Polymer nitrogen compound microbial liquid fertilizer for ligusticum wallichii cultivation and preparation method thereof

Abstract

The invention discloses a polymer nitrogen compound microbial liquid fertilizer for ligusticum wallichii cultivation and a preparation method thereof, and relates to the technical field of fertilizers, the preparation method of the liquid fertilizer comprises the following steps: mixing brown sugar, table vinegar, soybean meal and water as a fermentation substrate; inoculating a compound flora for anaerobic fermentation to obtain a fermented compound microorganism bacterial liquid; ammonia water, urea, citric acid, monopotassium phosphate, magnesium sulfate and ferrous sulfate are added into formaldehyde for a reaction, and a urea formaldehyde polymer liquid compound fertilizer is obtained; adding a protective agent into the fermented compound microorganism bacterial liquid to obtain a compound strain protective liquid; and compounding with a urea formaldehyde polymer liquid compound fertilizer step by step. The liquid fertilizer meets the requirements of ligusticum wallichii in the whole growth period through mutual promotion of the slow release characteristic of urea formaldehyde nitrogen and the metabolic activity of compound microorganisms, is beneficial to yield increase of ligusticum wallichii and accumulation of effective components, can reduce the fertilizer dosage and the fertilization frequency, reduces disturbance of traditional fertilizer application to the soil environment, and improves the yield of ligusticum wallichii. The method has important production and application values.

Description

Technical Field

[0001] This invention relates to the field of fertilizer technology, specifically to a high-molecular nitrogen compound microbial liquid fertilizer for the cultivation of Ligusticum chuanxiong and its preparation method. Background Technology

[0002] Ligusticum chuanxiong is a well-known and widely used traditional Chinese medicine, with its rhizome used medicinally. It is believed to have the effects of promoting blood circulation, regulating qi, dispelling wind, and relieving pain, and is in high demand in the market. In its large-scale cultivation, the growth and development of Ligusticum chuanxiong, as well as the formation of its medicinal quality, have specific requirements for nutrient supply. It not only requires sufficient macronutrients such as nitrogen, phosphorus, and potassium, but is also highly sensitive to micronutrients such as magnesium and iron. Furthermore, as a rhizome-based medicinal herb, its yield and the accumulation of its active ingredients (such as ferulic acid and ligustilide) are closely related to the health of the rhizosphere soil microecological environment.

[0003] Currently, given the unique nutrient requirements of Ligusticum chuanxiong, production still largely relies on traditional compound fertilizers with fixed nutrient ratios and single functions, making precise supply difficult. While these fertilizers can provide some nutrients, long-term application can easily lead to soil compaction and acidification, damaging soil aggregate structure and microbial community diversity, thereby causing a series of problems such as increased soil-borne diseases, reduced fertilizer utilization, and eutrophication of water bodies. More importantly, the unbalanced soil environment directly inhibits the development of Ligusticum chuanxiong roots and the synthesis of secondary metabolites, resulting in a decrease in the content of key medicinal components, seriously affecting the quality and clinical efficacy of the medicinal material.

[0004] Liquid fertilizers have garnered widespread attention due to their uniform nutrient distribution, ease of plant absorption, and suitability for integrated water and fertilizer application, thereby improving labor efficiency. Existing liquid fertilizers are mainly classified into clear liquid and suspension types. While suspension fertilizers can hold higher nutrient concentrations, they suffer from inherent drawbacks such as poor physical stability, susceptibility to sedimentation, and tendency to clog drip irrigation nozzles, limiting their widespread application in precision irrigation systems. Clear liquid fertilizers, on the other hand, exhibit excellent stability and are less prone to sedimentation; however, their core limitation lies in maintaining a clear liquid state, which typically results in lower nutrient content. Furthermore, introducing functional microorganisms into fertilizer systems is an effective way to improve rhizosphere microecology and enhance crop resistance and nutrient utilization efficiency. However, microbial agents are highly susceptible to inactivation due to cell dehydration in high-osmotic-pressure liquid environments. When coexisting with high concentrations of inorganic salt ions, their cell membranes and enzyme systems suffer severe stress, leading to a sharp decline in survival rate and activity. This core technological bottleneck—the incompatibility between microbial activity and high salt and nutrient concentrations—severely restricts the development and application of highly efficient compound microbial liquid fertilizers that integrate chemical nutrition and biological regulation.

[0005] Therefore, there is an urgent need in this field for an innovative liquid fertilizer that can provide comprehensive and balanced slow-release nutrition tailored to the nutrient requirements of Ligusticum chuanxiong, while also carrying highly active beneficial microbial communities to optimize the rhizosphere microecology. Simultaneously, this product must possess high liquid stability and be suitable for modern irrigation systems. Developing such a product is of great significance for improving the yield and quality of Ligusticum chuanxiong, reducing non-point source pollution from chemical fertilizers, and promoting the ecological cultivation of Chinese medicinal herbs. Summary of the Invention

[0006] To address the aforementioned technical problems, the present invention aims to provide a high-molecular nitrogen compound microbial liquid fertilizer for Ligusticum chuanxiong cultivation and its preparation method. This liquid fertilizer, through the slow-release characteristics of urea-formaldehyde nitrogen and the synergistic promotion of the metabolic activities of the compound microorganisms, meets the needs of Ligusticum chuanxiong throughout its entire growth cycle, promoting increased yield and accumulation of effective components. Simultaneously, it reduces fertilizer usage and application frequency, minimizing the disturbance to the soil environment caused by traditional fertilizer application, thus possessing significant production and application value.

[0007] The technical solution of this invention to solve the above-mentioned technical problems is as follows: A method for preparing a high-molecular nitrogen compound microbial liquid fertilizer for Ligusticum chuanxiong cultivation is provided, comprising the following steps: (1) Mix brown sugar, edible vinegar, soybean meal and water to obtain a fermentation substrate; then inoculate a compound microbial community into the fermentation substrate and carry out anaerobic fermentation under closed conditions. After filtration, a fermentation compound microbial liquid is obtained. (2) Add ammonia to formaldehyde and stir to adjust the pH to 7.5-8. After heating, add urea to react. Then add citric acid to adjust the pH to 5.8-6.2. Then add potassium dihydrogen phosphate, magnesium sulfate and ferrous sulfate to react. Cool to room temperature to obtain urea-formaldehyde high molecular weight liquid compound fertilizer. (3) Mix the fermentation compound microbial liquid obtained in step (1), trehalose and glycine betaine to obtain a compound microbial strain protection solution; (4) Mix the compound microbial protection liquid obtained in step (3) with the first portion of the urea-formaldehyde polymer liquid compound fertilizer obtained in step (2) evenly, and then add the second portion of the urea-formaldehyde polymer liquid compound fertilizer obtained in step (2) and mix evenly to obtain the polymer nitrogen compound microbial liquid fertilizer for Ligusticum chuanxiong cultivation.

[0008] Furthermore, in step (1), the mass ratio of brown sugar, edible vinegar, soybean meal and water is 5-30:1-10:1-5:100.

[0009] Furthermore, in step (1), the edible vinegar is at least one of rice vinegar, aged vinegar, white vinegar or balsamic vinegar, and the acetic acid content is not less than 3.5 t.

[0010] Furthermore, in step (1), the complex microbial community includes yeast, lactic acid bacteria and acetic acid bacteria.

[0011] Furthermore, in step (1), anaerobic fermentation is carried out at a temperature of 28-32℃ for 15-20 days.

[0012] Furthermore, in step (1), the number of viable lactic acid bacteria in the fermentation compound microbial liquid is not less than 5.0 × 10⁻⁶. 8 CFU / mL, yeast viable count not less than 1.0 × 10⁻⁶ 8 CFU / mL, viable acetic acid bacteria count not less than 1.0 × 10⁻⁶ 8 CFU / mL.

[0013] Furthermore, the viable count of lactic acid bacteria in the fermentation compound microbial broth was 5.0 × 10⁻⁶. 8 -5×10 9 CFU / mL, yeast viable count 1.0-9.0×10⁻⁶ 8 CFU / mL, viable acetic acid bacteria count 1.0-9.0×10⁻⁶ 8 CFU / mL.

[0014] Furthermore, in step (2), the mass ratio of formaldehyde, urea, potassium dihydrogen phosphate, magnesium sulfate and ferrous sulfate is 10-20:20-60:5-15:0.5-5:0.5-5.

[0015] Furthermore, in step (2), the temperature is increased to 85-90°C.

[0016] Furthermore, in step (2), after adding urea, the reaction time is 10-90 min; after adding potassium dihydrogen phosphate, magnesium sulfate and ferrous sulfate, the reaction time is 30-60 min.

[0017] Furthermore, in step (3), the mass ratio of the fermentation compound microbial liquid, trehalose and glycine betaine is 100:1-10:1-10.

[0018] Furthermore, in step (4), the mass ratio of the compound microbial inoculum protection solution, the first portion of urea-formaldehyde polymer liquid compound fertilizer, and the second portion of urea-formaldehyde polymer liquid compound fertilizer is 100:100:200-1000.

[0019] Furthermore, step (4) is carried out at a stirring speed of less than 100 rpm and a temperature of 25-30℃.

[0020] The beneficial effects of adopting further technical solutions are: in the compounding process, by controlling the temperature below 35℃ and adding trehalose and glycine betaine as protective agents, combined with a stepwise compounding process, the survival rate of compound microorganisms can be ensured to be no less than 90%.

[0021] The present invention also provides a high molecular weight nitrogen compound microbial liquid fertilizer for Ligusticum chuanxiong cultivation prepared by the above-mentioned method.

[0022] This invention also provides the application of the above-mentioned high-molecular nitrogen compound microbial liquid fertilizer for Ligusticum chuanxiong cultivation in the cultivation of Ligusticum chuanxiong.

[0023] The present invention also provides a method for cultivating Ligusticum striatum, wherein the above-mentioned high molecular weight nitrogen compound microbial liquid fertilizer for Ligusticum striatum cultivation is applied when cultivating Ligusticum striatum. Specifically, apply 5-7 kg / mu when the plant has two leaves and one bud, 8-12 kg / mu after an interval of 6-8 days, 13-17 kg / mu during the stem and leaf development stage, 18-20 kg / mu during the second stem and leaf development stage, and 13-17 kg / mu one month before the root and stem enlargement stage; dilute 80-120 times when applying; apply by root irrigation.

[0024] The present invention has the following beneficial effects: 1. This invention uses natural raw materials such as brown sugar, edible vinegar, and soybean meal powder for compound fermentation. The resulting microbial agent is rich in lactic acid bacteria, acetic acid bacteria, and yeast. The multi-microbial system has a significant synergistic effect: the organic acids produced by the metabolism of lactic acid bacteria and acetic acid bacteria can reduce the pH value of plant rhizosphere, effectively inhibit the reproduction of soil-borne pathogens, and activate nutrients such as phosphorus and potassium fixed in the soil; yeast secretes growth-promoting substances such as vitamins and amino acids, which together promote the development of Ligusticum chuanxiong roots and nutrient absorption. This fermentation process does not require purification inoculation or strict aseptic conditions, and has comprehensive advantages such as low cost, easy large-scale production, and strong adaptability of the microbial community ecology.

[0025] 2. In the synthesis of urea-formaldehyde polymer nitrogen fertilizer, this invention introduces nutrients such as potassium dihydrogen phosphate, magnesium sulfate, and ferrous sulfate in situ. This allows phosphorus, potassium, magnesium, and iron to be effectively integrated, encapsulated, and complexed during the polymer network formation stage, forming an organic-inorganic composite system. This reduces the system's salt index and fundamentally avoids the salting-out, crystallization, and precipitation problems that are easily caused by conventional physical mixing, ensuring the high-solid stability of the product. At the same time, the integrated nutrients have better slow-release performance, which works synergistically with the slow-release characteristics of urea-formaldehyde nitrogen to jointly improve the long-term supply capacity and utilization rate of nutrients.

[0026] 3. This invention combines fermented compound microbial broth with low-salt-index urea-formaldehyde polymer liquid compound fertilizer, and adds trehalose as a microbial protectant to construct a suitable liquid environment for microbial survival. The encapsulation and complexation of inorganic salts by urea-formaldehyde polymers significantly reduces the ionic strength of the system, while trehalose can form a protective layer on the cell surface, jointly alleviating the stress of high osmotic pressure on microorganisms, effectively improving the survival rate and colonization ability of microorganisms in liquid fertilizer, and achieving long-term stable coexistence of functional microorganisms and high-concentration nutrients.

[0027] 4. The slow-release properties of urea-formaldehyde nitrogen and the metabolic activities of the compound microorganisms mutually promote each other, dynamically releasing nutrients according to the growth stages of Ligusticum chuanxiong to meet its needs throughout its entire growth period, significantly reducing nutrient volatilization and leaching losses. Based on the fertilizer's continuous nutrient supply capacity, the number of fertilizations and the total amount of fertilizer applied throughout the entire growth period of Ligusticum chuanxiong are reduced. While simplifying field management and saving labor, this significantly reduces environmental pollution caused by excessive fertilization, achieving a balance between economic and ecological benefits. Attached Figure Description

[0028] Figure 1 A comparison of the aboveground dry weight of Ligusticum chuanxiong at different growth stages; Figure 2 A comparison chart of the dry weight of Ligusticum chuanxiong tubers at different growth stages; Figure 3 A comparison chart of the dry weight of the fibrous roots of Ligusticum chuanxiong at different growth stages; Figure 4 A comparison chart of the dry weight of a single plant of Ligusticum chuanxiong at different growth stages; Figure 5 Comparison of photosynthetic pigment content in Ligusticum chuanxiong under different fertilization treatments; Figure 6 A comparison chart of soluble protein and soluble sugar content in Ligusticum chuanxiong under different fertilization treatments; Figure 7 Comparison of amylase and Rubisco enzyme content in Ligusticum chuanxiong under different fertilization treatments; Figure 8 A comparison chart of the yield of Ligusticum chuanxiong under different fertilization treatments; Figure 9 A comparison chart of brassinolide content in Ligusticum chuanxiong under different fertilization treatments; Figure 10 A comparison chart of the effective component content of Ligusticum chuanxiong under different fertilization treatments. Detailed Implementation

[0029] The principles and features of this invention are described below. The examples given are for illustrative purposes only and are not intended to limit the scope of the invention. Unless otherwise specified in the examples, conventional conditions or conditions recommended by the manufacturer should be followed. Reagents or instruments whose manufacturers are not specified are all commercially available products.

[0030] Example 1 A high-molecular nitrogen compound microbial liquid fertilizer for the cultivation of Ligusticum chuanxiong, the preparation method of which includes the following steps: (1) Brown sugar, edible vinegar, soybean meal and water were mixed in a mass ratio of 17:5:3:100 to obtain a fermentation substrate; then a compound microbial community was inoculated into the fermentation substrate, and anaerobic fermentation was carried out at 30℃ under sealed conditions for 18 days. After filtration, a fermentation compound microbial liquid was obtained; the compound microbial community consisted of yeast, lactic acid bacteria and acetic acid bacteria; the viable number of lactic acid bacteria in the fermentation compound microbial liquid was 5.0×10⁻⁶. 8 CFU / mL, yeast viable count 5.0 × 10⁻⁶ 8 CFU / mL, viable acetic acid bacteria count was 5.0 × 10⁻⁶. 8 CFU / mL.

[0031] (2) Add ammonia to formaldehyde and stir to adjust the pH to 7.8. Heat to 88°C and add urea to react for 60 min. Then add citric acid to adjust the pH to 6. Add potassium dihydrogen phosphate, magnesium sulfate and ferrous sulfate and react for 40 min. Cool to room temperature to obtain urea-formaldehyde high molecular weight liquid compound fertilizer. The mass ratio of formaldehyde, urea, potassium dihydrogen phosphate, magnesium sulfate and ferrous sulfate is 15:40:10:3:2. (3) Mix the fermentation compound microbial culture obtained in step (1), trehalose and glycine betaine to obtain a compound microbial culture protection solution; the mass ratio of the fermentation compound microbial culture, trehalose and glycine betaine is 100:5:5; (4) Under the conditions of stirring speed of 50 rpm and temperature of 28℃, the compound microbial inoculum protection liquid obtained in step (3) is stirred and mixed evenly with the first part of the urea-formaldehyde polymer liquid compound fertilizer obtained in step (2), and then the second part of the urea-formaldehyde polymer liquid compound fertilizer obtained in step (2) is added and mixed evenly to obtain the polymer nitrogen compound microbial liquid fertilizer for Ligusticum chuanxiong cultivation; the mass ratio of compound microbial inoculum protection liquid, the first part of the urea-formaldehyde polymer liquid compound fertilizer and the second part of the urea-formaldehyde polymer liquid compound fertilizer is 100:100:800.

[0032] Example 2 A high-molecular nitrogen compound microbial liquid fertilizer for the cultivation of Ligusticum chuanxiong, the preparation method of which includes the following steps: (1) Brown sugar, edible vinegar, soybean meal and water were mixed in a mass ratio of 5:1:1:100 to obtain a fermentation substrate; then a compound microbial community was inoculated into the fermentation substrate, and anaerobic fermentation was carried out at 28℃ under sealed conditions for 15 days. After filtration, a fermentation compound microbial liquid was obtained; the compound microbial community consisted of yeast, lactic acid bacteria and acetic acid bacteria; the viable number of lactic acid bacteria in the fermentation compound microbial liquid was 5.0×10⁻⁶. 8 CFU / mL, yeast viable count 1.0 × 10⁻⁶ 8 CFU / mL, viable acetic acid bacteria count 1.0 × 10⁻⁶ 8 CFU / mL.

[0033] (2) Add ammonia to formaldehyde and stir to adjust the pH to 7.5. Heat to 85°C and add urea to react for 10 min. Then add citric acid to adjust the pH to 5.8. Add potassium dihydrogen phosphate, magnesium sulfate and ferrous sulfate and react for 30 min. Cool to room temperature to obtain urea-formaldehyde polymer liquid compound fertilizer. The mass ratio of formaldehyde, urea, potassium dihydrogen phosphate, magnesium sulfate and ferrous sulfate is 10:20:5:0.5:0.5. (3) Mix the fermentation compound microbial culture obtained in step (1), trehalose and glycine betaine to obtain a compound microbial culture protection solution; the mass ratio of fermentation compound microbial culture, trehalose and glycine betaine is 100:1:1; (4) Under the conditions of stirring speed of 50 rpm and temperature of 28℃, the compound microbial inoculum protection liquid obtained in step (3) is stirred and mixed evenly with the first part of the urea-formaldehyde polymer liquid compound fertilizer obtained in step (2), and then the second part of the urea-formaldehyde polymer liquid compound fertilizer obtained in step (2) is added and mixed evenly to obtain the polymer nitrogen compound microbial liquid fertilizer for Ligusticum chuanxiong cultivation; the mass ratio of compound microbial inoculum protection liquid, the first part of the urea-formaldehyde polymer liquid compound fertilizer and the second part of the urea-formaldehyde polymer liquid compound fertilizer is 100:100:200.

[0034] Example 3 A high-molecular nitrogen compound microbial liquid fertilizer for the cultivation of Ligusticum chuanxiong, the preparation method of which includes the following steps: (1) Brown sugar, edible vinegar, soybean meal and water were mixed in a mass ratio of 30:10:5:100 to obtain a fermentation substrate; then a compound microbial community was inoculated into the fermentation substrate, and anaerobic fermentation was carried out at 32℃ under sealed conditions for 20 days. After filtration, a fermentation compound microbial liquid was obtained; the compound microbial community consisted of yeast, lactic acid bacteria and acetic acid bacteria; the number of viable lactic acid bacteria in the fermentation compound microbial liquid was 6.0×10⁻⁶. 8 CFU / mL, yeast viable count 1.0 × 10⁻⁶ 8 CFU / mL, viable acetic acid bacteria count 1.0 × 10⁻⁶ 8 CFU / mL.

[0035] (2) Add ammonia to formaldehyde and stir to adjust the pH to 8. Heat to 90°C and add urea to react for 90 min. Then add citric acid to adjust the pH to 6.2. Add potassium dihydrogen phosphate, magnesium sulfate and ferrous sulfate and react for 60 min. Cool to room temperature to obtain urea-formaldehyde high molecular weight liquid compound fertilizer. The mass ratio of formaldehyde, urea, potassium dihydrogen phosphate, magnesium sulfate and ferrous sulfate is 20:60:15:5:5. (3) Mix the fermentation compound microbial culture obtained in step (1), trehalose and glycine betaine to obtain a compound microbial culture protection solution; the mass ratio of the fermentation compound microbial culture, trehalose and glycine betaine is 100:10:10. (4) Under the conditions of stirring speed of 50 rpm and temperature of 28℃, the compound microbial inoculum protection liquid obtained in step (3) is stirred and mixed evenly with the first part of the urea-formaldehyde polymer liquid compound fertilizer obtained in step (2), and then the second part of the urea-formaldehyde polymer liquid compound fertilizer obtained in step (2) is added and mixed evenly to obtain the polymer nitrogen compound microbial liquid fertilizer for Ligusticum chuanxiong cultivation; the mass ratio of compound microbial inoculum protection liquid, the first part of the urea-formaldehyde polymer liquid compound fertilizer and the second part of the urea-formaldehyde polymer liquid compound fertilizer is 100:100:1000.

[0036] Comparative Example 1 A urea-formaldehyde polymer liquid compound fertilizer is prepared in a way that differs from that in Example 1 in that the compound microbial strain protection liquid in step (4) is replaced with water.

[0037] Experimental Example 1: Cultivation Experiment of Ligusticum chuanxiong In mid-to-late August, Sichuan lovage was planted in the experimental field in Dujiangyan, and the experimental field was divided into three groups, each with 2 mu (approximately 0.33 hectares).

[0038] The first group adopted the traditional fertilizer application scheme, and the specific fertilizer application details are shown in Table 1.

[0039] Table 1 Traditional Fertilizer Application Scheme The second and third groups used fertilizers prepared in Example 1 and Comparative Example 1 for root irrigation. 6 kg / mu was applied when the plant had two leaves and one bud, 10 kg / mu was applied 7 days later, 15 kg / mu was applied during the stem and leaf development period (end of October), 19 kg / mu was applied during the second stem and leaf development period (March of the following year), and 15 kg / mu was applied one month before the root and stem enlargement period (April of the following year); the fertilizers were diluted 100 times before application.

[0040] (1) Plant samples were randomly selected at different times, and the aboveground dry weight, tuber dry weight, fibrous root dry weight, and single plant dry weight of Ligusticum chuanxiong were measured. The results are shown in Table 2 and Figures 1-4 As shown.

[0041] Table 2 Comparison of morphological indicators of Ligusticum chuanxiong plants at different stages The results showed that in the experimental group using the liquid fertilizer of Example 1, the dry weight of the rhizome and the dry weight of the fibrous roots of Ligusticum chuanxiong were basically the same, and both were significantly better than those of Comparative Example 1. For the aboveground dry weight and the dry weight of a single plant, the experimental group using the liquid fertilizer of Example 1 achieved the best results. Furthermore, the application frequency and fertilizer content of the liquid fertilizer in Example 1 were lower than those of traditional fertilization schemes. This indicates that the liquid fertilizer of the present invention, through the slow-release characteristics of urea-formaldehyde nitrogen, provides nutrients sustainably, and, combined with the microbial community, significantly improves the utilization rate of nutrients by Ligusticum chuanxiong.

[0042] (2) In mid-to-late October, leaves from the middle part of the plant were collected to determine the content of photosynthetic pigments, soluble protein, soluble sugar, amylase and Rubisco enzyme activities.

[0043] a. Differences in the content of photosynthetic pigments (chlorophyll and carotenoids) in Ligusticum chuanxiong under different fertilization treatments, such as Figure 5 As shown.

[0044] Depend on Figure 5 It can be seen that the photosynthetic pigment content of Ligusticum chuanxiong increased under different fertilization treatments. Regarding chlorophyll, the treatment groups in Comparative Example 1 and Example 1 showed significantly higher levels than those treated with traditional fertilizers. p <0.001); meanwhile, for carotenoids, the differences among the three groups were all significant ( p <0.05), with the highest photosynthetic pigment content observed in the treatment group treated with the liquid fertilizer of Example 1. This demonstrates that the liquid fertilizer of the present invention has the most significant positive promoting effect on photosynthetic pigment content, which is more conducive to improving the photosynthetic efficiency of plants and thus promoting crop growth.

[0045] b. Differences in the content of soluble protein and soluble sugar in Ligusticum chuanxiong under different fertilization treatments, such as Figure 6 As shown.

[0046] Depend on Figure 6 It can be seen that the differences in soluble protein content among the three treatment groups are extremely significant. p <0.001), the soluble sugar content of the conventional fertilizer and Example 1 was significantly higher than that of Comparative Example 1 ( p <0.001). The results show that the combination of microbial flora and high molecular weight nitrogen added in this invention has a synergistic effect and can positively regulate the content of soluble protein and soluble sugar in Ligusticum chuanxiong.

[0047] c. Differences in amylase and Rubisco enzyme content in Ligusticum chuanxiong under different fertilization treatments, such as Figure 7 As shown.

[0048] Depend on Figure 7 It can be seen that in *Ligusticum chuanxiong* treated with the liquid fertilizers of Example 1 and Comparative Example 1, the levels of amylase and Rubiscoase were significantly higher than those of traditional fertilizers. The results indicate that the liquid fertilizer of this invention promotes photosynthesis in *Ligusticum chuanxiong*, which is beneficial to plant growth.

[0049] (3) Ligusticum chuanxiong was harvested in late May, and the yield (dry weight) was determined. The contents of brassinolide, ferulic acid, and ligustilide in different experimental groups of Ligusticum chuanxiong were detected by high performance liquid chromatography (HPLC). The results are shown in Table 3 and Figures 8-10 As shown.

[0050] Table 3. Yield, growth regulators and active ingredient content of Ligusticum chuanxiong The results showed that the yield performance of the conventional fertilizer was comparable to that of Example 1. p >0.05), and all were significantly higher than Comparative Example 1 ( p <0.01); The content of brassinolide, a growth regulator, in Ligusticum chuanxiong in Example 1 was significantly lower than that in traditional fertilizer and Comparative Example 1, while the contents of the active ingredients ferulic acid and ligustilide were significantly higher than those in traditional fertilizer and Comparative Example 1. p The content of ferulic acid in Ligusticum chuanxiong is less than 0.001%, and it is higher than the pharmacopoeia standard (the pharmacopoeia stipulates that the content of ferulic acid in Ligusticum chuanxiong shall not be less than 0.10%, and the content of ligustilide shall not be less than 0.8%). This indicates that the liquid fertilizer provided by this invention can reduce the content of brassinolide, regulate the vegetative growth of the above-ground parts, and promote the transfer of photosynthetic products to the rhizomes. Its effect is that while reducing the amount of fertilizer applied and optimizing the number of fertilizations, the content of effective components in the produced Ligusticum chuanxiong is significantly higher than that of traditional fertilization schemes, and the yield is comparable, thus balancing economic output and medicinal quality.

[0051] (4) In addition, soil microbial diversity analysis showed that the number of beneficial bacteria (such as actinomycetes) in the soil of the treatment group of Example 1 was significantly higher than that of the control group 1, the fungal / bacterial ratio was lower, and the soil micro-ecological environment was healthier. The fungal / bacterial ratio usually means stronger resistance to disturbance. The mycelial network of fungi helps to form soil aggregates, enhances soil structural stability, water retention and nutrient retention capacity, and makes the ecosystem more stable. It can be seen that the introduction of the complex microbial community of lactic acid bacteria, acetic acid bacteria and yeast in the liquid fertilizer of this invention is beneficial to reducing the disturbance of fertilizer application to the soil environment.

[0052] In summary, the high-molecular-weight nitrogen-based microbial liquid fertilizer for Ligusticum chuanxiong cultivation of this invention, through the slow-release characteristics of urea-formaldehyde nitrogen and the synergistic effect of the metabolic activities of the compound microorganisms, can dynamically release nutrients according to the growth stages of Ligusticum chuanxiong, meeting its needs throughout its entire growth period. Based on the fertilizer's continuous nutrient supply capacity, the frequency and total amount of fertilization during the entire growth period of Ligusticum chuanxiong are reduced. Simultaneously, the application of this liquid fertilizer is also beneficial for increasing the yield of Ligusticum chuanxiong and accumulating its effective components, thus possessing significant production application value.

[0053] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for preparing a high-molecular nitrogen compound microbial liquid fertilizer for the cultivation of Ligusticum chuanxiong, characterized in that, Includes the following steps: (1) Mix brown sugar, edible vinegar, soybean meal and water to obtain a fermentation substrate; then inoculate the fermentation substrate with a compound microbial community, carry out anaerobic fermentation under closed conditions, and obtain a fermentation compound microbial liquid after filtration. (2) Add ammonia to formaldehyde and stir to adjust the pH to 7.5-8. After heating, add urea to react. Then add citric acid to adjust the pH to 5.8-6.

2. Then add potassium dihydrogen phosphate, magnesium sulfate and ferrous sulfate to react. Cool to room temperature to obtain urea-formaldehyde high molecular weight liquid compound fertilizer. (3) Mix the fermentation compound microbial liquid obtained in step (1), trehalose and glycine betaine to obtain a compound microbial strain protection solution; (4) Mix the compound microbial protection liquid obtained in step (3) with the first portion of the urea-formaldehyde polymer liquid compound fertilizer obtained in step (2) evenly, and then add the second portion of the urea-formaldehyde polymer liquid compound fertilizer obtained in step (2) and mix evenly to obtain the polymer nitrogen compound microbial liquid fertilizer for Ligusticum chuanxiong cultivation.

2. The preparation method of the high-molecular nitrogen compound microbial liquid fertilizer for Ligusticum chuanxiong cultivation as described in claim 1, characterized in that, In step (1), the mass ratio of brown sugar, edible vinegar, soybean meal and water is 5-30:1-10:1-5:

100.

3. The preparation method of the high-molecular nitrogen compound microbial liquid fertilizer for Ligusticum chuanxiong cultivation as described in claim 1, characterized in that, In step (1), the complex microbial community includes yeast, lactic acid bacteria and acetic acid bacteria.

4. The preparation method of the high-molecular nitrogen compound microbial liquid fertilizer for Ligusticum chuanxiong cultivation as described in claim 1, characterized in that, In step (1), anaerobic fermentation is carried out at a temperature of 28-32℃ for 15-20 days.

5. The preparation method of the high-molecular nitrogen compound microbial liquid fertilizer for Ligusticum chuanxiong cultivation as described in claim 1, characterized in that, In step (2), the mass ratio of formaldehyde, urea, potassium dihydrogen phosphate, magnesium sulfate and ferrous sulfate is 10-20:20-60:5-15:0.5-5:0.5-5.

6. The preparation method of the high-molecular nitrogen compound microbial liquid fertilizer for Ligusticum chuanxiong cultivation as described in claim 1, characterized in that, In step (3), the mass ratio of the fermentation compound microbial liquid, trehalose and glycine betaine is 100:1-10:1-10.

7. The preparation method of the high-molecular nitrogen compound microbial liquid fertilizer for Ligusticum chuanxiong cultivation as described in claim 1, characterized in that, In step (4), the mass ratio of the compound microbial inoculum protection liquid, the first portion of urea-formaldehyde polymer liquid compound fertilizer, and the second portion of urea-formaldehyde polymer liquid compound fertilizer is 100:100:200-1000.

8. The high-molecular nitrogen compound microbial liquid fertilizer for Ligusticum chuanxiong cultivation prepared by the method of any one of claims 1-7.

9. The application of the high molecular weight nitrogen compound microbial liquid fertilizer for Ligusticum chuanxiong cultivation as described in claim 8 in the cultivation of Ligusticum chuanxiong.

10. A method for cultivating Ligusticum chuanxiong, characterized in that, When cultivating Ligusticum striatum, apply the high molecular weight nitrogen compound microbial liquid fertilizer for Ligusticum striatum cultivation as described in claim 8; Specifically, apply 5-7 kg / mu when the plant has two leaves and one bud, 8-12 kg / mu after an interval of 6-8 days, 13-17 kg / mu during the stem and leaf development stage, 18-20 kg / mu during the second stem and leaf development stage, and 13-17 kg / mu one month before the root and stem enlargement stage; dilute 80-120 times when applying; apply by root irrigation.

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