Hyphantria cunea attractant and application thereof

Abstract

The invention provides a fall webworm attractant and application thereof, and belongs to the technical field of attractants. The hyphantria cunea attractant comprises sex pheromone and an odor compound, the sex pheromone is composed of (3Z, 6Z, 9S, 10R)-9, 10-epoxy-3, 6-heneicosadiene, (3Z, 6Z, 9S, 10R)-9, 10-epoxy-1, 3, 6-heneicosatriene and (9Z, 12Z, 15Z)-octadecatrienal, and the sex pheromone is composed of (3Z, 6Z, 9S, 10R)-9, 10-epoxy-3, 6-heneicosadiene, (3Z, 6Z, 9S, 10R)-9, 10-epoxy-1, 3, 6-heneicosatriene and ( And the odor compound is dodecane or 1, 4-diethylbenzene. According to the sex pheromone, the odor compound is added into the sex pheromone, so that the sex pheromone has a good attraction effect on fall webworms, the trapping effect is improved, and the attraction effect of the sex pheromone on the fall webworms is improved.

Description

Technical Field

[0001] This invention belongs to the field of attractant technology, and particularly relates to an attractant for the American white moth and its application. Background Technology

[0002] American white moth ( Hyphantria cunea The fall webworm (Dury) is a global quarantine pest. Due to its wide host range, large feeding capacity, high reproductive rate, and rapid spread, it is listed in the national list of dangerous forest pests. It damages more than 300 species of plants in 49 families, including trees, fruit trees, flowers, and crops. The larvae feed on the leaves of host plants, weakening the trees. In large-scale outbreaks, the leaves are often completely defoliated, severely impacting tree growth and even causing tree death, posing a serious threat to forest resources and ecological security. Monitoring the fall webworm is essential for controlling its spread and damage. Sex pheromones, with their high specificity and accurate monitoring, have become a widely used monitoring method in production units.

[0003] Sex pheromones are trace chemical substances used in sexual communication among lepidopteran insects. They are generally released by the gonads of female moths and attract male moths from a distance, exhibiting species specificity. Sex pheromones are attractants based on sex pheromones, used for large-scale trapping and population monitoring of target insect male moths, and are widely used for the control of most agricultural and forestry pests. Utilizing sex pheromones for monitoring and early warning of the fall webworm, and understanding its occurrence dynamics, is a prerequisite for timely control. Summary of the Invention

[0004] In view of this, one of the objectives of the present invention is to provide an attractant for the American white moth.

[0005] The second objective of this invention is to provide a method for attracting the American white moth.

[0006] A third objective of this invention is to provide the application of the American white moth attractant or the method in attracting American white moths.

[0007] To achieve the above-mentioned objectives, the present invention provides the following technical solution: A fall webworm attractant comprising a sex pheromone and an odor compound; the sex pheromone being composed of (3Z, 6Z, 9S, 10R)-9,10-epoxy-3,6-docosadiene, (3Z, 6Z, 9S, 10R)-9,10-epoxy-1,3,6-docosatriene and (9Z, 12Z, 15Z)-octadecanetrienal; the odor compound being dodecane or 1,4-diethylbenzene.

[0008] Preferably, the mass ratio of (3Z, 6Z, 9S, 10R)-9,10-epoxy-3,6-tetracarbodiene, (3Z, 6Z, 9S,10R)-9,10-epoxy-1,3,6-tetracarbotriene and (9Z, 12Z, 15Z)-octadecanetrienal is 2:0.6:1.

[0009] Preferably, the mass ratio of odorant compound to sex pheromone in the American white moth attractant is 0.1 to 10:1.

[0010] Preferably, the fall webworm attractant also includes an organic solvent.

[0011] Preferably, the organic solvent is dichloromethane.

[0012] The present invention also provides a method for attracting the fall webworm, comprising the following steps: Add the attractant for the American white moth to a carrier to make a lure, place the lure in a trap, and hang the trap in the forest.

[0013] Preferably, the trap is suspended at a height of ≥2m.

[0014] Preferably, the distance between two adjacent traps is 40~60m.

[0015] The present invention also provides the application of the said fall webworm attractant or the said method in attracting fall webworms.

[0016] Preferably, the fall webworm attractant is used to attract adult fall webworms.

[0017] Compared with the prior art, the present invention has the following beneficial effects: This invention provides an attractant for the fall webworm. By adding odorous compounds to the sex pheromones, this invention has a good attraction effect on the fall webworm, increases the trapping effect, and enhances the attraction of sex pheromones to male adult fall webworms. Attached Figure Description

[0018] Figure 1 The figures show the EAG response of male adult American white moths to mixtures of odor compounds and sex pheromones in different proportions. In each figure, A represents an odor compound concentration of 1 mg / mL, B represents an odor compound concentration of 10 mg / mL, and C represents an odor compound concentration of 100 mg / mL. express P <0.05; Figure 2The Y-tube olfactory behavior of male adult American white moths in response to different attractants is shown. Dodecane is the attractant in Example 1, 2-ethylhexanol is the attractant in Comparative Example 3, 1,4-diethylbenzene is the attractant in Example 7, and m-xylene is the attractant in Comparative Example 5. express P <0.05, express P <0.01, ns indicates no significant difference; Figure 3 This is a side view of the wind tunnel setup; Figure 4 The wind tunnel behavior of unmated male adult American white moths in response to different attractants was shown. The standard group served as the control group, dodecane was the attractant in Example 2, 1,4-diethylbenzene was the attractant in Example 8, 2-ethylhexanol was the attractant in Comparative Example 4, and m-xylene was the attractant in Comparative Example 8. express P <0.05; Figure 5 To investigate the use of different attractants for the field trapping of male adult American white moths, the standard group served as the control group, A was the attractant in Comparative Example 8, B was the attractant in Comparative Example 4, C was the attractant in Example 2, and D was the attractant in Example 8. express P <0.05. Detailed Implementation

[0019] This invention provides an attractant for the fall webworm, comprising a sex pheromone and an odor compound. The sex pheromone is composed of (3Z, 6Z, 9S, 10R)-9,10-epoxy-3,6-cuicocarbodiene, (3Z, 6Z, 9S, 10R)-9,10-epoxy-1,3,6-cuicocarbotriene, and (9Z, 12Z, 15Z)-octadecanetrienal. The odor compound is dodecane or 1,4-diethylbenzene. Different insect species exhibit interspecific olfactory perception; the same volatile substance often produces different physiological or behavioral effects on different insect species. The odor compound contained in the attractant of this invention can effectively attract the fall webworm.

[0020] In this invention, the preferred mass ratio of (3Z, 6Z, 9S, 10R)-9,10-epoxy-3,6-tetracarbodiene (C21-2Epo), (3Z, 6Z, 9S, 10R)-9,10-epoxy-1,3,6-tetracarbotriene (C21-3Epo) and (9Z,12Z, 15Z)-octadecanetrienal (C18: 3Ald) in the sex information is 2:0.6:1.

[0021] In this invention, the mass ratio of the odorant compound to the sex pheromone in the fall webworm attractant is 0.1 to 10:1, and in some examples, it is preferably 0.1:1, 1:1, or 10:1. The fall webworm attractant also includes an organic solvent, preferably dichloromethane. When the fall webworm attractant includes an organic solvent, the concentration of the sex pheromone is preferably 10 mg / mL. In this invention, the sex pheromone and the odorant compound work together to effectively enhance the attraction to male adult fall webworms.

[0022] This invention also provides a method for attracting the fall webworm, comprising the following steps: adding fall webworm attractant to a carrier to make a lure, placing the lure in a trap, and suspending the trap in the forest. The trap is preferably suspended at a height of ≥2m, and the distance between two adjacent traps is preferably 40~60m, more preferably 50m.

[0023] This invention also provides the application of the fall webworm attractant or the method described above in attracting fall webworms, wherein the fall webworm attractant is used to attract adult fall webworms, preferably male fall webworms. This invention can not only be used to disrupt mating and kill large numbers of male fall webworms, but also to effectively monitor fall webworm populations.

[0024] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0025] Example 1 A type of attractant for the American white moth: The sex pheromone is a mixture of (3Z, 6Z, 9S, 10R)-9,10-epoxy-3,6-tetracarbodiene, (3Z, 6Z, 9S, 10R)-9,10-epoxy-1,3,6-tetracarbotriene, and (9Z, 12Z, 15Z)-octadecanetrienal in a mass ratio of 2:0.6:1; the odor compound is dodecane; and the organic solvent is dichloromethane.

[0026] Dodecane was dissolved in dichloromethane to make the dodecane concentration 1 mg / mL, and then sex pheromones were added to make the sex pheromone concentration 10 mg / mL.

[0027] Example 2 A type of attractant for the American white moth: The sex pheromone is a mixture of (3Z, 6Z, 9S, 10R)-9,10-epoxy-3,6-tetradecadiene, (3Z, 6Z, 9S,10R)-9,10-epoxy-1,3,6-tetradecadiene, and (9Z, 12Z, 15Z)-octadectrienal in a mass ratio of 2:0.6:1; the odor compound is dodecane; the odor compound and the sex pheromone are mixed in a mass ratio of 0.1:1, without any organic solvent.

[0028] Example 3 An attractant for the American white moth, which differs from Example 1 in that: dodecane is dissolved in dichloromethane to achieve a dodecane concentration of 10 mg / mL.

[0029] Example 4 An attractant for the American white moth, which differs from Example 2 in that: the odor compound and sex pheromone are mixed in a 1:1 mass ratio.

[0030] Example 5 An attractant for the American white moth, which differs from Example 1 in that: dodecane is dissolved in dichloromethane to achieve a dodecane concentration of 100 mg / mL.

[0031] Example 6 An attractant for the American white moth, which differs from Example 2 in that: the odor compound and the sex pheromone are mixed at a mass ratio of 10:1.

[0032] Example 7 An attractant for the American white moth, which differs from Example 1 in that the odor compound is 1,4-diethylbenzene.

[0033] Example 8 An attractant for the American white moth, which differs from Example 2 in that the odor compound is 1,4-diethylbenzene.

[0034] Example 9 An attractant for the American white moth, which differs from Example 3 in that the odor compound is 1,4-diethylbenzene.

[0035] Example 10 An attractant for the American white moth, which differs from Example 4 in that the odor compound is 1,4-diethylbenzene.

[0036] Example 11 An attractant for the American white moth, which differs from Example 5 in that the odor compound is 1,4-diethylbenzene.

[0037] Example 12 An attractant for the American white moth, which differs from Example 6 in that the odor compound is 1,4-diethylbenzene.

[0038] Comparative Example 1 An attractant for the American white moth, which differs from Example 1 in that the odor compound is 2-ethylhexanol.

[0039] Comparative Example 2 An attractant for the American white moth, which differs from Example 3 in that the odor compound is 2-ethylhexanol.

[0040] Comparative Example 3 An attractant for the American white moth, which differs from Example 5 in that the odor compound is 2-ethylhexanol.

[0041] Comparative Example 4 An attractant for the American white moth, which differs from Example 6 in that the odor compound is 2-ethylhexanol.

[0042] Comparative Example 5 An attractant for the American white moth, which differs from Example 1 in that the odor compound is m-xylene.

[0043] Comparative Example 6 An attractant for the American white moth, which differs from Example 3 in that the odor compound is m-xylene.

[0044] Comparative Example 7 An attractant for the American white moth, which differs from Example 5 in that the odor compound is m-xylene.

[0045] Comparative Example 8 An attractant for the American white moth, which differs from Example 2 in that the odor compound is m-xylene.

[0046] Example 13 The activity of different attractants for the fall webworm.

[0047] Different attractants for the fall webworm were tested as follows.

[0048] 1. Antennae potential (EAG) measurement: First, using sterilized surgical scissors, the antennae of the male American white moth were cut off from the base. Under a stereomicroscope, the flagellum at the end of the antennal flagellum was removed. Then, the two ends of the antennae were fixed between Ag / AgCl electrodes containing physiological saline. The antennal potential signal was amplified by a Syntech IDA232 and connected to the main unit. The amplitude value of the antennal potential response was recorded using Auto Spike software. Using a pipette, 10 μL of the test solvent was evenly added to qualitative filter paper (0.45cm × 4cm). After standing for 5 seconds, the qualitative filter paper strip was held tightly against the inner wall of a Pasteur tube (7mm × 150mm) and placed inside. The end of the Pasteur tube was inserted into the small hole on the side of the EAG odor mixing tube. For each treatment, 6 one-day-old unmated male moths were selected, and one antenna of each moth was tested. Each antenna was stimulated 5 times consecutively. The flow rate of each stimulation was 60 mL / s, the stimulation time was 0.5 s, and the time interval between two stimulations was set to 60 s. The indoor ambient temperature for the test was 25±2 ℃.

[0049] The formula for calculating the relative response value of antennal potential is as follows: Relative response value of EAG = Average EAG response value of experimental group - Average EAG response value of control group.

[0050] The treatment groups consisted of Examples 1, 3, 5, 7, 9, 11, Comparative Examples 1, 2, 3, 5, 6, and 7. The control group consisted of sex pheromones dissolved in dichloromethane at a concentration of 10 mg / mL. The sex pheromones were a mixture of (3Z, 6Z, 9S, 10R)-9,10-epoxy-3,6-tetradecadiene, (3Z, 6Z, 9S, 10R)-9,10-epoxy-1,3,6-tetradecadiene, and (9Z, 12Z, 15Z)-octadectrienal in a mass ratio of 2:0.6:1.

[0051] Data processing and analysis: One-way ANOVA and subsequent Tukey method were used to analyze the EAG response of male adult American white moths to different ratios of odor compounds and sex pheromones (P<0.05). EAG experimental data were compiled and summarized using Excel, and statistical analysis was performed using SPSS 26.0 software. Charts were generated using Origin 2024.

[0052] The results are as follows Figure 1As shown, the EAG reaction values ​​of different odor compounds mixed with sex pheromones varied. Dodecane mixed with sex pheromones showed a higher EAG reaction value than the other three groups of odor compounds, while m-xylene mixed with sex pheromones showed the lowest EAG reaction value. Within the same odor compound, EAG reaction values ​​also varied at different working concentrations. Dodecane showed the highest reaction value at a concentration of 1 mg / mL in the attractant. As the concentration of the odor compound increased, the EAG reaction weakened, with 1,4-diethylbenzene and m-xylene showing the same trend as dodecane. However, 2-ethylhexanol showed the highest EAG reaction value at a concentration of 100 mg / mL in the attractant, showing the opposite trend to the other three odor compounds. Dodecane, 1,4-diethylbenzene, and m-xylene showed the strongest reaction activity in male insects at a mass ratio of 0.1:1 with sex pheromones. 2-ethylhexanol showed the strongest reaction activity in adult males at a mass ratio of 10:1 with sex pheromones.

[0053] 2. Y-type olfactory sensor behavioral response measurement: The solvent to be tested was the attractant from Examples 1, 7, 3, and 5.

[0054] The olfactory behavior test of the fall webworm was conducted under low-light conditions. The test time was from 4:00 AM to 5:30 AM Beijing time. The two sidewall ports of the Y-type olfactometer were connected sequentially to a scent source bottle, activated carbon, a gas flow meter, a distilled water bottle, and an atmospheric sampler. The gas flow rate was 500 mL / min. -1 The experiment used a 5cm diameter semi-circular filter paper as a reagent carrier. 10μL of the test solvent was added to the flavor source bottle of the treatment arm using a pipette, while 10μL of a 10mg / mL sex pheromone (a mixture of (3Z, 6Z, 9S, 10R)-9,10-epoxy-3,6-docosadiene, (3Z, 6Z, 9S, 10R)-9,10-epoxy-1,3,6-docosatriene and (9Z, 12Z, 15Z)-octadecanetrienal in a mass ratio of 2:0.6:1, soluble in dichloromethane) was added to the flavor source bottle of the control arm.

[0055] During the test, one male American white moth was placed at the opening of the main arm tube. After 5 minutes, once the moth had stopped moving, the position of each moth was observed and recorded. When an insect remained more than one-third of the way along either side of the tube wall, it was recorded as having selected the odor from the odor source bottle on that side. If the insect did not react within 5 minutes, it was recorded as not having selected the odor. Each odor was tested in three replicates, with 10 male moths tested in each group. After testing 5 adults each time, the positions of the test and control arms were switched to prevent uneven lighting from affecting the behavior of the male American white moths. A new odor source bottle and filter paper strip were used after testing 10 adults to avoid odor residue affecting the experimental results.

[0056] Response rate (%) = [(Number of worms in the control arm + Number of worms in the treatment arm) / Total number of worms] × 100%; Treatment selection rate (%) = [number of worms in the test arm / total number of worms] × 100%.

[0057] All data were analyzed using Excel and SPSS 26.0. Charts were created using Origin 2024.

[0058] The results are as follows Figure 2 As shown, when dodecane, 1,4-diethylbenzene, and sex pheromones were mixed at a mass ratio of 0.1:1, the selectivity rates of the treatment group were greater than those of the control group. However, when m-xylene and sex pheromones were mixed at a mass ratio of 0.1:1, the selectivity rate of the control group was greater than that of the treatment group, indicating that mixing m-xylene with sex pheromones reduces the olfactory perception of sex pheromones in the fall webworm. When 2-ethylhexanol and sex pheromones were mixed at a mass ratio of 10:1, the selectivity rate of the test group was also greater than that of the control group. The experimental results indicate that dodecane, 2-ethylhexanol, and 1,4-diethylbenzene all enhance the olfactory perception of sex pheromones in male adult fall webworms.

[0059] Y-type olfactometer experiments revealed that dodecane and 1,4-diethylbenzene, when mixed with sex pheromones at a mass ratio of 0.1:1, and 2-ethylhexanol, when mixed with sex pheromones at a mass ratio of 10:1, elicited a better olfactory response in American white moths than the single sex pheromone group.

[0060] 3. Wind tunnel behavioral responses of unmated male adult fall webworms to attractants: Take such Figure 3 The device shown was tested. The wind tunnel was divided into three different zones: a non-reactive zone at 0-0.4 m from the release point, a directional flight zone at 0.4 m-1.2 m, and a zone approaching the odor source at 12 m-1.5 m. The test was conducted under low-light conditions from 4:00 AM to 5:30 AM Beijing time. The wind speed was set to 0.3 m / s. The ambient temperature of the wind tunnel was set to 24±1℃, and the relative humidity was set to 60%±10%. Before the test, 15 μL of the attractants provided in Examples 2, 8, 4, and 8 were added to different carriers. These carriers were then suspended 0.15 m from the filter plate. A control was prepared by adding 15 μL of sex pheromones (a mixture of (3Z, 6Z, 9S, 10R)-9,10-epoxy-3,6-docosadiene, (3Z, 6Z, 9S, 10R)-9,10-epoxy-1,3,6-docosatriene, and (9Z, 12Z, 15Z)-octadecanetrienal in a mass ratio of 2:0.6:1). Five unmated male adults were placed in a small iron cage in the release area.

[0061] During the test, adult fall webworms were released from the leeward side. The number of fall webworms remaining in each area of ​​the wind tunnel after 5 minutes was observed and recorded. Each odorant was tested 6 times. Before the test, the adult webworms were placed in the wind tunnel to acclimatize for 5-10 minutes. Before each change of odor source, the inner wall of the wind tunnel was sprayed with anhydrous alcohol and wiped repeatedly to eliminate the residual effects of the volatile substances from the previous odor source.

[0062] All data were analyzed using Excel spreadsheets and SPSS 26.0. Charts were created using Origin 2024.

[0063] The results are as follows Figure 4 As shown, the odorant in the control group elicited a response in about 50% of unmated male adult fall webworms, the dodecane group elicited a response in about 80% of unmated male adult fall webworms, and the 1,4-diethylbenzene group elicited a response in 76.67% of unmated male fall webworms, significantly higher than the control group. Both dodecane and 1,4-diethylbenzene elicited significant behavioral responses in fall webworms.

[0064] Wind tunnel tests revealed that the addition of dodecane or 1,4-diethylbenzene significantly induced male adult American white moths to take flight and come into contact with the scent source, showing a significant difference from the control group (sex pheromones). Adding dodecane or 1,4-diethylbenzene to the sex pheromones resulted in better attraction of male adult American white moths.

[0065] 4. The effectiveness of attractants in trapping prey in the wild: 4.1 Overview of the test site: The experimental plot is located on County Road 024 in Yantai City, Shandong Province (37°31′9″ N, 121°26′21″ E). The forest stand in the plot is a green belt around the road, and the main forest type is poplar. It is an area where the American white moth occurs.

[0066] 4.2 Experimental Materials: Take 15 μL of the attractant provided in Examples 2, 8, 4, and 8, and add it to the carrier of the cuff-type rubber stopper to make a lure core, which serves as the treatment group. Take 15 μL of sex pheromone (the sex pheromone is a mixture of (3Z, 6Z, 9S, 10R)-9,10-epoxy-3,6-docosadiene, (3Z, 6Z, 9S, 10R)-9,10-epoxy-1,3,6-docosatriene and (9Z, 12Z, 15Z)-octadecanetrienal in a mass ratio of 2:0.6:1) and add it to the carrier of the cuff-type rubber stopper to make a lure core.

[0067] The traps were barrel-shaped. Five replicates were set up for the treatment and control groups. The traps were suspended at a height of 2 m, with a distance of 50 m between adjacent traps. The number of traps was checked and recorded every two days. The experiment lasted from August 26 to September 23, 2025.

[0068] 4.3 Data Processing and Analysis: All data were analyzed using Excel and SPSS 26.0. Charts were created using Origin 2024.

[0069] 4.4 Results: Results of field trapping Figure 5 As shown, the number of animals captured by adding the attractants from Examples 2, 8, 4, and 8 was higher than that of the control group, while the number of animals captured by adding the attractants from Comparative Examples 4 and 8 was not significantly different from that of the control group. However, the number of animals captured by adding the attractant from Example 8 was significantly higher than that of the control group, indicating that mixing 1,4-diethylbenzene with sex pheromones can improve the capture efficiency of sex attractants.

[0070] 5. The effects of odor compounds, sex pheromones, and attractants: The attractants, sex pheromones (sex pheromones dissolved in dichloromethane at a concentration of 10 mg / mL, the sex pheromones being a mixture of (3Z, 6Z, 9S, 10R)-9,10-epoxy-3,6-docosadiene, (3Z, 6Z, 9S, 10R)-9,10-epoxy-1,3,6-docosatriene and (9Z, 12Z, 15Z)-octadecanetrienal in a mass ratio of 2:0.6:1), dodecane (dissolved in dichloromethane at a concentration of 1 mg / mL), 1,4-diethylbenzene (dissolved in dichloromethane at a concentration of 1 mg / mL), 2-ethylhexanol (dissolved in dichloromethane at a concentration of 100 mg / mL), and m-xylene (dissolved in dichloromethane at a concentration of 1 mg / mL) from Examples 1, 7, and Comparative Examples 3 and 5 were subjected to EAG assays.

[0071] The attractants and sex pheromones provided in Examples 2, 8, 4, and 8 (the sex pheromones were (3Z,6Z, 9S, 10R)-9,10-epoxy-3,6-docosadiene, (3Z, 6Z, 9S, 10R)-9,10-epoxy-1,3,6-docosatriene, and (9Z, 12Z, 15Z)-octadecyltrienal was mixed in a mass ratio of 2:0.6:1, and 15 μL of dodecane, 1,4-diethylbenzene, 2-ethylhexanol and m-xylene were added dropwise to conduct wind tunnel behavior tests and field trapping tests. The amount of dodecane added to the carrier was the same as the amount of dodecane used when adding the attractant in Example 2, the amount of 1,4-diethylbenzene added to the carrier was the same as the amount of 1,4-diethylbenzene used when adding the attractant in Example 8, the amount of 2-ethylhexanol added to the carrier was the same as the amount of 2-ethylhexanol used when adding the attractant in Comparative Example 4, and the amount of m-xylene added to the carrier was the same as the amount of m-xylene used when adding the attractant in Comparative Example 8.

[0072] The experimental methods and data processing were the same as those in “1. Antennae Potential (EAG) Measurement”, “3. Wind Tunnel Behavioral Response of Unmated Male Adults of the Fall White Moth to the Attractant” and “4. Field Trapping Effect of the Attractant”. The results are shown in Table 1.

[0073] Table 1. Effects of odor compounds, sex pheromones, and attractants

[0074] As shown in Table 1, the combined use of odor compounds and sex pheromones in this invention significantly enhances the attraction of the attractant to the American white moth.

[0075] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A Malacosoma americanum attractant, characterized in that, The American white moth attractant comprises sex pheromones and odor compounds; the sex pheromones are composed of (3Z, 6Z, 9S, 10R)-9,10-epoxy-3, 6-heneicosadiene, (3Z, 6Z, 9S, 10R)-9,10-epoxy-1,3,6-heneicosatriene and (9Z, 12Z, 15Z)-octadecatrienal; and the odor compounds are dodecane or 1,4-diethylbenzene.

2. The M. sexta attractant of claim 1, wherein, The mass ratio of (3Z, 6Z, 9S, 10R)-9,10-epoxy-3, 6-heneicosadiene, (3Z, 6Z, 9S, 10R)-9,10-epoxy-1,3,6-heneicosatriene and (9Z, 12Z, 15Z)-octadecatrienal is 2:0.6:

1.

3. The M. sexta attractant of claim 1, wherein, The mass ratio of odor compounds to sex pheromones in the American white moth attractant is 0.1-10:

1.

4. The M. sexta attractant of claim 1, wherein, The American white moth attractant further comprises an organic solvent.

5. The M. sexta attractant of claim 1, wherein, The organic solvent is dichloromethane.

6. A method of attracting Malacosoma americanum comprising, The method comprises the following steps: The American white moth attractant is dropped into a carrier to make a lure core, the lure core is placed in a trap, and the trap is hung in a forest.

7. The method of claim 6, wherein, The height at which the trap is hung is ≥2 m.

8. The method of claim 6, wherein, The interval distance between two adjacent traps is 40-60 m.

9. Use of the American white moth attractant according to any one of claims 1-5 or the method according to any one of claims 6-8 in attracting American white moths.

10. Use according to claim 9, characterized in that, The American white moth attractant is used for attracting American white moth adults.

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