High-value utilization method of flavonoid-rich forest medicine resources

Abstract

The invention relates to the technical field of forest medicine resource development. The invention provides a high-valued utilization method of a flavonoid-rich forest medicine resource, which comprises the following steps: (1) preliminarily drying a forest medicine raw material, and then crushing and sieving to obtain raw material powder; (2) mixing the raw material powder with an ethanol solution, and performing ultrasonic treatment to obtain a mixed solution; (3) adding glycerol and sodium bicarbonate into the mixed solution, carrying out secondary ultrasonic treatment, centrifuging, and separating supernate and residues; and (4) drying the supernate to obtain the extract containing the flavonoid compounds. A traditional organic solvent extraction mode is improved, a deep-eutectic solvent extraction method is introduced, the extraction efficiency is effectively improved by integrating the traditional organic solvent extraction mode and the deep-eutectic solvent extraction method, and the extraction mode has low requirements on instruments, can well adapt to existing factory instruments and is convenient to popularize.

Description

Technical Field

[0001] This invention relates to the field of forest medicinal herb development technology, and in particular to a method for high-value utilization of forest medicinal herb resources rich in flavonoids. Background Technology

[0002] Forest medicinal resources refer to medicinal resources cultivated based on forest ecosystems, encompassing two major categories: plant-based and animal-based medicines. my country currently has 15,321 species of medicinal plants, 86.3% of which originate from forests. Forest-derived medicinal materials are an important source of high-quality medicinal materials in my country, with varieties such as Polygonatum sibiricum, Panax notoginseng, and Dendrobium officinale already achieving large-scale cultivation.

[0003] Flavonoids are widely found in forest medicinal resources and possess various biological activities such as antioxidant, anti-inflammatory, and antibacterial properties, making them widely used in the pharmaceutical, health product, and cosmetic fields. However, traditional extraction methods suffer from low efficiency, high solvent residue, and high energy consumption. Therefore, developing an efficient, environmentally friendly, and industrially viable method for extracting flavonoids is of paramount importance. Summary of the Invention

[0004] The purpose of this invention is to provide a method for the high-value utilization of forest medicinal resources rich in flavonoids, which significantly improves the extraction efficiency of flavonoids from forest medicinal resources.

[0005] To achieve the above-mentioned objectives, the present invention provides the following technical solution: This invention provides a method for the high-value utilization of flavonoid-rich forest medicinal resources, comprising the following steps: (1) The raw materials of the forest medicinal materials are initially dried, then crushed and sieved to obtain raw material powder; (2) Mix the raw material powder with an ethanol solution and sonicate to obtain a mixture; (3) Add glycerol and sodium bicarbonate to the mixture, sonicate twice, and centrifuge to separate the supernatant and residue; (4) The supernatant was dried to obtain an extract containing flavonoids.

[0006] Preferably, the types of medicinal materials mentioned in step (1) are hawthorn, ginkgo, honeysuckle, licorice, scutellaria, and dandelion.

[0007] Preferably, the temperature of the initial drying in step (1) is 40~50℃, the moisture content after drying is ≤12%, and the mesh size of the sieve during sieving is 80~100 mesh.

[0008] Preferably, the concentration of the ethanol solution in step (2) is 70-80%, and the ratio of the raw material powder to the ethanol solution is 1g:6-8mL.

[0009] Preferably, the ultrasonic power in step (2) is 125~135W, the ultrasonic temperature is 40~45℃, and the ultrasonic time is 8~10min.

[0010] Preferably, the amount of glycerol added in step (3) is 35-45% of the total volume of the mixture, and the amount of sodium bicarbonate added in the mixture is 40-50 g / L.

[0011] Preferably, the power of the secondary ultrasound in step (3) is 140~150W, the temperature of the ultrasound is 40~45℃, and the duration of the ultrasound is 10~14min.

[0012] Preferably, the centrifugation speed in step (3) is 2500~2700 r / min, and the centrifugation time is 10~12 min.

[0013] Preferably, freeze drying is used during the drying process described in step (4).

[0014] This invention provides a method for high-value utilization of forest medicinal resources rich in flavonoids, comprising the following steps: (1) pre-drying the forest medicinal raw materials, then pulverizing and sieving to obtain raw material powder; (2) mixing the raw material powder with an ethanol solution and sonicating to obtain a mixture; (3) adding glycerol and sodium bicarbonate to the mixture, sonicating twice and centrifuging to separate the supernatant and residue; (4) drying the supernatant to obtain an extract containing flavonoids.

[0015] This invention improves upon traditional organic solvent extraction methods by introducing a combination of glycerol and ethanol. These two compounds, together with sodium bicarbonate, form a hydrogen bond donor-acceptor pair, increasing the polarity of the solution and facilitating the extraction of active ingredients. Furthermore, sodium bicarbonate generates microbubbles during ultrasonication, further enhancing extraction efficiency. This solvent modification not only increases the extraction rate but also effectively reduces the ultrasonic power and time required during extraction, shortening the extraction time and improving the activity of the extract.

[0016] The extracted substance is a mixture containing various flavonoids, which can be flexibly separated and utilized according to subsequent needs, providing a strong foundation for the development of flavonoid products. Detailed Implementation

[0017] This invention provides a method for the high-value utilization of flavonoid-rich forest medicinal resources, comprising the following steps: (1) The raw materials of the forest medicinal materials are initially dried, then crushed and sieved to obtain raw material powder; (2) Mix the raw material powder with an ethanol solution and sonicate to obtain a mixture; (3) Add glycerol and sodium bicarbonate to the mixture, sonicate twice, and centrifuge to separate the supernatant and residue; (4) The supernatant was dried to obtain an extract containing flavonoids.

[0018] In this invention, the types of medicinal materials mentioned in step (1) are hawthorn, ginkgo, honeysuckle, licorice, scutellaria, and dandelion.

[0019] In this invention, the temperature of the preliminary drying in step (1) is preferably 40~50℃, more preferably 45℃, the moisture content after drying is preferably ≤12%, more preferably ≤10%, and the mesh size of the sieve during sieving is preferably 80~100 mesh, more preferably 90 mesh.

[0020] In this invention, the concentration of the ethanol solution in step (2) is preferably 70-80%, more preferably 75%, and the ratio of the raw material powder to the ethanol solution is preferably 1g:6-8mL, more preferably 1g:7mL.

[0021] In this invention, the power of the ultrasound in step (2) is preferably 125~135W, more preferably 130W, the temperature of the ultrasound is preferably 40~45℃, more preferably 45℃, and the duration of the ultrasound is preferably 8~10min, more preferably 9min.

[0022] In this invention, the amount of glycerol added in step (3) is preferably 35-45% of the total volume of the mixture, more preferably 40%, and the amount of sodium bicarbonate added in the mixture is preferably 40-50 g / L, more preferably 45 g / L.

[0023] In this invention, the power of the secondary ultrasound in step (3) is preferably 140~150W, more preferably 145W, the temperature of the ultrasound is preferably 40~45℃, more preferably 45℃, and the time of the ultrasound is preferably 10~14min, more preferably 12min.

[0024] In this invention, the centrifugation speed in step (3) is preferably 2500~2700 r / min, more preferably 2600 r / min, and the centrifugation time is preferably 10~12 min, more preferably 11 min.

[0025] In this invention, freeze drying is preferably used during the drying process described in step (4).

[0026] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0027] Example 1

[0028] A method for high-value utilization of flavonoid-rich forest medicinal resources includes the following steps: (1) Ginkgo leaves were initially dried at 40°C until the moisture content was ≤12%, then pulverized and passed through a 100-mesh sieve to obtain raw material powder; (2) Mix the raw material powder with 70% ethanol solution at a ratio of 1g:8mL, and sonicate at 125W and 45℃ for 8min to obtain a mixture; (3) Add 45% glycerol of the total volume of the mixture to the mixture, then add sodium bicarbonate according to the standard of 40 g / L, sonicate twice for 10 min at 150 W and 45 °C, then centrifuge for 10 min at 2700 r / min to separate the supernatant and residue. (4) Freeze-dry the supernatant to obtain an extract containing flavonoids.

[0029] Results: In this example, 100g of dried Ginkgo biloba leaves were used for the experiment, and a total of 1.82g of extract containing flavonoids was obtained. The total flavonoid content in the extract was 47.12%.

[0030] Example 2

[0031] A method for high-value utilization of flavonoid-rich forest medicinal resources includes the following steps: (1) Scutellaria baicalensis was initially dried at 50°C until the moisture content was ≤12%, then pulverized and passed through an 80-mesh sieve to obtain raw material powder; (2) Mix the raw material powder with 80% ethanol solution at a ratio of 1g:6mL, and sonicate at 135W and 40℃ for 10min to obtain a mixture; (3) Add 35% glycerol of the total volume of the mixture to the mixture, then add sodium bicarbonate according to the standard of 50 g / L, sonicate twice for 14 min at 140 W and 40 °C, then centrifuge for 12 min at 2500 r / min to separate the supernatant and residue. (4) Freeze-dry the supernatant to obtain an extract containing flavonoids.

[0032] Results: In this example, 100g of dried Scutellaria baicalensis was used for the experiment, and a total of 2.09g of extract containing flavonoids was obtained. The total flavonoid content in the extract was 43.02%.

[0033] Example 3

[0034] A method for high-value utilization of flavonoid-rich forest medicinal resources includes the following steps: (1) The licorice was initially dried at 45℃ until the moisture content was ≤12%, then pulverized and passed through a 90-mesh sieve to obtain raw material powder; (2) Mix the raw material powder with 75% ethanol solution at a ratio of 1g:7mL, and sonicate at 130W and 42℃ for 9min to obtain a mixture; (3) Add 40% glycerol of the total volume of the mixture to the mixture, then add sodium bicarbonate according to the standard of 45 g / L, sonicate twice for 12 min at 145 W and 42 °C, then centrifuge for 11 min at 2600 r / min to separate the supernatant and residue. (4) Freeze-dry the supernatant to obtain an extract containing flavonoids.

[0035] Results: In this example, 100g of dried licorice was used for the experiment, and a total of 2.61g of extract containing flavonoids was obtained. The total flavonoid content in the extract was found to be 50.84%.

[0036] Comparative Example 1

[0037] Glycerin and sodium bicarbonate were not used in the extraction process. The remaining steps and parameters were the same as in Example 1. The results showed that 0.86g of extract containing flavonoids was obtained by using 100g of dried ginkgo leaves in the same experiment. The total flavonoid content in the extract was 39.51%.

[0038] Comparative Example 2

[0039] Glycerin and sodium bicarbonate were not used in the extraction process. The remaining steps and parameters were the same as in Example 2. The results showed that 100g of dried Scutellaria baicalensis was used in the experiment, and a total of 1.21g of extract containing flavonoids was obtained. The total flavonoid content in the extract was 37.11%.

[0040] Comparative Example 3

[0041] Glycerin and sodium bicarbonate were not used in the extraction process. The remaining steps and parameters were the same as in Example 3. The results showed that using 100g of dried licorice, a total of 1.37g of extract containing flavonoids was obtained, and the total flavonoid content in the extract was determined to be 35.10%.

[0042] As can be seen from the above embodiments, the present invention provides a method for high-value utilization of flavonoid-rich forest medicinal resources, including the following steps: (1) pre-drying the forest medicinal raw materials, then pulverizing and sieving to obtain raw material powder; (2) mixing the raw material powder with an ethanol solution, and ultrasonicating to obtain a mixture; (3) adding glycerol and sodium bicarbonate to the mixture, ultrasonicating twice, and centrifuging to separate the supernatant and residue; (4) drying the supernatant to obtain an extract containing flavonoid compounds. The present invention improves the traditional organic solvent extraction method by introducing a eutectic solvent extraction method. By integrating the two, the extraction efficiency is effectively improved, and the extraction method does not require high-end instruments, can be well adapted to existing factory instruments, and is easy to promote.

[0043] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A method for high-value utilization of flavonoid-rich medicinal resources, characterized in that, Includes the following steps: (1) The raw materials of the forest medicinal materials are initially dried, then crushed and sieved to obtain raw material powder; (2) Mix the raw material powder with an ethanol solution and sonicate to obtain a mixture; (3) Add glycerol and sodium bicarbonate to the mixture, sonicate twice, and centrifuge to separate the supernatant and residue; (4) The supernatant was dried to obtain an extract containing flavonoids.

2. The utilization method according to claim 1, characterized by, The types of medicinal materials mentioned in step (1) are hawthorn, ginkgo, honeysuckle, licorice, scutellaria, and dandelion.

3. The utilization method according to claim 2, characterized by, The temperature of the initial drying in step (1) is 40~50℃, the moisture content after drying is ≤12%, and the mesh size of the sieve during sieving is 80~100 mesh.

4. The utilization method according to claim 3, characterized by, The concentration of the ethanol solution in step (2) is 70-80%, and the ratio of the raw material powder to the ethanol solution is 1g:6-8mL.

5. The utilization method according to claim 4, characterized in that, The ultrasonic power in step (2) is 125~135W, the ultrasonic temperature is 40~45℃, and the ultrasonic time is 8~10min.

6. The utilization method according to claim 5, characterized in that, In step (3), the amount of glycerol added is 35-45% of the total volume of the mixture, and the amount of sodium bicarbonate added in the mixture is 40-50 g / L.

7. The utilization method according to claim 6, characterized in that, The power of the secondary ultrasound in step (3) is 140~150W, the temperature of the ultrasound is 40~45℃, and the duration of the ultrasound is 10~14min.

8. The utilization method according to claim 7, characterized in that, The centrifugation speed in step (3) is 2500~2700 r / min, and the centrifugation time is 10~12 min.

9. The method of utilization according to any one of claims 1 to 8, characterized in that, The drying process described in step (4) is freeze drying.