Nucleic acid extraction row pipe membrane sealing system

Abstract

The invention relates to the technical field of kit packaging, in particular to a nucleic acid extraction row pipe film sealing system which comprises a rotating disc device, a drying device, a film sealing machine, a slitting device, an overturning device, a detection conveying belt, a camera device, a transferring device, a row pipe support and an upper computer for controlling the film sealing system to work. By additionally arranging the turnover device, the detection conveying belt and the camera device and improving the row pipe and the row pipe support, automatic detection of unqualified liquid leakage products occurring in film sealing operation is achieved, production efficiency is improved, traditional manual detection is replaced, and then batch production and detection are achieved.

Description

Technical Field

[0001] This invention relates to the field of reagent kit packaging technology, and in particular to a nucleic acid extraction tube sealing system. Background Technology

[0002] Magnetic bead extraction of nucleic acids involves lysing the sample with a lysis buffer. The released nucleic acid molecules are specifically adsorbed onto the surface of magnetic beads, forming a magnetic bead-DNA polymer. Proteins and other impurities remain in the solution without being adsorbed. After a certain reaction time, a magnetic field is applied to separate the magnetic bead-DNA polymer from the liquid. The polymer is then recovered, and the sample is washed with a washing buffer to obtain pure DNA. Magnetic bead extraction eliminates the need for centrifugation and the addition of multiple reagents, making it simple to operate and meeting the requirements for automated nucleic acid extraction.

[0003] To adapt to the automated nucleic acid extraction of a fully automated nucleic acid extraction and purification instrument, a nucleic acid extraction manifold was designed. This manifold has lysis and elution tubes at both ends, and multiple washing tubes in the middle. Currently, nucleic acid extraction reagents are filled into the manifold by an automated filling system before leaving the factory, and then sealed with plastic or aluminum film using a sealing machine. After sealing, the manifold needs to be manually inverted to check for any liquid overflow, confirming whether the sealing is leaking. Any leaks need to be manually removed. Because manual quality inspection is inefficient, it reduces the efficiency of automated production. Therefore, a nucleic acid extraction manifold sealing system with automated quality inspection capability is proposed. Summary of the Invention

[0004] The purpose of this invention is to provide a nucleic acid extraction tube sealing system to solve the problems mentioned in the background art. The specific technical solution is as follows:

[0005] To achieve the above and other related objectives, this invention provides a nucleic acid extraction tube sealing system, comprising a turntable device, a drying device, a sealing machine, a cutting device, a flipping device, a detection conveyor belt, a camera device, a transfer device, a tube support, and a host computer for controlling the sealing system; wherein,

[0006] The upper side of the turntable device is provided with multiple limiting frames for placing the pipe support brackets. The multiple limiting frames are distributed in a circular matrix on the turntable device. A drying device, a sealing machine, a cutting device and a flipping device are arranged sequentially around the turntable device along the rotation direction. A detection conveyor belt is provided on one side of the flipping device. The flipping device is used to remove the pipe support brackets from the limiting frames and flip them upside down onto the detection conveyor belt. A camera device and a transfer device are respectively provided on both sides of the detection conveyor belt. The surface of the detection conveyor belt is provided with a water-sensitive color-changing layer.

[0007] The pipe support includes a support body for placing the pipes and a fastener for fixing the pipes.

[0008] As a preferred embodiment of the nucleic acid extraction tube sealing system of the present invention, a feeding conveyor belt and a loading robot are provided on one side of the turntable device. The loading robot is arranged between the drying device and the turning device. The loading robot is used to grab the tube support on the feeding conveyor belt and send it to the limiting frame.

[0009] As a preferred embodiment of the nucleic acid extraction tube sealing system of the present invention, the transfer device is provided with a defective product conveyor belt and a finished product conveyor belt on both sides. The transfer device is used to move the tube support on the detection conveyor belt to the defective product conveyor belt or the finished product conveyor belt.

[0010] As a preferred embodiment of the nucleic acid extraction and sealing tube system of the present invention, a blower is provided at the bottom of the detection conveyor belt.

[0011] As a preferred embodiment of the nucleic acid extraction tube sealing system of the present invention, the flipping device includes a support platform, columns, connecting rods, pneumatic clamps, a rotating shaft, and a drive motor; two columns are provided on the support platform, and connecting rods are rotatably mounted on the columns. The two connecting rods are fixedly connected to both ends of the rotating shaft. The drive motor is mounted on the support platform, and the output shaft of the drive motor is connected to the rotating shaft through a transmission mechanism. Through the transmission between the drive motor, the rotating shaft, and the connecting rods, the drive motor can drive the connecting rods to swing. Pneumatic clamps are provided on the connecting rods, and the two pneumatic clamps are used to clamp the tube support.

[0012] As a preferred embodiment of the nucleic acid extraction tube sealing system of the present invention, the fixing component is a clamping rod, the support body has four strip-shaped through holes, the clamping rod is slidably inserted into the strip-shaped through holes, and the tube has a groove that cooperates with the clamping rod.

[0013] As a preferred embodiment of the nucleic acid extraction tube sealing system of the present invention, a rubber protrusion for blocking the clamp is provided in the middle of the strip-shaped through hole.

[0014] The nucleic acid extraction tube sealing system provided by this invention has the following beneficial effects:

[0015] By adding a turning device, a detection conveyor belt, and a camera device, and by improving the manifold and manifold support, automatic detection of defective leaking products that occur during the sealing process has been achieved, which has improved production efficiency, replaced traditional manual inspection, and enabled mass production and inspection. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the structure of a nucleic acid extraction tube sealing system according to the present invention;

[0018] Figure 2 This is a three-dimensional structural diagram of the connecting pipe and connecting pipe support described in this invention;

[0019] Figure 3 yes Figure 1 A magnified view of a section at point A in the middle;

[0020] Figure 4 yes Figure 2 A magnified view of a section at point B. Detailed Implementation

[0021] The following detailed description, in conjunction with the accompanying drawings and specific embodiments, provides a further detailed explanation of the nucleic acid extraction and sealing system for multiple tubes proposed in this invention. The advantages and features of this invention will become clearer from the following description. It should be noted that the accompanying drawings are all in a very simplified form and use non-precise proportions, and are only used to facilitate and clarify the illustration of the embodiments of this invention.

[0022] In the description of this invention, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0023] It should be noted that the illustrations provided in the following embodiments are only schematic representations of the basic concept of the present invention. Therefore, the illustrations only show the components related to the present invention and are not drawn according to the actual number, shape and size of the components in the actual implementation. In the actual implementation, the shape, quantity and proportion of each component can be arbitrarily changed, and the layout of the components may also be more complex.

[0024] Therefore, the present invention provides a nucleic acid extraction tube sealing system, such as... Figure 1-4 As shown, the system includes a turntable device 1, a drying device 2, a sealing machine 3, a slitting device 4, a turning device 5, an inspection conveyor belt 6, a camera device 7, a transfer device 8, a connecting pipe support 9, and a host computer for controlling the sealing system; among which,

[0025] The upper side of the turntable device 1 is provided with multiple limiting frames 101 for placing the pipe support 9. The multiple limiting frames 101 are distributed in a circular matrix on the turntable device 1. The periphery of the turntable device 1 is arranged in sequence along the rotation direction with a drying device 2, a sealing machine 3, a cutting device 4, and a flipping device 5. A detection conveyor belt 6 is provided on one side of the flipping device 5. The flipping device 5 is used to remove the pipe support 9 from the limiting frames 101 and flip it upside down onto the detection conveyor belt 6. A camera device 7 and a transfer device 8 are respectively provided on both sides of the detection conveyor belt 6. A water-sensitive color-changing layer 601 is provided on the surface of the detection conveyor belt 6. The transfer device 8 is used to remove the pipe support 9 from the detection conveyor belt 6. The camera device 7 takes pictures of the water-sensitive color-changing layer 601 on the conveyor belt.

[0026] The pipe support 9 includes a support body 901 for placing the pipes 10 and a fixing member 902 for fixing the pipes 10 to prevent the pipes 10 from falling off the support body 901 when flipped.

[0027] To facilitate production, a feeding conveyor belt 11 and a loading robot 12 are provided on one side of the turntable device 1. The loading robot 12 is arranged between the drying device 2 and the turning device 5. The loading robot 12 is used to grab the pipe support 9 on the feeding conveyor belt 11 and send it to the limiting frame 101 to realize automatic loading.

[0028] Furthermore, the transfer device 8 is provided with a defective product conveyor belt 13 and a finished product conveyor belt 14 on both sides. The transfer device 8 is used to move the pipe support 9 on the inspection conveyor belt 6 onto the defective product conveyor belt 13 or the finished product conveyor belt 14.

[0029] During production, workers first install the manifold 10 onto the support body 901 and secure it to the support body 901 using fasteners 902. Then, it is fed into the automated filling system for filling. After filling, the manifold support 9 is moved by the feeding conveyor belt 11 to below the loading robot 12. The host computer controls the loading robot 12 to move the manifold support 9 onto the limiting frame 101. The turntable device 1 rotates, moving the manifold support 9 through the drying device 2. During filling, some liquid may spill onto the manifold. On the surfaces of the tubes 10 and the tube support 9, the drying device 2 dries the liquid on the surfaces of the tubes 10 and the tube support 9, and preheats the tubes 10 to facilitate subsequent sealing operations. The turntable device 1 continues to rotate, moving the dried tube support 9 below the sealing machine 3 for sealing. After sealing, the turntable device 1 rotates, moving the tube support 9 below the slitting device 4. To improve sealing efficiency, the multiple rows of tubes 10 on the tube support 9 are passed through a wider aluminum film or... The plastic film is packaged as a whole, and the slitting device 4 cuts the entire sealing film to separate the connecting tubes 10. The turntable device 1 drives the connecting tube support 9 to continue rotating to below the flipping device 5. The flipping device 5 operates, flipping the connecting tube support 9 and moving it onto the inspection conveyor belt 6. Since the connecting tubes 10 are fixed to the support body 901 by the fixing part 902, they will not slip during the flipping. After being inverted, the inspection conveyor belt 6 moves forward slowly. If there is liquid seepage inside the sealed connecting tubes 10, the liquid will seep onto the water-sensitive color-changing layer 601. This will cause the water-sensitive color-changing layer 601 to change color. After the connecting pipe support 9 moves to the transfer device 8, the transfer device 8 lifts the connecting pipe support 9. Then, the camera device 7 takes a picture of the water-sensitive color-changing layer 601 under the connecting pipe support 9 and sends it to the host computer. The host computer performs image recognition. When it recognizes that the water-sensitive color-changing layer 601 has changed color, it means that there is a problem with the sealing film of the connecting pipe 10 on the corresponding connecting pipe support 9. The host computer then controls the transfer device 8 to move the connecting pipe support 9 to the non-conforming product conveyor belt 13. Otherwise, it moves it to the finished product conveyor belt 14.

[0030] Furthermore, a blower is provided at the bottom of the detection conveyor belt 6. The blower is used to dry the moisture on the water-sensitive color-changing layer 601, so that the water-sensitive color-changing layer 601 returns to its original color.

[0031] Furthermore, the flipping device 5 includes a support platform 501, columns 502, connecting rods 503, a pneumatic chuck 504, a rotating shaft 505, and a drive motor 506. Two columns 502 are mounted on the support platform 501, and connecting rods 503 are rotatably mounted on the columns 502. The two connecting rods 503 are fixedly connected to both ends of the rotating shaft 505. The drive motor 506 is mounted on the support platform 501, and the output shaft of the drive motor 506 is connected to the rotating shaft 505 through a transmission mechanism. The drive motor 506, rotating shaft 505, and connecting rods 503 communicate with each other. The transmission enables the drive motor 506 to drive the connecting rod 503 to swing. The connecting rod 503 is equipped with two pneumatic chucks 504 for clamping the pipe support 9. During operation, the two pneumatic chucks 504 clamp the pipe support 9, and the drive motor 506 drives the rotating shaft 505 to rotate half a turn, so that the connecting rod 503 and the pneumatic chucks 504 drive the pipe support 9 to flip and move above the inspection conveyor belt 6. After the pneumatic chucks 504 release the pipe support 9, the pipe support 9 can be placed on the inspection conveyor belt 6.

[0032] Furthermore, the fixing component 902 is a locking rod, and the bracket body 901 has four strip-shaped through holes 903. The locking rod is slidably inserted into the strip-shaped through holes 903. The connecting pipe 10 has a locking groove 904 that cooperates with the locking rod. When fixing, the two locking rods can be manually moved so that the locking rods are locked into the locking groove 904. When it is necessary to remove the connecting pipe 10, the two locking rods can be moved away from the locking groove 904.

[0033] Furthermore, in order to fix the lever, a rubber protrusion 905 is provided in the middle of the strip-shaped through hole 903 to block the lever. When the lever is moved, the lever will compress the rubber protrusion 905 to generate resistance, thereby fixing the position of the lever.

[0034] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A nucleic acid extraction tube sealing system, characterized in that, It includes a turntable device, a drying device, a sealing machine, a slitting device, a turning device, an inspection conveyor belt, a camera device, a transfer device, a pipe support frame, and a host computer for controlling the sealing system; among which, The upper side of the turntable device is provided with multiple limiting frames for placing the pipe support brackets. The multiple limiting frames are distributed in a circular matrix on the turntable device. A drying device, a sealing machine, a cutting device and a flipping device are arranged sequentially around the turntable device along the rotation direction. A detection conveyor belt is provided on one side of the flipping device. The flipping device is used to remove the pipe support brackets from the limiting frames and flip them upside down onto the detection conveyor belt. A camera device and a transfer device are respectively provided on both sides of the detection conveyor belt. The surface of the detection conveyor belt is provided with a water-sensitive color-changing layer. The pipe support includes a support body for placing the pipes and a fastener for fixing the pipes.

2. The nucleic acid extraction tube sealing system according to claim 1, characterized in that, A feeding conveyor belt and a loading robot are provided on one side of the turntable device. The loading robot is arranged between the drying device and the turning device. The loading robot is used to grab the pipe support on the feeding conveyor belt and send it to the limiting frame.

3. The nucleic acid extraction tube sealing system according to claim 1, characterized in that, The transfer device is equipped with a non-conforming product conveyor belt and a finished product conveyor belt on both sides. The transfer device is used to move the pipe support on the inspection conveyor belt to the non-conforming product conveyor belt or the finished product conveyor belt.

4. The nucleic acid extraction tube sealing system according to claim 1, characterized in that, A blower is installed at the bottom of the inspection conveyor belt.

5. The nucleic acid extraction tube sealing system according to claim 1, characterized in that, The flipping device includes a support platform, columns, connecting rods, pneumatic chucks, a rotating shaft, and a drive motor. Two columns are mounted on the support platform, and connecting rods are rotatably mounted on the columns. The two connecting rods are fixedly connected to both ends of the rotating shaft. The drive motor is mounted on the support platform, and its output shaft is connected to the rotating shaft via a transmission mechanism. Through the transmission between the drive motor, the rotating shaft, and the connecting rods, the drive motor can drive the connecting rods to swing. Pneumatic chucks are mounted on the connecting rods, and the two pneumatic chucks are used to clamp the pipe support.

6. The nucleic acid extraction tube sealing system according to claim 5, characterized in that, The fixing component is a clamp rod. The bracket body has four strip-shaped through holes. The clamp rod is slidably inserted into the strip-shaped through holes. The connecting pipe has a groove that cooperates with the clamp rod.

7. The nucleic acid extraction tube sealing system according to claim 6, characterized in that, A rubber protrusion for blocking the lever is provided in the middle of the strip-shaped through hole.

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