Intertidal zone benthic microalgae multispectral adaptive carbon sequestration rate in-situ monitoring system and intertidal zone benthic microalgae multispectral adaptive carbon sequestration rate in-situ monitoring method

Abstract

The invention relates to an intertidal zone benthic microalgae multispectral self-adaptive carbon sequestration rate in-situ monitoring system and method, the system comprises a reactor used for containing intertidal zone sediments, the top of the reactor is covered with a cover plate, and the cover plate is provided with an LED lamp; a through hole is formed in the side wall of the reactor, an electrode penetrates through the through hole, and the other end of the electrode is electrically connected with a monitor; a micro-motor system is further arranged on the side wall of the reactor, and the micro-motor system is electrically connected with the LED lamp so as to control on and off of the LED lamp; a water changing opening is further formed in the side wall of the reactor, and the water changing opening is electrically connected with the micro-motor system so as to control opening and closing of the water changing opening; the scheme provided by the invention has the advantages that the method is not influenced by the environment, and the carbon sequestration rate of the benthic microalgae can be measured at high precision in any adverse environment.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of environmental biotechnology, and specifically relates to a benthic microalgae carbon fixation monitoring and ecological carbon sink evaluation technology, in particular to determination of in-situ carbon fixation rate of intertidal benthic microalgae, namely an intertidal benthic microalgae multi-spectral adaptive carbon fixation rate in-situ monitoring system and method. BACKGROUND

[0002] As a primary producer in wetland ecosystems, the carbon fixation capacity of intertidal benthic microalgae (tidal flat microbenthic algae) not only directly affects the health and stability of the wetland ecosystem, but also indirectly affects the mitigation potential of global climate change. A comprehensive understanding of the carbon fixation rate of benthic microalgae provides scientific basis for carbon sink measurement, ecological restoration and pollution control, and helps to achieve the synergy of the "double carbon" goal and high-quality economic development.

[0003] Currently, the determination of the carbon fixation rate of intertidal benthic microalgae mainly uses in-situ bell jar method, which relies on natural light and cannot determine the photosynthetic rate in weak light environments such as cloudy days and thunderstorms. In addition, the dark reaction requires manual shading, and the touch of the instrument to the sediment during shading will disturb the benthic microalgae community structure, resulting in measurement error of the respiration rate. During manual water change, the water flow impact will destroy the balance of the sediment-water interface, affecting the accuracy of carbon flux data. The traditional determination method and equipment have large measurement error of the carbon fixation rate of benthic microalgae, and have narrow application, which has limitations in various ecological environments. Therefore, there is an urgent need for innovative technology to provide more convenient and accurate determination technology.

[0004] Intertidal zone belongs to the field, and the determination of carbon fixation rate is greatly affected by environmental factors. Because of the huge carbon sink effect of intertidal zone, the demand for determination of carbon fixation rate of intertidal zone is more urgent. It is also necessary to seek equipment and methods to overcome adverse environmental factors and improve measurement accuracy, which provides help for carbon sink measurement, ecological restoration, etc. SUMMARY

[0005] The present application provides an intertidal benthic microalgae multi-spectral adaptive carbon fixation rate in-situ monitoring system which can accurately determine the carbon fixation rate of benthic microalgae in any adverse environment without being affected by the environment.

[0006] In order to solve the above technical problems, the technical scheme adopted by the present application is: an intertidal zone benthic microalgae multi-spectral adaptive carbon fixation rate in-situ monitoring system, the system comprises a reactor for containing intertidal zone sediments, the top of the reactor is provided with a cover plate, the cover plate is provided with an LED lamp, the side wall of the reactor is provided with a through hole, the through hole is used for inserting an electrode, the other end of the electrode is electrically connected with a monitor, the side wall of the reactor is further provided with a micro-electromechanical system, the micro-electromechanical system is electrically connected with the LED lamp to control the opening and closing, and the side wall of the reactor is further provided with a water exchange port, the water exchange port is electrically connected with the micro-electromechanical system to control the opening and closing of the water exchange port.

[0007] By using the above system, the intertidal zone sediments are placed in the reactor, the reactor is filled with water, the opening and closing of the LED lamp is controlled by the micro-electromechanical system to simulate sunlight, and the water exchange port is controlled by the micro-electromechanical system to simulate the ebb and flow of seawater; then the dissolved oxygen value of the intertidal zone sediments is more accurately measured by the electrode, the system structure is stable, even in weak light environment such as cloudy days and thunderstorms, the photosynthetic rate can be measured through the setting of the LED lamp, and manual shading is not required in the dark reaction, so that the situation that the instrument touches the sediments and disturbs the benthic microalgae community structure during shading process does not occur, causing the respiration rate measurement error; the water exchange is automatically performed by the micro-electromechanical system in the present application, so that the situation that the water flow impact destroys the balance of the sediment-water interface does not occur; therefore, the carbon fixation rate measurement process of the above system of the present application is not easily affected by environmental factors, and the intertidal zone benthic microalgae carbon fixation rate can be more accurately measured; in addition, the electrode of the present application can adopt the optical detection principle of the fluorescence probe to avoid the interference of sulfides and other substances on the electrochemical electrode, the response time reaches seconds, and the measurement accuracy is improved.

[0008] Further, the electrode is a fluorescence probe or a general probe, the electrode is located above the intertidal zone sediments, and after water injection, the electrode is located in the water body; by using this structure, the dissolved oxygen value can be more accurately measured, if the distance from the sediment surface is too close or in contact, the oxygen bubbles generated by the benthic microalgae may be directly attached to the electrode probe, causing the value to be too high, and by increasing the distance, the oxygen bubbles will dissolve in the water, and the value will be more accurate.

[0009] Further, the electrode is located above the intertidal zone sediments by more than 2 cm, and the opposite side of the electrode is a sunny side; by using this structure, the dissolved oxygen value can be more accurately measured by the probe, if the distance from the sediment surface is too close or in contact, the oxygen bubbles generated by the benthic microalgae may be directly attached to the electrode probe, causing the value to be too high, and by increasing the distance, the oxygen bubbles will dissolve in the water, and the value will be more accurate.

[0010] Further, the LED lamp is an integrated LED array lamp, which can simulate different light intensity and spectrum, break through the dependence on natural light in the traditional method, and realize all-weather determination.

[0011] Further, the reactor is a transparent reactor, which can use natural light when the sunlight is good, and the transparent structure does not block the natural light. In addition, the transparent material can facilitate observation of the situation in the container.

[0012] Further, the reactor is a cuboid reactor, the perforations are located on the side wall of the long side of the reactor, and the micro-electromechanical system is located on the side wall of the short side of the reactor. The structure can ensure that the probe has sufficient light to irradiate, and the micro-electromechanical system arranged on the side wall of the short side can avoid blocking the light, so that the intertidal sediment reaction in the reactor is more thorough, and the detection is more accurate.

[0013] The application also provides a method for determining the intertidal benthic microalgae multi-spectrum adaptive carbon sequestration rate in-situ monitoring system. (1) First, take the surface 0-1cm thick sediment of the intertidal benthic microalgae in the natural environment, the sediment surface has no obvious plants and animals, and the sediment length and width are adapted to the inner wall of the reactor, and the sediment is put into the reactor; (2) The electrode is inserted into the reactor through the rubber plug, the fluorescence probe is located above the sediment, and the other end of the electrode is connected to the external monitor (which can be a CO2 and O2 integrated measuring instrument); (3) Water is injected into the reactor so that the water can fill the gap of the sediment without leaving gaps, and then the reactor is sealed with transparent sealing glue, and placed at the sampling point; (4) Set the light intensity and illumination time of the LED array lamp built-in the cover plate above the reactor, turn on the monitor and the micro-electromechanical system on the side of the container, set the water change time synchronized with the tide, and record the related data in real time.

[0014] Further, the electrode in step (2) of the application includes a fluorescence probe and a general electrode probe (optical and electrochemical method), and the probe is higher than the sediment by 2cm or more. The probe collects pH, temperature, chlorophyll fluorescence and other data every 15-30 minutes.

[0015] Further, the reactor in step (2) of the application is a transparent container, including but not limited to glass material, acrylic material, polycarbonate material, polytetrafluoroethylene material, etc.

[0016] Further, in step (3) of the present application, seawater is injected by a syringe, and the syringe injects seawater along the inner wall of the reactor, avoiding disturbance of the sediment and causing turbidity of seawater, thereby affecting the photosynthetic rate to be determined.

[0017] Further, in step (4) of the present application, the ratio of light exposure time to dark reaction time is 12 h:12 h or 16 h:8 h.

[0018] Further, in step (4) of the present application, the water exchange time controls the water exchange port to be opened half an hour before the high tide, and the seawater in the container is emptied. After the container is filled with seawater after the high tide, the water exchange port is closed half an hour later, so that the dynamic balance of nutrients, O2 and CO2 in the container is suitable for the survival of microalgae.

[0019] Further, in step (4) of the present application, the light intensity of the LED lamp can be set to 500-30000 Lux, and the LED lamp includes blue, red and other wave bands, which can adaptively adjust the spectrum combination according to the ambient light intensity.

[0020] Further, in step (4) of the present application, after the seawater is emptied by the micro-electromechanical system, the change of carbon flux is determined by using 15 minutes within half an hour. Advantages and beneficial effects of the present application:

[0021] 1. The present application breaks through the dependence on natural light of the traditional method by arranging an integrated LED array and built-in chips, reduces manual operation, can long-term monitoring, and realizes 24 / 7 full-cycle data capture.

[0022] 2. The fluorescence probe used in the present application is not affected by water flow rate, sulfide and other electrochemical interference, and the response time is seconds, so the determination is more accurate.

[0023] 3. The monitor of the present application can simultaneously realize the dual monitoring of dissolved oxygen concentration and carbon dioxide concentration, and improve the monitoring efficiency.

[0024] 4. The micro-electromechanical system of the present application controls the water exchange port, which does not need manual operation, is convenient for long-term monitoring, is beneficial to the survival of benthic microalgae, and the carbon fixation rate of benthic microalgae is more accurate.

[0025] 5. The micro-electromechanical system of the present application can also capture multiple parameters such as pH, temperature and chlorophyll fluorescence, so that the multiple parameters and the carbon fixation rate correspond in real time, which is beneficial to the analysis of the data in the later stage and improves the determination accuracy of the carbon fixation rate. BRIEF DESCRIPTION OF DRAWINGS

[0026] Fig. 1 The structure diagram of the present application is shown in the structure diagram of the present application.

[0027] Fig. 2The structural diagram of the reactor of the present application.

[0028] As shown in the drawings: 1. reactor, 101. perforation, 102. water exchange port, 2. cover plate, 3. LED lamp, 4. electrode, 5. monitor, 6. micro electro mechanical system, 7. intertidal sediment. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present application will be described clearly and completely below in combination with the embodiments and drawings. Obviously, the described embodiments are only preferred embodiments, but not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work belong to the protection scope of the present application; Furthermore, it should be understood that the description of the structural device part of the present application: when a component is referred to as "fixed to" another component, it can be directly on the other component or there can be another intermediate component fixed through the intermediate component. When a component is referred to as "connected to" another component, it can be directly connected to the other component or there can be another intermediate component. When a component is referred to as "provided on" another component, it can be directly provided on the other component or there can be another intermediate component. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the present application belongs. The terms used in the specification of the present application are only for the purpose of describing the specific embodiments and are not intended to limit the present application. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0030] As shown in the drawings: Figs. 1-2As shown, this application discloses an in-situ monitoring system for the multispectral adaptive carbon fixation rate of intertidal benthic microalgae. The system includes a reactor 1 for storing intertidal sediment 7. The reactor 1 is topped with a cover plate 2, on which an LED light 3 is mounted (the cover plate 2 is also transparent to ensure that the light from the LED light can completely penetrate and illuminate the intertidal sediment 7 inside the reactor). The sidewall of the reactor 1 has perforations 101 through which electrodes 4 are inserted. The other end of the electrodes 4 is electrically connected to a monitoring device. Instrument 5; A micro-motor system 6 is also provided on the side wall of the reactor 1 (the micro-motor system 6 can be built into the reactor or placed on the outside of the reactor), the micro-motor system 6 is electrically connected to the LED light 3 to control its opening and closing; a water exchange port 102 is also provided on the side wall of the reactor 1, the water exchange port 102 is electrically connected to the micro-motor system 6 to control the water exchange port to open and close automatically (specifically, the water exchange valve inside the water exchange port 102 is electrically connected to the micro-motor system 6); specifically, as an example, see Figure 1. As shown in the figure, the LED array light of this application is installed on the transparent cover plate 2 on the upper side of the transparent reactor 1. It can be electrically connected to the control module of the micro-motor system 6 through wires to control its switching, illumination time setting, illumination intensity, etc. The micro-motor system 6 of this application is fixed on the side wall of the short side of the reactor, and its drive end is connected to the water exchange valve in the water exchange port 102 provided on the reactor 1. The electrode 4 of this application is fixed to the reactor 1 through the side perforation 101 and the rubber stopper (the rubber stopper is located in the perforation to play a sealing role). The detection end of the electrode 4 is located at the upper left or upper right corner of the reactor 1, and is 2cm or more away from the surface of the sediment. The other end of the electrode 4, i.e. the signal end, is connected to the monitoring instrument 5 through a cable. The monitoring instrument 5 has built-in sensors such as CO2 / O2 and can store data in real time. The reactor 1 of this application can be a rectangular transparent glass container as shown in the figure, with a length of 20cm, a width of 10cm, and a height of 15cm. Figs. 1-2 This is a schematic diagram of the system described above in this application. The dashed lines represent the structures inside the reactor. To present the structure and location of each component in this application more completely, the internal visible representation is used. In addition, the rubber plug of this application is fitted on the probe and located inside the perforation 101 to prevent leakage from this point after seawater is injected, thus achieving a sealing effect.

[0031] The system is used to place the intertidal zone sediment in the reactor, then water is injected into the reactor, the opening and intensity change of the LED lamp are controlled by the micro motor system to simulate sunlight, and the water inlet is controlled by the micro motor system to simulate the ebb and flow of the sea; then the dissolved oxygen value of the intertidal zone sediment is more accurately measured by the electrode, the system structure is stable, even in weak light environment such as overcast day, thunderstorm, etc. The photosynthetic rate can be measured by setting the LED lamp, and the dark reaction does not need manual shading, so there will be no situation that the instrument touches the sediment during shading, which will disturb the benthic microalgal community structure and cause the determination error of the respiration rate. The water is automatically replaced by the micro motor system, which will not cause the water flow to impact and destroy the balance of the sediment-water interface. Therefore, the determination process of the carbon fixation rate of the above-mentioned system is not easily affected by environmental factors, and the determination of the carbon fixation rate of the intertidal benthic microalgae can be more accurate. In addition, the fluorescence probe adopts optical detection principle, avoids the interference of sulfide and other substances on the electrochemical electrode, and the response time reaches seconds, which improves the determination accuracy.

[0032] As an example, the electrode 4 described in the application is a fluorescence probe or a general probe, which is located above the intertidal zone sediment 7 and is located in the water after water injection; by using this structure, the dissolved oxygen value can be more accurately measured. If the distance from the sediment surface is too close or in contact, the oxygen bubbles generated by the benthic microalgae may directly adhere to the electrode probe, causing the value to be too high. By increasing the distance, the oxygen bubbles will dissolve in the water, and the value will be more accurate. The example adopts a fluorescence probe.

[0033] As an example, the electrode 4 described in the application is located above the intertidal zone sediment by more than 2 cm, and the opposite side of the electrode 4 is the sunny side; by using this structure, the dissolved oxygen value can be more accurately measured by the probe. If the distance from the sediment surface is too close or in contact, the oxygen bubbles generated by the benthic microalgae may directly adhere to the electrode probe, causing the value to be too high. By increasing the distance, the oxygen bubbles will dissolve in the water, and the value will be more accurate.

[0034] As an example, the LED lamp 3 described in the application is an integrated LED array lamp, which can simulate different light intensity and spectrum, break through the dependence on natural light in traditional methods, and realize all-weather determination.

[0035] As an example, the reactor described in the application is a transparent reactor; by using this structure, natural light can be used when the sun is good on sunny days, and the transparent setting will not block the natural light; secondly, the transparent material can also facilitate observation of the situation in the container during the period.

[0036] As shown in the accompanying drawings Figs. 1-2As shown, the reactor 1 described in the present application is a cuboid reactor, the perforations 101 are located on the long side of the reactor 1, and the micro-electromechanical system 6 is located on the short side of the reactor 1; with this structure, the probe can be ensured to have sufficient light to irradiate, and the micro-electromechanical system is arranged on the short side of the wall to avoid blocking the light, so that the intertidal sediment reaction in the reactor is more thorough, and the detection is more accurate.

[0037] As an example, the LED array lamp described in the present application is three rows and five columns.

[0038] As an example, the monitor described in the present application can simultaneously monitor the concentrations of dissolved oxygen and carbon dioxide.

[0039] As an example, the transparent container described in the present application is a cuboid container.

[0040] As an example, the micro-electromechanical system described in the present application controls the switching time of the water inlet and each parameter monitoring.

[0041] As shown in the accompanying drawings Figs. 1-2 In order to more clearly and accurately express the structure of the system of the present application, the structure inside the reactor is represented by a dashed line; the reactor of the present application is a cuboid structure, and the sediment is directly located inside.

[0042] The following is a specific embodiment of in-situ monitoring of carbon sequestration rate using the above-mentioned intertidal benthic microalgae multi-spectral adaptive carbon sequestration rate in-situ monitoring system.

[0043] Specifically, according to Fig. 1The device shown carries out in-situ monitoring of the carbon fixation rate, specifically as follows: in May, when measuring the carbon fixation rate of the intertidal zone of Hangzhou Bay and Beilun coast, the surface 1 cm thick sediment was first taken with a spatula, the surface of the sediment had no obvious plants and animals, the length and width were equal to the reactor, and the reactor was placed in the reactor. The size of the reactor (length x width x height is 20 cm x 10 cm x 15 cm). The spatula is made of polytetrafluoroethylene to avoid metal pollution, and the sampling is carried out along the horizontal direction. The fluorescence probe is inserted into the reactor through the rubber plug, the probe is far above the sediment surface, and is located at the upper left corner of the container. The water is injected into the reactor with a syringe, the water in the interstitial space of the sediment is filled, and no gap is left. The reactor is sealed with transparent sealing glue and placed at the sampling point. The built-in array lamp is set, the specific spectral parameters of the LED array lamp (blue band wavelength 450-470 nm, red band 620-660 nm), the arrangement mode of "three rows and five columns" (spacing 5 cm). The light intensity is 10000Lux, the light time is 12h, and the dark reaction time is 12h. Turn on the monitor and the micro motor system on the side of the container, and trigger the water exchange port switch through the tidal sensor. The response time of the switch is ≤10s, set the water exchange time synchronously with the tide, and open the water exchange port half an hour before the tide. Empty the seawater in the container, and after the container is filled with seawater after the tide, close the water exchange port half an hour later, and turn on the monitor to record the relevant data in real time. To be more accurate, three instruments are set for each sampling site, and continuous monitoring is carried out for one week.

[0044] In the examples of the present application, the monitor used is a model YSI EXO2 monitor, which is configured with basic sensors (temperature, dissolved oxygen, pH) and is produced and sold by YSI Company of the United States; the CO2 probe used in the probe of the present application is a German AMT, and the chlorophyll fluorescence online monitoring probe used is Yushan Y515-A.

[0045] The micro motor system of the present application uses a model BKL10 micro motor produced by Meibei Sanmei, Oase EAC controller, which can control the water pump and LED and supports mobile phone APP operation. Example 1

[0046] The example is the determination of carbon fixation rate of Spartina in the intertidal zone along the coast of Hangzhou Bay in May. The carbon fixation rate was measured in the actual continuous operation for 7 days in the Spartina sampling site. The first device measured the carbon fixation rate as 1.72, 1.79, 1.75, 1.71, 1.74, 1.75, and 1.80 g / (m2*d), the second device measured the carbon fixation rate as 1.79, 1.82, 1.76, 1.83, 1.85, 1.81, and 1.79 g / (m2*d), and the third device measured the carbon fixation rate as 1.77, 1.75, 1.82, 1.78, 1.74, 1.79, and 1.81 g / (m2*d). The standard deviation of the carbon fixation rate measured by each device for 7 days was 0.0312, 0.0296, and 0.0283, respectively.

[0047] The traditional carbon fixation rate determination method (measuring the dissolved oxygen concentration in seawater with an electrode to calculate the carbon dioxide rate, but only one data can be measured in a single line, and it is affected by weather, geographical conditions, precision, manual operation, and other factors. Alternatively, the traditional bell jar method is used to measure the carbon dioxide concentration change in the container to obtain the carbon fixation rate, but it can only be measured on the surface of the sediment after the tide recedes, and it cannot measure the carbon fixation rate of benthic microalgae after the tide rises). The carbon fixation rate (measured in carbon) was measured in the actual continuous operation for 7 days in the Spartina sampling site. The first device measured the carbon fixation rate as 1.51, 1.87, 0.91, 1.27, 1.89, 1.93, and 0.85 g / (m2*d), the second device measured the carbon fixation rate as 1.62, 1.78, 0.99, 1.39, 1.76, 1.91, and 0.95 g / (m2*d), and the third device measured the carbon fixation rate as 1.57, 1.66, 1.01, 1.59, 1.65, 1.93, and 0.96 g / (m2*d). The standard deviation of the carbon fixation rate measured by each device for 7 days was 0.4379, 0.3668, and 0.4051, respectively. On the third and seventh days of the seven days, there was thunderstorm weather, and the benthic microalgae only had a respiration rate. On the fourth day, it was cloudy, and the photosynthesis rate was low. Example 2

[0048] The example takes the determination of carbon sequestration rate in the intertidal zone of Hangzhou Bay in May as an example. The carbon sequestration rate is measured in the actual continuous operation of 7 days in the sample site of the intertidal zone. The first device measures the carbon sequestration rate of 2.21, 2.09, 2.13, 2.19, 2.18, 2.11, and 2.20 g / (m2*d), respectively. The second device measures the carbon sequestration rate of 2.16, 2.11, 2.19, 2.23, 2.12, 2.06, and 2.17 g / (m2*d), respectively. The third device measures the carbon sequestration rate of 2.23, 2.15, 2.17, 2.21, 2.14, 2.19, and 2.24 g / (m2*d), respectively. The standard deviation of the carbon sequestration rate measured by each device for seven days is 0.0478, 0.0564, and 0.0387, respectively.

[0049] The carbon sequestration rate is measured in the actual continuous operation of 7 days in the sample site of the intertidal zone. The first device measures the carbon sequestration rate of 1.95, 2.19, 1.55, 1.69, 2.29, 2.01, and 1.48 g / (m2*d), respectively. The second device measures the carbon sequestration rate of 2.04, 2.26, 1.41, 1.72, 1.96, 2.13, and 1.52 g / (m2*d), respectively. The third device measures the carbon sequestration rate of 1.99, 2.08, 1.57, 1.68, 2.09, 2.23, and 1.45 g / (m2*d), respectively. The standard deviation of the carbon sequestration rate measured by each device for seven days is 0.342, 0.319, and 0.290, respectively. Example 3

[0050] The example takes the determination of carbon sequestration rate in the intertidal zone of Hangzhou Bay in May as an example. The carbon sequestration rate is measured in the actual continuous operation of 7 days in the sample site of the intertidal zone. The first device measures the carbon sequestration rate of 1.62, 1.57, 1.60, 1.63, 1.58, 1.61, and 1.59 g / (m2*d), respectively. The second device measures the carbon sequestration rate of 1.55, 1.63, 1.61, 1.60, 1.58, 1.57, and 1.62 g / (m2*d), respectively. The third device measures the carbon sequestration rate of 1.64, 1.60, 1.58, 1.59, 1.63, 1.61, and 1.57 g / (m2*d), respectively. The standard deviation of the carbon sequestration rate measured by each device for seven days is 0.021, 0.028, and 0.025, respectively.

[0051] The carbon fixation rate (in carbon) is measured in the sample plot of the grass by using the traditional carbon fixation rate determination method for actual continuous operation for 7 days. The carbon fixation rate determined by the first device is 1.72, 1.66, 1.01, 1.44, 1.59, 1.63, and 0.98 g / (m2*d), the carbon fixation rate determined by the second device is 1.78, 1.53, 0.91, 1.39, 1.61, 1.75, and 1.03 g / (m2*d), and the carbon fixation rate determined by the third device is 1.82, 1.69, 0.96, 1.42, 1.64, 1.67, and 1.07 g / (m2*d). The standard deviation of the carbon fixation rate monitored by each device for seven days is 0.276, 0.299, and 0.307, respectively.

[0052] According to the comparison of the determination of the carbon fixation rate by the two devices in each of the above specific embodiments, the results show that the monitoring device error of the determination system of the present application is small, the stability is high, the thunderstorm and cloudy weather are overcome, and continuous monitoring can still be performed. At the same time, continuous seven days do not require manual operation, and all monitoring can be completed only by the device itself. The device of the traditional test method is affected by thunderstorm and cloudy weather, and cannot effectively monitor. The measurement error is large, and manual operation is required to replace seawater and copy data every day, which is more time-consuming and laborious. Through the comparison of the monitoring of the carbon fixation rate by the devices of the two test methods, the experimental results are directly compared and demonstrated to prove that the technical scheme of the present application has a more ideal carbon fixation rate monitoring effect.

Claims

1. An intertidal benthic microalgae multi-spectral adaptive carbon fixation rate in-situ monitoring system, characterized in that: The system comprises a reactor (1) for containing intertidal zone sediments (7), a cover plate (2) is arranged on the top of the reactor (1), and an LED lamp (3) is arranged on the cover plate (2); a perforation (101) is arranged on the side wall of the reactor (1), and an electrode (4) penetrates through the perforation (101); the other end of the electrode (4) is electrically connected with a monitor (5); a micro electromechanical system (6) is further arranged on the side wall of the reactor (1), and the micro electromechanical system (6) is electrically connected with the LED lamp (3) to control the opening and closing; a water exchange port (102) is further arranged on the side wall of the reactor (1), and the water exchange port (102) is electrically connected with the micro electromechanical system (6) to control the opening and closing of the water exchange port (102).

2. The intertidal benthic microalgae multispectral adaptive fluorescence carbon fixation rate in-situ monitoring system according to claim 1, characterized in that: The electrode (4) is a fluorescence probe or a common probe, and the electrode (4) is located above the intertidal zone sediments (7), and after water injection, the electrode (4) is located in the water body.

3. The intertidal benthic microalgae multispectral adaptive flame carbon fixation rate in-situ monitoring system according to claim 2, characterized in that: The electrode (4) is located above the intertidal zone sediments (7) by more than 2 cm, and the opposite side of the electrode (4) is the sunny side.

4. The intertidal benthic microalgae multispectral adaptive flame carbon fixation rate in-situ monitoring system according to claim 1, characterized in that: The LED lamp is an integrated LED array lamp; and the reactor (1) is a transparent reactor.

5. The intertidal benthic microalgae multispectral adaptive flame carbon fixation rate in-situ monitoring system according to claim 1, characterized in that: The reactor (1) is a cuboid reactor, the perforation (101) is arranged on the side wall of the long side of the reactor (1), and the micro electromechanical system (6) is arranged on the side wall of the short side of the reactor (1).

6. A method for determining the carbon sequestration rate of intertidal benthic microalgae using the multispectral adaptive estimation system of any one of claims 1-5. The steps of the method comprise: (1) First, take the surface layer 0-1cm thick of the intertidal zone benthic microalgae in the natural environment, the surface of the sediment has no obvious plants and animals, and the length and width of the sediment are adapted to the inner wall of the reactor, and the sediment is put into the reactor; (2) the electrode is inserted into the reactor through the rubber plug, the fluorescence probe is located above the sediment, and the other end of the electrode is connected with the external monitor; (3) water is injected into the reactor so that the water can fill the gap of the sediment without leaving a gap, and then the reactor is sealed with transparent sealing glue, and placed at the sampling point; (4) set the light intensity and light time of the built-in LED array lamp on the cover plate above the reactor, turn on the monitor and the micro electromechanical system on the side of the container, set the water exchange time synchronized with the tide, and record the related data in real time.

7. The method for determining the intertidal benthic microalgae multispectral adaptive estimation carbon fixation rate in-situ monitoring system according to claim 6, characterized in that: The electrode in step (2) includes a fluorescence probe and a common electrode probe, and the probe is higher than the sediment by more than 2cm; the reactor in step (2) is a transparent container.

8. The method for determining the intertidal benthic microalgae multi-spectral adaptive carbon fixation rate in-situ monitoring system according to claim 6, characterized in that: In step (3), a syringe is used to inject seawater, and the syringe injects seawater along the inner wall of the reactor.

9. The method for determining the intertidal benthic microalgae multi-spectral adaptive carbon fixation rate in-situ monitoring system according to claim 6, characterized in that: In step (4), the light time and dark reaction time ratio is 12h:12h or 16h:8h; in step (4), the water exchange port is opened half an hour before the tide, the seawater in the reactor is discharged, and after the reactor is filled with seawater after the tide, the water exchange port is closed half an hour later, so that the nutrient substances, O2 and CO2 in the reactor are in dynamic balance suitable for the survival of microalgae.

10. The method for determining the intertidal benthic microalgae multi-spectral adaptive carbon fixation rate in-situ monitoring system according to claim 6, characterized in that: The light intensity of LED lamp in step (4) is set to 500-30000 Lux, and the LED lamp contains blue and red wave bands; in step (4), the water replacement time is as follows: after the seawater is discharged, the change of carbon flux is measured within 15 minutes within half an hour.

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