Compound microbial fertilizer and preparation method thereof

Abstract

The invention discloses a compound microbial fertilizer and a preparation method thereof, and the compound microbial fertilizer is prepared from the following raw materials in parts by weight: 100-150 parts of a bacillus siamensis microbial agent, 150-200 parts of a streptomyces pourpuensis microbial agent, 130-175 parts of a synergist, 185-215 parts of an auxiliary material, 110-140 parts of a chelate material, 41-78 parts of a catalyst, 10-30 parts of an active agent, 1-3 parts of an inhibitor, 0.5-1.5 parts of a thickening agent and 100-150 parts of water. The preparation method of the compound microbial fertilizer is simple and easy to implement, all the materials are added according to a specific sequence, it can be guaranteed that the materials are fully dissolved and mixed, the stability and uniformity of the fertilizer are guaranteed, and industrial production is facilitated; by applying the compound microbial fertilizer disclosed by the invention, the problem of lack of trace elements of crops can be directly solved, the growth and development of the crops are comprehensively optimized through the synergistic interaction of amino acids, the goals of quality improvement, yield increase, disease resistance and environmental protection are achieved, and the compound microbial fertilizer conforms to the development requirements of green, high-end and efficient agriculture and has wide popularization and application prospects.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of biological fertilizer, in particular to a compound microbial fertilizer and a preparation method thereof. BACKGROUND

[0002] With the continuous innovation of agricultural cultivation technology, water-soluble fertilizer has become an important input in modern agricultural production system due to its high nutrient absorption efficiency, strong application convenience, and precise nutrition supply. Among various types of water-soluble fertilizer, compound microbial fertilizer not only makes up for the shortcomings of single fertilizer, but also realizes the multi-dimensional improvement of "nutrient supply, soil improvement, crop health, and ecological environmental protection". The beneficial microorganisms in compound microbial fertilizer inhibit soil-borne diseases, and amino acids enhance the stress resistance of crops. The combination of the two reduces the use of pesticides by 20%-30%, reduces the pollution of pesticide residues to soil, water, and agricultural products, and protects the agricultural ecological environment. At the same time, amino acids can provide carbon source and nutrient substrate for microorganisms, promote their reproduction, and prolong the functional period of microorganisms. Amino acid molecules can effectively promote the active absorption and transport of mineral nutrients by crops, and improve their resistance to biological and abiotic stress through regulating physiological metabolic pathways. In recent years, it has gradually developed into a research frontier and hotspot in the field of fertilizer science.

[0003] However, there are some deficiencies in the compound microbial fertilizer on the market. The amino acid content in some products is low, which cannot fully play its due role. The formula design of some products is not reasonable, and the trace element matching is improper, which leads to poor absorption effect of crops. There are also some problems in the application process of some products, such as poor wetting effect of foliar spraying, large difference in effect of different application methods, etc., which affect the overall use effect of the fertilizer. In addition, in the process of fertilizer production, some added ingredients not only increase the production cost, but also do not produce obvious improvement on the effect of the fertilizer, causing waste of resources. Therefore, it is of great practical significance to develop a compound microbial fertilizer with high amino acid content, reasonable formula, good application effect, and controllable cost. SUMMARY

[0004] The first object of the present application is to provide a compound microbial fertilizer, and the second object of the present application is to provide a preparation method of the compound microbial fertilizer.

[0005] The first object of the present application is achieved by the following technical scheme: the raw materials include the following components in parts by weight: 100-150 parts of Bacillus siamensis YWF-25-21 bacterial agent, 150-200 parts of Streptomyces yangpuensis YWF-23-2312 bacterial agent, 130-175 parts of synergist, 185-215 parts of auxiliary material, 110-140 parts of chelating material, 41-78 parts of catalyst, 10-30 parts of active agent, 1-3 parts of inhibitor, 0.5-1.5 parts of thickening agent, and 100-150 parts of water; and the effective viable bacterial count in the compound microbial fertilizer is ≥5.0×10 8 CFU / mL.

[0006] The second object of the present application is achieved by the following technical scheme: a1, uniformly dispersing 0.5-1.5 parts of thickening agent in 100-150 parts of water to obtain an aqueous solution; a2, adding 5-15 parts of 3.5% polyglutamic acid to the aqueous solution of step a1 and mixing, then adding 5-10 parts of 50% aminobutyric acid and mixing, and then adding 30-60 parts of Ascophyllum nodosum and mixing; a3, on the basis of step a2, sequentially adding 120-150 parts of amino acid powder, 185-215 parts of auxiliary material, 110-140 parts of chelating material, and 10-15 parts of borax to the aqueous solution and mixing uniformly; then adding 10-30 parts of active agent and 1-3 parts of methyl benzoate; a4, on the basis of step a3, adding 100-150 parts of Bacillus siamensis YWF-25-21 bacterial agent and 150-200 parts of Streptomyces yangpuensis YWF-23-2312 bacterial agent and mixing uniformly to obtain the compound microbial fertilizer.

[0007] The present application has the following beneficial effects: 1. The compound microbial fertilizer has high free amino acid content and is rich in various trace elements (iron, manganese, copper, zinc, boron, molybdenum) and beneficial ingredients (polyglutamic acid, aminobutyric acid), which can provide sufficient and comprehensive nutrition for crop growth, effectively promote the absorption and utilization of nutrients by crops, enhance the photosynthesis of crops, and improve the quality and yield of crops; 2. The preparation method of the compound microbial fertilizer is simple and easy to implement, the materials are added in a specific order, which can ensure that the materials are fully dissolved and mixed, guarantee the stability and uniformity of the fertilizer, and facilitate industrialized production; 3. The compound microbial fertilizer also has good wetting effect, since sodium dodecyl sulfate (SDS) and polyoxyethylene octyl phenol ether-10 (OP-10) are added as auxiliary agents, when the fertilizer is sprayed on the leaves of crops, the fertilizer can uniformly wet the leaves, improve the absorption and utilization rate of the fertilizer, and avoid waste of the fertilizer due to poor wetting effect; 4. The application of the compound microbial fertilizer can directly solve the problem of trace element deficiency of crops, and through the synergistic effect of amino acids, it can optimize the growth and development of crops in all directions, achieve the goal of "quality improvement, yield increase, disease resistance and environmental protection", meet the development needs of green, high-end and efficient agriculture, and has broad application prospects. BRIEF DESCRIPTION OF DRAWINGS

[0008] Figure 1 Figure is the antagonism diagram of Streptomyces yanpuensis YWF-23-2312 and Phytophthora parasitica var. nicotianae; Figure 2 Figure is the leaf wetting effect comparison diagram; Figure 3 Figure is the growth promotion effect diagram of Bacillus siamensis YWF-25-21. DETAILED DESCRIPTION

[0009] The application will be further described below in combination with the drawings, examples, but is not limited to the contents of the specification.

[0010] A compound microbial fertilizer, by weight, comprises the following raw materials: Bacillus siamensis YWF-25-21 agent 100-150 parts, Streptomyces yanpuensis YWF-23-2312 agent 150-200 parts, synergist 130-175 parts, auxiliary material 185-215 parts, chelating material 110-140 parts, catalyst 41-78 parts, active agent 10-30 parts, inhibitor 1-3 parts, thickening agent 0.5-1.5 parts, and water 100-150 parts.

[0011] The Bacillus siamensis YWF-25-21 is preserved in the China Center for Type Culture Collection on July 11, 2025, and the preservation registration number is CCTCC M 20251577.

[0012] The Streptomyces yanpuensis YWF-23-2312 is preserved in the China Center for Type Culture Collection on September 7, 2023, and the preservation registration number is CCTCC M 20231648.

[0013] The synergist comprises at least two of amino acid powder 120-150 parts, 3.5% polyglutamic acid 5-15 parts, and 50% aminobutyric acid 5-10 parts.

[0014] The auxiliary material comprises at least two of potassium dihydrogen phosphate 95-105 parts and urea 90-110 parts.

[0015] The chelating agent includes at least two of chelated zinc 90-110 parts, chelated iron 3-5 parts, chelated manganese 15-20 parts, and chelated copper 2-5 parts.

[0016] The catalyst includes at least two of ammonium molybdate 1-3 parts, borax 10-15 parts, and bladderwrack 30-60 parts.

[0017] The active agent includes at least two of sodium dodecyl sulfate 5-15 parts and polyoxyethylene octyl phenol ether-10 5-15 parts.

[0018] The inhibitor is methyl paraben, and the thickening agent is xanthan gum.

[0019] A preparation method of a composite microbial fertilizer includes the following steps: a1, 0.5-1.5 parts of a thickening agent and 100-150 parts of water are added to a stirring tank, the stirring tank is started to stir at a speed of 300 r / min for 15 minutes until the xanthan gum is completely dispersed and no obvious particles are present, and a water solution is obtained; a2, the stirring tank is kept running, 5-15 parts of 3.5% polyglutamic acid are added to the water solution of step a1 and mixed, 5-10 parts of 50% aminobutyric acid are then added and mixed, and then 30-60 parts of bladderwrack are added and mixed thoroughly; a3, the stirring tank is kept running, on the basis of step a2, 120-150 parts of amino acid powder, 185-215 parts of an auxiliary material, 110-140 parts of a chelating agent, 10-30 parts of an active agent, and 1-3 parts of methyl paraben are sequentially added to the water solution and mixed thoroughly; a4, the stirring tank is kept running, on the basis of step a3, 100-150 parts of Bacillus siamensis YWF-25-21 inoculant and 150-200 parts of Streptomyces yanpuensis YWF-23-2312 inoculant are added and mixed thoroughly, and a composite microbial fertilizer is obtained; the effective viable bacterial count of the composite microbial fertilizer is ≥5.0×10 8 CFU / mL.

[0020] The Bacillus siamensis inoculant and the Streptomyces yanpuensis inoculant used in the preparation method of the composite microbial fertilizer are respectively prepared by fermentation culture.

[0021] Example 1 The preparation of the Bacillus siamensis YWF-25-21 inoculant includes the following steps: (1) Strain activation: under sterile conditions, the Bacillus siamensis YWF-25-21 strain was streaked and inoculated into LB solid medium plates, and cultured at 37 ℃ for 24 h. The formula of the LB solid medium was as follows: bacteriological peptone 10 g / L, yeast extract 5 g / L, sodium chloride 5 g / L, agar 20 g / L, and pH 7.0; (2) Strain propagation: the activated single colony was inoculated into LB liquid medium, and cultured at 37 ℃ and 180 r / min for 24 h to obtain a seed liquid; the seed liquid was inoculated into LB solid medium kelly bottles at a 4%-6% (v / v) inoculation amount, and cultured at 37 ℃ for 24 h, and then washed with sterile normal saline to obtain a strain liquid; (3) Fermentation culture: the strain liquid was inoculated into a fermentation medium at a 4%-6% (v / v) inoculation amount, and cultured at 37 ℃ and 180 r / min for 48 h, and then filtered to obtain the Bacillus siamensis YWF-25-21 microbial inoculant, with an effective viable cell count of ≥3×10 8 CFU / mL; (4) The formula of the fermentation medium included the following components: soybean meal powder 7.5 g / L, corn syrup dry powder 15.0 g / L, corn starch 10.0 g / L, glucose 5.0 g / L, yeast extract 2.5 g / L, peptone 4.5 g / L, magnesium sulfate 0.5 g / L, sodium chloride 0.25 g / L, potassium phosphate dibasic 0.7 g / L, calcium carbonate 3.0 g / L, vitamin B group 0.25 g / L, and the pH of the fermentation medium was 6.5, and the fermentation medium was sterilized at 121 ℃ and 0.1 MPa for 30 min.

[0022] Example 2 The preparation of the Streptomyces yanpuensis YWF-23-2312 microbial inoculant included the following steps: (1) Strain activation: under sterile conditions, the Streptomyces yanpuensis YWF-23-2312 strain was streaked and inoculated into PDA solid medium plates, and cultured at 28 ℃ for 48 h. The formula of the PDA solid medium was as follows: potato 200 g / L, glucose 20 g / L, and agar 20 g / L, and pH 7.0; (2) Strain propagation: the activated single colony was inoculated into PDA liquid medium, and cultured at 28 ℃ and 150 r / min for 48 h to obtain a seed liquid; (3) fermentation culture: inoculate the strain liquid into the fermentation medium at an inoculation amount of 4%~6% (v / v), and culture at 28 ℃ with 150 r / min shaking for 72 h. After the culture, filter to obtain the microbial inoculant of Streptomyces yanpuensis YWF-23-2312, and the effective viable cell number is ≥2×10 8 CFU / mL. (4) the formula of the fermentation medium includes: glucose 20.0 g / L, corn starch 15.0 g / L, yeast extract 2.5 g / L, magnesium sulfate 0.5 g / L, and dipotassium hydrogen phosphate 1.0 g / L. The pH of the fermentation medium is 6.5, and the fermentation medium is sterilized at 121 ℃, 0.1 MPa for 30 min.

[0023] Example 3 A compound microbial fertilizer, the compound microbial fertilizer includes the following raw materials by weight parts: Bacillus siamensis YWF-25-21 inoculant 100 parts, Streptomyces yanpuensis YWF-23-2312 inoculant 150 parts, synergist 130 parts, auxiliary material 185 parts, chelating material 110 parts, catalyst 41 parts, active agent 10 parts, inhibitor 1 part, thickening agent 0.5 part, and water 100 parts.

[0024] Example 4 A compound microbial fertilizer, the compound microbial fertilizer includes the following raw materials by weight parts: Bacillus siamensis YWF-25-21 inoculant 125 parts, Streptomyces yanpuensis YWF-23-2312 inoculant 175 parts, synergist 150 parts, auxiliary material 200 parts, chelating material 125 parts, catalyst 55 parts, active agent 20 parts, inhibitor 2 parts, thickening agent 1 part, and water 125 parts.

[0025] Example 5 A compound microbial fertilizer, the compound microbial fertilizer includes the following raw materials by weight parts: Bacillus siamensis YWF-25-21 inoculant 150 parts, Streptomyces yanpuensis YWF-23-2312 inoculant 200 parts, synergist 175 parts, auxiliary material 215 parts, chelating material 140 parts, catalyst 78 parts, active agent 30 parts, inhibitor 3 parts, thickening agent 1.5 parts, and water 150 parts.

[0026] Example 6 I. The antagonistic ability of Streptomyces yanpuensis YWF-23-2312 to the pathogen of tobacco black shank disease: The black shank pathogen was inoculated on PDA plates and cultured at 28°C for 5 days. A 5mm diameter puncher was used to take a bacterial cake at the edge of the colony, and the bacterial cake was inoculated at the center of another PDA plate. Two inoculation points were marked symmetrically at a distance of 2.5 cm from the center, and 10 μL of Yangpu Streptomyces YWF-23-2312 bacterial solution was taken to the two inoculation points using a 5mm puncher. The plates were incubated at 28°C for 3 days. The colony size was measured using a vernier caliper, and the inhibition rate was calculated according to the formula (inhibition rate (%) = 100 x (colony diameter of control pathogen - colony diameter of treated pathogen) ÷ colony diameter of control pathogen). The results showed that the inhibition rate of Yangpu Streptomyces YWF-23-2312 on the tobacco black shank pathogen was 65.34%.

[0027] II. Promoting effect of Bacillus siamensis YWF-25-21 The crop seeds were sterilized with 5% sodium hypochlorite for 10 min, washed with distilled water for 3 times, and then dried. 10 seeds were taken and placed in a 9 cm culture dish lined with 2 layers of sterile filter paper. 3 mL of Bacillus siamensis YWF-25-21 bacterial solution with an effective viable count of ≥2×10 8 CFU / mL was added to each dish, and the plates were placed in a light incubator for 4-5 days. The root length was measured using a vernier caliper, and the germination rate and germination index were calculated according to the formula.

[0028] Germination rate = (number of germinated seeds ÷ total number of seeds) x 100% Germination index = (treatment group germination rate x treatment group average root length) ÷ (blank control group germination rate x blank control group average root length) Table 1 Promoting effect of Bacillus siamensis

[0029] III. Preparation of a compound microbial fertilizer without Ascophyllum nodosum, which comprises, by weight: 140 parts of amino acid powder, 100 parts of potassium dihydrogen phosphate, 100 parts of urea, 100 parts of chelated zinc, 4 parts of chelated iron, 20 parts of chelated manganese, 3.5 parts of chelated copper, 2 parts of ammonium molybdate, 12 parts of borax, 10 parts of 3.5% polyglutamic acid, 4 parts of 50% aminobutyric acid, 10 parts of sodium dodecyl sulfate, 10 parts of polyoxyethylene octyl phenol ether-10, 1 part of methyl paraben, 125 parts of Bacillus siamensis YWF-25-21 inoculant, 150 parts of Yangpu Streptomyces YWF-23-2312 inoculant, and 207 parts of water. For convenience of subsequent description, the compound microbial fertilizer without Ascophyllum nodosum is named as Comparative Example 1.

[0030] Four, a catalyst and inhibitor-free composite microbial fertilizer preparation, by weight parts, comprising: amino acid powder 140 parts, potassium dihydrogen phosphate 100 parts, urea 100 parts, chelated zinc 100 parts, chelated iron 4 parts, chelated manganese 20 parts, chelated copper 3.5 parts, ammonium molybdate 2 parts, borax 12 parts, bladderwrack 50 parts, 3.5% polyglutamic acid 10 parts, 50% aminobutyric acid 4 parts, Bacillus siamensis YWF-25-21 inoculant 125 parts, Yangpu Streptomyces YWF-23-2312 inoculant 150 parts, water 178 parts. For the convenience of subsequent description, the catalyst and inhibitor-free composite microbial fertilizer is named as: Comparative Example 2.

[0031] Example 7 In order to better verify the use effect of the composite microbial fertilizer in Examples 3, 4 or 5, take Chinese cabbage as an example, and carry out comparative cultivation experiments in the greenhouse of the Microbial Fermentation Engineering Research Center of Yunnan Province.

[0032] Test method: the composite microbial fertilizer obtained in Example 1 is diluted and irrigated on Chinese cabbage with basically consistent growth vigor, irrigated once every 7 days, 1 L per plant, continuously irrigated for 3 times, 10 plants in each treatment group, 3 times of repetition, and the agronomic characters are investigated.

[0033] Three different treatment methods are set, as shown in Table 2 below, which are the composite microbial fertilizer containing Example 1 diluted 500 times, the composite microbial fertilizer not containing bladderwrack diluted 500 times, and water as a blank control.

[0034] Table 2 Treatment methods of each treatment group

[0035] The agronomic characters of each treatment group are determined, including plant height, chlorophyll, single plant weight, etc. The results are shown in Table 3.

[0036] Table 3 Agronomic characters of Chinese cabbage in each treatment group

[0037] As can be seen from the data in Table 3, the Chinese cabbage seedlings treated with the composite microbial fertilizer have a large increase in agronomic characters such as plant height, chlorophyll, and single plant weight, compared with the Chinese cabbage seedlings treated with the composite microbial fertilizer not containing bladderwrack in Comparative Example 1 and the blank control. It can be seen that bladderwrack contains a variety of bioactive substances, which can induce a series of positive physiological responses in plants, thereby promoting crop growth and increasing crop yield. The combination of bladderwrack and trace elements has a high application prospect in improving the utilization rate of fertilizer, improving the quality of crops, and increasing yield.

[0038] Example 8 Test method: The composite microbial fertilizer in example 3, 4 or 5 and the composite microbial fertilizer without catalyst and inhibitor in comparative example 2 are diluted 500 times respectively, and then foliar spraying is carried out on the leaves of lotus white, lemon and eggplant, and the wetting effect of the leaves is observed.

[0039] Leaf wetting effect: On lotus white leaves, the leaves sprayed without additives cannot be wetted, and the leaves sprayed with additives are in fine and small water droplet shape; on lemon leaves, the leaves sprayed without additives are in water droplet shape and easy to roll off, and the leaves sprayed with additives are evenly wetted; on eggplant, the leaves sprayed without additives are unevenly wetted and have small water droplets, and the leaves sprayed with additives are evenly wetted; therefore, the wetting effect of the composite microbial fertilizer with additives is better than that without additives.

[0040] Example 9 Test method: The composite microbial fertilizer obtained in example 3, 4 or 5 is diluted, and then the lettuce seedlings with basically consistent growth are selected for irrigation and spraying, taking water as a blank control, 5 treatments are set, 18 plants for each treatment, the remaining agricultural operations are kept consistent, the interval period is 7 days, and the treatment is continuously carried out for 3 times.

[0041] Table 4: Treatment method of each treatment group

[0042] The properties of lettuce, including plant height, chlorophyll content and single plant weight, are determined by using each treatment group in table 4. The results are shown in table 5.

[0043] Table 5: Lettuce agronomic properties of each treatment group

[0044] As can be seen from the data in table 5, the lettuce seedlings applied with the composite microbial fertilizer have a large increase in agronomic properties such as plant height, chlorophyll, single plant weight and the like compared with the composite microbial fertilizer without bubble leaf algae and the water control, and the effect of the method of irrigation after dilution is better than that of foliar spraying. It can be seen that for lettuce planting, the application of amino acid trace element fertilizer is a key technology integrating quality improvement, yield increase, disease resistance and environmental protection. It can directly solve the problem of trace element deficiency, and through the synergistic effect of amino acid, it can optimize the growth and development of lettuce in all directions, and finally realize higher economic benefit and ecological benefit, which is an ideal choice for the development of green, high-end and efficient agriculture.

[0045] In the technical scheme of the present application, the amino acid powder, potassium dihydrogen phosphate, urea, chelated zinc, chelated iron, chelated manganese, chelated copper, ammonium molybdate, borax, bubble leaf algae, 3.5% polyglutamic acid, 50% aminobutyric acid, sodium dodecyl sulfate, polyoxyethylene octyl phenol ether-10 and methyl paraben are all commercially available existing products.

[0046] The above detailed description has set forth various embodiments of the devices and / or processes described herein. It is to be understood, however, that the description is made by way of example only, not by way of limitation. Numerous modifications, adaptations, substitutions, and variations employing the principles set forth herein can be made by those skilled in the art without departing from the spirit and scope of the disclosure.

Claims

1. A compound microbial fertilizer, characterized in that, By weight, the raw materials include the following parts by weight: 100-150 parts of Bacillus sicca YWF-25-21 bacterial agent, 150-200 parts of Streptomyces yangpuensis YWF-23-2312 bacterial agent, 130-175 parts of synergist, 185-215 parts of excipients, 110-140 parts of chelating agent, 41-78 parts of catalyst, 10-30 parts of activator, 1-3 parts of inhibitor, 0.5-1.5 parts of thickener, and 100-150 parts of water.

2. The method for preparing the compound microbial fertilizer according to claim 1, characterized in that: The compound microbial fertilizer contains ≥5.0 × 10⁻⁶ effective viable bacteria. 8 CFU / mL.

3. The compound microbial fertilizer according to claim 1, characterized in that: The synergist includes at least two of the following: 120-150 parts of amino acid powder, 5-15 parts of 3.5% polyglutamic acid, and 5-10 parts of 50% aminobutyric acid.

4. The compound microbial fertilizer according to claim 1, characterized in that: The excipients include at least two of the following: 95-105 parts potassium dihydrogen phosphate and 90-110 parts urea.

5. The compound microbial fertilizer according to claim 1, characterized in that: The chelating material includes at least two of the following: 90-110 parts of chelated zinc, 3-5 parts of chelated iron, 15-20 parts of chelated manganese, and 2-5 parts of chelated copper.

6. The compound microbial fertilizer according to claim 1, characterized in that: The catalyst comprises at least two of the following: 1-3 parts ammonium molybdate, 10-15 parts borax, and 30-60 parts of *Alternaria latifolia*.

7. The compound microbial fertilizer according to claim 1, characterized in that: The active agent includes at least two of the following: sodium dodecyl sulfate (5-15 parts) and polyoxyethylene octylphenol ether-10 (5-15 parts).

8. A method for preparing a compound microbial fertilizer according to any one of claims 1 to 7, characterized in that, Includes the following steps: a1. Disperse 0.5 to 1.5 parts of thickener evenly in 100 to 150 parts of water to obtain an aqueous solution; a2. Add 5-15 parts of 3.5% polyglutamic acid to the aqueous solution of step a1 and mix well, then add 5-10 parts of 50% aminobutyric acid and mix well, then add 30-60 parts of *Leptochloa crus-galli* and mix well. a3. Based on step a2, add 120-150 parts of amino acid powder, 185-215 parts of excipients, 110-140 parts of chelating agent, and 10-15 parts of borax to the aqueous solution in sequence, and mix evenly; then add 10-30 parts of activator and 1-3 parts of methyl benzoate. a4. Based on step a3, add 100-150 parts of Bacillus sicca YWF-25-21 bacterial agent and 150-200 parts of Streptomyces yangpuensis YWF-23-2312 bacterial agent and mix evenly to obtain compound microbial fertilizer.

9. The method for preparing the compound microbial fertilizer according to claim 8, characterized in that: The *Bacillus sicca* YWF-25-21 and *Streptomyces yangpuensis* YWF-23-2312 bacterial agents were prepared by fermentation culture, respectively. The effective viable count of the *Bacillus sicca* YWF-25-21 bacterial agent was ≥3×10⁻⁶. 8 CFU / mL, the effective viable count of the Yangpu Streptomyces YWF-23-2312 inoculum is ≥2×10⁻⁶. 8 CFU / mL.

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