Method for synergistically enhancing bank slope vegetation water quality purification through arbuscular mycorrhiza and nitrifying bacteria
By introducing a symbiotic system of arbuscular mycorrhizae and nitrifying bacteria into the slope vegetation, the problem of insufficient rhizosphere microenvironment regulation is solved, achieving efficient and stable water purification effect, which is suitable for a variety of water purification scenarios.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- CHINA INST OF WATER RESOURCES & HYDROPOWER RES
- Filing Date
- 2025-12-26
- Publication Date
- 2026-04-14
AI Technical Summary
Existing water purification technologies using riparian vegetation suffer from insufficient regulation of rhizosphere microhabitats, weak synergistic effects, and poor ecological adaptability, leading to unstable purification effects, especially functional decline under barren or stressed environments.
By constructing a symbiotic system of arbuscular mycorrhizae and nitrifying bacteria, a compound microbial agent is formed and inoculated into the plant rhizosphere, thereby creating an expanded rhizosphere microenvironment, optimizing the abundance and activity of functional microorganisms, and enhancing the ability to remove pollutants.
It significantly improves the removal rate of pollutants such as nitrogen and phosphorus by slope vegetation, enhances the stability and resilience of the system, reduces long-term maintenance costs, and is suitable for various water purification scenarios.
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Figure CN121850210A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of water environment ecological governance and ecological engineering technology, specifically involving a method for synergistic regulation of plant rhizosphere microenvironment by arbuscular mycorrhizal and nitrifying bacteria communities to enhance the purification function of riparian vegetation systems for surface runoff polluted water. Background Technology
[0002] The information disclosed in this background section is intended only to enhance understanding of the overall background of the invention and is not necessarily to be construed as an admission or in any way implying that such information constitutes prior art known to those skilled in the art.
[0003] With the acceleration of urbanization and the increasing prominence of non-point source pollution, river and lake slopes, as ecological transition zones between land and water, play a crucial role in intercepting, absorbing, and transforming nutrients such as nitrogen and phosphorus, heavy metals, and organic pollutants in surface runoff. They serve as a natural barrier to control eutrophication. Currently, technologies to enhance the water purification function of riverbank vegetation mainly include: (1) optimizing vegetation configuration by screening and combining plants with strong pollutant absorption capacity; (2) extending hydraulic retention time and promoting physical sedimentation and biological absorption through engineering measures such as constructing vegetation buffer zones and ecological filter ditches; and (3) improving the substrate by adding adsorbent materials such as zeolite and biochar to the soil to enhance the fixation capacity of pollutants. However, the above traditional methods have limitations in terms of purification efficiency, optimization and control, and stability. For example, the absorption efficiency of plants for pollutants is limited by the degree of root development and nutrient absorption capacity. Especially in barren or stressed riverbank habitats, plant growth is poor, resulting in limited and unstable purification effects. Furthermore, riverbank habitats are susceptible to stresses such as drought and flooding, and traditional vegetation systems are prone to functional decline under adverse conditions, resulting in insufficient purification stability. The water purification function of riverbank vegetation largely depends on its rhizosphere, a "microreactor." The rhizosphere is a hotspot for interactions between plants and soil microorganisms, but current technologies often neglect the active introduction and regulation of key beneficial microbial communities in the rhizosphere, failing to fully utilize the synergistic purification potential of plants and microorganisms, leading to ineffective regulation of the rhizosphere microenvironment. Summary of the Invention
[0004] To address the shortcomings of existing technologies, the present invention aims to overcome the deficiencies of insufficient rhizosphere microhabitat regulation, weak synergistic effects, and poor ecological adaptability in existing bank slope vegetation water purification technologies. It provides a method to enhance the water purification effect of bank slope vegetation by synergistically regulating the rhizosphere microhabitat using arbuscular mycorrhizae and nitrifying bacteria. By constructing a symbiotic system between arbuscular mycorrhizae, nitrifying bacteria, and bank slope vegetation, the structure of the rhizosphere microhabitat is optimized, and the abundance and activity of functional microorganisms are increased. This enhances the vegetation's ability to remove pollutants such as nitrogen, phosphorus, and organic matter from water, achieving the dual goals of bank slope ecological restoration and water purification.
[0005] This invention proposes to actively intervene in the rhizosphere microenvironment of riverbank vegetation by artificially inoculating an agent formed by arbuscular mycorrhizal and nitrifying bacteria communities. By utilizing the symbiotic system formed by the arbuscular mycorrhizal-nitrifying bacteria complex community and plants, an expanded and functionally enhanced "root-rhizosphere microenvironment-mycelial" composite purification micro-ecosystem is constructed. The aim is to systematically improve the water purification capacity of riverbank vegetation and enhance the long-term operational stability and ecological resilience of the filter zone.
[0006] This invention provides the following technical solutions:
[0007] In a first aspect, this invention provides a method for enhancing the water purification effect of riverbank vegetation by synergistically regulating the rhizosphere microenvironment using arbuscular mycorrhizae and nitrifying bacteria, the method comprising the following steps:
[0008] S1: Prepare an immobilized composite microbial agent containing one or more active arbuscular mycorrhizal fungi and nitrifying bacteria;
[0009] S2: During the root colonization stage of the target slope plants, an inoculum made of immobilized compound microbial agent is applied to the plant rhizosphere, so that arbuscular mycorrhizae, nitrifying bacteria and plant roots form a symbiotic relationship.
[0010] S3: By regulating the rhizosphere microenvironment through the symbiotic system, a composite purification system composed of plant roots, arbuscular mycorrhizal fungal hyphae network, nitrifying bacteria and soil microbial community is constructed to enhance the removal of pollutants in water.
[0011] Preferably, the preparation of the immobilized compound inoculant for arbuscular mycorrhizal and nitrifying bacteria includes:
[0012] (1) Selection of fungal species: Select arbuscular mycorrhizal single or mixed species that are suitable for the target slope habitat (such as moisture-tolerant, flood-tolerant, and barren-tolerant) and have strong affinity with the target plants.
[0013] (2) Carrier selection and inoculum preparation: The spores, mycelial segments, and nitrifying bacteria of arbuscular mycorrhizae are mixed with a sterile carrier to prepare an inoculum that is easy to store and apply.
[0014] Preferably, the microbial strains include one or more mixed strains of endophytic rhizomycetes and surface arbuscular mycorrhizal fungi. A compound microbial agent composed of multiple functionally complementary arbuscular mycorrhizae is preferred. The nitrifying bacteria are preferably nitrifying bacteria that have been acclimated to the nitrification tanks of wastewater treatment plants over a long period.
[0015] Preferably, the sterile carrier is one or more of river sand, vermiculite, and peat moss.
[0016] Preferably, each gram of inoculum contains no less than 50-200 live spores, and the optimal inoculum amount of nitrifying bacteria is between 1% and 5%.
[0017] Preferably, in S2, the method for constructing the rhizosphere microhabitat of the bank slope vegetation is as follows:
[0018] (1) Target vegetation selection: Select native riparian plants with well-developed root systems, strong water purification capabilities (such as strong ability to accumulate nitrogen, phosphorus and heavy metals) and easy to form good symbiosis with arbuscular mycorrhizae. For example, shade-tolerant and moisture-tolerant species such as ryegrass, reed, white clover and liriope.
[0019] (2) Construction of vegetation rhizosphere microhabitat: This is carried out during the seedling stage or transplanting. The immobilized arbuscular mycorrhizal-nitrifying bacteria composite inoculant prepared in the previous step is evenly mixed into the substrate of the seedling trays or nutrient pots at a volume of 3%-10%. When transplanting the seedlings into the planting holes on the bank slope, the surrounding substrate is filled and gently compacted to ensure full contact between the inoculant and the roots. Through appropriate initial water management, rapid infection of the plant roots by arbuscular mycorrhizae and rapid colonization of the nitrifying bacteria community are promoted, establishing a stable mycorrhizal-nitrifying bacteria-vegetation symbiotic system.
[0020] Preferably, step S3 further includes a method for synergistic regulation of system stability and purification function, as follows:
[0021] (1) Dynamic regulation of rhizosphere microenvironment:
[0022] Water management: After the stable symbiotic system of arbuscular mycorrhizal fungi, nitrifying bacteria, and plants is established, maintain the soil moisture content of the bank slope at 70%-80% of field capacity for the first 1-2 weeks to promote arbuscular mycorrhizal infection and colonization of nitrifying bacteria. Later, based on the vegetation's growth needs, maintain the soil moisture content at 50%-60% of field capacity (for soil-type bank slopes) or regularly replenish water through a drip irrigation system (for stony / saline-alkali bank slopes) to avoid waterlogging that could lead to root rot.
[0023] Nutrient regulation: In the early stage of plant growth, slow-release organic carbon sources (such as biochar, lignin, decomposed soybean meal, etc.) can be applied to promote the reproduction of arbuscular mycorrhizae and nitrifying bacteria. The application amount should be controlled at 50-200 grams per square meter, and it should be applied in a shallow layer around the plant rhizosphere.
[0024] Microbial community stability control: During system operation, avoid using high doses of chemical fungicides and fast-acting phosphate fertilizers to protect the rhizosphere microenvironment and functional microbial activity. Regularly check the vegetation growth and mycorrhizal condition, and replant or supplement inoculate as necessary.
[0025] (2) Optimization and regulation of purification function: Water samples were collected monthly from near the bank slope to test water quality indicators such as total nitrogen, total phosphorus, chemical oxygen demand, and ammonia nitrogen. At the same time, rhizosphere soil samples were collected to determine the arbuscular mycorrhizal retention rate and rhizosphere enzyme activities (urease, phosphatase, sucrase) to determine the water purification effect and microbial activity. Arbuscular mycorrhizal-nitrifying bacteria compound inoculant was applied to the rhizosphere soil every 3-6 months at a dosage of 5-10g per plant to further optimize the structure and stability of the rhizosphere functional microbial community.
[0026] In a second aspect, the present invention also provides a system for enhancing water quality purification by riparian vegetation. The system is constructed by the above-described method, and its core includes an arbuscular mycorrhizal-nitrifying bacteria complex that forms a symbiotic relationship with the target riparian vegetation, and a rhizosphere microenvironment rich in functional microorganisms that is regulated by the symbiotic relationship.
[0027] In the above technical solution, this invention addresses the limited interception capacity of riverbank vegetation for pollutants in water by utilizing arbuscular mycorrhizae to regulate rhizosphere microorganisms and enhance the water purification effect on riverbanks. In a particularly effective embodiment, *Rhizocystis ulmoides* is used as a typical representative of arbuscular mycorrhizae, and ryegrass is used as the native vegetation to construct a symbiotic microenvironment of arbuscular mycorrhizae coupled with nitrifying bacteria and a vegetation filter zone. The mycelial network of arbuscular mycorrhizae expands the absorption interface, enhances nitrifying bacteria colonization, simultaneously strengthens the conversion and fixation of nitrogen and phosphorus, and enriches functional microorganisms, forming a synergistic water purification system of "vegetation-arbuscular mycorrhizae / nitrifying bacteria-rhizosphere microorganisms." This method significantly improves purification efficiency and system stability, providing an efficient and sustainable solution for riverbank ecological restoration and non-point source pollution control.
[0028] The method for enhancing the water purification effect of riverbank vegetation by regulating the rhizosphere microenvironment through arbuscular mycorrhizal coupling with nitrifying bacteria provided by this invention has the following advantages compared with existing riverbank vegetation water purification technologies:
[0029] (1) Significantly improve purification efficiency: This invention fundamentally improves the plant’s ability and efficiency to absorb pollutants by introducing arbuscular mycorrhizae and nitrifying bacteria. The mycelial network greatly expands the absorption range of the root system, increases the contact area and residence time between pollutants and the purification system, regulates the activity of rhizosphere enzymes through arbuscular mycorrhizae (such as increasing urease activity by 30%-50% and phosphatase activity by 25%-40%), and enhances the rhizosphere nitrogen metabolism activity through nitrifying bacteria, promotes nitrogen and phosphorus conversion and activates insoluble phosphorus in the soil. The removal rate of nitrogen and phosphorus is expected to be increased by more than 30%.
[0030] (2) Targeted regulation of the rhizosphere microenvironment to achieve functional enhancement: The present invention actively introduces a complex community of arbuscular mycorrhizae and nitrifying bacteria. Its metabolic activities change the composition of rhizosphere secretions and enrich specific microbial communities with denitrification and phosphorus removal functions (such as denitrifying bacteria and polyphosphate-accumulating bacteria). It directionally reconstructs a rhizosphere microenvironment that is conducive to the transformation and degradation of pollutants, forming a synergistic purification microbial network with arbuscular mycorrhizae as the "hub".
[0031] (3) Enhance system stability and stress resistance: Arbuscular mycorrhizae can improve the water and nutrient status of plants and enhance their survival ability under stress environments such as barren slopes and drought. At the same time, the secretions of mycelia, such as related proteins and polysaccharides, help to aggregate soil particles and enhance the slope's resistance to erosion. The special structure of arbuscular mycorrhizae helps to rapidly colonize and enrich nitrifying bacteria, making the purification system more stable and durable.
[0032] (4) Environmentally friendly and cost-effective: This invention is a pure biological ecological technology with no secondary pollution. Once established, the system can be self-sustaining and play a long-term purification role, greatly reducing the long-term cost of bank slope ecological maintenance. The arbuscular mycorrhizal-nitrifying bacteria compound agent used can be produced on a large scale, with low cost, simple construction, and low operation and maintenance costs, and can be widely promoted and applied.
[0033] (5) Synergy and universality: This method can be combined with existing vegetation configuration optimization and ecological engineering measures to produce synergistic effects. It is applicable to various types of river and lake banks, wetlands, rain gardens and other scenarios that require enhanced water purification, and has a wide range of applications.
[0034] The specific technical solution of this invention includes three steps: selection of functional arbuscular mycorrhizal fungi and preparation of immobilized composite microbial agents, construction of rhizosphere microenvironment for riverbank vegetation, and synergistic regulation of system stability and purification function. Suitable functional arbuscular mycorrhizal fungi and nitrifying bacteria are selected to form a composite microbial community, constructing a symbiotic system between the composite microbial community and plant roots. This provides carbon sources and a microenvironment for rhizosphere microorganisms, enhancing the adsorption and transformation capacity of plant roots for pollutants. By optimizing the functional microbial community structure through arbuscular mycorrhizal-nitrifying bacteria communities, rhizosphere enzyme activity is enhanced, and nitrogen and phosphorus nutrient conversion is promoted, achieving synergistic enhancement of water purification through "vegetation-arbuscular mycorrhizal / nitrifying bacteria-rhizosphere microorganisms". Attached Figure Description
[0035] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an improper limitation of the invention.
[0036] Figure 1 A schematic diagram illustrating the effect of arbuscular mycorrhizal / nitrifying bacteria on the removal of total nitrogen and total phosphorus by enhanced slope vegetation;
[0037] Figure 2 A schematic diagram illustrating how arbuscular mycorrhizal fungi / nitrifying bacteria improve soil enzyme activity in riparian vegetation.
[0038] Figure 3 A schematic diagram illustrating how arbuscular mycorrhizal fungi / nitrifying bacteria regulate root growth in riverbank vegetation. Detailed Implementation
[0039] It should be noted that the following detailed description is illustrative and intended to provide further explanation of the invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.
[0040] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of exemplary embodiments according to the invention. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.
[0041] Example
[0042] An example of a method to enhance the water purification function of soil slope vegetation in a river by using arbuscular mycorrhizal fungi was carried out, with an implementation plan for the slope water flow intensity and water quality in North China.
[0043] It is carried out in the following steps:
[0044] Preparation of Arbuscular Mycorrhizal-Nitrifying Bacteria Immobilized Composite Inoculum: *Rhizocystis* fungi were selected as the arbuscular mycorrhizae. Peat moss and activated carbon were mixed at a volume ratio of 3:1 and sterilized by high-temperature steam at 121℃ for 2 hours to serve as a sterile carrier. Activated sludge from an aerobic tank of a municipal wastewater treatment plant was selected as the nitrifying bacteria, and the bacteria were rinsed three times with sterile water. The original inoculum was thoroughly mixed with the sterilized carrier. Spore counting was performed using a hemocytometer under a microscope, and the final inoculum concentration was precisely adjusted to 150 ± 20 viable spores per gram. Then, 2% (by volume) of the nitrifying bacteria was added. The prepared composite inoculum was stored at 4℃ in a cool, dark place for later use.
[0045] Construction of rhizosphere microhabitats for riverbank vegetation: *Liriope muscari*, a native plant with strong purification capabilities, was selected for cultivation. Seedlings were raised in plug trays. The prepared immobilized compound microbial agent was evenly mixed into the nutrient soil (garden soil:vermiculite = 2:1) at 5% of the soil volume. When the seedlings reached 10-15 cm in height, they were transplanted on a cloudy day in spring. Planting holes were dug on the riverbank at 50 cm × 50 cm intervals. In each planting hole, a small amount of soil mixed with the microbial agent (approximately 20 grams per hole) was placed first, followed by the seedling, ensuring close contact between the roots and the compound microbial agent. Finally, the planting hole was filled with soil and gently compacted. For one week after transplanting, the soil moisture content in the rhizosphere was maintained at approximately 75% of field capacity. Watering was applied appropriately every evening to ensure seedling survival and create optimal humidity conditions for arbuscular mycorrhizal fungal infection and nitrifying bacteria colonization.
[0046] System stability and purification functions were synergistically regulated: Two weeks after transplanting, seedlings were confirmed to have survived. The irrigation strategy was adjusted to maintain soil moisture content between 55% and 65% of field capacity, simulating natural rainfall and avoiding over-irrigation. In the fourth week after transplanting, to promote the development of mycorrhizae, nitrifying bacteria, and mycelial microorganisms, 100 grams of granular biochar were evenly spread per square meter around the plant rhizosphere and shallowly raked into the soil. No chemical fungicides were used throughout the growing season, and the application of fast-acting phosphate fertilizer was controlled.
[0047] After three months of system operation, water samples were collected from the inlet and outlet of the riverbank. Test results showed that the total nitrogen (TN) and total phosphorus (TP) concentrations at the outlet were reduced by an average of 35% and 40% compared to the inlet. Simultaneously, the activities of rhizosphere soil phosphatase and urease increased by 50% and 30%, respectively, compared to the uninoculated control area. Based on the monitoring results, functional microbial agents were supplemented. After six months of system operation, 5 grams of immobilized compound microbial agent were applied to the rhizosphere of each plant to further optimize the rhizosphere microbial community structure and enhance nitrogen and phosphorus removal functions.
[0048] The method described in this embodiment successfully constructed a stable synergistic purification system of "Liriope muscari - Rhizocystis radicans / nitrifying bacteria - soil microorganisms". Compared with the control area planted with only the same vegetation but not inoculated with the compound microbial agent, this embodiment showed:
[0049] (1) Vegetation growth advantage: The plant height, biomass and root development were significantly better than the control group, with the total root length increasing by 46% and the fine root length increasing by 54%.
[0050] (2) Improved soil physical and chemical properties: The porosity of the rhizosphere soil increased significantly by more than 59% compared with the control group.
[0051] (3) Improved water purification efficiency: The removal rate of TN and TP is 25%-40% higher than that of the control group on average.
[0052] (4) Enhanced system stability: After experiencing a short-term drought in summer, the vegetation in the inoculated area recovered much faster than that in the control group, showing stronger resistance.
[0053] This embodiment fully demonstrates that the technical solution provided by the present invention can effectively construct a high-efficiency and stable bank slope vegetation purification system, significantly enhance its water purification effect, and achieve the expected invention objective.
[0054] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.
Claims
1. A method for enhancing the water purification effect of bank slope vegetation by synergistically regulating the rhizosphere microenvironment using arbuscular mycorrhizae and nitrifying bacteria, the method comprising the following steps: S1: Prepare an immobilized composite microbial agent containing one or more active arbuscular mycorrhizal fungi and nitrifying bacteria; S2: During the root colonization stage of the target slope plants, an inoculum made of immobilized compound microbial agent is applied to the plant rhizosphere, so that arbuscular mycorrhizae, nitrifying bacteria and plant roots form a symbiotic relationship. S3: By regulating the rhizosphere microenvironment through the symbiotic system, a composite purification system composed of plant roots, arbuscular mycorrhizal fungal hyphae network, nitrifying bacteria and soil microbial community is constructed to enhance the removal of pollutants in water.
2. The method as described in claim 1, characterized in that, The preparation process of immobilized compound inoculants containing arbuscular mycorrhizal fungi and nitrifying bacteria includes: (1) Selection of fungal strains: Select arbuscular mycorrhizal single or mixed strains that are suitable for the target slope habitat and have strong affinity with the target plants; (2) Carrier selection and inoculum preparation: The spores, mycelial segments, and nitrifying bacteria of arbuscular mycorrhizae are mixed with a sterile carrier to prepare an inoculum that is easy to store and apply.
3. The method as described in claim 2, characterized in that, The microbial strains include one or more mixed strains of Rhizocystis endophyticus and Rhizocystis terrestrialus. The nitrifying bacteria are nitrifying bacteria that have been domesticated in the nitrification tank of the sewage treatment plant for a long time. The sterile carrier is one or more of river sand, vermiculite, and peat moss.
4. The method as described in claim 2, characterized in that, Each gram of inoculum contains no less than 50-200 live spores, and the optimal inoculum amount for nitrifying bacteria is between 1% and 5%.
5. The method as described in claim 1, characterized in that, In step S2, the target slope plants are native plants with a strong ability to absorb nitrogen and phosphorus pollutants, and the root colonization stage includes the seedling stage or the transplanting stage.
6. The method as described in claim 5, characterized in that, When inoculating during the seedling stage, the immobilized compound inoculant is evenly mixed into the seedling substrate at a volume ratio of 5%-10%. When inoculating during transplanting, 10-20 grams of the inoculant is applied around the roots of the seedlings in the transplanting hole.
7. The method according to claim 1, characterized in that, After step S2 and before step S3, the process also includes applying a slow-release organic carbon source around the plant rhizosphere.
8. The method according to claim 1, characterized in that, In step S3, the pollutants include nitrogen and phosphorus nutrients and / or organic pollutants; the enhanced removal of pollutants is achieved through at least one of the following pathways: direct absorption by arbuscular mycorrhizal fungal hyphae, metabolic activity of nitrifying bacteria, activation of soil phosphorus to promote plant absorption, and enrichment of mycelial microbial communities to promote nitrogen and phosphorus transformation and organic matter degradation.
9. The method according to claim 1, characterized in that, Step S3 also includes a method for synergistic regulation of system stability and purification functions, the specific steps of which are as follows: (1) Dynamic regulation of rhizosphere microenvironment: Water regulation: After the stable symbiotic system of arbuscular mycorrhizal fungi-nitrifying bacteria and plants is established, the soil moisture content of the bank slope should be maintained at 70%-80% of field capacity in the first 1-2 weeks to promote arbuscular mycorrhizal infection and colonization of nitrifying bacteria. In the later stage, the soil moisture content should be maintained at 50%-60% of field capacity (soil bank slope) or water should be replenished regularly through drip irrigation system (rocky / saline bank slope) to avoid water accumulation that could lead to root rot. Nutrient regulation: In the early stage of plant growth, slow-release organic carbon sources can be applied to promote the reproduction of arbuscular mycorrhizae and nitrifying bacteria. The application rate should be controlled at 50-200 grams per square meter, and it should be applied in a shallow layer around the plant roots. Microbial community stability control: During system operation, avoid using high doses of chemical fungicides and fast-acting phosphate fertilizers to protect the rhizosphere microenvironment and functional microbial activity. Regularly check the vegetation growth and mycorrhizal condition, and replant or supplement inoculate as necessary. (2) Optimization and regulation of purification function: Water samples near the bank slope are collected monthly to test water quality indicators such as total nitrogen, total phosphorus, chemical oxygen demand, and ammonia nitrogen. At the same time, rhizosphere soil samples are collected to determine the arbuscular mycorrhizal retention rate and rhizosphere enzyme activity to determine the water purification effect and microbial activity. Arbuscular mycorrhizal-nitrifying bacteria compound agent is applied to the rhizosphere soil every 3-6 months at a dosage of 5-10 g per plant to further optimize the structure and stability of the rhizosphere functional microbial community.
10. A system for enhancing water purification through riverbank vegetation, characterized in that, The system is constructed by any one of claims 1 to 9, and its core includes an arbuscular mycorrhizal-nitrifying bacteria complex that forms a symbiotic relationship with the target slope plants, and a rhizosphere microenvironment rich in functional microorganisms regulated by the symbiotic relationship.