Air-blowing OCE-OCTA-speckle multi-mode elastic imaging device and air-blowing OCE-OCTA-speckle multi-mode elastic imaging method
By using the air-blown OCE-OCTA-speckle multimodal elastography device, which combines the air-blown excitation component and the OCTA signal excitation component, simultaneous imaging of the vascular structure and elastic distribution of brain tissue is achieved. This solves the problem of the difficulty in accurately defining the boundary between lesions and healthy tissue in existing technologies, and provides the independence and accuracy of multi-parameter detection.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- NANCHANG HANGKONG UNIVERSITY
- Filing Date
- 2026-03-16
- Publication Date
- 2026-04-10
AI Technical Summary
Existing single imaging modalities are insufficient to provide comprehensive and accurate pathological information on gliomas and cerebral hemorrhages, especially since the boundary between the tumor area and normal brain tissue is difficult to define precisely, and blood flow information sometimes fails to reflect the tissue perfusion status and microcirculation function.
The OCE-OCTA-speckle multimodal elastography device was used to excite the sample simultaneously by the speckle signal excitation component and the OCTA signal excitation component, combined with the airflow impact of the air excitation component, to achieve in-situ synchronous imaging of the vascular structure and elastic distribution of brain tissue, and to obtain information on vascular flow velocity, distribution and elasticity.
It enables in-situ simultaneous acquisition of microvascular distribution, blood flow velocity, and elastic modulus in brain tissue, accurately pinpointing the physical boundary between lesions and healthy tissue, providing independence and accuracy for multi-parameter detection, and supporting the diagnosis of clinical brain diseases.
Smart Images
Figure CN121817816A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of biomedical photonics imaging, in particular to a gas blowing OCE-OCTA-speckle multimodal elastic imaging device and method. BACKGROUND
[0002] Glioma is the most common primary malignant tumor in the intracranial, with the characteristics of infiltrative growth, resulting in the boundary between tumor tissue and normal brain tissue often blurred. In neurosurgery, how to maximize the resection of the tumor while protecting the normal brain function area (i.e. "maximum safe resection") is the key to improve the survival rate and postoperative quality of life of patients. In addition, cerebral hemorrhage as a high disability rate and high mortality rate of cerebrovascular disease, the assessment of the damage of the brain tissue around the hematoma and the monitoring of the microcirculation state are also crucial for the development of treatment plan.
[0003] At present, a single imaging modality often cannot provide comprehensive and accurate pathological information. For brain glioma, the tumor area is usually accompanied by abnormal angiogenesis, and the microvascular density, morphological distribution and hemodynamic characteristics of the tumor area are significantly different from those of normal brain tissue. Blood flow distribution (vessel morphology) can directly reveal the high metabolic area of the tumor, and the quantitative information of blood flow velocity can reflect the perfusion state and microcirculation function of the tissue. By obtaining these two parameters at high resolution, the lesion area can be more sensitively identified, helping doctors to judge the "functional partitioning" of the lesion and healthy area from the perspectives of metabolism and microcirculation. However, relying solely on blood flow information sometimes cannot accurately define the infiltrating edge, because the blood flow changes of part of the tumor edge may lag behind cell infiltration. SUMMARY
[0004] The purpose of the present application is to provide a gas blowing OCE-OCTA-speckle multimodal elastic imaging device and method, which can in-situ and synchronously image the brain tissue vascular structure and elastic distribution, and provide accurate multimodal brain tissue information.
[0005] To achieve the above-mentioned purpose, the present application provides the following solutions: In a first aspect, the present application provides a gas blowing OCE-OCTA-speckle multimodal elastic imaging device, comprising: a control component, configured to: in a first mode, close the gas blowing excitation component, generate a first instruction and a second instruction to make the speckle signal excitation component and the OCTA signal excitation component synchronously excite the sample; in a second mode, close the speckle signal excitation component, generate a second instruction and a third instruction to make the OCTA signal excitation component and the gas blowing excitation component cooperatively detect; and generate a fourth instruction to adjust the scanning position of the sample scanning component; The speckle signal excitation assembly and the OCTA signal excitation assembly both emit light beams, and the light beams are incident on the sample through the sample scanning assembly to excite speckle signals and light scattering signals, respectively. The signal acquisition assembly is configured to acquire the speckle signals, the light scattering signals and the air-blow OCE scattering signals, and transmit the signals to the control assembly. The control assembly is further configured to determine blood vessel flow rate information and blood vessel distribution information according to the synchronously acquired speckle signals and light scattering signals, determine tissue phase changes according to the light scattering signals, calculate blood vessel elasticity information in combination with corresponding air-blow OCE scattering signals, and determine blood vessel multi-modal elasticity information according to the blood vessel flow rate information, the blood vessel distribution information and the blood vessel elasticity information.
[0006] In a second aspect, the present application provides an air-blow OCE-OCTA-speckle multi-modal elasticity imaging method, which is applied to an air-blow OCE-OCTA-speckle multi-modal elasticity imaging device, and the method comprises the following steps. selecting a current mode; When the current mode is a first mode, an air-blow excitation assembly is turned off, a first instruction and a second instruction are generated to synchronize a speckle signal excitation assembly and an OCTA signal excitation assembly to excite a sample, and speckle signals and light scattering signals are synchronously acquired; A fourth instruction of the first mode is generated to adjust a scanning position of a sample scanning assembly. Blood vessel flow rate information and blood vessel distribution information are determined according to the synchronously acquired speckle signals and light scattering signals. When the current mode is a second mode, the speckle signal excitation assembly is turned off, a second instruction and a third instruction are generated to synchronize the OCTA signal excitation assembly and the air-blow excitation assembly to detect and acquire light scattering signals and corresponding air-blow OCE scattering signals. A fourth instruction of the second mode is generated to adjust the scanning position of the sample scanning assembly. Tissue phase changes are determined according to the light scattering signals, and blood vessel elasticity information is calculated in combination with corresponding air-blow OCE scattering signals. Blood vessel multi-modal elasticity information is determined according to the blood vessel flow rate information, the blood vessel distribution information and the blood vessel elasticity information.
[0007] According to the specific embodiments provided in the application, the application discloses the following technical effects: the application synchronously excites the sample by the speckle signal excitation assembly and the OCTA signal excitation assembly through the setting of two modes, and cooperatively detects by the OCTA signal excitation assembly and the air-blowing excitation assembly. Through the mediation of the OCTA signal excitation assembly, the multi-modal field difference is overcome, the in-situ synchronous acquisition and pixel-level registration of the brain tissue microvascular distribution, blood flow velocity and elastic modulus are realized, and then the in-situ synchronous imaging of the brain tissue vascular structure and elastic distribution is realized. The separate setting of the two modes solves the problem of air-blowing mechanical deformation interference speckle signal, and ensures the independence and accuracy of multi-parameter detection. Especially, the elastic imaging realized by the cooperation of the air-blowing excitation assembly and the OCTA signal excitation assembly can provide the physical and mechanical information of the integrity of the microstructure of the tissue, and is used for "structural verification" of the region delimited by blood flow imaging, so as to accurately lock the physical boundary of the lesion and healthy tissue.
[0008] Briefly, the application organically integrates air-blowing OCE, OCTA and scanning speckle imaging technology, and uses the advantages of air-blowing OCE quantification of brain tissue elasticity, OCTA system high-resolution vascular distribution imaging and scanning speckle system blood flow velocity quantification to realize in-situ synchronous imaging of brain tissue vascular structure and elastic distribution, and to quantitatively acquire blood flow velocity, so as to provide scientific basis and technical support for clinical brain disease diagnosis. BRIEF DESCRIPTION OF DRAWINGS
[0009] In order to more clearly illustrate the technical solutions of the embodiments of the application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments. Obviously, the drawings in the following description are only some embodiments of the application, and other drawings can also be obtained by those skilled in the art without any creative effort on the basis of these drawings.
[0010] Figure 1 It is a structural schematic diagram of an air-blowing OCE-OCTA-speckle multi-modal elastic imaging device in an embodiment of the application.
[0011] Reference signs: 1-narrow line width continuous laser, 2-first optical fiber isolator, 3-first collimator, 4-second collimator, 5-first two-phase color mirror, 6-beam splitter, 7-vibrating mirror group, 8-second two-phase color mirror, 9-first detector, 10-ultra-wideband light-emitting diode, 11-second optical fiber isolator, 12-fiber coupler, 13-third collimator, 14-attenuator, 15-flat mirror, 16-scanning lens, 17-fourth collimator, 18-grating, 19-flat convex lens, 20-linear array CCD, 21-time sequence controller, 22-function generator, 23-air-blowing controller, 24-air pump, 25-nozzle, 26-sample, 27-computer. DETAILED DESCRIPTION
[0012] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the scope of protection of the present application.
[0013] Biological tissue mechanical properties (such as elastic modulus / hardness) are a very valuable endogenous contrast mechanism. Glioma tissue usually has different hardness characteristics than normal brain tissue, and the hematoma and surrounding edema tissue of a cerebral hemorrhage area will also exhibit specific elastic changes. Therefore, elastography can provide physical and mechanical information of the integrity of the microstructure of the tissue, and be used for "structural verification" of the area delineated by blood flow imaging, so as to accurately lock the physical boundary of the lesion and healthy tissue.
[0014] Air-puff OCE, as a non-contact high-sensitivity method, is suitable for elastic measurement on delicate brain tissue, but needs to be perfectly registered with structural and functional imaging to maximize its effectiveness.
[0015] Optical coherence tomography angiography (OCTA) is based on the principle of low coherence interference to obtain a tomogram of the depth direction of the blood vessel distribution. By using this technology, the blood vessel distribution image can be reconstructed by scanning.
[0016] Speckle imaging refers to the backscattering of incident light by scattering particles on the surface of the tissue. Due to the difference in optical path difference of different scattered light reaching the imaging surface of the camera, random interference phenomena will occur between different scattered light on the image surface, which is manifested as a granular pattern of light and dark changes in spatial distribution. The movement of scattering particles (red blood cells) causes fluctuations in the intensity of the image speckle. Detecting and analyzing this fluctuation can obtain relevant information about the movement speed of the scattering particles.
[0017] Therefore, the air puff OCE-OCTA-speckle multimodal elastography device used in the present application can not only preliminarily determine the partition of lesions and healthy areas through blood flow distribution and flow rate information, but also can further verify the fine boundary between the two by using elastic information. This comprehensive evaluation of multiple parameters can provide real-time intraoperative guidance or accurate pathological analysis for neurosurgeons, effectively improving the surgical effect and safety of brain tumor resection and cerebral hemorrhage treatment.
[0018] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the present application will be further described in detail below with reference to the drawings and specific embodiments.
[0019] In one exemplary embodiment, as shown in Figure 1 a gas puffing OCE-OCTA-speckle multimodal elastic imaging device is provided, comprising a gas puffing excitation assembly, a speckle signal excitation assembly, an OCTA signal excitation assembly, a sample scanning assembly, a signal acquisition assembly and a control assembly. The speckle signal excitation assembly and the OCTA signal excitation assembly share the sample scanning assembly, and the control assembly comprises a timing controller 21 and a computer 27.
[0020] Specifically, the control assembly is configured to: in a first mode, turn off the gas puffing excitation assembly, generate a first instruction and a second instruction to synchronize the speckle signal excitation assembly and the OCTA signal excitation assembly to excite the sample 26; in a second mode, turn off the speckle signal excitation assembly, generate a second instruction and a third instruction to synchronize the OCTA signal excitation assembly and the gas puffing excitation assembly to detect; and generate a fourth instruction to adjust the scanning position of the sample scanning assembly. In the two modes, the information of the second instruction is set by the relevant technical personnel as needed.
[0021] In one application, the speckle signal excitation assembly and the OCTA signal excitation assembly each emit a light beam, which is incident on the sample 26 through the sample scanning assembly to excite a speckle signal and a light scattering signal, respectively; and the gas puffing excitation assembly sprays a gas flow to impact the sample 26 under the control of the third instruction to excite a gas puffing OCE scattering signal at the sample 26.
[0022] (I) Speckle signal excitation assembly
[0023] The speckle signal excitation assembly comprises a narrow-linewidth continuous laser 1, a first optical fiber isolator 2 and a first collimator 3 arranged in sequence; the narrow-linewidth continuous laser 1 is connected to the timing controller 21 of the control assembly.
[0024] In operation, the narrow-linewidth continuous laser 1 emits a first light beam, which enters the first dichroic mirror 5 through the first optical fiber isolator 2 and the first collimator 3, is refracted by the first dichroic mirror 5, is reflected and deflected by the galvanometer group 7, and is incident on the sample 26 through the second dichroic mirror 8 and the scanning lens 16 to generate a speckle signal at the sample 26.
[0025] (II) OCTA signal excitation assembly
[0026] The OCTA signal excitation assembly comprises an ultra-wideband light-emitting diode 10, a second optical fiber isolator 11, an optical fiber coupler 12, a second collimator 4 and a beam splitter 6 arranged in sequence; the ultra-wideband light-emitting diode 10 is connected to the timing controller 21 of the control assembly.
[0027] In operation, the second light beam emitted by the ultra-wideband light emitting diode 10 enters the first dichroic mirror 5 through the second optical fiber isolator 11, the fiber coupler 12, the second collimator 4 and the beam splitter 6, is refracted by the first dichroic mirror 5, is reflected and deflected by the galvanometer group 7, is focused by the second dichroic mirror 8 and the scanning lens 16 to the sample 26, and generates a light scattering signal at the sample 26.
[0028] In addition, the fiber coupler 12 is specifically a 2 2 fiber coupler, the second optical fiber isolator 11 is connected with the first end of the fiber coupler 12, and the second collimator 4 is connected with the third end of the fiber coupler 12.
[0029] (Three) sample scanning assembly.
[0030] The sample scanning assembly comprises a galvanometer driving board and a first dichroic mirror 5, a galvanometer group 7, a second dichroic mirror 8 and a scanning lens 16 arranged in sequence; the galvanometer group 7 is arranged on the galvanometer driving board, and the galvanometer driving board is connected with the time sequence controller 21 of the control assembly; the galvanometer driving board is used for adjusting the galvanometer group 7 according to the fourth instruction to change the scanning position.
[0031] Specifically, the scanning on the X-Y plane of the sample 26 is realized by the galvanometer group 7; after adjusting the sample height, the scanning on the X-Y plane at different depths of the sample 26 can be realized through this processing.
[0032] (Four) air-blowing excitation assembly.
[0033] The air-blowing excitation assembly comprises a function generator 22, an air-blowing controller 23, an air pump 24 and a nozzle 25 arranged in sequence; the function generator 22 is connected with the time sequence controller 21 of the control assembly.
[0034] In operation, the function generator 22 is triggered according to the third instruction, sends an air-blowing instruction to the air-blowing controller 23, and then controls the air pump 24 and the nozzle 25 to spray air flow to impact the sample 26 and excite the air-blowing OCE scattering signal at the sample 26. Specifically, the function generator 22 provides a TTL signal to receive a synchronous trigger signal from the time sequence controller, ensures the high-precision synchronization in time between the air-blowing excitation and the optical imaging acquisition, and sends a control instruction to the electromagnetic valve driver (i.e. the air-blowing controller 23) when entering the air-blowing OCE elastic detection mode (i.e. the second mode) to activate the high-speed air valve.
[0035] In one specific application, the signal acquisition component is configured to: acquire the speckle signal, the light scattering signal, and the air puff OCE scattering signal, and transmit to the control component. Specifically, the signal acquisition component comprises a reference arm, a grating spectrometer, and a linear array CCD 20. The reference arm and the grating spectrometer are connected to the fiber coupler 12; the grating spectrometer is connected to the linear array CCD 20; and the linear array CCD 20 is connected to the control component.
[0036] The reference arm comprises a third collimator 13, an attenuator 14, and a plane mirror 15 arranged in sequence; the third collimator 13 is connected to the second end of the fiber coupler 12. The grating spectrometer comprises a fourth collimator 17, a grating 18, and a plano-convex lens 19 arranged in sequence; the fourth collimator 17 is connected to the fourth end of the fiber coupler 12; and the plano-convex lens 19 converges the light beam onto the linear array CCD 20.
[0037] Corresponding to the OCTA signal excitation component, when the signal is acquired, the light scattering signal is transmitted to the second collimator 4 through the sample scanning component, then is coherent with the light beam reflected by the reference arm at the fiber coupler 12, enters the grating spectrometer, and is acquired and received by the linear array CCD 20.
[0038] In addition, the signal acquisition component comprises a detector 9 connected to the control component. Corresponding to the speckle signal excitation component, when the signal is acquired, the speckle signal is reflected by the second dichroic mirror 8 into the detector 9 through the scanning lens 16.
[0039] Corresponding to the air puff excitation component, when the signal is acquired, the air puff OCE scattering signal is transmitted to the grating spectrometer by the fiber coupler 12 through the sample scanning component and the second collimator 4.
[0040] The control component is further configured to: determine blood vessel flow rate information and blood vessel distribution information according to the synchronously acquired speckle signal and light scattering signal; determine tissue phase change according to the light scattering signal, and calculate blood vessel elasticity information in combination with the corresponding air puff OCE scattering signal; and determine blood vessel multi-modal elasticity information according to the blood vessel flow rate information, the blood vessel distribution information, and the blood vessel elasticity information.
[0041] In general, the working process of the device of the application comprises: a time sequence controller is connected with the ultra-wideband light-emitting diode 10, the CCD linear array 20, the narrow-line-width continuous laser 1, the detector 9, the galvanometer drive board and the function generator 22 respectively. By adjusting the time sequence controller, the light beams emitted by the speckle signal excitation assembly and the OCTA signal excitation assembly reach the dichroic mirror at the same time, so that the sample is synchronously scanned, excited and collected by the OCTA and the air-blow OCE, and the blood vessel elasticity information and the blood vessel distribution information are obtained. By adjusting the time sequence controller, the air-blow excitation assembly generates a transient air flow to impact the surface of the brain tissue to induce an elastic wave or deformation, and the OCTA signal excitation assembly records the phase change process of the sample by using a phase-sensitive detection technology, and the elastic modulus distribution of the brain tissue is obtained by using an algorithm reconstruction. When the speckle signal excitation assembly is not working, the air-blow excitation assembly excites the air-blow OCE phase Doppler signal, and then the air-blow OCE spectrum signal is collected by the signal collection assembly, and the blood vessel elasticity information is generated in the computer.
[0042] The device of the application organically integrates the air-blow OCE, the OCTA and the scanning speckle imaging technology, and has the following advantages: by using the dichroic mirror beam combination and the common optical path scanning design, the differences in the fields of view of the multiple modalities are overcome, and the in-situ synchronous acquisition and pixel-level registration of the brain tissue microvessel distribution, the blood flow velocity and the elastic modulus are realized; secondly, by using the specific time sequence control strategy, the problem that the air-blow mechanical deformation interferes with the speckle signal is solved, and the independence and accuracy of the multi-parameter detection are ensured. The device of the application can provide reliable scientific basis and technical support for the function partition and physical boundary definition of the brain lesions of brain diseases such as brain glioma and brain hemorrhage.
[0043] Based on the same inventive concept, the embodiment of the application also provides a method. The implementation scheme for solving the problem provided by the method is similar to the implementation scheme described in the above device, and therefore the specific limitations in one or more method embodiments provided below can be referred to the limitations of the device in the above text, which will not be described herein again.
[0044] In an exemplary embodiment, an air-blow OCE-OCTA-speckle multi-modal elastic imaging method is provided, which is applied to the air-blow OCE-OCTA-speckle multi-modal elastic imaging device described above, and comprises: (1) selecting a current mode. Taking the detection of a biological tissue (brain tissue) as an example, a first mode is selected first.
[0045] (2) when the current mode is the first mode, the air-blow excitation assembly is turned off, and a first instruction and a second instruction are generated to synchronously excite the sample by the speckle signal excitation assembly and the OCTA signal excitation assembly, and to synchronously collect the speckle signal and the light scattering signal.
[0046] (3) generating a fourth instruction of the first mode to adjust the scanning position of the sample scanning assembly; specifically, adjusting the position of the galvanometer group by the galvanometer driving board according to the fourth instruction of the first mode to drive the light beam to perform X-Y scanning, and the light beam after focusing by the scanning lens acts on the sample.
[0047] When in the first mode, the microvascular flow velocity distribution and structure synchronous detection are performed, and the air-blowing excitation assembly is not working. Specifically, the time sequence controller issues the first instruction and the second instruction to turn on the narrow-line-width continuous laser and the ultra-wideband light-emitting diode, and simultaneously turn on the detector; the light beam emitted by the narrow-line-width continuous laser is collimated by the first optical fiber isolator and the first collimator, and is refracted (transmitted) by the first dichroic mirror into the galvanometer group, the galvanometer group drives the light beam to perform X-Y scanning, and the light beam after focusing by the scanning lens acts on the sample; among the light signals returned by the sample, the OCTA interference signals return to the line array CCD along the original path; the speckle scattering signals are reflected by the second dichroic mirror into the detector in the return path.
[0048] (4) determining the blood vessel flow velocity information and the blood vessel distribution information according to the synchronously collected speckle signals and light scattering signals. Finally, the computer synchronously processes the two signals to obtain the pixel-level aligned blood vessel structure and flow velocity distribution.
[0049] (5) when the current mode is the second mode, the speckle signal excitation assembly is turned off, and the second instruction and the third instruction are generated to make the OCTA signal excitation assembly and the air-blowing excitation assembly cooperatively detect and collect the light scattering signals and the corresponding air-blowing OCE scattering signals.
[0050] After the first mode is converted into the second mode or positioned to the region of interest, the time sequence controller turns off the narrow-line-width continuous laser (or blocks the light path thereof), and pauses the speckle signal collection to avoid interference on the subsequent OCTA phase detection. Then, the OCTA and air-blowing preparation are started: the ultra-wideband light-emitting diode and the line array CCD are turned on (or kept on), and the standby state of the air-blowing excitation assembly is activated. The galvanometer group is positioned to the to-be-measured point or enters a specific scanning mode.
[0051] At this time, the time sequence controller triggers the function generator to drive the air-blowing controller to make the nozzle generate a short-pulse air flow to impact the sample. Then, the phase change (displacement) of the tissue during the air blowing is collected at high speed.
[0052] (6) generating a fourth instruction of the second mode to adjust the scanning position of the sample scanning assembly.
[0053] (7) determining the tissue phase change according to the light scattering signals, and calculating the blood vessel elasticity information in combination with the corresponding air-blowing OCE scattering signals; specifically, the computer calculates the tissue elasticity modulus, and performs fusion analysis with the blood vessel and flow velocity information obtained in the first step.
[0054] (8) According to the blood vessel flow rate information, the blood vessel distribution information and the blood vessel elasticity information, determine blood vessel multi-modal elasticity information.
[0055] The speckle signal excitation assembly, the OCTA signal excitation assembly and the air puff excitation assembly are used in combination in the present application, and the timing controller controls the timing of each component to realize synchronous acquisition of speckle and OCTA; when the speckle signal excitation assembly is imaging, after passing through the sample scanning assembly, point scanning is performed synchronously with the OCTA system, corresponding signals are collected, and the flow rate and distribution of blood vessels in the detection range are detected; when the air puff excitation assembly is working, the collection of the speckle signal excitation assembly is suspended, and the phase change of the tissue under the air puff is detected by the OCTA signal excitation assembly to calculate the elasticity.
[0056] In summary, the present application utilizes the advantages of air puff OCE in quantifying brain tissue elasticity, OCTA system in high-resolution blood vessel distribution imaging and scanning speckle system in blood flow velocity quantification, to realize in-situ synchronous imaging of brain tissue blood vessel structure and elasticity distribution, and to quantify blood flow velocity, thereby providing scientific basis and technical support for clinical brain disease diagnosis. The present application can be specifically applied to brain science research and clinical diagnosis, such as brain glioma hardness evaluation and cerebral apoplexy blood flow monitoring. In other words, the present application utilizes the advantages of air puff OCE scattering elasticity imaging in high-resolution detection of bulk modulus, OCTA in high-resolution structural imaging and speckle detection in blood flow velocity detection, to realize in-situ synchronous imaging of blood vessel structure and elasticity distribution, and to quantify blood flow velocity, thereby providing scientific basis and technical support for early clinical diagnosis of vascular diseases.
[0057] The technical features of the above embodiments can be combined in any manner. To make the description concise, not all possible combinations of the technical features in the above embodiments are described, however, as long as the combinations of the technical features do not exist contradictions, they should be considered as the scope of the present application.
[0058] The principles and implementation modes of the present application are described by using specific examples in the present application, and the above embodiment descriptions are only used to help understand the method of the present application and its core idea; meanwhile, for those skilled in the art, according to the idea of the present application, the specific implementation modes and application ranges will be changed. In summary, the content of the present application should not be understood as a limitation.
Claims
1. A blown OCE-OCTA-speckle multimodal elastography device, characterized in that, The device includes: The control component is configured to: in a first mode, turn off the air-blowing excitation component, generate a first instruction and a second instruction to enable the speckle signal excitation component and the OCTA signal excitation component to excite the sample synchronously; in a second mode, turn off the speckle signal excitation component, generate a second instruction and a third instruction to enable the OCTA signal excitation component and the air-blowing excitation component to detect together; and generate a fourth instruction to adjust the scanning position of the sample scanning component. The speckle signal excitation component and the OCTA signal excitation component both emit light beams, which are incident on the sample through the sample scanning component, thereby exciting speckle signals and light scattering signals, respectively; the air blowing excitation component ejects airflow to impact the sample under the control of the third command, thereby exciting the air blowing OCE scattering signal at the sample. The signal acquisition component is used to: acquire the speckle signal, the light scattering signal, and the air-blown OCE scattering signal, and transmit them to the control component; The control component is also used to: determine blood vessel flow velocity information and blood vessel distribution information based on the synchronously acquired speckle signal and light scattering signal; determine tissue phase change based on the light scattering signal and calculate blood vessel elasticity information in combination with the corresponding air-blown OCE scattering signal; and determine blood vessel multimodal elasticity information based on the blood vessel flow velocity information, blood vessel distribution information and blood vessel elasticity information.
2. The air-blown OCE-OCTA-speckle multimodal elastography device according to claim 1, characterized in that, The sample scanning assembly includes a galvanometer drive plate and a first dichroic mirror, a galvanometer group, a second dichroic mirror and a scanning lens arranged in sequence. The galvanometer assembly is mounted on the galvanometer drive board, which is connected to the control component. The galvanometer drive board is used to adjust the galvanometer assembly according to the fourth instruction to change the scanning position.
3. The air-blown OCE-OCTA-speckle multimodal elastography device according to claim 2, characterized in that, The speckle signal excitation component includes a narrow linewidth continuous laser, a first fiber isolator, and a first collimator arranged sequentially; the narrow linewidth continuous laser is connected to the control component. The signal acquisition component includes a detector, which is connected to the control component. During operation, the narrow linewidth continuous laser emits a first beam, which passes through the first fiber isolator and the first collimator into the first dichroic mirror. After being refracted by the first dichroic mirror, the beam enters the galvanometer group. After being reflected and deflected by the galvanometer group, the beam passes through the second dichroic mirror and the scanning lens and is incident on the sample, generating a speckle signal at the sample. The speckle signal is reflected by the second dichroic mirror through the scanning lens and into the detector.
4. The air-blown OCE-OCTA-speckle multimodal elastography device according to claim 2, characterized in that, The OCTA signal excitation component includes an ultra-wideband light-emitting diode, a second fiber isolator, a fiber coupler, a second collimator, and a beam splitter arranged sequentially; the ultra-wideband light-emitting diode is connected to the control component. During operation, the ultra-wideband light-emitting diode emits a second beam, which passes through the second fiber isolator, the fiber coupler, the second collimator, and the beam splitter into the first dichroic mirror. After being refracted by the first dichroic mirror, the beam enters the galvanometer group. After being reflected and deflected by the galvanometer group, the beam is focused onto the sample by the second dichroic mirror and the scanning lens, generating a light scattering signal at the sample.
5. The air-blown OCE-OCTA-speckle multimodal elastography device according to claim 4, characterized in that, The signal acquisition components include a reference arm, a grating spectrometer, and a linear CCD array. The reference arm and the grating spectrometer are both connected to the fiber optic coupler; the grating spectrometer is connected to the linear CCD array; and the linear CCD array is connected to the control assembly. During operation, the light scattering signal is transmitted in reverse to the second collimator via the sample scanning component, and then coherently with the light beam reflected by the reference arm at the fiber coupler, entering the grating spectrometer and being acquired and received by the linear CCD.
6. The air-blown OCE-OCTA-speckle multimodal elastography device according to claim 5, characterized in that, The reference arm includes a third collimator, an attenuator, and a plane mirror arranged in sequence; the third collimator is connected to the fiber optic coupler. The grating spectrometer includes a fourth collimator, a grating, and a plano-convex lens arranged in sequence; the fourth collimator is connected to the fiber optic coupler; and the plano-convex lens focuses the light beam onto the linear CCD array.
7. The air-blown OCE-OCTA-speckle multimodal elastography device according to claim 1, characterized in that, The air-blowing excitation assembly includes a function generator, an air-blowing controller, an air pump, and a nozzle arranged in sequence; the function generator is connected to the control assembly. During operation, the function generator is triggered according to the third instruction and sends an air blowing command to the air blowing controller. Then, the air blowing controller controls the air pump and the nozzle to spray airflow to impact the sample and excite the air blowing OCE scattering signal at the sample.
8. The air-blown OCE-OCTA-speckle multimodal elastography device according to claim 5, characterized in that, The air-blown OCE scattering signal is transmitted to the grating spectrometer via the sample scanning component, the second collimator, and then the fiber optic coupler.
9. A blown OCE-OCTA-speckle multimodal elastography method, applied to the blown OCE-OCTA-speckle multimodal elastography device according to any one of claims 1-8, characterized in that, The method includes: Select the current mode; When the current mode is the first mode, the air blowing excitation component is turned off, and the first command and the second command are generated so that the speckle signal excitation component and the OCTA signal excitation component can excite the sample synchronously and acquire speckle signal and light scattering signal simultaneously. Generate a fourth instruction for the first mode to adjust the scanning position of the sample scanning component; Based on the synchronously acquired speckle signals and light scattering signals, the vascular flow velocity information and vascular distribution information are determined; When the current mode is the second mode, the speckle signal excitation component is turned off, and a second command and a third command are generated so that the OCTA signal excitation component and the air blowing excitation component can work together to detect and collect light scattering signals and corresponding air blowing OCE scattering signals. Generate a fourth instruction for the second mode to adjust the scanning position of the sample scanning component; Based on the light scattering signal, the tissue phase change is determined, and combined with the corresponding air-blown OCE scattering signal, the vascular elasticity information is calculated. Based on the blood vessel flow velocity information, blood vessel distribution information, and blood vessel elasticity information, the multimodal elasticity information of blood vessels is determined.
10. The air-blown OCE-OCTA-speckle multimodal elastography method according to claim 9, characterized in that, Generate a fourth instruction for the first mode to adjust the scanning position of the sample scanning component, including: The position of the galvanometer assembly is adjusted by the galvanometer driver board according to the fourth command of the first mode, so as to drive the beam to perform XY scanning, and then apply it to the sample after being focused by the scanning lens.