Botanical insecticide for preventing and treating fall webworms of forest trees and preparation method of botanical insecticide
By loading matrine onto chitosan-guar gum-functionalized silica nanomaterials, the problems of stability and slow action of plant-derived pesticides in controlling the fall webworm in forests have been solved, achieving a more efficient insecticidal effect, which is suitable for organic agriculture and green food production.
Patent Information
- Application Number
- CN202510456851.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-12
- Publication Date
- 2026-02-17
AI Technical Summary
Existing plant-derived pesticides, such as matrine, have problems with slow action and easy degradation when controlling the fall webworm in forests, which affects their control efficacy.
Matrine is loaded onto functionalized nano-silica, especially through chitosan-guar gum-functionalized silica-loaded matrine nanomaterials, to form chitosan-guar gum-functionalized silica-loaded matrine nanomaterials, which enhances the stability and insecticidal effect of pesticides.
It improves the insecticidal effect of matrine on the fall webworm, prevents premature pesticide release, enhances delivery efficiency and stability, and meets the standards of organic agriculture and green food production.
Smart Images
Figure BDA0005355766510000051 
Figure HDA0005355766520000011
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of forestry prevention and control, and particularly relates to a plant source insecticide for preventing and treating forest American white moth and a preparation method thereof. BACKGROUND
[0002] American white moth (scientific name: Hyphantria cunea) is a global invasive species originally from North America, belonging to Lepidoptera Arctiidae, and having strong reproductive capacity and adaptability. The adult body is about 15 mm long, the wing span is 25-35 mm, the whole body is white, and some individuals have black spots on the forewings. The larva body can be up to 35 mm long, and the body surface is densely covered with black nodules and white long hairs. When the group moves, it will spit silk to form a significant nest. The species has 2-3 generations a year, and a single female can lay up to 800 eggs. The larvae feed on more than 300 plant species, especially mulberry trees, fruit trees and street trees, which can cause trees to shed leaves and even die. Since it was first introduced into Europe in 1940, it has spread to many countries in Asia and South America.
[0003] Matrine is a natural quinoline alkaloid extracted from Sophora flavescens and Sophora alopecuroides and other plants, which is a typical representative of plant source pesticides. It has multiple biological activities such as insecticidal, antibacterial, antiviral and plant growth regulation. In the field of agriculture, matrine is widely used in green pest control. Its main mechanism of action is to interfere with the acetylcholine receptors of the nervous system of pests, disrupt their nerve signal transmission, and cause paralysis and death. At the same time, it can inhibit the enzyme activity or mycelial growth of pathogenic bacteria, and reduce the occurrence of diseases. Compared with chemical pesticides, matrine has high contact and stomach toxicity on common pests such as Lepidoptera (such as cabbage worms and cotton bollworms), aphids and red spiders, and is safe to bees and natural enemy insects, has low residue and is easy to degrade, and meets the standards of organic agriculture and green food production.
[0004] Plant source pesticides are concerned due to their low toxicity and high biological safety, but they have defects such as slow action and easy degradation, and it is urgent to develop environmentally friendly means to improve their activity and stability. SUMMARY
[0005] To solve the problems in the prior art, the present application provides a plant source insecticide for preventing and treating forest American white moth and a preparation method thereof.
[0006] The present application is realized by the following technical scheme:
[0007] A plant source insecticide for preventing and treating forest American white moth, wherein the plant source insecticide is matrine loaded on functionalized nano-silica to obtain a functionalized nano-silica loaded matrine material.
[0008] Further, the functionalized silica loaded matrine nanomaterial is a chitosan functionalized silica loaded matrine nanomaterial.
[0009] Further, the preparation method of the chitosan functionalized silica loaded matrine nanomaterial is as follows:
[0010] 600 mg of chitosan and 150 mg of guar gum are added to 100 mL of 0.5% (V / V) acetic acid water, the pH is adjusted to 6 using 2M sodium hydroxide, and then ultrasonic treatment is performed for 30 min, followed by stirring at 500 rpm for 12 h to obtain a chitosan solution;
[0011] 100 mg of nanosilica is dispersed in 5 mL of a 20 g / L matrine aqueous solution, vortexed to mix, and then ultrasonic treatment is performed for 30 min; then 20 mL of the chitosan solution is added, vortexed to mix, and then ultrasonic treatment is performed for 1 h; stirring is performed at room temperature of 25℃ and at a speed of 600 rpm for 48 h, and then centrifugation is performed to obtain a precipitate, which is freeze-dried to obtain the chitosan functionalized silica loaded matrine nanomaterial.
[0012] Further, the synthesis method of the nanosilica is as follows: 2.96 g of CTAB is dissolved in 100 mL of deionized water, and the pH is adjusted to 11 using NaOH; then heating is performed to 80℃, 1.86 mL of TEOS is added, stirring is performed for 30 min, and then cooling is performed to 30℃; the solution is stirred at 30℃ and at a speed of 550 rpm for 24 h; then the solution is heated to 80℃, and maintained for 24 h; centrifugation is performed to obtain a precipitate, which is washed with ethanol for 3 times, the precipitate is vacuum dried at room temperature for 24 h, and then the dried product is heated to 600℃ in a graphite furnace and maintained at this temperature for 5 h to obtain the nanosilica.
[0013] Further, the functionalized silica loaded matrine nanomaterial is a chitosan functionalized silica loaded matrine nanomaterial.
[0014] Further, the preparation method of the chitosan functionalized silica loaded matrine nanomaterial is as follows:
[0015] 600 mg of chitosan is added to 100 mL of 0.5% (V / V) acetic acid water, the pH is adjusted to 6 using 2M sodium hydroxide, and then ultrasonic treatment is performed for 30 min, followed by stirring at 500 rpm for 12 h to obtain a chitosan solution;
[0016] 100mg nano-silica was dispersed into 5mL 20g / L matrine aqueous solution, vortex mixed, and then ultrasonic for 30min; then 20mL chitosan solution was added, vortex mixed, and then ultrasonic for 1h; stirring at 600rpm for 48h at room temperature 25℃, and then centrifugation to obtain the precipitate, and the precipitate was freeze-dried to obtain the nano-silica loaded with matrine.
[0017] Further, the present application also provides a preparation method of the plant source insecticide for preventing and treating forest American white moth, and the insecticide is dispersed in water, wherein the concentration of the insecticide is 5-10mg / L.
[0018] Compared with the prior art, the present application has the following advantages and beneficial effects:
[0019] The silica nanoparticles have the characteristics of surface modifiability, large specific surface area, large pore volume and strong stability, but the exposed pores are easy to cause the premature release of the pesticide, thereby reducing the delivery efficiency, but when chitosan and guar gum are used as the encapsulation material, the premature release of the pesticide can be prevented. Meanwhile, the chitosan guar gum functionalized silica loaded matrine nanomaterial has a more excellent insecticidal effect on the American white moth than the chitosan functionalized silica alone. BRIEF DESCRIPTION OF DRAWINGS
[0020] Figure 1 It is an SEM image of the chitosan guar gum functionalized silica loaded matrine nanomaterial of the present application embodiment 1. DETAILED DESCRIPTION
[0021] In order to make the purpose, technical scheme and advantages of the present application more clear, specific embodiments of the present application are described in detail below, and the schematic embodiments of the present application and the description thereof are only used to explain the present application, and not as a limitation of the present application.
[0022] In the present application, CTAB is cetyltrimethylammonium bromide, TEOS is tetraethoxysilane, the molecular weight of chitosan is 310000-375000Da, and all reagents are commercially available products without further treatment.
[0023] Embodiment 1
[0024] A plant source insecticide for preventing and treating forest American white moth, wherein the insecticide is obtained by loading the plant source insecticide matrine on nano-silica to obtain a chitosan guar gum functionalized silica loaded matrine nanomaterial, Figure 1 It is an SEM image of the chitosan guar gum functionalized silica loaded matrine nanomaterial of the present application embodiment 1.
[0025] The preparation method of the chitosan guar gum functionalized silica loaded matrine nanomaterial is as follows:
[0026] 600 mg chitosan and 150 mg guar gum were added to 100 mL 0.5% (V / V) acetic acid water, pH was adjusted to 6 using 2M sodium hydroxide, then ultrasonic for 30 min, then stirred at 500 rpm for 12 h to obtain chitosan solution;
[0027] 100 mg nano-silica was dispersed into 5 mL 20 g / L matrine aqueous solution, vortex mixed, then ultrasonic for 30 min; then 20 mL chitosan solution was added, vortex mixed, then ultrasonic for 1 h; stirred at 600 rpm for 48 h at room temperature 25℃, then centrifuged to obtain precipitate, the precipitate was freeze-dried to obtain chitosan guar gum functionalized silica loaded matrine nanomaterial.
[0028] Wherein the synthesis of nano-silica: 2.96 g CTAB was dissolved in 100 mL deionized water, pH was adjusted to 11 using NaOH; then heated to 80℃, 1.86 mL TEOS was added, stirred for 30 min, then cooled to 30℃; the solution was stirred at 550 rpm for 24 h at 30℃; then the solution was heated to 80℃ and maintained for 24 h; then centrifuged to obtain precipitate, washed with ethanol for 3 times, the precipitate was vacuum dried at room temperature for 24 h, then the dried product was heated to 600℃ in a graphite furnace and maintained at this temperature for 5 h to obtain nano-silica.
[0029] Example 2
[0030] A plant-derived insecticide for preventing and treating forest American white moth, the insecticide is loading plant-derived insecticide matrine on nano-silica to obtain chitosan functionalized silica loaded matrine nanomaterial.
[0031] The preparation method of the chitosan functionalized silica loaded matrine nanomaterial is different from that of example 1 in that it does not contain guar gum, specifically: 600 mg chitosan was added to 100 mL 0.5% (V / V) acetic acid water, pH was adjusted to 6 using 2M sodium hydroxide, then ultrasonic for 30 min, then stirred at 500 rpm for 12 h to obtain chitosan solution;
[0032] 100 mg nano-silica was dispersed into 5 mL 20 g / L matrine aqueous solution, vortex mixed, then ultrasonic for 30 min; then 20 mL chitosan solution was added, vortex mixed, then ultrasonic for 1 h; stirred at 600 rpm for 48 h at room temperature 25℃, then centrifuged to obtain precipitate, the precipitate was freeze-dried to obtain nano-silica loaded matrine.
[0033] Wherein the synthesis of nanosilica: take CTAB 2.96g dissolved in 100ml deionized water, using NaOH to adjust the pH to 11; then heated to 80℃, add 1.86ml TEOS, stirring for 30min, then cooled to 30℃; the solution at 30℃, stirring at 550rpm for 24h; then heated to 80℃, maintain 24h; then centrifugation to obtain the precipitate, washed with ethanol 3 times, precipitate in vacuum drying at room temperature for 24h, then dry product heated to 600℃ in a graphite furnace and maintain the temperature for 5h, to obtain nanosilica.
[0034] Test Example 1
[0035] The forest test site is a poplar seedling base with good soil fertility, soil pH is 7.4, and the poplar grows well. No other pesticides are applied during the experiment.
[0036] The pesticide is diluted according to the proportion and sprayed evenly on the whole plant.
[0037] The pesticides in the examples and comparative examples are tested according to the proportion in the table, and 4 poplar trees are randomly selected as a block. At least 10 meters are set between each block. Each poplar tree in each block is marked with 5 insect-infested branches in the southeast, southwest, northwest, and center. The number of insect pests is investigated. The number of insect pests is measured before application, 3 days after application, 7 days after application, and 14 days after application, and the insect pest reduction rate is calculated. The insect pest reduction rate = (the number of live insects before application - the number of live insects after application) / the number of live insects before application x 100%.
[0038] The loaded matrine nanosilica obtained in Example 1 and Example 2 is configured into a water solution with a concentration of 5mg / L, 7mg / L, and 10mg / L for testing. Pure matrine water solution is used for comparison, with concentrations of 5mg / L, 7mg / L, and 10mg / L.
[0039] Table 1 Performance test.
[0040]
[0041] As can be seen from Table 1, the silica nanoparticles in Example 1 and Example 2 have the characteristics of surface modifiability, large specific surface area, large pore volume, and strong stability, but the exposed pores can easily cause premature release of pesticides, reducing the delivery efficiency. However, when chitosan and guar gum are used as encapsulation materials, premature release of pesticides can be prevented. At the same time, the chitosan guar gum functionalized silica loaded matrine nanomaterial has a more excellent insecticidal effect on Hyphantria cunea than chitosan alone.
[0042] The above detailed description of the specific embodiments of the present application has been given to understand the purpose, technical solutions and beneficial effects of the present application. It should be understood that the above description is only a specific embodiment of the present application and is not used to limit the protection scope of the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims
1. A plant-derived insecticide for controlling the fall webworm in forest trees, characterized in that, The plant source insecticide is to load the plant source insecticide matrine on the functionalized nano-silica to obtain the functionalized nano-silica loaded matrine material.
2. The plant source insecticide for preventing and treating forest American white moth according to claim 1, wherein the functionalized nano-silica loaded matrine material is a chitosan guar gum functionalized nano-silica loaded matrine material.
3. The plant source insecticide for preventing and treating forest American white moth according to claim 2, wherein the preparation method of the chitosan guar gum functionalized nano-silica loaded matrine material is as follows: 600 mg of chitosan and 150 mg of guar gum are added into 100 mL of 0.5% (V / V) acetic acid water, 2M sodium hydroxide is used to adjust the pH to 6, then ultrasonic treatment is performed for 30 min, then stirring is performed at 500 rpm for 12 h to obtain a chitosan solution; 100 mg of nano-silica is dispersed into 5 mL of 20 g / L matrine aqueous solution, vortex mixing is performed, then ultrasonic treatment is performed for 30 min; then 20 mL of the chitosan solution is added, vortex mixing is performed, then ultrasonic treatment is performed for 1 h; stirring is performed at 600 rpm at room temperature of 25℃ for 48 h, then centrifugation is performed to obtain a precipitate, the precipitate is freeze-dried to obtain the chitosan guar gum functionalized nano-silica loaded matrine material.
4. The plant source insecticide for preventing and treating forest American white moth according to claim 3, wherein the synthesis method of the nano-silica is as follows: 2.96 g of CTAB is dissolved in 100 mL of deionized water, NaOH is used to adjust the pH to 11; then heating is performed to 80℃, 1.86 mL of TEOS is added, stirring is performed for 30 min, then cooling is performed to 30℃; the solution is stirred at 550 rpm at 30℃ for 24 h; then the solution is heated to 80℃, and maintained for 24 h; then centrifugation is performed to obtain a precipitate, the precipitate is washed with ethanol for 3 times, the precipitate is vacuum dried at room temperature for 24 h, then the dried product is heated to 600℃ in a graphite furnace and maintained at this temperature for 5 h to obtain the nano-silica.
5. The plant source insecticide for preventing and treating forest American white moth according to claim 1, wherein the functionalized nano-silica loaded matrine material is a chitosan functionalized nano-silica loaded matrine material. The preparation method of the chitosan functionalized nano-silica loaded matrine material is as follows: 600 mg of chitosan is added into 100 mL of 0.5% (V / V) acetic acid water, 2M sodium hydroxide is used to adjust the pH to 6, then ultrasonic treatment is performed for 30 min, then stirring is performed at 500 rpm for 12 h to obtain a chitosan solution; 100 mg of nano-silica is dispersed into 5 mL of 20 g / L matrine aqueous solution, vortex mixing is performed, then ultrasonic treatment is performed for 30 min; then 20 mL of the chitosan solution is added, vortex mixing is performed, then ultrasonic treatment is performed for 1 h; stirring is performed at 600 rpm at room temperature of 25℃ for 48 h, then centrifugation is performed to obtain a precipitate, the precipitate is freeze-dried to obtain the nano-silica loaded matrine.
6. The plant source insecticide for preventing and treating forest American white moth according to claim 5, wherein the synthesis method of the nano-silica is as follows: 2.96 g of CTAB is dissolved in 100 mL of deionized water, NaOH is used to adjust the pH to 11; then heating is performed to 80℃, 1.86 mL of TEOS is added, stirring is performed for 30 min, then cooling is performed to 30℃; the solution is stirred at 550 rpm at 30℃ for 24 h; then the solution is heated to 80℃, and maintained for 24 h; then centrifugation is performed to obtain a precipitate, the precipitate is washed with ethanol for 3 times, the precipitate is vacuum dried at room temperature for 24 h, then the dried product is heated to 600℃ in a graphite furnace and maintained at this temperature for 5 h to obtain the nano-silica.
6. The botanical insecticide for controlling forest tree Malacosoma neustria according to claim 5, characterized in that, 7. The botanical insecticide for controlling forest tree Malacosoma neustria according to claim 6, characterized in that, The method for synthesizing the nanosilica is as follows: 2.96 g of CTAB is dissolved in 100 mL of deionized water, and the pH is adjusted to 11 using NaOH; then heated to 80 DEG C, 1.86 mL of TEOS is added, and the reaction is stirred for 30 min, and then cooled to 30 DEG C; the solution is stirred at 550 rpm for 24 h at 30 DEG C; then the solution is heated to 80 DEG C, and maintained for 24 h; then centrifuged to obtain a precipitate, which is washed with ethanol for 3 times, and the precipitate is dried in vacuum at room temperature for 24 h, then the dried product is heated to 600 DEG C in a graphite furnace and maintained at this temperature for 5 h, to obtain nanosilica.
8. The plant-derived insecticide for controlling the fall webworm in forest trees according to any one of claims 1 to 7, characterized in that, The forest tree is poplar.
9. The method of claim 1 to 7, wherein the method is characterized by, The insecticide is dispersed in water, wherein the concentration of the insecticide is 5-10 mg / L.