Aerobic treatment biological synergistic method

By using dynamic monitoring and feedback control, the problem of low activity of compound microbial agents in traditional aerobic treatment has been solved, achieving efficient expansion and rapid fermentation of compound microbial communities, thereby improving wastewater treatment efficiency and effectiveness.

CN121759340APending Publication Date: 2026-03-31SHENZHEN FENGYUN ECOLOGICAL ENERGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-31
Publication Date
2026-03-31

AI Technical Summary

Technical Problem

In traditional aerobic treatment methods, compound microbial agents are not acclimatized to specific water qualities beforehand, resulting in low activity and unstable degradation efficiency in the target wastewater system. The fermentation process lacks dynamic control, which affects the efficacy of the microbial agents.

Method used

Through dynamic monitoring and feedback control, the aerobic fermentation process is precisely regulated. Based on the sludge carrying capacity characteristic value and oxygen consumption curve, the input of compound bacteria and environmental parameters are adjusted in real time to ensure that the bacterial community is in the highest activity state and avoid nutrient deficiency or inhibition by metabolic products.

Benefits of technology

It achieves efficient and rapid expansion of the complex microbial community, and the precise matching of microbial agent dosage with raw material load improves fermentation efficiency and microbial activity, shortens fermentation time, and enhances wastewater treatment effect.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention relates to the technical field of aerobic treatment biology, in particular to an aerobic treatment biological synergy method which comprises the following steps: carrying out preliminary sedimentation treatment on sewage or organic waste to be treated to obtain a culture medium raw material, introducing the culture medium raw material into a culture tank, and determining a sludge bearing capacity characteristic value; determining the input amount of compound bacteria based on the characteristic value of the sludge bearing capacity and carrying out aerobic fermentation; in the aerobic fermentation process of the biological bacteria, determining the fermentation rate based on the oxygen consumption in the culture tank to obtain a fermentation rate change curve, and determining that the fermentation process enters an attenuation period based on the fermentation rate change curve; detecting the concentration of the compound bacteria, determining to increase the fermentation temperature and ventilation quantity based on the concentration of the compound bacteria, and continuing fermentation; and carrying out solid-liquid separation on the fermented composite flora mixed solution in the culture tank to obtain a high-concentration bacterial solution, and putting the high-concentration bacterial solution into a target sewage treatment system. According to the invention, the problems of low fermentation efficiency and insufficient activity of the composite flora in the prior art are solved.
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Description

Technical Field

[0001] This invention relates to the field of aerobic treatment biotechnology, and more particularly to a method for enhancing the efficiency of aerobic treatment. Background Technology

[0002] Aerobic biological treatment technology is widely used in the treatment of municipal sewage, industrial wastewater, and organic waste. It degrades organic matter through the metabolism of aerobic microorganisms to purify water. However, the compound microbial agents conventionally added in traditional aerobic treatment methods often lack prior acclimatization and expansion for specific water qualities, resulting in low activity and unstable degradation efficiency in the target sewage system. The fermentation process lacks a dynamic control mechanism based on real-time biological status, which can easily lead to insufficient or excessive fermentation, affecting the efficacy of the microbial agents. Furthermore, the failure to link key parameters such as sludge carrying capacity with the dosage of microbial agents results in resource waste or unsatisfactory treatment effects.

[0003] Chinese Patent Publication No. CN119191538A discloses an aerobic treatment bio-enhancing process, comprising adding wastewater, organic waste, or biogas residue / biogas slurry to a first microbial culture tank; adding a pH adjuster to adjust the pH value; and using a blower to introduce gas into the first microbial culture tank for stirring. This aerobic treatment bio-enhancing process can autonomously cultivate targeted dominant microbial communities according to different treatment objects, and by monitoring various parameters, it can accelerate the aerobic reaction process, increase the reaction degree, shorten the reaction time of aerobic treatment, and improve the process treatment effect.

[0004] However, the compound bacterial agent routinely added in this process has not undergone prior acclimatization for specific water quality, resulting in low activity during the cultivation process. Summary of the Invention

[0005] Therefore, the present invention provides an aerobic treatment method for enhancing biological efficiency, in order to overcome the problems of low fermentation efficiency and insufficient activity of compound microbial communities in the prior art.

[0006] To achieve the above objectives, the present invention provides a method for enhancing the bioefficiency of aerobic treatment, comprising: Step S1: The wastewater or organic waste to be treated is subjected to preliminary sedimentation treatment, and the amount of flocculant added is determined according to the turbidity. The separated bottom sediment is passed into the culture tank as a culture medium raw material. Step S2: Determine the sludge carrying capacity characteristic value based on the ratio of the weight of volatile suspended solids in the mixture of the culture medium raw materials to the weight of suspended solids in the mixture, so as to determine the amount of compound bacteria to be added. After adding the compound bacteria to the culture medium raw materials at the specified amount, aerobic fermentation is carried out at a preset temperature and ventilation rate. Step S3: Determine the oxygen consumption curve based on the oxygen consumption in the culture tank to determine the decay period of the fermentation process; Step S4: Detect the concentration of the compound bacteria. Based on the result that the concentration of the compound bacteria is greater than or equal to the preset concentration, determine to increase the fermentation temperature and ventilation to continue fermentation. Based on the result that the concentration of the compound bacteria is less than the preset concentration, determine to complete fermentation. Step S5: The fermented compound microbial mixture in the culture tank is subjected to solid-liquid separation to obtain a high-concentration bacterial solution, which is then introduced into the target wastewater treatment system.

[0007] Furthermore, the preliminary settlement treatment process in step S1 includes: Step S11: The wastewater or organic waste to be treated is introduced into a gravity sedimentation tank for sedimentation. Step S12: During the sedimentation process, obtain the turbidity change curve based on the turbidity of the supernatant, and predict the sedimentation completion time based on the turbidity change rate. Step S13: Based on the result that the settling completion time is greater than or equal to the predetermined completion time, determine the amount of flocculant to be added; or based on the result that the settling completion time is less than the predetermined completion time, determine to continue settling until the turbidity change rate is less than 5%. Step S14: Filter and separate the bottom precipitate to obtain the culture medium raw material; The predetermined completion time is 1 hour, and the amount of flocculant added is the product of the standard amount of flocculant added and the turbidity change rate.

[0008] Furthermore, step S1 also includes adjusting the pH of the culture medium raw material to 7-8.5 using a pH adjuster.

[0009] Further, in step S2, the process of determining the characteristic value of sludge carrying capacity includes: Step S21: Take 100g of culture medium raw material sample, filter it, dry it at 100℃ to obtain suspended solids, and weigh it to determine the weight of the suspended solids in the mixture. Step S22: The suspended solids are calcined at 600°C for 60 minutes, and the weight difference before and after calcination is determined as the weight of the volatile suspended solids in the mixture. Step S23: The ratio of the weight of volatile suspended solids in the mixture to the weight of suspended solids in the mixture is determined as the characteristic value of sludge carrying capacity.

[0010] Furthermore, the process of determining the amount of compound bacteria to be added based on the sludge carrying capacity characteristic value includes: Step S24: The ratio of the sludge bearing capacity characteristic value to the preset sludge bearing capacity characteristic value is determined as the bearing capacity difference value; Step S25: Compare and analyze the bearing capacity difference value with the bearing capacity difference threshold. Step S26: Based on the result that the bearing capacity difference value is greater than or equal to the bearing capacity difference threshold, determine to increase the preset compound bacteria input amount, or based on the result that the bearing capacity difference value is less than the bearing capacity difference threshold, determine to feed with the preset compound bacteria input amount. The preset amount of compound bacteria is 3‰ of the total weight of the culture medium raw materials, and the increase in the amount of compound bacteria is the product of the difference between the carrying capacity difference value and the carrying capacity difference threshold and the preset amount of compound bacteria.

[0011] Further, in step S3, the process of determining the decay period includes: Step S31: Obtain the oxygen consumption of the culture tank per unit time and plot the oxygen consumption curve. Step S32: Based on the decreasing trend of the oxygen consumption curve, it is determined that the fermentation process has entered the decay period.

[0012] Furthermore, step S4 also includes: Step S41: Determine the concentration difference value based on the difference between the concentration of the compound bacteria and the preset concentration; Step S42: Compare and analyze the concentration difference value with a preset concentration difference threshold; Step S43: Based on the result that the concentration of the compound bacteria is less than the preset concentration, determine to increase the fermentation temperature and ventilation and continue fermentation, and based on the result that the concentration of the compound bacteria is greater than or equal to the preset concentration, determine to complete fermentation.

[0013] Furthermore, in step S43, the fermentation temperature is increased by 3°C from the original preset temperature, and the ventilation volume is increased by 10% from the original preset ventilation volume. After the adjustment is completed, steps S41 to S43 are repeated.

[0014] Furthermore, in step S5, the dosage of the high-concentration bacterial solution is 0.5‰ of the total amount of pollutants treated daily by the target wastewater system.

[0015] Furthermore, the compound bacteria include 5 parts aerobic degrading bacteria, 3 parts Rhodococcus strains, 3 parts Bacillus subtilis, and 2 parts Pseudomonas strains.

[0016] Compared with the prior art, the beneficial effects of the present invention are that, through dynamic monitoring and feedback control, the aerobic fermentation process is precisely regulated to achieve efficient and rapid expansion of the complex microbial community; by judging the metabolic stage of the microorganisms in real time through key parameters and applying the most suitable environmental pressure, the microbial community is always kept in the highest active growth state, avoiding the decrease in efficiency caused by insufficient nutrition or inhibition of metabolites.

[0017] Furthermore, the amount of compound microorganisms added is dynamically adjusted based on the sludge carrying capacity characteristics, ensuring precise matching between the microbial agent dosage and the raw material load. This avoids waste of microbial agents while maximizing microbial activity during fermentation. When the organic matter content of the culture medium is high, it means that there are sufficient nutrients available for microbial growth and a strong ecosystem carrying capacity. In this case, adding a larger initial amount of microorganisms can quickly utilize the abundant nutrients, rapidly enter the exponential growth phase, seize ecological niches, and shorten fermentation time. Conversely, if the nutrient base is poor, adding too many microorganisms will cause the microbial community to compete for limited resources, which will inhibit overall growth. Therefore, dynamically adjusting the amount of microorganisms added based on the nutrient base is an optimization strategy that conforms to the principles of microbial community dynamics.

[0018] Furthermore, by plotting oxygen consumption curves to identify the fermentation decay phase, we can achieve biostate-driven control of the fermentation process and improve the accuracy of determining the fermentation endpoint. During the logarithmic growth and stationary phases of fermentation, the microbial community is abundant and metabolism is vigorous, maintaining a high oxygen consumption rate. When easily degradable organic matter in the culture medium is depleted, or metabolic waste accumulates to a certain level, the microbial community enters the decay phase, the metabolic rate slows down, and the oxygen consumption rate decreases significantly. Therefore, identifying the start of the decay phase through the inflection point of the oxygen consumption rate curve has strong physiological and biochemical basis and is more accurate than simply relying on time.

[0019] Furthermore, during the decay period, the decision to continue fermentation is based on the bacterial concentration, and environmental parameters are adjusted accordingly to achieve intelligent regulation guided by the needs of the microbial community. This ensures the acquisition of a highly active, high-concentration compound bacterial solution. When the system enters the decay period but the bacterial concentration remains high, the temperature and ventilation are moderately increased to apply a mild environmental pressure. This induces a stress response in the microorganisms, potentially activating certain dormant degradation enzyme systems, prompting the microbial community to utilize the remaining complex substrate for secondary growth. This not only increases yield but also enhances the activity of the microbial community, making it more competitive after being introduced into the target wastewater system. Attached Figure Description

[0020] Figure 1 This is a flowchart of the aerobic treatment bio-enhancing method according to an embodiment of the present invention; Figure 2 This is a flowchart of step S1 of the aerobic treatment bio-enhancing method according to an embodiment of the present invention; Figure 3 This is a flowchart of step S2 of the aerobic treatment bio-enhancing method according to an embodiment of the present invention; Figure 4 This is a flowchart of step S4 of the aerobic treatment bio-enhancing method according to an embodiment of the present invention.

[0021] Figure 5 This is a turbidity curve diagram of step S1 sedimentation process in the aerobic treatment bio-enhancing method of embodiments 1-3 of the present invention; Figure 6This is a graph showing the oxygen consumption curve in step S3 of the aerobic treatment bio-enhancing method in Examples 1-3 of the present invention. Detailed Implementation

[0022] To make the objectives and advantages of the present invention clearer, the present invention will be further described below with reference to embodiments; it should be understood that the specific embodiments described herein are merely for explaining the present invention and are not intended to limit the present invention.

[0023] Preferred embodiments of the present invention will now be described with reference to the accompanying drawings. Those skilled in the art should understand that these embodiments are merely illustrative of the technical principles of the present invention and are not intended to limit the scope of protection of the present invention.

[0024] Please see Figure 1 The following are flowcharts of the aerobic treatment bio-enhancing method according to embodiments of the present invention; The aerobic treatment bio-enhancing method of this invention includes: Step S1: The wastewater or organic waste to be treated is subjected to preliminary sedimentation treatment, and the amount of flocculant added is determined according to the turbidity. The separated bottom sediment is passed into the culture tank as a culture medium raw material. The organic waste is any one of industrial sludge, crop straw, livestock and poultry manure, and kitchen waste; Step S2: Determine the sludge carrying capacity characteristic value based on the ratio of the weight of volatile suspended solids in the mixture of the culture medium raw materials to the weight of suspended solids in the mixture, so as to determine the amount of compound bacteria to be added. After adding the compound bacteria to the culture medium raw materials at the specified amount, aerobic fermentation is carried out at a preset temperature and ventilation rate. Step S3: Determine the oxygen consumption curve based on the oxygen consumption in the culture tank to determine the decay period of the fermentation process; Step S4: Detect the concentration of the compound bacteria. Based on the result that the concentration of the compound bacteria is greater than or equal to the preset concentration, determine to increase the fermentation temperature and ventilation to continue fermentation. Based on the result that the concentration of the compound bacteria is less than the preset concentration, determine to complete fermentation. Step S5: The fermented compound microbial mixture in the culture tank is subjected to solid-liquid separation to obtain a high-concentration bacterial solution, which is then introduced into the target wastewater treatment system.

[0025] Please see Figure 2 The diagram shows a flowchart of step S1 of the aerobic treatment bio-enhancing method according to an embodiment of the present invention. In step S1, the preliminary sedimentation treatment process includes: Step S11: The wastewater or organic waste to be treated is introduced into a gravity sedimentation tank for sedimentation. Step S12: During the sedimentation process, obtain the turbidity change curve based on the turbidity of the supernatant, and predict the sedimentation completion time based on the turbidity change rate. The turbidity change rate is continuously monitored by an online turbidity meter, and the change rate is calculated as the ratio of the difference between the current turbidity and the turbidity 30 minutes ago to the time interval.

[0026] Step S13: Based on the result that the settling completion time is greater than or equal to the predetermined completion time, determine the amount of flocculant to be added; or based on the result that the settling completion time is less than the predetermined completion time, determine to continue settling until the turbidity change rate is less than 5%. Step S14: Filter and separate the bottom precipitate to obtain the culture medium raw material; The predetermined completion time is 1 hour, and the amount of flocculant added is the product of the standard amount of flocculant added and the turbidity change rate.

[0027] It is understood that in this embodiment, the standard addition amount of the flocculant is 1 mg / L, and the ratio can be adjusted according to water quality fluctuations. Preferably, the flocculant is polyaluminum chloride. The sedimentation tank is a rectangular horizontal flow sedimentation tank with a hydraulic retention time of not less than 2 hours. The culture tank is a fermentation tank with a stirring and aeration device, with an effective volume of 1m³ and a stirring speed of 50~200rpm. The solid-liquid separation operation is carried out using a plate and frame filter press or a centrifuge.

[0028] Gravity sedimentation is used to separate solids and liquids. Flocculants are used to promote the aggregation of fine particles through charge neutralization and bridging, which accelerates sedimentation, reduces the load of suspended solids, and avoids blockage or interference in the subsequent fermentation process. At the same time, the precipitate concentrates organic matter, providing an inexpensive substrate for cultivating microorganisms. The precipitate also serves as a raw material for culture medium, providing organic carbon sources and nutrients, thus realizing the resource utilization of waste.

[0029] Specifically, step S1 further includes adjusting the pH of the culture medium raw material to 7-8.5 using a pH adjuster.

[0030] Please see Figure 3 The diagram shows a flowchart of step S2 in the aerobic treatment bio-enhancing method according to an embodiment of the present invention. In step S2, the process of determining the sludge carrying capacity characteristic value includes: Step S21: Take 100g of culture medium raw material sample, filter it, dry it at 100℃ to obtain suspended solids, and weigh it to determine the weight of the suspended solids in the mixture. Step S22: The suspended solids are calcined at 600°C for 60 minutes, and the weight difference before and after calcination is determined as the weight of the volatile suspended solids in the mixture. Step S23: The ratio of the weight of volatile suspended solids in the mixture to the weight of suspended solids in the mixture is determined as the characteristic value of sludge carrying capacity.

[0031] Specifically, the process of determining the amount of compound bacteria to be added based on the sludge carrying capacity characteristic value includes: Step S24: The ratio of the sludge bearing capacity characteristic value to the preset sludge bearing capacity characteristic value is determined as the bearing capacity difference value, where the preset sludge bearing capacity characteristic value is 0.75; Step S25: Compare and analyze the bearing capacity difference value with the bearing capacity difference threshold, where the bearing capacity difference threshold is 0.05; It is understood that in this embodiment, the preset sludge carrying capacity characteristic value is 0.75 and the carrying capacity difference threshold is 0.05. Both are statistical medians based on common municipal sewage culture medium raw materials, which are obtained through statistical experiments on typical municipal sewage samples to ensure that the amount of bacteria added matches the nutrient load.

[0032] Step S26: Based on the result that the bearing capacity difference value is greater than or equal to the bearing capacity difference threshold, determine to increase the preset compound bacteria input amount, or based on the result that the bearing capacity difference value is less than the bearing capacity difference threshold, determine to feed with the preset compound bacteria input amount. The preset amount of compound bacteria is 3‰ of the total weight of the culture medium raw materials, and the increase in the amount of compound bacteria is the product of the difference between the carrying capacity difference value and the carrying capacity difference threshold and the preset amount of compound bacteria.

[0033] The sludge carrying capacity characteristic value reflects the biodegradability of organic matter. Volatile suspended solids represent the organic matter content, and suspended solids represent the total solids. A high ratio indicates a high organic load, requiring more microorganisms to balance the substrate and microbial community ratio. The ratio is used to determine the amount of compound bacteria to be added in order to optimize degradation efficiency.

[0034] Specifically, in step S3, the process of determining the fermentation endpoint includes: Step S31: Obtain the oxygen consumption of the culture tank per unit time and plot the oxygen consumption curve. The oxygen consumption is detected in real time by a gas analyzer at the exhaust port of the culture tank. Preferably, an oxygen electrode or mass spectrometer is used for detection, with a unit time of one minute. The curve is automatically generated by a data acquisition system. Step S32: Based on the decreasing trend of the oxygen consumption curve, it is determined that the fermentation process has entered the decay period. Specifically, when the oxygen consumption curve decreases by more than 5% within 30 minutes, the fermentation process is considered to have entered the decay phase.

[0035] In aerobic fermentation, oxygen consumption is positively correlated with microbial metabolic activity. Initially, oxygen consumption is rapid, corresponding to the logarithmic growth phase; later, consumption decreases, indicating a reduction in substrate and the microorganisms entering the decay phase. The oxygen consumption curve is similar to the microbial growth curve, and the decay phase marks the approaching end of fermentation. Real-time monitoring of oxygen consumption can avoid over-fermentation that wastes energy or premature termination of fermentation, ensuring that fermentation stops at the optimal point and improving microbial activity.

[0036] Please see Figure 4 The diagram shows a flowchart of step S4 in the aerobic treatment bio-enhancing method according to an embodiment of the present invention. Step S4 further includes: Step S41: Determine the concentration difference value based on the difference between the concentration of the compound bacteria and the preset concentration; Step S42: Compare and analyze the concentration difference value with a preset concentration difference threshold, wherein the preset concentration difference threshold is 0.11; Step S43: Based on the result that the concentration of the compound bacteria is less than the preset concentration, determine to increase the fermentation temperature and ventilation and continue fermentation, and based on the result that the concentration of the compound bacteria is greater than or equal to the preset concentration, determine to complete fermentation.

[0037] The concentration of the compound bacteria was detected by plate counting or optical density method, and samples were taken and tested every 2 hours.

[0038] It is understood that in this embodiment, the preset concentration is 1.0 g / L, which was determined through preliminary experiments and is the minimum effective concentration to ensure bacterial activity and fermentation efficiency. Specifically, in step S43, the fermentation temperature is increased by 3°C from the original preset temperature, and the ventilation volume is increased by 10% from the original preset ventilation volume. After the adjustment is completed, steps S41 to S43 are repeated.

[0039] Microbial concentration directly determines fermentation efficiency. A preset concentration is used as a threshold. When the concentration is below the threshold, increasing the temperature and ventilation can stimulate microbial growth. Repeated testing ensures that the microbial community reaches its optimal state. By optimizing environmental conditions, the maximum specific growth rate of microorganisms can be maintained, the fermentation time can be shortened, and a highly active bacterial solution can be obtained.

[0040] Specifically, in step S5, the dosage of the high-concentration bacterial solution is 0.5‰ of the total amount of pollutants treated daily by the target wastewater system.

[0041] Specifically, the compound bacteria include 5 parts aerobic degrading bacteria, 3 parts Rhodococcus strains, 3 parts Bacillus subtilis, and 2 parts Pseudomonas strains. Example

[0042] Step S1: Perform preliminary sedimentation treatment on urban domestic sewage by introducing the sewage into a gravity sedimentation tank, measuring the turbidity of the supernatant, and plotting the turbidity change curve. Please refer to [link / reference]. Figure 5 As shown, this is a turbidity curve of the sedimentation process in step S1 of this embodiment of the invention. When the turbidity is greater than the preset turbidity after 1 hour of sedimentation, the initial sedimentation time is longer than the predetermined completion time. Flocculant is added at a concentration of 1 mg / L. After sedimentation, the bottom precipitate is filtered and separated to obtain the culture medium raw material. The pH of the culture medium raw material is adjusted to 7.5 using sodium carbonate pH adjuster.

[0043] Step S2: Take 100g of culture medium raw material sample, dry it at 100℃ and ignite it at 600℃, and measure the characteristic value of sludge carrying capacity to be 0.8. Calculate the compound bacteria input amount to be 3.3‰.

[0044] After adding the compound bacteria, aerobic fermentation was carried out at a preset temperature of 35°C and an aeration rate of 100L / min.

[0045] Step S3: During fermentation, monitor oxygen consumption in real time and plot the oxygen consumption curve. Please refer to [link to relevant documentation]. Figure 6 As shown, it is an oxygen consumption curve of step S3 in the embodiment of the present invention. It is found that the curve drops by more than 5% in the 5th hour, indicating that the fermentation has entered the decay period.

[0046] In step S4, the concentration of the compound bacteria was determined to be 0.8 g / L using the plate count method, while the preset concentration was 1.0 g / L. Therefore, the fermentation temperature and aeration rate were increased. The fermentation temperature was increased by 3°C to 38°C, and the aeration rate was increased by 10% to 110 L / min. After fermenting for another 12 hours, the concentration of the compound bacteria was retested and confirmed to be 1.2 g / L, indicating that fermentation was complete.

[0047] Step S5: Obtain a high-concentration bacterial solution, add it to the sewage treatment system, and test the sewage treatment effect.

[0048] Table 1 Wastewater treatment effect in Example 1 ; Example 2: In this embodiment, the wastewater to be treated is not pretreated, and cultivation begins directly from step S2, with the rest being the same as in Example 1.

[0049] Table 2 Wastewater Treatment Effect in Example 2 ; The wastewater treatment effects of Examples 1 and 2 show that preliminary sedimentation treatment, for wastewater with high suspended solids content and abnormal pH value, provides a stable and suitable initial environment for the growth and metabolism of the complex bacteria through sedimentation and pH adjustment. This is the basis for efficient fermentation and achieving top-level treatment results.

[0050] Example 3: In this embodiment, mixed wastewater from a chemical industrial park is used as the culture medium raw material, and the rest is the same as in Example 1.

[0051] Table 3 Wastewater treatment effect in Example 3 ; It can be seen that the effluent effect of Example 3 is better than that of Example 2 but slightly worse than that of Example 1, with COD of 15 mg / L and BOD5 of 10 mg / L, indicating that it still has a good treatment effect on medium suspended solids and high concentration of organic wastewater. However, compared with Example 1 after pretreatment, its ability to completely remove recalcitrant organic matter is slightly weaker. The pH of the effluent of Example 3 is stable at 7.8, proving that the alkalinity of the influent can be effectively neutralized through the biological regulation during the cultivation process.

[0052] Comparative Example 1: In this comparative example, the same compound bacterial strain as in Example 1 was used for cultivation, and the environmental parameters during the cultivation process were all preset values ​​and were not adjusted.

[0053] Comparative Example 2: In this comparative example, the same compound bacterial strain as in Example 1 was used for cultivation in the nutrient solution, and the cultivation process was the same as in Example 1.

[0054] Table 4 Comparison of cultivation effects in comparative studies ; Without dynamic adjustment in Comparative Example 1, the fermentation process becomes inefficient and the final bacterial agent quality fails to meet standards, which seriously affects the final wastewater treatment effect. This shows that the real-time monitoring and feedback adjustment mechanism in Example 1 is the core of ensuring efficient and high-quality production of the bacterial agent.

[0055] Comparative Example 2 shows that although high concentrations of bacteria can be cultivated in nutrient solutions, the cost is high, and the cultivated bacteria lack adaptability to real wastewater, resulting in poor treatment performance due to incompatibility with local conditions. The targeted acclimatization strategy for bacteria used in Example 1 is not only economically feasible but also more scientifically sound in terms of technology.

[0056] As shown in Tables 1 and 4, using the method of the present invention, the concentration of compound bacteria can reach more than 1.2 g / L, the fermentation cycle is shortened to 12 hours, the efficiency is significantly improved compared with Comparative Example 1, and the COD of the effluent is consistently below 20 mg / L, indicating that the bacterial community has high activity and the treatment effect is significant.

[0057] The technical solution of the present invention has been described above with reference to the preferred embodiments shown in the accompanying drawings. However, it will be readily understood by those skilled in the art that the scope of protection of the present invention is obviously not limited to these specific embodiments. Without departing from the principles of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will all fall within the scope of protection of the present invention.

[0058] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for enhancing the bioefficiency of aerobic treatment, characterized in that, include: Step S1: Perform preliminary sedimentation treatment on the wastewater or organic waste to be treated, and determine the amount of flocculant to be added based on the turbidity during the preliminary sedimentation treatment process. Use the separated bottom sediment as the culture medium raw material and put it into the culture tank. Step S2: Determine the sludge carrying capacity characteristic value based on the ratio of the weight of volatile suspended solids in the mixture of the culture medium raw materials to the weight of suspended solids in the mixture, so as to determine the amount of compound bacteria to be added. After adding the compound bacteria to the culture medium raw materials at the specified amount, aerobic fermentation is carried out at a preset temperature and ventilation rate. Step S3: Determine the oxygen consumption curve based on the oxygen consumption in the culture tank to determine the decay period of the fermentation process; Step S4: Detect the concentration of the compound bacteria. Based on the result that the concentration of the compound bacteria is greater than or equal to the preset concentration, determine to increase the fermentation temperature and ventilation to continue fermentation. Based on the result that the concentration of the compound bacteria is less than the preset concentration, determine to complete fermentation. Step S5: The fermented compound microbial mixture in the culture tank is subjected to solid-liquid separation to obtain a high-concentration bacterial solution, which is then introduced into the target wastewater treatment system.

2. The aerobic treatment bio-enhancing method according to claim 1, characterized in that, The preliminary settlement treatment process in step S1 includes: Step S11: The wastewater or organic waste to be treated is introduced into a gravity sedimentation tank for sedimentation. Step S12: During the sedimentation process, obtain the turbidity change curve based on the turbidity of the supernatant, and predict the sedimentation completion time based on the turbidity change rate. Step S13: Based on the result that the settling completion time is greater than or equal to the predetermined completion time, determine the amount of flocculant to be added; or based on the result that the settling completion time is less than the predetermined completion time, determine to continue settling until the turbidity change rate is less than 5%. Step S14: Filter and separate the bottom precipitate to obtain the culture medium raw material; The predetermined completion time is 1 hour, and the amount of flocculant added is the product of the standard amount of flocculant added and the turbidity change rate.

3. The aerobic treatment bio-enhancing method according to claim 2, characterized in that, Step S1 further includes using a pH adjuster to adjust the pH of the culture medium raw material to 7-8.

5.

4. The aerobic treatment bio-enhancing method according to claim 3, characterized in that, In step S2, the process of determining the characteristic value of sludge carrying capacity includes: Step S21: Take 100g of culture medium raw material sample, filter it, dry it at 100℃ to obtain suspended solids, and weigh it to determine the weight of the suspended solids in the mixture. Step S22: The suspended solids are calcined at 600°C for 60 minutes, and the weight difference before and after calcination is determined as the weight of the volatile suspended solids in the mixture. Step S23: The ratio of the weight of volatile suspended solids in the mixture to the weight of suspended solids in the mixture is determined as the characteristic value of sludge carrying capacity.

5. The aerobic treatment bio-enhancing method according to claim 4, characterized in that, The process of determining the amount of compound bacteria input based on the sludge carrying capacity characteristic value includes: Step S24: The ratio of the sludge bearing capacity characteristic value to the preset sludge bearing capacity characteristic value is determined as the bearing capacity difference value; Step S25: Compare and analyze the bearing capacity difference value with the bearing capacity difference threshold. Step S26: Based on the result that the bearing capacity difference value is greater than or equal to the bearing capacity difference threshold, determine to increase the preset compound bacteria input amount, or based on the result that the bearing capacity difference value is less than the bearing capacity difference threshold, determine to feed with the preset compound bacteria input amount. The preset amount of compound bacteria is 3‰ of the total weight of the culture medium raw materials, and the increase in the amount of compound bacteria is the product of the difference between the carrying capacity difference value and the carrying capacity difference threshold and the preset amount of compound bacteria.

6. The aerobic treatment bio-enhancing method according to claim 5, characterized in that, In step S3, the process of determining the decay period includes: Step S31: Obtain the oxygen consumption of the culture tank per unit time and plot the oxygen consumption curve. Step S32: Based on the decreasing trend of the oxygen consumption curve, it is determined that the fermentation process has entered the decay period.

7. The aerobic treatment bio-enhancing method according to claim 6, characterized in that, Step S4 further includes: Step S41: Determine the concentration difference value based on the difference between the concentration of the compound bacteria and the preset concentration; Step S42: Compare and analyze the concentration difference value with a preset concentration difference threshold; Step S43: Based on the result that the concentration of the compound bacteria is less than the preset concentration, determine to increase the fermentation temperature and ventilation and continue fermentation, and based on the result that the concentration of the compound bacteria is greater than or equal to the preset concentration, determine to complete fermentation.

8. The aerobic treatment bio-enhancing method according to claim 7, characterized in that, In step S43, the fermentation temperature is increased by 3°C from the original preset temperature, and the ventilation volume is increased by 10% from the original preset ventilation volume. After the adjustment is completed, steps S41 to S43 are repeated.

9. The aerobic treatment bio-enhancing method according to claim 8, characterized in that, In step S5, the dosage of the high-concentration bacterial solution is 0.5‰ of the total amount of pollutants treated daily by the target wastewater system.

10. The aerobic treatment bio-enhancing method according to claim 9, characterized in that, The compound bacteria consist of 5 aerobic degrading bacteria, 3 Rhodococcus strains, 3 Bacillus subtilis strains, and 2 Pseudomonas strains.

Citation Information

Patent Citations

  • Aerobic treatment biological synergistic process

    CN119191538A