Anti-oxidation and anti-aging composition, preparation method thereof and health-care product

Abstract

The invention provides an antioxidant and anti-aging composition as well as a preparation method and a health-care product thereof, and relates to the technical field of health-care products. The composition comprises the following raw materials: a sophora flower bud extract, a thyme extract, an echinacea purpurea extract, haematococcus pluvialis powder and vitamins. The effects of remarkably improving oxidation resistance and delaying senescence are achieved through specific components, the sophora flower bud extract, the thyme extract, the echinacea extract, the haematococcus pluvialis powder and the vitamins jointly support and interact, free radicals in the body are effectively removed, and meanwhile aging is delayed; the raw materials are natural, safe and healthy, and can supplement nutrition when being matched with vitamins.

Description

Technical Field

[0001] This invention relates to the field of health care product technology, specifically to an antioxidant and anti-aging composition, its preparation method, and health care products. Background Technology

[0002] Aging is a complex process influenced by factors including genetics, temperature, and dietary habits. Researchers in this field believe that the degenerative changes of aging are caused by the harmful effects of free radicals produced during normal cellular metabolism. The aging process in an organism is the result of the accumulation of free radicals continuously produced by the body's cells and tissues. Free radicals can cause DNA damage, lead to cell mutations, and induce tumor formation.

[0003] Specifically, free radicals are intermediate products of normal metabolism. They are highly reactive and can cause peroxidation reactions in various substances within cells, damaging cell membranes; they can also cross-link macromolecules such as proteins and nucleic acids, hindering their normal function. Various free radicals exist in living organisms, such as superoxide anion radicals (O2). -· Free radicals include hydroxyl radicals (OH·), nitrogen dioxide radicals (NO2·), and nitric oxide radicals (NO·). Free radicals are the main culprits of disease and aging. Once the body's ability to eliminate free radicals weakens, it will not only lead to aging, but also cause many diseases.

[0004] Studies have found that some diseases, such as cardiovascular and cerebrovascular diseases, central nervous system diseases, and cancer, are not solely caused by the oxidation of free radicals. Instead, the rapid combination of free radicals produces a substance with even greater oxidizing and cytotoxic properties than its parent molecule. Peroxynitrite, a product of the rapid combination of nitric oxide and superoxide anion free radicals, can mediate the modification and damage of biological macromolecules (DNA, proteins, and lipids). Researchers have studied the interaction between peroxynitrite and low-density lipoprotein (LDL), showing that peroxynitrite oxidizes LDL to ox-LDL (oxidized low-density lipoprotein). ox-LDL damages arterial endothelial cells, stimulates the proliferation of vascular smooth muscle cells, and leads to apoptosis of vascular cells, thus being closely linked to the pathogenesis of atherosclerosis.

[0005] Because the human body lacks specific enzymes to eliminate superoxide nitrite, the body's enzyme systems, such as SOD and CAT, have limited ability to scavenge free radicals generated by the body. When the body is affected by the environment, such as through injury or aging, a large number of free radicals, such as oxygen and hydroxyl radicals, are produced. At this time, the body's "protective enzymes" cannot completely eliminate the excess free radicals, requiring external supplementation to maximize the elimination of the toxic effects of free radicals.

[0006] Chinese patent application CN102132872A discloses a health food product composed of the following substances in weight percentages: 20-50% okra extract, 10-30% mandarin orange fruit extract, 10-20% sophora japonica flower bud extract, 5-20% honeysuckle extract, 5-20% green tea extract, 5-20% dendrobium officinale powder, and 5-20% American ginseng powder. This health food product, made from a combination of various natural plant extracts and precious traditional Chinese medicines, can effectively achieve a synergistic effect by acting on multiple target organs, exhibiting antioxidant, anti-aging, and free radical scavenging effects. Chinese patent application CN113599422A discloses a composition with anti-glycation effects. The raw materials, by weight, also include the following components: 1-50 parts apple polyphenols, 1-50 parts sophora japonica buds, 1-50 parts tea polysaccharides, 0.5-30 parts prickly pear, 1-50 parts ginseng, and 0.1-30 parts prebiotics. The composition exhibits significant inhibition of AGEs formation and extremely strong oxygen free radical scavenging ability, and has broad application prospects in the fields of anti-oxidation, anti-aging, and anti-diabetes.

[0007] The research progress on the anti-aging effects of traditional Chinese medicines that are both food and medicine (Chinese Journal of Pharmacology and Toxicology, October 2016, Vol. 30, No. 10, Xue Liying, Zhou Yuzhi, et al.) has published that the anti-aging activities of 52 kinds of traditional Chinese medicines that are both food and medicine have been investigated. Among them, those with more extensive anti-aging research include wolfberry, purslane, polygonatum, kudzu root, sea buckthorn, hawthorn, licorice, polygonatum, yam, and hemp seed. However, although chebula, sophora japonica, elsholtzia, nutmeg, amomum villosum, kelp, fennel, malt, lily, citron, sophora japonica buds, and houttuynia cordata have strong antioxidant activities, their anti-aging research has not yet been reported. At present, most of the anti-aging research on traditional Chinese medicines that are both food and medicine is based on a certain mechanism, and it is mostly concentrated on the free radical theory, while research on other mechanisms is relatively rare.

[0008] In summary, developing a composition with better antioxidant and anti-aging properties to meet market demands is a key research focus for researchers in this field. Summary of the Invention

[0009] To address the aforementioned problems, this invention provides an antioxidant and anti-aging composition that, under specific components, can significantly improve the effect of scavenging free radicals, provide better antioxidant protection, and delay aging.

[0010] To achieve the above objectives, the technical solution adopted by the present invention is as follows: On the one hand, the present invention provides an antioxidant and anti-aging composition comprising the following raw materials: Sophora japonica extract, thyme extract, echinacea extract, Haematococcus pluvialis powder, and vitamins.

[0011] Preferably, the mass ratio of the Sophora japonica extract, thyme extract, Echinacea extract and Haematococcus pluvialis powder is 6-8:4-6:4-6:5-7.

[0012] More preferably, the mass ratio of the Sophora japonica extract, thyme extract, Echinacea extract, and Haematococcus pluvialis powder is 7:5:5:6.

[0013] Preferably, the vitamin is selected from vitamin A acetate, vitamin C, vitamin E, vitamin B6, and vitamin B1. 12 At least one of them.

[0014] More preferably, the vitamins are vitamin A acetate, vitamin B6, and vitamin C.

[0015] Preferably, the mass ratio of vitamin A acetate, vitamin B6 and vitamin C is 8-12:2-4:5-8.

[0016] More preferably, the mass ratio of vitamin A acetate, vitamin B6 and vitamin C is 10:3:7.

[0017] Preferably, the preparation method of the Sophora japonica extract includes the following steps: Sophora japonica granules are pulverized and then extracted with alcohol.

[0018] More preferably, the preparation method of the Sophora japonica flower extract includes the following steps: crushing Sophora japonica flowers, passing them through a 40-80 mesh sieve, mixing them with a 70-80 wt% ethanol solution at a material-to-liquid ratio of 1 g: 12-18 L, ultrasonically extracting them at 45-50℃ for 0.5-1 h, filtering, and concentrating to obtain the extract.

[0019] Preferably, the power of the ultrasound is 400-600W. More preferably, the power of the ultrasound is 500W.

[0020] Preferably, the ingredients include the following raw materials by weight: 50-90 parts of Sophora japonica extract, 30-70 parts of thyme extract, 30-70 parts of Echinacea extract, 40-80 parts of Haematococcus pluvialis powder, and 10-30 parts of vitamins.

[0021] More preferably, the ingredients, by weight, include the following: 60-80 parts of Sophora japonica extract, 40-60 parts of thyme extract, 40-60 parts of Echinacea purpurea extract, 50-70 parts of Haematococcus pluvialis powder, and 15-25 parts of vitamins.

[0022] More preferably, by weight, it includes the following ingredients: 70 parts Sophora japonica extract, 50 parts thyme extract, 50 parts Echinacea extract, 60 parts Haematococcus pluvialis powder, and 20 parts vitamins.

[0023] Secondly, the present invention provides a method for preparing the antioxidant and anti-aging composition described above, comprising the following steps: mixing raw materials.

[0024] Thirdly, the present invention provides a health care product comprising the aforementioned antioxidant and anti-aging composition and a carrier acceptable in the field of health care products.

[0025] Preferably, the health product has the effects of anti-oxidation and anti-aging.

[0026] Preferably, the carrier includes, but is not limited to, excipients, buffers, emulsifiers, stabilizers, diluents, binders, preservatives, lubricants, pH adjusters, cryoprotectants, flavoring agents, fillers, and antioxidants.

[0027] Preferably, the excipient is selected from at least one of microcrystalline cellulose, lactose, pregelatinized starch, cyclodextrin, carboxymethyl cellulose, mannitol, magnesium stearate, starch, calcium phosphate, ethyl cellulose, methyl cellulose, alginate, gelatin, gum arabic, glyceryl monostearate, sodium glycolate starch glycolate, guar gum, glycerol, and propylene glycol.

[0028] Preferably, the buffer is selected from at least one of sodium dihydrogen phosphate, sodium bicarbonate, ammonium bicarbonate, sodium acetate, citrate, histidine, and succinate.

[0029] Preferably, the emulsifier is selected from at least one of magnesium stearate, zinc stearate, calcium stearate, glyceryl stearate, sorbitan isostearate, sorbitan oleate, and polyglycerol-3 polyricinoleate.

[0030] Preferably, the stabilizer is selected from at least one of farnesian gum, agar, alginate, cellulose ether, and carboxymethyl chitosan.

[0031] Preferably, the diluent is selected from at least one of erythritol, mannitol, sorbitol, xylitol, lactose, sucrose, corn starch, potato starch, calcium phosphate, calcium citrate, and crystalline cellulose.

[0032] Preferably, the adhesive is selected from at least one of ethanol, starch paste, pregelatinized starch, dextrin, syrup, hydroxypropyl methylcellulose, methylcellulose, sodium carboxymethylcellulose, ethylcellulose, polyvinyl alcohol, polyethylene glycol, sodium alginate, polyvinylpyrrolidone, gum arabic, gelatin, and alginic acid.

[0033] Preferably, the preservative is selected from at least one of methylparaben, propylparaben, methylparaben, ethylparaben, propylparaben, chlorobutanol, thimerosal, mercuric oxycyanide, phenoxyethanol, chlorhexidine, benzoic acid, sodium benzoate, chlorocresol, benzalkonium bromide, benzalkonium chloride, and ethylparaben.

[0034] Preferably, the lubricant is selected from at least one of magnesium stearate, zinc stearate, glyceryl monostearate, polyethylene glycol, stearic acid, talc, sodium chloride, sodium oleate, sodium lauryl sulfate, magnesium lauryl sulfate, sodium stearate fumarate, and poloxamer.

[0035] Preferably, the pH adjuster is selected from at least one of citric acid, fumaric acid, succinic acid, tartaric acid, malic acid, and ascorbic acid.

[0036] Preferably, the flavoring agent is selected from at least one of sweet orange flavoring, vanilla flavoring, strawberry flavoring, milk flavoring, banana flavoring, and cherry flavoring.

[0037] Preferably, the filler is selected from at least one of mannitol, xylitol, sorbitol, maltose, microcrystalline cellulose, glucose, lactose, sucrose, dextrin, starch, sodium alginate, and sodium bicarbonate.

[0038] Preferably, the antioxidant may be selected from at least one of L-cysteine ​​hydrochloride, L-cysteine ​​base, extracts rich in tocopherol (natural vitamin E), α-tocopherol (synthetic vitamin E), β-tocopherol, 6-tocopherol, propyl gallate, octyl gallate, dodecyl gallate, fumaric acid, malic acid, ascorbic acid (vitamin C), sodium ascorbate, calcium ascorbate, potassium ascorbate, ascorbate palmitate, and ascorbate stearate.

[0039] Preferably, the dosage form of the health care product includes capsules, tablets, granules, oral liquids, and powders.

[0040] More preferably, the dosage form of the health product is a capsule.

[0041] More preferably, the dosage form of the health product is a hard capsule.

[0042] Compared with the prior art, the present invention has the following beneficial effects: This invention achieves significant improvements in antioxidant properties and delays aging through specific components. Sophora japonica extract, thyme extract, echinacea extract, Haematococcus pluvialis powder, and vitamins work together to effectively eliminate free radicals in the body while delaying aging. The raw materials are all natural and safe, and when combined with vitamins, they can also supplement nutrition. Detailed Implementation

[0043] To make the technical means, creative features, achieved objectives, and effects of this invention readily understandable, the invention is further illustrated below with specific embodiments. However, these embodiments are merely preferred embodiments and not exhaustive. Other embodiments obtained by those skilled in the art based on the embodiments described herein without creative effort are all within the scope of protection of this invention. It is worth noting that the raw materials used in this invention are all common commercially available products, and their sources are not specifically limited. The technical and scientific terms used in the embodiments have meanings commonly understood by those skilled in the art to which this invention pertains.

[0044] Thyme extract: purchased from Jiahe, Shaanxi; Echinacea extract: purchased from Jiahe, Shaanxi; Haematococcus pluvialis powder: purchased from Shanghai Yuncaijin; The preparation method of Sophora japonica flower extract includes the following steps: washing, drying, pulverizing, and passing through a 60-mesh sieve, mixing with a 75wt% ethanol solution at a material-to-liquid ratio of 1g:15L, ultrasonically extracting at 48℃ for 45min, filtering, and concentrating to obtain the extract.

[0045] Example 1 An antioxidant and anti-aging composition, comprising, by weight parts: 70 parts Sophora japonica extract, 50 parts thyme extract, 50 parts Echinacea purpurea extract, 60 parts Haematococcus pluvialis powder, 10 parts vitamin A acetate, 3 parts vitamin B6, and 7 parts vitamin C.

[0046] The preparation method is as follows: mix the above raw materials evenly.

[0047] Example 2 An antioxidant and anti-aging composition, comprising, by weight parts: 60 parts Sophora japonica extract, 60 parts thyme extract, 60 parts Echinacea purpurea extract, 70 parts Haematococcus pluvialis powder, 8 parts vitamin A acetate, 2 parts vitamin B6, and 5 parts vitamin C.

[0048] The preparation method is as follows: mix the above raw materials evenly.

[0049] Example 3 An antioxidant and anti-aging composition, comprising, by weight parts: 80 parts Sophora japonica extract, 40 parts thyme extract, 40 parts Echinacea purpurea extract, 50 parts Haematococcus pluvialis powder, 12.5 parts vitamin A acetate, 4.2 parts vitamin B6, and 8.3 parts vitamin C.

[0050] The preparation method is as follows: mix the above raw materials evenly.

[0051] Comparative Example 1 An antioxidant and anti-aging composition, comprising, by weight parts: 40 parts Sophora japonica extract, 80 parts thyme extract, 20 parts Echinacea purpurea extract, 100 parts Haematococcus pluvialis powder, 1 part vitamin A acetate, 8 parts vitamin B6, and 1 part vitamin C.

[0052] The preparation method is as follows: mix the above raw materials evenly.

[0053] Comparative Example 2 The composition was prepared according to Example 1 of Chinese patent application CN102132872A.

[0054] Comparative Example 3 An antioxidant and anti-aging composition, compared with Example 1, replaces thyme extract, echinacea extract, and Haematococcus pluvialis powder with rosemary extract, astragalus extract, and resveratrol, respectively.

[0055] The product comprises the following ingredients by weight: 70 parts Sophora japonica extract, 50 parts rosemary extract, 50 parts Astragalus membranaceus extract, 60 parts resveratrol, 10 parts vitamin A acetate, 3 parts vitamin B6, and 7 parts vitamin C.

[0056] The preparation method is as follows: mix the above raw materials evenly.

[0057] Test Example 1 For superoxide anion radicals (O2) -· ), hydroxyl (OH·) radical and ABTS scavenging test Grouping: Blank control group: Deionized water Example group: 2.5g of the composition prepared in each example; Comparative example group: 2.5g of the composition prepared in each comparative example; Experimental method: Each group was brought to a final volume of 100 mL with deionized water and diluted 1000 times before use.

[0058] 1. Using the pyrogallol method: Under alkaline conditions, pyrogallol can undergo auto-oxidation to produce O2. -· Free radicals are generated simultaneously, producing colored intermediates with a maximum absorption peak at 320 nm. Different concentrations of the sample were added to the same reaction system (60 mmol / L pyrogallol solution, 10 mmol / L HCl, Tris-HCl buffer solution pH 8.2) in a 25°C constant temperature water bath, using deionized water as a reference. The absorbance was measured at 320 nm and calculated according to the formula: O2 -· Free radical scavenging rate % = (1-A) 样品 / A 对照 )×100; A样品 The absorbance of the sample tube, A 对照 : Absorbance of the control tube; Calculate the clearance rate.

[0059] 2. Using the Fenton reaction system model, the reaction of H2O2 and Fe... 2+ The mixture generates OH· free radicals, and salicylic acid reacts with OH· free radicals to produce a colored substance with an absorption peak at 510 nm. If an analyte with OH· scavenging function is added, it can compete with salicylic acid for OH·, thereby reducing the amount of colored product formed. Different concentrations of sample solutions were added to the reaction system (520 / mL salicylic acid solution, 1 mmol / L EDTA-Na2-Fe2+, 8.8 mmol / L H2O2, phosphate buffer solution pH=7.4), using deionized water as a reference. The absorbance was measured at 510 nm, and the OH· free radical scavenging rate was calculated according to Method 1.

[0060] The experimental results are shown in Table 1 below: Table 1

[0061] As shown in Table 1, in the in vitro antioxidant capacity test results, the free radical scavenging effect of the composition prepared in the embodiments of the present invention is significantly improved compared with the comparative example.

[0062] Test Example 2 (1) Experimental materials and equipment: The test sample was a composition prepared according to the present invention.

[0063] Multifunctional microplate reader (SynergyBiotech), centrifuge (Thermo), T-SOD test kit (100T / 96 samples, built in Nanjing).

[0064] (2) Experimental method: Eighty male Kunming mice aged 20-25g for 3 months were randomly divided into blank control group, model control group, Example 1-Example 3 group, and Comparative Example 1-Comparative Example 3 group, with 10 mice in each group. They were housed in separate cages and allowed free access to water and food.

[0065] First, a mouse model of D-galactose-induced aging was established. Except for the blank control group, mice in all other groups were subcutaneously injected with D-galactose (150 mg / kg) in the neck and back. The blank control group was subcutaneously injected with the same volume of physiological saline. This was done once daily for 6 weeks. Simultaneously with model establishment, groups 1-3 and Comparative Examples 1-3 were administered their respective prepared compositions (1.20 g / kg, 5% CMC-Na solution as solvent) by gavage. The blank control group and the model group were administered the same volume of 5% CMC-Na solution by gavage once daily for 6 weeks.

[0066] Mice were euthanized by vertebral dislocation, and orbital blood was collected. After standing for 1 hour, the blood was centrifuged (3000 rpm, 15 min) and the supernatant was frozen for later use. The activity of superoxide dismutase (T-SOD) in the mice was measured.

[0067] To calculate organ indices, the brain, liver, and thymus were removed separately. Surrounding adhesions and connective tissue were carefully removed, and the organs were placed on filter paper to absorb excess moisture. They were then precisely weighed using an analytical balance, and the indices of each organ were calculated. Organ index (mg / g) = organ weight (mg) / mouse body weight (g); Statistical analysis was performed using SPSS 27.0 software. Experimental results are expressed as mean ± standard deviation (X ± SD). One-way ANOVA was used for testing, and pairwise comparisons were performed using the LSD-t method. P < 0.05 and P < 0.01 were used to indicate significant and highly significant differences, respectively.

[0068] The test results are shown in Tables 2 and 3 below: Table 2

[0069] Note: & Compared with the blank control group, P<0.05; && This means P < 0.01.

[0070] # Compared with the model control group, P<0.05; ## This means P < 0.01.

[0071] % Compared with Example 1, P < 0.05; %% This means P < 0.01.

[0072] As shown in Table 2, compared with the blank control group, the T-SOD activity of the model control group was significantly reduced (P<0.05), indicating that the anti-aging model was successfully established. Compared with the model control group, the T-SOD activity of Examples 1-3 and Comparative Examples 1-3 was significantly increased (P<0.01 or P<0.05), and compared with Example 1, the T-SOD activity of Comparative Examples 1-3 was significantly reduced (P<0.01 or P<0.05).

[0073] Table 3

[0074] Note: & Compared with the blank control group, P<0.05; &&This means P < 0.01.

[0075] # Compared with the model control group, P<0.05; ## This means P < 0.01.

[0076] % Compared with Example 1, P < 0.05; %% This means P < 0.01.

[0077] As shown in Table 3, D-galactose can significantly reduce the organ indices of the brain, liver, and thymus in mice. In contrast, the brain, liver, and thymus indices of aging mice treated with the combination increased to varying degrees, indicating that the D-galactose-induced aging model mice have a protective effect and can effectively delay organ aging in mice.

[0078] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. An antioxidant, anti-aging composition, characterized in that, The antioxidant and anti-aging composition comprises the following raw materials: sophora flower extract, thyme extract, echinacea extract, haematococcus pluvialis powder and vitamins.

2. The composition of claim 1, wherein, The mass ratio of the sophora flower extract, the thyme extract, the echinacea extract and the haematococcus pluvialis powder is 6-8:4-6:4-6:5-7.

3. The composition of claim 1, wherein, The vitamin is selected from at least one of vitamin A acetate, vitamin C, vitamin E, vitamin B6, vitamin B 12 12 4. The composition of claim 3, wherein, The vitamins are vitamin A acetate, vitamin B6 and vitamin C with a mass ratio of 8-12:2-4:5-8.

5. The composition of claim 1, wherein, The preparation method of the sophora flower extract comprises the following steps: alcohol extraction after sophora flower is crushed.

6. The composition of claim 5, wherein, The preparation method of the sophora flower extract comprises the following steps: sophora flower is crushed, filtered through a 40-80 mesh sieve, mixed with 70-80 wt% ethanol solution at a solid-liquid ratio of 1g:12-18L, ultrasonically extracted at 45-50℃ for 0.5-1h, filtered and concentrated.

7. The composition of claim 1, wherein, The antioxidant and anti-aging composition comprises the following raw materials: sophora flower extract 50-90 parts, thyme extract 30-70 parts, echinacea extract 30-70 parts, haematococcus pluvialis powder 40-80 parts and vitamins 10-30 parts.

8. A process for the preparation of the antioxidant, anti-aging composition according to any one of claims 1 to 7, characterized in that, The method comprises the following steps: mixing the raw materials.

9. A health care product, characterized by, The antioxidant and anti-aging composition comprises the following raw materials: sophora flower extract 50-90 parts, thyme extract 30-70 parts, echinacea extract 30-70 parts, haematococcus pluvialis powder 40-80 parts and vitamins 10-30 parts.

10. The health care product of claim 9, wherein, The method comprises the following steps: mixing the raw materials. The antioxidant and anti-aging composition comprises the following raw materials: sophora flower extract 50-90 parts, thyme extract 30-70 parts, echinacea extract 30-70 parts, haematococcus pluvialis powder 40-80 parts and vitamins 10-30 parts. The method comprises the following steps: mixing the raw materials. The antioxidant and anti-aging composition comprises the following raw materials: sophora flower extract 50-90 parts, thyme extract 30-70 parts, echinacea extract 30-70 parts, haematococcus pluvialis powder 40-80 parts and vitamins 10-30 parts. The method comprises the following steps: mixing the raw materials.

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