Resonant chip, preparation method and application thereof in preparation of extracellular vesicles

By combining resonant chips with ultrasonic resonance and microfluidic technology, and utilizing micropillar arrays to generate controllable acoustic flow shear force, the low efficiency and safety issues in the preparation of extracellular vesicles in existing technologies have been solved, enabling efficient and safe large-scale preparation and application as a drug carrier.

CN122104422APending Publication Date: 2026-05-29GUANGXI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGXI UNIV
Filing Date
2026-02-10
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Existing technologies are difficult to achieve efficient and safe large-scale preparation of extracellular vesicles. Chemical induction methods have safety concerns, and physical stimulation strategies such as cell membrane compression can cause cell damage. The application of microfluidic technology in upstream induction of cells to efficiently produce extracellular vesicles has not been fully explored.

Method used

By combining resonant chip technology with ultrasonic resonance and microfluidics, a micropillar array chip is fabricated by 3D printing. The micropillar array is driven by a piezoelectric sensor to generate controllable acoustic flow shear force, which stimulates cells non-contactly to increase extracellular vesicle production and avoids cell damage caused by chemical induction and direct mechanical compression.

Benefits of technology

It achieved an approximately 30-fold increase in extracellular vesicle yield, with high-quality vesicles and good cell viability, making it suitable as a drug delivery carrier and possessing the potential for large-scale preparation and industrial production.

✦ Generated by Eureka AI based on patent content.

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Abstract

The application discloses a resonance chip, which comprises a glass substrate, a resonance base, a resonance cover plate and a piezoelectric sensor. The resonance base is arranged on the top surface of the glass substrate, and a microchannel is arranged on the resonance base. One end of the microchannel is provided with a channel inlet, and the other end is provided with a channel outlet. A plurality of micro columns are arranged in the microchannel. The resonance cover plate is arranged on the top surface of the resonance base. The resonance cover plate is provided with a sample inlet corresponding to the channel inlet of the microchannel, and a sample outlet corresponding to the channel outlet of the microchannel. The piezoelectric sensor is arranged on the top surface of the glass substrate. The application further discloses a preparation method of the resonance chip and application thereof. The piezoelectric sensor drives the micro column array to generate controllable acoustic flow shear force to non-contact physically stimulate cells. In the case of maintaining the activity of the cells, the application can realize efficient and safe preparation of extracellular vesicles.
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