An automatic whole blood pretreatment device and method for detecting glycated hemoglobin

Abstract

The application discloses a kind of automatic whole blood pretreatment device and method for detecting glycosylated hemoglobin, including magnet delivery module, sample disc module, magnetic stirring module and reagent delivery module;Wherein the magnet delivery module includes the warehouse for accommodating multiple magnets, the feeder in contact with the magnet outlet of warehouse, the drive of drive feeder, the feeder includes recess, the size of recess is suitable for accommodating one magnet;Drive feeder, so that the feeder drives recess moves between the magnet outlet of warehouse and sample disc module;Sample disc module includes the sample disc for containing sampling tube and the drive for driving sample disc rotation, and sample disc is located below feeder;Magnetic stirring module includes the magnetic stirrer below sample disc;Reagent delivery module includes reagent delivery device above sample disc.The device can automatically perform reagent mixing and other pretreatment, which greatly improves the processing speed.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, and in particular to an automated whole blood pretreatment device and method for detecting glycated hemoglobin. Background Technology

[0002] Existing methods for detecting glycated hemoglobin mainly include high-performance liquid chromatography (HPLC), immunoassay, enzymatic methods, and capillary electrophoresis.

[0003] Currently available fully automated glycated hemoglobin analyzers generally use high-performance liquid chromatography (HPLC). However, the disadvantages of HPLC equipment at present are its high price, limited testing speed (typically 1 minute / test) due to the limitations of chromatographic principles, and detection capacity of no more than 60 samples / hour, resulting in low detection capacity in high-throughput environments.

[0004] Latex agglutination reaction is an immunoassay method. It is simple and rapid, offering fast testing speeds. The standard testing procedure includes sample preparation, reagent and sample mixing, immunoassay and agglutination, absorbance measurement, and result calculation. After completing the sample pretreatment process, a fully automated biochemical analyzer can be used for testing. However, when using a biochemical analyzer to measure glycated hemoglobin, the blood sample needs to be pretreated. This involves manually adding reagents offline before loading the sample, allowing the red blood cells to rupture and release hemoglobin, followed by manual loading for testing. This method cannot achieve fully automated testing, impacting the testing speed and preventing the high-speed, high-throughput advantages of the biochemical analyzer from being fully utilized. Summary of the Invention

[0005] The purpose of this invention is to provide an automated whole blood pretreatment apparatus and method for detecting glycated hemoglobin, so as to at least partially solve the above-mentioned problems of the prior art.

[0006] To achieve the above objectives, one aspect of the present invention provides an automated whole blood pretreatment device for detecting glycated hemoglobin, comprising a magnet dispensing module 1, a sample tray module 2, a magnetic stirring module 3, and a reagent dispensing module 4; wherein The magnet dispensing module 1 includes a housing for accommodating multiple magnets, a feeder that contacts the magnet outlet of the housing, and a driver that drives the feeder. The feeder includes a groove, the size of which is suitable for accommodating one magnet. The driver drives the feeder, causing the feeder to move the groove between the magnet outlet of the housing and the sample tray module 2. The sample tray module 2 includes a sample tray for holding sampling tubes and a driver for driving the sample tray to rotate, and the sample tray is located below the feeder; the magnetic stirring module 3 includes a magnetic stirrer located below the sample tray; the reagent dispensing module 4 includes a reagent dispensing device located above the sample tray.

[0007] Preferably, the hopper includes a track 5 and a packaging box 6. The packaging box 6 is removably installed inside the track 5 via one side of the track 5. The other side of the track 5 includes an opening. The packaging box 6 is used to accommodate a magnet. The size of the opening is suitable for the magnet to pass through. The opening is in contact with the feeder. The feeder includes a single-axis feeding push rod assembly, wherein the single-axis feeding push rod assembly includes a stepper motor 8 and a feeding push rod body 12; the feeding push rod body 12 is in orthogonal contact with the track 5, and the stepper motor 8 drives the feeding push rod body 12 to reciprocate in a direction orthogonal to the track 5; the surface of the feeding push rod body 12 in contact with the track 5 contacts the opening of the track 5 and blocks the opening, and the surface of the feeding push rod body 12 in contact with the track 5 includes a groove.

[0008] Preferably, the feeder further includes an electromagnetic adsorption system 13 disposed on the opposite side of the groove. The electromagnetic adsorption system 13 includes a magnet induction sensor, which is used to determine whether the groove contains the magnet and the polarity of the magnet. The electromagnetic adsorption system 13 generates electromagnetic force to attract the magnet according to the polarity of the magnet when energized, and de-energizes or applies a reverse current to form a repulsive force after the feeding push rod body 12 reaches a preset position above the sample tray.

[0009] Preferably, the feeder further includes a magnetic conveying device fixedly connected to the feeding push rod body 12. The magnetic conveying device includes a compression spring 15 and a magnetic tongue 16. The magnetic tongue 16 protrudes from the surface of the feeding push rod body 12 that contacts the track 5. The end of the magnetic tongue 16 away from the track 5 is connected to the compression spring 15. The magnetic tongue 16 covers the groove when it is not subjected to the elastic force of the compression spring 15.

[0010] Preferably, the feeding push rod body 12 further includes an origin baffle 18, the position of which corresponds to the controller of the stepper motor 8. The origin baffle 18 moves with the feeding push rod body 12 toward the track 5, and when the groove corresponds to the magnet, it triggers the controller of the stepper motor 8 to control the feeding push rod body 12 to stop moving.

[0011] Preferably, it also includes a pressure device that applies a force to the magnet inside the packaging box (6) to bring the magnet closer to the feeding push rod body (12).

[0012] Preferably, the hopper includes at least one column of magnet containers arranged vertically, and a magnet outlet is provided below the at least one column of magnet containers; the feeder includes a distributing turntable 31, the outer surface of the distributing turntable 31 includes strip tracks, one strip track corresponds to one column of magnet containers, each strip track includes at least one groove, and the distributing turntable 31 rotates vertically when driven.

[0013] Preferably, the material distribution turntable 31 includes a material distribution shovel seat 29 below it. The material distribution shovel seat 29 includes shovel teeth and an arc-shaped material channel. The shovel teeth contact the material distribution turntable 31 and shovel the magnet from the groove into the arc-shaped material channel. The lower outlet of the arc-shaped material channel corresponds to the position of the sampling tube.

[0014] Preferably, the material distribution turntable 31 includes multiple elliptical strip tracks.

[0015] Another aspect of the present invention provides an automated whole blood pretreatment method for detecting glycated hemoglobin, applied to the aforementioned automated whole blood pretreatment device for detecting glycated hemoglobin, comprising: Move the feeder so that the groove aligns with the outlet of the chamber, and receive the magnet inside the chamber; move the feeder above the sample tray and throw the magnet into the sampling tube; rotate the sample tray so that the sampling tube is below the reagent dispensing device, and add the glycated hemoglobin detection reagent into the sampling tube through the reagent dispensing device; rotate the sample tray so that the sampling tube is above the magnetic stirrer, and drive the magnet inside the sampling tube to rotate through the magnetic stirrer.

[0016] Compared with the prior art, the present invention has at least the following advantages: This invention provides an automated whole blood pretreatment technology for detecting glycated hemoglobin, which can be applied to methods such as latex agglutination reaction that require reagent mixing to detect glycated hemoglobin. A magnet is automatically delivered into the sampling tube by a feeder, and the detection reagent for glycated hemoglobin is added into the sampling tube by a reagent dispensing device. The sampling tube is automatically stirred by a magnetic stirrer, thereby automatically performing reagent mixing and isochemical pretreatment, which greatly improves the processing speed and helps to give full play to the high-speed and high-throughput advantages of biochemical analyzers. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of an automated whole blood pretreatment device for detecting glycated hemoglobin provided in Embodiment 1 of the present invention.

[0018] Figure 2 This is a schematic diagram of the magnet delivery module provided in Embodiment 1 of the present invention.

[0019] Figure 3 This is a schematic diagram of the magnet transport device provided in Embodiment 1 of the present invention.

[0020] Figure 4 This is a schematic diagram of the magnet delivery module provided in Embodiment 2 of the present invention.

[0021] Figure 5 This is another structural schematic diagram of the magnet delivery module provided in Embodiment 2 of the present invention. Detailed Implementation

[0022] To enable those skilled in the art to better understand the present invention, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of the present invention.

[0023] It should be noted that the terms "first," "second," etc., in the specification, claims, and accompanying drawings of this invention are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be used interchangeably where appropriate to understand the embodiments of the invention described herein. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion; for example, a product or device comprising a series of units is not necessarily limited to those explicitly listed, but may include other units not explicitly listed or inherent to such product or device.

[0024] In this invention, the terms "upper," "lower," "left," "right," "front," "rear," "top," "bottom," "inner," "outer," "middle," "vertical," "horizontal," "lateral," and "longitudinal" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. These terms are primarily for the purpose of better describing the invention and its embodiments, and are not intended to limit the indicated devices, elements, or components to having a specific orientation, or to be constructed and operated in a specific orientation.

[0025] Furthermore, in addition to indicating direction or positional relationship, some of the aforementioned terms may also have other meanings. For example, the term "above" may also be used in certain situations to indicate a dependency or connection. Those skilled in the art can understand the specific meaning of these terms in this invention based on the specific circumstances.

[0026] Furthermore, the terms "installation," "setup," "equipped with," "connection," "linking," and "socketing" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral structure; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium, or an internal connection between two devices, components, or parts. Those skilled in the art can understand the specific meaning of these terms in this invention based on the specific circumstances.

[0027] It should be noted that, unless otherwise specified, the embodiments and features described in the present invention can be combined with each other. The present invention will now be described in detail with reference to the accompanying drawings and embodiments. Example

[0028] This invention provides an automated whole blood pretreatment device for detecting glycated hemoglobin. Figure 1 A schematic diagram of the device is shown. (Reference) Figure 1 As shown, the device includes a magnet dispensing module 1, a sample tray module 2, a magnetic stirring module 3, and a reagent dispensing module 4; wherein... The magnet dispensing module 1 includes a housing for accommodating multiple magnets, a feeder that contacts the magnet outlet of the housing, and a driver that drives the feeder. The feeder includes a groove, the size of which is suitable for accommodating one magnet. The driver drives the feeder, causing the feeder to move the groove between the magnet outlet of the housing and the sample tray module 2. The sample tray module 2 includes a sample tray for holding sampling tubes and a driver for driving the sample tray to rotate, and the sample tray is located below the feeder; The magnetic stirring module 3 includes a magnetic stirrer located below the sample tray; The reagent dispensing module includes a reagent dispensing device located above the sample tray.

[0029] Figure 2 A schematic diagram of the magnet delivery module 1 is shown. (Reference) Figure 2As shown, the hopper and the feeder are arranged horizontally. The hopper includes a track 5 and a packaging box 6. The packaging box 6 is pull-out and installed within the track 5 via one side. The other side of the track 5 includes an opening. The packaging box 6 is used to accommodate a magnet. The size of the opening is suitable for the magnet to pass through. The opening contacts the feeder. The feeder includes a single-axis feeding pusher assembly, which includes a stepper motor 8 and a feeding pusher body 12. The feeding pusher body 12 is in orthogonal contact with the track 5. The stepper motor 8 drives the feeding pusher body 12 to reciprocate in a direction orthogonal to the track 5. The surface of the feeding pusher body 12 that contacts the track 5 contacts and blocks the opening of the track 5. The surface of the feeding pusher body 12 that contacts the track 5 includes a groove.

[0030] In one optional embodiment, the track 5 and the packaging box 6 form a sample feeding track, which is fixedly mounted on the mounting frame 7, and the magnet slides inside the packaging box 6. In another optional embodiment, the device further includes a pressure-applying device that applies a force to the magnet inside the packaging box 6, causing the magnet to move closer to the feeding pusher body 12. This pressure-applying device can apply electromagnetic or mechanical force to the magnet inside the packaging box 6. For example, the side of the feeding pusher body 12 near the track 5 may include an iron block, and the magnetic force between the iron block and the magnet causes the iron block inside the packaging box 6 to move closer to the feeding pusher body 12. Alternatively, a spring, motor, or other device capable of applying a force towards the feeding pusher body 12 to the end of the packaging box 6 away from the feeding pusher body 12 may be provided, pushing the iron block inside the packaging box 6 closer to the feeding pusher body 12.

[0031] refer to Figure 2 As shown, the stepper motor 8 and the feed pusher body 12 can also be mounted on the fixed frame 7. In one example, the feeder includes a stepper motor 8, a gear 9, a rack 10, and a feed pusher body 12. The stepper motor 8 is fixedly mounted on the fixed frame 7. The stepper motor 8 drives the gear 9 to rotate, and the gear 9 drives the rack 10 to move. The feed pusher body 12 can be mounted on the rack 10 and reciprocates with the drive of the stepper motor 8. The feeder may also include a linear guide rail 11 that contacts the feed pusher body 12 and defines the movement of the feed pusher body 12 along the path defined by the linear guide rail 11.

[0032] refer to Figure 2As shown, the feeder also includes an electromagnetic adsorption system 13 disposed on the opposite side of the groove. The electromagnetic adsorption system 13 includes a magnet induction sensor, which is used to determine whether the groove contains the magnet and the polarity of the magnet. The electromagnetic adsorption system 13 generates electromagnetic force to attract the magnet according to the polarity of the magnet when energized. After the feeding pusher body 12 reaches a preset position above the sample tray, the power is cut off or a reverse current is applied to form a repulsive force, causing the magnet to fall into the sampling tube on the sample tray below.

[0033] In an optional embodiment, the feeder further includes a magnetic conveying device fixedly connected to the feed pusher body 12. Figure 3 A schematic diagram of the magnet transport device is shown. (Reference) Figure 3 As shown, the magnet conveying device includes a compression spring 15 and a magnet pressure tongue 16; wherein, the magnet pressure tongue 16 protrudes from the surface of the feeding push rod body 12 that contacts the track 5, and the end of the magnet pressure tongue 16 away from the track 5 is connected to the compression spring 15, and the magnet pressure tongue 16 covers the groove when not subjected to the elastic force of the compression spring 15. In one example, the magnet conveying device also includes a push rod head 14, which is fixedly installed at the end of the feeding push rod body 12, and the end of the compression spring 15 away from the magnet pressure tongue 16 is fixedly installed at the push rod head 14. In one example, the magnet conveying device may further include a pressure tongue track 19, along which the magnet pressure tongue 16 can move.

[0034] refer to Figure 3 As shown, in an optional embodiment, the feeding pusher body 12 further includes a home baffle 18. The position of the home baffle 18 corresponds to the controller 20 of the stepper motor 8. The home baffle 18 moves with the feeding pusher body 12 toward the track 5, and triggers the stepper motor 8 to stop running when the groove corresponds to the magnet, and the magnet enters the groove. (Reference) Figure 3 As shown, the feeding push rod body 12 may also include a traction plate 17. The traction plate 17 can be an iron plate, which is embedded in the feeding push rod body 12. When the feeding push rod body 12 reciprocates, it always corresponds to the exit position of the track 5, attracting the magnet to correspond to the feeding push rod body 12 through the exit of the track 5, and attracting the magnet to enter the groove when the groove corresponds to the magnet.

[0035] In a preferred embodiment, a protective film may be provided on the surface of the magnet to prevent the magnet from contaminating the blood sample collected in the sampling tube.

[0036] In the illustration of this embodiment, the magnet is depicted as a sheet, but it can also be spherical or other shapes suitable for transport and as a magnetic stirring rotor. The chamber track and grooves are configured to accommodate the shape of the magnet. The size of the magnet needs to correspond to the size of the sampling tube. Example

[0037] Embodiment 2 of the present invention provides an automated whole blood pretreatment device for detecting glycated hemoglobin, referring to... Figure 1 As shown, the device includes a magnet dispensing module 1, a sample tray module 2, a magnetic stirring module 3, and a reagent dispensing module 4. The difference from Embodiment 1 is that the structure of the magnet dispensing module 1 is different. The configuration of the other sample tray module 2, magnetic stirring module 3, and reagent dispensing module 4 can refer to Embodiment 1.

[0038] Figure 4 and Figure 5 A schematic diagram of the magnet delivery module 1 in this device is shown. (Reference) Figure 4 and Figure 5 As shown, the basic principle of the magnet dispensing module 1 in this embodiment is a rotating magazine type. The upper magazine includes at least one row of magnet containers arranged vertically, and a magnet outlet is located below the at least one row of magnet containers. The feeder includes a dispensing turntable 31, the outer surface of which includes strip tracks, one strip track corresponding to one row of magnet containers. Each strip track includes at least one groove. The dispensing turntable 31 is driven to rotate vertically. In an optional embodiment, the dispensing turntable 31 includes multiple elliptical strip tracks.

[0039] Each magnet holder accommodates a stack of magnets, and one track corresponds to one stack. The number of tracks and the number of grooves on each track can be flexibly set according to actual needs, so that the position and frequency of magnet placement match the position and rotation speed of the sampling tube. If there is only one stack of magnets, only one track can be set, and one or more grooves can be made on this track; if there are two or more stacks of magnets, a corresponding number of tracks can be set, and one or more grooves can be made on each track. For example, if there are two stacks of magnets, two tracks are set, each track has one groove, and the two grooves are spaced 180 degrees apart; if there are four stacks of magnets, four tracks are set, each track has one groove, and adjacent grooves are spaced 90 degrees apart. Alternatively, multiple grooves can be made on each track, and the angle between adjacent grooves can be evenly distributed according to the total number of grooves on all tracks. For example, if there are two stacks of magnets, two tracks are set, each track has two grooves, and adjacent grooves are spaced 90 degrees apart; if there are four stacks of magnets, four tracks are set, each track has two grooves, and adjacent grooves are spaced 45 degrees apart. In other embodiments, the multiple grooves can also be unevenly distributed, but can be flexibly set according to actual needs.

[0040] In one optional embodiment, a distributing shovel base 29 is included below the distributing turntable 31. The distributing shovel base 29 includes shovel teeth and an arc-shaped material channel. The shovel teeth contact the distributing turntable 31 and shovel the magnet from the groove into the arc-shaped material channel. The lower outlet of the arc-shaped material channel corresponds to the position of the sampling tube.

[0041] In one example, reference Figure 4 As shown, the upper hopper body may include three rows of track bins 21, linear guide rails 22 corresponding to the track bins 21, a hopper pressure block 23 disposed on the side of the hopper body, and side hooks 25 connecting the side of the hopper body and the fixing frame 27. The hopper pressure block 23 can be used to apply pressure to the magnet, causing the magnet to enter the groove. In other optional embodiments, other devices such as springs, motors, and electromagnetic systems can be used to replace the hopper pressure block 23, applying force to the magnet inside the hopper body to lower the magnet and facilitate its entry into the groove.

[0042] The mounting bracket 27 can also be fixedly connected to the dispensing shovel base 29. The track chamber 21 is used to house the magnet assembly 24. The feeder can also include a dispensing plate 26, which is fixedly connected to the dispensing turntable 31 to fix the turntable 31 without hindering its rotation; the dispensing plate 26 can also include fixing devices to fix the chamber above the dispensing turntable 31. The feeder can also include a worm gear reducer motor 32 to provide rotational power to the dispensing turntable 31. In one example, the feeder can also include an encoder disk 28 and an origin sensor 30 to control the start and stop of the worm gear reducer motor 32, causing the dispensing turntable 31 to stop rotating when the groove aligns with the magnet outlet, so as to receive the magnets falling from the magnet delivery module 1, and then allowing the dispensing turntable 31 to continue rotating, delivering the received magnets to the corresponding position and placing them into the sampling tube. Example

[0043] Embodiment 3 of the present invention provides an automated whole blood pretreatment method for detecting glycated hemoglobin, applied to the automated whole blood pretreatment device for detecting glycated hemoglobin provided in Embodiment 1, comprising: Move the feeder so that the groove aligns with the outlet of the bin, and receive the magnet inside the bin; Move the feeder above the sample tray and throw the magnet into the sampling tube; Rotate the sample tray so that the sampling tube is below the reagent dispensing device, and add the glycated hemoglobin detection reagent into the sampling tube through the reagent dispensing device; Rotate the sample tray so that the sampling tube is above the magnetic stirrer, and drive the magnet inside the sampling tube to rotate through the magnetic stirrer.

[0044] refer to Figure 1-3 As shown, the magnet delivery module 1 carries the magnet and moves it above the sample tray module 2. It releases the magnet above the sampling tube as needed, and the magnet falls into the blood collection tube. Reagent is then added via the reagent delivery module 4. The module rotates and moves to directly above the magnetic stirring module 3, where it begins stirring and mixing. There is no direct contact during operation, avoiding contamination, and blood sample pretreatment can be completed in a confined space without manual sample loading / unloading or equipment changes.

[0045] During magnet loading and unloading, the magnet delivery module is the primary operator. In this process, the magnets within the sample feeding track are brought close to the feeding pusher body 12 by the traction plate 17. During sampling, the stepper motor 8 drives the gear 9 to mesh with the rack 10, moving the feeding pusher body 12 towards the track 5 along the linear guide rail 11. The magnet pressure tongue 16, installed inside the pusher head 14, compresses the spring 15 and stops moving after contacting the track 5, exposing the groove on the inner side of the feeding pusher body 12. The feeding pusher body 12 continues to move until the origin stop plate 18 triggers the origin sensor 20, at which point the stepper motor 8 stops, and the magnet enters the groove of the feeding pusher body 12. Simultaneously, the magnet induction sensor of the electromagnetic adsorption system 13 determines whether the magnet has accurately entered the groove and the polarity of the magnet surface. The electromagnetic coil is energized to attract the magnet, preventing it from falling. Subsequently, the stepper motor 8 reverses and drives the feeding push rod body 12 to carry the magnet to the designated blood collection tube position on the sample tray module 2. During the movement, after the magnet moves away a certain distance, the magnet pressure tongue 16 pops out under the action of the compression spring 15, protecting the outside of the groove of the push rod body 12 to prevent the magnet from falling.

[0046] After moving above the designated blood collection tube position, the electromagnetic coil of the electromagnetic adsorption system 13 is de-energized or a reverse current is applied to form a repulsive force, causing the magnet to fall into the blood collection tube and complete the placement. The magnet sensing sensor detects the magnet's state and controls the closed loop. Example

[0047] Embodiment 4 of the present invention provides an automated whole blood pretreatment method for detecting glycated hemoglobin, applied to the automated whole blood pretreatment device for detecting glycated hemoglobin provided in Embodiment 2, comprising: Move the feeder so that the groove aligns with the outlet of the bin, and receive the magnet inside the bin; Move the feeder above the sample tray and throw the magnet into the sampling tube; Rotate the sample tray so that the sampling tube is below the reagent dispensing device, and add the glycated hemoglobin detection reagent into the sampling tube through the reagent dispensing device; Rotate the sample tray so that the sampling tube is above the magnetic stirrer, and drive the magnet inside the sampling tube to rotate through the magnetic stirrer.

[0048] refer to Figure 4 and Figure 5 As shown, after the magnets are filled into the chamber, they are inserted into the distributing plate 26 to complete the preparation. Three elliptical strip tracks are distributed on the distributing turntable 31, each track having a groove for accommodating magnets every 120 degrees. It is easy to understand that, referring to the description in Embodiment 2, the number of tracks and grooves can be flexibly set, as can the angle between the grooves. The worm gear reducer motor 32 drives the distributing turntable 31 to rotate. When a pre-made groove on the turntable comes below the corresponding magnet group 24, the magnet falls into the groove due to its own gravity. After the magnet enters the groove of the distributing turntable 31, the turntable continues to rotate. When it reaches the distributing shovel seat 29, the magnet separates from the material tray after passing through the shovel teeth and falls from the bottom into the test tube through the arc-shaped material channel of the distributing plate 26. The entire separation structure is symmetrical, allowing the distributing turntable 31 to dispense magnets in both left and right directions via the distributing shovel seat 29 when the turntable rotates in different directions.

[0049] The automated whole blood pretreatment device for detecting glycated hemoglobin provided in this invention can be applied to methods requiring reagent mixing for glycated hemoglobin detection, such as latex agglutination reaction. Installed on, for example, a fully automated biochemical analyzer, the device automatically delivers a magnet into the sampling tube via a feeder, adds glycated hemoglobin detection reagents into the sampling tube via a reagent dispensing device, and automatically stirs the sampling tube using a magnetic stirrer. This automatically performs reagent mixing and isochemical pretreatment, greatly improving the processing speed and allowing the high-speed, high-throughput advantages of the biochemical analyzer to be fully utilized. Example

[0050] Embodiment 5 of the present invention provides an automated whole blood pretreatment device for detecting glycated hemoglobin. The difference from Embodiment 1 is that the structure of the magnet dispensing module 1 is different. The settings of the other sample tray module 2, magnetic stirring module 3 and reagent dispensing module 4 can refer to Embodiment 1.

[0051] The magnet delivery module 1 in this embodiment 5 may include the bin body in embodiment 2. The magnet falls downward under the action of gravity and enters the groove of the feeder through the bin body outlet. The difference between the feeder in this embodiment and the feeder in embodiment 1 is that the groove of the feeder in this embodiment is located on the upper surface of the feeding push rod body 12, and the groove of the feeding push rod body 12 can rotate in the vertical direction. When the groove of the feeding push rod body 12 carries the magnet to the preset position, the groove is rotated to make the magnet fall into the sampling tube.

[0052] Other features and detailed descriptions of the magnet delivery module 1 in this embodiment can be found in the relevant descriptions in Embodiments 1 and 2. For example, this embodiment may also include an electromagnetic adsorption system 13 disposed on the opposite side of the groove. When the groove is rotated to align with the sampling tube, power is cut off or a reverse current is applied to generate a repulsive force, causing the magnet to fall into the sampling tube. For any parts that do not contradict the scheme of this embodiment, please refer to Embodiments 1 and 2, which will not be repeated here.

[0053] The automated whole blood pretreatment device for detecting glycated hemoglobin provided in this invention can be applied to methods requiring reagent mixing for glycated hemoglobin detection, such as latex agglutination reaction. Installed on, for example, a fully automated biochemical analyzer, the device automatically delivers a magnet into the sampling tube via a feeder, adds glycated hemoglobin detection reagents into the sampling tube via a reagent dispensing device, and automatically stirs the sampling tube using a magnetic stirrer. This automatically performs reagent mixing and isochemical pretreatment, greatly improving the processing speed and allowing the high-speed, high-throughput advantages of the biochemical analyzer to be fully utilized.

[0054] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them. Those skilled in the art should understand that modifications can be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An automated whole blood pretreatment device for detecting glycated hemoglobin, characterized in that, It includes a magnet dispensing module (1), a sample tray module (2), a magnetic stirring module (3), and a reagent dispensing module (4); among which The magnet dispensing module (1) includes a bin for accommodating multiple magnets, a feeder that contacts the magnet outlet of the bin, and a driver for driving the feeder. The feeder includes a groove, the size of which is suitable for accommodating one magnet. The driver drives the feeder so that the feeder moves the groove between the magnet outlet of the bin and the sample tray module (2). The sample tray module (2) includes a sample tray for holding sampling tubes and a driver for driving the sample tray to rotate, the sample tray being located below the feeder; The magnetic stirring module (3) includes a magnetic stirrer located below the sample tray; the reagent dispensing module (4) includes a reagent dispensing device located above the sample tray.

2. The automated whole blood pretreatment device for detecting glycated hemoglobin according to claim 1, characterized in that, The hopper includes a track (5) and a packaging box (6). The packaging box (6) is removably installed in the track (5) through one side of the track (5). The other side of the track (5) includes an opening. The packaging box (6) is used to accommodate a magnet. The size of the opening is suitable for the magnet to pass through. The opening is in contact with the feeder. The feeder includes a single-axis feeding push rod assembly, wherein the single-axis feeding push rod assembly includes a stepper motor (8) and a feeding push rod body (12); the feeding push rod body (12) is in orthogonal contact with the track (5), and the stepper motor (8) drives the feeding push rod body (12) to reciprocate in a direction orthogonal to the track (5); the surface of the feeding push rod body (12) in contact with the track (5) contacts the opening of the track (5) and blocks the opening, and the surface of the feeding push rod body (12) in contact with the track (5) includes a groove.

3. The automated whole blood pretreatment device for detecting glycated hemoglobin according to claim 2, characterized in that, The feeder also includes an electromagnetic adsorption system (13) disposed on the opposite side of the groove. The electromagnetic adsorption system (13) includes a magnet sensor, which is used to determine whether the groove contains the magnet and the polarity of the magnet. The electromagnetic adsorption system (13) generates electromagnetic force to attract the magnet by energizing it according to the polarity of the magnet. After the feed pusher body (12) reaches a preset position above the sample tray, the power is cut off or a reverse current is applied to form a repulsive force.

4. The automated whole blood pretreatment device for detecting glycated hemoglobin according to claim 2, characterized in that, The feeder also includes a magnetic conveying device fixedly connected to the feed push rod body (12), the magnetic conveying device including a compression spring (15) and a magnetic pressure tongue (16); wherein, The magnetic tongue (16) protrudes from the surface of the feeding push rod body (12) that contacts the track (5). The end of the magnetic tongue (16) away from the track (5) is connected to the compression spring (15). The magnetic tongue (16) covers the groove when it is not subjected to the elastic force of the compression spring (15).

5. The automated whole blood pretreatment apparatus for detecting glycated hemoglobin according to any one of claims 2-4, characterized in that, The feeding push rod body (12) also includes an origin baffle (18). The position of the origin baffle (18) corresponds to the controller of the stepper motor (8). The origin baffle (18) moves with the feeding push rod body (12) toward the track (5) and triggers the controller of the stepper motor (8) to control the feeding push rod body (12) to stop moving when the groove corresponds to the magnet.

6. The automated whole blood pretreatment apparatus for detecting glycated hemoglobin according to any one of claims 2-4, characterized in that, It also includes a pressure device that applies a force to the magnet inside the packaging box (6) to bring the magnet closer to the feeding push rod body (12).

7. The automated whole blood pretreatment device for detecting glycated hemoglobin according to claim 1, characterized in that, The container includes at least one column of magnet containers arranged vertically, and a magnet outlet is located below the at least one column of magnet containers; The feeder includes a distributing turntable (31), the outer surface of which includes strip tracks, one strip track corresponding to one column of magnet holders, each strip track including at least one groove, and the distributing turntable (31) is driven to rotate in the vertical direction.

8. The automated whole blood pretreatment device for detecting glycated hemoglobin according to claim 7, characterized in that, Below the material distribution turntable (31) is a material distribution shovel seat (29), which includes shovel teeth and an arc-shaped material channel. The shovel teeth contact the material distribution turntable (31) and shovel the magnet from the groove into the arc-shaped material channel. The lower outlet of the arc-shaped material channel corresponds to the position of the sampling tube.

9. The automated whole blood pretreatment device for detecting glycated hemoglobin according to claim 8, characterized in that, The material distribution turntable (31) includes multiple elliptical strip tracks.

10. An automated whole blood pretreatment method for detecting glycated hemoglobin, applied to the apparatus according to any one of claims 1-9, characterized in that, include: Move the feeder so that the groove aligns with the outlet of the bin, and receive the magnet inside the bin; Move the feeder above the sample tray and throw the magnet into the sampling tube; Rotate the sample tray so that the sampling tube is below the reagent dispensing device, and add the glycated hemoglobin detection reagent into the sampling tube through the reagent dispensing device; Rotate the sample tray so that the sampling tube is above the magnetic stirrer, and drive the magnet inside the sampling tube to rotate through the magnetic stirrer.

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