Sample collection device for detecting coccidian oocysts and sample detection method

Abstract

The invention discloses a sample collecting device for detecting coccidian oocysts and a sample detecting method.The sample collecting device comprises a sampling rod, a collecting pipe, a rotating bin and a transferring assembly, at least one collecting groove is formed in the peripheral wall of the lower end of the sampling rod, a recycling groove is formed in the upper portion of the collecting groove, and a closed side plate capable of moving up and down is arranged in the recycling groove; a storage channel is formed in the position, corresponding to the collecting groove, of the lower end of the sampling rod, the rotating bin comprises an upper rotating ring and a lower rotating ring, the rotating rings can rotate with the center axis of the sampling rod as a rotating shaft, a plurality of containing cavities are formed in the rotating rings in a circumferential array mode, and a communicating window is formed in the position, corresponding to the rotating bin, of the side wall of the sampling rod; the containing cavity is provided with an open channel extending towards the side wall of the sampling rod, a gap is formed between the upper portion and the lower portion of the wheel rotating ring, a telescopic chuck is installed outside the sampling rod, and the telescopic chuck can extend to penetrate through the gap between the wheel rotating rings and extend into the containing cavity so as to grab the collecting pipe to penetrate through the communicating window from the containing cavity to move into the sampling rod.

Description

Technical Field

[0001] This invention relates to the field of biological detection technology, and in particular to a sample collection device and sample detection method for detecting coccidia oocysts. Background Technology

[0002] Coccidiosis surveillance is a diagnostic and preventative measure for coccidiosis, a parasitic disease caused by one or more coccidia. It is common in poultry and livestock, especially in intensive farming environments. The surveillance and control of coccidiosis is crucial for reducing economic losses in the livestock industry.

[0003] Porcine coccidia destroys intestinal villi, causing indigestion and infections by other intestinal pathogens, such as clostridial enteritis and Escherichia coli infection. Current methods for coccidia detection include: 1. Direct microscopic examination: This is a simple and rapid method, but because coccidia oocysts are (20-23)*(17-19) μm in size and lack micropores, direct microscopic examination of fecal smears has limited value, resulting in a low detection rate and difficulty in identifying oocysts; 2. Salt-sugar solution flotation method: (100 mL saturated saline solution + 50 g sugar, density 1.226) This method has a high detection rate and is the most commonly used method. However, the presence of fat particles can interfere with oocyst detection, requiring some experience to achieve a fast and accurate detection rate, but it is relatively the easiest method to master; 3. Teleman method: Also known as centrifugation, this is a more effective method for oocyst detection because ether can remove fatty substances from feces. However, it is also quite cumbersome in actual operation; 4. PCR method: If it is to be determined whether it is Isospora infection, sporulation is required, which takes a long time. PCR detection method can detect whether it is infected with Isospora suis more quickly, but the steps are cumbersome and the cost is high; 5. Indirect ELISA method: This experiment uses highly purified fresh and impurity-free sporulated oocysts of Isospora suis to prepare oocyst homogenate as coating antigen to establish an indirect ELISA method. It overcomes the disadvantages of direct microscopic examination, which is time-consuming, laborious and has a low detection rate, but the cost is high and the experimental conditions are more demanding. It can only be used as a method for establishing Isospora suis.

[0004] Regardless of the method used to test the samples, technicians must first collect and process the fecal samples. Publication number CN214907288U discloses a fecal sampling device for animal husbandry and veterinary use, including a sampling cylinder and a sampling rod installed inside the sampling cylinder. A retaining ring is provided inside the sampling cylinder, and the sampling rod passes through the retaining ring. A first baffle on the sampling rod abuts against the front side of the retaining ring, and a second baffle is installed at the rear end of the sampling rod. A telescopic spring is fitted on the sampling rod, with both ends abutting against the retaining ring and the second baffle, respectively. Multiple alternating scrapers are provided on the outer wall of the front half of the sampling rod. A push rod is connected to the rear end of the sampling rod, with its end extending out of the sampling cylinder and equipped with a push plate. Holding the push plate allows the push rod to be pushed forward, causing the sampling rod to extend out of the sampling cylinder and into the animal's anus. The scrapers in the front half of the sampling rod can scrape off the feces. Releasing the push plate detaches the sampling rod from the animal's anus, completing the fecal sampling. This fecal sampling process is relatively convenient and quick.

[0005] The device only has a sampling function. After the user uses the device to remove the feces, the user still needs to manually fill the removed feces sample into the sample transfer container. During this process, the sample will not only be exposed to the outside and may be contaminated, causing detection errors, but the feces itself will also be exposed to the outside environment during the transfer process, causing environmental pollution and potentially causing discomfort to the transfer operators. Summary of the Invention

[0006] To address the problems mentioned in the background section, this invention provides a sample collection device and a sample detection method for detecting coccidia oocysts.

[0007] To achieve the above objectives, the present invention adopts the following technical solution: A sample collection device for detecting coccidia oocysts includes a sampling rod, a collection tube, a rotating chamber, and a transfer assembly. The sampling rod has at least one collection groove on its lower peripheral wall, and a recovery groove on its upper part. A closed side plate that can move up and down is provided in the recovery groove. A storage channel is provided at the lower end of the sampling rod corresponding to the collection groove. A bottom sealing block is provided in the storage channel. A barrier block that can move up and down is provided between the storage channel and the collection groove. The bottom sealing block can be moved by a translation drive rod and can be detachably connected to the closed side plate. The transfer assembly can move in a transfer chamber that is provided above the collection groove and communicates with it, so as to insert the open end of the transfer tube at the bottom into the communication channel between the collection groove and the transfer chamber. The rotating chamber includes two rotating rings, one above the other, which can rotate around the central axis of the sampling rod. The rotating rings have multiple placement cavities arranged in a circular array. The side wall of the sampling rod has a connecting window at a position corresponding to the rotating chamber. The placement cavity has a channel that extends and opens toward the side wall of the sampling rod. There is a gap between the upper and lower rotating rings. A telescopic clamp is installed on the outside of the sampling rod. The telescopic clamp can extend and pass through the gap between the rotating rings and probe into the placement cavity to grab the collection tube and move it from the placement cavity through the connecting window into the sampling rod.

[0008] Preferably, when the closed side plate extends downward into the collection tank, there is a gap between it and the inner side wall of the collection tank, so that after the feces fill the collection tank, the closed side plate moved into the collection tank can cut off the feces in the collection tank from the outside, thereby allowing the feces remaining in the collection tank to be sampled.

[0009] Preferably, the height difference between the barrier block and the storage channel is exactly equal to the thickness of the bottom sealing block, so that when the barrier block is lifted or lowered to the top or bottom of the storage channel by the hoisting motor, the bottom sealing block can just pass through the gap between the barrier block and the bottom or top of the storage channel and enter the collection tank. The bottom sealing block includes a main block and a connector facing the collection tank. The horizontal cross-section of the main block can just fill the gap between the closed side plate and the collection tank. The bottom of the closed side plate has a connector that allows the connector to be inserted. The connector can be detachably connected to the connector.

[0010] Preferably, the translation drive rod is installed at the upper and lower ends of the storage channel. An electromagnetic docking block is installed at the end of the translation drive rod. The side of the main block facing the translation drive rod has a groove that can just dock with the electromagnetic docking block. A permanent magnet is installed in the groove of the main block. After the electromagnetic docking block is energized and generates strong magnetism, it can connect with the bottom sealing block with a sufficiently strong magnetic attraction force, so that the translation drive rod can drive the bottom sealing block to detach from the closed side plate that is matched and connected, or overcome the resistance to push the bottom sealing block and insert the docking connector into the docking interface so that the bottom sealing block and the closed side plate are matched and connected.

[0011] Preferably, the transfer assembly includes a transfer cylinder, a telescopic frame, and a vertical transfer rod. The vertical transfer rod is controlled by a controller to be hydraulically telescopic. The telescopic frame is installed on the outer periphery of the sub-control head at the lower end of the vertical transfer rod. The transfer cylinder is installed at the end of the telescopic frame away from the sub-control head. The transfer cylinder is hollow inside and open at the bottom. A sealing cover is installed at the bottom of the transfer cylinder. The sealing cover is connected to the bottom edge of the transfer cylinder by an electric hinge to control the opening and closing of the bottom end of the transfer cylinder.

[0012] Preferably, the transfer chamber has a transfer channel extending to the location of the wheel chamber. An end motor is installed at the top of the transfer cylinder. The end motor is used to drive the screw inside the transfer cylinder to rotate axially, thereby driving the discharge push block inside the transfer cylinder to move up and down. The outer peripheral wall of the discharge push block is in close contact with the inner wall of the transfer cylinder, so that when the discharge push block moves downward, it can push out all the fecal samples in the transfer cylinder from the opening at the bottom of the transfer cylinder.

[0013] Preferably, the rotating ring is detachably fitted with a shielding plate for closing the opening of the placement cavity, so that the user can keep the collection tube inside the placement cavity by installing the shielding plate after the collection tube is placed in the placement cavity.

[0014] A sample detection method for coccidia oocysts is also provided, including the following steps: S1: Insert the end of the sampling rod with the collection groove into a pile of fresh livestock manure or the rectum of a livestock to obtain a manure sample; S2: Remove the collection tube containing the fecal sample from the rotating chamber, add saturated urea solution to the collection tube so that the ratio of feces to saturated urea solution in the collection tube is 1:10-1:20, and then stir the feces and saturated urea solution thoroughly to make the feces completely dispersed in the saturated urea solution and form a uniform suspension. S3: Place the collection tube containing the mixed suspension into a centrifuge, set the centrifugation speed to 1500-2000 rpm, and the centrifugation time to 5-10 minutes. After centrifugation, the coccidia oocysts will float to the surface of the liquid. S4: Carefully aspirate a layer of liquid from the surface using a pipette or pipette and transfer it to a glass slide. Then, add an appropriate amount of sealing medium to the glass slide and gently cover it with a coverslip to avoid creating air bubbles. S5: Place the prepared slide under a microscope for observation to detect information such as the type and number of coccidia.

[0015] Compared with the prior art, the beneficial effects of the present invention are: 1. This invention uses a method of mixing saturated urea with fecal samples to process the samples. The specific gravity of coccidia oocysts is smaller than that of saturated urea solution. After centrifugation and other operations, the oocysts will float to the surface of the liquid. At the same time, urea accelerates protein sedimentation and reduces interfering foreign matter such as fat particles, making it easier to collect and observe. This invention is a method for enriching oocysts based on the physical characteristics and density differences of coccidia, which can increase the sensitivity of detection and make it easier to find coccidia oocysts.

[0016] 2. The sampling device used in this invention can simultaneously sample fresh manure piles and rectums of livestock, enabling sampling in multiple scenarios and improving applicability. Furthermore, the invention can be equipped with multiple collection slots, allowing for multi-point sampling in a single sampling operation, resulting in more random sample acquisition and reduced detection errors.

[0017] 3. The entire process of sampling and transferring fecal samples to the collection tube can be automated within the sampling rod through the coordinated work of the rotating chamber, transfer components, and gripping components. This avoids environmental pollution caused by the fecal samples being exposed to the outside world during the transfer process, and the entire transfer process is conducted without human intervention, so it will not cause discomfort to the user. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the sample collection device for detecting coccidia oocysts according to the present invention; Figure 2 This is a schematic diagram of the bottom structure of the sampling rod of a sample collection device for detecting coccidia oocysts according to the present invention; Figure 3 for Figure 2 Enlarged view of point A in the middle; Figure 4 for Figure 2 Enlarged view of point B in the middle; Figure 5 This is a schematic diagram of the cooperation structure between the rotating chamber and the sampling rod described in this invention; Figure 6 This is a schematic diagram of the cooperative structure of the rotating bin and the gripping and moving component described in this invention.

[0020] In the diagram: 1. Sampling rod; 101. Collection tank; 102. Recycling tank; 103. Storage channel; 104. Connecting interface; 105. Transfer chamber; 106. Transfer channel; 107. Anti-detachment slide rail; 108. Connecting window; 11. Enclosed side plate; 12. Lifting motor; 13. Interactive device; 14. Controller; 15. Bottom sealing block; 151. Main block; 152. Connecting interface; 16. Barrier block; 17. Lifting motor; 18. 19. Translation drive rod; 2. Electromagnetic docking block; 3. Collection pipe; 4. Rotary bin; 5. Guide ring rail; 6. Placement cavity; 7. Rotary ring; 8. Drive frame rod; 9. Drive device; 10. Shielding plate; 11. Transfer assembly; 12. Transfer cylinder; 23. Telescopic frame; 34. Vertical transfer rod; 55. Control head; 66. Sealing cover; 77. End motor; 88. Discharge push block; 99. Grab and transfer assembly; 100. Fixing block; 11. Telescopic clamp. Detailed Implementation

[0021] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Example 1

[0022] Reference Figure 1-6 A sample collection device for detecting coccidia oocysts includes a sampling rod 1, a collection tube 2, a rotating chamber 3, and a transfer assembly 4. The rotating chamber 3 is installed on the upper part of the sampling rod 1, the collection tube 2 is removably installed in the rotating chamber 3, and the transfer assembly 4 is installed inside the sampling rod 1.

[0023] The sampling rod 1 has a rounded probe at its lower end, and at least one collection groove 101 is provided on the outer peripheral wall of the probe. A recovery groove 102 is provided above the collection groove 101, and a closed side plate 11 is provided inside the recovery groove 102. A lifting motor 12 is installed above the recovery groove 102. The lifting motor 12 is connected to the closed side plate 11 via a hydraulic telescopic rod. The lifting motor 12 can drive the closed side plate 11 downwards into the recovery groove 102 and into the collection groove 101 by controlling the extension and retraction of the hydraulic telescopic rod, or move upwards to retract into the recovery groove 102, with the closed side plate 11 extending downwards into the collection groove. When sampling, the closed side plate 11 is spaced apart from the inner wall of the collection tank 101. Before sampling, the closed side plate 11 is moved into the collection tank 102. After the user inserts the rounded probe of the sampling rod 1 into the animal's rectum or manure pile, the manure will fill the collection tank 101. At this time, the user inputs a command to the interactive device 13 installed on the outer periphery of the sampling rod 1, so that the controller 14 controls the lifting motor 12 to drive the closed side plate 11 to extend downward into the collection tank 101 after receiving the command. At this time, the closed side plate 11 cuts off the manure in the collection tank 101 from the outside, and some manure is left in the collection tank 101 as a sample.

[0024] A storage channel 103 is provided at the lower end of the sampling rod 1, corresponding to the position of the collection tank 101. A bottom sealing block 15 is provided in the storage channel 103. A barrier block 16 that can move up and down is provided between the storage channel 103 and the collection tank 101. The height difference between the barrier block 16 and the storage channel 103 is exactly equal to the thickness of the bottom sealing block 15. When the barrier block 16 is lifted or lowered to the top or bottom of the storage channel 103 by the hoisting motor 17, the bottom sealing block 15 can just pass through the gap between the barrier block 16 and the bottom or top of the storage channel 103 and enter the collection tank 101. When the bottom sealing block 15 is in the gap between the barrier block 16 and the bottom or top of the storage channel 103, it can also completely isolate the storage channel 103 from the collection tank 101 to prevent feces from entering the storage channel 103 and affecting the up and down movement of the bottom sealing block 15 within it.

[0025] The bottom sealing block 15 includes a main block 151 and a connector 152 facing the collection groove 101. The horizontal cross-section of the main block 151 can just fill the gap between the closed side plate 11 and the collection groove 101. The bottom of the closed side plate 11 has a mating interface 104 that allows the connector 152 to be inserted. The connector 152 and the mating interface 104 are detachably connected by a mating structure such as a groove and a spring contact. Each of the upper and lower ends of the storage channel 103 is equipped with a translation drive rod 18. An electromagnetic docking block 19 is installed at the end of the translation drive rod 18. The side of the main block 151 facing the translation drive rod 18 has a groove that can just dock with the electromagnetic docking block 19. A permanent magnet is installed in the groove of the main block 151. After the translation drive rod 18 is extended, the electromagnetic docking block 19 can dock with the groove of the main block 151.

[0026] After the electromagnetic docking block 19 is energized and generates strong magnetism, the translation drive rod 18 with the electromagnetic docking block 19 installed at its end is magnetically connected to the bottom sealing block 15. This allows the extension and retraction of the translation drive rod 18 to drive the bottom sealing block 15 to move horizontally. Since the magnetic connection between the bottom sealing block 15 and the electromagnetic docking block 19 is sufficiently tight, the translation drive rod 18 can drive the bottom sealing block 15 to disengage from the corresponding closed side plate 11, or overcome resistance to push the bottom sealing block 15 and insert the connector 152 into the interface 104, thus connecting the bottom sealing block 15 with the closed side plate 11. The electromagnetic docking block 19 can also be de-energized to release the magnetic connection with the bottom sealing block 15, thereby shortening the translation drive rod 18 and causing the electromagnetic docking block 19 to disengage from the bottom sealing block 15 while keeping the bottom sealing block 15 connected to the closed side plate 11, so that the closed side plate 11 can move vertically within the collection trough 101.

[0027] A transfer chamber 105 communicating with the collection trough 101 is provided above it. The transfer assembly 4 includes a transfer cylinder 41, a telescopic frame 42, and a vertical moving rod 43. The vertical moving rod 43 is controlled by the controller 14 to be hydraulically telescopic. The telescopic frame 42 is installed on the outer periphery of the sub-control head 44 at the lower end of the vertical moving rod 43. The transfer cylinder 41 is installed at the end of the telescopic frame 42 away from the sub-control head 44. The transfer cylinder 41 is hollow inside and open at the bottom. A sealing cover 45 is installed at the bottom of the transfer cylinder 41. The sealing cover 45 is connected to the bottom edge of the transfer cylinder 41 by an electric hinge to control the opening and closing of the bottom of the transfer cylinder 41.

[0028] During fecal sampling, the transfer cylinder 41 is inserted into the communication channel between the collection tank 101 and the transfer chamber 105 with its bottom sealed, thus sealing the upper part of the collection tank 101. After the sealing side plate 11 moves down to block the feces in the collection tank 101 from the outside, the bottom sealing block 15 at the bottom of the storage channel 103 is pushed out by the translation drive rod 18 and connected to the sealing side plate 11. At this time, the fecal sample above the bottom sealing block 15 will be blocked from the feces below. Then, the transfer cylinder 41 is raised a little distance to make room for the sealing cover 45 to unfold. The transfer cylinder 41 is then reconnected to the collection tank 101 after the lower end is closed. Finally, the lifting motor 12 pulls the sealing side plate 11 and the bottom sealing block 15 connected to it to move towards the transfer cylinder 41, thereby squeezing the fecal sample in the collection tank 101 into the transfer cylinder 41. After the fecal sample is squeezed into the transfer cylinder 41, the sealing cover 45 is driven by the electric hinge to keep the lower end of the transfer cylinder 41 closed.

[0029] After the bottom sealing block 15 moves to the top of the collection tank 101 along with the closed side plate 11, the barrier block 16, which was originally lifted by the hoisting motor 17 and pressed against the top surface of the storage channel 103, is lowered, thereby opening a space at the top of the storage channel 103 to allow the bottom sealing block 15 to move into the storage channel 103. At this time, the translation drive rod 18 set at the top of the storage channel 103 extends and magnetically connects the electromagnetic docking block 19 at its end to the bottom sealing block 15, thereby pulling the bottom sealing block 15 away from the closed side plate 11, and moving back into the storage channel 103 as the translation drive rod 18 retracts. The side wall of the storage channel 103 restricts the bottom sealing block 15 to move vertically within it and prevents it from rotating or flipping. After the bottom sealing block 15 moves back into the storage channel 103, the barrier block 16 moves up again, leaving gaps at both ends of the barrier block 16 that prevent the bottom sealing block 15 from moving out, so that the bottom sealing block 15 is stored in the storage channel 103 until the next sampling. Example 2

[0030] Reference Figure 1-6The difference between this embodiment and Embodiment 1 is that the transfer cavity 105 has a transfer channel 106 extending to the location of the rotating chamber 3. After receiving the fecal sample, the transfer cylinder 41 will move into the transfer cavity 105 along with the telescopic frame 42 due to the shortening of the vertical moving rod 43. Then, the control head 44 controls the telescopic frame 42 to shorten, causing the transfer cylinder 41 to move towards the central axis of the sampling rod 1, so that the transfer cylinder 41 can continue to enter the transfer channel 106 as the vertical moving rod 43 shortens. An end motor 46 is installed at the top of the transfer cylinder 41. The end motor 46 is used to drive the screw inside the transfer cylinder 41 to rotate axially, thereby driving the discharge push block 47 set inside the transfer cylinder 41 to move up and down. The outer peripheral wall of the discharge push block 47 is in close contact with the inner wall of the transfer cylinder 41, so that when the discharge push block 47 moves downward, it can push all the fecal sample in the transfer cylinder 41 out from the opening at the bottom of the transfer cylinder 41.

[0031] The rotating chamber 3 includes two rotating rings 31, one above the other. The rotating rings 31 are held in a designated vertical position by a drive frame rod 32 installed outside the sampling rod 1. One end of the drive frame rod 32 is fixedly installed on the sampling rod 1, and the other end is equipped with a drive device 33 that cooperates with the guide ring rail 301 of the rotating ring 31. The guide ring rail 301 is arranged in a ring around the rotating ring 31. The drive device 33 can drive the wheel that is engaged with the guide ring rail 301 to rotate, thereby driving the rotating ring 31 to rotate about the central axis of the sampling rod 1.

[0032] The rotating ring 31 has multiple placement cavities 302 arranged in a circular array. Each placement cavity 302 passes through the rotating ring 31 vertically, allowing the collection tube 2 to be placed inside and rotate around the sampling rod 1 with the rotating ring 31. The upper surface of the upper rotating ring 31 and the lower surface of the lower rotating ring 31 are detachably equipped with shielding plates 34 for sealing the openings of the placement cavities 302. Before sampling, the user can place the collection tube 2 into the placement cavity 302 by removing the shielding plate 34 set on the upper rotating ring 31. After the collection tube 2 is placed into the placement cavity 302, the user can keep the collection tube 2 in the placement cavity 302 by installing the shielding plate 34. After sampling is completed, the user can remove the collection tube 2 from the placement cavity 302 by removing the shielding plate 34 set on the lower rotating ring 31.

[0033] Preferably, the outer peripheral wall of the sampling rod 1 is provided with an anti-detachment slide rail 107, and the edge of the shielding plate 34 near the outer peripheral wall of the sampling rod 1 is provided with a locking block that engages with the anti-detachment slide rail 107. This allows the shielding plate 34 to move vertically along the anti-detachment slide rail 107 after being removed and detached from the rotating ring 31, thus freeing up space for the collection tube 2 to be inserted into and removed from the placement cavity 302, and maintaining a connection with the sampling rod 1 without detachment or loss, which would cause inconvenience.

[0034] A connecting window 108 is provided on the side wall of the sampling rod 1 at a position corresponding to the rotating chamber 3. The placement cavity 302 has a channel extending towards the side wall of the sampling rod 1, so that when the collection tube 2 in the placement cavity 302 moves to a position corresponding to the connecting window 108, it can pass through the connecting window 108 and enter the transfer channel 106.

[0035] A gripping and moving assembly 5 is installed on the outer peripheral wall of the sampling rod 1 at a position corresponding to the connecting window 108. The gripping and moving assembly 5 includes a fixing block 51 fixedly installed on the outer peripheral wall of the sampling rod 1. The side of the fixing block 51 facing the sampling rod 1 has a space that allows the rotating ring 31 to pass through. There is a gap between the upper and lower rotating rings 31. A telescopic clamp 52 is installed on the fixing block 51 at a position corresponding to the gap between the two rotating rings 31. One end of the telescopic clamp 52 is fixedly installed on the fixing block 51, and the other end can extend to pass through the gap between the two rotating rings 31 and enter the placement cavity 302. A mechanical gripper is installed on the end of the telescopic clamp 52 that can enter the placement cavity 302. After the mechanical gripper enters the placement cavity 302, it can grip the collection tube 2, thereby moving the collection tube 2 from the placement cavity 302 through the connecting window 108 into the transfer channel 106.

[0036] After loading the fecal sample, the transfer cylinder 41 moves upward as the vertical moving rod 43 shortens until it is above the rotating chamber 3. When the telescopic clamp 52 grabs the collection tube 2 and moves it below the transfer cylinder 41, the discharge push block 47 can squeeze the fecal sample downward and receive it by the collection tube 2. After receiving the fecal sample, the collection tube 2 is moved back to the placement chamber 302 by the telescopic clamp 52. After the collection tube 2 loaded with the fecal sample moves back to the placement chamber 302, it rotates with the rotating ring 31 so that an adjacent collection tube 2 that has not received a fecal sample moves to the position corresponding to the connecting window 108. After the transfer assembly 4 obtains the next batch of fecal samples and moves the transfer cylinder 41 above the rotating chamber 3, this collection tube 2 that has not received a fecal sample can be moved into the transfer channel 106 by the telescopic clamp 52 to receive the newly obtained fecal sample. In this way, all collection tubes 2 can be loaded with fecal samples according to the above steps. Example 3

[0037] A method for detecting coccidia oocysts, comprising the following steps: S1: Insert one end of the sampling rod 1 with the collection groove 101 into a pile of fresh livestock manure or the rectum of a livestock to obtain a manure sample; S2: Take the collection tube 2 containing the fecal sample out of the rotating chamber 3, add saturated urea solution to the collection tube 2 so that the ratio of feces to saturated urea solution in the collection tube 2 is 1:10-1:20, and then stir the feces and saturated urea solution thoroughly to make the feces completely dispersed in the saturated urea solution to form a uniform suspension. S3: Place the collection tube 2 containing the mixed suspension into the centrifuge, set the centrifugation speed to 1500-2000 rpm, and the centrifugation time to 5-10 minutes. After centrifugation, the coccidia oocysts will float to the surface of the liquid. S4: Carefully aspirate a layer of liquid (containing floating egg sacs) from the surface of the liquid using a pipette or pipette, transfer it to a glass slide, then add an appropriate amount of mounting medium (such as glycerin) to the glass slide, and gently cover it with a coverslip to avoid generating air bubbles; S5: Place the prepared slide under a microscope for observation to detect information such as the type and number of coccidia.

[0038] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0039] In this invention, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," "link," and "fix" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, a direct connection, or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0040] The control method of this invention is automatic control through a controller. The control circuit of the controller can be implemented by simple programming by those skilled in the art. The power supply is also common knowledge in the art. Furthermore, since this invention is mainly used to protect mechanical devices, the control method and circuit connection will not be explained in detail here.

[0041] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A sample collection device for detecting coccidia oocysts, comprising a sampling rod (1), a collection tube (2), a rotating chamber (3), and a transfer assembly (4), characterized in that: The sampling rod (1) has at least one collection groove (101) on its lower peripheral wall. The collection groove (101) has a recycling groove (102) on its upper part. The recycling groove (102) has a closed side plate (11) that can move up and down. The sampling rod (1) has a storage channel (103) at the position corresponding to the collection groove (101) at its lower end. The storage channel (103) has a bottom sealing block (15). The storage channel (103) and the collection groove (101) have a barrier block (16) that can move up and down. The bottom sealing block (15) can be moved by the translation drive rod (18) and can be detachably connected to the closed side plate (11). The transfer assembly (4) can move in the transfer cavity (105) above the collection groove (101) and communicate with it, so as to insert the open end of the transfer cylinder (41) into the communication channel between the collection groove (101) and the transfer cavity (105). The rotating chamber (3) includes two rotating rings (31) on the top and bottom. The rotating rings (31) can rotate around the central axis of the sampling rod (1). The rotating rings (31) have multiple placement cavities (302) arranged in a circular array. The side wall of the sampling rod (1) is provided with a connecting window (108) at a position corresponding to the rotating chamber (3). The placement cavity (302) has a channel extending towards the side wall of the sampling rod (1). There is a gap between the upper and lower rotating rings (31). A telescopic clamp (52) is installed on the outside of the sampling rod (1). The telescopic clamp (52) can extend and pass through the gap between the rotating rings (31) and probe into the placement cavity (302) to grab the collection tube 2 and move it from the placement cavity (302) through the connecting window (108) into the sampling rod (1).

2. The sample collection device for detecting coccidia oocysts according to claim 1, characterized in that: When the closed side plate (11) extends downward into the collection tank (101), it has a gap distance from the inner side wall of the collection tank (101) so that after the feces fill the collection tank (101), the closed side plate (11) moved into the collection tank (101) can cut off the feces in the collection tank (101) from the outside, so that the feces remaining in the collection tank (101) can be sampled and extracted.

3. The sample collection device for detecting coccidia oocysts according to claim 1, characterized in that: The height difference between the barrier block (16) and the storage channel (103) is exactly equal to the thickness of the bottom sealing block (15), so that when the barrier block (16) is lifted or lowered to the top or bottom of the storage channel (103) by the hoisting motor (17), the bottom sealing block (15) can just pass through the gap between the barrier block (16) and the bottom or top of the storage channel (103) and enter the collection tank (101). The bottom sealing block (15) includes a main block (151) and a connector (152) facing the collection tank (101). The horizontal cross section of the main block (151) can just fill the gap between the closed side plate (11) and the collection tank (101). The bottom of the closed side plate (11) has a connector (104) that allows the connector (152) to be inserted. The connector (152) can be detachably connected to the connector (104).

4. The sample collection device for detecting coccidia oocysts according to claim 3, characterized in that: The translation drive rod (18) is installed at the upper and lower ends of the storage channel (103). An electromagnetic docking block (19) is installed at the end of the translation drive rod (18). The main block (151) has a groove on the side facing the translation drive rod (18) that can dock with the electromagnetic docking block (19). A permanent magnet is installed in the groove of the main block (151). After the electromagnetic docking block (19) generates strong magnetism when energized, it can connect with the bottom sealing block (15) with a sufficiently strong magnetic attraction force, so that the translation drive rod (18) can drive the bottom sealing block (15) to disengage from the closed side plate (11) that is matched and connected, or overcome the resistance to push the bottom sealing block (15) and insert the docking connector (152) into the docking interface (104) so ​​that the bottom sealing block (15) and the closed side plate (11) are matched and connected.

5. The sample collection device for detecting coccidia oocysts according to claim 1, characterized in that: The transfer assembly (4) includes a transfer cylinder (41), a telescopic frame (42), and a vertical moving rod (43). The vertical moving rod (43) is controlled by a controller (14) to be hydraulically telescopic. The telescopic frame (42) is installed on the outer periphery of the sub-control head (44) at the lower end of the vertical moving rod (43). The transfer cylinder (41) is installed at the end of the telescopic frame (42) away from the sub-control head (44). The transfer cylinder (41) is hollow inside and open at the bottom. A sealing cover (45) is installed at the bottom of the transfer cylinder (41). The sealing cover (45) is connected to the bottom edge of the transfer cylinder (41) by an electric hinge to control the opening and closing of the bottom of the transfer cylinder (41).

6. The sample collection device for detecting coccidia oocysts according to claim 5, characterized in that: The transfer chamber (105) has a transfer channel (106) extending to the location of the transfer chamber (3) above it. The top of the transfer cylinder (41) is equipped with an end motor (46). The end motor (46) is used to drive the screw inside the transfer cylinder (41) to rotate axially, thereby driving the discharge push block (47) set inside the transfer cylinder (41) to move up and down. The outer peripheral wall of the discharge push block (47) is in close contact with the inner wall of the transfer cylinder (41) so that when the discharge push block (47) moves downward, it can push out all the fecal samples in the transfer cylinder (41) from the opening at the bottom of the transfer cylinder (41).

7. The sample collection device for detecting coccidia oocysts according to claim 5, characterized in that: The rotating ring (31) is detachably fitted with a shielding plate (34) for closing the opening of the placement cavity (302) so that after the collection tube (2) is placed into the placement cavity (302), the user can keep the collection tube (2) in the placement cavity (302) by installing the shielding plate (34).

8. A method for detecting coccidial oocysts, using the sample collection device for detecting coccidial oocysts as described in any one of claims 1-7, characterized in that, Includes the following steps: S1: Insert one end of the sampling rod (1) with the collection groove (101) into a pile of fresh animal feces or the animal rectum to obtain a fecal sample; S2: Take the collection tube (2) containing the fecal sample out of the rotating chamber (3), add saturated urea solution into the collection tube (2) so that the ratio of feces to saturated urea solution in the collection tube (2) is 1:10-1:20, and then stir the feces and saturated urea solution thoroughly to make the feces completely dispersed in the saturated urea solution to form a uniform suspension. S3: Place the collection tube (2) containing the mixed suspension into the centrifuge, set the centrifugation speed of the centrifuge to 1500-2000 rpm, and set the centrifugation time to 5-10 minutes. After centrifugation, the coccidia oocysts will float to the surface of the liquid. S4: Carefully aspirate a layer of liquid from the surface using a pipette or pipette and transfer it to a glass slide. Then, add an appropriate amount of sealing medium to the glass slide and gently cover it with a coverslip to avoid creating air bubbles. S5: Place the prepared slide under a microscope for observation to detect information such as the type and number of coccidia.

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