Method for rapidly detecting valence state of antimony ions and application

Abstract

The invention provides a method for rapidly detecting antimony ions and application, the method for rapidly detecting antimony ions comprises the following steps: dividing a solution to be detected into two parts, adding a fluorescent probe TPE-4TA into the first part of the solution to be detected, and uniformly mixing; adding a fluorescent probe PBET into the second solution to be detected, and uniformly mixing; under excitation of light with the wavelength of 380 nm, the fluorescence intensity I460 of the first solution at the position of 460 nm and the fluorescence intensity I500 of the second solution at the position of 500 nm are measured respectively; judging the valence state of antimony ions according to the fluorescence signal; and respectively substituting the measured I460 and I500 into a pre-established Sb < 3 + > and Sb < 5 + > standard curve, and calculating to obtain the concentrations of Sb < 3 + > and Sb < 5 + > in the solution to be detected. The method has the advantages of simplicity and convenience in operation, quick reaction and high selectivity, and can realize quick detection of antimony ions with different valence states so as to meet actual environmental sample monitoring requirements.

Description

Technical Field

[0001] This invention relates to metal ion detection technology, and more particularly to a method and application for rapid detection of antimony ions. Background Technology

[0002] Antimony (Sb) is a common heavy metal pollutant found in the environment, widely originating from wastewater from mining, smelting, chemical, and electronics industries. Antimony in the environment is primarily present as Sb. 3+ With Sb 5+ Two valence states exist, where Sb 3+ Its toxicity and migration were significantly higher than those of Sb. 5+ Accurately distinguish and quickly detect Sb 3+ With Sb 5+ This is of great significance for environmental risk assessment and ecological restoration. Existing detection methods such as atomic absorption spectrometry (AAS), inductively coupled plasma mass spectrometry (ICP-MS), and high-performance liquid chromatography (HPLC) offer high sensitivity, but are complex, time-consuming, and costly, making them unsuitable for rapid on-site detection. Some existing fluorescent probe methods primarily target total antimony, lacking valence state specificity. Therefore, there is an urgent need for a method to detect antimony ions with different valence states (Sb). 3+ With Sb 5+ Rapid detection methods are needed to meet the practical needs of environmental sample monitoring. Summary of the Invention

[0003] The purpose of this invention is to address the problems of conventional detection methods being complex, costly, and unable to rapidly detect antimony ions on-site, by proposing a rapid detection method for antimony ions (Sb). 3+ With Sb 5+ This method has the advantages of simple operation, rapid reaction, and high selectivity, and can realize the reaction of antimony ions (Sb) in different valence states. 3+ With Sb 5+ Rapid detection is available to meet the needs of real-world environmental sample monitoring.

[0004] It should be noted that, in this invention, unless otherwise specified, the specific meaning of "comprising" in relation to composition and description includes both open-ended meanings such as "comprising," "including," etc., and closed-ended meanings such as "composed of," "consisting of," etc., and similar meanings.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is: a method for rapid detection of antimony ions, comprising the following steps:

[0006] Step 1: Divide the test solution into two portions. Add the fluorescent probe TPE-4TA to the first portion of the test solution and mix well. Add the fluorescent probe PBET to the second portion of the test solution and mix well.

[0007] Step 2: Under 380nm wavelength light excitation, measure the fluorescence intensity I of the first solution at 460nm. 460 The fluorescence intensity I of the second solution at 500 nm 500 ;

[0008] Step 3: Determine whether the test solution contains antimony ions and their valence state (Sb) based on the fluorescence signal. 3+ Sb 5+ When neither of the two test solutions shows fluorescence enhancement, it indicates that the test solution does not contain Sb. 3+ and Sb 5+ If the first test solution shows enhanced fluorescence, it indicates that the test solution contains Sb. 3+ If the second test solution shows enhanced fluorescence, it indicates that the test solution contains Sb. 5 + If both test solutions show increased fluorescence, it indicates that the test solutions simultaneously contain Sb. 3+ and Sb 5+ .

[0009] Furthermore, the fluorescent probe PBET is the PBET described in Bioorganic & Medicinal Chemistry (June 22, 2021, version 1), entitled "A Fluorogenic Labeling of Live Viruses".

[0010] Furthermore, the fluorescent probe TPE-4TA was the same as described in ACS Applied Materials & Interfaces (2019, Vol. 11, pp. 29619–29629), entitled "Specific and Quantitative Detection of AlbμMinin Biological Fluids by Tetrazolate-Functionalized Water-Soluble AIEgens".

[0011] Furthermore, prior to step 1, Sb is established respectively. 3+ and Sb 5+ The standard curve is the standard curve of the concentration and fluorescence intensity of the fluorescent probes TPE-4TA and PBET.

[0012] Furthermore, after step 3, the measured I 460 and I 500 Substitute Sb respectively 3+ and Sb 5+ The standard curve was used to calculate the Sb content in the test solution.3+ and Sb 5+ The concentration.

[0013] Furthermore, the fluorescent probe TPE-4TA can detect Sb. 3+ Excitation at 380 nm, blue fluorescence at 460 nm. Step 1: The content of the fluorescent probe TPE-4TA in the first test solution is 0.01-0.5 μM, preferably 0.10 μM. For example, in the detection system, take 2.0 mL of water sample solution by volume, add 20 μL of 10 μM TPE-4TA probe working solution (aqueous solution), and mix evenly to make the final concentration of the probe in the system approximately 0.10 μM.

[0014] Furthermore, the fluorescent probe PBET can detect Sb. 5+ Excitation at 380 nm, producing yellow fluorescence at 500 nm. In step 1, the concentration of the fluorescent probe PBET in the second test solution is 0.01-0.5 μM, preferably 0.10 μM. For example, in the detection system, 2.0 mL of water sample solution is taken by volume, and 20 μL of a 10 μM PBET probe working solution is added. After uniform mixing, the final concentration of the probe in the system is approximately 0.10 μM.

[0015] Furthermore, the fluorescent probe TPE-4TA is effective against Sb. 3+ The detection linear range is 0–10 mg / L, excluding 0, and the correlation coefficient (R) of the standard curve is given. 2 )≥0.99.

[0016] Furthermore, the fluorescent probe PBET is effective against Sb. 5+ The detection linear range is 0–10 mg / L, excluding 0, and the correlation coefficient (R) of the standard curve is given. 2 )≥0.99.

[0017] Further, in step 1, the fluorescent probe TPE-4TA is added to the first test solution and mixed for 5 minutes or more (preferably 5-10 minutes) to ensure the stability of the detection signal, because the fluorescent probe TPE-4TA reacts with Sb. 3+ The fluorescence signal stabilized within 5 minutes, and after stabilization, the detection signal remained stable with fluctuations of less than 5% for 10 minutes.

[0018] Further, in step 1, the fluorescent probe PBET is added to the second test solution and mixed for 4 minutes or more (preferably 4-10 minutes) to ensure the stability of the detection signal. This is because the fluorescent probe PBET reacts with Sb... 5+ The fluorescence signal stabilized within 4 minutes, and after stabilization, the detection signal remained stable with fluctuations of less than 5% for 10 minutes.

[0019] Furthermore, the pH of the test solution is 6–8, since the optimal pH range for the detection method is 6.0 to 8.0.

[0020] Furthermore, step 2 involves measuring using a fluorescence spectrometer or a fluorescence detection device equipped with appropriate filters.

[0021] Another objective of this invention is to disclose the application of a rapid method for detecting antimony ions in the field of antimony ion detection.

[0022] Furthermore, the method for rapid detection of antimony ions is particularly suitable for the detection of antimony ions in drinking water, surface water, groundwater, and soil leachates.

[0023] This invention enables rapid detection of antimony ions (Sb). 3+ With Sb 5+ This method has the following advantages compared to existing technologies:

[0024] 1) The rapid detection method for antimony ions in this invention employs two fluorescent probes (TPE-4TA and PBET), wherein the fluorescent probe TPE-4TA can detect Sb 3+ Specific recognition; under 380nm wavelength light excitation, it produces 460nm blue fluorescence, with fluorescence intensity in Sb 3+ The concentration shows a linear relationship between 0 and 10 mg / L; the fluorescent probe PBET can target Sb. 5+ Specific recognition, producing 500nm yellow fluorescence under the same excitation conditions, with fluorescence intensity in Sb 5+ The concentration shows a linear relationship from 0 to 10 mg / L. This invention employs two fluorescent probes, TPE-4TA and PBET, to form a two-dimensional probe array, achieving targeting of different valence states (Sb). 3+ With Sb 5+ This invention utilizes the specific recognition capabilities of two probes, TPE-4TA and PBET, to achieve the detection and rapid differentiation of Sb. Double negative results indicate the absence of antimony ions, double positive results indicate the presence of both antimony ions in their respective valence states, and single positive results indicate the presence of only one valence state. 3+ and Sb 5+ The precise distinction.

[0025] 2) The method for rapid detection of antimony ions in this invention has a short detection time of 4-5 minutes, making it fast and efficient;

[0026] 3) The rapid detection method for antimony ions in this invention is applicable to water bodies with a pH of 6–8 and is suitable for natural water bodies;

[0027] 4) The rapid detection method for antimony ions in this invention has strong anti-interference ability, even when Pb concentration is not less than 10 times that of antimony ions. 2+ Cd 2+ Hg 2+ Cu2+ Zn 2+ Fe 3+ Al 3+ As 5+ In the presence of single ions, the relative response and signal deviation were calculated by measuring changes in fluorescence intensity to assess the interference effect. The results showed that the fluorescence signal deviation caused by each interfering ion was less than ±30%, i.e., for Sb... 3+ or Sb 5+ Fluorescence intensity interference (change) does not exceed 30%, ensuring the reliability of detection results in samples from complex environments;

[0028] 5) The rapid detection method for antimony ions in this invention exhibits good reproducibility, stable fluorescence signal, and stable results at a concentration of 5 μg / L Sb. 3+ With 5 μg / L Sb 5+ Under sample solution conditions, five parallel detections were performed, and the relative standard deviation (RSD) of the fluorescence intensity was less than 30%;

[0029] 6) The method for rapid detection of antimony ions in this invention has a wide range of applications and is suitable for monitoring water and soil environments.

[0030] In summary, the rapid detection method for antimony ions of this invention has the advantages of high speed, high selectivity, and good repeatability, and can effectively distinguish Sb. 3+ With Sb 5+ It has good application prospects and large-scale promotion potential in the field of antimony ion detection. Attached Figure Description

[0031] Figure 1 For Sb 3+ Standard curve (concentration – fluorescence intensity at 460 nm), where the left graph represents Sb. 3+ Fluorescence standard curves in the range of 0.5–10 μg / L, the right figure shows Sb 3+ High concentration standard curves in the range of 1–10 mg / L;

[0032] Figure 2 For Sb 5+ Standard curve (concentration – fluorescence intensity at 500 nm), where the left graph represents Sb. 5+ Fluorescence standard curves in the range of 0.5–10 μg / L, the right figure shows Sb 5+ High concentration standard curves in the range of 1–10 mg / L;

[0033] Figure 3 pH for Sb 3+ The impact of the test;

[0034] Figure 4 pH for Sb 5+ The impact of the test;

[0035] Figure 5 Results for metal ion selectivity of TPE-4TA;

[0036] Figure 6 This is the result of PBET metal ion selectivity;

[0037] Figure 7 Results of TPE-4TA anti-interference experiment (coexistence of 10 times interfering ions);

[0038] Figure 8 Results of PBET anti-interference experiment (coexistence of 10 times interfering ions);

[0039] Figure 9 For Sb 3+ Results of repeatability experiments (RSD plot);

[0040] Figure 10 For Sb 5+ Results of repeatability experiments (RSD plot). Detailed Implementation

[0041] The present invention will be further described below with reference to embodiments. The description of the technical features described below is based on representative embodiments and specific examples of the present invention, but the present invention is not limited to these embodiments and specific examples. It should be noted that:

[0042] Unless otherwise stated, all units used in this specification are international standard units, and all numerical values ​​and ranges appearing in this invention should be understood to include systematic errors that are unavoidable in industrial production.

[0043] In this specification, the range of values ​​referred to as "value A to value B" refers to the range including the endpoint values ​​A and B.

[0044] In this specification, the numerical range indicated by "above" or "below" refers to the numerical range that includes the stated number.

[0045] In this specification, the word "may" has two meanings: to perform a certain process and not to perform a certain process.

[0046] In this specification, the terms "optional" or "optional" are used to indicate the use or omission of certain substances, components, procedures, application conditions, etc.

[0047] In this instruction manual, when "room temperature" or "room temperature" is used, the temperature can be 15-25℃.

[0048] Unless otherwise specified, all reagents or instruments used in this instruction manual are commercially available products.

[0049] Figure 1 For Sb 3+ Standard curve (concentration – fluorescence intensity at 460 nm), where the left graph represents Sb. 3+ Fluorescence standard curves in the range of 0.5–10 μg / L, the right figure shows Sb 3+ High concentration standard curves in the range of 1–10 mg / L; Figure 1 Sb was displayed 3+ Relationship between fluorescence response and standard curve at different concentrations (excitation wavelength 380 nm, emission peak 460 nm). The left figure shows the Sb concentration in the range of 0.5–10 ppb. 3+ The fluorescence standard curve showed a good linear relationship between fluorescence intensity and concentration (R0). 2 >0.99), can be used for trace amounts of Sb 3+ Quantitative analysis; the right figure shows the standard curve for the high concentration range of 1-10 ppm. Within this range, a slight nonlinear change in the fluorescence signal indicates that the probe may undergo partial quenching or response saturation at high concentrations. Overall results demonstrate that the TPE-4TA probe is effective against Sb. 3+ It features high sensitivity and a wide linear detection range, capable of covering trace to high concentrations of Sb in environmental samples. 3+ The measurement requirements.

[0050] Figure 2 For Sb 5+ Standard curve (concentration – fluorescence intensity at 500 nm), where the left graph represents Sb. 5+ Fluorescence standard curves in the range of 0.5–10 μg / L, the right figure shows Sb 5+ High concentration standard curves in the range of 1–10 mg / L;

[0051] The time kinetics, pH effect, single ion selectivity, metal ion interference resistance, and repeatability tests of the rapid antimony ion detection method of this invention are as follows:

[0052] Time dynamics (Sb 3+ )

[0053] 0, 1, and 5 μg / L Sb 3+ The solution was mixed with the probe TPE-4TA (10 μM), and the fluorescence intensity at 460 nm was continuously recorded over time under 380 nm excitation. The fluorescence signal stabilized within 5 minutes, and the signal fluctuation remained less than 5% within 10 minutes.

[0054] Time dynamics (Sb 5+ )

[0055] 0, 1, and 5 μg / L Sb 5+The solution was mixed with the PBET probe (10 μM), and the fluorescence intensity at 500 nm was continuously recorded over time under 380 nm excitation. The fluorescence signal stabilized within 4 minutes, and the signal fluctuation remained less than 5% within 10 minutes.

[0056] pH conditions for Sb 3+ Impact of detection

[0057] 5 μg / L Sb 3+ The solution was mixed with the TPE-4TA probe, and the fluorescence intensity was measured under different pH conditions. The results are as follows: Figure 3 As shown, the signal is strongest and most stable in the pH range of 6–8, while the signal is significantly reduced at pH 4 and pH 10.

[0058] pH conditions for Sb 5+ Impact of detection

[0059] 5 μg / L Sb 5+ The solution was mixed with the PBET probe, and the fluorescence intensity was measured under different pH conditions. The results are as follows: Figure 4 As shown, the optimal detection range is also pH 6–8, and the signal is stable and reliable.

[0060] Single ion selectivity experiment

[0061] Prepare 50 μg / L Pb 2+ Cd 2+ Hg 2+ Cu 2+ Zn 2+ Fe 3+ Al 3+ And As 5+ Metal solutions were reacted with TPE-4TA or PBET, respectively, and excited at 380 nm. The fluorescence intensities at 460 nm and 500 nm were recorded. The results are as follows: Figure 5 and Figure 6 As shown, Figure 5 TPE-4TA for Sb 3+ The response Figure 6 For PBET to Sb 5+ The response of the two probes was observed. It can be seen that both probes only exhibited significant fluorescence enhancement for antimony ions in the target valence state, while fluorescence enhancement was observed for other common metal ions (Na+, Na ... + K + Ca 2+ Mg 2+ Fe 3+ Cu 2+ Pb 2+ Zn 2+(etc.) showed almost no obvious response, indicating that the probe has good valence state recognition ability and metal ion selectivity. In summary, TPE-4TA showed good valence state recognition ability and metal ion selectivity for Sb. 3+ The signal is strongest for Sb, while other ions show almost no response; PBET for Sb 5+ The signal is strongest, and it has some response to As(V), but its response to As(V) is much lower than that to Sb. 5+ The response of ion 1 was observed, while the responses of other ions were not obvious.

[0062] Metal ion anti-interference experiment

[0063] Fixed Sb 3+ or Sb 5+ The concentration was 50 μg / L, and 10 times the concentration of interfering ions (Pb) was added to the reaction system. 2+ Cd 2+ Hg 2+ Cu 2+ Zn 2+ Fe 3+ Al 3+ And As 5+ (e.g., metal solutions). Results are as follows: Figure 7 and Figure 8 As shown, Figure 7 TPE-4TA probe for trivalent antimony (Sb) 3+ ) result, Figure 8 For PBET probes for pentavalent antimony (Sb) 5+ The results showed that the concentration of antimony ions was 5 ppb and the concentration of coexisting ions was 50 ppb. It can be seen that the two probes were effective against multiple coexisting ions (Na+, Na ... + K + Ca 2+ Mg 2+ Fe 3+ Cu 2+ Pb 2+ Zn 2+ The fluorescence signal changes were minimal in the presence of antimony ions, with deviations controlled within ±30%, indicating that the probe has good anti-interference ability and detection stability for the target antimony ions. In summary, the fluorescence intensity difference was less than 30% compared to the antimony signal alone, indicating that this method has good anti-interference performance.

[0064] Repeatable experiments

[0065] Under the same conditions, for Sb 3+ With Sb 5+ The solutions (0 and 5 μg / L each) were analyzed five times independently in parallel. Results are as follows: Figure 9 and Figure 10 As shown: Sb 3+ The RSD was within ±30%, and Sb 5+The RSD was within ±30%, indicating good repeatability.

[0066] Example 1

[0067] This embodiment discloses a method for rapid detection of antimony ions, including the following steps:

[0068] Step 1): Sb 3+ Establishing a standard curve

[0069] Take Sb at a concentration of 1-10 mg / L 3+ Standard solution (diluted with deionized water), 10 mL aliquots, were added to the fluorescent probe TPE-4TA (final concentration 10 μM), and the reaction was allowed to proceed for 5 minutes. The emission spectrum was measured at an excitation wavelength of 380 nm, and the fluorescence intensity was recorded at 460 nm. Results are as follows: Figure 1 As shown, fluorescence intensity has a good linear relationship with concentration.

[0070] The fluorescent probe TPE-4TA used was the same as described in ACS Applied Materials & Interfaces (2019, Vol. 11, pp. 29619–29629), entitled "Specific and Quantitative Detection of AlbuMin in Biological Fluids by Tetrazolate-Functionalized Water-Soluble AIEgens".

[0071] Step 2): Sb 5+ Establishing a standard curve

[0072] Take Sb at a concentration of 1-10 mg / L 5+ Standard solutions, 10 mL per aliquot, were prepared by adding the fluorescent probe PBET (final concentration 10 μM) and reacting for 4 minutes. Emission spectra were measured at an excitation wavelength of 380 nm, and fluorescence intensity was recorded at 500 nm. Results are as follows: Figure 2 As shown, fluorescence intensity has a good linear relationship with concentration.

[0073] The fluorescent probe PBET used was described in Bioorganic & Medicinal Chemistry (June 22, 2021, version 1), entitled "A Fluorogenic Labeling of Live Viruses".

[0074] Step 3): Take a river water sample, filter it, and take two 2 mL aliquots of the test solution. Add 20 μL of the fluorescent probe (10 μM) TPE-4TA to the first aliquot of the test solution and mix for 5 minutes. Add 20 μL of the fluorescent probe (10 μM) PBET to the second aliquot of the test solution and mix for 5 minutes.

[0075] Step 4): Under 380nm wavelength light excitation, measure the fluorescence intensity I of the first solution at 460nm. 460 The fluorescence intensity I of the second solution at 500 nm 500 ;

[0076] Step 5): Determine the valence state of antimony ions (Sb) based on the fluorescence signal. 3+ Sb 5+ When neither sample shows fluorescence enhancement, it indicates that the sample does not contain Sb. 3+ and Sb 5+ If the first sample shows enhanced fluorescence, it indicates that the sample contains Sb. 3+ If the second sample shows enhanced fluorescence, it indicates that the sample contains Sb. 5+ If both samples show enhanced fluorescence, it indicates that the samples simultaneously contain Sb. 3+ and Sb 5+ ;

[0077] Step 6): Measure the I 460 and I 500 Substitute the pre-established Sb into each 3+ and Sb 5+ Standard curve, used to calculate Sb in the sample 3+ and Sb 5+ The concentration of Sb was detected. 3+ =2.37 μg / L, Sb 5+ =1.19 μg / L. Antimony in different valence states in river water was first separated by chromatography, and then determined by ICP-MS. The result showed Sb... 3+ =2μg / L, Sb 5+ =1μg / L, with an error of less than 20%.

[0078] Example 2

[0079] This embodiment discloses a rapid method for detecting antimony ions, wherein steps 1) and 2) are the same as in Embodiment 1, and the method further includes the following steps:

[0080] Step 3): To verify the applicability of this detection device in complex industrial environments, a simulated industrial wastewater system was used for testing. This simulated system was configured based on the ionic composition and salinity levels of typical mineral processing and electroplating wastewater, containing multiple coexisting metal ions and anions, and can realistically reflect the matrix characteristics of actual water samples. In addition, Sb was added to the first solution. 3+ Solution, add Sb to the second solution 5+ After filtration, take two 2 mL aliquots of the test solution. Add 20 μL of the 10 μM fluorescent probe TPE-4TA to the first aliquot and mix well. Add 20 μL of the 10 μM fluorescent probe PBET to the second aliquot and mix well.

[0081] Step 4): Under 380nm wavelength light excitation, measure the fluorescence intensity I of the first solution at 460nm. 460 The fluorescence intensity I of the second solution at 500 nm 500 ;

[0082] Step 5): Determine the valence state of antimony ions (Sb) based on the fluorescence signal. 3+ Sb 5+ When neither sample shows fluorescence enhancement, it indicates that the sample does not contain Sb. 3+ and Sb 5+ If the first sample shows enhanced fluorescence, it indicates that the sample contains Sb. 3+ If the second sample shows enhanced fluorescence, it indicates that the sample contains Sb. 5+ If both samples show enhanced fluorescence, it indicates that the samples simultaneously contain Sb. 3+ and Sb 5+ ;

[0083] Step 6): Measure the I 460 and I 500 Substitute the pre-established Sb into each 3+ and Sb 5+ Standard curve, used to calculate Sb in the sample 3+ and Sb 5+ The concentration of Sb was detected. 3+ =12μg / L, Sb 5+ =10 μg / L. Parallel determination was performed using ICP-MS, and the result showed Sb... 3+ =10.43 μg / L, Sb 5+ = 8.52 μg / L, with an error of less than 15%.

[0084] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for rapid detection of antimony ions, characterized in that, Includes the following steps: Step 1: Divide the test solution into two portions. Add the fluorescent probe TPE-4TA to the first portion of the test solution and mix well. Add the fluorescent probe PBET to the second portion of the test solution and mix well. Step 2: Under 380nm wavelength light excitation, measure the fluorescence intensity I of the first solution at 460nm. 460 The fluorescence intensity I of the second solution at 500 nm 500 ; Step 3: Determine whether the test solution contains antimony ions and their valence state based on the fluorescence signal: If neither test solution shows fluorescence enhancement, it indicates that the test solution does not contain Sb. 3+ and Sb 5+ ; If the first test solution shows enhanced fluorescence, it indicates that the test solution contains Sb. 3+ If the second test solution shows enhanced fluorescence, it indicates that the test solution contains Sb. 5+ ; If both test solutions show increased fluorescence, it indicates that the test solutions simultaneously contain Sb. 3+ and Sb 5+ .

2. The method for rapid detection of antimony ions according to claim 1, characterized in that, Before step 1, create Sb respectively. 3+ and Sb 5+ Standard curve.

3. The method for rapid detection of antimony ions according to claim 2, characterized in that, After step 3, the measured I 460 and I 500 Substitute Sb respectively 3+ and Sb 5+ The standard curve was used to calculate the Sb content in the test solution. 3+ and Sb 5+ The concentration.

4. The method for rapid detection of antimony ions according to claim 1, characterized in that, In step 1, the concentration of the fluorescent probe TPE-4TA in the first test solution is 0.01-0.5 μM; And / or, the concentration of the fluorescent probe PBET in the second test solution in step 1 is 0.01-0.5 μM.

5. The method for rapid detection of antimony ions according to claim 1, characterized in that, The fluorescent probe TPE-4TA targets Sb. 3+ The detection linear range is 0–10 mg / L; And / or, the fluorescent probe PBET is effective against Sb 5+ The detection linear range is 0–10 mg / L, and the correlation coefficient of the standard curve is ≥0.

99.

6. The method for rapid detection of antimony ions according to claim 1, characterized in that, Step 1: Add the fluorescent probe TPE-4TA to the first test solution and mix for 5 minutes or more; And / or, in step 1, add the fluorescent probe PBET to the second test solution and mix for 4 minutes or more.

7. The method for rapid detection of antimony ions according to claim 1, characterized in that, The pH of the solution to be tested is 6–8.

8. The application of the rapid antimony ion detection method according to any one of claims 1-7 in the field of antimony ion detection.

9. The application according to claim 8, characterized in that, The method for rapid detection of antimony ions is particularly suitable for the detection of antimony ions in drinking water, surface water, groundwater, and soil extracts.

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