Ginger-shaped notoginsenoside R1-metal ion self-assembled hydrogel and preparation method thereof

Abstract

The invention relates to a gingerous notoginsenoside R1-metal ion self-assembled hydrogel and a preparation method thereof. The self-assembled hydrogel comprises gingerous notoginsenoside R1, metal ions and a solvent, wherein the concentration of the notoginsenoside R1 and the concentration of the metal ions are both 1-100 mmol / L; the metal ions comprise at least one of divalent metal ions and trivalent metal ions. According to the invention, divalent metal ions or trivalent metal ions are used for generating coordination, so that the gingerous notoginsenoside R1 forms the self-assembled hydrogel, and a thickening agent does not need to be added; in the obtained hydrogel, metal ions are distributed in the gingerous notoginsenoside R1, and the gingerous notoginsenoside R1 can reach a relatively high concentration; meanwhile, the self-assembled hydrogel has excellent stability, and can still maintain the gel form after being inverted for 48 hours; the preparation process is simple, the preparation of the self-assembled hydrogel can be completed without heating, and the preparation method is suitable for industrial production.

Description

Technical Field

[0001] This invention relates to the field of supramolecular self-assembly technology, specifically to a ginger-like notoginsenoside R1-metal ion self-assembled hydrogel and its preparation method. Background Technology

[0002] Hydrogels, with their unique three-dimensional network structure, can adsorb and retain large amounts of water while maintaining a stable morphology, showing great application potential in drug delivery, tissue engineering, biomedical detection, and wound repair. Self-assembled hydrogels spontaneously form through non-covalent intermolecular interactions (such as hydrogen bonds, electrostatic attraction, and hydrophobic interactions), and the preparation process is simple and mild. They also possess good biocompatibility and biodegradability, meeting the stringent material requirements of modern biomedicine.

[0003] Ginger-like notoginsenoside R1, with the molecular formula C 42 H 66 O 14 With a molecular weight of 794.98, it is a natural triterpenoid saponin that has been proven to have various biological activities such as anti-tumor, anti-angiogenesis, and anti-inflammation.

[0004] However, notoginsenoside R1 has low solubility in water (it is difficult to dissolve in water at room temperature), which prevents it from reaching a high concentration, thus limiting its bioavailability and seriously hindering its practical application. At the same time, the existing technology for forming hydrogels by self-assembly of notoginsenoside R1 usually requires the addition of thickeners such as carbomer, or dispersion in a certain concentration of PBS solution followed by heating, which are quite demanding preparation conditions. Summary of the Invention

[0005] The purpose of this invention is to overcome the above-mentioned technical deficiencies and provide a ginger-like notoginsenoside R1-metal ion self-assembled hydrogel and its preparation method, solving the technical problems of low solubility of ginger-like notoginsenoside R1 leading to limited bioavailability and difficulty in self-assembling ginger-like notoginsenoside R1 to form a hydrogel in the prior art.

[0006] To achieve the above-mentioned technical objectives, the technical solution provided by this invention is as follows: In a first aspect, the present invention provides a ginger-like notoginsenoside R1-metal ion self-assembled hydrogel, comprising ginger-like notoginsenoside R1, metal ions and solvent; wherein the concentrations of ginger-like notoginsenoside R1 and metal ions are both 1 to 100 mmol / L; the metal ions include at least one of divalent metal ions and trivalent metal ions.

[0007] Secondly, the present invention provides a method for preparing the above-mentioned ginger-like notoginsenoside R1-metal ion self-assembled hydrogel, comprising the following steps: mixing ginger-like notoginsenoside R1 solution with soluble metal source solution evenly, and allowing it to stand at 10-30°C to form a self-assembled hydrogel.

[0008] Compared with the prior art, the beneficial effects of the present invention include: This invention utilizes the coordination effect of divalent or trivalent metal ions to induce the formation of a self-assembled hydrogel of notoginsenoside R1, eliminating the need for external thickeners. In the resulting hydrogel, metal ions are distributed within the notoginsenoside R1, achieving a high concentration of notoginsenoside R1. Furthermore, this self-assembled hydrogel exhibits excellent stability, maintaining its gel morphology even after being inverted for 48 hours. The preparation process of this invention is simple, requiring no heating to complete the preparation of the self-assembled hydrogel, making it suitable for industrial production. Attached Figure Description

[0009] Figure 1 This is a schematic diagram of the self-assembled hydrogel of ginger-like notoginsenoside R1 and metal ions in this invention and its preparation method. Figure 2 The self-assembled hydrogel of ginger-like notoginsenoside R1 and divalent copper ions prepared in Example 1 of this invention; Figure 3 The self-assembled hydrogel of ginger-like notoginsenoside R1 and divalent zinc ions prepared in Example 2 of this invention; Figure 4 The self-assembled hydrogel of ginger-like notoginsenoside R1 and divalent strontium ions prepared in Example 3 of this invention; Figure 5 The self-assembled hydrogel of ginger-like notoginsenoside R1 and ferric ions prepared in Example 4 of this invention; Figure 6 The self-assembled hydrogel of ginger-like notoginsenoside R1 and trivalent indium ions prepared in Example 5 of this invention; Figure 7 The self-assembled hydrogel of ginger-like Panax notoginseng saponin R1 and trivalent gallium ions prepared in Example 6 of this invention; Figure 8 The self-assembled hydrogels of ginger-like notoginsenoside R1 and different concentrations of ferric ions prepared in this invention; Figure 9 This is an aqueous solution of pure ginger-like notoginsenoside R1 prepared in Comparative Example 1 of the present invention. Detailed Implementation

[0010] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0011] To address the limitations of notoginsenoside R1 in terms of low solubility leading to limited bioavailability and the difficulty of it self-assembling into hydrogels, this invention provides a notoginsenoside R1-metal ion self-assembled hydrogel and its preparation method. The hydrogel is formed by combining notoginsenoside R1 with a series of metal ions (copper, zinc, calcium, strontium, magnesium, gold, iron, indium, and gallium). This series of hydrogels exhibits good stability, biocompatibility, and targeted biological activity, and the preparation process is simple and easy to operate, making it suitable for large-scale production.

[0012] Metal ions play key roles in organisms: copper ions have antibacterial and antioxidant properties; zinc ions participate in enzyme activity regulation, promoting cell growth, immune function, and wound healing; calcium ions regulate cell signal transduction, maintain neuromuscular function, and promote bone development; strontium ions improve bone metabolism and promote bone growth; magnesium ions promote cell proliferation, tissue regeneration, regulate the inflammatory microenvironment, and enhance antibacterial activity; gold ions mainly function in drug delivery, cancer treatment (such as photothermal therapy and photodynamic therapy), and bioimaging, and also have certain antibacterial activity; iron ions are essential elements in organisms, playing a central role in oxygen transport, energy metabolism, and immune function; indium ion complexes have wide applications in diagnostic imaging (SPECT, PET), antibacterial, anticancer, and photodynamic chemotherapy; gallium ions, due to their iron-mimicking behavior, show significant potential in antibacterial, anticancer, anti-inflammatory, orthopedic implant materials, and diagnostic imaging. By combining ginger-like notoginsenoside R1 with the aforementioned metal ions through self-assembly technology to form a hydrogel, it is expected to simultaneously improve the solubility and bioavailability of ginger-like notoginsenoside R1, while endowing the hydrogel with synergistic bioactivity, thus meeting the diverse needs of the biomedical field.

[0013] Currently, there are no publicly reported systematic studies on the formation of self-assembled hydrogels by notoginsenoside R1 with copper, zinc, calcium, strontium, magnesium, gold, iron, indium, and gallium ions. This invention develops a simple and efficient preparation process, which is of great significance for promoting basic scientific research and the transformation and application of biomedical technology.

[0014] In a first aspect, the present invention provides a ginger-like notoginsenoside R1-metal ion self-assembled hydrogel, comprising ginger-like notoginsenoside R1, metal ions and solvent; wherein the concentrations of ginger-like notoginsenoside R1 and metal ions are both 1 to 100 mmol / L; the metal ions include at least one of divalent metal ions and trivalent metal ions.

[0015] This invention enables notoginsenoside R1 to form a self-assembled hydrogel through coordination with divalent or trivalent metal ions, while monovalent metal ions such as sodium ions do not have this effect.

[0016] Preferably, the concentration of ginger-like notoginsenoside R1 is 5–50 mmol / L; at this concentration, the overall performance of the hydrogel is optimal.

[0017] Preferably, the concentration of divalent metal ions is 10–40 mmol / L; the concentration of trivalent metal ions is 5–20 mmol / L. Excessively high concentrations of any metal ion can lead to cytotoxicity, reducing its biomedical application value and increasing costs; while excessively low concentrations cannot promote the formation of self-assembled hydrogels.

[0018] Preferably, the divalent metal ions include at least one of copper ions, zinc ions, calcium ions, strontium ions, and magnesium ions; the trivalent metal ions include at least one of gold ions, iron ions, indium ions, and gallium ions.

[0019] Preferably, the solvent includes at least one of water, ethanol solution, and buffer solution with pH value of 6 to 8; wherein the buffer solution is most effective when pH=7.4.

[0020] Secondly, the present invention provides a method for preparing the above-mentioned ginger-like notoginsenoside R1-metal ion self-assembled hydrogel, comprising the following steps: The solution of ginger-like ginsenoside R1 was mixed evenly with a soluble metal source solution and allowed to stand at 10–30°C to form a self-assembled hydrogel.

[0021] See Figure 1 In the preparation method of the present invention, ginger-like notoginsenoside R1 and soluble metal source are dissolved in the same or different solvents, and then mixed evenly in a predetermined ratio to ensure that the two are fully and evenly contacted. After standing at room temperature, ginger-like notoginsenoside R1 and metal ions spontaneously assemble through intermolecular interactions, and finally form a structurally stable self-assembled hydrogel.

[0022] Preferably, the ginger-like notoginseng saponin R1 solution is prepared by adding ginger-like notoginseng saponin R1 to a first solvent and stirring until homogeneous; the soluble metal source solution is prepared by dissolving a soluble metal source in a second solvent; the first solvent and the second solvent are each independently selected from at least one of water, ethanol solution, and buffer solution with a pH value of 6 to 8.

[0023] Preferably, the soluble metal source includes at least one of soluble copper salt, soluble zinc salt, soluble calcium salt, soluble strontium salt, soluble magnesium salt, soluble auronic acid and gold salt, soluble iron salt, soluble indium salt, and soluble gallium salt.

[0024] Further preferably, the soluble copper salt is selected from one or more of copper sulfate, copper chloride, and copper acetate; the soluble zinc salt is selected from one or more of zinc nitrate, zinc chloride, and zinc acetate; the soluble calcium salt is selected from one or more of calcium chloride, calcium nitrate, and calcium acetate; the soluble strontium salt is selected from one or more of strontium nitrate, strontium chloride, and strontium acetate; the soluble magnesium salt is selected from one or more of magnesium chloride, magnesium nitrate, and magnesium phosphate; the soluble auric acid and gold salt are selected from one or more of chloroauric acid, gold chloride, sodium chloroaurate, potassium chloroaurate, and gold nitrate; the soluble iron salt is selected from one or more of ferric chloride, ferric nitrate, and ferric sulfate; the soluble indium salt is selected from one or more of indium chloride, indium nitrate, and indium sulfate; and the soluble gallium salt is selected from one or more of gallium chloride, gallium nitrate, and gallium sulfate.

[0025] Preferably, the ginger-like notoginsenoside R1 solution is mixed with an equal volume of soluble metal source solution.

[0026] Preferably, the temperature for standing is room temperature (20-25°C).

[0027] Preferably, the settling time is 1 to 1440 minutes.

[0028] A further preferred setting time is 5–60 minutes.

[0029] The main advantages of this invention are: (1) Improve bioavailability: The hydrogel formed by the self-assembly of the present invention improves the solubility and stability of ginger-like saponin R1 in water, greatly improving its bioavailability and opening up new avenues for its wide application in the biomedical field. (2) Simple preparation process: The preparation method of the present invention is simple, without the need for complex equipment and special processes. The operation process is simple and easy to understand and control, which is suitable for large-scale industrial production and lays a solid foundation for the commercial promotion of a series of hydrogels. (3) Flexible component control: The physicochemical properties and biological activity of hydrogel can be precisely controlled by adjusting the concentration ratio of ginger-like notoginsenoside R1 with different divalent or trivalent metal ions, adapting to diverse application scenarios.

[0030] Furthermore, the types of hydrogels obtained by this invention are extensive: Copper ion hydrogel: The anti-inflammatory and anti-tumor activities of ginger-like saponin R1 and the antibacterial and antioxidant properties of copper ions work synergistically to endow the hydrogel with excellent broad-spectrum antibacterial and anti-inflammatory regulation capabilities, making it suitable for the treatment of infected wounds and antibacterial dressings.

[0031] Zinc ion hydrogel: The two work synergistically to enhance cell proliferation and tissue repair activity. The wound healing promoting effect of zinc ions combined with the anti-inflammatory effect of ginger-like notoginsenoside R1 can accelerate skin wound repair and tissue regeneration, making it suitable for chronic wound care and tissue engineering scaffolds.

[0032] Calcium ion hydrogels: Relying on the biocompatibility and cell signaling regulation function of calcium ions, hydrogels have high biocompatibility and work synergistically with notoginsenoside R1 to maintain the stability of the tissue microenvironment, making them suitable for drug sustained-release carriers and biomedical detection platforms.

[0033] Strontium ion hydrogel: The bone metabolism-improving effect of strontium ions, combined with the anti-inflammatory activity of ginger-like saponin R1, can promote bone tissue repair and regeneration, making it suitable for the fields of bone defect repair and orthopedic implant auxiliary materials.

[0034] Magnesium ion hydrogel: The anti-inflammatory activity of ginger-like ginsenoside R1 and the regulatory and proliferative functions of magnesium ion energy metabolism can work synergistically to accelerate the repair of damaged cells and inhibit inflammation. It has a strong anti-inflammatory and wound-healing ability and is suitable for the field of acute wound repair and skin regeneration dressings.

[0035] Gold ion hydrogel: The anti-inflammatory and anti-tumor activities of ginger-like saponin R1 are synergistic with the drug delivery, tumor phototherapy (photothermal / photodynamic) and antibacterial capabilities of gold ions, enabling integrated diagnosis and treatment. It is suitable for the fields of precision tumor treatment and antibacterial drug carrier.

[0036] Iron ion hydrogel: The anti-inflammatory and anti-tumor activities of ginger-like notoginsenoside R1 synergistically work with iron ions to regulate oxygen transport, energy metabolism and immune function, thereby improving biocompatibility. It is suitable for adjuvant cancer treatment and repair of damaged tissues that require improved energy metabolism.

[0037] Indium ion hydrogel: Combining the anti-inflammatory and anti-cancer activities of ginger-like ginsenoside R1 with indium ion diagnostic imaging (SPECT / PET), antibacterial and photodynamic chemotherapy functions, it achieves precise diagnosis and treatment, and is suitable for integrated scenarios where tumor diagnosis and treatment are carried out simultaneously.

[0038] Gallium ion hydrogel: The anti-inflammatory activity of ginger-like ginsenoside R1 synergizes with the antibacterial and anticancer capabilities of gallium ions mimicking iron, as well as its compatibility with orthopedic implants, while taking into account biosafety, making it suitable for orthopedic infection repair and adjuvant tumor treatment.

[0039] The present invention will be further described in detail below through specific embodiments.

[0040] Example 1 The preparation method of the self-assembled hydrogel of ginger-like notoginsenoside R1 and copper ions includes the following steps: (1) Preparation of ginger-like saponin R1 solution: Accurately weigh 43 mg of ginger-like saponin R1, add it to 3.6 mL of deionized water, and sonicate for 30 minutes to obtain a ginger-like saponin R1 solution with a concentration of 15 mmol / L; (2) Preparation of copper ion solution: Weigh 100 mg of copper sulfate pentahydrate, add it to 10 mL of deionized water, stir for 5 minutes until completely dissolved, and obtain a copper ion solution with a concentration of 40 mmol / L; (3) Preparation of self-assembled hydrogel: Mix the two solutions of the above with equal volumes, stir at 180 r / min for 3 minutes, and let stand at room temperature for 10 minutes to obtain a light blue ginger-like notoginseng saponin R1-copper ion hydrogel (e.g. Figure 2 (As shown).

[0041] Example 2 The preparation method of the self-assembled hydrogel of ginger-like notoginsenoside R1 and zinc ions includes the following steps: (1) Preparation of ginger-like saponin R1 solution: Accurately weigh 43 mg of ginger-like saponin R1, add it to 3.6 mL of deionized water, and sonicate for 30 minutes to obtain a ginger-like saponin R1 solution with a concentration of 15 mmol / L; (2) Preparation of zinc ion solution: Weigh 55 mg of zinc chloride and add it to 10 mL of phosphate buffer solution with pH=7.4. Stir for 20 minutes to obtain a zinc ion solution with a concentration of 40 mmol / L. (3) Preparation of self-assembled hydrogel: Mix the two solutions of the above with equal volumes, stir at 180 r / min for 3 minutes, and let stand at room temperature for 10 minutes to obtain a white ginger-like notoginseng saponin R1-zinc ion hydrogel (e.g. Figure 3 (As shown).

[0042] Example 3 The preparation method of the self-assembled hydrogel of ginger-like notoginsenoside R1 and strontium ions includes the following steps: (1) Preparation of ginger-like saponin R1 solution: Accurately weigh 43 mg of ginger-like saponin R1, add it to 3.6 mL of deionized water, and sonicate for 30 minutes to obtain a ginger-like saponin R1 solution with a concentration of 15 mmol / L; (2) Preparation of strontium ion solution: Weigh 85 mg of strontium nitrate, add it to 10 mL of deionized water, stir for 10 minutes to obtain a strontium ion solution with a concentration of 40 mmol / L; (3) Preparation of self-assembled hydrogel: Mix the two solutions of the above with equal volumes, stir at 150 r / min for 3 minutes, and let stand at room temperature for 5 minutes to obtain a white ginger-like notoginseng saponin R1-strontium ion hydrogel (e.g. Figure 4 (As shown).

[0043] Example 4 The preparation method of the self-assembled hydrogel of ginger-like notoginsenoside R1 and iron ions includes the following steps: (1) Preparation of ginger-like saponin R1 solution: Accurately weigh 43 mg of ginger-like saponin R1, add it to 3.6 mL of deionized water, and sonicate for 30 minutes to obtain a ginger-like saponin R1 solution with a concentration of 15 mmol / L; (2) Preparation of ferric ion solution: Weigh 20 mg of ferric chloride, dissolve it in a small amount of distilled water, transfer it to a volumetric flask (or graduated test tube), make up to 8.2 mL, and shake well to obtain a 15 mmol / L ferric ion solution; (3) Preparation of self-assembled hydrogel: Mix the two solutions of the above with equal volumes, stir at 150 r / min for 3 minutes, and let stand at room temperature for 5 minutes to obtain brown ginger-like Panax notoginseng saponin R1-iron ion hydrogel (e.g. Figure 5 (As shown).

[0044] Example 5 The preparation method of the self-assembled hydrogel of ginger-like notoginsenoside R1 and indium ions includes the following steps: (1) Preparation of ginger-like saponin R1 solution: Accurately weigh 43 mg of ginger-like saponin R1, add it to 3.6 mL of deionized water, and sonicate for 30 minutes to obtain a ginger-like saponin R1 solution with a concentration of 15 mmol / L; (2) Preparation of indium ion solution: Weigh 10 mg of indium chloride, dissolve it in a small amount of distilled water, transfer it to a graduated container, make up to 3.0 mL, and shake well to obtain a 15 mmol / L solution; (3) Preparation of self-assembled hydrogel: Mix the two solutions of the above with equal volumes, stir at 150 r / min for 3 minutes, and let stand at room temperature for 5 minutes to obtain a white ginger-like notoginseng saponin R1-indium ion hydrogel (e.g. Figure 6 (As shown).

[0045] Example 6 The preparation method of the self-assembled hydrogel of ginger-like notoginsenoside R1 and gallium ions includes the following steps: (1) Preparation of ginger-like saponin R1 solution: Accurately weigh 43 mg of ginger-like saponin R1, add it to 3.6 mL of deionized water, and sonicate for 30 minutes to obtain a ginger-like saponin R1 solution with a concentration of 15 mmol / L; (2) Preparation of gallium ion solution: Weigh 10 mg gallium chloride, dissolve it in a small amount of distilled water, transfer it to a graduated container, make up to 3.8 mL, and shake well to obtain a 15 mmol / L solution; (3) Preparation of self-assembled hydrogel: Mix the two solutions of the above with equal volumes, stir at 150 r / min for 3 minutes, and let stand at room temperature for 5 minutes to obtain a white ginger-like notoginseng saponin R1-gallium ion hydrogel (e.g. Figure 7 (As shown).

[0046] Example 7 Compared with Example 4, the only difference is that the concentration of the ferric chloride solution is adjusted to 5 mmol / L, while the other steps and conditions are the same as in Example 4.

[0047] Example 8 Compared with Example 4, the only difference is that the concentration of the ferric chloride solution is adjusted to 10 mmol / L, while the other steps and conditions are the same as in Example 4.

[0048] The hydrogels obtained in Examples 4 and 7-8 were inverted for 30 min and 48 h, respectively, and the results are as follows: Figure 8 As shown, the color of the hydrogel deepens with increasing iron ion solution concentration, and it still maintains a good gel state after being inverted for 48 hours, indicating that the hydrogel prepared by this invention has excellent stability.

[0049] Comparative Example 1 Compared with Example 4, the only difference is that ferric chloride is removed, while the other steps and conditions are the same as in Example 4.

[0050] The results are as follows Figure 9 As shown, Comparative Example 1 could not form a hydrogel after removing ferric chloride, and could not hang on the upper part of the test tube when inverted. This indicates that ginger-like saponin R1 cannot form a hydrogel when mixed with water in equal volumes.

[0051] In summary, the self-assembled hydrogel of this invention comprises zingibroside R1 and metal ions (divalent and trivalent metal ions); wherein the metal ions are distributed within zingibroside R1. This self-assembled hydrogel exhibits excellent stability. Furthermore, the preparation process of this invention is simple and can produce large quantities of zingibroside R1-metal ion hydrogels at room temperature, and its applications can be expanded by adding drug carriers. Therefore, it shows broad application prospects in the treatment of microbial infections and tumor diseases in complex biological environments.

[0052] The specific embodiments of the present invention described above do not constitute a limitation on the scope of protection of the present invention. Any other corresponding changes and modifications made in accordance with the technical concept of the present invention should be included within the scope of protection of the claims of the present invention.

Claims

1. A ginsenoside Rl-metal ion self-assembled hydrogel, comprising ginsenoside Rl, metal ions and a solvent; wherein, The concentration of the notoginseng saponin R1 and the metal ion is 1-100 mmol / L; the metal ion includes at least one of a divalent metal ion and a trivalent metal ion.

2. The ginger-like notoginseng saponin Rl-metal ion self-assembled hydrogel according to claim 1, characterized in that, The concentration of the notoginseng saponin R1 is 5-50 mmol / L.

3. The ginger-like notoginseng saponin Rl-metal ion self-assembled hydrogel according to claim 1, characterized in that, The concentration of the divalent metal ion is 10-40 mmol / L; The concentration of the trivalent metal ion is 5-20 mmol / L.

4. The ginger-like notoginseng saponin R1-metal ion self-assembled hydrogel of claim 1, characterized in that, The divalent metal ion includes at least one of a copper ion, a zinc ion, a calcium ion, a strontium ion and a magnesium ion; The trivalent metal ion includes at least one of a gold ion, an iron ion, an indium ion and a gallium ion.

5. The ginger-like notoginseng saponin Rl-metal ion self-assembled hydrogel of claim 1, characterized in that, The solvent includes at least one of water, an ethanol solution and a buffer solution with a pH value of 6-8.

6. The preparation method of the ginger-like notoginseng saponin Rl-metal ion self-assembly hydrogel according to any one of claims 1-5, characterized in that, The method comprises the following steps: Mixing the notoginseng saponin R1 solution and the soluble metal source solution uniformly, and standing to form a self-assembled hydrogel at 10-30℃.

7. The preparation method of the ginger-like notoginseng saponin Rl-metal ion self-assembled hydrogel according to claim 6, characterized in that, The notoginseng saponin R1 solution is prepared by adding the notoginseng saponin R1 into a first solvent and stirring uniformly; The soluble metal source solution is prepared by dissolving a soluble metal source in a second solvent; The first solvent and the second solvent are each independently selected from at least one of water, an ethanol solution and a buffer solution with a pH value of 6-8.

8. The method for preparing the ginger-like notoginseng saponin Rl-metal ion self-assembly hydrogel according to claim 6, characterized in that, The soluble metal source includes at least one of a soluble copper salt, a soluble zinc salt, a soluble calcium salt, a soluble strontium salt, a soluble magnesium salt, a soluble gold acid and gold salt, a soluble iron salt, a soluble indium salt and a soluble gallium salt.

9. The method for preparing the ginger-like notoginseng saponin Rl-metal ion self-assembly hydrogel according to claim 8, characterized in that, The soluble copper salt is selected from one or more of copper sulfate, copper chloride and copper acetate; The soluble zinc salt is selected from one or more of zinc nitrate, zinc chloride and zinc acetate; The soluble calcium salt is selected from one or more of calcium chloride, calcium nitrate and calcium acetate; The soluble strontium salt is selected from one or more of strontium nitrate, strontium chloride and strontium acetate; The soluble magnesium salt is selected from one or more of magnesium chloride, magnesium nitrate and magnesium phosphate; The soluble gold acid and gold salt are selected from one or more of chloroauric acid, gold chloride, sodium chloroaurate, potassium chloroaurate and gold nitrate; The soluble iron salt is selected from one or more of iron chloride, iron nitrate and iron sulfate; The soluble indium salt is selected from one or more of indium chloride, indium nitrate and indium sulfate; The soluble gallium salt is selected from one or more of gallium chloride, gallium nitrate and gallium sulfate.

10. The method of claim 6, wherein the preparation of the self-assembled hydrogel of ginsenoside Rl-metal ion is characterized by, The standing temperature is 20-25℃; The standing time is 1-1440 min.

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