A cosmetic composition, method of making and use thereof

Abstract

The present application relates to the field of medical or cosmetic formulations, the field of biopharmaceutical manufacturing technology and the field of preparation manufacturing technology, and in particular to a cosmetic composition, a preparation method and application thereof.The cosmetic composition comprises an active ingredient; the active ingredient comprises a chloranthus specious extract and a rhizoma corydalis extract; and the weight ratio of the chloranthus specious extract and the rhizoma corydalis extract is 0.6:1-1:1.The chloranthus specious extract and the rhizoma corydalis extract in the cosmetic composition provided by the present application are synergistic with each other, and have excellent soothing and repairing effects.

Description

A cosmetic composition, its preparation method and application Technical Field

[0001] This invention relates to the fields of medical or cosmetic formulations, biopharmaceutical manufacturing technology, and pharmaceutical manufacturing technology, specifically to a cosmetic composition, its preparation method, and its application. Background Technology

[0002] In recent years, more and more people have been troubled by sensitive skin, a skin condition characterized by intolerance to changes in the external environment and the use of skincare products. There are three main mechanisms underlying sensitive skin: impaired skin barrier function, epidermal inflammation, and abnormalities in the skin's sensory nervous system. These mechanisms often interact, creating a vicious cycle that leads to sensitive skin. Sensitive skin is accompanied by subjective sensations such as tightness, stinging, short-term burning, and persistent itching, or objective phenomena such as telangiectasia, flushing, erythema, desquamation, and dryness. Furthermore, chronic inflammatory skin diseases such as atopic dermatitis and rosacea share some overlap in their pathogenesis with sensitive skin, but their symptoms are more persistent and have characteristic clinical manifestations. Atopic dermatitis is often accompanied by intense itching and dry, scaly skin, while rosacea is characterized by paroxysmal facial flushing, telangiectasia, and papules and pustules, severely impacting patients' quality of life. Patients with severe sensitive skin, atopic dermatitis, rosacea, diabetic foot, and other skin lesions during acute flare-ups are prone to symptoms such as redness, itching, papules, oozing, and burning due to external stimuli. They need a safe and effective combination that soothes and repairs skin discomfort, is waterproof, and provides local occlusion to address these issues. Summary of the Invention

[0003] To address the aforementioned technical problems, the present invention provides the following technical solutions.

[0004] In a first aspect, the present invention provides a cosmetic composition.

[0005] A cosmetic composition comprising: an active ingredient; said active ingredient including *Anoectochilus roxburghii* extract and *Corydalis yanhusuo* root extract; said *Anoectochilus roxburghii* extract is a water-based extract, and said *Corydalis yanhusuo* root extract is an ethanol-based extract; the weight ratio of said *Anoectochilus roxburghii* extract to said *Corydalis yanhusuo* root extract is 0.6:1 to 1:1. This invention, through the synergistic effect of *Anoectochilus roxburghii* extract and *Corydalis yanhusuo* root extract, can significantly reduce the hemoglobin content in the skin, effectively improve skin erythema, redness, and other inflammatory conditions, and can also significantly reduce transepidermal water loss, enhance skin barrier function, and is suitable for repairing damaged skin barrier, dryness, and sensitivity; it can also effectively inhibit hyaluronidase activity, helping to reduce the degradation of hyaluronic acid in the skin, thereby maintaining skin moisture and elasticity, and exerting anti-inflammatory and soothing effects.

[0006] In some embodiments, the weight ratio of the Anoectochilus roxburghii extract and the Corydalis yanhusuo root extract is 0.6:1, 0.7:1, 0.8:1, 0.9:1, 1:1, or any value within the range of any two of these values.

[0007] Corydalis yanhusuo, also known as Yuanhu, is the dried tuber of the plant Corydalis yanhusuo W.T.Wang, belonging to the Papaveraceae family. It is harvested in early summer when the stems and leaves wither. The fibrous roots are removed, and the tubers are washed and boiled or steamed in boiling water until just past the white core disappears. Then it is removed and sun-dried. The properties and channels of Corydalis yanhusuo are pungent, bitter, and warm. It enters the liver and spleen channels. The functions and indications of Corydalis yanhusuo are to invigorate blood circulation, regulate qi, and relieve pain. It is used for chest and rib pain, abdominal pain, angina pectoris, amenorrhea, dysmenorrhea, postpartum blood stasis, and traumatic swelling and pain.

[0008] *Anoectochilus roxburghii* (Wall.) Lindl., also known as Golden Thread Orchid, Golden Silk Grass, Bird Ginseng, Golden Thread Tiger Head Banana, and Golden Thread Bone-Eliminating Herb, is a perennial rare Chinese medicinal herb belonging to the genus *Anoectochilus* of the Orchidaceae family. It is mainly distributed in southern provinces of my country, including Fujian, Guangdong, and Zhejiang, with Fujian being its authentic producing area. The entire plant can be used medicinally. The *Fujian Provincial Standard for Traditional Chinese Medicine* (2006 edition) records that *Anoectochilus roxburghii* is a rare folk herb from Fujian and Taiwan provinces. Its properties are neutral and sweet; it clears heat and cools the blood, dispels wind and promotes diuresis; it is used for nephritis, bronchitis, cystitis, diabetes, rheumatoid arthritis, infantile convulsions, and snake bites. *Anoectochilus roxburghii* is rich in flavonoids, polysaccharides, glycosides, amino acids, triterpenoids, esters, sterols, and trace elements, possessing rich biological activity and effectively combating inflammation, scavenging free radicals, and anti-aging effects.

[0009] The *Anoectochilus roxburghii* used in this invention is an artificially cultivated *Anoectochilus roxburghii*.

[0010] In some embodiments, the preparation method of the Anoectochilus roxburghii extract includes: pulverizing the dried whole Anoectochilus roxburghii herb to obtain Anoectochilus roxburghii powder; then mixing the Anoectochilus roxburghii powder with water, centrifuging, collecting the supernatant, concentrating, and freeze-drying to obtain the Anoectochilus roxburghii extract.

[0011] In some embodiments, the preparation method of the Corydalis root extract includes: soaking the crude Corydalis root powder in ethanol and then refluxing it for extraction, evaporating and concentrating the extract until it becomes viscous, and then vacuum drying it to obtain the Corydalis root extract.

[0012] In some embodiments, the cosmetic composition may further include other active ingredients and cosmetically acceptable excipients.

[0013] In some embodiments, the other active ingredient includes tetrahydromethylpyrimidine carboxylic acid.

[0014] In some embodiments, the cosmetically acceptable excipients include at least one selected from humectants, thickeners, emulsifiers, osmotic pressure regulators, antioxidants, skin feel modifiers, penetration enhancers, pH adjusters, and solvents.

[0015] In some embodiments, the moisturizer comprises at least one selected from trehalose, beta-glucan, glycerin, and sodium hyaluronate.

[0016] In some embodiments, the thickener comprises: distearate dimethylammonium lithium montmorillonite.

[0017] In some embodiments, the emulsifier comprises at least one of: polydimethylsiloxane, polydimethylsiloxane PEG-10 / 15 crosspolymer, PEG-10 polydimethylsiloxane, and lauryl PEG-9 polydimethylsiloxane ethyl polydimethylsiloxane.

[0018] In some embodiments, the osmotic pressure regulator includes sodium chloride.

[0019] In some embodiments, the antioxidant includes p-hydroxyacetophenone.

[0020] In some embodiments, the skin feel modifier comprises at least one of 1,2-hexanediol, cyclopentamethoxysiloxane, cyclohexylsiloxane, and isononyl isononanoate. In some embodiments, the skin feel modifier comprises 1,2-hexanediol, cyclopentamethoxysiloxane, cyclohexylsiloxane, and isononyl isononanoate.

[0021] In some embodiments, the penetration enhancer includes caprylic / capric triglyceride.

[0022] In some embodiments, the pH adjuster includes citric acid.

[0023] In some embodiments, the solvent comprises 1,3-butanediol and water.

[0024] In some embodiments, the weight ratio of 1,3-butanediol to water in the solvent is 1:4 to 1:5. In some embodiments, the weight ratio of 1,3-butanediol to water in the solvent is 1:4, 1:4.1, 1:4.2, 1:4.3, 1:4.4, 1:4.5, 1:4.6, 1:4.7, 1:4.8, 1:4.9, 1:5, or any value within a range of any two of these values. In some embodiments, the weight ratio of 1,3-butanediol to water in the solvent is 1:4.5 to 1:4.7.

[0025] In some embodiments, based on the total weight of the cosmetic composition, the content of the active ingredient is 0.08 wt% to 0.10 wt%, the content of other active ingredients is 0.20 wt% to 0.50 wt%, the content of the moisturizer is 5.00 wt% to 6.00 wt%, the content of the thickener is 0.30 wt% to 0.50 wt%, the content of the emulsifier is 6.00 wt% to 6.50 wt%, the content of the osmotic pressure regulator is 0.70 wt% to 0.90 wt%, the content of the antioxidant is 0.40 wt% to 0.60 wt%, the content of the skin feel modifier is 9.00 wt% to 10.00 wt%, the content of the penetration enhancer is 3.00 wt% to 3.50 wt%, the content of the pH adjuster is 0.02 wt% to 0.04 wt%, and the content of the solvent is 70.00 wt% to 75.00 wt%.

[0026] In some embodiments, the content of the active ingredient, calculated based on the total weight of the cosmetic composition, is 0.08 wt%, 0.081 wt%, 0.082 wt%, 0.083 wt%, 0.084 wt%, 0.085 wt%, 0.086 wt%, 0.087 wt%, 0.088 wt%, 0.089 wt%, 0.090 wt%, 0.091 wt%, 0.092 wt%, 0.093 wt%, 0.094 wt%, 0.095 wt%, 0.096 wt%, 0.097 wt%, 0.098 wt%, 0.099 wt%, 0.10 wt%, or any value within a range of any two of these values.

[0027] In some embodiments, the content of the other active ingredients, calculated based on the total weight of the cosmetic composition, is 0.20 wt%, 0.25 wt%, 0.30 wt%, 0.35 wt%, 0.40 wt%, 0.45 wt%, 0.50 wt%, or any value within a range of any two of these values.

[0028] In some embodiments, the content of the moisturizer, calculated based on the total weight of the cosmetic composition, is 5.00 wt%, 5.10 wt%, 5.20 wt%, 5.30 wt%, 5.40 wt%, 5.50 wt%, 5.60 wt%, 5.70 wt%, 5.80 wt%, 5.90 wt%, 6.00 wt%, or any value within a range of any two of these values.

[0029] In some embodiments, the content of the thickener is calculated based on the total weight of the cosmetic composition as 0.30 wt%, 0.35 wt%, 0.40 wt%, 0.45 wt%, 0.50 wt%, or any value within a range of any two of these values.

[0030] In some embodiments, the emulsifier content is calculated based on the total weight of the cosmetic composition as 6.00 wt%, 6.10 wt%, 6.20 wt%, 6.30 wt%, 6.40 wt%, 6.50 wt%, or any value within a range of any two of these values.

[0031] In some embodiments, the content of the osmotic pressure regulator is calculated based on the total weight of the cosmetic composition as 0.70 wt%, 0.75 wt%, 0.80 wt%, 0.85 wt%, 0.90 wt%, or any value within a range of any two of these values.

[0032] In some embodiments, the antioxidant content is calculated based on the total weight of the cosmetic composition as 0.40 wt%, 0.45 wt%, 0.48 wt%, 0.49 wt%, 0.50 wt%, 0.51 wt%, 0.52 wt%, 0.53 wt%, 0.54 wt%, 0.55 wt%, 0.60 wt%, or any value within a range of any two of these values.

[0033] In some embodiments, the content of the skin feel modifier, calculated based on the total weight of the cosmetic composition, is 9.00 wt%, 9.10 wt%, 9.20 wt%, 9.30 wt%, 9.40 wt%, 9.50 wt%, 9.60 wt%, 9.70 wt%, 9.80 wt%, 9.90 wt%, 10.00 wt%, or any value within a range of any two of these values.

[0034] In some embodiments, the content of the penetration enhancer is calculated based on the total weight of the cosmetic composition as 3.00 wt%, 3.10 wt%, 3.20 wt%, 3.30 wt%, 3.40 wt%, 3.50 wt%, or any value within the range of any two of these values.

[0035] In some embodiments, the content of the pH adjuster is calculated based on the total weight of the cosmetic composition as 0.02 wt%, 0.03 wt%, 0.04 wt%, or any value within a range of any two of these values.

[0036] In some embodiments, the solvent content, calculated based on the total weight of the cosmetic composition, is 70.00 wt%, 70.50 wt%, 71.00 wt%, 71.50 wt%, 72.00 wt%, 72.50 wt%, 73.00 wt%, 73.50 wt%, 74.00 wt%, 74.50 wt%, 75.00 wt%, or any value within a range of any two of these values.

[0037] In some embodiments, based on the total weight of the cosmetic composition, the content of the active ingredient is 0.08 wt% to 0.10 wt%, the content of other active ingredients is 0.30 wt% to 0.50 wt%, the content of the moisturizer is 5.50 wt% to 6.00 wt%, the content of the thickener is 0.40 wt% to 0.50 wt%, the content of the emulsifier is 6.00 wt% to 6.50 wt%, the content of the osmotic pressure regulator is 0.75 wt% to 0.85 wt%, the content of the antioxidant is 0.50 wt% to 0.60 wt%, the content of the skin feel modifier is 9.50 wt% to 10.00 wt%, the content of the penetration enhancer is 3.00 wt% to 3.50 wt%, the content of the pH adjuster is 0.02 wt% to 0.04 wt%, and the content of the solvent is 70.00 wt% to 75.00 wt%.

[0038] In some embodiments, based on the total weight of the cosmetic composition, the content of the active ingredient is 0.08 wt% to 0.10 wt%, the content of other active ingredients is 0.30 wt%, the content of the moisturizer is 5.56 wt%, the content of the thickener is 0.40 wt%, the content of the emulsifier is 6.30 wt%, the content of the osmotic pressure regulator is 0.80 wt%, the content of the antioxidant is 0.50 wt% to 0.51 wt%, the content of the skin feel modifier is 9.51 wt% to 9.20 wt%, the content of the penetration enhancer is 3.10 wt%, and the content of the pH adjuster is 0.03 wt%; the balance is the solvent.

[0039] In some embodiments, the cosmetic composition is a water-in-oil formulation, and the cosmetic composition is prepared from components A, B, C, and D.

[0040] in,

[0041] Based on the total weight of the cosmetic composition, component A comprises: 4.50wt%~5.50wt% of a first moisturizer, 0.70wt%~0.90wt% of sodium chloride, 9.00wt%~11.00wt% of 1,3-butanediol, 0.40wt%~0.55wt% of trehalose, and 52.00wt%~55.00wt% of water; the first moisturizer is composed of glycerin and sodium hyaluronate.

[0042] Based on the total weight of the cosmetic composition, component B comprises: 0.40wt%~0.55wt% p-hydroxyacetophenone, 1.00wt%~3.00wt% 1,3-butanediol, 0.02wt%~0.10wt% β-glucan, 0.20wt%~0.50wt% tetrahydromethylpyrimidine carboxylic acid, 2.00wt%~3.00wt% anti-allergen, 0.40wt%~0.70wt% 1,2-hexanediol, and 4.00wt%~6.00wt% water;

[0043] Based on the total weight of the cosmetic composition, component C comprises: 3.00wt%~4.00wt% polydimethylsiloxane, 0.10wt%~0.30wt% polydimethylsiloxane PEG-10 / 15 crosspolymer, 1.00wt%~2.00wt% cyclopentasiloxane, 0.30wt%~0.45wt% cyclohexylsiloxane, 2.00wt%~3.60wt% caprylic / capric triglyceride, and 0.30wt%~0.60wt% thickener;

[0044] Based on the total weight of the cosmetic composition, component D comprises: 2.00wt%~4.00wt% of a first emulsifier, 3.00wt%~5.00wt% of cyclopentasiloxane, 0.50wt%~1.50wt% of cyclohexylsiloxane, and 1.50wt%~3.00wt% of isononanoate; the first emulsifier comprises PEG-10 polydimethylsiloxane and lauryl PEG-9 polydimethylsiloxane ethyl polydimethylsiloxane;

[0045] The anti-allergy agent is composed of CTR50 and Anoectochilus roxburghii extract;

[0046] Based on the total weight of the CTR50, the CTR50 comprises the following weight percentages: 4.00wt%~6.00wt% of Corydalis yanhusuo root extract, 75.00wt%~85.00wt% of 1,3-butanediol, 2.50wt%~4.00wt% of citric acid, and 8.00wt%~12.00wt% of isosorbide dimethyl ether;

[0047] Based on the total weight of the *Anoectochilus roxburghii* extract, the extract comprises the following weight percentages: 2.00wt%~4.00wt% *Anoectochilus roxburghii* extract, 0.40wt%~0.60wt% p-hydroxyacetophenone, 8.00wt%~12.00wt% 1,3-butanediol, 0.80wt%~1.50wt% 1,2-hexanediol, 0.05wt%~0.20wt% ethylhexylglycerin, and 83.00wt%~87.00wt% water.

[0048] In some embodiments, the Anoectochilus roxburghii extract in the cosmetic composition is provided by the Anoectochilus roxburghii extract in the Anoectochilus roxburghii extract solution, and the Corydalis yanhusuo root extract is provided by the Corydalis yanhusuo root extract in the CTR50.

[0049] In some embodiments, based on the total weight of the cosmetic composition, the content of the first moisturizer in component A in the cosmetic composition is 4.50 wt%, 4.60 wt%, 4.70 wt%, 4.80 wt%, 4.90 wt%, 5.00 wt%, 5.03 wt%, 5.10 wt%, 5.20 wt%, 5.30 wt%, 5.40 wt%, 5.50 wt%, or any value within a range of any two of these values.

[0050] In some embodiments, based on the total weight of the cosmetic composition, the content of sodium chloride in component A in the cosmetic composition is 0.70 wt%, 0.75 wt%, 0.80 wt%, 0.85 wt%, 0.90 wt%, or any value within a range of any two of these values.

[0051] In some embodiments, based on the total weight of the cosmetic composition, the content of 1,3-butanediol in component A in the cosmetic composition is 9.00 wt%, 9.50 wt%, 10.00 wt%, 10.50 wt%, 11.00 wt%, or any value within a range of any two of these values.

[0052] In some embodiments, based on the total weight of the cosmetic composition, the content of trehalose in component A in the cosmetic composition is 0.40 wt%, 0.45 wt%, 0.50 wt%, 0.55 wt%, or any value within a range of any two of these values.

[0053] In some embodiments, based on the total weight of the cosmetic composition, the water content of component A in the cosmetic composition is 52.00 wt%, 53.00 wt%, 54.00 wt%, 54.04 wt%, 54.10 wt%, 54.20 wt%, 54.24 wt%, 54.30 wt%, 54.40 wt%, 54.44 wt%, 54.50 wt%, 54.60 wt%, 54.64 wt%, 54.70 wt%, 55.00 wt%, or any value within a range of any two of these values.

[0054] In some embodiments, based on the total weight of the cosmetic composition, the content of p-hydroxyacetophenone in component B in the cosmetic composition is 0.40 wt%, 0.45 wt%, 0.50 wt%, 0.55 wt%, or any value within a range of any two of these values.

[0055] In some embodiments, based on the total weight of the cosmetic composition, the content of 1,3-butanediol in component B in the cosmetic composition is 1.00 wt%, 1.50 wt%, 2.00 wt%, 2.50 wt%, 3.00 wt%, or any value within a range of any two of these values.

[0056] In some embodiments, based on the total weight of the cosmetic composition, the content of β-glucan in component B in the cosmetic composition is 0.02wt%, 0.03wt%, 0.04wt%, 0.05wt%, 0.06wt%, 0.07wt%, 0.08wt%, 0.09wt%, 0.10wt%, or any value within a range of any two of these values.

[0057] In some embodiments, based on the total weight of the cosmetic composition, the content of tetrahydromethylpyrimidine carboxylic acid in component B in the cosmetic composition is 0.20 wt%, 0.25 wt%, 0.30 wt%, 0.35 wt%, 0.40 wt%, 0.45 wt%, 0.50 wt%, or any value within a range of any two of these values.

[0058] In some embodiments, based on the total weight of the cosmetic composition, the content of the anti-allergen in component B in the cosmetic composition is 2.00 wt%, 2.20 wt%, 2.40 wt%, 2.60 wt%, 2.80 wt%, 3.00 wt%, or any value within a range of any two of these values.

[0059] In some embodiments, based on the total weight of the cosmetic composition, the content of 1,2-hexanediol in component B in the cosmetic composition is 0.40 wt%, 0.45 wt%, 0.50 wt%, 0.55 wt%, 0.60 wt%, 0.65 wt%, 0.70 wt%, or any value within a range of any two of these values.

[0060] In some embodiments, based on the total weight of the cosmetic composition, the water content in component B in the cosmetic composition is 4.00 wt%, 4.50 wt%, 5.00 wt%, 5.50 wt%, 6.00 wt%, or any value within a range of any two of these values.

[0061] In some embodiments, based on the total weight of the cosmetic composition, the content of polydimethylsiloxane in component C in the cosmetic composition is 3.00 wt%, 3.50 wt%, 3.60 wt%, 3.70 wt%, 3.80 wt%, 3.90 wt%, 4.00 wt%, or any value within a range of any two of these values.

[0062] In some embodiments, based on the total weight of the cosmetic composition, the content of the polydimethylsiloxane PEG-10 / 15 crosspolymer in component C in the cosmetic composition is 0.10 wt%, 0.15 wt%, 0.20 wt%, 0.25 wt%, 0.30 wt%, or any value within a range of any two of these values.

[0063] In some embodiments, based on the total weight of the cosmetic composition, the content of cyclopentadimethylsiloxane in component C in the cosmetic composition is 1.00 wt%, 1.50 wt%, 1.60 wt%, 1.70 wt%, 1.80 wt%, 1.90 wt%, 2.00 wt%, or any value within a range of any two of these values.

[0064] In some embodiments, based on the total weight of the cosmetic composition, the content of cyclohexylsiloxane in component C in the cosmetic composition is 0.30 wt%, 0.35 wt%, 0.40 wt%, 0.45 wt%, or any value within a range of any two of these values.

[0065] In some embodiments, based on the total weight of the cosmetic composition, the content of caprylic / capric triglycerides in component C in the cosmetic composition is 2.00 wt%, 2.50 wt%, 3.00 wt%, 3.50 wt%, 3.60 wt%, or any value within a range of any two of these values.

[0066] In some embodiments, based on the total weight of the cosmetic composition, the content of the thickener in component C in the cosmetic composition is 0.30 wt%, 0.35 wt%, 0.40 wt%, 0.45 wt%, 0.50 wt%, 0.55 wt%, 0.60 wt%, or any value within a range of any two of these values.

[0067] In some embodiments, based on the total weight of the cosmetic composition, the content of the first emulsifier in component D in the cosmetic composition is 2.00 wt%, 2.50 wt%, 3.00 wt%, 3.50 wt%, 4.00 wt%, or any value within a range of any two of these values.

[0068] In some embodiments, based on the total weight of the cosmetic composition, the content of cyclopentadimethylsiloxane in component D in the cosmetic composition is 3.00 wt%, 3.50 wt%, 4.00 wt%, 4.50 wt%, 5.00 wt%, or any value within a range of any two of these values.

[0069] In some embodiments, based on the total weight of the cosmetic composition, the content of cyclohexylsiloxane in component D in the cosmetic composition is 0.50 wt%, 1.00 wt%, 1.50 wt%, or any value within a range of any two of these values.

[0070] In some embodiments, based on the total weight of the cosmetic composition, the content of isononyl isononanoate in component D in the cosmetic composition is 1.50 wt%, 2.00 wt%, 2.50 wt%, 3.00 wt%, or any value within a range of any two of these values.

[0071] In some embodiments, based on the total weight of the cosmetic composition, the glycerin content of the first moisturizer is 4.48wt% to 5.45wt%, and the sodium hyaluronate content of the first moisturizer is 0.02wt% to 0.05wt%.

[0072] In some embodiments, based on the total weight of the cosmetic composition, the glycerin content of the first moisturizer is 4.48 wt%, 4.50 wt%, 5.00 wt%, 5.10 wt%, 5.20 wt%, 5.30 wt%, 5.40 wt%, 5.45 wt%, or any value within a range of any two of these values.

[0073] In some embodiments, the sodium hyaluronate content of the first moisturizer is calculated based on the total weight of the cosmetic composition as 0.02 wt%, 0.03 wt%, 0.04 wt%, 0.05 wt%, or any value within a range of any two of these values.

[0074] In some embodiments, based on the total weight of the cosmetic composition, the content of PEG-10 polydimethylsiloxane is 1.30wt%~2.50wt%, and the content of lauryl PEG-9 polydimethylsiloxane is 0.70wt%~1.50wt%.

[0075] In some embodiments, the content of PEG-10 polydimethylsiloxane, calculated based on the total weight of the cosmetic composition, is 1.30 wt%, 1.40 wt%, 1.50 wt%, 1.60 wt%, 1.70 wt%, 1.80 wt%, 1.90 wt%, 2.00 wt%, 2.50 wt%, or any value within a range of any two of these values.

[0076] In some embodiments, the content of lauryl PEG-9 polydimethylsiloxane is calculated based on the total weight of the cosmetic composition as 0.70 wt%, 0.80 wt%, 0.90 wt%, 1.00 wt%, 1.10 wt%, 1.20 wt%, 1.30 wt%, 1.40 wt%, 1.50 wt%, or any value within the range of any two of these values.

[0077] In some embodiments, the content of the Corydalis rhizome extract in the CTR50, calculated based on the total weight of the CTR50, is 4.00 wt%, 4.50 wt%, 5.00 wt%, 5.50 wt%, 6.00 wt%, or any value within a range of any two of these values.

[0078] In some embodiments, the content of 1,3-butanediol in the CTR50, calculated based on the total weight of the CTR50, is 75.00 wt%, 76.00 wt%, 77.00 wt%, 78.00 wt%, 79.00 wt%, 80.00 wt%, 81.00 wt%, 82.00 wt%, 83.00 wt%, 84.00 wt%, 85.00 wt%, or any value within a range of any two of these values.

[0079] In some embodiments, the citric acid content in the CTR50 is 2.50 wt%, 3.00 wt%, 3.50 wt%, 4.00 wt%, or any value within a range of any two of these values, calculated based on the total weight of the CTR50.

[0080] In some embodiments, the content of isosorbide dimethyl ether in the CTR50, calculated based on the total weight of the CTR50, is 8.00 wt%, 8.50 wt%, 9.00 wt%, 9.50 wt%, 10.00 wt%, 10.50 wt%, 11.00 wt%, 11.50 wt%, 12.00 wt%, or any value within a range of any two of these values.

[0081] In some embodiments, the CTR50 is composed of the following weight percentages, calculated based on the total weight of the CTR50: 5.00 wt% Corydalis yanhusuo root extract, 82.00 wt% 1,3-butanediol, 3.00 wt% citric acid, and 10.00 wt% isosorbide dimethyl ether.

[0082] In some embodiments, the content of the *Anoectochilus roxburghii* extract in the *Anoectochilus roxburghii* extract is 2.00 wt%, 2.50 wt%, 3.00 wt%, 3.50 wt%, 4.00 wt%, or any value within the range of any two of these values, calculated based on the total weight of the *Anoectochilus roxburghii* extract.

[0083] In some embodiments, the content of p-hydroxyacetophenone in the *Anoectochilus roxburghii* extract is 0.40 wt%, 0.45 wt%, 0.50 wt%, 0.55 wt%, 0.60 wt%, or any value within the range of any two of these values, calculated based on the total weight of the extract.

[0084] In some embodiments, the content of 1,3-butanediol in the *Anoectochilus roxburghii* extract is 8.00 wt%, 8.50 wt%, 9.00 wt%, 9.50 wt%, 10.00 wt%, 10.50 wt%, 11.00 wt%, 11.50 wt%, 12.00 wt%, or any value within a range of any two of these values, calculated based on the total weight of the *Anoectochilus roxburghii* extract.

[0085] In some embodiments, the content of 1,2-hexanediol in the *Anoectochilus roxburghii* extract is 0.80 wt%, 0.90 wt%, 1.00 wt%, 1.10 wt%, 1.20 wt%, 1.30 wt%, 1.40 wt%, 1.50 wt%, or any value within the range of any two of these values, calculated based on the total weight of the *Anoectochilus roxburghii* extract.

[0086] In some embodiments, the ethylhexylglycerin is 0.05wt%, 0.10wt%, 0.15wt%, 0.20wt%, or any value within a range of any two of these values, calculated based on the total weight of the *Anoectochilus roxburghii* extract.

[0087] In some embodiments, the water content in the *Anoectochilus roxburghii* extract is 83.00 wt%, 84.00 wt%, 85.00 wt%, 85.40 wt%, 86.00 wt%, 87.00 wt%, or any value within a range of any two of these values, calculated based on the total weight of the extract.

[0088] In some embodiments, the extract is composed of the following weight percentages, calculated based on the total weight of the *Anoectochilus roxburghii* extract: 3.00 wt% *Anoectochilus roxburghii* extract, 0.50 wt% p-hydroxyacetophenone, 10.00 wt% 1,3-butanediol, 1.00 wt% 1,2-hexanediol, 0.10 wt% ethylhexylglycerin, and 85.40 wt% water.

[0089] In some embodiments, the method for preparing the CTR50 includes:

[0090] S1: Mix 1,3-butanediol with 2 to 3 times the weight of citric acid, heat to 50 to 60°C to dissolve, and obtain mixture 1;

[0091] S2: Take the remaining 1,3-butanediol, add the Corydalis yanhusuo root extract, heat to 110~120℃, stir to dissolve, and obtain mixture 2;

[0092] S3: When the temperature of the mixture 2 obtained in step S2 drops to 90~95℃, add the mixture 1 obtained in step S1 to the mixture 2 and stir evenly to obtain the mixture 3.

[0093] S4: Isosorbide dimethyl ether was added to the cooled mixture 3, mixed evenly, and homogenized by high-pressure microjet to obtain CTR50;

[0094] In some embodiments, the method for preparing the *Anoectochilus roxburghii* extract includes:

[0095] (i) Mix p-hydroxyacetophenone with 1,3-butanediol and heat to 65~75℃ to disperse evenly to obtain mixture 4;

[0096] (ii) Cool the mixture 4 obtained in step (i) to 45~65℃, add ethylhexylglycerol that has been dispersed in 1,2-hexanediol, stir evenly to obtain mixture 5;

[0097] (iii) When the temperature of the mixture 5 obtained in step (ii) drops to 45°C or below, add water and Anoectochilus roxburghii extract, mix well, and obtain Anoectochilus roxburghii extract.

[0098] Secondly, the present invention provides a method for preparing the aforementioned cosmetic composition.

[0099] A method for preparing the cosmetic composition according to the first aspect, the method comprising:

[0100] (1) Mix the components in component A and heat to 85~90℃ to dissolve them to obtain the first mixture;

[0101] (2) Cool the temperature of the first mixture to 45~65℃ (e.g., 45℃, 50℃, 55℃, 60℃, 65℃ or any value between any two of these), add a 1,3-butanediol solution of p-hydroxyacetophenone, then add an aqueous solution of β-glucan and tetrahydromethylpyrimidine carboxylic acid, and stir; cool to 25℃~45℃ (e.g., 25℃, 30℃, 35℃, 40℃, 45℃ or any value between any two of these), then mix with the other components in component B, and stir to obtain the second mixture;

[0102] (3) Mix the polydimethylsiloxane PEG-10 / 15 cross-linked polymer, cyclopentadimethylsiloxane and cyclohexylsiloxane in component C evenly, then add the first thickener that has been homogenized and dispersed with glycerol caprylic / capric acid and polydimethylsiloxane, stir evenly, then add the components in component D, stir, and obtain an oily mixture.

[0103] (4) Under stirring conditions of 100 rpm to 200 rpm (e.g., 100 rpm, 120 rpm, 150 rpm, 170 rpm, 200 rpm or any value within any two of these ranges), slowly add the second mixture to the oily mixture. After the addition is complete, increase the speed to 400 rpm to 500 rpm (e.g., 400 rpm, 420 rpm, 450 rpm, 470 rpm, 500 rpm or any value within any two of these ranges) and stir for 5 to 10 minutes (e.g., 5 minutes, 6 minutes, 7 minutes, 8 minutes, 9 minutes, 10 minutes or any value within any two of these ranges) to make the system initially uniform. Homogenize at 2000 rpm to 3000 rpm (e.g., 2000 rpm, 2200 rpm, 2500 rpm, 2700 rpm, 2900 rpm, 3000 rpm or any value within any two of these ranges) for 2 to 5 minutes. The cosmetic composition is obtained by applying the emulsion to a uniform, smooth, glossy, and stable emulsion state for a period of time (e.g., 2 min, 3 min, 4 min, 5 min, or any value within any two of these ranges) until the emulsion exhibits a stable emulsion state.

[0104] Thirdly, the present invention provides an application of the aforementioned cosmetic composition or the cosmetic composition prepared by the aforementioned preparation method.

[0105] The use of a cosmetic composition as described in the first aspect or a cosmetic composition prepared by the preparation method described in the second aspect in the preparation of cosmetics for skin soothing and / or repair.

[0106] In some embodiments, the cosmetic is used to soothe and / or repair at least one of rosacea, atopic dermatitis, and sensitive skin.

[0107] Beneficial effects

[0108] Compared with the prior art, a certain embodiment of the present invention has at least one of the following beneficial technical effects:

[0109] (1) The cosmetic composition provided by the present invention has the effects of reducing capillary dilation, reducing inflammation and erythema.

[0110] (2) The cosmetic composition provided by the present invention has the effects of reducing inflammatory response, alleviating local edema and erythema, and quickly relieving symptoms such as itching and edema.

[0111] (3) The cosmetic composition provided by the present invention can effectively reduce facial erythema and papules in a very short time (3 min), reduce redness, and has the effects of immediate redness reduction, swelling reduction, soothing and repair.

[0112] (4) The extracts of Anoectochilus roxburghii and Corydalis yanhusuo are the core substances that achieve excellent redness relief and soothing effects. Both are indispensable. The combined use of Anoectochilus roxburghii extract and Corydalis yanhusuo extract has a significantly higher effect than the additive effect of single active ingredients, and has a significant synergistic effect.

[0113] (5) The present invention can significantly reduce the transepidermal water loss of the skin and enhance the skin barrier function by synergistic combination of Anoectochilus roxburghii extract and Corydalis yanhusuo root extract, and is suitable for repairing skin barrier damage, dryness and sensitivity.

[0114] (6) When the extracts of Anoectochilus roxburghii and Corydalis yanhusuo were combined in different proportions, they produced a clear synergistic effect. Their hyaluronidase inhibition rate was significantly higher than the additive effect of the single active ingredients, and the inhibition effect increased in a dose-dependent manner with the increase of Anoectochilus roxburghii extract.

[0115] (7) Compared with other emulsifiers, the emulsifier in component C of the present invention is preferably polydimethylsiloxane and polydimethylsiloxane PEG-10 / 15 cross-linked polymer, which is more conducive to improving the stability of the obtained cosmetic composition and has unexpected technical effects.

[0116] (8) Compared with other emulsifiers, the emulsifier in component D of the present invention preferably includes lauryl PEG-9 polydimethylsiloxane, which is more conducive to improving the stability of the obtained cosmetic composition and has unexpected technical effects.

[0117] (9) Compared with other thickeners, the present invention preferably uses distearate dimethylammonium lithium montmorillonite as a thickener, which is more conducive to improving the stability of the obtained cosmetic composition and has unexpected technical effects.

[0118] (10) The present invention preferably uses polydimethylsiloxane and polydimethylsiloxane PEG-10 / 15 cross-linked polymer as emulsifier in component C, preferably including lauryl PEG-9 polydimethylsiloxane ethyl polydimethylsiloxane, and preferably uses distearate dimethylammonium lithium montmorillonite as thickener. These preferred excipients synergistically improve the stability of the obtained cosmetic composition and have unexpected technical effects. These preferred excipients are indispensable. It is difficult to improve the stability of the obtained cosmetic composition by replacing them with other excipients with similar structures or functions.

[0119] Terminology definition:

[0120] In this invention, "room temperature" refers to the ambient temperature, which can be 20℃-30℃; in some embodiments, it is 22℃-28℃; in some embodiments, it is 24℃-26℃; and in some embodiments, it is 25℃.

[0121] In the foregoing description of this invention, all figures disclosed herein, whether or not the words “approximately” or “about” are used, are approximate values. Based on the disclosed figures, the value of each figure may vary by less than ±10% or by a difference that is considered reasonable by those skilled in the art, such as ±1%, ±2%, ±3%, ±4%, or ±5%.

[0122] The terms “optional,” “optional,” or “optionally” mean that the event or situation described below may, but is not necessarily, occur. For example, “optional surfactant” means that the surfactant may or may not be present.

[0123] The term “and / or” should be understood to mean any one of the options or any combination of two or more of the options.

[0124] “wt%” indicates weight percentage.

[0125] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples. Attached Figure Description

[0126] Figure 1 is a comparison of subject 5, a patient with rosacea in Test Example 1, before and 7 days after using the cosmetic composition product obtained in Example 4.

[0127] Figure 2 is a comparison of subject 8, a patient with rosacea in Test Example 1, before and 48 hours after using the cosmetic composition product obtained in Example 4.

[0128] Figure 3 is a comparison of subject 10, a patient with rosacea in Test Example 1, before and 7 days after using the cosmetic composition product obtained in Example 4.

[0129] Figure 4 is a comparison of subject 13, a patient with rosacea in Test Example 1, before and 7 days after using the cosmetic composition product obtained in Example 4.

[0130] Figure 5 is a comparison of the views of subject 10, a patient with atopic dermatitis in Test Example 2, before and 7 days after using the cosmetic composition product obtained in Example 4.

[0131] Figure 6 is a left-side view comparison of subject 10, a patient with atopic dermatitis in Test Example 2, before and 7 days after using the cosmetic composition product obtained in Example 4.

[0132] Figure 7 is a left-side view comparison of subject 16, a patient with atopic dermatitis in Test Example 2, before and 7 days after using the cosmetic composition product obtained in Example 4.

[0133] Figure 8 shows a comparison of VISIA skin images of sensitive patient subject 12 in Test Example 3 before and 3 minutes after using the cosmetic composition product obtained in Example 4.

[0134] Figure 9 shows a comparison of VISIA skin images of sensitive patient subject No. 18 in Test Example 3 before and 3 minutes after using the cosmetic composition product obtained in Example 4. Detailed Implementation

[0135] To enable those skilled in the art to better understand the technical solutions of the present invention, some non-limiting embodiments are further disclosed below to provide a more detailed description of the present invention.

[0136] All reagents used in this invention can be purchased commercially or prepared by the methods described in this invention.

[0137] Experimental methods not specified in the examples are generally performed using methods known in the art.

[0138] I. The reagents used in the embodiments of the present invention can be obtained from conventional commercially available products or from the following sources or prepared by the following methods.

[0139] (1) Corydalis root extract

[0140] Preparation method of Corydalis yanhusuo root extract:

[0141] The crude powder of Corydalis rhizome was soaked in ethanol and then refluxed for extraction. The extract was then evaporated and concentrated until viscous, and then dried under vacuum to obtain the Corydalis rhizome extract.

[0142] (2) CTR50

[0143] Based on the total weight of the CTR50, the CTR50 is composed of the following weight percentages: 5.00 wt% Corydalis yanhusuo root extract, 82.00 wt% 1,3-butanediol (solvent), 3.00 wt% citric acid (pH adjuster), and 10.00 wt% isosorbide dimethyl ether (penetration enhancer).

[0144] The preparation methods of CTR50 include:

[0145] S1: Mix 1,3-butanediol with 2 to 3 times the weight of citric acid, heat to 50 to 60°C to dissolve, and obtain mixture 1;

[0146] S2: Take the remaining 1,3-butanediol, add the Corydalis yanhusuo root extract, heat to 110~120℃, stir to dissolve, and obtain mixture 2;

[0147] S3: When the temperature of the mixture 2 obtained in step S2 drops to 90~95℃, add the mixture 1 obtained in step S1 to the mixture 2 and stir evenly to obtain the mixture 3.

[0148] S4: Isosorbide dimethyl ether is added to the cooled mixture 3, mixed evenly, and homogenized by high-pressure microfluidic jet to obtain CTR50.

[0149] (3) Extract of Anoectochilus roxburghii

[0150] Preparation method of Anoectochilus roxburghii extract:

[0151] The dried whole plant of *Anoectochilus roxburghii* (artificially cultivated) was pulverized to obtain *Anoectochilus roxburghii* powder; then the *Anoectochilus roxburghii* powder was mixed with water, centrifuged, the supernatant was collected, concentrated, and freeze-dried to obtain the *Anoectochilus roxburghii* extract.

[0152] (4) Extract of Anoectochilus roxburghii

[0153] Based on the total weight of the *Anoectochilus roxburghii* extract, the extract comprises the following weight percentages: 3.00 wt% *Anoectochilus roxburghii* extract, 0.50 wt% p-hydroxyacetophenone (antioxidant), 10.00 wt% 1,3-butanediol (solvent), 1.00 wt% 1,2-hexanediol (skin feel modifier), 0.10 wt% ethylhexylglycerin (skin feel modifier), and 85.40 wt% water (solvent).

[0154] The preparation method of the *Anoectochilus roxburghii* extract includes:

[0155] (i) Mix p-hydroxyacetophenone with 1,3-butanediol and heat to 65~75℃ to disperse evenly to obtain mixture 4;

[0156] (ii) Cool the mixture 4 obtained in step (i) to 45~65℃, add ethylhexylglycerol that has been dispersed in 1,2-hexanediol, stir evenly to obtain mixture 5;

[0157] (iii) When the temperature of the mixture 5 obtained in step (ii) drops to 45°C or below, add water and Anoectochilus roxburghii extract, mix well, and obtain Anoectochilus roxburghii extract.

[0158] Example 1: A water-in-oil soothing and repairing composition containing Anoectochilus roxburghii extract (hereinafter referred to as: cosmetic composition)

[0159] Formula: See Table 1.

[0160]

[0161] Preparation method:

[0162] (1) Mix the components in component A and heat to 85°C (85°C~90°C) to dissolve them, to obtain the first mixture;

[0163] (2) Cool the temperature of the first mixture to 50°C (or 45~65°C), add a 1,3-butanediol solution of p-hydroxyacetophenone, then add an aqueous solution of β-glucan and tetrahydromethylpyrimidine carboxylic acid, and stir; cool to 45°C (or 25°C~45°C), then mix with the other components in component B, and stir to obtain the second mixture;

[0164] (3) Mix the polydimethylsiloxane PEG-10 / 15 cross-linked polymer, cyclopentadimethylsiloxane and cyclohexylsiloxane in component C evenly, then add the first thickener that has been homogenized and dispersed with glycerol caprylic / capric acid and polydimethylsiloxane, stir evenly, then add the components in component D, stir, and obtain an oily mixture.

[0165] (4) Under the stirring condition of 150 rpm, the second mixture is slowly added to the oily mixture. After the addition is completed, the speed is increased to 400 rpm and stirred for 5 min (5 min to 10 min) to make the system initially mixed evenly. Then, it is homogenized at 2000 rpm (2000 rpm to 3000 rpm) for 2 min (2 min to 5 min) to obtain the cosmetic composition (the material presents a uniform, delicate, glossy, and stable emulsion state).

[0166] Example 2: A water-in-oil soothing and repairing composition containing Anoectochilus roxburghii extract (hereinafter referred to as: cosmetic composition)

[0167] Example 2: The only difference from Example 1 is that the amount of Anoectochilus roxburghii extract added is adjusted to 1.20 wt%, and the water in component B is appropriately reduced so that the total content of each component in the cosmetic composition is 100 wt%. The other conditions are the same as in Example 1.

[0168] Example 3: A water-in-oil soothing and repairing composition containing Anoectochilus roxburghii extract (hereinafter referred to as: cosmetic composition)

[0169] Example 3: The only difference from Example 1 is that the amount of Anoectochilus roxburghii extract added is adjusted to 1.40 wt%, and the water in component B is appropriately reduced so that the total content of each component in the cosmetic composition is 100 wt%. The other conditions are the same as in Example 1.

[0170] Example 4: A water-in-oil soothing and repairing composition containing Anoectochilus roxburghii extract (hereinafter referred to as: cosmetic composition)

[0171] Example 4: The only difference from Example 1 is that the amount of Anoectochilus roxburghii extract added is adjusted to 1.60 wt%, and the water in component B is appropriately reduced so that the total content of each component in the cosmetic composition is 100 wt%. The other conditions are the same as in Example 1.

[0172] Comparative Example 1: Extract of *Anoectochilus roxburghii* lacking

[0173] Comparative Example 1: The only difference from Example 4 is that the amount of Anoectochilus roxburghii extract added is adjusted to 0 wt%, and the water in component B is appropriately increased so that the total content of each component in the cosmetic composition is 100 wt%. The other conditions are the same as in Example 4.

[0174] Comparative Example 2: Missing CTR50

[0175] Comparative Example 2: The only difference from Example 4 is that the amount of CTR50 added was adjusted to 0 wt%, and the water in component B was appropriately increased so that the total content of each component in the cosmetic composition was 100 wt%. All other conditions were the same as in Example 4.

[0176] Comparative Example 3: *Anoectochilus roxburghii* extract and CTR50 were missing.

[0177] Comparative Example 3: The only difference from Example 4 is that the amount of CTR50 and Anoectochilus roxburghii extract added is adjusted to 0 wt%, and the water in component B is appropriately increased so that the total content of each component in the cosmetic composition is 100 wt%. The other conditions are the same as in Example 4.

[0178] Test Example 1: Barrier Repair and Redness Reduction Function

[0179] The cosmetic composition prepared in Example 4 was evaluated for its barrier repair and redness-reducing functions. Referring to the "Chinese Guidelines for the Diagnosis and Treatment of Rosacea (2021 Edition)," 35 subjects with clinical manifestations meeting the diagnostic criteria for rosacea were randomly selected, with an average age of 35 years. The cosmetic composition prepared in Example 4 was applied to the affected area twice daily, morning and evening, and changes in the redness area on the face were observed before and after use.

[0180] Experimental results: Representative results are shown in Figures 1 to 4.

[0181] Results analysis: After subject 8 used the cosmetic composition prepared in Example 4 of this invention for 48 hours, and subjects 5, 10 and 13 used the cosmetic composition prepared in Example 4 of this invention for 7 days, the facial redness of the subjects was significantly improved, and the papules, itching and stinging sensation were significantly reduced. The results prove that the cosmetic composition provided by this invention has the effect of reducing capillary dilation and alleviating inflammation and erythema.

[0182] Test Example 2: Anti-inflammatory and soothing

[0183] The anti-inflammatory and soothing functions of the cosmetic composition prepared in Example 4 were evaluated. Referring to the "Guidelines for the Diagnosis and Treatment of Atopic Dermatitis in Primary Care (2022 Edition)," 35 subjects with clinical manifestations meeting the diagnostic criteria for atopic dermatitis were randomly selected, with an average age of 35 years. The cosmetic composition prepared in Example 4 was applied to the affected area twice daily, morning and evening. Changes in redness on the face and arms were observed after using the water-in-oil soothing and repairing composition.

[0184] Experimental results: Representative results are shown in Figures 5 to 7.

[0185] Results analysis: After using the cosmetic composition provided by the present invention for 7 days, the erythematous papules and scales of subjects No. 10 and No. 16 were significantly reduced, and the itching and burning pain were significantly relieved. The results prove that the cosmetic composition provided by the present invention has the effects of reducing inflammatory response, reducing local edema and erythema, and quickly relieving symptoms such as itching and edema.

[0186] Test Example 3: Immediate reduction of redness and swelling, soothing and repair

[0187] The anti-inflammatory and soothing functions of the cosmetic composition prepared in Example 4 were evaluated. Referring to the group standard T / CAB 0152-2022 "Test Methods for Seven Efficacy Items of Cosmetics: Anti-wrinkle, Firming, Moisturizing, Oil Control, Repairing, Nourishing, and Soothing", 35 subjects clinically diagnosed with sensitive skin (mean age 35 years) were randomly recruited. The changes in facial areas of the subjects before and 3 minutes after using the cosmetic composition were analyzed using the VISIA skin image analysis instrument.

[0188] Experimental results: Representative results are shown in Figures 8 and 9.

[0189] Results analysis:

[0190] (1) After using the cosmetic composition provided by the present invention for 3 minutes, the erythema and papules on the cheeks of subjects 12 and 18 were reduced. The area of ​​erythema on subject 12 was reduced and the color was lighter; the number of papules was also reduced. The overall redness on the face of subject 18 was reduced and the skin color appeared more even.

[0191] (2) The results show that the cosmetic composition provided by the present invention can effectively reduce facial erythema and papules in a very short time (3 min), reduce redness, and has the effects of immediate redness reduction, swelling reduction, soothing and repair.

[0192] Test Example 4:

[0193] The cosmetic compositions prepared in Examples 1-4 and Comparative Examples 1-3 were evaluated for their soothing efficacy. Referring to group standard T / CNMIA 0015-2020, the skin hemoglobin (EI) content of women aged 18-55 with dry and rough facial skin was measured before and after product use using a melanin and hemoglobin content analyzer (Mexameter MX1). The cosmetic composition prepared in Example 4 was applied to the affected area twice daily, morning and evening. Changes in skin hemoglobin content before and after product use are shown in Table 2.

[0194]

[0195] Note: * indicates a significant improvement compared to before product use (p<0.05), and ** indicates a highly significant improvement (p<0.01).

[0196] in conclusion:

[0197] (1) As shown in Table 2, the cosmetic compositions prepared in Examples 1-4 can significantly reduce the hemoglobin content in the skin, and the effect increases in a dose-dependent manner with the increase of Anoectochilus roxburghii extract. Among them, Example 4 has the best soothing and redness-reducing effect.

[0198] (2) Comparative Example 3 had no obvious soothing and redness-reducing effect. Compared with Comparative Example 1, Comparative Example 2 reduced the skin hemoglobin content to a greater extent than Comparative Example 1, and the soothing and redness-reducing effects of Comparative Examples 1-2 were all significantly weaker than those of Examples 1-4.

[0199] (3) Experimental results show that *Anoectochilus roxburghii* extract and *Corydalis yanhusuo* root extract are the core substances for achieving excellent redness-reducing and soothing effects. Neither can be omitted. The combined effect of *Anoectochilus roxburghii* extract and *Corydalis yanhusuo* root extract is significantly higher than the additive effect of a single active ingredient, exhibiting a significant synergistic effect. Therefore, the cosmetic compositions prepared in Examples 1-4 can significantly reduce the hemoglobin content in the skin and effectively improve skin erythema, redness, and other inflammatory conditions.

[0200] Test Example 5: Evaluation of Barrier Repair Function

[0201] The cosmetic compositions prepared in Examples 1-4 and Comparative Examples 1-3 were evaluated for their skin barrier repair function. Referring to industry standard QB / T 4256-2011, the loss of skin surface moisture in women aged 18-55 with dry and rough facial skin was measured before and after product use using a Tewameter TM300 (TeWL skin moisture loss tester). The cosmetic composition prepared in Example 4 was applied to the affected area twice daily, morning and evening. The changes in skin surface moisture loss before and after product use are shown in Table 3.

[0202]

[0203] Note: * indicates a significant improvement compared to before product use (p<0.05), and ** indicates a highly significant improvement (p<0.01).

[0204] in conclusion:

[0205] (1) As shown in Table 3, the transdermal water loss (TWEL) range of the cosmetic compositions prepared in Examples 1-4 is 12.76 g / (h·m 2 ~14.78 g / (h·m 2As the amount of Anoectochilus roxburghii extract in the cosmetic composition increases, the transepidermal water loss (TWEL) of the skin decreases accordingly for the same number of days of use.

[0206] (2) Compared with Examples 1-4, Comparative Example 3 had no significant effect on repairing the skin barrier; the transdermal water loss of Comparative Examples 2 and 3 was 17.37 g / (h·m). 2 ) and 16.01 g / (h·m 2 The skin barrier repair effects of the samples in Examples 1-4 were significantly lower than those in Examples 1-4. The results indicate that the cosmetic compositions containing *Anoectochilus roxburghii* extract prepared in Examples 1-4, through the synergistic combination of *Anoectochilus roxburghii* extract and *Corydalis yanhusuo* root extract, can significantly reduce transepidermal water loss and enhance skin barrier function, making them suitable for repairing damaged skin barriers, dryness, and sensitivity.

[0207] Test Example 6: Examination of Anti-allergic Activity and Efficacy

[0208] Experimental Principle: Hyaluronidase is a major factor causing allergic reactions. When an allergen first enters the body, the body produces IgE antibodies. When the allergen re-enters the body, it binds to the IgE antibodies on the surface of mast cells, thereby triggering activation signals and releasing histamine. Histamine, under the activation of hyaluronidase, produces an allergic reaction. Therefore, inhibiting hyaluronidase activity can effectively prevent allergic reactions. Hyaluronidase is strongly correlated with inflammatory responses and allergic reactions. Anti-allergic activity is measured by the hyaluronidase inhibition rate; the higher the inhibition rate, the stronger the anti-allergic activity.

[0209] Experimental Methods: The anti-inflammatory efficacy of the cosmetic compositions prepared in Examples 1-4 and Comparative Examples 1-3 was evaluated. The hyaluronidase inhibition rate of the cosmetic compositions was determined according to relevant literature methods. Phosphate-buffered saline (PBS) (20 mmol / L, pH 7.0, containing 77 mmol / L NaCl), acidic bovine serum albumin (BSA) solution (containing 24 mmol / L anhydrous sodium acetate, 79 mmol / L glacial acetic acid, and 0.1% BSA, pH 3.75), sodium hyaluronate solution (0.5 mg / mL, containing 0.3 mol / L sodium dihydrogen phosphate, pH 5.35), and hyaluronidase solution (10 U / mL, prepared in 0.01% PBS) were prepared separately. All solutions were refrigerated and used immediately. For the cosmetic compositions prepared in Examples 1-4 and Comparative Examples 1-3, a 10 mg / mL sample solution was prepared using PBS.

[0210] Add 30 μL of sample solution and 30 μL of hyaluronidase solution sequentially to a 96-well plate, incubate at 37°C for 10 min, then add 30 μL of sodium hyaluronate solution, incubate at 37°C for 45 min, add 160 μL of acidic BSA solution, and let stand at room temperature for 10 min. Measure the absorbance of the reaction mixture at 600 nm using a microplate reader. The sample control group used an equal volume of PBS solution instead of hyaluronidase solution, the negative control group used an equal volume of PBS solution instead of sample solution, and the negative control group used an equal volume of PBS solution instead of both sample solution and hyaluronidase solution. 0.1 mg / mL sodium cromoglycate was used as a positive control. Calculate the hyaluronidase inhibition rate using the following formula:

[0211]

[0212] A1 - Absorbance of the negative control group (PBS + hyaluronidase + sodium hyaluronate + acidic BSA); A2 - Absorbance of the negative control group (PBS + sodium hyaluronate + acidic BSA); B1 - Absorbance of the sample group (sample + hyaluronidase + sodium hyaluronate + acidic BSA); B2 - Absorbance of the sample control group (sample + PBS + sodium hyaluronate + acidic BSA).

[0213] The experimental results of the effect of cosmetic composition on hyaluronidase inhibition rate are shown in Table 4.

[0214]

[0215] Analysis of experimental results:

[0216] (1) As shown in Table 4, the hyaluronidase inhibition rate of the cosmetic compositions prepared in Examples 1-4 is 64.70%~84.63%, which shows that they have significant inhibition of hyaluronidase activity.

[0217] (2) The hyaluronidase inhibition rates of Comparative Examples 1, 2, and 3 were 40.64%, 50.87%, and 30.22%, respectively. The results indicate that the combination of *Anoectochilus roxburghii* extract and *Corydalis yanhusuo* root extract in different proportions produced a clear synergistic effect. Their hyaluronidase inhibition rates were significantly higher than the additive effect of the single active ingredients, and the inhibitory effect increased in a dose-dependent manner with increasing *Anoectochilus roxburghii* extract concentration. These results confirm that the combination of *Anoectochilus roxburghii* extract and *Corydalis yanhusuo* root extract is the core factor determining the high efficiency of this composition in inhibiting hyaluronidase activity. Therefore, the cosmetic composition provided by this invention can effectively inhibit hyaluronidase activity, helping to reduce the degradation of hyaluronic acid in the skin, thereby maintaining skin moisture and elasticity, and exerting anti-inflammatory and soothing effects.

[0218] Comparative Examples 4-7: Investigation of Emulsifiers

[0219] Comparative Example 4: The only difference from Example 1 is that the polydimethylsiloxane and polydimethylsiloxane PEG-10 / 15 crosslinking polymers are replaced with cyclopentasiloxane (and) dimethylsiloxane crosslinking polymers, and the other conditions are the same as in Example 1.

[0220] Comparative Example 5: The only difference from Example 1 is that the polydimethylsiloxane and polydimethylsiloxane PEG-10 / 15 crosslinking polymers were replaced with Caprylyl Methicone (and) PEG-12 polydimethylsiloxane / PPG-20 crosslinking polymers, and the other conditions were the same as in Example 1.

[0221] Comparative Example 6: The only difference from Example 1 is that lauryl PEG-9 polydimethylsiloxane ethyl polydimethylsiloxane is replaced with PEG-11 methyl ether polydimethylsiloxane, and the other conditions are the same as in Example 1.

[0222] Comparative Example 7: The only difference from Example 1 is that lauryl PEG-9 polydimethylsiloxane ethyl polydimethylsiloxane is replaced with lauryl PEG-10 tris(trimethoxy)silyl ethyl polydimethylsiloxane, and the other conditions are the same as in Example 1.

[0223] Comparative Examples 8-9: Investigation of Thickeners

[0224] Comparative Example 8: The only difference from Example 1 is that distearate dimethylammonium lithium montmorillonite is replaced with cellulose gum, and the other conditions are the same as in Example 1.

[0225] Comparative Example 9: The only difference from Example 1 is that distearate dimethylammonium lithium montmorillonite is replaced with dimethylsilyl alkylated silica, and the other conditions are the same as in Example 1.

[0226] Test Example 7: Stability Test

[0227] The cosmetic compositions obtained in Examples 1 to 4 and Comparative Examples 4 to 9 were placed at 45°C for 3 months, and their appearance was tested. The results are shown in Table 5.

[0228]

[0229] in conclusion:

[0230] (1) As shown in Table 5, the cosmetic compositions of Examples 1-4 and Comparative Examples 4-9 all exhibited stable and uniform properties in the initial state (day 0).

[0231] (2) After being stored at 45°C for 3 months, the cosmetic compositions of Examples 1-4 remained stable and homogeneous without oil-water separation; while Comparative Examples 4-9 all showed oil-water separation or poor thickening effect.

[0232] (3) Comparative Examples 4-7 were investigated by replacing different types of emulsifiers. The results showed that the selected polydimethylsiloxane and polydimethylsiloxane PEG-10 / 15 cross-linked polymer and lauryl PEG-9 polydimethylsiloxane ethyl polydimethylsiloxane played a key role in maintaining emulsion stability in this system. Comparative Examples 8-9 were investigated by replacing thickeners. The results showed that distearate dimethylammonium lithium montmorillonite had better thickening and stabilizing effects in this water-in-oil system. The results prove that the specific combination of emulsifier and thickener selected in this invention is an important factor in achieving long-term product stability.

[0233] The method of this invention has been described through preferred embodiments. Those skilled in the art will readily be able to modify or appropriately alter and combine the methods and applications described herein within the scope, spirit, and context of this invention to implement and apply the technology of this invention. Those skilled in the art can refer to the content herein to appropriately improve process parameters. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art and are considered to be included within the scope of this invention.

Claims

1. A cosmetic composition, characterized in that, include: The active ingredients include *Anoectochilus roxburghii* extract and *Corydalis yanhusuo* root extract; the *Anoectochilus roxburghii* extract is a water-based extract, and the *Corydalis yanhusuo* root extract is an ethanol extract; the weight ratio of the *Anoectochilus roxburghii* extract to the *Corydalis yanhusuo* root extract is 0.6:1 to 1:1; the cosmetic composition is a water-in-oil formulation, and the cosmetic composition is prepared from components A, B, C, and D; wherein, based on the total weight of the cosmetic composition, component A includes: a first moisturizer 4.50wt% to 5.50wt%, sodium chloride 0.70wt% to 0.90wt%, 1,3-butanediol 9.00wt% to 11.00wt%, and trehalose 0.40wt% to 0. 0.55 wt% and water 52.00 wt%~55.00 wt%; the first moisturizer is composed of glycerin and sodium hyaluronate; based on the total weight of the cosmetic composition, component B includes: 0.40 wt%~0.55 wt% p-hydroxyacetophenone, 1.00 wt%~3.00 wt% 1,3-butanediol, 0.02 wt%~0.10 wt% β-glucan, 0.20 wt%~0.50 wt% tetrahydromethylpyrimidine carboxylic acid, 2.00 wt%~3.00 wt% anti-allergy agent, 0.40 wt%~0.70 wt% 1,2-hexanediol and 4.00 wt%~6.00 wt% water; based on the total weight of the cosmetic composition, component C... The components include: 3.00wt%~4.00wt% polydimethylsiloxane, 0.10wt%~0.30wt% polydimethylsiloxane PEG-10 / 15 crosspolymer, 1.00wt%~2.00wt% cyclopentasiloxane, 0.30wt%~0.45wt% cyclohexylsiloxane, 2.00wt%~3.60wt% caprylic / capric triglyceride, and 0.30wt%~0.60wt% thickener; the thickener includes distearate dimethylammonium lithium montmorillonite; based on the total weight of the cosmetic composition, component D includes: 2.00wt%~4.00wt% first emulsifier and 3.00wt%~5.00wt% cyclopentasiloxane. The first emulsifier comprises PEG-10 polydimethylsiloxane and lauryl PEG-9 polydimethylsiloxane ethyl polydimethylsiloxane; wherein the anti-sensitivity agent is composed of CTR50 and Anoectochilus roxburghii extract; based on the total weight of CTR50, CTR50 is composed of the following weight percentages: Corydalis yanhusuo root extract 4.00wt%~6.00wt%, 1,3-butanediol 78.00wt%~85.00wt%, citric acid 2.50wt%~4.00wt%, and isosorbide dimethyl ether 8.00wt%~12.00wt%.The total weight of the *Anoectochilus roxburghii* extract is calculated as follows: 2.00 wt% to 4.00 wt% of *Anoectochilus roxburghii* extract, 0.40 wt% to 0.60 wt% of p-hydroxyacetophenone, 8.00 wt% to 12.00 wt% of 1,3-butanediol, 0.80 wt% to 1.50 wt% of 1,2-hexanediol, 0.05 wt% to 0.20 wt% of ethylhexylglycerin, and 83.00 wt% to 87.00 wt% of water.

2. The cosmetic composition according to claim 1, wherein the method for preparing the *Anoectochilus roxburghii* extract comprises: The dried whole plant of *Anoectochilus roxburghii* was pulverized to obtain *Anoectochilus roxburghii* powder; Then, the *Anoectochilus roxburghii* powder and water are mixed, centrifuged, the supernatant is collected, concentrated, and freeze-dried to obtain the *Anoectochilus roxburghii* extract; the preparation method of the *Corydalis yanhusuo* root extract includes: soaking crude *Corydalis yanhusuo* root powder in ethanol and then refluxing to extract, evaporating and concentrating the extract until it becomes viscous, and then vacuum drying to obtain the *Corydalis yanhusuo* root extract.

3. The cosmetic composition according to claim 1, wherein, based on the total weight of the cosmetic composition, the content of glycerin in the first moisturizer is 4.48wt%~5.45wt%, and the content of sodium hyaluronate in the first moisturizer is 0.02wt%~0.05wt%; and based on the total weight of the cosmetic composition, the content of PEG-10 polydimethylsiloxane is 1.30wt%~2.50wt%, and the content of lauryl PEG-9 polydimethylsiloxane ethyl polydimethylsiloxane is 0.70wt%~1.50wt%.

4. The cosmetic composition according to claim 1, wherein the method for preparing the CTR50 comprises: S1: Mix 1,3-butanediol with 2 to 3 times the weight of citric acid, heat to 50 to 60°C to dissolve, and obtain mixture 1; S2: Take the remaining 1,3-butanediol, add Corydalis yanhusuo root extract, heat to 110~120℃, stir to dissolve, and obtain mixture 2; S3: When the temperature of mixture 2 obtained in step S2 drops to 90~95℃, add mixture 1 obtained in step S1 to mixture 2, stir evenly, and obtain mixture 3; S4: Add isosorbide dimethyl ether to the cooled mixture 3, mix evenly, and homogenize by high pressure microfluidic jet to obtain CTR50; The preparation method of the Anoectochilus roxburghii extract includes: (i) mixing p-hydroxyacetophenone with 1,3-butanediol, heating to 65~75℃ and dispersing evenly to obtain mixture 4; (ii) Cool the temperature of the mixture 4 obtained in step (i) to 45~65℃, add ethylhexylglycerin that has been dispersed in 1,2-hexanediol, stir evenly to obtain mixture 5; (iii) When the temperature of the mixture 5 obtained in step (ii) cools to 25℃~45℃, add water and Anoectochilus roxburghii extract, mix evenly to obtain Anoectochilus roxburghii extract.

5. A method for preparing the cosmetic composition according to any one of claims 1 to 4, characterized in that, The preparation method of the cosmetic composition includes: (1) mixing the components in component A and heating to 85~90℃ to dissolve them to obtain a first mixture; (2) lowering the temperature of the first mixture to 45~65℃, adding a 1,3-butanediol solution of p-hydroxyacetophenone, then adding an aqueous solution of β-glucan and tetrahydromethylpyrimidine carboxylic acid, and stirring; cooling to 45℃ or below, then mixing with other components in component B, and stirring to obtain a second mixture; (3) mixing polydimethylsiloxane PEG-10 / 15 crosspolymer, cyclopentadimethylsiloxane, and cyclohexylsiloxane in component C evenly, then adding a thickener that has been homogenized and dispersed with caprylic / capric triglyceride and polydimethylsiloxane, stirring evenly, then adding the components in component D, and stirring to obtain an oily mixture; (4) under stirring conditions of 100rpm~200rpm, slowly adding the second mixture to the oily mixture, and after the addition is completed, increasing the speed to 400rpm. Stir at 100 rpm to 500 rpm for 5 to 10 minutes to initially mix the system evenly, and then homogenize at 2000 rpm to 3000 rpm for 2 to 5 minutes to obtain the cosmetic composition.

6. The use of a cosmetic composition according to any one of claims 1 to 4 or a cosmetic composition prepared by the preparation method according to claim 5 in the preparation of cosmetics for skin soothing and / or repair.

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