A specific primer set for differentiating the sex of Eucommia ulmoides and its identification method

By using a specific primer set targeting the Chr10a inversion region of the sex chromosome of Eucommia ulmoides, the problems of low accuracy and poor stability in sex identification of young Eucommia ulmoides were solved, achieving rapid and accurate sex identification and improving breeding efficiency and economic benefits.

CN122081470APending Publication Date: 2026-05-26SHANDONG FOREST & GRASS GERMPLASM RESOURCE CENT (SHANDONG YAOXIANG FOREST FARM) +1
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Patent Information

Application Number
CN202610213233.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-02-13
Publication Date
2026-05-26

AI Technical Summary

Technical Problem

Existing technologies make it difficult to quickly and accurately identify the sex of Eucommia ulmoides in its early stages, resulting in long breeding cycles, low efficiency, and insufficient accuracy and stability of traditional methods.

Method used

A specific primer set was designed to target the Chr10a inversion region of the sex chromosome of Eucommia ulmoides, including primers INV53, INV54, INV55 and INV56. Sexing was achieved by PCR amplification and electrophoresis detection, which is suitable for different genetic backgrounds and cultivation environments.

Benefits of technology

It achieves 100% accuracy in identifying male and female Eucommia ulmoides, is simple and quick, adaptable to different environments, shortens the breeding cycle, and improves resource utilization efficiency and economic benefits.

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Abstract

This invention provides a specific primer set for identifying the sex of Eucommia ulmoides and its identification method, relating to the field of plant molecular identification technology. The specific primer set includes primers INV53, INV54, INV55, and INV56. The upstream primer of primer INV53 is shown in SEQ ID NO.1, and the downstream primer is shown in SEQ ID NO.2; the upstream primer of primer INV54 is shown in SEQ ID NO.3, and the downstream primer is shown in SEQ ID NO.4; the upstream primer of primer INV55 is shown in SEQ ID NO.5, and the downstream primer is shown in SEQ ID NO.6; the upstream primer of primer INV56 is shown in SEQ ID NO.7, and the downstream primer is shown in SEQ ID NO.8. The specific primer set for identifying the sex of Eucommia ulmoides provided by this invention targets the Chr10a inverted region, achieving 100% identification accuracy. It features high specificity and accuracy, is adaptable to samples with different genetic backgrounds and cultivation environments, and solves the problems of low accuracy and poor stability in existing methods.
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