Tea leaf theanine composition with anti-fatigue effect and preparation method thereof

Abstract

The invention relates to the technical field of health food, and particularly discloses a tea leaf theanine composition with an anti-fatigue effect and a preparation method thereof, and the tea leaf theanine composition comprises the following components: tea leaf theanine, gamma-aminobutyric acid, a plant extract, coenzyme Q10, taurine, compound B vitamins, a natural antioxidant and a functional carrier. The preparation method of the tea leaf theanine composition comprises the following steps: (1) preparing the composite plant extract; (2) preparing embedding powder; and (3) mixing the compound plant extract, the embedding powder, the tea leaf theanine, gamma-aminobutyric acid, taurine, compound B vitamins and a natural antioxidant, adding an adhesive, carrying out wet granulation, drying, granulating, and screening to obtain the tea leaf theanine composition with the anti-fatigue effect. The tea leaf theanine composition prepared by the invention shows remarkable anti-fatigue activity, and can realize an anti-fatigue effect by increasing energy reserve, reducing metabolic waste accumulation and enhancing antioxidant capacity.

Description

Technical Field

[0001] This application relates to the field of health food technology, and more specifically, it relates to a tea theanine composition with anti-fatigue effect and its preparation method. Background Technology

[0002] With the fast pace of modern life and increased work pressure, chronic fatigue syndrome has become a prevalent sub-health problem worldwide, manifesting as multi-dimensional symptoms such as lethargy, decreased physical strength, and poor concentration, which seriously affect quality of life and work efficiency. As a result, the demand for anti-fatigue products continues to grow.

[0003] Theanine, a natural amino acid unique to tea, can cross the blood-brain barrier to regulate GABA and dopamine levels, relieving mental fatigue and maintaining a relaxed state. However, when used alone, it targets only a single point of action, resulting in insufficient improvement of fatigue caused by physical exertion or oxidative stress. Furthermore, its bioavailability is low, as it is easily destroyed by stomach acid or rapidly metabolized. Moreover, most publicly available anti-fatigue compositions focus on a single dimension of action. For example, combinations of tea theanine with herbal extracts emphasize immune regulation, combinations with turmeric and grape seed powder emphasize metabolic regulation, and combinations with GABA and anthocyanins emphasize fatigue relief. None of these combinations achieve multi-target coverage of "neural regulation-energy metabolism-antioxidant-energy replenishment," and the synergistic effect is not fully realized. In addition, while some products employ single-component encapsulation technology to improve stability, this does not address the compatibility issues of components with different polarities. Fat-soluble and water-soluble components are prone to oxidation and inactivation when mixed. Furthermore, extraction processes often utilize traditional solvent extraction, resulting in low purity of theanine in tea. Anti-fatigue products often contain stimulants like caffeine, which, while providing short-term alertness, can lead to anxiety, insomnia, and dependence with long-term use, failing to meet the long-term needs of individuals with chronic fatigue. Based on the above, developing a novel, multi-target synergistic, and highly bioavailable tea theanine anti-fatigue composition represents a key technological gap in the current functional food field. Summary of the Invention

[0004] To address the technical problems mentioned in the background section, this application provides a tea theanine composition with anti-fatigue effects and a method for its preparation.

[0005] This application provides a tea theanine composition with anti-fatigue effect, using the following technical solution: A tea-based theanine composition with anti-fatigue effects comprises the following components in parts by weight: 80-150 parts tea theanine, 30-60 parts γ-aminobutyric acid, 80-100 parts plant extract, and 5-15 parts coenzyme Q10. 10 50-90 parts taurine, 3-8 parts B vitamins, 0.2-3 parts natural antioxidants, and 100-180 parts functional carriers.

[0006] Preferably, the plant extracts include maca extract, codonopsis extract, astragalus extract, and polygonatum powder.

[0007] Preferably, the plant extract is composed of maca extract, codonopsis extract, astragalus extract and polygonatum powder in a mass ratio of 25-45:18-32:12-22:10-18.

[0008] Preferably, the preparation method of the maca extract is as follows: take dried maca, crush and sieve it, add 60-70% ethanol aqueous solution, and extract it by ultrasonication 2-4 times at a temperature of 65-75℃, each time for 1.5-2.5h. Combine the extracts, filter, concentrate under reduced pressure, purify, and freeze dry under vacuum to obtain maca extract.

[0009] Preferably, the complex B vitamins consist of vitamin B1, vitamin B6, and vitamin B complex in a mass ratio of 1-1.2:1.5-2.5:0.8-1.2:3-5. 12 It is composed of nicotinamide.

[0010] Preferably, the natural antioxidant is composed of wolfberry powder, astaxanthin, and black wolfberry powder in a mass ratio of 5-20:1-5:1-3.

[0011] Preferably, the functional carrier is selected from two or more of maltodextrin, microcrystalline cellulose, fructooligosaccharides, and mannitol.

[0012] This application also provides a method for preparing a tea theanine composition with anti-fatigue effects, using the following technical solution: A method for preparing a tea theanine composition with anti-fatigue effects includes the following preparation steps: (1) Add the plant extract to purified water, stir at 85-95℃ for 1.5-2 hours, filter and collect the filtrate, concentrate under reduced pressure, freeze dry under vacuum to obtain the compound plant extract; (2) Coenzyme Q 10 Mix with a functional carrier, add purified water, stir and embed for 1-1.5 hours at 50-60℃, then spray dry to obtain the embedded powder; (3) Mix the compound plant extract, encapsulation powder, tea theanine, γ-aminobutyric acid, taurine, compound B vitamins and natural antioxidants. Mix for 25-35 minutes at a temperature of 28-32℃ and a speed of 30-40 rpm. Add binder and wet granulate. After granulation, dry at 45-55℃ until the moisture content is ≤5%. After granulation and sieving, obtain the tea theanine composition with anti-fatigue effect.

[0013] Preferably, in step (2), the inlet air temperature of the spray drying is 170-175℃ and the outlet air temperature is 75-80℃.

[0014] Preferably, in step (3), the adhesive is composed of hydroxypropyl methylcellulose, xylooligosaccharides and ultrapure water in a mass ratio of 1-1.5:0.8-2:20-30.

[0015] In summary, this application has the following beneficial effects: This application utilizes a compound of maca extract, codonopsis extract, astragalus extract, polygonatum powder, and tea leaves containing theanine, γ-aminobutyric acid, taurine, and coenzyme Q. 10 Including B vitamins, a multi-target synergistic energy metabolism regulatory network is constructed, enabling the composition to exert its effects from multiple dimensions such as energy synthesis, metabolic regulation, and functional protection, synergistically activating the body's energy metabolism pathways: Maca amide and macaene in maca extract can promote the regulation of the hypothalamic-pituitary-adrenal axis function and improve energy metabolism efficiency; Codonopsis polysaccharides in Codonopsis extract, astragaloside A in Astragalus extract, and Polygonatum polysaccharides in Polygonatum powder can synergistically promote the activation of hepatocyte glycogen synthase, accelerate the synthesis and storage of liver glycogen and muscle glycogen, provide a continuous and stable energy supply for the body's exercise, and avoid fatigue caused by rapid energy depletion; theanine in tea, as a central nervous system regulator, can synergistically work with γ-aminobutyric acid to alleviate excessive excitation of the central nervous system during exercise, reduce mental fatigue, and regulate neuro-humoral balance, reducing exercise stress response; taurine, as an important sulfur-containing amino acid in the body, can participate in glycolysis and fat metabolism, improve energy conversion efficiency, and reduce the accumulation of intermediate metabolites such as lactic acid; Coenzyme Q10... 10As a key component of the mitochondrial respiratory chain, it can enhance mitochondrial oxidative phosphorylation function and improve cellular energy production levels. Complex B vitamins participate in the metabolism of carbohydrates, proteins, and fats as coenzymes, ensuring the efficient decomposition and utilization of various nutrients, reducing the accumulation of metabolic wastes such as serum urea nitrogen after exercise, and significantly enhancing the body's tolerance to exercise load, prolonging the weighted swimming time in mice. It synergistically delays fatigue by improving energy supply, metabolic efficiency, and stress regulation. By combining natural antioxidants composed of wolfberry powder, astaxanthin, and black wolfberry powder with the above active ingredients, a complementary and synergistic oxidative protection system is formed, enabling the composition to synergistically enhance the activity of total superoxide dismutase (T-SOD) in liver tissue: wolfberry powder has a strong free radical scavenging ability, directly capturing reactive oxygen species such as hydroxyl radicals and superoxide anions generated during exercise, reducing oxidative stress damage to cells; astaxanthin, as a fat-soluble antioxidant, can penetrate cell membranes and mitochondrial membranes, targeting and eliminating free radicals generated during lipid peroxidation, protecting the integrity of biological membrane structures, and... It effectively inhibits lipid peroxidation; black goji berry powder contains water-soluble antioxidants that can function in extracellular fluid and cytoplasm, forming a comprehensive antioxidant network with astaxanthin, covering different sites and stages of free radical production; when these three components work synergistically with tea theanine, astragaloside A, etc., they can not only directly scavenge free radicals, but also enhance the body's own antioxidant defense system by upregulating the expression of endogenous antioxidant enzymes such as T-SOD, reducing the damage of oxidative stress to liver cells and muscle cells, avoiding the decline in energy metabolism efficiency caused by impaired cell function, and further enhancing anti-fatigue effects. In addition, the various components of natural antioxidants complement each other in terms of mechanism of action and site of action, effectively avoiding the problems of limited target and narrow antioxidant spectrum of single antioxidant components, making the antioxidant effect more comprehensive and longer-lasting. Ultimately, through the energy metabolism regulation of plant extracts and active ingredients, and the oxidative protection of natural antioxidants, the composition forms a triple anti-fatigue synergistic system of "energy replenishment - metabolic regulation - oxidative protection," which significantly enhances the anti-fatigue effect of the composition. Its intensity and persistence far exceed those of single-component or partially compounded systems. Detailed Implementation

[0016] The present application will be further described in detail below with reference to the embodiments.

[0017] The maca used in the embodiments and comparative examples of this application was purchased from Jilin Yilutang Trading Co., Ltd.; the Codonopsis pilosula extract and Astragalus membranaceus extract were purchased from Zhejiang Yicun Biotechnology Co., Ltd.; Polygonatum sibiricum powder, black goji berry powder and goji berry powder were provided by Hunan Jinong Bio-resources Co., Ltd.; astaxanthin was purchased from Shaanxi Longbeiyuan Bioengineering Co., Ltd.; hydroxypropyl methylcellulose (food grade) was purchased from Jiangsu Caiwei Biotechnology Co., Ltd.; xylooligosaccharides were purchased from Hebei Hongtao Bioengineering Co., Ltd.

[0018] Examples 1-3 provide a tea theanine composition with anti-fatigue effect and its preparation method.

[0019] Example 1 A tea-based theanine composition with anti-fatigue effects comprises the following components in parts by weight: 80 parts tea theanine, 30 parts γ-aminobutyric acid, 80 parts plant extract, and 5 parts coenzyme Q. 10 The formula contains 50 parts taurine, 3 parts B-complex vitamins, 0.2 parts natural antioxidants, and 100 parts functional carriers. The plant extracts consist of maca extract, codonopsis extract, astragalus extract, and polygonatum powder in a mass ratio of 25:18:12:10. The B-complex vitamins consist of vitamin B1, vitamin B6, and vitamin B complex in a mass ratio of 1:1.5:0.8:3. 12 It is composed of nicotinamide, and the natural antioxidant is composed of wolfberry powder, astaxanthin and black wolfberry powder in a mass ratio of 5:1:1. The functional carrier is composed of maltodextrin and microcrystalline cellulose in a mass ratio of 1:2.

[0020] The preparation method of maca extract is as follows: dried maca is pulverized and passed through a 100-mesh sieve, and 60% ethanol aqueous solution is added. The solid-liquid mass ratio is controlled at 1:10. The maca is extracted twice at 65℃ with an ultrasonic power of 100W and an ultrasonic frequency of 40KHz, each time for 1.5h. The extracts are combined, filtered, and concentrated under reduced pressure at a vacuum degree of 0.06MPa for 2h. After purification, the maca extract is freeze-dried at -40℃ for 10h to obtain maca extract.

[0021] A method for preparing a tea theanine composition with anti-fatigue effects includes the following preparation steps: (1) Add the plant extract to purified water, control the solid-liquid mass ratio to 1:10, stir at 300 rpm for 1.5 h at 85 ℃, filter and collect the filtrate, concentrate under reduced pressure for 4 h at 0.06 MPa, freeze dry at -40 ℃ for 12 h to obtain the compound plant extract. (2) Coenzyme Q 10 Mix with a functional carrier, add purified water, control the solid-liquid mass ratio to 1:8, stir and embed for 1 hour at 50℃ and 200 rpm, spray dry to obtain embedded powder, control the inlet air temperature of spray drying to 170℃ and the outlet air temperature to 75℃. (3) The compound plant extract, encapsulation powder, tea theanine, γ-aminobutyric acid, taurine, compound B vitamins and natural antioxidants are mixed and mixed for 25 minutes at 28°C and 30 rpm to obtain a mixture. A binder is added to the mixture, and wet granulation is performed. After granulation, the mixture is dried at 45°C until the moisture content is 5%. After granulation and sieving, a tea theanine composition with anti-fatigue effect is obtained. The binder is composed of hydroxypropyl methylcellulose, xylooligosaccharide and ultrapure water in a mass ratio of 1:0.8:20. The amount of binder added is 5% of the mass of the mixture.

[0022] Example 2 A tea-based theanine composition with anti-fatigue effects comprises the following components in parts by weight: 120 parts tea theanine, 45 parts γ-aminobutyric acid, 90 parts plant extract, and 10 parts coenzyme Q. 10 The formula contains 70 parts taurine, 5 parts B-complex vitamins, 1.5 parts natural antioxidants, and 140 parts functional carriers. The plant extracts consist of maca extract, codonopsis extract, astragalus extract, and polygonatum powder in a mass ratio of 35:24:18:14. The B-complex vitamins consist of vitamin B1, vitamin B6, and vitamin B complex in a mass ratio of 1.1:2:1:4. 12 It is composed of nicotinamide, and the natural antioxidant is composed of wolfberry powder, astaxanthin and black wolfberry powder in a mass ratio of 12:3:2. The functional carrier is composed of maltodextrin and microcrystalline cellulose in a mass ratio of 1.5:2.5.

[0023] The preparation method of maca extract is as follows: dried maca is pulverized and passed through a 150-mesh sieve, and 65% ethanol aqueous solution is added. The solid-liquid mass ratio is controlled at 2:11. The maca is extracted three times at 70℃ with an ultrasonic power of 150W and an ultrasonic frequency of 50KHz, each time for 2 hours. The extracts are combined, filtered, and concentrated under reduced pressure at a vacuum degree of 0.09MPa for 4 hours. After purification, the maca extract is freeze-dried at -40℃ for 12 hours to obtain maca extract.

[0024] A method for preparing a tea theanine composition with anti-fatigue effects includes the following preparation steps: (1) Add the plant extract to purified water, control the solid-liquid mass ratio to 1.5:10, stir at 90℃ for 1.8h with a stirring speed of 400rpm, filter and collect the filtrate, concentrate under reduced pressure for 4h at a vacuum degree of 0.09MPa, and freeze dry at -50℃ for 12h to obtain the compound plant extract. (2) Coenzyme Q 10Mix with a functional carrier, add purified water, control the solid-liquid mass ratio at 2:9, stir and embed for 1.2 hours at 55℃ and 300 rpm, spray dry to obtain embedded powder, control the inlet air temperature of spray drying at 172℃ and the outlet air temperature at 78℃. (3) The compound plant extract, encapsulation powder, tea theanine, γ-aminobutyric acid, taurine, compound B vitamins and natural antioxidants are mixed and mixed for 30 min at 30℃ and 35 rpm to obtain a mixture. A binder is added to the mixture, and wet granulation is performed. After granulation, the mixture is dried at 50℃ until the moisture content is 5%. After granulation and sieving, a tea theanine composition with anti-fatigue effect is obtained. The binder is composed of hydroxypropyl methylcellulose, xylooligosaccharide and ultrapure water in a mass ratio of 1.2:1:25. The amount of binder added is 7% of the mass of the mixture.

[0025] Example 3 A tea-based theanine composition with anti-fatigue effects comprises the following components in parts by weight: 150 parts tea theanine, 60 parts γ-aminobutyric acid, 100 parts plant extract, and 15 parts coenzyme Q. 10 The formula contains 90 parts taurine, 8 parts B-complex vitamins, 3 parts natural antioxidants, and 180 parts functional carriers. The plant extracts consist of maca extract, codonopsis extract, astragalus extract, and polygonatum powder in a mass ratio of 25-45:18-32:12-22:10-18. The B-complex vitamins consist of vitamin B1, vitamin B6, and vitamin B complex in a mass ratio of 1.2:2.5:1.2:5. 12 It is composed of nicotinamide, and the natural antioxidant is composed of wolfberry powder, astaxanthin and black wolfberry powder in a mass ratio of 20:5:3. The functional carrier is composed of maltodextrin and microcrystalline cellulose in a mass ratio of 2:3.

[0026] The preparation method of maca extract is as follows: dried maca is pulverized and passed through a 200-mesh sieve, and 70% ethanol aqueous solution is added. The solid-liquid mass ratio is controlled at 3:12. The mixture is ultrasonically extracted 4 times at 75℃ with an ultrasonic power of 200W and an ultrasonic frequency of 60KHz, each time for 2.5h. The extracts are combined, filtered, and concentrated under reduced pressure at 0.09MPa for 4h. After purification, the maca extract is freeze-dried at -40℃ for 12h to obtain maca extract. A method for preparing a tea theanine composition with anti-fatigue effects includes the following preparation steps: (1) Add the plant extract to purified water, control the solid-liquid mass ratio to 2:10, stir at 95℃ for 2 hours at a stirring speed of 500 rpm, filter and collect the filtrate, concentrate under reduced pressure for 4 hours at a vacuum degree of 0.06 MPa, and freeze dry at -40℃ for 12 hours to obtain the compound plant extract. (2) Coenzyme Q 10 Mix with a functional carrier, add purified water, control the solid-liquid mass ratio at 3:10, stir and embed for 1.5 hours at 60℃ and 400 rpm, spray dry to obtain embedded powder, control the inlet air temperature of spray drying at 175℃ and the outlet air temperature at 80℃. (3) The compound plant extract, encapsulation powder, tea theanine, γ-aminobutyric acid, taurine, compound B vitamins and natural antioxidants are mixed and mixed for 35 minutes at a temperature of 32℃ and a speed of 40 rpm to obtain a mixture. A binder is added to the mixture, and wet granulation is performed. After granulation, the mixture is dried at 55℃ until the moisture content is 5%. After granulation and sieving, a tea theanine composition with anti-fatigue effect is obtained. The binder is composed of hydroxypropyl methylcellulose, xylooligosaccharide and ultrapure water in a mass ratio of 1.5:2:30. The amount of binder added is 8% of the mass of the mixture.

[0027] Comparative Example 1 A tea-based theanine composition with anti-fatigue effects comprises the following components in parts by weight: 80 parts tea theanine, 30 parts γ-aminobutyric acid, 80 parts plant extract, and 5 parts coenzyme Q. 10 The formula contains 50 parts taurine, 3 parts B-complex vitamins, 0.2 parts natural antioxidants, and 100 parts functional carriers. The plant extracts consist of Codonopsis pilosula extract, Astragalus membranaceus extract, and Polygonatum sibiricum powder in a mass ratio of 18:12:10. The B-complex vitamins consist of vitamin B1, vitamin B6, and vitamin B1 in a mass ratio of 1:1.5:0.8:3. 12 It is composed of nicotinamide, and the natural antioxidant is composed of wolfberry powder, astaxanthin and black wolfberry powder in a mass ratio of 5:1:1. The functional carrier is composed of maltodextrin and microcrystalline cellulose in a mass ratio of 1:2.

[0028] A method for preparing a tea theanine composition with anti-fatigue effects includes the following preparation steps: (1) Add the plant extract to purified water, control the solid-liquid mass ratio to 1:10, stir at 300 rpm for 1.5 h at 85 ℃, filter and collect the filtrate, concentrate under reduced pressure for 4 h at 0.06 MPa, freeze dry at -40 ℃ for 12 h to obtain the compound plant extract. (2) Coenzyme Q 10Mix with a functional carrier, add purified water, control the solid-liquid mass ratio to 1:8, stir and embed for 1 hour at 50℃ and 200 rpm, spray dry to obtain embedded powder, control the inlet air temperature of spray drying to 170℃ and the outlet air temperature to 75℃. (3) The compound plant extract, encapsulation powder, tea theanine, γ-aminobutyric acid, taurine, compound B vitamins and natural antioxidants are mixed and mixed for 25 minutes at 28°C and 30 rpm to obtain a mixture. A binder is added to the mixture, and wet granulation is performed. After granulation, the mixture is dried at 45°C until the moisture content is 5%. After granulation and sieving, a tea theanine composition with anti-fatigue effect is obtained. The binder is composed of hydroxypropyl methylcellulose, xylooligosaccharide and ultrapure water in a mass ratio of 1:0.8:20. The amount of binder added is 5% of the mass of the mixture.

[0029] Comparative Example 2 A tea-based theanine composition with anti-fatigue effects comprises the following components in parts by weight: 80 parts tea theanine, 30 parts γ-aminobutyric acid, 80 parts plant extract, and 5 parts coenzyme Q. 10 The formula contains 50 parts taurine, 3 parts B-complex vitamins, 0.2 parts natural antioxidants, and 100 parts functional carriers. The plant extracts consist of maca extract, codonopsis extract, astragalus extract, and polygonatum powder in a mass ratio of 25:18:12:10. The B-complex vitamins consist of vitamin B1, vitamin B6, and vitamin B complex in a mass ratio of 1:1.5:0.8:3. 12 It is composed of nicotinamide, and the natural antioxidant is composed of astaxanthin and black goji berry powder in a mass ratio of 1:1. The functional carrier is composed of maltodextrin and microcrystalline cellulose in a mass ratio of 1:2.

[0030] The preparation method of maca extract is as follows: dried maca is pulverized and passed through a 100-mesh sieve, and 60% ethanol aqueous solution is added. The solid-liquid mass ratio is controlled at 1:10. The maca is extracted twice at 65℃ with an ultrasonic power of 100W and an ultrasonic frequency of 40KHz, each time for 1.5h. The extracts are combined, filtered, and concentrated under reduced pressure at a vacuum degree of 0.06MPa for 2h. After purification, the maca extract is freeze-dried at -40℃ for 10h to obtain maca extract.

[0031] A method for preparing a tea theanine composition with anti-fatigue effects includes the following preparation steps: (1) Add the plant extract to purified water, control the solid-liquid mass ratio to 1:10, stir at 300 rpm for 1.5 h at 85 ℃, filter and collect the filtrate, concentrate under reduced pressure for 4 h at 0.06 MPa, freeze dry at -40 ℃ for 12 h to obtain the compound plant extract. (2) Mix coenzyme Q10 with a functional carrier, add purified water, control the solid-liquid mass ratio to be 1:8, stir and embed for 1 hour at 50℃ and 200 rpm, spray dry to obtain embedded powder, control the inlet air temperature of spray drying to be 170℃ and the outlet air temperature to be 75℃. (3) The compound plant extract, encapsulation powder, tea theanine, γ-aminobutyric acid, taurine, compound B vitamins and natural antioxidants are mixed and mixed for 25 minutes at 28°C and 30 rpm to obtain a mixture. A binder is added to the mixture, and wet granulation is performed. After granulation, the mixture is dried at 45°C until the moisture content is 5%. After granulation and sieving, a tea theanine composition with anti-fatigue effect is obtained. The binder is composed of hydroxypropyl methylcellulose, xylooligosaccharide and ultrapure water in a mass ratio of 1:0.8:20. The amount of binder added is 5% of the mass of the mixture.

[0032] Comparative Example 3 A tea-based theanine composition with anti-fatigue effects comprises the following components in parts by weight: 80 parts tea theanine, 30 parts γ-aminobutyric acid, 80 parts plant extract, and 5 parts coenzyme Q. 10 The formula contains 50 parts taurine, 3 parts B-complex vitamins, 0.2 parts natural antioxidants, and 100 parts functional carriers. The plant extracts consist of maca extract, codonopsis extract, astragalus extract, and polygonatum powder in a mass ratio of 25:18:12:10. The B-complex vitamins consist of vitamin B1, vitamin B6, and vitamin B complex in a mass ratio of 1:1.5:0.8:3. 12 It consists of nicotinamide, and the natural antioxidant is composed of wolfberry powder and black wolfberry powder in a mass ratio of 5:1. The functional carrier is composed of maltodextrin and microcrystalline cellulose in a mass ratio of 1:2.

[0033] The preparation method of maca extract is as follows: dried maca is pulverized and passed through a 100-mesh sieve, and 60% ethanol aqueous solution is added. The solid-liquid mass ratio is controlled at 1:10. The maca is extracted twice at 65℃ with an ultrasonic power of 100W and an ultrasonic frequency of 40KHz, each time for 1.5h. The extracts are combined, filtered, and concentrated under reduced pressure at a vacuum degree of 0.06MPa for 2h. After purification, the maca extract is freeze-dried at -40℃ for 10h to obtain maca extract.

[0034] A method for preparing a tea theanine composition with anti-fatigue effects includes the following preparation steps: (1) Add the plant extract to purified water, control the solid-liquid mass ratio to 1:10, stir at 300 rpm for 1.5 h at 85 ℃, filter and collect the filtrate, concentrate under reduced pressure for 4 h at 0.06 MPa, freeze dry at -40 ℃ for 12 h to obtain the compound plant extract. (2) Coenzyme Q 10 Mix with a functional carrier, add purified water, control the solid-liquid mass ratio to 1:8, stir and embed for 1 hour at 50℃ and 200 rpm, spray dry to obtain embedded powder, control the inlet air temperature of spray drying to 170℃ and the outlet air temperature to 75℃. (3) The compound plant extract, encapsulation powder, tea theanine, γ-aminobutyric acid, taurine, compound B vitamins and natural antioxidants are mixed and mixed for 25 minutes at 28°C and 30 rpm to obtain a mixture. A binder is added to the mixture, and wet granulation is performed. After granulation, the mixture is dried at 45°C until the moisture content is 5%. After granulation and sieving, a tea theanine composition with anti-fatigue effect is obtained. The binder is composed of hydroxypropyl methylcellulose, xylooligosaccharide and ultrapure water in a mass ratio of 1:0.8:20. The amount of binder added is 5% of the mass of the mixture.

[0035] Comparative Example 4 A tea-based theanine composition with anti-fatigue effects comprises the following components in parts by weight: 80 parts tea theanine, 30 parts γ-aminobutyric acid, 80 parts plant extract, and 5 parts coenzyme Q. 10 The formula contains 50 parts taurine, 3 parts B-complex vitamins, 0.2 parts natural antioxidants, and 100 parts functional carriers. The plant extracts consist of maca extract, codonopsis extract, astragalus extract, and polygonatum powder in a mass ratio of 25:18:12:10. The B-complex vitamins consist of vitamin B1, vitamin B6, and vitamin B complex in a mass ratio of 1:1.5:0.8:3. 12 It consists of nicotinamide, natural antioxidants composed of goji berry powder and astaxanthin in a mass ratio of 5:1, and functional carriers composed of maltodextrin and microcrystalline cellulose in a mass ratio of 1:2.

[0036] The preparation method of maca extract is as follows: dried maca is pulverized and passed through a 100-mesh sieve, and 60% ethanol aqueous solution is added. The solid-liquid mass ratio is controlled at 1:10. The maca is extracted twice at 65℃ with an ultrasonic power of 100W and an ultrasonic frequency of 40KHz, each time for 1.5h. The extracts are combined, filtered, and concentrated under reduced pressure at a vacuum degree of 0.06MPa for 2h. After purification, the maca extract is freeze-dried at -40℃ for 10h to obtain maca extract.

[0037] A method for preparing a tea theanine composition with anti-fatigue effects includes the following preparation steps: (1) Add the plant extract to purified water, control the solid-liquid mass ratio to 1:10, stir at 300 rpm for 1.5 h at 85 ℃, filter and collect the filtrate, concentrate under reduced pressure for 4 h at 0.06 MPa, freeze dry at -40 ℃ for 12 h to obtain the compound plant extract. (2) Coenzyme Q 10 Mix with a functional carrier, add purified water, control the solid-liquid mass ratio to 1:8, stir and embed for 1 hour at 50℃ and 200 rpm, spray dry to obtain embedded powder, control the inlet air temperature of spray drying to 170℃ and the outlet air temperature to 75℃. (3) The compound plant extract, encapsulation powder, tea theanine, γ-aminobutyric acid, taurine, compound B vitamins and natural antioxidants are mixed and mixed for 25 minutes at 28°C and 30 rpm to obtain a mixture. A binder is added to the mixture, and wet granulation is performed. After granulation, the mixture is dried at 45°C until the moisture content is 5%. After granulation and sieving, a tea theanine composition with anti-fatigue effect is obtained. The binder is composed of hydroxypropyl methylcellulose, xylooligosaccharide and ultrapure water in a mass ratio of 1:0.8:20. The amount of binder added is 5% of the mass of the mixture.

[0038] Comparative Example 5 A tea-based theanine composition with anti-fatigue effects comprises the following components in parts by weight: 80 parts tea theanine, 30 parts γ-aminobutyric acid, 80 parts plant extract, and 5 parts coenzyme Q. 10 The formula consists of 50 parts taurine, 3 parts complex B vitamins, 0.2 parts natural antioxidants, and 100 parts functional carriers. The plant extracts are composed of maca extract, codonopsis extract, astragalus extract, and polygonatum powder in a mass ratio of 25:18:12:10. The complex B vitamins are composed of vitamin B1, vitamin B6, and niacinamide in a mass ratio of 1:1.5:3. The natural antioxidants are composed of wolfberry powder, astaxanthin, and black wolfberry powder in a mass ratio of 5:1:1. The functional carriers are composed of maltodextrin and microcrystalline cellulose in a mass ratio of 1:2.

[0039] The preparation method of maca extract is as follows: dried maca is pulverized and passed through a 100-mesh sieve, and 60% ethanol aqueous solution is added. The solid-liquid mass ratio is controlled at 1:10. The maca is extracted twice at 65℃ with an ultrasonic power of 100W and an ultrasonic frequency of 40KHz, each time for 1.5h. The extracts are combined, filtered, and concentrated under reduced pressure at a vacuum degree of 0.06MPa for 2h. After purification, the maca extract is freeze-dried at -40℃ for 10h to obtain maca extract.

[0040] A method for preparing a tea theanine composition with anti-fatigue effects includes the following preparation steps: (1) Add the plant extract to purified water, control the solid-liquid mass ratio to 1:10, stir at 300 rpm for 1.5 h at 85 ℃, filter and collect the filtrate, concentrate under reduced pressure for 4 h at 0.06 MPa, freeze dry at -40 ℃ for 12 h to obtain the compound plant extract. (2) Coenzyme Q 10 Mix with a functional carrier, add purified water, control the solid-liquid mass ratio to 1:8, stir and embed for 1 hour at 50℃ and 200 rpm, spray dry to obtain embedded powder, control the inlet air temperature of spray drying to 170℃ and the outlet air temperature to 75℃. (3) The compound plant extract, encapsulation powder, tea theanine, γ-aminobutyric acid, taurine, compound B vitamins and natural antioxidants are mixed and mixed for 25 minutes at 28°C and 30 rpm to obtain a mixture. A binder is added to the mixture, and wet granulation is performed. After granulation, the mixture is dried at 45°C until the moisture content is 5%. After granulation and sieving, a tea theanine composition with anti-fatigue effect is obtained. The binder is composed of hydroxypropyl methylcellulose, xylooligosaccharide and ultrapure water in a mass ratio of 1:0.8:20. The amount of binder added is 5% of the mass of the mixture.

[0041] Performance testing The anti-fatigue activity of the tea theanine compositions prepared in Examples 1-3 and Comparative Examples 1-5 was evaluated by weight-bearing swimming tests in mice and biomarker detection.

[0042] One hundred and eight healthy female ICR mice of SPF grade, six weeks old, weighing 18-22g, were selected and acclimatized for seven days. During this period, they were allowed free access to food and water. The rearing environment was kept cool and well-ventilated, with a humidity of 50-60% and a room temperature of 25±2℃.

[0043] Reagents and kits: Serum urea nitrogen (BUN) assay kit, liver glycogen (LG) assay kit, muscle glycogen (MG) assay kit, total superoxide dismutase (T-SOD) assay kit (purchased from a biotechnology company); physiological saline (self-made, 0.9% NaCl); anhydrous ethanol, potassium dihydrogen phosphate and other analytical grade reagents.

[0044] Instruments and equipment: constant temperature swimming bath (size: 60cm×40cm×50cm), electronic balance (accuracy 0.1mg), high-speed refrigerated centrifuge, ultraviolet spectrophotometer, tissue homogenizer, constant temperature water bath, etc.

[0045] Experimental Methods (I) Animal Grouping and Drug Administration After the adaptive feeding period, 108 mice were randomly divided into two groups of 54 each by pre-swimming screening (those that could swim continuously for more than 5 minutes were considered qualified): Group A (weighted swimming test group): 54 mice, randomly divided into 9 subgroups (6 mice in each subgroup), corresponding to Examples 1-3, Comparative Examples 1-5 and the blank control group respectively; Group B (bioindicator detection group): 54 mice, grouped in the same way as Group A.

[0046] Administration: All test samples were dissolved or diluted with physiological saline and administered to mice by gavage at a dose of 200 mg / kg·d twice daily for 30 consecutive days; the blank control was administered by gavage with an equal volume of physiological saline.

[0047] Group A (weighted swimming test): 30 minutes after the last administration, the weighted swimming test was conducted: a lead block (10% of the mouse's body weight) was fixed at 1 / 3 of the mouse's tail; the mouse was placed in a constant temperature swimming tank with a water depth of 40 cm and a water temperature of 25±2℃, and was allowed to swim freely; the time from the start of swimming to the time when the mouse could not float to the surface on its own within 8 seconds after being completely submerged in the water was recorded as the weighted swimming time.

[0048] Group B (Biometrics Detection): 30 minutes after the last administration, the following procedures were performed: Mice were placed in the aforementioned swimming tank and swam freely for 30 minutes, followed by 60 minutes of rest. Mice were anesthetized with ether, and blood was collected from the orbital venous plexus. The blood was centrifuged at 3500 rpm for 15 minutes, and the serum was separated and stored at -20°C for later use. Liver tissue was dissected, rinsed with physiological saline, and dried. Physiological saline was added at a mass-to-volume ratio of 1:9, and a 10% liver homogenate was prepared using a tissue homogenizer. The homogenate was centrifuged at 8000 rpm for 10 minutes at 4°C, and the supernatant was collected. Following the instructions of each kit, the serum urea nitrogen (BUN) content, liver glycogen (LG) content, muscle glycogen (MG) content, and total superoxide dismutase (T-SOD) activity were measured. Data analysis was performed using SPSS 22.0 statistical software. Results are expressed as mean ± standard deviation (x ± s). One-way ANOVA was used for comparisons between groups, and P < 0.05 was considered statistically significant.

[0049] Table 1. Effects of tea-theanine composition on anti-fatigue related indicators in mice (x±s, n=6)

[0050] As shown in Table 1, the tea theanine composition prepared in this application can significantly increase the swimming time of mice under load. The urea nitrogen content in the example group was significantly lower than that in the comparative group, indicating that the composition can reduce the accumulation of urea nitrogen in the body after exercise and alleviate fatigue. The liver glycogen and muscle glycogen content in the example group were significantly higher than those in the comparative group, indicating that the composition can increase energy reserves and delay the onset of fatigue. The T-SOD activity in the example group was significantly higher than that in the comparative group, indicating that the composition can enhance the body's antioxidant capacity, reduce free radical damage, and exert an anti-fatigue effect. The tea theanine composition prepared in this application all exhibit significant anti-fatigue activity, and can achieve anti-fatigue effects by increasing energy reserves, reducing the accumulation of metabolic waste, and enhancing antioxidant capacity.

[0051] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A tea theanine composition with anti-fatigue effects, characterized in that, The product comprises the following components by weight: 80-150 parts tea theanine, 30-60 parts γ-aminobutyric acid, 80-100 parts plant extracts, and 5-15 parts coenzyme Q. 10 50-90 parts taurine, 3-8 parts B vitamins, 0.2-3 parts natural antioxidants, and 100-180 parts functional carriers.

2. The tea theanine composition with anti-fatigue effect according to claim 1, characterized in that, The plant extracts include maca extract, codonopsis extract, astragalus extract, and polygonatum powder.

3. The tea theanine composition with anti-fatigue effect according to claim 2, characterized in that, The plant extract is composed of maca extract, codonopsis extract, astragalus extract and polygonatum powder in a mass ratio of 25-45:18-32:12-22:10-18.

4. The tea theanine composition with anti-fatigue effect according to claim 2, characterized in that, The preparation method of the maca extract is as follows: take dried maca, crush and sieve it, add 60-70% ethanol aqueous solution, and extract it by ultrasonication 2-4 times at a temperature of 65-75℃, each time for 1.5-2.5h. Combine the extracts, concentrate under reduced pressure, purify, and freeze dry under vacuum to obtain maca extract.

5. The tea theanine composition with anti-fatigue effect according to claim 1, characterized in that, The complex B vitamins consist of vitamin B1, vitamin B6, and vitamin B complex in a mass ratio of 1-1.2:1.5-2.5:0.8-1.2:3-5. 12 It is composed of nicotinamide.

6. The tea theanine composition with anti-fatigue effect according to claim 1, characterized in that, The natural antioxidant is composed of wolfberry powder, astaxanthin, and black wolfberry powder in a mass ratio of 5-20:1-5:1-3.

7. The tea theanine composition with anti-fatigue effect according to claim 1, characterized in that, The functional carrier is selected from two or more of maltodextrin, microcrystalline cellulose, fructooligosaccharides, and mannitol.

8. A method for preparing a tea theanine composition with anti-fatigue effect as described in any one of claims 1-7, characterized in that, The preparation steps include the following: (1) Add the plant extract to purified water, stir at 85-95℃ for 1.5-2 hours, filter and collect the filtrate, concentrate under reduced pressure, freeze dry under vacuum to obtain the compound plant extract; (2) Coenzyme Q 10 Mix with a functional carrier, add purified water, stir and embed for 1-1.5 hours at 50-60℃, then spray dry to obtain the embedded powder; (3) Mix the compound plant extract, encapsulation powder, tea theanine, γ-aminobutyric acid, taurine, compound B vitamins and natural antioxidants. Mix for 25-35 minutes at a temperature of 28-32℃ and a speed of 30-40 rpm. Add binder and wet granulate. After granulation, dry at 45-55℃ until the moisture content is ≤5%. After granulation and sieving, obtain the tea theanine composition with anti-fatigue effect.

9. The method for preparing the tea theanine composition with anti-fatigue effect according to claim 8, characterized in that, In step (2), the inlet air temperature of the spray dryer is 170-175℃ and the outlet air temperature is 75-80℃.

10. The method for preparing the tea theanine composition with anti-fatigue effect according to claim 8, characterized in that, In step (3), the adhesive is composed of hydroxypropyl methylcellulose, xylooligosaccharides and ultrapure water in a mass ratio of 1-1.5:0.8-2:20-30.

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