Zedoary turmeric oil nanofiber membrane, preparation method and application thereof, and cotton sliver containing zedoary turmeric oil

Abstract

The invention belongs to the technical field of external preparations for skin, and particularly relates to a zedoary turmeric oil nanofiber membrane, a preparation method and application thereof and a cotton sliver containing zedoary turmeric oil. The preparation method comprises the following steps: mixing Tween-80 and zedoary turmeric oil to obtain an oil phase solution; mixing the polyethylene oxide aqueous solution and the oil phase solution to obtain a drug-containing emulsion; mixing the chitosan acid solution and the drug-containing emulsion to obtain a spinning solution; injecting the spinning solution into a microfluidic spinning device for spinning to obtain the zedoary turmeric oil nanofiber membrane. The problems that the cream is poor in stability and prone to layering or hardening are solved, and the problems that the cream is poor in air permeability and not suitable for wounds with seepage are solved. The invention provides a cotton sliver containing zedoary turmeric oil. The problem that the zedoary turmeric oil cannot be used in the menstrual period during medication is solved, and meanwhile the problems that medication compliance is poor and zedoary turmeric oil is prone to volatilization and storage can be solved through the shape, convenient to use, of the cotton sliver.

Description

Technical Field

[0001] This invention belongs to the field of topical skin preparation technology, specifically relating to a turmeric oil nanofiber membrane, its preparation method and application, and a tampon containing turmeric oil. Background Technology

[0002] Zedoary turmeric oil is a volatile oil extracted from the dried rhizome of *Curcuma zedoaria*, a plant in the ginger family, through steam distillation. As an important active ingredient in traditional Chinese medicine, its application history can be traced back to ancient texts such as the *Compendium of Materia Medica*. Modern pharmacological studies have shown that zedoary turmeric oil possesses multiple effects, including dispelling wind-heat, clearing meridians, reducing swelling, and relieving pain. Its mechanism of action is closely related to its more than twenty active ingredients, mainly including curzerene, germacrone, curdione, neocurdione, and the core component curcumol. Curcumol has been shown to exert anti-tumor effects through multiple pathways, such as inhibiting tumor cell proliferation cycles, inducing apoptosis, and regulating the immune microenvironment. It also exhibits antiviral and anti-inflammatory activities by interfering with viral replication cycles and inhibiting the release of inflammatory mediators, laying a scientific foundation for its widespread application in modern medicine.

[0003] Currently, the domestic turmeric oil preparation system includes eight dosage forms: creams, injections, eye drops, suppositories, soft capsules, and sprays, meeting different clinical needs. Topical preparations of turmeric oil are mainly suppositories and creams. Creams are frequently used as topical skin preparations. Creams have relatively poor stability and are easily affected by environmental factors such as temperature and humidity. In high-temperature environments, the oil and water phases of the cream may separate; while in low-temperature environments, the cream may harden, affecting its application and efficacy. Furthermore, its poor breathability means that while creams form a protective film on the skin, which aids in drug absorption, it can also hinder skin respiration and perspiration. Prolonged use may lead to discomfort such as stuffiness and itching, especially for oily skin or in hot and humid environments. Additionally, creams are not suitable for wounds with exudate, as they may hinder drainage, leading to increased local exudation and worsening of the condition. Suppositories are mainly used as vaginal preparations. However, the defects of turmeric oil suppositories as vaginal preparations are very obvious, including inconvenience in use and poor user compliance. The inconvenience in use often occurs during menstruation, as turmeric oil suppositories need to be avoided during menstruation. Moreover, the inconvenience in use often occurs in summer, as the high temperature in summer can easily cause the suppositories to deform, affecting the user experience.

[0004] In summary, the limited variety of topical preparations available for Curcuma zedoaria oil currently restricts its widespread application. Summary of the Invention

[0005] The purpose of this invention is to provide a turmeric oil nanofiber membrane, its preparation method and application, and a tampon containing turmeric oil. The turmeric oil nanofiber membrane prepared by this invention solves the problems of poor stability, easy layering or hardening of ointments, and also solves the problem of poor air permeability of ointments, making them unsuitable for wounds with exudate. In addition, this invention also solves the problem of not being able to use the medication during menstruation, and the easy-to-use shape of the tampon can improve poor medication compliance and the problem of easy evaporation and preservation of turmeric oil.

[0006] To achieve the above objectives, the present invention provides the following technical solution: This invention provides a method for preparing a turmeric oil nanofiber membrane, comprising the following steps: Tween-80 and Curcuma zedoaria oil were mixed to obtain an oil phase solution; An aqueous solution of polyethylene oxide and the oil phase solution are mixed to obtain a drug-containing emulsion; The chitosan acid solution and the drug-containing emulsion are mixed to obtain a spinning solution, wherein the chitosan content in the chitosan acid solution is <4% (w / v), and the volume ratio of the chitosan acid solution to the drug-containing emulsion is 1:1 to 1:2.5. The spinning solution is injected into a microfluidic spinning device to perform spinning, thereby obtaining the turmeric oil nanofiber membrane. The spinning propulsion speed is <3 mL / h.

[0007] Preferably, the volume ratio of Tween-80 to Curcuma oil is 0.25~1.5:1.

[0008] Preferably, the content of polyethylene oxide in the aqueous polyethylene oxide solution is 2.5-8% (w / v).

[0009] Preferably, the mass ratio of the polyethylene oxide to the amount of the turmeric oil in the polyethylene oxide aqueous solution is (0.5~1) g: 1 mL.

[0010] Preferably, the chitosan acid solution comprises chitosan and an aqueous acetic acid solution, wherein the chitosan content in the chitosan acid solution is 0.5-2% (w / v); and the acetic acid content in the aqueous acetic acid solution is 1-2% (w / v).

[0011] Preferably, the volume ratio of the chitosan acid solution to the drug-containing emulsion is 1:1 to 1:1.5.

[0012] Preferably, the spinning conditions include: a receiving distance of 15-25 cm, a pressure of 0.2-0.4 MPa, a receiver rotation speed of 400-500 rpm, and a feed rate of 1-3 mL / h, not exceeding 3 mL / h.

[0013] The present invention provides a turmeric oil nanofiber membrane prepared by the preparation method described in the above technical solution.

[0014] This invention provides the application of the turmeric oil nanofiber membrane described above in the preparation of turmeric oil topical skin preparations.

[0015] This invention provides a tampon containing turmeric oil, comprising a tampon body and cotton thread. The tampon body includes a tampon body body and an absorbent nonwoven fabric covering the tampon body body. The tampon body body includes the turmeric oil nanofiber membrane described in the above technical solution and a cotton-containing nonwoven fabric wrapped around the outside of the turmeric oil nanofiber membrane.

[0016] This invention provides a method for preparing a turmeric oil nanofiber membrane, comprising the following steps: mixing Tween-80 and turmeric oil to obtain an oil phase solution; mixing an aqueous solution of polyethylene oxide (PEO) with the oil phase solution to obtain a drug-containing emulsion; mixing a chitosan acid solution with the drug-containing emulsion to obtain a spinning solution, wherein the chitosan content in the chitosan acid solution is <4% (w / v), and the volume ratio of the chitosan acid solution to the drug-containing emulsion is 1:1 to 1:2.5; injecting the spinning solution into a microfluidic spinning device for spinning to obtain the turmeric oil nanofiber membrane, wherein the spinning propulsion speed is <3 mL / h. This invention addresses the problem of poor stability, easy delamination, or hardening of ointments by rationally designing the types and amounts of raw materials and using microfluidic spinning to form fibrous membranes from Curcuma zedoaria oil. Furthermore, by introducing chitosan and PEO into the Curcuma zedoaria oil fibrous membrane through microfluidic spinning, the resulting Curcuma zedoaria oil nanofiber membrane exhibits high porosity, thus solving the problem of poor air permeability in ointments, making them unsuitable for wounds with exudate.

[0017] This invention provides a turmeric oil nanofiber membrane with a three-dimensional nanofiber network structure obtained through microfluidic spinning. This three-dimensional nanofiber network has high specific surface area and porous structure, which can effectively simulate the natural extracellular matrix environment, providing an ideal biophysical microenvironment for cell adhesion, proliferation and migration, thereby significantly accelerating tissue regeneration and wound healing. At the same time, by optimizing the types and ratios of raw materials, this invention produces a turmeric oil nanofiber membrane with hydrophilicity and breathability, creating a moderately moist environment conducive to cell activity. Its dense nanofiber network can both block microbial invasion and reduce the risk of infection, and enable controlled release delivery of therapeutic drugs, ensuring a continuous therapeutic effect during the wound repair process.

[0018] This invention employs a microfluidic spinning method, which, compared to traditional membrane preparation processes, overcomes technical bottlenecks such as solvent residue in slurry film preparation, low yield in electrospinning, and mechanical defects in 3D printing. Production batch stability and continuous operation cycles are significantly superior to traditional processes, with reduced unit energy consumption. It is also easier to achieve continuous production, significantly improving safety and causing no environmental pollution, fully aligning with the concept of green production. This provides a breakthrough solution for the large-scale industrialization of biomedical membrane materials.

[0019] This invention provides a tampon containing turmeric oil, comprising a tampon body and cotton thread. The tampon body includes a tampon core and an absorbent nonwoven fabric covering the tampon core. The tampon core includes the turmeric oil nanofiber membrane described in the above-mentioned technical solution and a cotton-containing nonwoven fabric wrapped around the outside of the turmeric oil nanofiber membrane. This invention uses the turmeric oil nanofiber membrane as the core to obtain a turmeric oil-containing tampon product, which can solve the problem of not being able to use medication during menstruation. At the same time, the convenient shape of the tampon can improve poor medication adherence and address the problem of the volatile nature of turmeric oil and its preservation. Attached Figure Description

[0020] Figure 1 A photograph of the turmeric oil nanofiber membrane prepared in Example 1; Figure 2 Scanning electron microscope image of nanofibers in the turmeric oil nanofiber membrane prepared in Example 1; Figure 3 Transmission electron microscopy image of the nanofibers in the turmeric oil nanofiber membrane prepared in Example 1; Figure 4 The FTIR spectrum of the turmeric oil nanofiber membrane prepared in Example 1 is shown below. Figure 5 The image shows the in vitro release of the Curcuma zedoaria oil nanofiber membrane prepared in Example 1. Figure 6 The image shows the water contact angle of the turmeric oil nanofiber membrane prepared in Example 1. Detailed Implementation

[0021] This invention provides a method for preparing a turmeric oil nanofiber membrane, comprising the following steps: Tween-80 and Curcuma zedoaria oil were mixed to obtain an oil phase solution; An aqueous solution of polyethylene oxide and the oil phase solution are mixed to obtain a drug-containing emulsion; The chitosan acid solution and the drug-containing emulsion are mixed to obtain a spinning solution, wherein the chitosan content in the chitosan acid solution is <4% (w / v), and the volume ratio of the chitosan acid solution to the drug-containing emulsion is 1:1 to 1:2.5. The spinning solution is injected into a microfluidic spinning device to perform spinning, thereby obtaining the turmeric oil nanofiber membrane. The spinning propulsion speed is <3 mL / h.

[0022] In this invention, unless otherwise specified, all raw materials / components used in the preparation are commercially available products well known to those skilled in the art.

[0023] This invention involves mixing Tween-80 and Curcuma zedoaria oil to obtain an oil phase solution. In this invention, the volume ratio of Tween-80 to Curcuma zedoaria oil is preferably 0.25~1.5:1, more preferably 0.5~1.2:1, and in some examples, it can be 1:1. The mixing is preferably performed under ultrasonic conditions.

[0024] After obtaining the oil phase solution, the present invention mixes the aqueous solution of polyethylene oxide with the oil phase solution to obtain a drug-containing emulsion.

[0025] In this invention, the polyethylene oxide aqueous solution comprises polyethylene oxide and water, wherein the water may be purified water. The content of polyethylene oxide in the polyethylene oxide aqueous solution is preferably 2.5-8% (w / v), more preferably 3-6% (w / v), and in the examples, it may be 5% (w / v), that is, the preferred ratio of polyethylene oxide to water in the polyethylene oxide aqueous solution is (0.5-1) g: 20 mL. The preparation method of the polyethylene oxide aqueous solution preferably includes the following steps: dissolving the polyethylene oxide in water under stirring, and then allowing it to stand to remove bubbles, thereby obtaining the polyethylene oxide aqueous solution. The preferred ratio of the mass of polyethylene oxide to the amount of Curcuma zedoaria oil in the polyethylene oxide aqueous solution is (0.5-1) g: 1 mL, and in the examples, it may be 1 g: 1 mL.

[0026] In this invention, the preferred method for mixing the polyethylene oxide aqueous solution and the oil phase solution is to add the oil phase solution dropwise to the polyethylene oxide aqueous solution under stirring.

[0027] After obtaining the medicated emulsion, the present invention mixes the chitosan acid solution and the medicated emulsion to obtain a spinning solution, wherein the chitosan content in the chitosan acid solution is <4% (w / v) and the volume ratio of the chitosan acid solution to the medicated emulsion is >1:3.

[0028] In this invention, the chitosan acid solution preferably comprises chitosan and an aqueous acetic acid solution. The acetic acid content in the aqueous acetic acid solution is preferably 1-2% (w / v). The chitosan content in the chitosan acid solution is preferably 0.5-2% (w / v), more preferably 1-2% (w / v), and in the examples, it can be 2% (w / v). The volume ratio of the chitosan acid solution to the drug-containing emulsion is preferably 1:1 to 1:1.5, and in the examples, it can be 1:1, 1:1.25, or 1:1.5.

[0029] This invention selects chitosan as an excipient to obtain a chitosan-based nanofiber membrane that not only provides physical support similar to the extracellular matrix, but its degradation products can also promote tissue regeneration by regulating cell signaling pathways. The natural antibacterial properties of chitosan can help better regulate the flora in the vaginal and skin environments, achieving anti-inflammatory and antibacterial effects.

[0030] After obtaining the spinning solution, the present invention injects the spinning solution into a microfluidic spinning device to perform spinning, thereby obtaining the turmeric oil nanofiber membrane. The preferred spinning speed is <3 mL / h.

[0031] In this invention, the preferred spinning conditions include: a receiving distance of 15-25 cm; a pressure of 0.2-0.4 MPa, which in the embodiments can be 0.2 MPa, 0.3 MPa, or 0.4 MPa; a receiver rotation speed of 400-500 rpm; and a feed rate of 1-3 mL / h, but not 3 mL / h, which in the embodiments can be 1 mL / h.

[0032] The present invention provides a turmeric oil nanofiber membrane prepared by the preparation method described in the above technical solution.

[0033] This invention provides the application of the turmeric oil nanofiber membrane described above in the preparation of turmeric oil topical skin preparations.

[0034] This invention provides a tampon containing turmeric oil, comprising a tampon body and cotton thread. The tampon body includes a tampon body body and an absorbent nonwoven fabric covering the tampon body body. The tampon body body includes the turmeric oil nanofiber membrane described in the above technical solution and a cotton-containing nonwoven fabric wrapped around the outside of the turmeric oil nanofiber membrane.

[0035] The method for preparing the tampons containing turmeric oil provided by the present invention preferably includes: stacking the turmeric oil nanofiber membrane with a cotton-containing nonwoven fabric for making tampons, wherein the turmeric oil nanofiber membrane covers the interior of the cotton-containing nonwoven fabric, and the two are combined to form an absorbent body, the outer layer is covered with a water-absorbing nonwoven fabric and unwound, and the membrane is divided into tampons of appropriate size, which are then combined with cotton yarn and pressed into tampons.

[0036] In this invention, the tampons containing turmeric oil are used directly as sanitary tampons.

[0037] Alternatively, the tampons containing turmeric oil can be used as auxiliary tampons for vaginal administration. When used as auxiliary tampons for vaginal administration, the present invention uses the tampons containing turmeric oil as a base, and suppositories can be installed on the upper part.

[0038] To further illustrate the present invention, the technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0039] Example 1 prescription: 1 mL of Curcuma zedoaria oil; Tween-80 1 mL; Chitosan 0.4 g; PEO 1 g.

[0040] Preparation method: Oil phase solution: Take 1 mL of the prescribed amount of Tween-80 and 1 mL of Curcuma zedoaria oil, mix them, and then dissolve them by sonication to obtain the oil phase solution; PEO drug-containing solution: Dissolve 1g of the prescribed amount of PEO in 20 mL of pure water, stir to dissolve, and let stand to defoam, to obtain 20 mL of 5% (w / v) PEO solution. Add the oil phase solution dropwise to the PEO solution while stirring and stir until it is uniformly emulsion. Chitosan solution: Dissolve 0.4g of the prescribed amount of chitosan in 20 mL of 1% (w / v) glacial acetic acid aqueous solution, stir and mix evenly to obtain a 2% (w / v) chitosan solution, and let it stand to defoam or use ultrasound to defoam; A simple microfluidic spinning device was constructed by mixing chitosan solution and PEO drug-containing solution at a volume ratio of 1:1 and then drawing the mixture into a 10 mL syringe. The syringe and microfluidic chip were connected by a tubing. Spinning parameters were set as follows: receiver distance 15 cm, pressure 0.3 MPa, receiver rotation speed 400 rpm, and propulsion speed 1 mL / h, resulting in a Curcuma zedoaria oil nanofiber membrane.

[0041] The nanofiber membrane prepared in this embodiment can be used directly as a topical skin preparation.

[0042] The nanofiber membrane is superimposed on the cotton-containing nonwoven fabric used to make tampons. The nanofiber membrane is wrapped on the cotton-containing nonwoven fabric and the two are combined to form an absorbent body. The outer layer is covered with absorbent nonwoven fabric and unwound. It is then divided into tampons of appropriate size and combined with cotton yarn to press into tampons, or divided into smaller tampons as a base. A suppository can be installed on the top, which can be used as an auxiliary tampon for vaginal administration.

[0043] Example 2 prescription: 1 mL of Curcuma zedoaria oil; Tween-80 1 mL; Chitosan 0.4 g; PEO 1 g.

[0044] Preparation method: Oil phase solution: Take 1 mL of the prescribed amount of Tween-80 and 1 mL of Curcuma zedoaria oil, mix them, and then dissolve them by sonication to obtain the oil phase solution; PEO drug-containing solution: Dissolve 1g of the prescribed amount of PEO in 20 mL of pure water, stir to dissolve, and let stand to defoam to obtain a 5% (w / v) PEO solution. Add the oil phase solution dropwise to the PEO solution while stirring and stir until it is uniformly emulsion. Chitosan solution: Dissolve 0.4g of the prescribed amount of chitosan in 20 mL of 1% (w / v) glacial acetic acid aqueous solution, stir and mix evenly to obtain 20 mL of 2% (w / v) chitosan solution, let stand to defoam or use ultrasonic defoaming; A simple microfluidic spinning device was constructed by mixing chitosan solution and PEO drug-containing solution at a volume ratio of 1:1.25 and then drawing the mixture into a 10 mL syringe. The syringe and microfluidic chip were connected by a tubing. Spinning parameters were set as follows: receiver distance 15 cm, pressure 0.3 MPa, receiver rotation speed 400 rpm, and propulsion speed 1 mL / h, resulting in a Curcuma zedoaria oil nanofiber membrane.

[0045] The nanofiber membrane prepared in this embodiment can be used directly as a topical skin preparation.

[0046] The nanofiber membrane is superimposed on the cotton-containing nonwoven fabric used to make tampons. The nanofiber membrane is wrapped on the cotton-containing nonwoven fabric and the two are combined to form an absorbent body. The outer layer is covered with absorbent nonwoven fabric and unwound. It is then divided into tampons of appropriate size and combined with cotton yarn to press into tampons, or divided into smaller tampons as a base. A suppository can be installed on the top, which can be used as an auxiliary tampon for vaginal administration.

[0047] Example 3 prescription: 1 mL of Curcuma zedoaria oil; Tween-80 1 mL; Chitosan 0.4 g; PEO 1 g.

[0048] Preparation method: Oil phase solution: Take 1 mL of the prescribed amount of Tween-80 and 1 mL of Curcuma zedoaria oil, mix them, and then dissolve them by sonication to obtain the oil phase solution; PEO drug-containing solution: Dissolve 1g of the prescribed amount of PEO in 20 mL of pure water, stir to dissolve, and let stand to defoam to obtain a 5% (w / v) PEO solution. Add the oil phase solution dropwise to the PEO solution while stirring and stir until it is uniformly emulsion. Chitosan solution: Dissolve 0.4g of the prescribed amount of chitosan in 20 mL of 1% (w / v) glacial acetic acid aqueous solution, stir and mix evenly to obtain 20 mL of 2% (w / v) chitosan solution, let stand to defoam or use ultrasonic defoaming; A simple microfluidic spinning device was constructed by mixing chitosan solution and PEO drug-containing solution at a volume ratio of 1:1.5 and then drawing the mixture into a 10 mL syringe. The syringe and microfluidic chip were connected by a tubing. Spinning parameters were set as follows: receiver distance 15 cm, pressure 0.3 MPa, receiver rotation speed 400 rpm, and propulsion speed 1 mL / h, resulting in a Curcuma zedoaria oil nanofiber membrane.

[0049] The nanofiber membrane prepared in this embodiment can be used directly as a topical skin preparation.

[0050] The nanofiber membrane is superimposed on the cotton-containing nonwoven fabric used to make tampons. The nanofiber membrane is wrapped on the cotton-containing nonwoven fabric and the two are combined to form an absorbent body. The outer layer is covered with absorbent nonwoven fabric and unwound. It is then divided into tampons of appropriate size and combined with cotton yarn to press into tampons, or divided into smaller tampons as a base. A suppository can be installed on the top, which can be used as an auxiliary tampon for vaginal administration.

[0051] Figure 1 This is a photograph of the nanofiber membrane prepared in Example 1.

[0052] Depend on Figure 1 It can be seen that the turmeric oil emulsion nanofiber film prepared in Example 1 has a uniform thickness and a smooth appearance.

[0053] Figure 2 The image shown is a scanning electron microscope image of the nanofiber membrane prepared in Example 1.

[0054] Figure 2 SEM results showed that the turmeric oil emulsion nanofibers were uniform, unoriented, and had smooth surfaces without bead-like structures.

[0055] Figure 3 The image shown is a transmission electron microscope (TEM) image of the nanofiber membrane prepared in Example 1.

[0056] Depend on Figure 3 It can be seen that the nanofiber surface is smooth and has no bead-like structure, indicating that the drug-loaded nanofiber membrane prepared by physical stirring and microfluidic air-jet spinning technology can have the drug uniformly dispersed in the nanofiber membrane, which is beneficial to the uniform local drug concentration when applied externally.

[0057] Figure 4 The turmeric oil nanofiber membrane prepared for Example 1 (in) Figure 4 FTIR spectral scanning of the membrane (described as a composite membrane in the text).

[0058] Depend on Figure 4 It can be seen that the peak value generated by PEO is 2870 cm⁻¹. -1 (CH); 1460cm (CH2); 1145cm -1 1090cm -1 and 1055cm -1 The triple peak; 840cm -1 This is related to the helical structure of PEO. The FT-IR peak induced by chitosan is at 3290 cm⁻¹. -1 2875cm -1 1645cm -1 1540cm -1 1372cm -1 The values ​​are related to the OH, NH, -C=O, NH3, and -CH3 groups of the amide group, respectively. Furthermore, at 1025 cm⁻¹... -1 The peak at 2920 cm⁻¹ corresponds to a glycosidic bond. The peak in Curcuma zedoaria oil at 2920 cm⁻¹ corresponds to a glycosidic bond. -1 It is the CH stretching vibration of an aliphatic hydrocarbon, 1705 cm⁻¹ -1 The C=O stretching vibrations in turmeric ketone and turmeric alcohol, corresponding to the components of turmeric oil, occur at 1455 cm⁻¹. -1 and 1373cm -1 Corresponding to the bending vibrations of -CH2 and -CH3, there are many unspecified weak peaks distributed in the 1000-1450 cm⁻¹ range. -1 Corresponding to the terpenoids, the turmeric oil emulsion composite membrane contains all the characteristic peaks of PEO, CTS and turmeric oil, which fully demonstrates that turmeric oil emulsion nanofibers loaded with turmeric oil have been successfully prepared.

[0059] This invention investigates the drug loading and encapsulation efficiency of the turmeric oil nanofiber membrane prepared in Example 1. The absorbance values ​​were used to test the drug loading and encapsulation efficiency of the turmeric oil nanofiber membrane.

[0060] The drug loading of the turmeric oil nanofiber membrane prepared in Example 1 was 34.56±0.82%, and the encapsulation efficiency was 84.34±0.55%.

[0061] Figure 5The image shows the in vitro release of the nanofiber membrane prepared in Example 1. The in vitro release experiment was conducted as follows: W0 (9 mg) of Curcuma zedoaria oil technical grade and W0 (20 mg) of the Curcuma zedoaria oil nanofiber membrane prepared in Example 1 were weighed. The initial actual drug mass was W0. The membrane was immersed in a dialysis bag (molecular weight cutoff of 3.5 kDa) in a test tube containing 20 mL of PBS (0.01 M, pH 7.4) buffer solution. The tube was placed in a 37°C constant temperature shaker at 300 rpm. At 2, 4, 6, 8, 10, 12, 24, 48, 72, 96, 120, 144, and 168 h, 2 mL of the solution was taken out, and then 2 mL of PBS buffer solution was added. The absorbance was measured at 313 nm using a UV spectrophotometer, and the cumulative drug mass was calculated as Wt. The cumulative release rate was calculated using the following formula: Cumulative release rate (%) = Wt / W0 × 100%.

[0062] from Figure 5 In vitro release data of Curcuma zedoaria oil and Curcuma zedoaria oil emulsion nanofiber membranes lead to the conclusion that Curcuma zedoaria oil emulsion nanofiber membranes can delay the release of Curcuma zedoaria oil to a certain extent. The release rate is lower than that of Curcuma zedoaria oil in the early stage, but maintains a relatively stable release level in the later stage, exhibiting sustained-release characteristics. This is beneficial to prolong the action time of Curcuma zedoaria oil and optimize the efficacy.

[0063] Figure 6 The image shows the water contact angle of the nanofiber membrane prepared in Example 1.

[0064] The contact angle of nanofiber membranes reflects their hydrophilicity and affects cell adhesion and proliferation. Therefore, the hydrophilicity of the prepared nanofiber membranes was evaluated by measuring the contact angle. The results showed that the contact angles of the turmeric oil emulsion nanofiber membranes were 69.19° and 69.27°, respectively. Since the contact angles of both turmeric oil emulsion nanofiber membranes were below 90°, they exhibited good hydrophilicity, meeting the requirements for wound dressings.

[0065] Comparative Example 1 prescription: 1 mL of Curcuma zedoaria oil; Tween-80 1 mL; Chitosan 0.8 g; PEO 0.5 g.

[0066] Preparation method: Oil phase solution: Take 1 mL of the prescribed amount of Tween-80 and 1 mL of Curcuma zedoaria oil, mix them, and then dissolve them by sonication to obtain an oil phase solution; PEO drug-containing solution: Dissolve 0.5 g of the prescribed amount of PEO in 20 mL of pure water, stir to dissolve, and let stand to defoam, to obtain a 2.5% (w / v) PEO solution. Add the oil phase solution dropwise to the PEO solution while stirring and stir until it is homogeneous to form an emulsion. Chitosan solution: Dissolve 0.8 g of the prescribed amount of chitosan in 20 mL of 1% (w / v) glacial acetic acid aqueous solution, stir and mix evenly to obtain a 4% (w / v) chitosan solution, and let it stand to defoam or use ultrasound to defoam. A simple microfluidic spinning device was constructed by mixing chitosan solution and PEO drug-containing solution at a volume ratio of 1:1 and then drawing the mixture into a 10 mL syringe. The syringe and microfluidic chip were connected by a tubing. The spinning parameters were set as follows: receiver distance 15 cm, pressure 0.3 MPa, receiver rotation speed 400 rpm, and propulsion speed 1 mL / h, resulting in a nanofiber membrane.

[0067] Conclusion: Excessive chitosan solution concentration and viscosity led to clogging.

[0068] Comparative Example 2 prescription: 1 mL of Curcuma zedoaria oil; Tween-80 1 mL; Chitosan 0.4 g; PEO 1 g.

[0069] Preparation method: Oil phase solution: Take 1 mL of the prescribed amount of Tween-80 and 1 mL of Curcuma zedoaria oil, mix them, and then dissolve them by sonication to obtain an oil phase solution; PEO drug-containing solution: Dissolve 1 g of the prescribed amount of PEO in 20 mL of pure water, stir to dissolve, and let stand to defoam to obtain a 5% (w / v) PEO solution. Add the oil phase solution dropwise to the PEO solution while stirring and stir until it is uniformly emulsion. Chitosan solution: Dissolve 0.4 g of the prescribed amount of chitosan in 20 mL of 1% glacial acetic acid aqueous solution, stir and mix evenly to obtain a 2% (w / v) chitosan solution, and let it stand to defoam or use ultrasound to defoam; A simple microfluidic spinning device was constructed by mixing chitosan solution and PEO drug-containing solution at a volume ratio of 1:1 and then drawing the mixture into a 10 mL syringe. The syringe and microfluidic chip were connected by a tubing. The spinning parameters were set as follows: receiver distance 15 cm, pressure 0.3 MPa, receiver rotation speed 400 rpm, and feed rate 3 mL / h, resulting in a nanofiber membrane.

[0070] Conclusion: Excessive feed speed leads to excessive spinning liquid output, which can easily cause sticking.

[0071] Comparative Example 3 prescription: 1 mL of Curcuma zedoaria oil; Tween-80 1 mL; Chitosan 0.4 g; PEO 1 g.

[0072] Preparation method: Oil phase solution: Take 1 mL of the prescribed amount of Tween-80 and 1 mL of Curcuma zedoaria oil, mix them in equal amounts, and then dissolve them by sonication to obtain the oil phase solution; PEO drug-containing solution: Dissolve 1 g of the prescribed amount of PEO in 40 mL of pure water, stir to dissolve, and let stand to defoam, to obtain a 2.5% (w / v) PEO solution. Add the oil phase solution dropwise to the PEO solution while stirring and stir until it is homogeneous to form an emulsion. Chitosan solution: Dissolve 0.4g of the prescribed amount of chitosan in 40 mL of 1% (w / v) glacial acetic acid, stir and mix evenly to obtain a 1% (w / v) chitosan solution, and let it stand to defoam or use ultrasound to defoam. A simple microfluidic spinning device was constructed by mixing chitosan solution and PEO drug-containing solution at a volume ratio of 1:3, and then drawing the mixture into a 10 mL syringe. The syringe and microfluidic chip were connected by a tubing. Spinning parameters were then set. The nanofiber membrane was obtained by using a receiving distance of 15 cm, a pressure of 0.3 MPa, a receiver rotation speed of 400 rpm, and a propulsion speed of 1 mL / h.

[0073] Conclusion: The solution viscosity is too low to be spun.

[0074] As demonstrated by the above embodiments, this invention, through the rational design of the types and amounts of raw materials and the microfluidic spinning method to form fibrous membranes from Curcuma zedoaria oil, can solve the problems of poor stability, easy delamination, or hardening of ointments. Simultaneously, this invention introduces chitosan and PEO into the Curcuma zedoaria oil fibrous membrane using microfluidic spinning, resulting in a Curcuma zedoaria oil nanofiber membrane with high porosity, solving the problem of poor air permeability of ointments and their unsuitability for wounds with exudate. Furthermore, compared to traditional membrane preparation processes, this invention's microfluidic spinning method overcomes technical bottlenecks such as solvent residue in homogenization, low yield in electrospinning, and mechanical defects in 3D printing. The batch stability and continuous operation cycle are significantly better than traditional processes, with reduced unit energy consumption, easier continuous production, significantly improved safety, and no environmental pollution, fully conforming to the concept of green production. This provides a breakthrough solution for the large-scale industrialization of biomedical membrane materials.

[0075] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. Other embodiments can be obtained based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A method for preparing a turmeric oil nanofiber membrane, characterized in that, Includes the following steps: Tween-80 and Curcuma zedoaria oil were mixed to obtain an oil phase solution; An aqueous solution of polyethylene oxide and the oil phase solution are mixed to obtain a drug-containing emulsion; A chitosan acid solution and the drug-containing emulsion are mixed to obtain a spinning solution, wherein the chitosan content in the chitosan acid solution is <4% (w / v), and the volume ratio of the chitosan acid solution to the drug-containing emulsion is 1:1 to 1:2.

5. The spinning solution is injected into a microfluidic spinning device to perform spinning, thereby obtaining the turmeric oil nanofiber membrane. The spinning propulsion speed is <3 mL / h.

2. The preparation method according to claim 1, characterized in that, The volume ratio of Tween-80 to Curcuma zedoaria oil is 0.25~1.5:

1.

3. The preparation method according to claim 1, characterized in that, The content of polyethylene oxide in the aqueous solution is 2.5-8% (w / v).

4. The preparation method according to any one of claims 1 to 3, characterized in that, The mass ratio of the polyethylene oxide to the amount of the turmeric oil in the aqueous solution is (0.5~1) g: 1 mL.

5. The preparation method according to claim 1, characterized in that, The chitosan acid solution comprises chitosan and an aqueous acetic acid solution, wherein the chitosan content in the chitosan acid solution is 0.5-2% (w / v); and the acetic acid solution contains 1-2% (w / v) acetic acid.

6. The preparation method according to claim 1, characterized in that, The volume ratio of the chitosan acid solution to the drug-containing emulsion is 1:1 to 1:1.

5.

7. The preparation method according to claim 1, characterized in that, The spinning conditions include: a receiving distance of 15-25 cm, a pressure of 0.2-0.4 MPa, a receiver rotation speed of 400-500 rpm, and a feed rate of 1-3 mL / h, not exceeding 3 mL / h.

8. The turmeric oil nanofiber membrane prepared by the preparation method according to any one of claims 1 to 7.

9. The use of the turmeric oil nanofiber membrane according to claim 8 in the preparation of turmeric oil topical skin preparations.

10. A tampon containing turmeric oil, characterized in that, It includes a tampon body and cotton thread. The tampon body includes a tampon body body and an absorbent nonwoven fabric covering the tampon body body. The tampon body body includes the turmeric oil nanofiber membrane as described in claim 8 and a cotton-containing nonwoven fabric wrapped around the outside of the turmeric oil nanofiber membrane.

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