Anti-alopecia composition containing curcuma zedoary extract as well as preparation method and application of anti-alopecia composition

Abstract

The invention belongs to the technical field of daily cosmetics, and particularly relates to an anti-hair loss composition containing a curcuma zedoary extract as well as a preparation method and application of the anti-hair loss composition. The anti-hair loss composition comprises a curcuma zedoary extract, a minoxidil analogue and a tea extract, the mass ratio of the curcuma zedoary extract to the minoxidil analogue to the tea extract is 1: (0.2-5): (0.01-1). According to the anti-hair loss composition containing the curcuma zedoary extract, only three main raw materials are compounded and supplement each other to achieve an obvious synergistic effect, the obvious anti-hair loss and hair growth effects are finally achieved, meanwhile, the anti-hair loss composition has the advantages of being mild, suitable for being used for a long time and capable of rapidly taking effect, the scalp environment can be improved, and the hair loss is reduced. Therefore, the hair conditioner has important application potential in the aspects of hair loss prevention and hair growth.

Description

Technical Field

[0001] This invention belongs to the field of daily cosmetics technology, specifically relating to an anti-hair loss composition containing Curcuma zedoaria extract, its preparation method, and its application. Background Technology

[0002] Hair loss refers to the phenomenon of hair falling out. Normally shed hairs are in the telogen (resting) and catagen (transitional) phases. Because the number of hairs entering the telogen phase and those newly entering the anagen (growth) phase is constantly in dynamic equilibrium, a normal amount of hair is maintained. However, when hair falls out abnormally or excessively, it is called pathological hair loss. There are many causes of hair loss, including androgenetic alopecia, neurogenic alopecia, chemical alopecia, age-related alopecia, and pathological alopecia. Among these, research shows that most hair loss is primarily androgenetic alopecia. Androgenetic alopecia, also known as androgenetic hair loss or seborrheic alopecia, is a type of hair loss related to androgens (mainly testosterone and dihydrotestosterone). Due to the influence of testosterone and dihydrotestosterone, hair follicles accelerate their transition from the anagen to the telogen phase, leading to increased hair loss. This is accompanied by follicle atrophy, causing previously healthy terminal hairs to transform into vellus hairs, ultimately resulting in sparse hair in the balding area, with newly grown hairs being mainly vellus hairs that do not grow long. Currently available medications for androgenetic alopecia include minoxidil, finasteride, and spironolactone, primarily administered orally or topically. While these medications can slow hair loss to some extent, long-term use can lead to side effects such as decreased libido, erectile dysfunction, and menstrual irregularities. Therefore, researching a novel combination drug for androgenetic alopecia is of significant importance in addressing the potential side effects of long-term use of existing medications.

[0003] Current hair loss prevention products on the market often use a combination of multiple ingredients targeting different points, hoping to achieve better results through multi-pathway and multi-target action. However, this combination approach also has some drawbacks: compound products often target general hair loss problems and are difficult to adapt to the specific needs of different individuals, different causes of hair loss, and different degrees of hair loss. Secondly, compound products usually contain multiple ingredients, each with its specific mechanism of action and potential side effects. Furthermore, with the simplification of formulations, the complex combination of ingredients increases the possibility of introducing allergens. Therefore, simplifying the formulation of hair loss prevention products has become an important trend in the development of such products.

[0004] Curcuma zedoaria, also known as black-hearted ginger, blue ginger, or black ginger, belongs to the genus Curcuma in the ginger family. In China, it mainly refers to the rhizomes of three species: Curcuma zedoaria non Rosc., Curcuma kwangsiensis SGLee et CFLiang, and Curcuma wenyujin YHChen et C.Ling. It contains rich volatile oil components and has anti-inflammatory, antibacterial, and blood-activating effects. Chinese patent document CN112043655A discloses a plant-based anti-dandruff composition and a silicone-free shampoo, as well as its preparation method. This composition primarily utilizes an arbitrary combination of extracts from *Curcuma zedoaria*, *Curcuma longa*, and *Nymphaea granatum* to regulate scalp sebum secretion and kill and inhibit the growth of *Malassezia*. Chinese patent document CN111110619A discloses an oil-controlling and acne-removing composition and its application in cosmetics. This composition contains *Curcuma zedoaria*, black willow bark, *Amorphophallus konjac* stem / root, hawthorn, and *Kaempferia galanga* root. Through the synergistic effect of multiple plants, it effectively controls oil and removes acne, and is gentle and non-irritating, suitable for long-term use. Therefore, existing research on *Curcuma zedoaria* only demonstrates its effectiveness in anti-dandruff, antibacterial, oil-controlling, and acne-removing effects, without exploring its application in the field of hair loss prevention and hair growth.

[0005] Currently, the clinical efficacy of Curcuma zedoaria is mainly reflected in its effectiveness in treating menstrual disorders and masses, abdominal pain, and hypochondriac distension caused by qi stagnation and blood stasis. These symptoms are somewhat related to hair loss, therefore Curcuma zedoaria may have potential value in treating hair loss symptoms. Therefore, researching and developing a new, simplified formula of anti-hair loss drug with Curcuma zedoaria as the main ingredient to treat androgenetic alopecia is of great significance. Summary of the Invention

[0006] The present invention aims to provide a hair loss prevention composition containing Curcuma zedoaria extract and its application. It is made by compounding only three main raw materials, which complement each other and have a significant synergistic effect, ultimately achieving significant hair loss prevention and hair growth effects. At the same time, it can also improve the scalp environment and maintain the hair in a healthy state.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] The first objective of this invention is to provide a hair loss prevention composition containing Curcuma zedoaria extract, the hair loss prevention composition comprising Curcuma zedoaria extract, minoxidil analog, and tea extract; wherein the mass ratio of Curcuma zedoaria extract, minoxidil analog, and tea extract is 1:(0.2-5):(0.01-1).

[0009] Preferably, the method for preparing the Curcuma zedoaria extract includes:

[0010] The dried turmeric was pulverized and passed through a 20-80 mesh sieve. It was then extracted with ethanol of 50-95% (v / v) at a material-to-liquid ratio of 1g:5-20mL for 0.5-2h, 1-3 times. After filtration, the filtrates were combined and the ethanol was removed by vacuum distillation. The extract was then eluted and enriched by silica gel chromatography. The residue was collected and the eluent was removed by vacuum distillation to obtain the turmeric extract.

[0011] Preferably, the silica gel chromatography column has a pore size of 200-300 mesh; the eluent is 80% ethanol; the preparation method of the Curcuma zedoaria extract further includes: collecting the eluent and concentrating it until no ethanol remains.

[0012] Preferably, the minoxidil analogues include one or both of diaminopyrimidine oxides and pyrrolidinyl diaminopyrimidine oxides.

[0013] Preferably, the tea extract includes at least one of tea leaf extract, tea seed extract, and camellia seed extract.

[0014] Preferably, the anti-hair loss composition further includes a solubilizer, wherein the mass ratio of the solubilizer to the turmeric extract is (1-10):1, and the solubilizer includes one or more of PEG-40 hydrogenated castor oil, Tween-28, Tween-60, Tween-80, PEG-55 propylene glycol oleate, and polyols.

[0015] Preferably, the anti-hair loss composition further includes a solvent, the solvent being water, and the weight of the solvent added is 20-35% of the total mass of the turmeric extract, minoxidil analog, tea extract, and solubilizer.

[0016] A second object of the present invention is to provide a method for preparing the above-mentioned anti-hair loss composition containing Curcuma zedoaria extract, comprising the following steps:

[0017] S1. Weigh out the prescribed amounts of Curcuma zedoaria extract, minoxidil analog, tea extract, and solubilizer, and set aside.

[0018] S2. Place the prescribed amount of Curcuma zedoaria extract in a solubilizer, heat to 40-60℃, homogenize, and obtain mixture A;

[0019] S3. Add the prescribed amount of minoxidil analog to mixture A obtained in step S2, keep it at 40-60℃, homogenize it, and obtain mixture B.

[0020] S4. After the mixture B from step S3 has cooled to below 40°C, add the prescribed amount of tea extract and solvent, homogenize, and the anti-hair loss composition is obtained.

[0021] Preferably, the homogenization speed in steps S2, S3 and S4 is 1500-4000 rpm and the homogenization time is 5-10 min.

[0022] Another object of the present invention is to provide the use of the above-described anti-hair loss composition containing Curcuma zedoaria extract in the preparation of hair care products.

[0023] The design principles and inventive concept of this invention:

[0024] Firstly, this invention uses Curcuma zedoaria as the main ingredient for preventing hair loss. The "Curcuma zedoaria" in the main component of the anti-hair loss composition of this invention refers to the rhizome and tuberous parts of plants in the genus Curcuma, mainly including one or more of Curcuma zedoaria non Rosc., Curcuma kwangsiensis SGLee et CFLiang, and Curcuma wenyujin YHChen et C.Ling. The main active ingredients in Curcuma zedoaria, gemcitabine and furandiene, have strong 5α-reductase inhibitory activity, effectively preventing testosterone from converting into dihydrotestosterone, which has a higher affinity for androgen receptors on the dermal papilla, thus preventing hair follicles from transitioning to the resting phase and causing follicle atrophy, thereby achieving an anti-hair loss effect. Simultaneously, Curcuma zedoaria extract contains abundant 1,8-cineole, which can promote the penetration of minoxidil analogs.

[0025] Secondly, minoxidil is a treatment for androgenetic alopecia. It has a pyrimidine epoxide structure with one (1-piperidine cyclogroup) and two amino groups, giving it the ability to inhibit androgenetic alopecia. Its main mechanism of action is as a K+ channel opener, upregulating VEGF and promoting vasodilation, thus promoting ATP synthesis to provide energy to hair follicles and accelerating the transition of hair follicles from the resting phase to the anagen phase. However, long-term use of minoxidil may cause side effects such as local skin irritation or erythema. Diaminopyrimidine oxides, pyrrolidine diaminopyrimidine oxides, and certain natural extracts such as Platycladus orientalis leaves and Ligustrum lucidum, which have similar structures to minoxidil, are relatively milder and more acceptable for use in cosmetics or health products. This invention uses minoxidil analogs as raw materials for the anti-hair loss composition, specifically including diaminopyrimidine oxides and pyrrolidine diaminopyrimidine oxides. Among them, pyrrolidine diaminopyrimidine oxide and diaminopyrimidine oxide both contain a diaminopyrimidine oxide cyclic structure similar to that of minoxidil. Therefore, their main mechanism of action is similar to that of minoxidil. In addition, diaminopyrimidine oxide also has the effect of inhibiting the network densification of fibronectin and collagen and activating hair follicle opening by inhibiting the synthesis of lysine hydroxylase, and acts on different targets than Curcuma zedoaria extract.

[0026] Furthermore, the tea polyphenols in the tea extract, another key component of the anti-hair loss composition of the present invention, have excellent antioxidant and free radical scavenging effects. On the one hand, they can prevent oxidative stress in hair papilla cells from leading to apoptosis or aging. On the other hand, tea polyphenols can protect the unsaturated bonds on the main active ingredient in Curcuma zedoaria from peroxidation. In addition, tea saponins in the tea extract can also act as surfactants to self-solubilize the oil-soluble components in Curcuma zedoaria extract, increasing its effectiveness in the formulation.

[0027] In summary, the three raw materials of the anti-hair loss composition of the present invention—turmeric extract, minoxidil analogue, and tea extract—can interact to achieve a synergistic effect. Furthermore, the anti-hair loss composition provided by the present invention differs from products on the market in that it can cover more targets while maintaining a simple composition. Moreover, the present invention uses pure plant extracts as the main raw materials of the anti-hair loss composition, and the formula is mild and suitable for long-term use, thus balancing gentleness and high efficacy.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] (1) This invention expands the application of Curcuma zedoaria extract, minoxidil analogue and tea extract as anti-hair loss products. It proposes for the first time a compound combination of Curcuma zedoaria extract, minoxidil analogue and tea extract. Through a large amount of detailed experimental data, it is found that the anti-hair loss composition provided by this invention has significant anti-hair loss and hair growth effects, and can also improve the scalp environment and maintain the hair in a healthy state.

[0030] (2) The anti-hair loss composition provided by the present invention is made by compounding only three main raw materials. The formula is mild, has no toxic side effects, and is suitable for long-term use.

[0031] (3) The preparation method of the anti-hair loss composition of the present invention is simple, convenient and inexpensive, and suitable for large-scale industrial production. Attached Figure Description

[0032] Figure 1 The images show the effects of using the serum from Group A containing the anti-hair loss composition with Curcuma zedoaria extract from Example 1, where left: before use, right: after use;

[0033] Figure 2 The images show the effects of using the Group B serum containing the anti-hair loss composition with Curcuma zedoaria extract from Example 2, where left: before use, right: after use. Detailed Implementation

[0034] The following detailed embodiments further illustrate the above-described content of the present invention. However, this should not be construed as limiting the scope of the present invention to the following embodiments.

[0035] 1. Source of raw materials:

[0036] The diaminopyrimidine oxide was purchased from Guangzhou Huilington Trading Co., Ltd.

[0037] The tea extract was purchased from Xi'an Best Biotechnology Co., Ltd.

[0038] The ginger root extract was purchased from Guangzhou Luojie Biotechnology Co., Ltd.

[0039] The camellia seed extract was purchased from Guangdong Jingcui Biotechnology Co., Ltd.

[0040] The pyrrolyl diaminopyrimidine oxide was purchased from Guangzhou Huilington Trading Co., Ltd.

[0041] All other raw materials or experimental materials used in this invention can be purchased through legitimate commercial channels.

[0042] 2. The tea extract in the anti-hair loss composition formula containing Curcuma zedoaria extract of this invention is a conventional commercially available product. The tea extract is derived from the leaves, stems, seeds, and flowers of plants belonging to the Theaceae family and the Camellia genus. Its preparation method includes: extracting and purifying characteristic components from the leaves, stems, seeds, and flowers of plants belonging to the Theaceae family and the Camellia genus using biological, chemical, or physical methods. The characteristic components are one or more of catechin GC, epigallocatechin EGC, epigallocatechin gallate ester EGCG, epicatechin EC, epicatechin gallate ester ECG, and tea saponins. Therefore, the tea extract of this invention includes one or more of catechins, flavonoids, anthocyanins, phenolic acids, tea saponins, tea polysaccharides, and glycosylated or acetylated extensions containing characteristic components. More preferably, the tea extract of this invention is extracted from leaves and seeds, i.e., tea leaf extract, tea seed extract, and camellia seed extract.

[0043] 3. The solubilizer in the anti-hair loss composition formulation containing Curcuma zedoaria extract of the present invention includes one or more of PEG-40 hydrogenated castor oil, Tween-28, Tween-60, Tween-80, PEG-55 propylene glycol oleate, and polyols; the polyols include, but are not limited to, one or more of 1,3-butanediol, propylene glycol, glycerol, and 1,2-pentanediol.

[0044] 4. Preparation of the Curcuma zedoaria extract of the present invention:

[0045] The dried turmeric was pulverized and passed through a 60-mesh sieve. It was then extracted with 80% ethanol at a material-to-liquid ratio of 1g:10mL for 1 hour, and the extraction was repeated three times. After filtration, the filtrates were combined and the ethanol was removed by conventional vacuum distillation. The turmeric was then eluted and enriched using a silica gel chromatography column (200-300 mesh silica gel). The residue was collected and the eluent was removed by vacuum distillation to obtain the turmeric extract.

[0046] The vacuum distillation is carried out at a pressure of -20 MPa and a temperature of 55°C. The silica gel chromatography column has a pore size of 200–300 mesh. The eluent is 80% ethanol. The preparation method of the Curcuma zedoaria extract further includes: collecting the eluent and concentrating it until no ethanol remains.

[0047] Example 1: Preparation of the anti-hair loss composition containing Curcuma zedoaria extract of the present invention

[0048] The preparation of a hair loss prevention composition containing Curcuma zedoaria extract includes the following steps:

[0049] S1. Weigh out 80 kg of the following ingredients in a mass ratio of 1:0.5:0.5:0.5:5:5:1:0.5 ...

[0050] S2. Place the Curcuma zedoaria extract and pyrrolidine diaminopyrimidine oxide in a solubilizer, heat to 60°C, homogenize at 2000 rpm for 5 min to obtain mixture A;

[0051] S3. Add the diaminopyrimidine oxide to the mixture A obtained in step S2, keep it at 60°C, homogenize at 2000 rpm for 5 min to obtain mixture B.

[0052] S4. After the mixture B from step S3 has cooled to below 40°C, add tea extract and 20kg of water, homogenize at 2000rpm for 5min to obtain the anti-hair loss composition.

[0053] Example 2: Preparation of the anti-hair loss composition containing Curcuma zedoaria extract of the present invention

[0054] The preparation of a hair loss prevention composition containing Curcuma zedoaria extract includes the following steps:

[0055] S1. Weigh out 80 kg of Curcuma zedoaria extract, diaminopyrimidine oxide and tea seed extract in a mass ratio of 1:0.2:1, and set aside.

[0056] S2. Place the Curcuma zedoaria extract in 8 times its mass fraction of the solubilizing agent 1,3-butanediol, heat to 55°C, homogenize at 2000 rpm for 5 min to obtain mixture A;

[0057] S3. Add the diaminopyrimidine oxide to the mixture A obtained in step S2, keep it at 60°C, homogenize at 2000 rpm for 5 min to obtain mixture B.

[0058] S4. After the mixture B from step S3 has cooled to below 40°C, add tea seed extract and 20 kg of water, homogenize at 1500 rpm for 10 min to obtain the anti-hair loss composition.

[0059] Example 3: Preparation of the anti-hair loss composition containing Curcuma zedoaria extract of the present invention

[0060] The preparation of a hair loss prevention composition containing Curcuma zedoaria extract includes the following steps:

[0061] S1. Weigh out 80 kg of Curcuma zedoaria extract, pyrrolidine diaminopyrimidine oxide, tea extract, 1,3-butanediol and PEG-40 hydrogenated castor oil in a mass ratio of 1:5:0.01:2:5, and set aside for later use; wherein, the 1,3-butanediol and PEG-40 hydrogenated castor oil are solubilizers.

[0062] S2. Place the Curcuma zedoaria extract and pyrrolidine diaminopyrimidine oxide in the solubilizer 1,3-butanediol and PEG-40 hydrogenated castor oil, heat to 40°C, homogenize at 1500 rpm for 10 min to obtain mixture A.

[0063] S3. After the mixture B from step S3 has cooled to below 40°C, add tea extract and 20kg of water, homogenize at 1500rpm for 5min to obtain the anti-hair loss composition.

[0064] Comparative Example 1

[0065] Compared with Example 1, the difference in this comparative example is that no Curcuma zedoaria extract is added during the preparation of the anti-hair loss composition.

[0066] Comparative Example 2

[0067] Compared with Example 1, the difference in this comparative example is that no minoxidil analogue is added during the preparation of the hair loss prevention composition.

[0068] Comparative Example 3

[0069] Compared with Example 1, the difference in this comparative example is that no tea extracts are added during the preparation of the anti-hair loss composition.

[0070] Comparative Example 4

[0071] Compared with Example 1, the difference in this comparative example is that the turmeric extract was replaced with ginger root extract in the preparation process of the anti-hair loss composition.

[0072] Comparative Example 5

[0073] Compared with Example 1, the difference in this comparative example is that the mass ratio of Curcuma zedoaria extract, minoxidil analog and tea extract in the preparation process of the anti-hair loss composition is 1:8:0.5.

[0074] Comparative Example 6

[0075] Compared with Example 1, the difference in this comparative example is that the mass ratio of Curcuma zedoaria extract, minoxidil analog and tea extract in the preparation process of the anti-hair loss composition is 1:1:5.

[0076] Comparative Example 7

[0077] Compared with Example 1, the difference in this comparative example is that the mass ratio of Curcuma zedoaria extract, minoxidil analog and tea extract in the preparation process of the anti-hair loss composition is 1:0.1:0.5.

[0078] Experiment 1: Anti-hair loss serum experiment

[0079] The test was conducted according to Chapter 8, Test Methods for Human Efficacy Evaluation, Section 6, Test Methods for Anti-Hair Loss Efficacy of Cosmetics, of the "Cosmetic Safety Technical Specifications (2015 Edition)".

[0080] Essences (groups A to J) were prepared using the compositions obtained in Examples 1-3 and Comparative Examples 1-7 of the present invention. A base placebo group and an essence prepared using minoxidil were used as control groups. The preparation formulas of the essences are shown in Table 1 below. Then, actual human tests were conducted according to the "Cosmetic Safety Technical Specifications (2015 Edition)" to test the anti-hair loss efficacy of the compositions of the present invention.

[0081] The efficacy evaluation trial included 360 participants aged 18-60 years who experienced significant hair loss and mild thinning, and whose hair loss count exceeded 10 strands in 60 combing attempts. Among them, 215 were male and 175 were female. Participants were randomly divided into 12 groups of 30 each, using minoxidil serum, serums from Examples 1-3, and serums from Comparative Examples 1-7, respectively. A placebo group served as a blank control. The method of use was as follows: 3-5 mL of serum was applied to the affected area twice daily, morning and evening, for 3 consecutive months. Hair loss data from the 60 combing attempts were recorded, the hair loss change rate was calculated, and full-head photographs were taken for comparison. The hair loss change rate (%) was calculated as: (Hair loss after use - Hair loss before use) / Hair loss before use.

[0082] Table 1 Test serum formulations

[0083]

[0084]

[0085] The preparation method of the serum includes: placing deionized water in a container, slowly adding it to 85°C, pre-mixing hyaluronic acid and 1,3-propanediol externally, adding them to the container containing deionized water while homogenizing, homogenizing at a speed of 2000 rpm or higher, homogenizing for 10 minutes until no particles are present, then cooling down, adding the compositions of Examples 1-3 or Comparative Examples 1-7 when the temperature is lowered to below 40°C, stirring evenly to obtain the serum to be tested.

[0086] Table 2. Experimental Results of Hair Loss Change Rate

[0087] test sample Hair loss change rate in 1 month Hair loss change rate in 2 months Hair loss change rate in 3 months Minoxidil 1.1％ -26.6％ -35.9％ Example 1 -13.7％ -27.1％ -35.2％ Example 2 -9.4％ -25.3％ -34.8％ Example 3 -10.2％ -26.4％ -34.3％ Comparative Example 1 -6.3％ -16.5％ -19.2％ Comparative Example 2 -5.4％ -13.7％ -15.9％ Comparative Example 3 -7.7％ -15.2％ -26.8％ Comparative Example 4 -6.9％ -13.3％ -17.9％ Comparative Example 5 -1.3％ -3.5％ -6.8％ Comparative Example 6 -2.6％ -5.9％ -3.7％ Comparative Example 7 -1.7％ -4.2％ -5.3％ placebo 0.9％ -0.5％ 0.7％

[0088] As shown in Table 2 above, the results in Table 2 show that, in the stay-on product, the essence prepared using the anti-hair loss composition containing Curcuma zedoaria extract of the present invention can approach the anti-hair loss effect of minoxidil in 3 months. The effect is even better when diaminopyrimidine oxide and pyrrolidinyl diaminopyrimidine oxide are used in combination. That is, Example 1 is the best embodiment of the present invention. Compared with Examples 1-3, Comparative Examples 1-4 adjusted the main components of the compositions: Curcuma zedoaria extract, minoxidil analog, and tea extract. In Comparative Examples 1-4, the anti-hair loss effect of the serums prepared from the missing or substituted components was significantly reduced. This indicates that the combination of Curcuma zedoaria extract, minoxidil analog, and tea extract has a synergistic effect. In Comparative Example 4, Curcuma zedoaria extract was replaced with ginger root extract. Ginger and Curcuma zedoaria are both plants in the ginger family and are commonly found in anti-hair loss and hair growth product formulations. Gingerol and curcumin in ginger root extract are believed to promote blood circulation in hair follicles. In the test, the anti-hair loss effect of the Comparative Example 4 composition with added ginger root extract was only slightly better than that of the Comparative Example 1 composition. Therefore, the absence or substitution of any one of these components will reduce the actual effect. All groups showed a significant anti-hair loss effect compared to the placebo group. Meanwhile, comparative examples 5-7 further adjusted the content ratio of the anti-hair loss composition. The experimental results showed that the dosage of Curcuma zedoaria extract, minoxidil analog, and tea extract needed to be adjusted according to the ratio set in this invention to achieve better anti-hair loss effect. This further indicates that the formulation of Curcuma zedoaria extract, minoxidil analog, and tea extract directly affects the effect of the anti-hair loss composition of this invention.

[0089] Figure 1 , Figure 2 The images show the effects of using the serums in Group A and Group B, with left: before use and right: after use. It is evident that using the serums prepared using the compositions obtained in Examples 1 and 2 of this invention (i.e., Group A and Group B) resulted in significantly thicker hair, demonstrating that the anti-hair loss composition containing Curcuma zedoaria extract of this invention has a significant effect on preventing hair loss and promoting hair growth.

[0090] Experiment 2: Anti-hair loss shampoo test

[0091] The anti-hair loss compositions prepared using Examples 1-3 of the present invention were formulated into shampoos according to the formulas in Table 3 below and subjected to actual human trials. The base placebo group was used as a control to test the anti-hair loss efficacy of the compositions of the present invention.

[0092] The efficacy evaluation test included 200 participants aged 18-60 years who experienced significant hair loss and mild thinning, with more than 10 hairs lost in a 60-comb test. There were 110 men and 90 women. Participants were randomly divided into four groups of 50 each. One group used the shampoo containing the compositions of Examples 1-3, while the other group used a placebo shampoo. Usage was the same as regular shampoo, and the shampoo was used continuously for one month. Self-evaluations of hair loss after using the shampoos containing the compositions of Examples 1-3 and the placebo shampoo were recorded. The self-evaluation criteria are shown in Table 4 below: Hair loss improvement rate % = (Number of people with improved hair + Number of people with significant improvement) / Total number of respondents.

[0093] Table 3. Tested Shampoo Formulas

[0094]

[0095]

[0096] Shampoo preparation method: Add water and powdered raw materials from phase A to a container, and slowly add the materials while homogenizing at 2000 rpm until completely dissolved. Then start heating. After heating to 60°C, add sodium lauryl ether sulfate from phase A and stir until dissolved. After heating to 85°C, add the remaining raw materials from phase A, keep warm and stir for 30 minutes, then start cooling. After cooling to 45°C, add raw materials from phase B. When cooling to below 40°C, add raw materials from phase C and stir evenly. Then add raw materials from phase D and stir for 30 minutes to obtain the desired shampoo.

[0097] Table 4. Self-assessment of hair loss before and after using the shampoos containing the compositions from Examples 1-3 and the placebo shampoo.

[0098]

[0099] As shown in Table 4, the anti-hair loss composition prepared by the present invention showed a significantly better anti-hair loss improvement rate than the placebo group in the 1-month test results of the wash-off product. In other words, the anti-hair loss composition provided by the present invention can maintain a good anti-hair loss effect when added to the wash-off product.

[0100] Experiment 3 Safety Test

[0101] (1) Test sample: Prepare the corresponding aqueous solution of the compositions of Examples 1 to 3 at a mass fraction of 5%.

[0102] (2) Test population: 90 healthy subjects aged 20 to 50 years with no history of skin allergies were selected and randomly divided into 3 groups of 30 people each.

[0103] (3) Test method: First, clean the skin area to be tested with physiological saline and wait for it to dry for 5 minutes; select a qualified patch tester (purchased from Beijing Baiyiyida Technology Development Co., Ltd., 10 chambers / patch), and use a closed patch test method. Take about 0.02g of the solution sample prepared in Examples 1 to 3 into the patch tester, and cover it with special tape on the inside of the subject's arm. During the test, the test site should not come into contact with water, exercise, scratching, etc. After 48 hours, remove the tester and mark it. After 30 minutes, when the indentation disappears, judge under sufficient light. Record the results with reference to the skin reaction grade standard in the "Cosmetic Safety Technical Specifications 2015 Edition". The skin reaction grading standard is shown in Table 5 below.

[0104] (4) Test results: as shown in Table 6.

[0105] Table 5. Skin Reaction Grading Standards

[0106]

[0107] Table 6 Safety Tests of Anti-Hair Loss Compositions

[0108]

[0109]

[0110] As can be seen from the results in Table 6, the skin patch test results of the anti-hair loss compositions prepared in Examples 1 to 3 of the present invention were all negative at a usage amount of 5% by mass, indicating that the anti-hair loss compositions prepared in Examples 1 to 3 of the present invention have no potential adverse reactions on human skin and have advantages such as high safety and mild effect.

[0111] In summary, the anti-hair loss composition containing Curcuma zedoaria extract provided by this invention is made by combining only three raw materials, which complement each other and have a significant synergistic effect. It has significant anti-hair loss and hair growth effects, and has the advantages of being mild, suitable for long-term use, and fast-acting. It can also improve the scalp environment and maintain the hair in a healthy state, and has important application potential in the prevention of hair loss and hair growth.

[0112] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in the present invention should still be covered by the claims of the present invention.

Claims

1. An anti-hair loss composition comprising a zedoary extract, characterized in that, The anti-hair loss composition comprises zedoary extract, minoxidil analogues, tea extract; the mass ratio of the zedoary extract, the minoxidil analogues and the tea extract is 1:(0.2-5):(0.01-1).

2. The hair loss prevention composition containing the extract of Curcuma zedoary according to claim 1, characterized in that, The preparation method of the zedoary extract comprises the following steps: The dried zedoary is crushed to pass through a 20-80 mesh sieve, and is extracted with ethanol with a volume concentration of 50-95% at a solid-liquid ratio of 1 g:5-20 mL, the extraction time is 0.5-2 h, the extraction is performed for 1-3 times, the filtrates are combined after filtration, and then the ethanol is removed by reduced pressure distillation, and then the eluent is removed by reduced pressure distillation after the elution and enrichment of the zedoary extract through a silica gel chromatographic column, thereby obtaining the zedoary extract.

3. The hair loss prevention composition containing the extract of Curcuma zedoary according to claim 2, characterized in that, The silica gel chromatographic column has a pore size of 200-300 mesh; the eluent is 80% ethanol; and the preparation method of the zedoary extract further comprises concentrating the eluent until no ethanol is present.

4. The hair loss prevention composition containing the extract of Curcuma zedoary according to claim 1, wherein The minoxidil analogues comprise one or both of a diaminopyrimidine oxide and a pyrrolidinyl diaminopyrimidine oxide.

5. The hair loss prevention composition containing the extract of Curcuma zedoary according to claim 1, wherein The tea extract comprises at least one of a tea leaf extract, a tea seed extract and a camellia seed extract.

6. The hair loss prevention composition containing the extract of Curcuma zedoary according to claim 1, wherein The anti-hair loss composition further comprises a solubilizing agent, the mass ratio of the solubilizing agent to the zedoary extract is (1-10):1, and the solubilizing agent comprises one or more of PEG-40 hydrogenated castor oil, Tween-28, Tween-60, Tween-80, PEG-55 propylene glycol oleate and a polyhydric alcohol.

7. The hair loss prevention composition containing the extract of Curcuma zedoary according to claim 6, characterized in that, The anti-hair loss composition further comprises a solvent, the solvent comprises water, and the added weight of the solvent is 20-35% of the total mass of the zedoary extract, the minoxidil analogues, the tea extract and the solubilizing agent.

8. A method for preparing the hair loss preventing composition containing the extract of Curcuma zedoaria Rosc according to any one of claims 1 to 7, characterized by, The method comprises the following steps: S1, weighing the formula amount of the zedoary extract, the minoxidil analogues, the tea extract and the solubilizing agent for standby; S2, placing the formula amount of the zedoary extract in the solubilizing agent, heating to 40-60°C, homogenizing to prepare a mixture A; S3, adding the formula amount of the minoxidil analogues to the mixture A obtained in step S2, incubating at 40-60°C, homogenizing to prepare a mixture B; S4, after the mixture B in step S3 is cooled to below 40°C, adding the formula amount of the tea extract and the solvent, homogenizing to obtain the anti-hair loss composition.

9. The preparation method according to claim 8, characterized in that, The homogenization speed in steps S2, S3 and S4 is 1500-4000 rpm, and the homogenization time is 5-10 min.

10. Use of the anti-hair loss composition containing zedoary extract according to any one of claims 1-7 in the preparation of a head care product.

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