Reagent recovery fluid system

Abstract

The invention discloses a reagent recovery fluid system. The reagent recovery fluid system comprises a reagent supply assembly, a synthesis assembly and a recovery assembly, the reagent supply assembly comprises a reagent source, a liquid supply pipeline and a reagent driving pump, one end of the liquid supply pipeline is communicated with the reagent source, the reagent driving pump is arranged on the liquid supply pipeline, and the synthesis assembly comprises a synthesis device and a waste discharge pipeline. The other end of the liquid supply pipeline is communicated with an inlet of a synthesis cavity of the synthesis device, one end of the waste discharge pipeline is communicated with a waste discharge port of the synthesis cavity, the recovery assembly comprises a first switching valve and a plurality of waste liquid recovery containers, and the other end of the waste discharge pipeline is communicated with a liquid inlet of the first switching valve; the multiple waste liquid recovery containers are communicated with the multiple liquid outlets of the first switching valve in a one-to-one correspondence mode. The reagent recovery fluid system can automatically switch the vacant waste liquid recovery container when one waste liquid recovery container reaches the preset capacity, and the time consumed for switching the waste liquid recovery container is short.

Description

Technical Field

[0001] This invention relates to the field of nucleic acid synthesis technology, and in particular to a reagent recovery fluid system. Background Technology

[0002] A nucleic acid synthesis system is a device that uses chemical methods to synthesize nucleic acids. In this system, the waste liquid recovery tank is a key component, used to recover the reagent waste liquid generated during the synthesis process. Currently, in nucleic acid synthesis processes, only one waste liquid recovery tank is typically used to collect all the reagent waste liquid. The tank's fullness is usually determined manually based on observation and experience. Once full, the tank is manually disassembled to empty the waste liquid, and then reassembled for further waste liquid collection.

[0003] Manually judging whether the waste liquid recovery tank is full based on experience has low accuracy and poor stability. The method of removing the waste liquid recovery tank to pour out the reagent waste liquid is complicated, requires a lot of manpower, has poor safety, and requires pausing the nucleic acid synthesis reaction. This not only seriously reduces the working efficiency of the device, but also makes it difficult to control the reaction time of the nucleic acid synthesis reaction, resulting in poor nucleic acid synthesis effect.

[0004] Especially for large-scale nucleic acid synthesis reactions, a large amount of reagent waste liquid is generated. Therefore, it is necessary to frequently disassemble the waste liquid recovery tank to prevent overflow. This further amplifies the drawbacks of manually disassembling and emptying the tank, making it increasingly unsuitable for the requirements. Furthermore, since nucleic acid synthesis reactions use chemical reagents that are highly corrosive, flammable, and explosive, it is not feasible to install a level sensor inside the waste liquid recovery tank to monitor whether it is full.

[0005] Therefore, how to propose a recycling system that can not only monitor the amount of reagent waste liquid in real time, but also improve the recycling efficiency of reagent waste liquid to avoid spending a lot of time on dumping reagent waste liquid is an urgent technical problem to be solved. Summary of the Invention

[0006] The purpose of this invention is to provide a reagent recovery fluid system that can monitor the amount of reagent waste liquid in real time and can quickly replace the waste liquid recovery container for collecting reagent waste liquid.

[0007] To achieve this objective, the present invention adopts the following technical solution:

[0008] A reagent recovery fluid system includes: a reagent supply component, comprising a reagent source, a liquid supply pipeline, and a reagent drive pump, one end of the liquid supply pipeline being connected to the reagent source, and the reagent drive pump being disposed on the liquid supply pipeline; a synthesis component, comprising a synthesis device and a waste discharge pipeline, the synthesis device having a synthesis chamber, the other end of the liquid supply pipeline being connected to the inlet of the synthesis chamber, and one end of the waste discharge pipeline being connected to the waste discharge port of the synthesis chamber; and a recovery component, comprising a first switching valve and a plurality of waste liquid recovery containers, the other end of the waste discharge pipeline being connected to the inlet of the first switching valve, and the plurality of waste liquid recovery containers being connected one-to-one to a plurality of outlets of the first switching valve; wherein, when the reagent recovery fluid system detects that the volume of the waste liquid recovery container has reached a predetermined volume, it controls the first switching valve to operate, so that the waste discharge flow route within the first switching valve is switched from the current waste liquid recovery container to an empty waste liquid recovery container.

[0009] Preferably, the reagent driving pump delivers a fixed amount of reagent per pumping cycle, and the reagent recovery fluid system can obtain the total amount of reagent pumped based on the number of pumping cycles of the reagent driving pump, and can control the operation of the first switching valve based on the total amount of reagent and the capacity of the waste liquid recovery container.

[0010] Preferably, the liquid supply pipeline includes a confluence pipeline and at least two branch pipelines, one end of each of the at least two branch pipelines is connected to one end of the confluence pipeline, and the other end of the confluence pipeline is connected to the inlet of the synthesis chamber; at least two reagent sources are provided, and the other ends of the at least two branch pipelines are respectively connected to the at least two reagent sources one by one, and each branch pipeline is provided with a reagent driving pump.

[0011] Preferably, at least one of the plurality of reagent-driven pumps is in operation, and the different reagent sources contain the same or different reagents, including reaction reagents and cleaning reagents.

[0012] Preferably, at least two of the branch pipes are connected to the confluence pipe via a multi-port connector.

[0013] Preferably, the reagent driving pump is a high-pressure constant flow pump; or, the reagent driving pump is an injection dosing pump.

[0014] Preferably, the synthesis device is provided in multiple ways; the synthesis assembly further includes a second switching valve, which is provided with a main inlet, a main outlet and multiple branch flow paths. The main inlet is connected to the other end of the liquid supply pipeline, the main outlet is connected to the waste discharge pipeline, the flow path inlets of the multiple branch flow paths can be selectively connected to the main inlet, the flow path outlets of the multiple branch flow paths can be selectively connected to the main outlet, and the multiple synthesis devices are respectively connected to the multiple branch flow paths one by one through multiple branch pipes.

[0015] Preferably, the branch flow path includes a first flow path and a second flow path. One end of the first flow path is the flow path inlet, and the other end forms a first communication port on the valve body of the second switching valve. One end of the second flow path is the flow path outlet, and the other end forms a second communication port on the valve body of the second switching valve. The branch pipe includes a first pipe and a second pipe. The first pipe connects the inlet of the synthesis chamber and the first communication port, and the second pipe connects the waste outlet of the synthesis chamber and the second communication port.

[0016] Preferably, the waste discharge pipeline is equipped with a flow limiting valve.

[0017] Preferably, the reagent source includes multiple reagent containers, and different reagent containers contain the same or different reagents.

[0018] The beneficial effects of this invention are:

[0019] The reagent recovery fluid system provided by this invention includes a reagent supply component, a synthesis component, and a recovery component. The reagent supply component includes a reagent source, a liquid supply pipeline, and a reagent drive pump. One end of the liquid supply pipeline is connected to the reagent source, and the reagent drive pump is located on the liquid supply pipeline. The synthesis component includes a synthesis device and a waste discharge pipeline. The synthesis device has a synthesis chamber. The other end of the liquid supply pipeline is connected to the inlet of the synthesis chamber, and one end of the waste discharge pipeline is connected to the waste discharge port of the synthesis chamber. The recovery component includes a first switching valve and multiple waste liquid recovery containers. The other end of the waste discharge pipeline is connected to the inlet of the first switching valve, and the multiple waste liquid recovery containers are connected one-to-one to multiple outlets of the first switching valve. When the reagent recovery fluid system detects that the volume in the waste liquid recovery containers has reached a predetermined capacity, it controls the first switching valve to operate, thereby switching the waste discharge flow route within the first switching valve from the current waste liquid recovery container to an empty waste liquid recovery container. This reagent recovery fluid system can monitor the amount of reagent waste liquid in real time and can automatically switch to an empty waste liquid recovery container when a waste liquid recovery container reaches a predetermined capacity. The switching time is short, which helps to improve the system's working efficiency and the success rate of the experiment. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the reagent recovery fluid system provided by the present invention.

[0021] In the picture:

[0022] 10. Reagent supply assembly; 20. Synthesis assembly; 30. Recovery assembly;

[0023] 100. Reagent source; 110. First reagent source; 120. Second reagent source; 200. Liquid supply line; 210. Merging line; 220. Branch line; 221. First branch line; 222. Second branch line; 230. T-connector; 300. Reagent drive pump; 310. First drive pump; 320. Second drive pump; 400. Synthesis device; 410. First synthesis column; 420. Second synthesis column; 430. Third synthesis column; 500. Waste discharge line; 510. Flow limiting valve; 600. First switching valve; 700. Waste liquid recovery container; 710. First waste liquid tank; 720. Second waste liquid tank; 730. Third waste liquid tank; 800. Second switching valve; 900. Branch pipe; 910. First pipe; 920. Second pipe. Detailed Implementation

[0024] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and not intended to limit it. Furthermore, it should be noted that, for ease of description, the accompanying drawings show only the parts relevant to the present invention, and not all of the structures.

[0025] In the description of this invention, unless otherwise explicitly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0026] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0027] In the description of this embodiment, the terms "upper," "lower," "right," etc., refer to the orientation or positional relationship shown in the accompanying drawings. They are used only for ease of description and simplification of operation, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the present invention. In addition, the terms "first" and "second" are used only for distinction in description and have no special meaning.

[0028] This invention provides a reagent recovery fluid system that can be used for nucleic acid synthesis reactions and to collect reagent waste liquid generated during the nucleic acid synthesis reaction. Of course, in addition to nucleic acid synthesis reactions, this reagent recovery fluid system can also be used in other devices that require synthesis reactions and waste liquid collection.

[0029] like Figure 1 As shown, the reagent recovery fluid system includes a reagent supply component 10, a synthesis component 20, and a recovery component 30 connected in sequence. The reagent supply component 10 includes a reagent source 100, a liquid supply pipeline 200, and a reagent drive pump 300. The synthesis component 20 includes a synthesis device 400 and a waste discharge pipeline 500. The recovery component 30 includes a first switching valve 600 and multiple waste liquid recovery containers 700.

[0030] The reagent source 100 provides liquid reagents, which can be reaction reagents for nucleic acid synthesis or cleaning reagents for cleaning the supply line 200. The supply line 200 is connected to the reagent source 100, and a reagent-driven pump 300 is installed on the supply line 200. Driven by the reagent-driven pump 300, the liquid reagents in the reagent source 100 can enter the synthesis assembly 20 through the supply line 200. The synthesis device 400 has a synthesis chamber, which is the site where the biological carrier and liquid reagents undergo a synthesis reaction. Optionally, the synthesis device 400 is a synthesis column. The other end of the supply line 200, away from the reagent source 100, is connected to the inlet of the synthesis chamber, so that the liquid reagents provided by the reagent source 100 can enter the synthesis chamber through the supply line 200. One end of the waste discharge pipe 500 is connected to the waste discharge port of the synthesis chamber, and the other end is connected to the liquid inlet of the first switching valve 600. Multiple waste liquid recovery containers 700 are connected one-to-one to multiple liquid outlets of the first switching valve 600. Optionally, the first switching valve 600 is a rotary valve. The reagent waste liquid generated after the biological carrier reacts with the liquid reagent can be discharged through the waste discharge port of the synthesis chamber, and the reagent waste liquid can be collected in one of the waste liquid recovery containers 700 through the first switching valve 600. When the reagent recovery fluid system detects that the volume in the waste liquid recovery container 700 has reached a predetermined capacity, it controls the first switching valve 600 to switch the waste discharge flow route within the first switching valve 600 from the current waste liquid recovery container 700 to an empty waste liquid recovery container 700, continuing to collect waste liquid using the empty waste liquid recovery container 700. The entire switching process of the first switching valve 600 takes only a few seconds, which is relatively short. Optionally, the predetermined capacity is approximately equal to the capacity of the waste liquid recovery container 700. In some embodiments, the predetermined capacity is less than the capacity of the waste liquid recovery container 700.

[0031] In some embodiments, the reagent-driven pump 300 pumps a fixed amount of reagent per pumping operation when pumping liquid reagents, and the single-pump reagent volume of the reagent-driven pump 300 is known. During the operation of this reagent recovery fluid system, since the reagent-driven pump 300 pumps a fixed amount of reagent per pumping operation, the reagent recovery fluid system can obtain the total amount of reagent pumped based on the number of pumping operations of the reagent-driven pump 300. Furthermore, since the capacity of the waste liquid recovery container 700 is fixed and known, by comparing the total amount of reagent with the capacity of the waste liquid recovery container 700, it can be determined whether the waste liquid recovery container 700 in use has reached a predetermined capacity. After it is determined that the waste liquid recovery container 700 in use has reached a predetermined capacity, the system can control the first switching valve 600 to switch the waste discharge route within the first switching valve 600 from the waste liquid recovery container 700 that has reached its predetermined capacity to an empty waste liquid recovery container 700.

[0032] Compared to existing technologies that require disassembling the waste liquid recovery container once it reaches a predetermined capacity, emptying the waste liquid, and then reassembling it to continue collecting waste liquid, which not only necessitates a prolonged pause in the synthesis reaction within the synthesis component but also requires constant manual monitoring of whether the waste liquid recovery container has reached its predetermined capacity, the reagent recovery fluid system provided by this invention can not only monitor the amount of reagent waste liquid in real time but also automatically switch to an empty waste liquid recovery container 700 when one waste liquid recovery container 700 reaches its predetermined capacity. The switching time is short, does not affect the synthesis reaction within the synthesis component 20, and helps improve the system's working efficiency and experimental success rate. It also avoids the risk of spillage from the waste liquid recovery container 700 to the laboratory and experimental personnel, thus ensuring high safety.

[0033] In addition to monitoring the number of pumping operations of the reagent-driven pump 300, the operating time of the reagent-driven pump 300 can also be monitored. Since the time for the reagent-driven pump 300 to perform one reagent pumping operation is fixed, the number of pumping operations of the reagent-driven pump 300 can be obtained by monitoring the operating time of the reagent-driven pump 300.

[0034] In some embodiments, the liquid supply line 200 includes a manifold line 210 and at least two branch lines 220. One end of each of the at least two branch lines 220 is connected to one end of the manifold line 210, and the other end of the manifold line 210 is connected to the inlet of the synthesis chamber. Optionally, the at least two branch lines 220 are connected to the manifold line 210 via a multi-port connector. Correspondingly, there are multiple reagent sources 100, and the other ends of the at least two branch lines 220 are respectively connected to the multiple reagent sources 100 one by one. Each branch line 220 is equipped with a reagent drive pump 300.

[0035] It should be noted that the operation of at least one of the multiple reagent-driven pumps 300, by activating the reagent-driven pumps 300 on different branch lines 220, can pump liquid reagents from different reagent sources 100 into the manifold 210, and then into the synthesis chamber of the synthesis device 400 through the manifold 210. When it is necessary to premix different reagents before they enter the synthesis device 400, multiple reagent-driven pumps 300 can be operated simultaneously, so that the liquid reagents from different reagent sources 100 are mixed in the manifold 210 before entering the synthesis chamber of the synthesis device 400. The design of the liquid supply line 200 and the reagent-driven pumps 300 can be adapted to various application scenarios.

[0036] Each reagent source 100 may include one reagent container or multiple reagent containers; the liquid reagents in each reagent container may be the same or different; the liquid reagents may be reaction reagents or cleaning reagents. If the reagent source 100 includes multiple reagent containers, then different reagent containers need to be connected to corresponding branch pipes 220 through supply pipes. The supply pipes are equipped with rotary valves for reagent switching, and the connection between different reagent containers and branch pipes 220 is achieved by controlling the rotary valves.

[0037] In one specific embodiment, continue to refer to Figure 1 As shown, there are two branch pipes 220, which are connected by a T-joint 230; there are also two reagent sources 100 and two reagent drive pumps 300. For ease of description, the two reagent sources 100 are defined as the first reagent source 110 and the second reagent source 120, the two reagent drive pumps 300 are defined as the first drive pump 310 and the second drive pump 320, and the two branch pipes 220 are defined as the first branch pipe 221 and the second branch pipe 222. In this embodiment, there are three operating modes: First, the first driving pump 310 is operational, and the second driving pump 320 is not operational. The liquid reagent in the first reagent source 110, driven by the first driving pump 310, enters the confluence pipe 210 through the first branch pipe 221, and then enters the synthesis chamber of the synthesis device 400 to participate in the reaction. Second, the second driving pump 320 is operational, and the first driving pump 310 is not operational. The liquid reagent in the second reagent source 120, driven by the second driving pump 320, enters the confluence pipe 210 through the second branch pipe 222, and then enters the synthesis chamber of the synthesis device 400 to participate in the reaction. In the first method, the liquid reagent in the reagent source 110 enters the synthesis chamber of the synthesis device 400 to participate in the reaction; in the second method, the first driving pump 310 and the second driving pump 320 work simultaneously. The liquid reagent in the first reagent source 110 passes through the first branch pipe 221 under the drive of the first driving pump 310, and the liquid reagent in the second reagent source 120 passes through the second branch pipe 222 under the drive of the second driving pump 320. The liquid reagent in the first branch pipe 221 and the liquid reagent in the second branch pipe 222 flow into the confluence pipe 210 and enter the synthesis chamber of the synthesis device 400 to participate in the reaction.

[0038] It should be noted that if more than one reagent drive pump 300 is working, the amount of liquid reagent flowing through all the working reagent drive pumps 300 needs to be accumulated and then compared with the volume of the waste liquid recovery container 700. When the accumulated amount is basically equal to the volume of the waste liquid recovery container 700, the first switching valve 600 can be switched so that the waste discharge route is switched from the waste liquid recovery container 700 that has reached the predetermined capacity to the empty waste liquid recovery container 700.

[0039] In some embodiments, the reagent-driven pump 300 is a high-pressure constant-flow pump. Optionally, the high-pressure constant-flow pump is a pump capable of withstanding 5 MPa with minimal pressure pulsation.

[0040] In some parallel embodiments, the reagent-driven pump 300 is an injection dosage pump. Of course, the reagent-driven pump 300 is not limited to a high-pressure constant flow pump or an injection dosage pump, as long as it can pump reagent liquid and can count the amount of reagent liquid flowing through it.

[0041] Continue to refer to Figure 1 As shown, to improve experimental efficiency, multiple synthesis devices 400 are provided, each forming a synthesis chamber. Different synthesis chambers can be used for different experimental steps. In a specific embodiment, there are three synthesis devices 400, which are divided into a first synthesis column 410, a second synthesis column 420, and a third synthesis column 430. The first synthesis column 410, the second synthesis column 420, and the third synthesis column 430 provide a synthesis environment for three different sequences, thereby accelerating the experimental speed of the synthesis reaction.

[0042] To allow liquid reagents to be injected into different synthesis chambers, the synthesis assembly 20 also includes a second switching valve 800. The second switching valve 800 has a main inlet (shown as the IN port in the figure), a main outlet (shown as the OUT port in the figure), and multiple branch flow paths. The main inlet is connected to the other end of the liquid supply line 200, and the main outlet is connected to the waste discharge line 500. The inlets of the multiple branch flow paths can selectively connect to the main inlet, and the outlets of the multiple branch flow paths can selectively connect to the main outlet. Multiple synthesis devices 400 are connected to the multiple branch flow paths one-to-one through multiple branch pipes 900. Optionally, the second switching valve 800 is a rotary valve.

[0043] Optionally, the branch flow path includes a first flow path and a second flow path. One end of the first flow path is a flow path inlet, and the other end forms a first communication port on the valve body of the second switching valve 800. One end of the second flow path is a flow path outlet, and the other end forms a second communication port on the valve body of the second switching valve 800. The branch pipe 900 includes a first pipe 910 and a second pipe 920. The first pipe 910 connects the inlet of the synthesis chamber and the first communication port, and the second pipe 920 connects the waste outlet of the synthesis chamber and the second communication port.

[0044] When the three synthesizing devices 400 include a first synthesizing pillar 410, a second synthesizing pillar 420, and a third synthesizing pillar 430, each of the first and second flow paths has three paths, forming three first connection ports (ports 1, 2, and 3 in the figure) and three second connection ports (ports A, B, and C in the figure); the first tube 910 and the second tube 920 also each have three paths, wherein the three first flow paths are IN port → port 1, IN port → port 2, and IN port → port 3, and the three second flow paths are A port → OUT port, B port → OUT port, and C port → OUT port. Correspondingly, each of the first tube 910 and the second tube 920 has three paths.

[0045] Continue to refer to Figure 1 As shown, in some embodiments, a flow-limiting valve 510 is provided on the waste discharge pipeline 500. The flow-limiting valve 510 ensures that the liquid reagent passes through at a uniform speed by limiting the valve's opening pressure, thereby guaranteeing sufficient reaction between the liquid reagent and the biological carrier in the synthesis device 400. Specifically, the flow-limiting valve 510 is located upstream of the first switching valve 600.

[0046] In some embodiments, continue to refer to Figure 1 As shown, the first switching valve 600 has eight third connection ports on its valve port, designated as third connection port 1, third connection port 2, third connection port 3, third connection port 4, third connection port 5, third connection port 6, third connection port 7, and third connection port 8. Each third connection port can be connected to a waste liquid recovery container 700, and the number of waste liquid recovery containers 700 can be selected according to requirements. In some embodiments, there are eight waste liquid recovery containers 700, each connected to one of the eight third connection ports via eight connecting pipes. Of course, the number of waste liquid recovery containers 700 can be adjusted according to requirements and is not limited to eight. Optionally, the volume of the waste liquid recovery container 700 is 30L.

[0047] In one specific embodiment, continue to refer to Figure 1 As shown, the waste liquid recovery container 700 is a waste liquid tank. There are three waste liquid tanks: the first waste liquid tank 710, the second waste liquid tank 720, and the third waste liquid tank 730. The first waste liquid tank 710 is connected to the third connection port No. 6 on the first switching valve 600, the second waste liquid tank 720 is connected to the third connection port No. 7 on the first switching valve 600, and the third waste liquid tank 730 is connected to the third connection port No. 8 on the first switching valve 600.

[0048] In use, the first switching valve 600 can be controlled to sequentially connect to the third connection port 6, the third connection port 7, and the third connection port 8, thereby enabling the sequential use of the first waste liquid tank 710, the second waste liquid tank 720, and the third waste liquid tank 730. Specifically, when the first switching valve 600 is turned to the third connection port 6, the reagent waste liquid is discharged to the first waste liquid tank 710; when the first switching valve 600 is turned to the third connection port 7, the reagent waste liquid is discharged to the second waste liquid tank 720; and when the first switching valve 600 is turned to the third connection port 8, the reagent waste liquid is discharged to the third waste liquid tank 730.

[0049] In this embodiment, the reagent recovery fluid system also includes a control mechanism, which can be a centralized or distributed controller. For example, the controller can be a single microcontroller or a combination of multiple distributed microcontrollers. The microcontroller can run a control program to control the reagent drive pump 300, the first switching valve 600, the second switching valve 800, etc., to achieve their respective functions.

[0050] The following description uses a reagent recovery fluid system with three synthesis devices 400 and three waste liquid recovery containers 700 as an example to illustrate how to control the first switching valve 600 and the second switching valve 800:

[0051] First, the three sequences are synthesized in the synthesis cavities of the three synthesis devices 400 respectively:

[0052] 1. Sequence synthesis is performed in the first synthesis column 410: The second switching valve 800 switches the internal flow channel of the valve body so that the IN port is connected to port 1 and the A port is connected to the OUT port; the liquid reagent enters the second switching valve 800 through the IN port and flows out from port 1; the liquid reagent enters the first synthesis column 410 through the first tube 910. After the experiment is completed, the reagent waste liquid in the first synthesis column 410 flows out of the first synthesis column 410 through the second tube 920; the reagent waste liquid in the second tube 920 flows back to the second switching valve 800 through the A port and flows into the waste discharge pipeline 500 through the OUT port. This process completes the synthesis of the first sequence in the first synthesis column 410.

[0053] 2. Sequence synthesis in the second synthesis column 420: The second switching valve 800 switches the internal flow channel of the valve body, so that the IN port is connected to port 2 and the B port is connected to the OUT port; the liquid reagent enters the second switching valve 800 through the IN port, and the liquid reagent in the second switching valve 800 flows out from port 2; the liquid reagent enters the second synthesis column 420 through the first tube 910. After the experiment is completed, the reagent waste liquid in the second synthesis column 420 flows out of the second synthesis column 420 through the second tube 920; the reagent waste liquid in the second tube 920 flows back to the second switching valve 800 through the B port, and flows into the waste discharge pipeline 500 through the OUT port. This process completes the synthesis of the second sequence in the second synthesis column 420;

[0054] 3. Sequence synthesis in the third synthesis column 430: The second switching valve 800 switches the internal flow channel of the valve body so that the IN port is connected to port 3 and the C port is connected to the OUT port; the liquid reagent enters the second switching valve 800 through the IN port and flows out from port 3; the liquid reagent enters the third synthesis column 430 through the first tube 910. After the experiment is completed, the reagent waste liquid in the third synthesis column 430 flows out of the third synthesis column 430 through the second tube 920; the reagent waste liquid in the second tube 920 flows back to the second switching valve 800 through the C port and flows into the waste discharge pipeline 500 through the OUT port. This process completes the synthesis of the third sequence in the third synthesis column 430.

[0055] Assuming that, in the above process, after the first sequence is synthesized in the first synthesis column 410, the control mechanism has recorded that the amount of reagent waste liquid stored in the first waste liquid tank 710 (capacity of 30L) is 25L, and it is expected that the synthesis of the second sequence in the second synthesis column 420 will generate approximately 10L of reagent waste liquid, then during the synthesis of the second sequence in the second synthesis column 420, if it is monitored that the second synthesis column 420 has cumulatively discharged 4.5L of reagent waste liquid into the first waste liquid tank 710 (at this time, the first waste liquid tank 710 has a cumulative amount of 29.5L of reagent waste liquid), the control mechanism sends a signal to switch the waste liquid recovery container 700. After the current reaction step is completed in the second synthesis column 420, it pauses for 5 seconds. During the pause, the first switching valve 600 receives the signal and switches to the third connection port 7, so that the second synthesis column 420 is connected to the second waste liquid tank 720. After that, the synthesis of the second sequence continues in the second synthesis column 420, and reagent waste liquid is discharged into the second waste liquid tank 720. The switching process for other waste liquid recovery containers 700 is similar, and will not be listed here.

[0056] In summary, this reagent recovery fluid system ensures that when a waste liquid recovery container 700 is nearly full, the first switching valve 600 automatically switches to connect to the next waste liquid recovery container 700. This not only eliminates the need for manual disassembly and assembly of the waste liquid recovery container 700 during the synthesis process, resolving the need for prolonged instrument interruptions, but also prevents the risk of reagent waste overflow from the waste liquid recovery container 700, thus avoiding damage to the laboratory and personal safety. Furthermore, this reagent recovery fluid system has a simple structure, low cost, high reliability, and its artificial intelligence monitoring eliminates the risk of malfunction, thereby improving production efficiency.

[0057] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the implementation of the present invention. Those skilled in the art will be able to make various obvious changes, readjustments, and substitutions without departing from the scope of protection of the present invention. It is neither necessary nor possible to exhaustively describe all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the claims of the present invention.

Claims

1. A reagent recovery fluid system, characterized in that, include: A reagent supply assembly (10) includes a reagent source (100), a liquid supply line (200), and a reagent drive pump (300). One end of the liquid supply line (200) is connected to the reagent source (100), and the reagent drive pump (300) is mounted on the liquid supply line (200). A synthesis assembly (20) includes a synthesis device (400) and a waste discharge pipe (500). The synthesis device (400) has a synthesis chamber. The other end of the liquid supply pipe (200) is connected to the inlet of the synthesis chamber, and one end of the waste discharge pipe (500) is connected to the waste discharge port of the synthesis chamber. The recycling component (30) includes a first switching valve (600) and a plurality of waste liquid recycling containers (700). The other end of the waste discharge pipeline (500) is connected to the inlet of the first switching valve (600), and the plurality of waste liquid recycling containers (700) are connected one-to-one to the plurality of outlets of the first switching valve (600). The reagent recovery fluid system detects that the volume of the waste liquid recovery container (700) has reached a predetermined volume and controls the first switching valve (600) to operate, so that the waste discharge flow route in the first switching valve (600) is switched from the current waste liquid recovery container (700) to the empty waste liquid recovery container (700).

2. The reagent recovery fluid system according to claim 1, characterized in that, The reagent driving pump (300) pumps a fixed amount of reagent per pumping. The reagent recovery fluid system can obtain the total amount of reagent pumped based on the number of pumping cycles of the reagent driving pump (300), and can control the operation of the first switching valve (600) based on the total amount of reagent and the capacity of the waste liquid recovery container (700).

3. The reagent recovery fluid system according to claim 1, characterized in that, The liquid supply line (200) includes a confluence line (210) and at least two branch lines (220), one end of each of the at least two branch lines (220) is connected to one end of the confluence line (210), and the other end of the confluence line (210) is connected to the inlet of the synthesis chamber; The reagent source (100) is provided with at least two, and the other end of the at least two branch pipes (220) are respectively connected to the at least two reagent sources (100) in a one-to-one correspondence. Each branch pipe (220) is provided with a reagent drive pump (300).

4. The reagent recovery fluid system according to claim 3, characterized in that, At least one of the plurality of said reagent drive pumps (300) is operational, and different said reagent sources (100) contain the same or different reagents, said reagents including reaction reagents and cleaning reagents.

5. The reagent recovery fluid system according to claim 3, characterized in that, At least two of the branch lines (220) are connected to the confluence line (210) via a multi-port connector.

6. The reagent recovery fluid system according to claim 1, characterized in that, The reagent drive pump (300) is a high-pressure constant flow pump; Alternatively, the reagent-driven pump (300) may be an injection dosing pump.

7. The reagent recovery fluid system according to claim 1, characterized in that, The synthesis device (400) is provided with multiple components; The synthesis component (20) further includes a second switching valve (800), which has a main inlet, a main outlet and multiple branch flow paths. The main inlet is connected to the other end of the liquid supply pipeline (200), and the main outlet is connected to the waste discharge pipeline (500). The flow path inlets of the multiple branch flow paths can be selectively connected to the main inlet, and the flow path outlets of the multiple branch flow paths can be selectively connected to the main outlet. The multiple synthesis devices (400) are respectively connected to the multiple branch flow paths one by one through multiple branch pipes (900).

8. The reagent recovery fluid system according to claim 7, characterized in that, The branch flow path includes a first flow path and a second flow path. One end of the first flow path is the flow path inlet, and the other end forms a first communication port on the valve body of the second switching valve (800). One end of the second flow path is the flow path outlet, and the other end forms a second communication port on the valve body of the second switching valve (800). The branch pipe (900) includes a first pipe (910) and a second pipe (920), the first pipe (910) being connected between the inlet of the synthesis chamber and the first connecting port, and the second pipe (920) being connected between the waste outlet of the synthesis chamber and the second connecting port.

9. The reagent recovery fluid system according to claim 1, characterized in that, The waste discharge pipeline (500) is equipped with a flow limiting valve (510).

10. The reagent recovery fluid system according to any one of claims 1-9, characterized in that, The reagent source (100) includes multiple reagent containers, each containing the same or different reagents.

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