Cell culture medium storing and subpackaging device

Abstract

The invention discloses a cell culture medium storing and subpackaging device which comprises a base, a supporting column fixedly connected to the upper end of the base, a limiting rail fixedly connected to the upper end of the supporting column, a connecting frame connected to the limiting rail, a fixing sleeve connected to the connecting frame, a subpackaging disc connected to the limiting rail, and a containing groove formed in the subpackaging disc. The lower end of the split charging disc is fixedly connected with a rotating rod, the lower end of the rotating rod is connected with a rotating umbrella, the upper end of the base is connected with a center base, the bottom end of the rotating umbrella is rotationally connected to the center base, and the fixing sleeve is connected with a split charging needle tube. The culture dish on the split charging disc is subjected to culture solution split charging through the split charging needle tube, during split charging, the culture dish and the culture solution adding structure do not need to be frequently moved, the split charging process is stably carried out, the culture dish is jacked through the supporting suction cup structure, the culture dish is made to be close to the split charging needle tube, the phenomenon that the culture solution is splashed out during adding is avoided, and the split charging efficiency is improved. And the subpackaging stability is further improved.

Description

Technical Field

[0001] This invention relates to the field of cell culture medium dispensing technology, and more particularly to a cell culture medium storage and dispensing device. Background Technology

[0002] Cell culture medium is not only the basic substance that supplies nutrients to cultured cells and promotes cell reproduction and proliferation, but also the living environment for the growth and reproduction of cultured cells.

[0003] Before cell culture and storage, the culture medium needs to be aliquoted. To ensure a consistent culture environment in the culture dishes, the same component of culture medium needs to be added to each dish to form the same culture medium. The amount of culture medium added also needs to be consistent. The traditional method of adding the medium is to do so manually using a pipette. During the addition process, the pipette needs to be moved back and forth between the culture dish and the culture medium, which increases the probability of contamination of the culture medium. Moreover, when dispensing a large number of culture dishes, the operator's arm may shake, causing excess culture medium to enter the culture dish. This results in a certain difference in the amount of liquid dispensed into each culture dish, which affects the culture environment of subsequent cell engineering projects and leads to errors in cell culture data.

[0004] Based on this, a cell culture medium storage and dispensing device is proposed. Summary of the Invention

[0005] The purpose of this invention is to provide a cell culture medium storage and dispensing device in order to solve the above-mentioned problems.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: A cell culture medium storage and dispensing device includes a base, a support column fixedly connected to the upper end of the base, a limiting rail fixedly connected to the upper end of the support column, a connecting frame connected to the limiting rail, and a fixing sleeve connected to the connecting frame. A dispensing tray is connected to the limiting rail, and a placement slot is provided on the dispensing tray. A rotating rod is fixedly connected to the lower end of the dispensing tray, and a rotating umbrella is connected to the lower end of the rotating rod. A central seat is connected to the upper end of the base, and the bottom end of the rotating umbrella is rotatably connected to the central seat. The fixed sleeve is connected to a dispensing needle tube, the dispensing needle tube is connected to a connecting tube, the connecting tube is connected to an external connecting tube and an injection tube, the injection tube is connected to a pull rod, and one end of the pull rod is fixedly connected to an abutment piece. A flipping block is provided below the abutment piece. One end of the flipping block is threaded with a screw, and the other end is fixedly connected with a hook. A sliding column is fixedly connected to the flipping block, and a gear is fixedly connected to the outside of the flipping block. A flipping rod is fixedly connected to one side of the gear. A limit post is fixedly connected to the upper end of the base. A lifting rod is slidably connected to the limit post. A lifting block is fixedly connected to the upper end of the lifting rod. The flipping rod is rotatably connected to the lifting block. A transmission mechanism for driving the lifting block to rise and fall is provided at the upper end of the base.

[0007] Preferably, the transmission mechanism includes a deflection frame, with rotating tubes connected to both ends of the deflection frame, and a contact rod connected to the rotating tube. A transmission rod is connected to the outer side of another rotating tube. A hinge frame one and a hinge frame three are fixedly connected to the upper end of the base. A driving member for driving the contact rod to deflect is provided on one side of the contact rod. A toggle member for moving the flipping rod is connected to the transmission rod.

[0008] Preferably, the driving component includes a crank handle, and a rotating seat one and a rotating seat two are fixedly connected to the upper end of the base. One end of the crank handle is connected to a limit ring and a cam.

[0009] Preferably, one end of the crank handle is connected to a semi-cylinder, and a toggle post is connected to the semi-cylinder. The semi-cylinder and the toggle post are connected in a cooperative manner with the rotating umbrella.

[0010] Preferably, the actuating component includes a lifting rod, a second hinge frame is fixedly connected to the upper end of the base, one end of the lifting rod is rotatably connected to the second hinge frame, the lifting rod is connected to the transmission rod through a connecting buckle, one end of the lifting rod is provided with a sliding groove, and one end of the flipping rod is slidably connected to the sliding groove.

[0011] Preferably, a support suction cup is fixedly connected to the upper end of the lifting block.

[0012] Preferably, a connecting block is fixedly connected to the upper end of the base, a fixing frame is connected to the connecting block, and a rack is connected to the fixing frame.

[0013] Preferably, the upper and lower ends of the fixed frame are connected to limit frames, the limit frames and the sliding column cooperate with each other, and the rack and the gear cooperate with each other.

[0014] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are: 1. This application adopts a dispensing syringe structure to dispense culture medium into culture dishes on a dispensing tray. During dispensing, the culture dish and the culture medium addition structure do not need to be moved frequently, and the dispensing process is stable. Furthermore, the culture dish is lifted by the support suction cup structure, which brings the culture dish and the dispensing syringe closer together, avoiding splashing of culture medium during addition and further improving the stability of dispensing.

[0015] 2. This application employs a rotating umbrella structure, utilizing the interaction between the rotating umbrella, the semi-cylinder, and the actuating column. When the rotating umbrella and the semi-cylinder are engaged, the dispensing tray stops rotating when the handle is rotated, ensuring the stability of the structure when adding culture medium to the dispensing syringe. When the rotating umbrella and the actuating column are engaged, rotating the handle will drive the dispensing tray to rotate, at which point the culture dish located below the dispensing syringe is dispensed. The rotation of the dispensing tray moves the next culture dish to below the dispensing syringe, and the flipping block ensures that the amount of culture medium added through the dispensing syringe is the same each time. Attached Figure Description

[0016] Figure 1 A schematic diagram of the overall structure of the dispensing device provided according to an embodiment of the present invention is shown; Figure 2 A schematic diagram of the structure of the other side of the dispensing device provided according to an embodiment of the present invention is shown; Figure 3 An exploded structural diagram of the dispensing tray connection provided according to an embodiment of the present invention is shown; Figure 4 A schematic diagram of the structure of the limiting rail connection provided according to an embodiment of the present invention is shown; Figure 5 An exploded structural diagram of the semi-cylindrical connection provided according to an embodiment of the present invention is shown; Figure 6 An exploded structural diagram of the abutment rod connection provided according to an embodiment of the present invention is shown; Figure 7 An exploded view of the connection point of the connector buckle provided according to an embodiment of the present invention is shown; Figure 8 A schematic diagram of the lifting rod connection provided according to an embodiment of the present invention is shown; Figure 9 An exploded structural diagram of the flip rod connection provided according to an embodiment of the present invention is shown.

[0017] Legend: 1. Base; 2. Support column; 3. Dispensing tray; 4. Placement slot; 5. Connecting frame; 6. Fixing sleeve; 7. Dispensing needle; 8. Connecting tube; 9. External connecting tube; 10. Injection tube; 11. Pull rod; 12. Abutment plate; 13. Rotating seat one; 14. Rotating seat two; 15. Hinge frame one; 16. Hinge frame two; 17. Handle; 18. Support suction cup; 19. Connecting buckle; 20. Limiting column; 21. Connecting block; 22. Flipping block; 23. Hook; 24. Rotary... 25. Rotating umbrella; 26. Center seat; 27. Limiting rail; 28. Rotating rod; 29. ​​Limiting ring; 30. Flipping rod; 31. Hinge frame three; 32. Fixed frame; 33. Cam; 34. Semi-cylinder; 35. Actuating column; 36. Deflecting frame; 37. Rotating tube; 38. Abutting rod; 39. Transmission rod; 40. Lifting rod; 41. Slide groove; 42. Lifting block; 43. Gear; 44. Sliding column; 45. Lifting rod; 46. Limiting frame; 47. Rack. Detailed Implementation

[0018] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0019] Please see Figure 1-9 The present invention provides a technical solution: A cell culture medium storage and dispensing device includes a base 1, a support column 2 fixedly connected to the upper end of the base 1, a limiting rail 26 fixedly connected to the upper end of the support column 2, two limiting rails 26 are provided, the two limiting rails 26 are connected to each other through a connecting frame 5, the connecting frame 5 is connected to the limiting rail 26, and the fixing sleeve 6 is connected to the connecting frame 5. The fixing sleeve 6 is fitted on the outside of the dispensing needle tube 7 to ensure the firmness of the connection of the dispensing needle tube 7. A dispensing tray 3 is connected to the limiting rail 26. The dispensing tray 3 is rotatably connected to the limiting rail 26. The dispensing tray 3 has a placement groove 4. Multiple placement grooves 4 are evenly distributed on the dispensing tray 3. The placement grooves 4 are mainly used to place culture dishes. By dispensing culture medium into the culture dishes, a culture medium structure is formed. A rotating rod 27 is fixedly connected to the lower end of the dispensing tray 3. A rotating umbrella 24 is connected to the lower end of the rotating rod 27. The rotating umbrella 24 has multiple semi-circular grooves and multiple strip grooves. The semi-circular grooves and strip grooves are alternately arranged. A central seat 25 is connected to the upper end of the base 1. The bottom end of the rotating umbrella 24 is rotatably connected to the central seat 25. The central seat 25 is used to improve the stability of the rotating umbrella 24 and the rotating rod 27 rotating on it. A dispensing syringe 7 is connected to the fixed sleeve 6. The lower end of the dispensing syringe 7 has a single-pass structure, meaning that the culture medium can only be injected into the culture dish from below the dispensing syringe 7, and air cannot be drawn in from below the dispensing syringe 7. A connecting tube 8 is connected to the dispensing syringe 7. An external connecting tube 9 and an injection tube 10 are connected to the connecting tube 8. The external connecting tube 9 is used to connect the pipeline for delivering the culture medium. A lever 11 is connected to the injection tube 10. The upper end of the lever 11 passes through the injection tube 10 and is connected to a piston structure. By pushing the lever 11, the piston moves and the culture medium is injected into the culture dish to form a culture medium. A contact plate 12 is fixedly connected to one end of the lever 11. The external structure moves the piston by pushing or pulling the contact plate 12. A flipping block 22 is provided below the abutment plate 12. One end of the flipping block 22 is threadedly connected to a screw 44. The screw 44 and the flipping block 22 are threadedly connected. By rotating the screw 44, the distance between one end of the screw 44 and one end of the flipping block 22 can be changed. The other end of the flipping block 22 is fixedly connected to a hook 23. By using the hook 23 to fasten to the abutment plate 12 and move it, the abutment plate 12 can be pulled back to its initial position, ensuring that the amount of culture medium added to the culture dish is the same each time. A sliding column 43 is fixedly connected to the flipping block 22. The sliding column 43 is mainly used to limit the position of the flipping block 22 before and after flipping. The sliding column 43 can slide on the limiting frame 46. A gear 42 is fixedly connected to the outside of the base 22. A flipping rod 29 is fixedly connected to one side of the gear 42. One end of the flipping rod 29 is connected to the center of the gear 42, and the flipping rod 29 rotates as the gear 42 rotates. A limit post 20 is fixedly connected to the upper end of the base 1. A lifting rod 45 is slidably connected to the limit post 20. There are two lifting rods 45. The two lifting rods 45 limit the structure of the lifting block 41, so that the lifting block 41 can only move vertically. The lifting block 41 is fixedly connected to the upper end of the lifting rod 45. The flipping rod 29 is rotatably connected to the lifting block 41. A transmission mechanism for driving the lifting block 41 to move up and down is provided at the upper end of the base 1.

[0020] Specifically, such as Figure 9 As shown, the transmission mechanism includes a deflection frame 35, with rotating tubes 36 connected to both ends of the deflection frame 35. The two rotating tubes 36 are rotatably connected to the hinge frame 15 and the hinge frame 30, respectively. An abutment rod 37 is connected to the rotating tube 36. One end of the abutment rod 37 abuts against the cam 32. The rotation of the cam 32 causes the abutment rod 37 to deflect. A transmission rod 38 is connected to the outside of the other rotating tube 36. The upper end of the base 1 is fixedly connected to the hinge frame 15 and the hinge frame 30. A driving member is provided on one side of the abutment rod 37 to drive the abutment rod 37 to deflect. A toggle member is connected to the transmission rod 38 to move the flipping rod 29.

[0021] Specifically, such as Figure 1 , Figure 5 and Figure 6 As shown, the driving component includes a crank handle 17, one end of which is rotatably connected to a rotating seat 13 and a rotating seat 2 14. The upper end of the base 1 is fixedly connected to the rotating seat 13 and the rotating seat 2 14. One end of the crank handle 17 is connected to a limit ring 28 and a cam 32. The limit ring 28 is used to ensure that the connection state between the crank handle 17 and the rotating seat 13 remains unchanged, thereby limiting its structure and improving the stability of the crank handle 17 rotation.

[0022] Specifically, such as Figure 5 As shown, a semi-cylinder 33 is connected to one end of the crank handle 17, and an actuating post 34 is connected to the semi-cylinder 33. The semi-cylinder 33 and the actuating post 34 are connected to the rotating umbrella 24. When the semi-cylinder 33 and the rotating umbrella 24 are engaged, the rotating umbrella 24 remains stationary. When the actuating post 34 and the rotating umbrella 24 are engaged, the rotating umbrella 24 rotates.

[0023] Specifically, such as Figure 7 and Figure 8 As shown, the actuating component includes a lifting rod 39. A second hinge frame 16 is fixedly connected to the upper end of the base 1. One end of the lifting rod 39 is rotatably connected to the second hinge frame 16. The lifting rod 39 is connected to the transmission rod 38 through a connecting buckle 19. The two ends of the connecting buckle 19 are rotatably connected to the lifting rod 39 and the transmission rod 38, respectively. A sliding groove 40 is provided at one end of the lifting rod 39. The sliding groove 40 is located at the end of the lifting rod 39 away from the second hinge frame 16. One end of the flipping rod 29 is slidably connected to the sliding groove 40. By changing the deflection angle of the lifting rod 39, that is, changing the position of the sliding groove 40, the position of the flipping rod 29 on the sliding groove 40 is changed.

[0024] Specifically, such as Figure 2 As shown, a support suction cup 18 is fixedly connected to the upper end of the lifting block 41. The support suction cup 18 is used to support the culture dish, so that its height can be increased, while also ensuring the stability of the culture dish structure.

[0025] Specifically, such as Figure 9 As shown, a connecting block 21 is fixedly connected to the upper end of the base 1, and a fixing frame 31 is connected to the connecting block 21. The fixing frame 31 is connected to the connecting block 21 by screws, and a rack 47 is connected to the fixing frame 31. The rack 47 is arranged in the middle part of the fixing frame 31.

[0026] Specifically, such as Figure 9 As shown, the upper and lower ends of the fixed frame 31 are connected to the limit frame 46. There are two limit frames 46, which are located at the upper and lower ends of the fixed frame 31. The limit frame 46 and the sliding column 43 cooperate with each other, and the rack 47 and the gear 42 cooperate with each other.

[0027] In summary, the cell culture medium storage and dispensing device provided in this embodiment allows for the sequential placement of culture dishes into the respective slots 4 on the dispensing tray 3 when cell culture medium needs to be dispensed. The external connecting pipe 9 is then connected to the pipeline for conveying the culture medium. The operator simply needs to turn the crank handle 17, which rotates the cam 32 and the semi-cylinder 33 connected to it. The rotation of the cam 32 causes the abutment rod 37, which abuts against the cam 32, to deflect. This deflection of the abutment rod 37 causes the deflection frame 35 and the transmission rod 38 to deflect. When the transmission rod 38 deflects, the connecting buckle 19 supports the lifting rod 39 to rotate around the hinge frame 16, causing the sliding groove 40 at one end of the lifting rod 39 to move the flipping rod 29 up and down. During the lifting and lowering process, one end of the flipping rod 29 slides within the slide groove 40. At this time, the flipping rod 29 drives the lifting block 41 to move up and down in the vertical direction. The support suction cup 18 at the upper end of the lifting block 41 supports the culture dish located in the placement groove 4, raising its height so that the culture dish structure is close to the dispensing syringe 7, ensuring stable dispensing. The rotating umbrella 24 is provided with a semi-circular groove and a strip groove structure. The semi-circular groove structure fits into the semi-cylinder 33, while the strip groove structure can cooperate with the actuating column 34. When the semi-cylinder 33 and the rotating umbrella 24 are in cooperation, the crank 17 structure rotates, but the rotating umbrella 24 structure does not rotate. This is the time period when the culture medium in the dispensing syringe 7 is added to the culture dish. When the actuating column 34 and the rotating umbrella 24 are in cooperation, the crank 17 structure rotates, but the rotating umbrella 24 structure does not rotate. When they work together, the rotating umbrella 24 is driven to rotate by the actuating column 34. At this time, the dispensing tray 3 rotates. During the time when the culture dish moves to the bottom of the dispensing syringe 7, when the longest diameter of the cam 32 contacts the abutment rod 37, the semi-cylindrical structure 33 is perfectly matched with the semi-circular groove structure on the rotating umbrella 24. At this time, the lifting rod 39 drives the support suction cup 18 to rise to its maximum height through the multi-structure. When the flipping rod 29 rises, it will drive the gear 42 and the rack 47 to engage with each other. That is, when the gear 42 moves from the lowest point to the highest point with the flipping rod 29, the gear 42 does not engage with the rack 47 at first. At this time, the sliding column 43 engages with the lower limiting frame 46. As the gear 42 moves upward, the sliding column 43 slides within the lower limiting frame 46. When gear 43 disengages from the limiting bracket 46, the gear 42 structure engages with the rack 47. As gear 42 moves upward, the structure rotates, causing the sliding column 43 to rotate vertically upward. Upon rotation 180°, gear 42 separates from the rack 47, and the sliding column 43 engages with the upper limiting bracket 46. Continuing to move gear 42 upward causes the sliding column 43 to slide within the upper limiting bracket 46 until it reaches one end. At this point, gear 42 has moved to its maximum distance, which is also the point where the cam 32's maximum diameter contacts the abutment rod 37. As the rotating block 22 moves and rotates with gear 42, the screw 44 abuts against the upper abutment plate 12, causing the piston structure inside the injection tube 10 to move.This allows the culture medium to be injected into the culture dish below the dispensing syringe 7, thus forming a culture medium. When the flipping block 22 flips until the hook 23 engages with the contact plate 12, it drives the pull rod 11 downward, allowing the culture medium to be drawn from the outer connecting tube 9 into the injection tube 10. The dispensing syringe 7 has a common single-pass structure, meaning the culture medium can only flow out of the dispensing syringe 7, preventing air from being drawn in. By controlling the length of the screw 44 screwed into the flipping block 22, the distance the screw 44 pushes the contact plate 12 upward can be changed. That is, while keeping the screw 44 structure unchanged, each flipping of the flipping block 22 ensures that the amount of culture medium entering the culture dish is the same, thus ensuring the consistency of the culture medium composition. The operation of adding culture medium to the culture dish is simple, ensuring high culture medium dispensing efficiency. The operator only needs to turn the crank handle 17 to complete the operation.

[0028] The above description of the embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A cell culture medium storage and dispensing device, comprising a base (1), characterized in that, The upper end of the base (1) is fixedly connected to a support column (2), the upper end of the support column (2) is fixedly connected to a limit rail (26), the limit rail (26) is connected to a connecting frame (5), and the connecting frame (5) is connected to a fixing sleeve (6). The limiting rail (26) is connected to a dispensing tray (3), the dispensing tray (3) is provided with a placement groove (4), the lower end of the dispensing tray (3) is fixedly connected to a rotating rod (27), the lower end of the rotating rod (27) is connected to a rotating umbrella (24), the upper end of the base (1) is connected to a center seat (25), and the bottom end of the rotating umbrella (24) is rotatably connected to the center seat (25); The fixed sleeve (6) is connected to a dispensing needle tube (7), the dispensing needle tube (7) is connected to a connecting tube (8), the connecting tube (8) is connected to an outer tube tube (9) and an injection tube (10), the injection tube (10) is connected to a pull rod (11), and one end of the pull rod (11) is fixedly connected to an abutment piece (12). A flip block (22) is provided below the abutment piece (12). One end of the flip block (22) is threaded with a screw (44), and the other end of the flip block (22) is fixedly connected with a hook (23). A sliding column (43) is fixedly connected to the flip block (22). A gear (42) is fixedly connected to the outside of the flip block (22). A flip rod (29) is fixedly connected to one side of the gear (42). A limit post (20) is fixedly connected to the upper end of the base (1). A lifting rod (45) is slidably connected to the limit post (20). A lifting block (41) is fixedly connected to the upper end of the lifting rod (45). The flip rod (29) is rotatably connected to the lifting block (41). A transmission mechanism for driving the lifting block (41) to rise and fall is provided at the upper end of the base (1).

2. The cell culture medium storage and dispensing device according to claim 1, characterized in that, The transmission mechanism includes a deflection frame (35), with rotating tubes (36) connected to both ends of the deflection frame (35). A contact rod (37) is connected to the rotating tube (36), and a transmission rod (38) is connected to the outside of another rotating tube (36). A hinge frame one (15) and a hinge frame three (30) are fixedly connected to the upper end of the base (1). A driving member is provided on one side of the contact rod (37) to drive the contact rod (37) to deflect. A toggle member is connected to the transmission rod (38) to move the flipping rod (29).

3. The cell culture medium storage and dispensing device according to claim 2, characterized in that, The driving component includes a crank handle (17), and a rotating seat one (13) and a rotating seat two (14) are fixedly connected to the upper end of the base (1). One end of the crank handle (17) is connected to a limit ring (28) and a cam (32).

4. A cell culture medium storage and dispensing device according to claim 3, characterized in that, One end of the crank handle (17) is connected to a semi-cylinder (33), and a toggle post (34) is connected to the semi-cylinder (33). The semi-cylinder (33) and the toggle post (34) are connected to the rotating umbrella (24).

5. A cell culture medium storage and dispensing device according to claim 2, characterized in that, The actuating component includes a lifting rod (39), and a hinge frame two (16) is fixedly connected to the upper end of the base (1). One end of the lifting rod (39) is rotatably connected to the hinge frame two (16). The lifting rod (39) is connected to the transmission rod (38) through a connecting buckle (19). One end of the lifting rod (39) is provided with a sliding groove (40), and one end of the flipping rod (29) is slidably connected to the sliding groove (40).

6. The cell culture medium storage and dispensing device according to claim 1, characterized in that, The upper end of the lifting block (41) is fixedly connected to a support suction cup (18).

7. A cell culture medium storage and dispensing device according to claim 1, characterized in that, A connecting block (21) is fixedly connected to the upper end of the base (1), a fixing frame (31) is connected to the connecting block (21), and a rack (47) is connected to the fixing frame (31).

8. A cell culture medium storage and dispensing device according to claim 7, characterized in that, The fixed frame (31) is connected to the upper and lower ends of the limiting frame (46), the limiting frame (46) and the sliding column (43) cooperate with each other, and the rack (47) and the gear (42) cooperate with each other.

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