroxide was added to adjust the pH of the dispersion to 10-11. The dispersion was stirred and etched at room temperature for 12 h. Then, the dispersion was placed in a dialysis bag and dialyzed for 4 days. Finally, the dispersion was dried to obtain self-powered microspheres for promoting wound healing.

[0082] (4) Application of self-powered microspheres for promoting wound healing: Take 0.4g of self-powered microspheres for promoting wound healing, mix them with 0.1g of Jingwanhong ointment, and apply them evenly to a 4mm diameter wound on the back of a mouse. The wound recovery is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe hair follicle regeneration. The hair follicle activation is shown in Table 2.

[0083] Example 9

[0084] (1) Preparation of liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres: 100 parts by weight of liquid metal at 6℃ was ultrasonically pulverized in an aqueous solution of 50 parts by weight of sodium vinylbenzenesulfonate, and liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres were obtained by filtration and drying.

[0085] (2) Preparation of multilayer coated liquid metal core-shell particles: The liquid metal / sodium polyvinylbenzenesulfonate microspheres obtained in step (1) were dispersed in 50,000 parts by weight of anhydrous ethanol, and 200 parts by weight of methyl acrylate and 5 parts by weight of azobisisobutyronitrile were added and mixed thoroughly. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Then, 50 parts by weight of 2-(trifluoromethyl)acrylic acid, 500 parts by weight of divinylbenzene and 5 parts by weight of azobisisobutyronitrile were added. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Finally, the mixture was filtered and dried to obtain multilayer coated liquid metal core-shell particles.

[0086] (3) Preparation of self-powered microspheres for promoting wound healing: (3) The multi-layered liquid metal core-shell particles obtained in step (2) were dispersed in deionized water. The content of liquid metal core-shell particles in deionized water was 0.01 g / mL. Sodium hydroxide was added to adjust the pH of the dispersion to 10-11. The dispersion was stirred and etched at room temperature for 12 h. Then, the dispersion was placed in a dialysis bag and dialyzed for 4 days. Finally, the dispersion was dried to obtain self-powered microspheres for promoting wound healing.

[0087] (4) Application of self-powered microspheres for promoting wound healing: Take 0.4g of self-powered microspheres for promoting wound healing, mix them with 0.1g of mupirocin ointment and apply them evenly to a 4mm diameter wound on the back of a mouse. The wound recovery is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe hair follicle regeneration. The hair follicle activation is shown in Table 2.

[0088] Example 10

[0089] (1) Preparation of liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres: 100 parts by weight of liquid metal at 6℃ was ultrasonically pulverized in an aqueous solution of 50 parts by weight of sodium vinylbenzenesulfonate, and liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres were obtained by filtration and drying.

[0090] (2) Preparation of multilayer coated liquid metal core-shell particles: The liquid metal / sodium polyvinylbenzenesulfonate microspheres obtained in step (1) were dispersed in 50,000 parts by weight of anhydrous ethanol, and 200 parts by weight of methyl acrylate and 5 parts by weight of azobisisobutyronitrile were added and mixed thoroughly. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Then, 50 parts by weight of 2-(trifluoromethyl)acrylic acid, 500 parts by weight of divinylbenzene and 5 parts by weight of azobisisobutyronitrile were added. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Finally, the mixture was filtered and dried to obtain multilayer coated liquid metal core-shell particles.

[0091] (3) Preparation of self-powered microspheres for promoting wound healing: (3) The multi-layered liquid metal core-shell particles obtained in step (2) were dispersed in deionized water. The content of liquid metal core-shell particles in deionized water was 0.01 g / mL. Sodium hydroxide was added to adjust the pH of the dispersion to 10-11. The dispersion was stirred and etched at room temperature for 12 h. Then, the dispersion was placed in a dialysis bag and dialyzed for 4 days. Finally, the dispersion was dried to obtain self-powered microspheres for promoting wound healing.

[0092] (4) Application of self-powered microspheres for promoting wound healing: Take 0.4g of self-powered microspheres for promoting wound healing, mix them with 0.1g of recombinant human epidermal growth factor gel, and apply them evenly to a 4mm diameter wound on the back of a mouse. The wound recovery is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe hair follicle regeneration. The hair follicle activation is shown in Table 2.

[0093] Example 11

[0094] (1) Preparation of liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres: 100 parts by weight of liquid metal at 6℃ was ultrasonically pulverized in an aqueous solution of 50 parts by weight of sodium vinylbenzenesulfonate, and liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres were obtained by filtration and drying.

[0095] (2) Preparation of multilayer coated liquid metal core-shell particles: The liquid metal / sodium polyvinylbenzenesulfonate microspheres obtained in step (1) were dispersed in 50,000 parts by weight of anhydrous ethanol, and 200 parts by weight of methyl acrylate and 5 parts by weight of azobisisobutyronitrile were added and mixed thoroughly. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Then, 50 parts by weight of 2-(trifluoromethyl)acrylic acid, 500 parts by weight of divinylbenzene and 5 parts by weight of azobisisobutyronitrile were added. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Finally, the mixture was filtered and dried to obtain multilayer coated liquid metal core-shell particles.

[0096] (3) Preparation of self-powered microspheres for promoting wound healing: (3) The multi-layered liquid metal core-shell particles obtained in step (2) were dispersed in deionized water. The content of liquid metal core-shell particles in deionized water was 0.01 g / mL. Sodium hydroxide was added to adjust the pH of the dispersion to 10-11. The dispersion was stirred and etched at room temperature for 12 h. Then, the dispersion was placed in a dialysis bag and dialyzed for 4 days. Finally, the dispersion was dried to obtain self-powered microspheres for promoting wound healing.

[0097] (4) Application of self-powered microspheres for promoting wound healing: Take 0.4g of self-powered microspheres for promoting wound healing, mix them with 0.1g of povidone-iodine, and apply them evenly to a 4mm diameter wound on the back of a mouse. The wound recovery is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe hair follicle regeneration. The hair follicle activation is shown in Table 2.

[0098] Example 12

[0099] (1) Preparation of liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres: 100 parts by weight of liquid metal at 6℃ was ultrasonically pulverized in an aqueous solution of 50 parts by weight of sodium vinylbenzenesulfonate, and liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres were obtained by filtration and drying.

[0100] (2) Preparation of multilayer coated liquid metal core-shell particles: The liquid metal / sodium polyvinylbenzenesulfonate microspheres obtained in step (1) were dispersed in 50,000 parts by weight of anhydrous ethanol, and 200 parts by weight of methyl acrylate and 5 parts by weight of azobisisobutyronitrile were added and mixed thoroughly. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Then, 50 parts by weight of 2-(trifluoromethyl)acrylic acid, 500 parts by weight of divinylbenzene and 5 parts by weight of azobisisobutyronitrile were added. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Finally, the mixture was filtered and dried to obtain multilayer coated liquid metal core-shell particles.

[0101] (3) Preparation of self-powered microspheres for promoting wound healing: (3) The multi-layered liquid metal core-shell particles obtained in step (2) were dispersed in deionized water. The content of liquid metal core-shell particles in deionized water was 0.01 g / mL. Sodium hydroxide was added to adjust the pH of the dispersion to 10-11. The dispersion was stirred and etched at room temperature for 12 h. Then, the dispersion was placed in a dialysis bag and dialyzed for 4 days. Finally, the dispersion was dried to obtain self-powered microspheres for promoting wound healing.

[0102] (4) Application of self-powered microspheres for promoting wound healing: Take 0.4g of self-powered microspheres for promoting wound healing, mix them with 0.1g of Kangfuxin solution and apply them evenly to a wound with a diameter of 4mm on the back of a mouse. The wound recovery is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe hair follicle regeneration. The hair follicle activation is shown in Table 2.

[0103] Example 13

[0104] (1) Preparation of liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres: 100 parts by weight of liquid metal at 6℃ was ultrasonically pulverized in an aqueous solution of 50 parts by weight of sodium vinylbenzenesulfonate, and liquid metal / sodium polyvinylbenzenesulfonate core-shell microspheres were obtained by filtration and drying.

[0105] (2) Preparation of multilayer coated liquid metal core-shell particles: The liquid metal / sodium polyvinylbenzenesulfonate microspheres obtained in step (1) were dispersed in 50,000 parts by weight of anhydrous ethanol, and 200 parts by weight of methyl acrylate and 5 parts by weight of azobisisobutyronitrile were added and mixed thoroughly. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Then, 50 parts by weight of 2-(trifluoromethyl)acrylic acid, 500 parts by weight of divinylbenzene and 5 parts by weight of azobisisobutyronitrile were added. The mixture was stirred continuously and heated to 70°C for 6 hours. The reaction was carried out under nitrogen protection. Finally, the mixture was filtered and dried to obtain multilayer coated liquid metal core-shell particles.

[0106] (3) Preparation of self-powered microspheres for promoting wound healing: (3) The multi-layered liquid metal core-shell particles obtained in step (2) were dispersed in deionized water. The content of liquid metal core-shell particles in deionized water was 0.01 g / mL. Sodium hydroxide was added to adjust the pH of the dispersion to 10-11. The dispersion was stirred and etched at room temperature for 12 h. Then, the dispersion was placed in a dialysis bag and dialyzed for 4 days. Finally, the dispersion was dried to obtain self-powered microspheres for promoting wound healing.

[0107] (4) Application of self-powered microspheres for promoting wound healing: Take 0.4g of self-powered microspheres for promoting wound healing, mix them with 0.1g of Befuji spray, and apply them evenly to a 4mm diameter wound on the back of a mouse. The wound recovery is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe hair follicle regeneration. The hair follicle activation is shown in Table 2.

[0108] Comparative Example 1

[0109] Keeping all other aspects unchanged in Example 1 (1) and (2), after preparing and drying the multi-layered liquid metal core-shell particles, no etching was performed. 0.5g was taken and directly and evenly sprinkled on the wound with a diameter of 4mm on the back of the mouse. The wound recovery status is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe the hair follicle regeneration. The hair follicle activation status is shown in Table 2.

[0110] Comparative Example 2

[0111] Keeping all other aspects unchanged from Example 1, without adding liquid metal, sodium vinylbenzenesulfonate, methyl acrylate, and azobisisobutyronitrile were prepared into poly(sodium vinylbenzenesulfonate-methyl acrylate) in anhydrous ethanol. Then, 2-(trifluoromethyl)acrylic acid, divinylbenzene, and azobisisobutyronitrile were added to react and prepare multilayer polymer microspheres. The polymer shell of the self-powered wound-healing microspheres was then etched. 0.5g of the mixture was directly and evenly applied to a 4mm diameter wound on the back of mice. The wound healing status is shown in Table 1. Nine days later, skin tissue was taken for H&E staining to observe hair follicle regeneration. The hair follicle activation status is shown in Table 2.

[0112] Comparative Example 3

[0113] Keeping everything else unchanged in Example 1, in step (2), benzoyl acrylate and azobisisobutyronitrile were not added for the two-layer coating. Instead, 2-(trifluoromethyl)acrylic acid, divinylbenzene, and azobisisobutyronitrile were directly added for coating, and the subsequent etching steps were completed to obtain bilayer liquid metal microspheres. 0.5g was taken and evenly sprinkled on a 4mm diameter wound on the back of a mouse. The wound recovery is shown in Table 1. After 9 days, skin tissue was taken for H&E staining to observe hair follicle regeneration. The hair follicle activation is shown in Table 2.

[0114] Comparative Example 4

[0115] Microspheres that do not require self-powered wound healing were used. Only 0.5g of erythromycin ointment was applied to the wounds of mice, and the wound recovery was shown in Table 1. Nine days later, skin tissue was taken for H&E staining to observe hair follicle regeneration; the activation status is shown in Table 2.

[0116] Comparative Example 5

[0117] Microspheres that do not require self-powered wound healing were used. Only 0.5g of Jingwanhong ointment was applied to the wounds of mice, and the wound recovery was shown in Table 1. Nine days later, skin tissue was taken for H&E staining to observe hair follicle regeneration; the activation status is shown in Table 2.

[0118] Comparative Example 6

[0119] Microspheres that do not require self-powered wound healing were used. Only 0.5g of mobirocin ointment was applied to the wounds of mice, and the wound recovery was shown in Table 1. Nine days later, skin tissue was taken for H&E staining to observe hair follicle regeneration; the activation status is shown in Table 2.

[0120] Comparative Example 7

[0121] Microspheres that do not require self-powered wound healing were used. Only 0.5g of recombinant human epidermal growth factor gel was applied to the wounds of mice. The wound recovery is shown in Table 1. Nine days later, skin tissue was taken for H&E staining to observe hair follicle regeneration. The activation status is shown in Table 2.

[0122] Comparative Example 8

[0123] Microspheres that do not require self-powered wound healing were applied to mouse wounds using only 0.5g of povidone-iodine. The wound recovery is shown in Table 1. Skin tissue was taken 9 days later for H&E staining to observe hair follicle regeneration. The activation status is shown in Table 2.

[0124] Comparative Example 9

[0125] Microspheres that do not require self-powered wound healing were used. Only 0.5g of Kangfuxin solution was applied to the wounds of mice, and the wound recovery was shown in Table 1. Nine days later, skin tissue was taken for H&E staining to observe hair follicle regeneration; the activation status is shown in Table 2.

[0126] Comparative Example 10

[0127] Microspheres that do not require self-powered wound healing were applied to mouse wounds using only 0.5g of Befuji spray. The wound recovery is shown in Table 1. Skin tissue was taken 9 days later for H&E staining to observe hair follicle regeneration. The activation status is shown in Table 2.

[0128] Table 1 Wound healing rate

[0129]

[0130] Table 2 Hair follicle regeneration status (9 days)

[0131]

[0132] Comparing Tables 1 and 2, Comparative Example 1, using multi-layered coated liquid metal core-shell particles, showed slower wound healing and no hair follicle regeneration after 9 days. This demonstrates that etching creates extra space within the microparticles, increasing the vibratory space of the liquid metal, which is beneficial for improving self-generating power and promoting better wound repair and hair follicle regeneration. Comparative Example 2, using microspheres with a polymer shell for self-powered wound healing, lacked triboelectric power generation due to the absence of liquid metal, resulting in slower wound healing and no new hair follicle formation. This demonstrates that electrical stimulation promotes wound repair and hair follicle activation, contributing to scarless healing. Comparative Example 3, using microspheres without paraben coating, showed that the inner layer was partially cross-linked due to the infiltration of the outermost cross-linking agent, preventing the creation of a large cavity and limiting the fluidity of the liquid metal. This resulted in poor self-generating power, slower healing, and no new hair follicle formation. This demonstrates that the second layer of paraben coating helps increase the internal cavity volume of the microspheres, improving self-generating power.

[0133] Comparative Examples 4-10, treated with erythromycin ointment, Jingwanhong ointment, mupirocin ointment, recombinant human epidermal growth factor gel, povidone-iodine, Kangfuxin solution, and Beifuji spray respectively, showed slower wound healing and no hair follicle regeneration after 9 days. In contrast, Examples 7-13, using self-powered wound healing microspheres in synergy with commercially available drugs, showed significantly better wound healing than Comparative Examples 4-10. This demonstrates that the self-powered wound healing microspheres can effectively improve wound healing, achieving good wound repair and activating hair follicles to inhibit scar hyperplasia. Furthermore, compared to Examples 1-6, Examples 7-13 showed better results using self-powered wound healing microspheres in synergy with commercially available drugs than using only the microspheres themselves, with improved wound healing rates on days 3 and 6. This demonstrates that the self-powered wound healing microspheres and commercially available drugs have a synergistic effect in promoting tissue healing.

Claims

1. A method for preparing self-powered wound healing-promoting microspheres, characterized by, The preparation method comprises the following steps: (1) ultrasonic grinding liquid metal in the aqueous solution of sodium 4-vinylbenzenesulfonate, and obtaining liquid metal / polyvinylbenzenesulfonic acid sodium core-shell microspheres by filtration and drying; (2) dispersing the liquid metal / polyvinylbenzenesulfonic acid sodium core-shell microspheres in step (1) in anhydrous ethanol, and adding benzyl acrylate and an initiator to mix uniformly, and stirring to react, wherein the reaction system is carried out under nitrogen protection; then, adding fluorine-containing acrylic monomer, divinylbenzene and an initiator, and continuously stirring, wherein the reaction system is carried out under nitrogen protection; finally, filtering and drying to obtain liquid metal core-shell particles coated with multiple layers; (3) dispersing the liquid metal core-shell particles coated with multiple layers obtained in step (2) in deionized water, adjusting the pH of the dispersion to 10-11, and continuously stirring to etch at room temperature; then, placing the dispersion into a dialysis bag to dialyze; finally, drying to obtain self-powered microspheres for promoting wound healing.

2. The method for preparing self-powered wound-healing microspheres according to claim 1, characterized in that, The various raw materials are calculated based on 100 parts by weight of the liquid metal, 10-100 parts by weight of sodium 4-vinylbenzenesulfonate, 100-400 parts by weight of benzyl acrylate, 20-200 parts by weight of fluorine-containing acrylic monomer, 100-1000 parts by weight of divinylbenzene, 2-15 parts by weight of the initiator added each time, and 20000-100000 parts by weight of anhydrous ethanol.

3. The method for preparing self-powered wound-healing microspheres according to claim 1, characterized in that, The liquid metal in step (1) is one of gallium-indium alloys with a melting point of-19℃-25℃.

4. The method for preparing self-powered wound-healing microspheres according to claim 1, characterized in that, The fluorine-containing acrylic monomer in step (2) is one of 2-(trifluoromethyl) acrylate, trifluoroethyl methacrylate and hexafluorobutyl methacrylate; the initiator in step (2) is one of azobis isobutyronitrile, azobis isoheptyl nitrile and dibenzoyl peroxide; in step (2), the reaction time of the liquid metal / polyvinylbenzenesulfonic acid sodium core-shell microsphere dispersion, benzyl acrylate and the initiator is 3-10h, and the temperature is 50-90℃; after adding the fluorine-containing acrylic monomer, divinylbenzene and the initiator, the reaction time in step (2) is 3-10h, and the temperature is 50-90℃.

5. The method for preparing self-powered wound-healing microspheres according to claim 1, characterized in that, In step (2), the pH of the dispersion is adjusted to 10-11 by sodium hydroxide or potassium hydroxide.

6. The method for preparing self-powered wound-healing microspheres according to claim 1, characterized in that, In step (3), the content of the liquid metal core-shell particles coated with multiple layers in deionized water is 0.01-0.1g / mL, and the etching time is 8-24h; the dialysis time is 3-5 days.

7. The method for preparing self-powered microspheres for promoting wound healing according to claim 1, characterized in that, The particle size of the self-powered microspheres for promoting wound healing is 50-1500nm.

8. The self-powered wound healing promoting microspheres prepared by the method of any one of claims 1 to 7, characterized in that, The liquid metal inside the self-powered microspheres for promoting wound healing has good deformation ability and drivability, and the skin shaking or mechanical movement of the body can drive the liquid metal to oscillate to realize friction power generation with the outer shell; the self-powered microspheres for promoting wound healing have no cytotoxicity, can improve the electrical signal at the wound, promote collagen and hair follicle regeneration, reduce wound inflammation, and help to promote wound healing and reduce scar formation on the wound surface.

9. The self-powered microspheres for promoting wound healing prepared by the preparation method of any one of claims 1-7 are used for preparing medical materials for promoting wound healing.

10. Use according to claim 9, characterized in that, The medical material is self-powered microspheres for promoting wound healing mixed with a topical ointment or liquid medicine to prepare a self-powered healing composite material.

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