Device and method for measuring the degree of saccharification

Abstract

The present invention relates to a saccharification degree measuring device and a measuring method. According to the particle measuring device of the present invention, wherein, including: a power supply unit for applying a voltage; a chip formed with a plurality of electrodes arranged in order on a micro flow path through which a fluid passes; a circuit unit printed with at least a part of a bridge circuit forming a predetermined electrical state with the plurality of electrodes formed on the chip; and a measuring unit, the power supply unit applies a voltage to the circuit unit, the measuring unit is used to measure the change of the output signal of the bridge circuit caused by the particles in the fluid passing through the micro flow path. According to the present invention, the change of the electrical signal can be used to accurately measure the micro particles in the solution, and the size and time of the change of the electrical signal can be used to accurately measure the properties of the micro particles.

Description

[0001] This application is a divisional application of Chinese Patent Application No. 202280092076.4 (PCT / KR2022 / 019905), filed on December 8, 2022, entitled "Method for measuring particles in solution and apparatus for performing the method". Technical Field

[0002] This invention relates to a device and method for measuring the degree of saccharification. Background Technology

[0003] Various particle counting techniques are being developed to analyze the purity or cellular units of solutions containing fine particles.

[0004] For example, light scattering methods calculate the number and size of particles by correlating the amount of light detected after it is directly transmitted into a sample and reflected by the surface of particles in the sample with the size or refractive index of the measured particles. However, when the particle surface has an irregular shape, the degree of light reflection may vary depending on the surface, which can lead to errors.

[0005] On the other hand, the method of using resistance involves passing a conductive sample through the gap between electrodes and using the electrical pulses generated at this time to count the particles.

[0006] Typically, impedance-based flow cytometry uses the principle of resistance to measure particle size and distribution. When particles dispersed in an electrolyte solution pass through a small orifice in a microflow path, the resistance between two electrodes flowing with a constant current increases, and the resulting potential difference is measured to convert it into particle size and distribution.

[0007] However, this type of flow cytometry analysis has the disadvantage of requiring operators trained in a well-controlled microenvironment and being difficult to use outside of a laboratory setting due to the complexity of the equipment.

[0008] Therefore, there is a need to design simpler structures for analysis or diagnostics for portable purposes. Summary of the Invention

[0009] The problem to be solved

[0010] The purpose of this invention is to provide a simpler method for measuring particles in a solution.

[0011] More specifically, the object of the present invention is to provide a method for measuring particles in a solution by constructing a chip with micro-flow paths and a bridge circuit, and processing the output signal individually.

[0012] More specifically, the object of the present invention is to provide a method for counting the passage of fine particles using an electrical signal sensed by a Wheatstone bridge.

[0013] More specifically, the object of the present invention is to provide a method for measuring the characteristics of particles using the magnitude and time of changes in the electrical state of input and output signals.

[0014] Methods for solving problems

[0015] To solve the above-mentioned technical problems, a particle measuring device according to the present invention includes: a power supply unit for applying a voltage; a chip having a plurality of electrodes arranged sequentially in a micro-flow path through which a fluid passes; a circuit unit having at least a portion of a bridge circuit printed thereon, which forms a predetermined electrical state with the plurality of electrodes formed on the chip; and a measuring unit, wherein the power supply unit applies a voltage to the circuit unit, and the measuring unit is used to measure the change in the output signal of the bridge circuit caused by particles in the fluid passing through the micro-flow path.

[0016] The measuring unit detects the phase transition of the electrical signal when the particle is located between the arranged electrodes, and counts the particles in the fluid by the number of phase transitions.

[0017] Preferably, the measuring unit calculates the size of the particles in the fluid based on the amplitude of the phase change electrical signal.

[0018] Preferably, it further includes a signal conversion unit that converts the electrical signal applied from the power supply unit into an AC signal, amplifies the converted AC signal, and adjusts it to a predetermined offset. The measurement unit uses the output signal measured on the bridge circuit after the electrical signal with the adjusted offset is applied to measure the change in the electrical state.

[0019] Preferably, the measuring unit uses the original electrical signal after adjustment and offset to multiply the output signal for amplification, removes specific frequency signals from the amplified output signal and converts it to DC, and detects the electrical phase value signal generated by the change in the electrical state.

[0020] Preferably, the frequency to be removed is set to a frequency that is at least similar to the frequency of the original electrical signal.

[0021] Preferably, the measuring unit uses the generation period of the DC-DC output signal to calculate the time it takes for the particle to pass through the flow path.

[0022] To solve the aforementioned technical problem, the particle measurement method according to the present invention includes the steps of: applying a voltage to a bridge circuit in which a plurality of electrodes arranged sequentially in a micro-flow path through which a fluid passes to form a predetermined electrical state; and measuring the change in the output signal of the bridge circuit caused by particles in the fluid passing through the micro-flow path.

[0023] Preferably, the measurement step involves measuring the phase transition of the electrical signal when the particle is located between the arranged electrodes, and counting the number of phase transitions of the particles in the fluid.

[0024] Preferably, the measurement step uses the magnitude of the phase change electrical signal to calculate the size of particles in the fluid.

[0025] Preferably, the step of applying voltage includes: converting an electrical signal into an AC signal and then amplifying the converted AC signal; adjusting the amplified electrical signal to a predetermined offset; and applying the offset electrical signal to the bridge circuit.

[0026] Preferably, the measurement step includes: calculating the difference between the output signals of the bridge circuit and amplifying the difference; multiplying the amplified signals of the difference using the original electrical signal with the adjusted offset; and removing specific frequency signals from the multiplied and amplified signals and converting them to DC.

[0027] Invention Effects

[0028] According to the present invention, fine particles in a solution can be accurately measured by utilizing changes in electrical signals.

[0029] Furthermore, the magnitude and timing of changes in electrical signals can be used to more accurately measure the properties of fine particles.

[0030] Furthermore, this invention, through its simple circuit structure, can effectively measure particle characteristics even at relatively low voltages.

[0031] Furthermore, this invention quantifies the biological characteristics of particles measured using personal reference values, which can then be directly applied to clinical diagnosis.

[0032] Furthermore, the present invention, through the miniaturization of the measuring device, is easily popularized as a portable measuring device. Attached Figure Description

[0033] Figure 1 This is a diagram illustrating the structure of a device for measuring particle values ​​according to the present invention.

[0034] Figure 2 This is a diagram showing the structure of the power supply section of the apparatus for measuring particle values ​​according to the present invention.

[0035] Figures 3a to 3c This is a diagram illustrating the processing procedure of the power supply section for the input electrical signal according to the present invention.

[0036] Figure 4 This is a diagram showing the structure of the measuring section of the apparatus for measuring particle values ​​according to the present invention.

[0037] Figures 5a to 5d This is a diagram illustrating the processing procedure of the measurement unit for the output electrical signal according to the present invention.

[0038] Figures 6a to 6d This is a diagram illustrating the detection process of the electrical signal generated by the particles according to the present invention.

[0039] Figure 7 This is a graph illustrating the characteristics of the measured signal used for measuring particle values ​​according to the present invention.

[0040] Figure 8 This is a diagram illustrating the characteristics of an electrode structure for measuring particle values ​​according to the present invention.

[0041] Figure 9 This is a flowchart illustrating a method for measuring particle values ​​according to the present invention. Detailed Implementation

[0042] The following description is merely illustrative of the principles of the invention. Therefore, those skilled in the art to which this invention pertains can invent various devices that, while not explicitly described or illustrated in the specification, embody the principles of the invention and are included within its concept and scope. Furthermore, all terms, conditions, and embodiments listed in this specification are for the purpose of clearly understanding the concept of the invention and are not intended to limit the specifically listed embodiments and states.

[0043] The objectives, features, and advantages described herein will become more apparent from the following detailed description in conjunction with the accompanying drawings, thereby enabling those skilled in the art to readily implement the technical concept of the invention.

[0044] Furthermore, in describing this invention, if it is determined that a detailed description of the known technologies related to this invention would obscure the main points of the invention, such detailed description will be omitted. Hereinafter, preferred embodiments of the invention will be described in detail with reference to the accompanying drawings.

[0045] Figure 1 This is a diagram showing the structure of a device 100 for measuring particle values ​​according to the present invention.

[0046] Reference Figure 1The particle measuring device 100 according to the present invention includes: a power supply unit 110 for power supply, a circuit unit 120 with printed circuits, and a measuring unit 130 for detecting and measuring electrical signals output from the circuit unit 120.

[0047] In addition, the particle measuring device 100 may be configured to include a micro-flow path formed on a substrate between an externally open inlet and an outlet, and a measuring chip 140 in contact with a plurality of electrodes arranged in the flow path.

[0048] The chip 140 can be configured to be detachable from the particle measuring device 100, thereby allowing the user to measure particles in the solution to be measured while replacing the chip each time a measurement is performed.

[0049] Furthermore, the electrodes on the inserted chip 140 together with the electrodes of the circuit section form a bridge circuit, thereby allowing the measurement section 130 to detect more subtle changes in electrical characteristics.

[0050] In this embodiment, the measuring unit 130 measures the electrical signal output from the bridge circuit in a basic state where the electrolyte solution flows through a micro-flow path but the particles in the solution do not pass between the electrodes. The measuring unit 130 detects particles by measuring the change in the electrical signal relative to the measured signal caused by changes in electrical characteristics such as impedance when particles in the solution pass between the electrodes in the flow path.

[0051] Specifically, when particles in the solution are located between the arranged electrodes, a phase transition of the electrical signal can be detected, and the number of particles in the fluid can be counted by the number of times the phase transition signal occurs.

[0052] At this time, the particle measuring device 100 according to this embodiment can also operate with a DC voltage power supply from a portable power supply device such as a battery, and a preprocessing process for converting the power applied from the power supply unit to AC can be performed before application.

[0053] refer to Figure 2 The preprocessing of the input signal will be explained in more detail.

[0054] Figure 2 This is a block diagram showing the detailed structure of the power supply section according to this embodiment.

[0055] Reference Figure 2 In addition to the power supply 112, the power supply unit also includes a function generator 114 and a signal conversion unit 116.

[0056] Typically, in the case of power supplies for portable devices such as batteries, electrical signals are output in the form of direct current because the terminals maintain a predetermined polarity. Therefore, in this embodiment, the function generator 114 converts the direct current signal into an alternating current signal so that changes in composition such as the phase of the particles can be clearly detected from the potential difference provided by the power supply 112.

[0057] Specifically, the function generator 114 converts the applied voltage into a periodic signal with a continuous waveform having the period of a determined function. The function generator 114 can be implemented in various forms, such as a microcontroller unit, a function generating device, etc., as needed.

[0058] Next, the signal conversion unit 116 amplifies the AC signal with a continuous waveform and adjusts it to a predetermined offset. By adjusting the offset, the amplified AC signal is ultimately converted into a form with repeating positive and negative voltage values, thus having a sine wave form. The periodic repetition of the positive and negative voltage values ​​in the AC signal prevents the electrolysis of the analytical solution by electricity through the electrodes.

[0059] The signal converted during the above signal processing is described in more detail with reference to Figure 3.

[0060] Reference Figure 3a As mentioned above, the voltage output from a battery, primarily used in portable devices, is a constant signal in DC form. Without a periodic DC signal, it is difficult to detect electrical signals with subtle phase differences due to the properties of the conductive material.

[0061] Therefore, the function generator 114 can convert the DC signal from the power supply 112 into a continuous waveform and convert it into a waveform such as... Figure 3b A periodic signal in the form of a signal.

[0062] Next, the signal conversion unit 116 amplifies the input signal to increase the amount of change in the electrical signal output from the function generator 114, while adjusting the offset to have a sine wave form, thereby generating a signal such as... Figure 3c The final input signal is shown.

[0063] That is, the power supply unit 110 according to the present invention solves the problems of extreme concentration of ions in the electrolyte, electrolysis of the solution, and increased resistance in the unidirectional electrical signal of batteries commonly used in portable devices by converting AC signals. Moreover, the flow of particles in the electrolyte solution can be detected by using the periodic component of the periodic signal of the sine wave.

[0064] After the periodic signal generated by the above process is applied to the circuit section 120, the measurement section 130, as an electrical signal output from the bridge circuit, preferably detects the change in voltage amplitude over time.

[0065] In this embodiment, the bridge circuit is a Wheatstone bridge, which can be designed with multiple resistors and electrodes whose impedances are balanced. Therefore, compared with detecting absolute signal values, detection can be performed more sensitively by using the electrical signals generated by state changes within the bridge circuit.

[0066] However, the electrical signal detected in the bridge circuit is also sensitive to noise. Therefore, the measurement unit 130 according to this embodiment needs to accurately distinguish the compositional changes of the specific particles to be detected from the changes in the signal.

[0067] Therefore, the measuring unit 130 according to the present invention includes components for accurate detection.

[0068] Reference Figure 4 The measurement unit 130 includes a buffer 132, an amplifier 134, a multiplier 136, and a filter 138.

[0069] The buffer 132 utilizes the characteristic of near-infinite input impedance to input the output signal from the bridge circuit to the amplifier 134 almost exactly as it is, without being affected by the components of other circuits in the measurement section 130.

[0070] The function of amplifier 134 is to calculate the difference between the input signals and amplify that difference. The input signals may contain various noises from the solution; therefore, noise is removed first by difference amplification, and only the asymmetric signal value generated from the particles is extracted. The extracted signal is then input to multiplier 136.

[0071] Next, multiplier 136 multiplies and amplifies the output signal of amplifier 134. Specifically, amplification can be achieved by multiplying the original electrical signal in the form of a sine wave with adjusted offset by the output signal. The original signal is used because it has the same frequency as the output signal, and other signals with the same frequency besides the original signal can also be used as needed.

[0072] When particles in the solution pass through the flow path and are positioned between electrodes, the impedance between the electrodes may increase instantaneously due to the particles. Furthermore, because the particles act as capacitors, a potential difference may occur. Therefore, the amplitude and phase of the voltage signal may change.

[0073] Therefore, multiplier 136 amplifies the waveform signal by multiplying the original electrical signal with the output signal to extract the signal with a phase difference, while simultaneously constanting the phase value. The output of multiplier 136 is calculated as the sum of an AC wave with frequency as the variable and a DC wave affected by the phase difference. Therefore, in order to ultimately extract only the phase difference generated by the particles, filter 138 filters the AC wave in the output signal of multiplier 136.

[0074] That is, the processing procedure for extracting the pure target signal generated by the particles from the output signal detected by the above-mentioned measuring unit 130 will be described in more detail with reference to FIG5.

[0075] Reference Figure 5a The electrical signal output from the initial Wheatstone bridge may have complex waveforms that cannot be explained by the differences in electrical properties between the solution and the particles.

[0076] Therefore, buffer 132 converts the output signal into an interpretable waveform by compensating for signal bounce in the output signal. The converted signal has Figure 5b As shown, the signal can have a form close to a sine wave.

[0077] At this time, buffer 132 utilizes its near-infinite input impedance to connect dissimilar circuits while ensuring that the measured signal value is not lost. The signal value after buffering is amplified by amplifier 134 to remove noise for the first time. Changes in electrical component caused by particles contained in the signal are amplified and extracted using multiplier 136. In this embodiment, for signal amplification and extraction, the input signal in the form of a sine wave after adjustment and offset of power supply unit 110 can be multiplied by the output signal to obtain the input signal. Figure 5c The type of amplified signal shown can be output from multiplier 136.

[0078] When the amplified signal, after being compensated by a buffer, passes through the multiplication circuit, the frequency components and the DC waveform in constant form, which corresponds to the phase difference, mix. Filter 138 detects the minute phase difference signal caused by the influence of particles by removing the frequency components from the signal.

[0079] That is, after removing the frequency components from the signal, the remaining signal contains residual elements such as... Figure 5d The signal shown has a phase difference.

[0080] The output signal, after removing the component corresponding to the frequency of the input signal, is converted into DC in the same form as the signal originally applied from the power source. Therefore, the measuring unit 130 can extract the component based on the changes in particles within the signal more clearly.

[0081] When a particle momentarily sits on the electrode and then disappears, the signal output from filter 138 has a specific waveform, which is used to determine whether the particle has passed through.

[0082] The generation process of the specific phase transition waveform signal detected as particles pass through in this embodiment will be described in more detail below with reference to FIG6.

[0083] First, refer to Figure 6aBefore the particles pass through the electrodes, the voltage signals in the bridge circuit are in equilibrium and are in phase with the input electrical signal. Therefore, the signal output from filter 138 has a DC waveform as is.

[0084] Then, as the particle advances further and is positioned between the first and second electrodes, a voltage drop occurs because the particle acts as a resistor or capacitor between the respective electrodes. Consequently, the phase (θ1) of the voltage (V1) decreases compared to the phase (θ2) of the voltage (V2). Therefore, the phase-change electrical signal can be detected by filtering 138 to remove a portion of the waveform of the signal with the same frequency as the input signal (such as...). Figure 6b (As shown). Alternatively, the waveform can be detected by removing signals of similar frequencies within a predetermined range from the input signal.

[0085] Next, when the particle passes through the first electrode again and is positioned between the second and third electrodes, a voltage drop occurs between them, and a phase difference appears between the phase (θ2) of voltage (V2) and the phase (θ1) of voltage (V1). Therefore, a phase difference reappears, and the phase of V1 becomes greater than the phase of V2, thus detecting the particle. Figure 6c Signals in the form of...

[0086] When all the last particles have passed through the electrode, the system returns to its basic state and is detected. Figure 6d The signal is in the form shown.

[0087] Furthermore, when the particles are cells and located between electrodes due to the nature of the cells, they can act as capacitors. Therefore, the size or characteristics of the particles will affect the capacitor, and this characteristic will be reflected in the signal.

[0088] Therefore, in this embodiment, the number of particles can be counted by detecting the waveform of continuous particles, and the size of the particles can be calculated using the size of the waveform.

[0089] Reference Figure 7 The wavelength of the signal can be calculated based on the passage time of the particles. Furthermore, as mentioned above, since the particles act as capacitors, the signal magnitude is proportional to the voltage change; therefore, the signal magnitude can also be calculated based on the size of the particles.

[0090] Furthermore, since the aforementioned signal is generated from the moment the particle begins to pass through the electrode until it completes its passage, additional particle characteristics can be calculated by converting the time characteristics of the signal or the passing speed of the particle.

[0091] For example, when particles in a solution are elastic like cells, they can change shape and size according to their original stiffness, and can pass through channels with a width smaller than their original size by reducing the diameter of the particle itself. However, when cells in the human body bind with various substances or become physically rigid due to aging, their elasticity decreases and they become harder.

[0092] Cells with increased rigidity take longer to pass through channels of the same width. According to the particle measurement device of this embodiment, the characteristics of cells can be determined by the proportional relationship between the passage time (wavelength) and factors affecting rigidity, and biological judgments such as diagnosis can be performed.

[0093] For example, red blood cells in the blood become more rigid due to the influence of glycated hemoglobin, so they require more time to pass through a channel of the same width. According to the particle measuring device of this embodiment, the glycation level of a single red blood cell can be determined by the ratio of passage time to glycated hemoglobin level.

[0094] Additionally, refer to Figure 7 and Figure 8 The fluid forms a pattern of fine electrodes, and the gap d between the electrodes can be calculated in advance. Therefore, the passing speed of the electrodes can also be calculated from the wavelength b of the waveform.

[0095] According to the present embodiment, the measurement unit 130 can use the characteristics of the DC signal output from the filter 138 to count the number of particles, and can also use the magnitude or length of the signal to distinguish particles by the unique characteristics of the particles.

[0096] Below, refer to Figure 9 This describes the particle measurement method according to this embodiment.

[0097] Reference Figure 9 First, in order to apply voltage to a bridge circuit that forms a predetermined electrical state by multiple electrodes arranged sequentially on a micro-flow path through which the fluid flows, the electrical signal is first converted into an AC signal and then amplified.

[0098] Next, the amplified electrical signal is adjusted to a predetermined offset to transform it into a sine wave (S200).

[0099] The preprocessed input signal is applied to the bridge circuit (S300).

[0100] Next, the change in the electrical state of the bridge circuit due to the passage of particles in the fluid through the micro-flow path is measured based on the output electrical signal (S400). Specifically, for measurement purposes, only the bouncing signal caused by the difference in characteristics between the particles and the solution can be extracted and amplified from some of the output signals from the bridge circuit.

[0101] By removing specific frequency signals from the amplified output signal and ultimately converting it to DC, the fine signals generated when particles are located between electrodes arranged in the flow path can be detected, and the number of particles in the fluid can be counted based on the number of times the detected signals occur.

[0102] Furthermore, the size of particles in the fluid can be calculated using the magnitude of the phase-change electrical signal, while the transit time can be calculated using the amplitude of the electrical signal.

[0103] According to the present invention, changes in electrical signals can be used to accurately measure fine particles in a solution.

[0104] Furthermore, by utilizing the magnitude and timing of changes in electrical signals, the properties of fine particles can be measured more accurately.

[0105] Furthermore, this invention, through its simple circuit structure, enables efficient measurement of particle properties even at relatively low voltages.

[0106] The degree of saccharification can be easily measured by utilizing the changes in the physical properties of the particles caused by saccharification.

[0107] Furthermore, by calculating hardness through the time it takes for each particle to pass through the microchannel, the degree of saccharification can be measured more consistently against external and human factors compared to measuring devices using biochemical techniques.

[0108] In addition, the present invention can identify the minute electrical changes generated when particles pass through through a simple circuit structure, thereby measuring the degree of saccharification of the particles.

[0109] Furthermore, this invention corrects the measured particle values ​​using personal reference values, thus enabling direct application to clinical diagnosis.

[0110] Furthermore, the various embodiments described herein can be implemented, for example, using software, hardware, or a combination thereof, in a recording medium readable by a computer or similar device.

[0111] Depending on the hardware implementation, the embodiments described herein can be implemented using at least one of the following: application-specific integrated circuits (ASICs), digital signal processors (DSPs), digital signal processing devices (DSPDs), programmable logic devices (PLDs), field-programmable gate arrays (FPGAs), processors, controllers, microcontrollers, microprocessors, and electronic units for performing other functions. In some cases, the embodiments described herein can be implemented by the control module itself.

[0112] According to the software implementation, the embodiments of the processes and functions described in this specification can be implemented by other software modules. Each software module can perform more than one function and operation described in this specification. The software code can be implemented using a software application written in a suitable programming language. The software code can be stored in a storage module and executed by a control module.

[0113] The above is only used to illustrate the technical spirit of the present invention, and those skilled in the art can make various modifications, changes and substitutions to it without departing from the essential characteristics of the present invention.

[0114] Therefore, the embodiments and drawings disclosed in this invention are not intended to limit but rather to explain the technical spirit of the invention, and the scope of the technical spirit of the invention should not be limited by the above embodiments and drawings. Furthermore, it should be understood that the scope of protection of this invention should be interpreted according to the scope of the appended claims, and all technical spirit within the same scope are included within the scope of the claims of this invention.

Claims

1. A device for measuring the degree of saccharification, characterized in that, include: The power supply section is used to apply voltage; The chip has a first electrode, a second electrode, and a third electrode arranged sequentially in a micro-flow path through which fluid passes; The circuit section includes circuit elements connected to the first electrode, the second electrode, and the third electrode to form a bridge circuit with the first electrode, the second electrode, and the third electrode, and the circuit section is supplied with voltage from the power supply section; as well as The measuring unit is used to measure the change in the output signal of the bridge circuit caused by particles in the fluid passing through the micro-flow path. The microchannel has a width smaller than the original size of the cell when the particles are cells. The measuring unit calculates the transit time of the particle in the micro-flow path based on the change in the output signal. The measuring unit uses the passage time to calculate the stiffness of the particle. The measuring unit determines the degree of saccharification of the particles based on the stiffness.

2. The apparatus for measuring the degree of saccharification according to claim 1, characterized in that, The particles are red blood cells from the blood. The measuring unit uses the ratio of transit time to glycated hemoglobin levels to determine the degree of glycation of a single red blood cell.

3. The apparatus for measuring the degree of saccharification according to claim 1 or 2, characterized in that, The measuring unit calculates the passage time based on the fluid passage sequence, according to the first change in the output signal when the particle in the fluid is located between the first electrode and the second electrode, and the second change in the output signal when the particle is located between the second electrode and the third electrode.

4. The apparatus for measuring the degree of saccharification according to any one of claims 1 to 3, characterized in that, The measuring unit detects the phase change of the electrical signal caused by the particle being located between the electrodes, and calculates the passage time using the generation period of the phase change.

5. The apparatus for measuring the degree of saccharification according to any one of claims 1 to 4, characterized in that, The saccharification degree measuring device further includes a signal conversion unit, which converts the electrical signal applied from the power supply unit into an AC signal, amplifies the converted AC signal, and adjusts it to a predetermined offset. The measuring unit measures the change in electrical state in the output signal measured on the bridge circuit after applying an electrical signal whose offset is adjusted by the signal conversion unit.

6. The apparatus for measuring the degree of saccharification according to claim 5, characterized in that, The measuring unit amplifies the output signal using the original electrical signal after the offset is adjusted by the signal conversion unit, removes specific frequency signals from the amplified output signal and DC-converts it, and calculates the passage time using the generation period of the DC-converted output signal.

7. The apparatus for measuring the degree of saccharification according to any one of claims 1 to 6, characterized in that, The measuring unit also calculates the size of the particle based on the amplitude of the change in the output signal, and calculates the stiffness based on the size and the passage time.

8. A method for measuring the degree of saccharification, characterized in that, Includes the following steps: A voltage is applied to the circuit section, wherein the circuit section is connected in such a way that it forms a bridge circuit with a first electrode, a second electrode, and a third electrode arranged sequentially on a micro-flow path on the chip; and The change in the output signal of the bridge circuit is measured due to particles in the fluid passing through the microflow path; The transit time of the particles in the microflow path is calculated based on the changes in the output signal. The stiffness of the particle is calculated using the passage time; and The degree of saccharification of the particles is determined based on the stiffness. The microflow path has a width smaller than the original size of the cell when the particle is a cell.

9. The method for measuring the degree of saccharification according to claim 8, characterized in that, The particles are red blood cells from the blood. In the determination step, the degree of glycation of a single red blood cell is determined by using the ratio of the time to the amount of glycated hemoglobin.

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