Method for efficiently improving functionalization of agilawood essential oil

Abstract

The invention relates to the field of plant essential oil preparation, and discloses a method for efficiently improving functionalization of agilawood essential oil, which comprises the following steps: carrying out heat treatment on fresh agilawood essential oil to obtain a primary product; the preparation method comprises the following steps: adding polysaccharide and a lignin derivative into water, performing primary mixing to obtain a mixed solution, and performing freeze-drying treatment on the mixed solution to obtain a polysaccharide-lignin derivative compound; and mixing the polysaccharide-lignin derivative compound with the primary product for the second time, and emulsifying through the high-speed shearing machine to obtain the agilawood essential oil emulsion. By reasonably regulating and controlling the heat treatment temperature and the ratio of the polysaccharide-lignin derivative to the agilawood essential oil, the functions of aroma quality, skin permeability, oxidation resistance, antibacterial durability, aroma durability and the like of the agilawood essential oil are improved, and finally a high-quality agilawood essential oil product is obtained.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant essential oil preparation, and in particular to a method for efficiently improving the functionalization of agarwood essential oil. BACKGROUND

[0002] Agarwood essential oil mainly contains sesquiterpenes, sesquiterpene alcohols, sesquiterpene ketones and other sesquiterpenes, and has various biological activities, and is widely used in various products. However, freshly extracted agarwood essential oil often has an unpleasant odor, such as a pungent gasoline smell or a smoky smell. These off-flavors are attributed to chemically unstable sesquiterpenes, including sesquiterpene hydrocarbons or sesquiterpene aldehydes. This unpleasant odor can impair user experience and limit its application in high-end perfumes and aromatherapy.

[0003] In order to eliminate the off-flavors in fresh agarwood essential oil, aging is a traditional method to optimize its aroma by allowing fresh essential oil to naturally oxidize and undergo chemical changes over time. In traditional aging processes, the longer the aging time, the more unpleasant odor in the essential oil can gradually dissipate through long-term oxidation, thereby improving the aroma quality of the essential oil. However, this natural aging process usually takes 3 months or even longer, which is difficult to meet the needs of modern industry for efficiency and scale. In addition, long-term aging treatment can cause the essential oil to oxidize, and the biological activity decreases significantly, such as antioxidant activity, antibacterial activity, etc.

[0004] At the same time, agarwood essential oil has problems such as poor water solubility, poor skin permeability and easy volatility, which seriously restrict its function and wide application. In order to solve these problems and realize its application in aqueous systems, emulsification is one of the key technologies. However, traditional emulsifiers such as Span series, sodium dodecyl sulfate and polysorbate compounds can improve dispersibility, but may be left in the system, accompanied by significant cytotoxicity, allergic reactions and environmental residues. In recent years, green emulsifiers based on polysaccharides such as carboxymethyl chitosan have attracted attention due to their biocompatibility, but their emulsification efficiency is low and the cost is high, which is difficult to meet the needs of large-scale industrialization.

[0005] More importantly, the functionalization of agarwood essential oil should not stop at achieving emulsification. The core active ingredient of sesquiterpenes has potential antioxidant, antibacterial and other biological activities, but in the unprotected state, it is easy to decay quickly due to volatility and oxidation. Traditional emulsification processes are mainly based on physical wrapping, and may introduce harmful residues, not only cannot protect and enhance these inherent activities, but also may even inhibit them. Therefore, the industry urgently needs a method that not only efficiently and greenly overcomes the problems of off-flavors, low emulsification efficiency of agarwood essential oil, but also actively enhances its biological activity through processing. SUMMARY

[0006] The present application aims to provide a method for efficiently improving the functionalization of agarwood essential oil, so as to solve the technical problems of long aging time, activity reduction, easy volatilization, poor skin permeability, poor water solubility, low emulsion system efficiency and environmental pollution of the agarwood essential oil in the prior art.

[0007] To solve the above technical problems, the present application specifically provides the following technical solutions:

[0008] The present application provides a method for efficiently improving the functionalization of agarwood essential oil, comprising the following steps:

[0009] S100, heat treating fresh agarwood essential oil to remove the odor sesquiterpenes in the agarwood essential oil, increase the content of sweet and woody components in the essential oil, and obtain a primary product;

[0010] S200, putting polysaccharides and lignin derivatives into water for the first mixing to obtain a mixed solution, and freeze-drying the mixed solution to obtain a polysaccharide-based lignin derivative complex, which is an emulsifier of the primary product;

[0011] S300, secondly mixing the polysaccharide-based lignin derivative complex with the primary product, and performing emulsification treatment by a high-speed shearing machine to obtain an agarwood essential oil emulsion.

[0012] As a preferred scheme of the present application, in the step S100, the heat treatment is heating reflux or microwave heating.

[0013] As a preferred scheme of the present application, the heating temperature of the heating reflux is 150-230 DEG C.

[0014] The heating time is 10-150 min.

[0015] As a preferred scheme of the present application, the heating power of the microwave heating is 300-1000 W, and the heating time is 5-45 min.

[0016] As a preferred scheme of the present application, in the step S200, the polysaccharides are any one or more of hyaluronic acid, sodium alginate, carboxymethyl cellulose, carboxymethyl chitosan, pectin, glucose and gum arabic.

[0017] The lignin derivatives are any one or more of phenolic lignin, sodium lignosulfonate, alkylated lignin, carboxymethylated lignin, hydroxypropyl lignin, nano lignin, aminated lignin and grafted copolymerized lignin.

[0018] As a preferred scheme of the present application, in the step S200, in the first mixing process, the mass ratio of the polysaccharide to the lignin derivative is 1:1-1:5.

[0019] As a preferred scheme of the present application, in the step S300, in the second mixing process, the mass ratio of the polysaccharide-based lignin derivative complex to the primary product is 1:100-1:700.

[0020] As a preferred scheme of the present application, in the step S300, the emulsification process satisfies the following conditions: the rotating speed of the high-speed shearing machine is 8000 r / min-18000 r / min, and the emulsification time is 1-6 min.

[0021] As a preferred scheme of the present application, in the step S100, the preparation of fresh agarwood essential oil is further included, comprising the following steps:

[0022] S110, water is added to the agarwood powder, and steam distillation is performed to separate the essential oil in an oil-water separator, and fresh extracted agarwood essential oil is obtained after drying treatment.

[0023] As a preferred scheme of the present application, in the step S110, the particle size of the agarwood powder is 10-120 mesh, the solid-liquid ratio of the agarwood powder to water is 1:8-20, the distillation time is 6 h, and the drying treatment is anhydrous sodium sulfate or dehydration drying by a reduced pressure distillation instrument.

[0024] Compared with the prior art, the present application has the following beneficial effects:

[0025] The present application accelerates the traditional aging process by heat treatment, promotes the oxidation process of agarwood essential oil, reduces the content of unstable odor components, and increases the content ratio of 11 kinds of wood and sweet aroma components, so that the aroma of the essential oil is much richer than that of the aging treatment, and the aroma quality of the agarwood essential oil is improved.

[0026] The present application first proposes to use a polysaccharide-based lignin derivative complex as an emulsifier, which can realize efficient emulsification of agarwood essential oil by reducing surface tension. The cross-linked network system formed by the complex may interact with the essential oil components through non-covalent interactions (such as hydrogen bonds, van der Waals forces, etc.), which can effectively lock the essential oil components and enhance the stability of the emulsion. In addition, the increase of the absolute value of zeta potential further increases the electrostatic repulsion between emulsion particles, inhibits the aggregation of particles, and makes the emulsion particles reach the nanometer level, further improving the long-term stability of the emulsion. The present application realizes the improvement of the skin permeability, antioxidant property (up to 100%), antibacterial durability, and aroma durability of agarwood essential oil. At the same time, the emulsifier has good biocompatibility and biodegradability, which meets the green and environmental protection concept.

[0027] The optimization process is simple, does not need complex equipment or harsh conditions, reduces production cost, and is suitable for large-scale industrial production. BRIEF DESCRIPTION OF DRAWINGS

[0028] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the following embodiment or prior art description will be briefly introduced. Obviously, the drawings in the following description are only exemplary, and other drawings can be obtained by the provided drawings without creative labor for those skilled in the art.

[0029] Figure 1 A flowchart of the method for efficiently improving the function of agarwood essential oil provided by the present application is shown in the figure.

[0030] Figure 2 A color comparison real object diagram of agarwood essential oil in the embodiment provided by the present application is shown in the figure.

[0031] Figure 3 A surface tension statistical diagram of carboxymethyl chitosan, sodium lignosulfonate, and carboxymethyl chitosan-sodium lignosulfonate complex provided by the present application is shown in the figure.

[0032] Figure 4 A potential value statistical diagram of carboxymethyl chitosan, sodium lignosulfonate, and carboxymethyl chitosan-sodium lignosulfonate complex provided by the present application is shown in the figure.

[0033] Figure 5 A comparison real object diagram of carboxymethyl chitosan-sodium lignosulfonate complex emulsified agarwood essential oil in Example 1 provided by the present application is shown in the figure.

[0034] Figure 6 An antioxidant effect statistical diagram of agarwood essential oil and agarwood emulsion provided by the present application is shown in the figure.

[0035] Figure 7 A permeability effect real object comparison diagram of agarwood essential oil and agarwood emulsion provided by the present application is shown in the figure.

[0036] Figure 8 A fragrance persistence comparison real object diagram of agarwood essential oil and agarwood emulsion provided by the present application is shown in the figure.

[0037] Figure 9 An antibacterial performance comparison real object diagram of agarwood essential oil and emulsion exposed in air for 60 days provided by the present application is shown in the figure.

[0038] Figure 10 An emulsification effect comparison real object diagram of carboxymethyl chitosan-sodium lignosulfonate complex and agarwood essential oil with different proportions provided by the present application is shown in the figure. DETAILED DESCRIPTION

[0039] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0040] like Figure 1 As shown, the present invention provides a method for efficiently enhancing the functionalization of agarwood essential oil, comprising the following steps:

[0041] S100. Heat-treat fresh agarwood essential oil to remove off-odor sesquiterpenes and increase the content of sweet and woody aroma components in the essential oil to obtain a primary product.

[0042] S200. Add polysaccharides and lignin derivatives to water for the first mixing to obtain a mixed solution. Freeze-dry the mixed solution to obtain a polysaccharide-lignin derivative complex. The polysaccharide-lignin derivative complex is used as an emulsifier for the primary product.

[0043] S300: The polysaccharide-lignin derivative complex is mixed with the primary product for a second time, and then emulsified by a high-speed shearing machine to obtain agarwood essential oil emulsion.

[0044] In this invention, heat treatment accelerates traditional aging processes, promotes the oxidation of agarwood essential oil, reduces the content of unstable odor components, and increases the content of woody and sweet-smelling components. Furthermore, by increasing the content of woody and sweet-smelling components, the aroma of the essential oil is far richer than that obtained through aging. Therefore, heat treatment not only shortens the lengthy time required for aging to eliminate odors but also enriches the aroma properties of agarwood essential oil.

[0045] Furthermore, during the emulsification process, the polysaccharide-lignin derivative achieves efficient emulsification of agarwood essential oil by reducing surface tension. Simultaneously, the cross-linked network system formed by this complex may help effectively lock in the essential oil components through non-covalent interactions (such as hydrogen bonds and van der Waals forces), thereby enhancing the emulsion's stability. In addition, the increase in the absolute value of the zeta potential further increases the electrostatic repulsion between emulsion particles, inhibiting particle aggregation and further improving the long-term stability of the emulsion.

[0046] In addition, the raw materials for polysaccharides and lignin derivatives are all derived from natural substances, have good biocompatibility and biodegradability, conform to the concept of green environmental protection, and avoid the environmental pollution and health risks that may be caused by traditional chemically synthesized materials.

[0047] The optimized process proposed in this invention is simple, requires no complex equipment or harsh conditions, reduces production costs, and is suitable for large-scale industrial production.

[0048] Fresh agarwood essential oil can be obtained using readily available methods. Specifically, step S100 also includes the preparation of fresh agarwood essential oil, comprising the following steps:

[0049] S110. Add water to agarwood powder and steam distill to separate the essential oil in an oil-water separator. After drying, freshly extracted agarwood essential oil is obtained.

[0050] In step S110, the ethanol extract content of agarwood powder is 10-30%, the particle size is 10-120 mesh, the material-liquid ratio is 1:8-20, the distillation time is 6 hours, and the drying treatment is anhydrous sodium sulfate or vacuum distillation.

[0051] The heat treatment can be selected from a wide range of options. In step S100, the heat treatment is either reflux heating or microwave heating.

[0052] The heating and reflux time can be selected within a wide range. The heating and reflux time used in this invention is 10 min to 150 min, and the heating temperature is 150℃ to 230℃.

[0053] The processing conditions for microwave heating can be selected within a relatively wide range. Preferably, the heating power of microwave heating is 300-1000W and the time is 5 min-45 min.

[0054] The polysaccharides can be selected from a wide range of options. Preferably, the polysaccharides include any one or more of the following: hyaluronic acid, sodium alginate, carboxymethyl cellulose, carboxymethyl chitosan, pectin, glucose, and gum arabic.

[0055] The lignin derivatives can be selected from a wide range of options. Preferably, the lignin derivatives are any one or more of the following: phenolic lignin, sodium lignin sulfonate, alkylated lignin, carboxymethylated lignin, hydroxypropyl lignin, nano-lignin, aminated lignin, and graft copolymerized lignin.

[0056] The ratio of polysaccharide to lignin derivative can be selected within a wide range. Preferably, during the first mixing process, the ratio of polysaccharide to lignin derivative is 1:1 to 1:5 by mass.

[0057] During the initial mixing process, the polysaccharide reacts with the lignin derivative to form an emulsifier polysaccharide-lignin derivative complex. This complex, with its cross-linked network system, effectively locks in the essential oil components through non-covalent interactions (such as hydrogen bonds and van der Waals forces), thereby enhancing the emulsion's stability. Furthermore, the polysaccharide-lignin derivative complex lowers the zeta potential of the essential oils, further increasing the electrostatic repulsion between emulsion particles and inhibiting particle aggregation, thus improving the long-term stability of the emulsion.

[0058] The ratio of the polysaccharide-lignin derivative complex to the heat-treated agarwood essential oil can be selected within a wide range. Preferably, during the second mixing process, the ratio of the polysaccharide-lignin derivative complex to the primary product is 1:100-1:700 by mass.

[0059] The emulsification conditions can be selected within a relatively wide range. The emulsification conditions are as follows: the speed of the high-speed shearing machine is 8000 r / min-18000 r / min, and the emulsification time is 1-6 min.

[0060] The following examples illustrate the functional principles of heat treatment and emulsifiers:

[0061] Example 1:

[0062] Material selection: Agarwood chips are made from agarwood that forms one year after drilling holes in the agarwood tree, with an ethanol extract content of 22%.

[0063] Operating steps:

[0064] 1. Pulverize dried agarwood chips to 60 mesh to obtain agarwood powder. The ratio of agarwood to water is 1:15. Heat to a gentle boil and extract for 6 hours. The essential oil is separated in an oil-water separator. The content of the ethanol extract is 30%. After drying with anhydrous sodium sulfate, fresh agarwood essential oil is obtained.

[0065] 2. Heat fresh agarwood essential oil to 210℃ using the reflux method, and collect the agarwood essential oil after 1 hour;

[0066] 3. Carboxymethyl chitosan and sodium lignin sulfonate were dissolved in water at a ratio of 1:1.5, mixed evenly, and then freeze-dried to obtain a carboxymethyl chitosan-sodium lignin sulfonate complex for emulsifying agarwood essential oil.

[0067] 4. The ratio of carboxymethyl chitosan-sodium lignin sulfonate compound to heat-treated and optimized agarwood essential oil is 1:300. The mixture is emulsified for 2 minutes at 12000 r / min using a high-speed shearing machine to obtain the agarwood essential oil emulsion.

[0068] Comparative Example 1:

[0069] Take the fresh agarwood essential oil from Example 1.

[0070] Comparative Example 2:

[0071] The other operating steps are the same as in Example 1, except that steps 3 and 4 are not used. Instead, the fresh essential oil in step 2 is aged by placing the agarwood essential oil in the air with the bottle mouth open and a 0.22μm filter membrane placed on top for 6 months.

[0072] Comparative Example 3:

[0073] The other operating steps are the same as in Example 1, except that step 3 is not used. Instead, carboxymethyl chitosan is mixed with heat-treated agarwood essential oil, wherein the ratio of carboxymethyl chitosan to agarwood essential oil by weight is 1:300.

[0074] Comparative Example 4:

[0075] The other operating steps are the same as in Example 1, except that step 3 is not used. Instead, sodium lignosulfonate is mixed with heat-treated agarwood essential oil, wherein the ratio of sodium lignosulfonate to agarwood essential oil by weight is 1:300.

[0076] Results analysis:

[0077] The aroma and component ratios of the essential oils in the above examples and comparative examples were extracted using chemical analysis methods, and data analysis was performed. The aroma and property intensity of each agarwood essential oil are shown in Table 1.

[0078] Table 1 Aroma Properties and Intensity of Agarwood Essential Oil

[0079]

[0080] The aroma components of each agarwood essential oil are shown in Table 2:

[0081] Table 2. Aroma components of agarwood essential oil

[0082]

[0083]

[0084]

[0085]

[0086]

[0087]

[0088]

[0089]

[0090]

[0091]

[0092]

[0093]

[0094]

[0095]

[0096] Fresh agarwood essential oil contains unstable sesquiterpenes, which often have a pungent odor. Conventional aging processes require too much time, making them unsuitable for large-scale production. Heat treatment, however, can accelerate the aging process through high temperatures, stabilizing unstable components. Furthermore, high temperatures can trigger other reactions, introducing components different from those obtained through traditional aging methods, thus enriching the aroma.

[0097] pass Figure 2 As can be seen, compared with Comparative Example 1, in Example 1, the essential oil turned red after heat treatment. Furthermore, like Comparative Example 2 (aging treatment), heat treatment significantly reduced the intensity of the pungent odor, while also increasing the desirable sweet aroma properties. Unlike Comparative Example 2, Example 1 also significantly increased the woody aroma (Table 1). This demonstrates that heat treatment can effectively shorten the long aging time and result in superior aroma properties.

[0098] Compared with Comparative Example 1, the increase in sweet and woody aroma in Example 1 is mainly due to the significant increase in the content of 11 woody or sweet aroma components. Four of the components were added in both Example 1 and Comparative Example 2: 4a,8-dimethyl-2-(prop-1-en-2-yl)-1,2,3,4,4a,5,6,7-octahydronaphthalene (spicy, gum, woody), arugulaene oxide (mint, woody), γ-caryophyllene (warm, woody), and epi-γ-eucalyptol (sweet, woody). These components showed some similarity. The other seven components, such as 4a,5-dimethyl-3-(prop-1-en-2-yl)-1,2,3,4,4a,5,6,7-octahydronaphthalene-1-phenol (woody), rutinol (woody), eucalyptol (woody), cis,α-santalol (sandalwood), β-eudesmene (mint, sweet), β-vetivenene (sweet, woody), and α-guaiacol (sweet, woody), were added in Example 1 but not in Comparative Example 2, demonstrating the uniqueness of heat treatment.

[0099] Furthermore, by comparing Example 1 with Comparative Examples 3-4, it can be seen that the carboxymethyl chitosan-sodium lignin sulfonate complex in Example 1 can effectively emulsify agarwood essential oil, while Comparative Examples 3 and 4 cannot achieve emulsification of agarwood essential oil.

[0100] The surface tension of the carboxymethyl chitosan-sodium lignin sulfonate complex was characterized, and the results were compared with those of pure carboxymethyl chitosan and sodium lignin sulfonate. Figure 3 As shown; a statistical chart comparing its potential with the two is shown below. Figure 4 As shown.

[0101] As can be seen from the figure, the carboxymethyl chitosan-sodium lignosulfonate complex has low surface tension and low potential.

[0102] The significant reduction in surface tension means that the complex can be efficiently adsorbed at the oil-water interface, greatly reducing the interfacial energy, thereby greatly promoting the emulsification process and enabling the formation of emulsions with smaller particle size and more uniform distribution under the same shear conditions.

[0103] Meanwhile, the cross-linked network system formed by this complex may help to effectively lock in the essential oil components through non-covalent interactions (such as hydrogen bonds, van der Waals forces, etc.), thereby enhancing the stability of the emulsion.

[0104] Furthermore, the increase in the absolute value of the zeta potential signifies a fundamental shift in the primary stabilization mechanism of the emulsion—from traditional, relatively fragile electrostatic repulsion stabilization to steric hindrance and interfacial film mechanical stabilization dominated by a dense three-dimensional network formed by the complex at the interface and in the aqueous phase. The increase in the absolute value of the zeta potential further enhances the electrostatic repulsion between emulsion particles, inhibiting particle aggregation and further improving the long-term stability of the emulsion.

[0105] exist Figures 5-9 In comparison with the unemulsified agarwood essential oil in Comparative Example 1, the agarwood essential oil emulsified by this compound showed significant improvements in permeability, antioxidant properties, aroma longevity, and antibacterial longevity.

[0106] The results show that the above-mentioned "interface structuring" and "network trapping" effects can not only strongly prevent droplet aggregation and maturation, but also effectively encapsulate active ingredients such as agarwood essential oil (active ingredient sesquiterpenoids), inhibiting their volatilization and migration. This synergistically solves the stability bottleneck of highly volatile oil phases in applications. The prepared agarwood essential oil emulsion can exhibit superior antioxidant activity, antibacterial durability, and aroma stability compared to the original essential oil, thus breaking through the current application bottlenecks in high-end cosmetics, pharmaceutical preparations, and other fields.

[0107] Among them, the carboxymethyl chitosan-sodium lignosulfonate complex can reduce the size of emulsion particles to the nanoscale, allowing the emulsion particles to penetrate into the skin texture, thereby improving the skin permeability of essential oils. Secondly, the antioxidant capacity of sodium lignosulfonate endows the emulsion with strong antioxidant properties.

[0108] The slow-release active ingredients in the lotion make the aroma and antibacterial properties of the essential oil last longer.

[0109] The following examples further demonstrate the effects of different heating and emulsification conditions on essential oils:

[0110] Example 2

[0111] The other conditions are the same as in Example 1, except that in step 2, the fresh agarwood essential oil is heated to 180°C using a reflux heating method, and the agarwood essential oil is collected after 1 hour.

[0112] Example 3

[0113] The other conditions are the same as in Example 1, except that in step 2, the fresh agarwood essential oil is heated to 200°C using a reflux heating method, and the agarwood essential oil is collected after 1 hour.

[0114] Example 4

[0115] The other conditions are the same as in Example 1, except that the ratio of carboxymethyl chitosan-sodium lignin sulfonate to agarwood essential oil in step 4 is 1:400.

[0116] Example 5

[0117] The other conditions are the same as in Example 1, except that the ratio of the carboxymethyl chitosan-sodium lignin sulfonate complex to agarwood essential oil in step 4 is 1:600.

[0118] Example 6

[0119] The other conditions are the same as in Example 1, except that the ratio of the carboxymethyl chitosan-sodium lignin sulfonate complex to agarwood essential oil in step 4 is 1:700.

[0120] Comparative Example 5

[0121] The other conditions are the same as in Example 1, except that in step 2, the fresh essential oil is aged by placing the agarwood essential oil in the air with the bottle mouth open and a 0.22μm filter membrane placed on top for 3 months.

[0122] Comparative Example 6

[0123] The other conditions are the same as in Example 1, except that the ratio of the carboxymethyl chitosan-sodium lignin sulfonate complex to agarwood essential oil in step 4 is 1:800.

[0124] Comparative Example 7

[0125] The other conditions are the same as in Example 1, except that the ratio of the carboxymethyl chitosan-sodium lignin sulfonate complex to agarwood essential oil in step 4 is 1:900.

[0126] Results analysis:

[0127] The aroma properties and intensity of agarwood essential oil under different treatments are shown in Table 3:

[0128] Table 3 Aroma properties and intensity of agarwood essential oil under different treatments

[0129]

[0130] As can be seen from the data in Table 3, as the heat treatment temperature increases, the pungent odor gradually weakens, while the sweet and woody aromas gradually strengthen.

[0131] This invention effectively reduces pungent odors while enhancing the intensity of sweet and woody aromas by controlling the heat treatment temperature, thereby obtaining agarwood essential oil with high aroma quality.

[0132] Figure 10 The images shown are of the emulsification results for the examples and comparative examples. Figure 10 It can be seen that when the ratio of polysaccharide-lignin derivative complex to agarwood essential oil is within the range of 1:700, the emulsion can be completely emulsified.

[0133] This invention provides key parameters for achieving complete emulsification of agarwood essential oil. By precisely controlling the ratio of polysaccharide-lignin derivatives to agarwood essential oil, the emulsion can achieve the best emulsification effect within a range of 1:700, thereby optimizing the application performance of agarwood essential oil.

[0134] Therefore, by rationally controlling the heat treatment temperature and the ratio of polysaccharide-lignin derivatives to agarwood essential oil, this invention improves the aroma quality, skin permeability, antioxidant, antibacterial durability, and aroma persistence of agarwood essential oil, ultimately obtaining a high-quality agarwood essential oil product.

[0135] This invention addresses two major challenges in the existing technology: freshly extracted agarwood essential oil has a pungent odor that masks other aroma properties; and agarwood essential oil has poor water solubility and permeability, making it difficult to utilize effectively. The invention proposes an innovative method to efficiently enhance the functionality of agarwood essential oil.

[0136] This method, through a process of heat treatment followed by complex emulsification, successfully solved the off-odor problem of fresh agarwood essential oil and significantly improved the aroma quality of the essential oil.

[0137] Furthermore, the complex network proposed in this invention has excellent emulsifying properties and good emulsion stability. It significantly improves the dispersibility of agarwood essential oil in water and significantly enhances its multiple functions, such as skin permeability, antioxidant properties, aroma persistence, and antibacterial persistence. This effectively overcomes many difficulties in the practical application of agarwood essential oil and significantly improves its comprehensive functions, enabling it to be better applied in multiple fields such as skin care and medicine.

[0138] Another significant advantage of this invention is its green and environmentally friendly characteristics. It is easy to operate and leaves no organic solvent residue, which meets the requirements of modern environmental protection and sustainable development.

[0139] This method optimizes the aroma function of agarwood essential oil through heat treatment and emulsification to enhance its bioactivity and other functions, thereby improving both the aroma and bioactivity of the essential oil. This greatly expands and enhances the application scope and effects of agarwood essential oil, providing strong technical support for the development of related fields.

[0140] The above embodiments are merely exemplary embodiments of this application and are not intended to limit this application. The scope of protection of this application is defined by the claims. Those skilled in the art can make various modifications or equivalent substitutions to this application within its substance and scope of protection, and such modifications or equivalent substitutions should also be considered to fall within the scope of protection of this application.

Claims

1. A method for efficiently enhancing the functionalization of agarwood essential oil, characterized in that, Includes the following steps: S100. Heat-treat fresh agarwood essential oil to remove off-odor sesquiterpenes from the agarwood essential oil and increase the content of sweet and woody aroma components in the essential oil to obtain a primary product. S200. Polysaccharide and lignin derivative are added to water for the first mixing to obtain a mixed solution. The mixed solution is then freeze-dried to obtain a polysaccharide-lignin derivative complex, wherein the polysaccharide-lignin derivative complex is an emulsifier for the primary product. S300. The polysaccharide-lignin derivative complex is mixed with the primary product for a second time, and then emulsified by a high-speed shearing machine to obtain agarwood essential oil emulsion.

2. The method for efficiently enhancing the functionalization of agarwood essential oil according to claim 1, characterized in that, In step S100, the heat treatment is either reflux heating or microwave heating.

3. The method for efficiently enhancing the functionalization of agarwood essential oil according to claim 2, characterized in that, The heating temperature of the reflux heating is 150℃-230℃; Heating time is 10-150 minutes.

4. The method for efficiently enhancing the functionalization of agarwood essential oil according to claim 2, characterized in that, The microwave heating power is 300-1000W, and the heating time is 5 min-45 min.

5. The method for efficiently enhancing the functionalization of agarwood essential oil according to claim 1, characterized in that, In step S200, the polysaccharide is any one or more of the following: hyaluronic acid, sodium alginate, carboxymethyl cellulose, carboxymethyl chitosan, pectin, glucose, and gum arabic. The lignin derivative is any one or more of phenolic lignin, sodium lignin sulfonate, alkylated lignin, carboxymethylated lignin, hydroxypropyl lignin, nano-lignin, aminated lignin, and graft copolymerized lignin.

6. The method for efficiently enhancing the functionalization of agarwood essential oil according to claim 1, characterized in that, In step S200, during the first mixing process, the ratio of the polysaccharide to the lignin derivative is 1:1 to 1:5 by mass.

7. The method for efficiently enhancing the functionalization of agarwood essential oil according to claim 1, characterized in that, In step S300, during the second mixing process, the ratio of the polysaccharide-lignin derivative complex to the primary product is 1:100-1:700 by mass.

8. The method for efficiently enhancing the functionalization of agarwood essential oil according to claim 1, characterized in that, In step S300, the emulsification process meets the following conditions: the rotation speed of the high-speed shearing machine is 8000 r / min-18000 r / min, and the emulsification time is 1-6 min.

9. The method for efficiently enhancing the functionalization of agarwood essential oil according to claim 1, characterized in that, Step S100 also includes the preparation of fresh agarwood essential oil, comprising the following steps: S110. Add water to agarwood powder and steam distill to separate the essential oil in an oil-water separator. After drying, freshly extracted agarwood essential oil is obtained.

10. A method for efficiently enhancing the functionalization of agarwood essential oil according to claim 9, characterized in that, In step S110, the particle size of the agarwood powder is 10-120 mesh, the ratio of agarwood powder to water is 1:8-20, the distillation time is 6 hours, and the drying process is dehydration and drying using anhydrous sodium sulfate or a vacuum distillation apparatus.

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