Ginseng oligopeptide-containing polypeptide composite solution firming and anti-wrinkle composition and application thereof

Abstract

The invention relates to the technical field of skin care products, in particular to a ginseng oligopeptide-containing polypeptide composite solution firming and anti-wrinkle composition which comprises ginseng oligopeptide and a polypeptide composite solution, and the polypeptide composite solution comprises acetyl hexapeptide-8, snake venom-like tripeptide, hexapeptide-9 and acetyl dipeptide-1. Through the cooperation of the polypeptide and the ginseng oligopeptide, collagen generation is enhanced, skin aging inducements are reduced by means of the anti-aging, anti-oxidation and anti-inflammatory characteristics of the ginseng oligopeptide, and more comprehensive anti-wrinkle and firming effects are achieved.

Description

Technical Field

[0001] This invention relates to the field of skincare technology, specifically to a peptide complex solution containing ginseng oligopeptides for firming and anti-wrinkle effects, and its application. Background Technology

[0002] The formation of wrinkles and loss of elasticity caused by skin aging are the core focus of skincare. One of the key causes is the overactivity of elastase synthesized and secreted by fibroblasts in the skin. This enzyme is a highly selective and specific proteolytic enzyme that catalyzes the hydrolysis of carboxyl-containing polypeptide bonds in many amino acids, such as glycine, leucine, alanine, and valine. It can decompose elastin in connective tissue proteins, leading to damage to the skin's supporting structure and thus wrinkles and sagging.

[0003] Existing technologies combine peptides with conventional moisturizing / antioxidant ingredients, focusing on the collagen synthesis-stimulating effect of peptides alone, as well as the moisturizing and anti-wrinkle effects of auxiliary ingredients; however, they are insufficient in addressing the multiple factors of skin aging, and their anti-wrinkle and firming effects are limited in terms of duration and comprehensiveness.

[0004] In addition, there are many anti-wrinkle and firming products on the market that use ginseng extract. Ginseng-related extracts are widely used because they promote collagen production. However, ginseng extracts are made into finished products through a simple physical extraction process. Their active ingredients are large molecular proteins, which are not easily absorbed by human skin and have a low transdermal absorption rate. Summary of the Invention

[0005] In view of the above-mentioned shortcomings of the prior art, the present invention provides a peptide complex solution containing ginseng oligopeptides for firming and anti-wrinkle effects and its application, which can effectively solve the above-mentioned problems of the prior art. Technical solution

[0006] To achieve the above objectives, the present invention provides the following technical solution: This invention provides a firming and anti-wrinkle composition containing ginseng oligopeptide and a polypeptide complex solution, which includes ginseng oligopeptide and a polypeptide complex solution, wherein the polypeptide complex solution includes acetyl hexapeptide-8, snake venom-like tripeptide, hexapeptide-9 and acetyl dipeptide-1.

[0007] Furthermore, the polypeptide complex solution comprises acetyl hexapeptide-8, snake venom-like tripeptide, hexapeptide-9, and acetyl dipeptide-1, with a weight ratio of 0.5-1.5:4-6:1-3:8-12.

[0008] Furthermore, the polypeptide complex solution comprises acetyl hexapeptide-8, snake venom-like tripeptide, hexapeptide-9, and acetyl dipeptide-1, in a weight ratio of 1:5:2:10.

[0009] Furthermore, the volume ratio of the ginseng oligopeptide to the polypeptide complex solution is 1:(1-10).

[0010] Furthermore, the volume ratio of the ginseng oligopeptide to the polypeptide complex solution is 1:(1-3).

[0011] Preferably, the volume ratio of the ginseng oligopeptide to the polypeptide complex solution is 1:2.

[0012] Furthermore, the ginseng oligopeptides are obtained through a process of ginseng → extraction → separation → hydrolysis → filtration → separation → purification → testing.

[0013] Preferably, the specific steps for preparing the ginseng oligopeptide are as follows: S1: Take a whole ginseng plant, wash it with pure water and drain it. Then slice it and bake it in an oven at 50-60℃ for 3-5 hours. Dry it and then pulverize it. Then take the ginseng powder and soak it in water at a ratio of 50-150g ginseng to 1.2L water to extract the supernatant. S2: Take the supernatant and add papain and pectinase in a weight ratio of 1:9-9:1, with 3:1 being the optimal ratio. Hydrolyze ginseng protein at 40-60℃ for 6-8 hours to obtain ginseng oligopeptides with smaller molecular weight. S3: Ginseng oligopeptides are obtained by centrifuging and adsorption resin column to obtain purer ginseng oligopeptides, and then dried by freeze dryer to obtain ginseng oligopeptide powder; S4: Ginseng oligopeptide powder is dissolved in 1,2-hexanediol, 1,2-pentanediol, and water to prepare a ginseng oligopeptide solution. The solution comprises, by weight percentage: 20%-30% ginseng oligopeptide, 1-3% 1,2-hexanediol, 0.5-2% 1,2-pentanediol, and 65-78.5% water.

[0014] More preferably, the specific steps for preparing the ginseng oligopeptide are as follows: S1: Take a whole ginseng plant, wash it with pure water and drain it. Then slice it, put it in a 58℃ oven and bake it for 4 hours to dry it and pulverize it. Then take the ginseng powder and soak it in water at a ratio of 100g ginseng to 1.2L water to extract the supernatant. S2: Take the supernatant and add papain and pectinase in a weight ratio of 1:9-9:1, with 3:1 being the optimal ratio. Hydrolyze ginseng protein at 50℃ for 7 hours to obtain ginseng oligopeptides with smaller molecular weight. S3: Ginseng oligopeptides are obtained by centrifuging and adsorption resin column to obtain purer ginseng oligopeptides, and then dried by freeze dryer to obtain ginseng oligopeptide powder; S4: Ginseng oligopeptide powder was dissolved in 1,2-hexanediol, 1,2-pentanediol, and water to prepare a ginseng oligopeptide solution. The solution, by weight percentage, consisted of 25% ginseng oligopeptide, 2% 1,2-hexanediol, 1% 1% 1%, and 70% water.

[0015] Furthermore, in the hydrolysis step, ginseng oligopeptides are obtained by adding protease and centrifuging under an adsorption resin column.

[0016] Furthermore, the protease includes papain and pectinase, and the weight ratio of papain to pectinase is 1:9-9:1; preferably, the weight ratio of papain to pectinase is 3:1.

[0017] A peptide complex solution containing ginseng oligopeptides is used for skin firming and anti-wrinkle purposes.

[0018] A peptide complex solution containing ginseng oligopeptides is used in cosmetics to create a firming and anti-wrinkle composition. Beneficial effects

[0019] The technical solution provided by this invention has the following advantages compared with known public technologies: (1) This invention utilizes extracts (containing ginseng oligopeptides) from the whole ginseng plant (including roots, stems and leaves) in combination with polypeptides (acetyl hexapeptide-8, snake venom-like tripeptide, hexapeptide-9, acetyl dipeptide-1); by weight percentage, the combination ratio of 10% ginseng oligopeptide, 0.01% acetyl hexapeptide-8, 0.05% snake venom-like tripeptide, 0.02% hexapeptide-9, and 0.1% acetyl dipeptide-1 results in a polypeptide complex solution that provides excellent anti-aging and firming effects; (2) This invention studies multiple aspects. First, through a special enzymatic hydrolysis technique, ginseng oligopeptides with an average molecular weight of 1000 Da or less are obtained. Among them, 80% of the ginseng oligopeptides have a molecular weight of less than 1000 Da, and 58% of the ginseng oligopeptides have a molecular weight of less than 500 Da. The total number of polypeptides in the ginseng oligopeptides is 1085, which can penetrate the skin and enter the basal layer to achieve the highly effective anti-wrinkle and firming effect of ginseng extract. Second, the ginseng oligopeptides are compounded with acetyl hexapeptide-8, snake venom-like tripeptide, hexapeptide-9, and acetyl dipeptide-1. The excellent formulation ratio of the compound solution effectively improves its anti-wrinkle and firming effect, and enhances the stability and skin compatibility of the ingredients.

[0020] (3) The present invention is a specific combination of "polypeptide complex solution containing ginseng oligopeptide". The polypeptide complex solution contains a mixture of multiple peptides such as ginseng oligopeptide, acetyl hexapeptide-8, snake venom tripeptide, hexapeptide-9, and acetyl dipeptide-1, focusing on the anti-wrinkle and firming effect of the polypeptide synergistic effect.

[0021] (4) This invention enhances collagen production through the synergistic effect of polypeptides and ginseng oligopeptides, and reduces the causes of skin aging by taking advantage of the anti-aging, antioxidant and anti-inflammatory properties of ginseng oligopeptides, thus achieving a more comprehensive anti-wrinkle and firming effect.

[0022] (5) The present invention obtains ginseng oligopeptides through a special enzymatic hydrolysis process. Ginseng oligopeptides are polypeptide complexes obtained by converting proteins extracted from ginseng through an exclusive biological enzyme. The average molecular weight of the peptide segments is less than 1000 Daltons, which is conducive to transdermal absorption. The total number of polypeptides in ginseng oligopeptides is 1085. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below.

[0024] Figure 1 This is a flowchart of the extraction process for ginseng oligopeptides. Figure 2 The test results for different concentrations of polypeptide complex solutions are shown below. (When performing statistical analysis using software, p < 0.05 indicates a statistically significant difference between the positive control group and the test sample group and the negative control group, indicated by "*"; p ≥ 0.05 indicates no statistically significant difference). Figure 3 The results of testing different concentrations of ginseng oligopeptides are shown below. (When performing statistical analysis using software, p < 0.05 indicates a statistically significant difference between the positive control group and the test sample group and the negative control group, indicated by "*"; p ≥ 0.05 indicates no statistically significant difference). Figure 4 The test results for different concentrations of ginseng oligopeptide + polypeptide complex solution are shown below. (When using statistical analysis software for analysis, the positive control group and the test sample group are compared with the negative control group. p < 0.05 indicates statistical difference, marked with "*"; p ≥ 0.05 indicates no statistical difference). Detailed Implementation

[0025] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0026] The present invention will be further described below with reference to embodiments.

[0027] (1) The extraction process of ginseng oligopeptides is as follows: Figure 1 As shown, it specifically includes: S1: Take a whole ginseng plant, wash it with pure water and drain it. Then slice it, put it in a 58℃ oven and bake it for 4 hours to dry it and then pulverize it. Then take the ginseng powder and soak it in water at a ratio of 100g ginseng to 1.2L water to extract the supernatant.

[0028] S2: Take the supernatant and add papain and pectinase in a 3:1 ratio, and hydrolyze ginseng protein at 50℃ for 7 hours to obtain ginseng oligopeptides with smaller molecular weight.

[0029] S3: Ginseng oligopeptides are pretreated with macroporous adsorption resin (XAD-16N) by centrifugation to obtain purer ginseng oligopeptides, which are then dried by freeze dryer to obtain ginseng oligopeptide powder.

[0030] S4: Ginseng oligopeptide powder was dissolved in 1,2-hexanediol, 1,2-pentanediol, and water to prepare a ginseng oligopeptide solution. The solution, by weight percentage, consisted of 25% ginseng oligopeptide, 2% 1,2-hexanediol, 1% 1%, and the balance being water.

[0031] (2) Polypeptide complex solution: composed of acetyl hexapeptide-8, snake venom tripeptide, hexapeptide-9, and acetyl dipeptide-1, with a weight ratio of 1:5:2:10.

[0032] (1) Principle of testing Elastase is mainly synthesized and secreted by fibroblasts. It is a protein-degrading enzyme with extremely high selectivity and specificity. It catalyzes the hydrolysis of many amino acids, such as glycine, leucine, alanine, and valine, which contain carboxyl groups in their polypeptide bonds. This can cause the breakdown of elastin in connective tissue proteins, leading to skin laxity and wrinkles.

[0033] The principle of the elastase inhibition assay is that elastase reacts with the enzyme substrate, and the absorbance changes after the addition of the active substance. The magnitude of the change in absorbance can reflect the inhibition rate of the elastase inhibitor.

[0034] This test method is an in vitro method, which mainly characterizes the anti-wrinkle and firming efficacy by evaluating the inhibition rate of elastase activity of the test sample. It is suitable for evaluating cosmetics that achieve anti-wrinkle and firming effects by inhibiting elastase.

[0035] (2) Test method Add appropriate amounts of buffer solution, sample solutions or control solutions of different concentrations, and substrate to a 96-well plate. After shaking and mixing, incubate for 20 minutes. Then add elastase solution, shake and incubate for 10 minutes immediately. Measure the absorbance at 410 nm using a microplate reader. Analyze the data and calculate the inhibition rate of the test sample against elastase.

[0036] Result calculation: Elastase inhibition rate (%) = [1 - (AB) / (CD)] × 100% In the formula: A represents the absorbance of the experimental group (containing substrate, buffer solution, elastase solution, and sample solution (sample group) / quercetin solution (positive control group) / water (negative control group)). B represents the absorbance of the experimental blank group (containing substrate, containing buffer solution, without elastase solution, containing sample solution (sample group) / quercetin solution (positive control group) / water (negative control group)). C represents the absorbance of the control group (containing substrate, buffer solution, elastase solution, and no sample solution). D represents the absorbance of the blank group (containing substrate, buffer solution, elastase solution, and sample solution).

[0037] (3) Test results The polypeptide complex solution prepared in sample preparation (I) above was used. The polypeptide complex solution consisted of acetyl hexapeptide-8, snake venom-like tripeptide, hexapeptide-9, and acetyl dipeptide-1 in a volume ratio of 1:5:2:10. Sample groups of different concentrations were prepared by diluting the above polypeptide complex solution with water, and their elastase inhibition rates, dilution ratios, and test results are shown in Tables 1 and 2. Figure 2 As shown.

[0038] The positive control group consisted of quercetin solution, while the negative control group consisted of water.

[0039] Table 1. Test results of polypeptide complex solutions of different concentrations Note: p < 0.05 indicates a statistically significant difference compared to the negative control group; p ≥ 0.05 indicates no statistically significant difference compared to the negative control group.

[0040] When the concentration of the positive control group was 200 µg / mL, the elastase inhibition rate was ≥90%, indicating that the reaction system was effective; When the concentration of the test sample group (0.1%) was 0.1% (v / v), the elastase inhibition rate was approximately 22.59%; when the concentration of the test sample group (0.2%) was 0.2% (v / v), the elastase inhibition rate was approximately 16.94%; when the concentration of the test sample group (0.5%) was 0.5% (v / v), the elastase inhibition rate was approximately 14.62%; and when the concentration of the test sample group (1.0%) was 1.0% (v / v), the elastase inhibition rate was approximately 10.96%. These results showed statistically significant differences compared to the negative control group (p < 0.05), indicating that the polypeptide complex solution has the ability to inhibit elastase activity at the tested concentrations. This can serve as one of the pieces of evidence supporting the claim that the sample has anti-wrinkle and firming effects.

[0041] The ginseng oligopeptides prepared in sample preparation (I) above were then compounded with water to prepare sample groups of different concentrations. The elastase inhibition rate, dilution ratio, and test results are shown in Table 2 and 3. Figure 3 As shown in the figure. The positive control group consisted of quercetin solution, and the negative control group consisted of water.

[0042] Table 2. Test results of ginseng oligopeptides at different concentrations Note: p < 0.05 indicates a statistically significant difference compared to the negative control group; p ≥ 0.05 indicates no statistically significant difference compared to the negative control group.

[0043] When the concentration of the positive control group was 200 µg / mL, the elastase inhibition rate was ≥90%, indicating that the reaction system was effective; When the concentration of the test sample group (0.1%) was 0.1% (v / v), the elastase inhibition rate was approximately 27.57%; when the concentration of the test sample group (0.2%) was 0.2% (v / v), the elastase inhibition rate was approximately 22.92%; when the concentration of the test sample group (0.5%) was 0.5% (v / v), the elastase inhibition rate was approximately 18.94%; and when the concentration of the test sample group (1.0%) was 1.0% (v / v), the elastase inhibition rate was approximately 12.62%. These results showed statistically significant differences compared to the negative control group (p < 0.05), indicating that ginseng extract (ginseng oligopeptide) has the ability to inhibit elastase activity at the tested concentrations. This can serve as one of the pieces of evidence supporting the claim that the sample has anti-wrinkle and firming effects.

[0044] Sample groups 1 and 2 were prepared using the ginseng oligopeptide and polypeptide complex solution obtained in sample preparation (I) above, and were compounded according to the proportions in the table below. The positive control group consisted of quercetin solution, and the negative control group consisted of water.

[0045] Table 3. Test results of ginseng oligopeptide + polypeptide complex solutions of different concentrations Note: p < 0.05 indicates a statistically significant difference compared to the negative control group; p ≥ 0.05 indicates no statistically significant difference compared to the negative control group.

[0046] According to the test results: when the concentration of the positive control group was 200 µg / mL, the elastase inhibition rate was ≥90%, indicating that the reaction system was effective; when the concentration of the test sample group 1 was 0.1% ginseng extract (ginseng oligopeptide) + 0.2% polypeptide complex solution + 99.7% water, the elastase inhibition rate was approximately 10.57%; when the concentration of the test sample group 2 was 0.5% ginseng extract (ginseng oligopeptide) + 1% polypeptide complex solution + 98.5% water, the elastase inhibition rate was approximately 13.96%. Both showed statistically significant differences compared with the negative control group (p < 0.05), indicating that the ginseng extract (ginseng oligopeptide) + polypeptide complex solution had the ability to inhibit elastase activity at the tested concentrations, which can be used as one of the supporting evidences for the claim that the sample has anti-wrinkle and firming effects.

[0047] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the protection scope of the technical solutions of the embodiments of the present invention.

Claims

1. A peptide complex solution containing ginseng oligopeptides for firming and anti-wrinkle effects, characterized in that, It includes a ginseng oligopeptide and polypeptide complex solution, wherein the polypeptide complex solution includes acetyl hexapeptide-8, snake venom-like tripeptide, hexapeptide-9 and acetyl dipeptide-1.

2. The peptide complex solution containing ginseng oligopeptides for firming and anti-wrinkle effects according to claim 1, characterized in that, The polypeptide complex solution comprises acetyl hexapeptide-8, snake venom-like tripeptide, hexapeptide-9, and acetyl dipeptide-1, in a weight ratio of 0.5-1.5:4-6:1-3:8-12.

3. The peptide complex solution containing ginseng oligopeptides for firming and anti-wrinkle effects according to claim 1, characterized in that, The volume ratio of the ginseng oligopeptide to polypeptide complex solution is 1:(1-10).

4. The peptide complex solution containing ginseng oligopeptides for firming and anti-wrinkle effects according to claim 1, characterized in that, The volume ratio of the ginseng oligopeptide to polypeptide complex solution is 1:(1-3).

5. The peptide complex solution containing ginseng oligopeptides for firming and anti-wrinkle effects according to claim 1, characterized in that, The ginseng oligopeptides were obtained through a process of ginseng extraction, separation, hydrolysis, filtration, purification, and testing.

6. The peptide complex solution containing ginseng oligopeptides for firming and anti-wrinkle effects according to claim 1, characterized in that, The hydrolysis step involves adding a protease and obtaining ginseng oligopeptides in a centrifuge and adsorption resin column.

7. The peptide complex solution containing ginseng oligopeptides for firming and anti-wrinkle effects according to claim 1, characterized in that, The protease includes papain and pectinase, and the weight ratio of the papain to pectinase is 1:9-9:

1.

8. A peptide complex solution containing ginseng oligopeptide as described in any one of claims 1-7 for skin firming and anti-wrinkle purposes.

9. A firming and anti-wrinkle composition containing ginseng oligopeptide as described in any one of claims 1-7, used in cosmetics.

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