Small fluorescent microscopic imaging device

Abstract

The invention discloses a small fluorescent microscopic imaging device, which comprises a box body assembly, a glass slide moving assembly, an objective lens assembly, a dichroscope assembly, a reflecting prism, a light shield assembly, a tube lens, a camera and a multicolor LED light source assembly, the glass slide moving assembly is mounted at the upper part of the box body assembly, the objective lens assembly is mounted below the glass slide moving assembly, the dichroscope assembly is arranged below an objective lens in the objective lens assembly, and the reflecting prism is arranged below the dichroscope assembly; the multi-color LED light source assembly is arranged on the side face of the dichroscope assembly and fixedly connected with a side plate of the box body assembly. A camera, a light shield assembly and a tube lens are sequentially arranged on a bottom plate of the box body assembly, a light path is parallel to a light path of the LED light source assembly, the camera is in threaded connection with the light shield assembly, and the end face of the light shield assembly is always in contact with the end face of the tube lens; compared with the prior art, the micro-imaging device has the advantages that the imaging range can be expanded to the greatest extent while the micro-imaging device is miniaturized.

Description

Technical Field

[0001] This invention belongs to the field of medical optics, specifically a small fluorescence microscopy imaging device. Background Technology

[0002] Miniaturized, flexible, and portable microscopic imaging devices allow for sample observation anytime, anywhere. Currently, most microscopic devices are fixed and inconvenient to move, limiting their application primarily to laboratories. While some small microscopic imaging devices exist, their size restricts observation to a small portion of the slide, making it difficult to cover the entire coverslip. This invention addresses these issues by proposing a miniaturized microscopic imaging device. Summary of the Invention

[0003] The purpose of this invention is to provide a small fluorescence microscopy imaging device that can maximize the imaging range while miniaturizing the microscopy imaging device.

[0004] The technical solution of this invention is as follows: a small fluorescence microscopy imaging device, comprising a housing assembly, a slide moving assembly, an objective lens assembly, a dichroic mirror assembly, a reflecting prism, a light shield assembly, a tube mirror, a camera, and a multicolor LED light source assembly; the slide moving assembly is installed on the upper part of the housing assembly for placing and moving samples; the objective lens assembly is installed below the slide moving assembly; the dichroic mirror assembly is located below the objective lens in the objective lens assembly for selectively reflecting or transmitting light of a specific wavelength to achieve optical path control; the reflecting prism is located below the dichroic mirror assembly; the multicolor LED light source assembly is located on the side of the dichroic mirror assembly and is fixedly connected to the side plate of the housing assembly; the camera, light shield assembly, and tube mirror are sequentially arranged on the bottom plate of the housing assembly, with the optical path parallel to the optical path of the LED light source assembly; the distance between the camera and the light shield assembly is continuously adjusted by relative rotation; the end face of the light shield assembly is always in contact with the end face of the tube mirror.

[0005] Furthermore, the slide moving assembly includes: a cover plate, a slide platform, an elastic clamping assembly, a slide, a guide shaft support, a supporting side lead screw support, a first gear, a second gear, a coarse adjusting lead screw, a lead screw ball nut, a fine adjusting lead screw, a fixed side lead screw support, and a guide shaft. The slide platform consists of a bushing and a cover plate fixedly connected to the housing assembly. The slide is placed on the slide platform and pressed down by an elastic clamping assembly. A guide shaft support, a support-side lead screw support, and a fixed-side lead screw support are fixed to the cover plate. The central shaft of the first gear is fixedly connected to a coarse adjustment lead screw, and the central shaft of the second gear is fixedly connected to a fine adjustment lead screw. The first and second gears mesh. Both ends of the coarse adjustment lead screw are rotatably connected to the support-side lead screw support and the fixed-side lead screw support, respectively. Both ends of the fine adjustment lead screw are rotatably connected to the support-side lead screw support and the fixed-side lead screw support, respectively. The slide platform has protrusions on both sides of its bottom surface. These protrusions pass through two grooves on the surface of the cover plate. One protrusion is fixedly connected to a lead screw ball nut, which is threaded onto the coarse and fine adjustment lead screws. The other protrusion is fixedly connected to a bushing, which is fitted onto the guide shaft and slides axially along the guide shaft. The slide platform then slides back and forth on the guide shaft via the bushing.

[0006] Furthermore, the elastic clamping assembly includes a slide clamping block, a spring, and a guide pin. The slide clamping block is elastically disposed in a mounting groove at one end of the slide platform. The guide pin passes through a through hole on the slide platform, the spring, and a through hole at the lower end of the slide clamping block, and is fixedly connected to the slide platform. The spring is a compression spring, and its two ends are limited by the inner wall of the groove on the slide platform and the side wall of the slide clamping block. The slide clamping block moves linearly relative to the guide pin, and its maximum movement is limited by another groove on the slide platform. The slide is placed in the groove of the slide platform and clamped by the slide clamping block under the action of the spring.

[0007] Furthermore, a coarse adjustment screw is provided with a coarse adjustment screw wrench groove at one end near the support side screw support, and a fine adjustment screw is provided with a fine adjustment screw wrench groove at one end near the support side screw support, for adjusting the rotation of the coarse and fine adjustment screws; the coarse adjustment screw wrench groove and the fine adjustment screw wrench groove are exposed to the outside of the housing assembly through two through holes on the left side plate.

[0008] Furthermore, the objective lens assembly includes an objective lens, an objective lens mounting plate, a lifting and shifting stage, and a lifting and shifting stage mounting base; the lifting and shifting stage mounting base is fixed on the base of the housing assembly, the lifting and shifting stage is fixed on the lifting and shifting stage mounting base, the objective lens mounting plate is fixed on the lifting and shifting stage, and the objective lens is fixed on the objective lens mounting plate.

[0009] Furthermore, the lifting and displacement stage achieves the raising or lowering of the objective lens mounting plate on the lifting and displacement stage through the focusing assembly; the focusing assembly includes: a focusing screw mounting seat and a focusing screw; the focusing screw and the focusing screw mounting seat are connected by threads, and the ball head of the focusing screw mounting seat contacts the lifting mechanism inside the lifting and displacement stage.

[0010] Furthermore, the dichroic mirror assembly includes: a dichroic mirror mounting base, a second lens, a dichroic mirror insert, and a multi-channel dichroic mirror; the second lens is fixed in a hole on the end face of the dichroic mirror mounting base, the multi-channel dichroic mirror is fixed in a groove in the dichroic mirror insert, and the dichroic mirror is inserted into the dichroic mirror mounting base at a certain angle.

[0011] Furthermore, the lens hood assembly includes: a lens hood and an emitter plate; the lens hood is installed on the camera by a threaded connection, and the inside of the lens hood has an anti-light thread to prevent light leakage; the emitter plate is fixed on top; the position of the lens hood is adjusted by adjusting the length of the screw thread, ensuring that the front end face is always in contact with the end face of the lens.

[0012] Furthermore, the tube lens is mounted on the base of the housing assembly through a waist-shaped hole structure, enabling the tube lens to translate along the optical path. The fluorescence focusing position is adjusted by adjusting the position of the tube lens until the focal plane of the camera is clearly imaged.

[0013] Furthermore, ventilation holes are provided on the side panel of the enclosure assembly, and a fan is installed on the internal side panel of the enclosure assembly for internal heat dissipation.

[0014] The significant advantages of this invention compared to existing technologies are:

[0015] (1) The internal space of the fluorescence microscope device designed in this invention is fully utilized, the shape is regular, and the dimensions of the entire device are no more than 10cm×10cm×10cm;

[0016] (2) The present invention designs a slide moving assembly, which allows the sample to be placed in the entire area along the axial direction of the slide, and the slide moving range can ensure that the objective lens observation range in the device covers the entire slide.

[0017] (3) The present invention is equipped with two sets of adjusting screws, which can manually adjust the position of the glass slide at two speeds, fast and slow. The adjustment mechanism has a simple structure, allowing the operator to choose the adjustment speed and precision according to actual needs.

[0018] (4) The present invention designs a multi-color LED light source component with multi-color fluorescence channels and switching function between the multi-color fluorescence channels, thereby enabling simultaneous imaging of multiple targets and reducing illumination time, photobleaching and phototoxicity, which is particularly suitable for long-term observation of live cells.

[0019] (5) The fluorescence microscope designed in this invention is more conducive to modular integration and can be connected to external driving components as needed, which facilitates the automation transformation of fluorescence microscope. Attached Figure Description

[0020] Figure 1 This is a general appearance drawing of the device;

[0021] Figure 2 Schematic diagram of the external structure of the device Figure 1 ;

[0022] Figure 3 Schematic diagram of the external structure of the device Figure 2 ;

[0023] Figure 4 This is a schematic diagram of the internal structure of the device;

[0024] Figure 5 for Figure 4 Cross-sectional view of the internal structure of the device from direction A (optical path schematic diagram);

[0025] Figure 6 Schematic diagram of the glass slide moving assembly Figure 1 ;

[0026] Figure 7 for Figure 6 Sectional view of the slide moving assembly along direction B;

[0027] Figure 8 Schematic diagram of the glass slide moving assembly Figure 2 ;

[0028] Figure 9 This is a schematic diagram of the focusing assembly;

[0029] Figure 10 This is a schematic diagram of a multi-color LED light source assembly;

[0030] Figure 11 This is a cross-sectional view of a multi-color LED light source assembly along line C.

[0031] Figure 12 This is a schematic diagram of a dichroic mirror assembly;

[0032] Figure 13 A schematic diagram of a dichroic mirror assembly (with the dichroic mirror mounting bracket removed).

[0033] Figure 14 This is a schematic diagram of the sunshade assembly.

[0034] Figure label:

[0035] 1. Slide moving assembly; 2. Front side plate; 3. Base; 4. Left side plate; 111-1. Coarse adjustment screw wrench slot; 113-1. Fine adjustment screw wrench slot; 5. Right side plate; 6. Focusing assembly; 7. Wiring harness socket; 8. Rear side plate; 9. Camera; 10. Multicolor LED light source assembly; 11. Fan; 12. Support column; 13. Light shield assembly; 14. Camera mounting plate; 15. Tube lens; 16. Objective lens; 17. Objective lens mounting plate; 18. Lifting stage; 19. Dichroic mirror assembly; 20. Lifting stage mounting base; 21. Reflecting prism; 22. Prism mounting base; 23. Illumination light path; 24. Fluorescent light path; 101. Cover plate; 102. Slide platform; 103. Slide clamping block; 104. Slide; 105. Spring. 106. Guide pin; 107. Guide shaft support; 108. Support side lead screw support; 109. Large gear; 110. Small gear; 111. Coarse adjustment lead screw; 112. Lead screw ball nut; 113. Fine adjustment lead screw; 114. Locking nut; 115. Fixed side lead screw support; 116. Guide shaft; 117. Bushing; 111-1. Coarse adjustment lead screw wrench slot; 113-1. Fine adjustment lead screw wrench slot; 601. Focusing screw mounting seat; 602. Focusing screw; 1001. LED light source control board; 1002. Light guide rod support; 1003. Excitation plate mounting seat; 1004. Excitation plate; 1005. Lens one; 1006. LED light source; 1007. Light guide rod; 1901. Dichroic mirror mounting seat. 1902, Lens II; 1903, Dichroic Mirror Insertion Plate; 1904, Multi-channel Dichroic Mirror; 1301, Light Shield; 1302, Emitting Plate. Detailed Implementation

[0036] The terminology used in this invention is for illustrative purposes only and is not intended to limit the invention. The following description, in conjunction with the appendix, further clarifies this concept. Figure 1-14 The following is a detailed description of some embodiments of the present invention.

[0037] A small fluorescence microscopy imaging device includes: a box assembly, a slide moving assembly 1, an objective lens assembly, a reflecting prism assembly, a focusing assembly 6, a camera 9, a multicolor LED light source assembly 10, a fan 11, a dichroic mirror assembly 19, a light shield assembly 13, and a tube lens 15.

[0038] The enclosure assembly includes: a front panel 2, a base 3, a left side panel 4, a right side panel 5, and a rear panel 8; the side panels 2 and rear panel 8 are fixedly connected to the left side panel 4 and the right side panel 5, and the front panel 2, left side panel 4, right side panel 5, and rear panel 8 are all fixedly installed on the base 3. The slide moving assembly 1 is fixedly installed on the upper end of the front panel 2, left side panel 4, right side panel 5, and rear panel 8. The rear panel 8 has a through hole, and a wire harness socket 7 is installed at the corresponding position.

[0039] like Figure 4 As shown, inside the housing assembly, the components are installed as follows:

[0040] The multi-color LED light source assembly 10 is fixed on the rear panel 8 to provide light source; the fan 11 is fixed on the rear panel 8 by the support column 12 and the corresponding position of the rear panel 8 is provided with ventilation holes for heat dissipation.

[0041] The bottom surface of the camera 9 is in contact with the top surface of the base 3, and the two sides of the camera 9 are fixed on the camera mounting plate 14, which is fixed on the base 3.

[0042] The light shield assembly 13 is installed on the camera 9 by a threaded connection. The position of the light shield assembly 13 is adjusted by adjusting the length of the screw thread to ensure that the end face of the light shield assembly 13 is always in contact with the end face of the tube lens 15, thereby ensuring no light leakage. The tube lens 15 is fixed on the base 3. The tube lens 15 has waist-shaped grooves on both sides. The tube lens 15 can be moved along the X direction. The position of the fluorescence focusing position is adjusted by adjusting the position of the tube lens 15 until the focal plane of the camera 9 can be clearly imaged.

[0043] The reflecting prism assembly includes a reflecting prism 21 and a prism mounting base 22; the reflecting prism 21 is fixed on the prism mounting base 22, and the prism mounting base 22 is fixed on the base 3.

[0044] The objective lens assembly includes an objective lens 16, an objective lens mounting plate 17, a lifting stage 18, and a lifting stage mounting base 20. The lifting stage mounting base 20 is fixed on the base 3. The lifting stage 18 and the dichroic mirror assembly 19 are fixed on the lifting stage mounting base 20. The objective lens mounting plate 17 is fixed on the lifting stage 18, and the objective lens 16 is fixed on the objective lens mounting plate 17.

[0045] like Figure 6 , Figure 7 and Figure 8 As shown, the slide moving assembly 1 includes: a cover plate 101, a slide platform 102, an elastic clamping assembly, a slide clamping block 103, a slide 104, a spring 105, a guide pin 106, a guide shaft support 107, a support-side lead screw support 108, a large gear 109, a small gear 110, a coarse adjustment lead screw 111, a lead screw ball nut 112, a fine adjustment lead screw 113, a locking nut 114, a fixed-side lead screw support 115, a guide shaft 116, and a bushing 117.

[0046] The elastic clamping assembly includes a glass slide clamping block 103, a spring 105, and a guide pin 106;

[0047] like Figure 7As shown, the slide clamping block 103 is elastically disposed in the mounting groove at one end of the slide platform 102. Further, the guide pin 106 passes through a through hole on the slide platform 102, a spring 105, and a through hole at the lower end of the slide clamping block 103, and is fixedly connected to the slide platform 102. The spring 105 is a compression spring, and its two ends are limited by the inner wall of the groove of the slide platform 102 and the side wall of the slide clamping block 103. The slide clamping block 103 moves linearly relative to the guide pin 106, and its maximum movement position is limited by another groove in the slide platform 102. The slide 104 is placed in the groove of the slide platform 102 and clamped by the slide clamping block 103 under the action of the spring 105.

[0048] The guide shaft support 107, the support-side lead screw support 108, and the fixed-side lead screw support 115 are fixed on the cover plate 101. The central shaft of the large gear 109 is fixedly connected to the coarse adjustment lead screw 111, and the central shaft of the small gear 110 is fixedly connected to the fine adjustment lead screw 113. The large gear 109 and the small gear 110 mesh. The two ends of the coarse adjustment lead screw 111 are rotatably connected to the support-side lead screw support 108 and the fixed-side lead screw support 115 respectively through bearings. The two ends of the fine adjustment lead screw 113 are rotatably connected to the support-side lead screw support 108 and the fixed-side lead screw support 115 respectively through bearings. The locking nut 114 is installed at the ends of the coarse adjustment lead screw 111 and the fine adjustment lead screw 113 near the fixed-side lead screw support 115 for axial locking and limiting of the bearings. The coarse adjustment screw 111 has a coarse adjustment screw wrench groove 111-1 at one end near the support side screw support 108, and the fine adjustment screw 113 has a fine adjustment screw wrench groove 113-1 at one end near the support side screw support 108, for adjusting the rotation of the coarse adjustment screw 111 and the fine adjustment screw 113. The coarse adjustment screw wrench groove 111-1 and the fine adjustment screw wrench groove 113-1 are exposed outside the box assembly through two through holes on the left side plate 4; the bottom surface of the slide platform 102 has protrusions on both sides, and the two protrusions pass through two grooves on the surface of the cover plate 101. One side of the protrusion is fixedly connected to the screw ball nut 112, and the screw ball nut 112 is installed on the coarse adjustment screw 111 and the fine adjustment screw 113 by thread engagement; the other side of the protrusion is fixedly connected to the bushing 117, the bushing 117 is sleeved on the guide shaft 116 and can slide along the axial direction of the guide shaft 116, so that the slide platform 102 can slide back and forth on the guide shaft 116 through the bushing 117.

[0049] When a rapid coarse adjustment of the slide position is required, use a wrench to manually adjust the coarse adjustment screw 111-1 through the coarse adjustment screw wrench slot 111-1. This rotates the coarse adjustment screw 111, causing the screw ball nut 112 to move rapidly along the Y-axis, further moving the slide platform 102 rapidly along the Y-axis. When a slow, fine adjustment of the slide position is required, use a wrench to manually adjust the fine adjustment screw 113-1 through the fine adjustment screw wrench slot 113-1. This rotates the fine adjustment screw 113, causing the screw ball nut 112 to move slowly along the Y-axis, further moving the slide platform 102 slowly along the Y-axis.

[0050] The large gear 109 and the small gear 110 ensure that the speed ratio between the coarse adjustment screw 111 and the fine adjustment screw 113 remains constant. When either the coarse adjustment screw 111 or the fine adjustment screw 113 rotates, it will drive the other to rotate synchronously through the large gear 109 and the small gear 110, preventing the coarse adjustment screw 111 and the fine adjustment screw 113 from getting stuck during operation.

[0051] like Figure 9 As shown: Focusing assembly 6 includes: a focusing screw mounting base 601, a focusing screw 602, and...

[0052] The focusing screw 602 is connected to the focusing screw mounting base 601 by threads. The ball head of the focusing screw mounting base 601 contacts the end face of the wedge mechanism (i.e., the lifting mechanism) inside the lifting stage 18, which plays a driving role. By adjusting the length of the thread screw 602 screwed into the focusing screw mounting base 601, the upward or downward movement of the objective lens mounting plate 17 fixed on the lifting stage 18 is controlled, thereby driving 16 to rise or fall.

[0053] like Figure 1 and Figure 11 As shown, the multi-color LED light source assembly 10 includes: an LED light source control board 1001, a light guide rod support 1002, an excitation plate mounting base 1003, an excitation plate 1004, a lens 1005, an LED light source 1006, and a light guide rod 1007.

[0054] The LED light source control board 1001 and the light guide rod support 1002 are fixed on the rear side plate 8. The excitation plate mounting base 1003 is fixed on the light guide rod support 1002, and the excitation plate 1004 is fixed in the stepped hole of the excitation plate mounting base 1003. The lens 1005, the LED light source 1006, and the light guide rod 1007 are fixed in the hole of the excitation plate mounting base 1003.

[0055] The LED light source control board 1001 is used to control the switching of the LED light source 1006 and to switch between multi-color fluorescent channels.

[0056] like Figure 12 and Figure 13As shown, the dichroic mirror assembly 19 includes: a dichroic mirror mounting base 1901, a second lens 1902, a dichroic mirror insert 1903, and a multi-channel dichroic mirror 1904.

[0057] Dichroic mirror mounting base 1901 is fixed on lifting displacement platform mounting base 20, lens 1902 is fixed in the hole on the end face of dichroic mirror mounting base 1901, and multi-channel dichroic mirror 1904 is fixed in the groove of dichroic mirror plug 1903. Dichroic mirror plug 1903 is inserted into dichroic mirror mounting base 1901 at a certain angle for easy replacement and assembly.

[0058] like Figure 14 As shown, the light shield assembly 13 includes: a light shield 1301 and an emitting sheet 1302;

[0059] The light shield 1301 is installed on the camera 9 by means of a threaded connection. The inside of the light shield 1301 is provided with an anti-light thread to prevent light leakage. The emitting plate 1302 is fixed on the 1301. The position of the light shield 1301 is adjusted by adjusting the length of the thread screwed into the light shield 1301, ensuring that the front end face of the 1301 is always in contact with the end face of the tube lens 15, thereby ensuring no light leakage.

[0060] The working principle of the small fluorescence microscopy imaging device disclosed in this invention is as follows: Figure 5 As shown, the light emitted by the LED light source 1006 is filtered and collimated by lens 1005, excitation plate 1004, and lens 1902, and then reflected upward by multi-channel dichroic mirror 1904. It then shines on the sample area on the glass slide 104 through objective lens 16. The sample that is irradiated will emit fluorescence. The fluorescence propagates downward through objective lens 16 and then continues to pass through multi-channel dichroic mirror 1904 until it is reflected by reflection prism 21. Finally, it is focused and filtered by tube lens 15 and light shield assembly 13, and reaches the focal plane of camera 9 to complete fluorescence imaging. Figure 5 In the diagram, illumination path 23 is a schematic diagram of illumination path, and fluorescence path 24 is a schematic diagram of fluorescence path.

[0061] The method of using a small fluorescence microscopy imaging device disclosed in this invention is as follows:

[0062] (1) Preparation: First, adjust the position of the tube lens 15 to ensure that the light can be focused on the focal plane of the camera 9. Then, adjust the position of the lens hood assembly 13 to ensure that the end face of the lens hood assembly 13 is close to the end face of the tube lens 15 to avoid light leakage.

[0063] (2) After completing the preparation work, turn on the power, place the slide 104 into the groove of the slide platform 102 and press it with the slide platform 103, manually adjust the focusing screw mounting seat 601, thereby adjusting the objective lens 16 to move up and down along the Z axis until the sample can be clearly imaged by the camera.

[0064] (3) Then turn off the indoor lights and select to turn on different channels of LED light source (different channels can be superimposed) according to actual needs to complete the fluorescence imaging of the sample. When the sample area to be observed is far from the objective lens 16, the slide 104 can be quickly brought closer to the objective lens 16 along the Y-axis by turning the coarse adjustment screw 111 with a wrench. When it is closer, the slide 104 can be slowly brought closer to the objective lens 16 along the Y-axis by turning the fine adjustment screw 113 with a wrench until the ideal position is reached. The fast and slow modes can be switched back and forth according to actual needs, which is simple and convenient to operate. Since the stroke of the coarse adjustment screw 111 and the fine adjustment screw 113 is large enough, they can cover the sample area distributed along the Y-axis on the entire slide 104.

[0065] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention without departing from the principles and spirit of the present invention.

Claims

1. A small fluorescence microscopy imaging device, characterized in that, It includes a box assembly, a slide moving assembly (1), an objective lens assembly, a dichroic mirror assembly (19), a reflecting prism (21), a light shield assembly (13), a tube lens (15), a camera (9), and a multicolor LED light source assembly (10); the slide moving assembly (1) is installed on the upper part of the box assembly and is used to place the sample and move the sample. The objective lens assembly is installed below the slide moving assembly (1). The dichroic mirror assembly (19) is located below the objective lens (16) in the objective lens assembly. It is used to selectively reflect or transmit light of a specific wavelength to achieve optical path control. The reflecting prism (21) is located below the dichroic mirror assembly (19). The multicolor LED light source assembly (10) is located on the side of the dichroic mirror assembly (19) and is fixedly connected to the side plate of the housing assembly. The camera (9), the light shield assembly (13), and the tube lens (15) are arranged in sequence on the bottom plate of the housing assembly, and the optical path is parallel to the optical path of the LED light source assembly (10). The distance between the camera (9) and the light shield assembly (13) is continuously adjusted by relative rotation. The end face of the light shield assembly (13) is always in contact with the end face of the tube lens (15).

2. The miniature fluorescence microscopy imaging device according to claim 1, characterized in that, The slide moving assembly (1) includes: a cover plate (101), a slide platform (102), an elastic clamping assembly, a slide (104), a guide shaft support (107), a support side screw support (108), a first gear, a second gear, a coarse adjustment screw (111), a screw ball nut (112), a fine adjustment screw (113), a fixed side screw support (115), a guide shaft (116), and a bushing (117). The cover plate (101) is fixedly connected to the housing assembly. The slide (104) is placed on the slide platform (102) and pressed by the elastic clamping assembly. The guide shaft support (107), the support-side screw rod support (108), and the fixed-side screw rod support (115) are fixed on the cover plate (101). The central shaft of the first gear is fixedly connected to the coarse adjustment screw rod (111), and the central shaft of the second gear is fixedly connected to the fine adjustment screw rod (113). The first gear and the second gear mesh. The two ends of the coarse adjustment screw rod (111) are rotatably connected to the support-side screw rod support (108) and the fixed-side screw rod support (115) respectively. The two ends of the fine adjustment screw rod (113) are rotatably connected to the support-side screw rod support (108) and the fixed-side screw rod support (115) respectively. The supporting screw support (108) and the fixed screw support (115) are rotatably connected; the bottom surface of the slide platform (102) is provided with protrusions on both sides, and the two protrusions pass through two grooves on the surface of the cover plate (101). One side of the protrusion is fixedly connected to the screw ball nut (112), and the screw ball nut (112) is installed on the coarse adjustment screw (111) and the fine adjustment screw (113) by thread engagement; the other side of the protrusion is fixedly connected to the bushing (117), the bushing (117) is sleeved on the guide shaft (116) and slides along the axial direction of the guide shaft (116), so that the slide platform (102) slides back and forth on the guide shaft (116) through the bushing (117).

3. The miniature fluorescence microscopy imaging device according to claim 2, characterized in that, The elastic clamping assembly includes a slide clamping block (103), a spring (105), and a guide pin (106). The slide clamping block (103) is elastically disposed in the mounting groove at one end of the slide platform (102). The guide pin (106) passes through the through hole on the slide platform (102), the spring (105), the through hole at the lower end of the slide clamping block (103), and is fixedly connected to the slide platform (102). The spring (105) is a compression spring and its two ends are limited by the inner wall of the groove of the slide platform (102) and the side wall of the slide clamping block (103). The slide clamping block (103) moves linearly relative to the guide pin (106), and its maximum position of movement is limited by the other groove of the slide platform (102). The slide (104) is placed in the groove of the slide platform (102) and pressed by the slide clamping block (103) under the action of the spring (105).

4. The miniature fluorescence microscopy imaging device according to claim 2, characterized in that, The coarse adjustment screw (111) has a coarse adjustment screw wrench groove (111-1) at one end near the support side screw support (108), and the fine adjustment screw (113) has a fine adjustment screw wrench groove (113-1) at one end near the support side screw support (108), which are used to adjust the rotation of the coarse adjustment screw (111) and the fine adjustment screw (113); the coarse adjustment screw wrench groove (111-1) and the fine adjustment screw wrench groove (113-1) are exposed to the outside of the housing assembly through two through holes on the left side plate (4).

5. The miniature fluorescence microscopy imaging device according to claim 2, characterized in that, The objective lens assembly includes an objective lens (16), an objective lens mounting plate (17), a lifting stage (18), and a lifting stage mounting base (20); the lifting stage mounting base (20) is fixed on the base of the housing assembly, the lifting stage (18) is fixed on the lifting stage mounting base (20), the objective lens mounting plate (17) is fixed on the lifting stage (18), and the objective lens (16) is fixed on the objective lens mounting plate (17).

6. The miniature fluorescence microscopy imaging device according to claim 5, characterized in that, The lifting stage (18) raises or lowers the objective lens mounting plate (17) on the lifting stage (18) through the focusing assembly (6); the focusing assembly (6) includes: a focusing screw mounting seat (601) and a focusing screw (602); the focusing screw (602) and the focusing screw mounting seat (601) are connected by threads, and the ball head of the focusing screw mounting seat (601) contacts the lifting mechanism inside the lifting stage (18).

7. The miniature fluorescence microscopy imaging device according to claim 1, characterized in that, The dichroic mirror assembly (19) includes: a dichroic mirror mounting base (1901), a second lens (1902), a dichroic mirror insert (1903), and a multi-channel dichroic mirror (1904); the second lens (1902) is fixed in the hole on the end face of the dichroic mirror mounting base (1901), the multi-channel dichroic mirror (1904) is fixed in the groove of the dichroic mirror insert (1903), and the dichroic mirror insert (1903) is inserted into the dichroic mirror mounting base (1901) at a certain angle.

8. The miniature fluorescence microscopy imaging device according to claim 1, characterized in that, The light shield assembly (13) includes: a light shield (1301) and an emitter (1302); the light shield (1301) is installed on the camera (9) by means of a threaded connection, and the inside of the light shield (1301) is provided with an anti-light thread to prevent light leakage; the emitter (1302) is fixed on (1301); by adjusting the length of the thread screwed into the light shield (1301), the position of the light shield (1301) is adjusted to ensure that the front end face of (1301) is always in contact with the end face of the tube lens (15).

9. The miniature fluorescence microscopy imaging device according to claim 1, characterized in that, The tube (15) is mounted on the base of the box assembly through a waist-shaped hole structure, so that the tube (15) can be translated along the optical path. The position of the fluorescence focusing position is adjusted by adjusting the position of the tube (15) until the focal plane of the camera (9) is clearly imaged.

10. The miniature fluorescence microscopy imaging device according to claim 1, characterized in that, Ventilation holes are provided on the side panel of the enclosure assembly, and a fan is installed on the internal side panel of the enclosure assembly for internal heat dissipation.

Images