Schistosoma japonicum katsurada recombinant antigens rSjCB1 and rSjSAP4 and application thereof

By preparing and applying recombinant antigens rSjCB1 and rSjSAP4 from Schistosoma japonicum, the problems of insufficient sensitivity and specificity in the diagnosis of sheep schistosomiasis were solved, and efficient and accurate detection of sheep schistosomiasis was achieved, especially through ELISA and detection chip methods.

CN121673385APending Publication Date: 2026-03-17SHANGHAI VETERINARY RESEARCH INSTITUTE CAAS (CHINESE ANIMAL HEALTH & EPIDEMIOLOGY CENTER SHANGHAI BRANCH)
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Patent Information

Application Number
CN202511497183.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-10-20
Publication Date
2026-03-17

AI Technical Summary

Technical Problem

Currently, there is a lack of sensitive and specific antigens for the diagnosis of sheep schistosomiasis. Traditional fecal egg detection is time-consuming and laborious. Existing serological diagnostic methods, such as SEA, are cumbersome to prepare and have complex components, making it difficult to meet the needs of high-throughput and sensitive and specific monitoring.

Method used

Two recombinant antigens of Schistosoma japonicum, rSjCB1 and rSjSAP4, are provided and prepared by expression using recombinant vectors. They are used for detection by ELISA and detection chips, and can be detected in combination with ELISA plates, positive control serum, negative control serum, enzyme-labeled secondary antibody, and chromogenic solution.

Benefits of technology

It achieves high sensitivity (97.87%) and high specificity (81%) detection of sheep schistosomiasis, and has no cross-reactivity with other schistosomiasis diseases. The combined detection sensitivity is 93.62% and the specificity is 92%, meeting the monitoring requirements of high throughput and sensitive and specificity.

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Abstract

The invention belongs to the technical field of bioengineering, and relates to schistosoma japonicum recombinant antigens rSjCB1 and rSjSAP4 and application thereof. The schistosoma japonicum recombinant antigen rSjCB1 is used as a detection antigen for schistosomiasis diagnosis, has sensitivity of 97.87% and specificity of 81% on sheep schistosoma japonicum, and has no cross reaction with sheep serum infected by pichia pastoris and haemonchus contortus. The combined detection result of the rSjCB1 and the rSjSAP4 shows that the sensitivity is 93.62%, and the specificity is 92%, so that the recombinant antigen rSjCB1, the rSjCB1 and the rSjSAP4 prepared by the invention are used for diagnosing the sheep schistosomiasis, are high in sensitivity and strong in specificity, and have important significance in preventing and treating the sheep schistosomiasis.
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