Water culture method of multicoloured leaves and application of water culture method
By using hydroponics and optimized nutrient solution treatment, the problems of pests and diseases and continuous cropping obstacles in variegated leaves were solved, promoting high-yield and high-quality growth of the plants and improving economic benefits.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
- Filing Date
- 2026-03-31
- Publication Date
- 2026-05-01
AI Technical Summary
Variegated leaves are prone to wilting, frequent pests and diseases, continuous cropping obstacles, and reduced yields under monoculture. Intercropping, on the other hand, involves complex water and fertilizer management and resource requirements, resulting in poor economic benefits.
The seedlings of *Phaeocarpus macrocarpus* were cultured using hydroponics after being treated with carbendazim in a nutrient solution formulated by a Japanese garden experiment. Light and temperature were controlled, and the nutrient solution was changed regularly to optimize growth conditions.
It can improve the growth quality and yield of variegated leaves, enhance the growth of both above-ground and underground parts, and increase economic benefits.
Smart Images
Figure CN121942549A_ABST
Abstract
Description
A hydroponic method for growing variegated leaves and its application Technical Field
[0001] This invention relates to the field of crop cultivation technology, and in particular to a hydroponic method for growing variegated leaves and its application. Background Technology
[0002] Pandanus amaryllifolius, a perennial herbaceous spice and specialty economic crop belonging to the Pandanaceae family and the Pandanus genus, is widely cultivated in Wanning, Qionghai, Danzhou, and other cities and counties in Hainan Province. It is the only Pandanus species with fragrant leaves, making it a natural ingredient in pastries, beverages, ice cream, and other food products. Known as the "Oriental Herb," its applications in food, spices, medicine, and ornamental uses are increasingly widespread, resulting in very high economic development value.
[0003] The cultivation methods for variegated leaves are mainly divided into monoculture and intercropping. In monoculture, the inherent characteristics of variegated leaves, such as being easy to grow, thriving in moist conditions, tolerating high temperatures, and being shade-tolerant, make them prone to wilting under strong sunlight. While intercropping variegated leaves with cash crops such as rubber plantations, coconut plantations, and bananas can increase additional economic value to some extent, it also presents many challenges in terms of water and fertilizer management, water resource requirements, land resource requirements, and crop harvesting, which can easily lead to frequent pests and diseases, continuous cropping obstacles, and reduced yields. Summary of the Invention
[0004] The purpose of this invention is to provide a hydroponic method for cultivating variegated leaves and its application, in order to solve the problems of frequent pests and diseases, continuous cropping obstacles, and reduced yield in the cultivation of variegated leaves in the prior art.
[0005] In order to achieve the above-mentioned invention objectives, the present invention provides the following technical solution: The present invention provides a hydroponic method for variegated leaves, including the following steps: (1) soaking variegated leaf seedlings in carbendazim for 5-15 minutes to obtain sterilized seedlings; (2) placing the sterilized seedlings in hydroponic nutrient solution for 55-70 days to obtain variegated leaf plants.
[0006] Preferably, the concentration of carbendazim in step (1) is 50% carbendazim diluted 800 to 1200 times.
[0007] Preferably, the hydroponic nutrient solution in step (2) includes one or more of the following: Japanese Garden Experimental Formula Nutrient Solution, 1 / 2 Japanese Garden Experimental Formula Nutrient Solution, 1 / 4 Japanese Garden Experimental Formula Nutrient Solution, Hogland Formula Nutrient Solution, 1 / 2 Hogland Formula Nutrient Solution and 1 / 4 Hogland Formula Nutrient Solution.
[0008] Preferably, the culture temperature in step (2) is 25-28°C.
[0009] Preferably, the light intensity for cultivation in step (2) is 30–80 μmol / (m²). 2 s).
[0010] Preferably, step (2) also includes changing the hydroponic nutrient solution every 7 to 21 days.
[0011] The present invention also provides the application of the hydroponic method described above in the cultivation of variegated leaves.
[0012] The present invention has the following technical effects and advantages: the hydroponic method of the present invention and the hydroponic nutrient solution used therein can effectively promote the growth and development of variegated leaves, improve the growth of the above-ground and underground parts of variegated leaves, thereby achieving high-yield and high-quality cultivation of variegated leaves, and improving the variety yield and economic benefits of variegated leaves. Attached Figure Description
[0013] Figure 1 shows the aboveground morphology and growth of each group of variegated leaves plants; Figure 2 shows the underground morphology and growth of each group of variegated leaves plants; Figure 3 shows the pH changes of the hydroponic nutrient solution for each group of variegated leaves plants. Detailed Implementation
[0014] The present invention provides a hydroponic method for variegated leaves, comprising the following steps: (1) soaking variegated leaf seedlings in carbendazim for 5 to 15 minutes, preferably 10 minutes, to obtain sterilized seedlings; (2) placing the sterilized seedlings in a hydroponic nutrient solution for 55 to 70 days, preferably 60 days, to obtain variegated leaf plants.
[0015] In this invention, the carbendazim mentioned in step (1) is preferably a 50% carbendazim diluted 800 to 1200 times, and more preferably a 1000-fold dilution.
[0016] In this invention, the hydroponic nutrient solution in step (2) includes one or more of the following: Japanese Garden Experimental Formula Nutrient Solution, 1 / 2 Japanese Garden Experimental Formula Nutrient Solution, 1 / 4 Japanese Garden Experimental Formula Nutrient Solution, Hogland Formula Nutrient Solution, 1 / 2 Hogland Formula Nutrient Solution and 1 / 4 Hogland Formula Nutrient Solution, preferably Japanese Garden Experimental Formula Nutrient Solution.
[0017] In this invention, the culture temperature in step (2) is 25-28°C, preferably 28°C.
[0018] In this invention, the light intensity for cultivation in step (2) is 30–80 μmol / (m²). 2 s), preferably 60 μmol / (m 2 s).
[0019] In this invention, step (2) also includes changing the hydroponic nutrient solution every 7 to 21 days, preferably every 15 days.
[0020] The present invention also provides the application of the hydroponic method described above in the cultivation of variegated leaves.
[0021] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.
[0022] In the test materials of this invention, the seedlings of *Panax japonicus* were obtained from the Institute of Tropical Crop Germplasm Resources, Chinese Academy of Tropical Agricultural Sciences; in the reagents of this invention, the Hogland formula nutrient solution and the Japanese garden trial formula nutrient solution were purchased from Hunan Hogland Bioengineering Co., Ltd.; in the equipment of this invention, the HIPOINT 740FLED type low temperature plant incubator was purchased from Hainan Bainian Qingniao Technology Service Co., Ltd., the pH-100A type pH meter was purchased from Shanghai Lichen Bangxi Instrument Technology Co., Ltd., and the ST3100C type conductivity meter was purchased from Ohaus Instruments Changzhou Co., Ltd.
[0023] Example 1: Hydroponic cultivation of *Variegata* var. *variegata*. Healthy, uniformly sized rooted tissue culture seedlings were selected and hardened off to obtain *Variegata* seedlings. These seedlings were then soaked in a 1000-fold dilution of 50% carbendazim for 10 minutes to obtain sterilized seedlings. The sterilized seedlings were divided into 7 groups: A1, A2, A3, A4, A5, A6, and CK. These groups were then transplanted into hydroponic containers containing 1 / 4 Hoagland's formula nutrient solution, 1 / 2 Hoagland's formula nutrient solution, 1 / 4 Japanese horticultural formula nutrient solution, 1 / 2 Japanese horticultural formula nutrient solution, and distilled water, respectively. The containers were placed in an HIPOINT 740FLED low-temperature plant incubator at 28℃ and a light intensity of 60 μmol / (m²). 2 The plants were cultured under the following conditions, with the hydroponic nutrient solution being replaced every 15 days. After 60 days of culture, the variegated leaf plants were obtained.
[0024] Experiment Example 1: Effect of hydroponic nutrient solution on the aboveground growth of variegated leaves. The plant height, plant width and number of leaves of each group of variegated leaves in Example 1 were determined, and the results are shown in Table 1 and Figure 1.
[0025] Table 1. Results of aboveground indicators and growth of *Variegata* plants The results showed that the average plant height, average plant width, and average number of leaves in each group of *Variegata variegata* were significantly different from those in the control group (CK). Compared with the CK group, in terms of plant height, the average plant height of group A6 increased by 88.89%, showing the most significant difference, while group A3 increased by 40.89%; in terms of plant width, the average plant width of group A6 increased by 121.61%, showing the most significant difference, while group A2 increased by 53.49%; and in terms of the number of leaves, the average number of leaves of group A6 increased by 78.54%, showing the most significant difference, while group A3 increased by 53.54%. This indicates that the Japanese horticultural formula nutrient solution is more beneficial to the plant height, plant width, and leaf growth of hydroponically grown *Variegata variegata* seedlings than the Hogland formula nutrient solution, and the Japanese horticultural formula nutrient solution has a better effect than the 1 / 2 and 1 / 4 Japanese horticultural formula nutrient solutions.
[0026] Experiment Example 2: Effect of hydroponic nutrient solution on root growth of variegated leaves. The number and length of roots of each group of variegated leaves in Example 1 were determined, and the root growth was evaluated. The results are shown in Table 2 and Figure 2. In the table, "+" indicates weak root growth, "++" indicates relatively strong growth, and "+++" indicates robust growth.
[0027] Table 2 Results of root index measurements of Variegated Leaf plants The results showed that the average number of roots, average root length, and root vigor of each group of *Variegata* plants increased compared to the control group. Groups A1, A5, and A6 showed significant differences compared to the control group, while groups A2 and A4 showed relatively significant differences in average root number and average root length. Compared to the control group, group A6 showed a 36.78% increase in average root number and a 65.73% increase in average root length, with vigorous root growth. This indicates that the nutrient solution formulated in the Japanese garden experiment is the optimal nutrient solution for the root growth of *Variegata* plants in the hydroponic method of this invention.
[0028] Experiment Example 3: Effect of hydroponic nutrient solution on the biomass of variegated leaves. The fresh weight and dry weight of the aboveground parts, underground parts and whole plant of each group of variegated leaves in Example 1 are shown in Table 3.
[0029] Table 3 Results of biomass determination of Variegated Leaf plants The results showed that the A6 group of variegated leaves had the highest fresh weight of above-ground parts, underground parts, and whole plant, which were 16.74 times, 4.57 times, and 8.42 times higher than the CK group, respectively; the above-ground parts, underground parts, and whole plant dry weight were also the highest, which were 13.86 times, 3.45 times, and 6.69 times higher than the CK group, respectively. This indicates that the nutrient solution formulated in the Japanese garden experiment used in the hydroponic method of this invention has the best promoting effect on the growth and development of variegated leaves.
[0030] Experiment 4: Effect of hydroponic nutrient solution on pH of variegated leaves in hydroponics. The pH of the hydroponic nutrient solution in each group was measured using a pH-100A pH meter on days 1, 15, 30, 45 and 60 of hydroponics. The results are shown in Table 4 and Figure 3.
[0031] Table 4. pH Measurement Results of Variegated Leaf Hydroponics The results showed that the pH change of the variegated leaves plants was not significant throughout the entire culture process in distilled water. However, when cultured in hydroponic nutrient solutions in each group, the pH of the hydroponic nutrient solution decreased every 15 days, and the variegated leaves plants grew better than the control group. This indicates that a slightly lower pH in the hydroponic nutrient solution is beneficial to the growth of variegated leaves plants.
[0032] Experiment 5: Effect of hydroponic nutrient solution on the relative permeability of variegated leaf cell membranes. Leaves of variegated leaves from each group in Example 1 were taken, and the conductivity of each leaf was measured using an ST3100C conductivity meter. After boiling each leaf for 30 minutes and cooling it to room temperature, the boiling conductivity of each leaf was measured, and the relative conductivity was calculated. The results are shown in Table 5. The formula for calculating the relative conductivity (REC) is: REC = conductivity / boiling conductivity.
[0033] Table 5. Results of relative permeability measurement of variegated leaf cell membranes The results showed that the CK group had the highest relative conductivity of the leaves, indicating severe cell membrane damage and the greatest electrolyte leakage. The relative conductivity of the leaves in all other groups was significantly lower than that in the CK group, with the A6 group showing the lowest relative conductivity. This indicates that the nutrient solution formulated in the Japanese garden experiment can improve the growth of variegated leaves.
[0034] As can be seen from the above embodiments, the present invention provides a hydroponic method for growing variegated leaves and its application. The hydroponic method and the hydroponic nutrient solution used in the present invention can effectively promote the growth and development of variegated leaf plants and improve the growth of both the above-ground and underground parts of the variegated leaf plants.
[0035] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.
Claims
1. A hydroponic method for growing variegated leaves, characterized in that, The process includes the following steps: (1) Soaking the variegated seedlings in carbendazim for 5-15 minutes to obtain sterilized seedlings; (2) Cultivating the sterilized seedlings in hydroponic nutrient solution for 55-70 days to obtain variegated plantlets.
2. The hydroponic method according to claim 1, characterized in that, The concentration of carbendazim in step (1) is 50% carbendazim diluted 800 to 1200 times.
3. The hydroponic method according to claim 2, characterized in that, The hydroponic nutrient solution mentioned in step (2) includes one or more of the following: Japanese Garden Experimental Formula Nutrient Solution, 1 / 2 Japanese Garden Experimental Formula Nutrient Solution, 1 / 4 Japanese Garden Experimental Formula Nutrient Solution, Hogland Formula Nutrient Solution, 1 / 2 Hogland Formula Nutrient Solution and 1 / 4 Hogland Formula Nutrient Solution.
4. The hydroponic method according to claim 3, characterized in that, The culture temperature in step (2) is 25-28℃.
5. The hydroponic method according to claim 4, characterized in that, The light intensity for cultivation in step (2) is 30–80 μmol / (m²). 2 s).
6. The hydroponic method according to claim 1, characterized in that, Step (2) also includes changing the hydroponic nutrient solution every 7 to 21 days.
7. The application of the hydroponic method according to any one of claims 1 to 6 in the cultivation of variegated leaves.