A biochemical testing device

Abstract

The application discloses a biochemical detection device and belongs to the technical field of rapid diagnosis. The biochemical detection device comprises a substrate and is provided with a liquid channel, a calibration channel and a gas channel on the substrate. The liquid channel comprises a liquid inlet channel and a reaction channel which are communicated with each other. The calibration channel is communicated with the reaction channel, and calibration reagent can flow into the reaction channel through the calibration channel. The gas channel is communicated with the liquid inlet channel, and gas can drive the liquid in the liquid inlet channel to flow to the reaction channel through the gas channel. A detection test paper is arranged on the reaction channel. The gas channel and the liquid channel are communicated with each other, so that the sample can conveniently flow from the liquid inlet channel to the reaction channel under the driving action of the gas. The sample flow is driven in a pneumatic mode, and the operation is simple and convenient. Meanwhile, the manufacturing process of the biochemical detection device is simplified, and the cost is reduced.

Description

Technical Field

[0001] This invention relates to the field of rapid diagnostic technology, and more particularly to a biochemical detection device. Background Technology

[0002] Point-of-care testing (POCT) refers to clinical testing performed at the patient's side, enabling immediate analysis at the sampling site. This eliminates the complex processing procedures required for laboratory testing, providing rapid results. POCT products have become an important and fastest-growing segment of the in vitro diagnostic products (IDV) industry. POCT is demonstrating significant market potential and application space in rapid on-site testing in hospital operating rooms, emergency rooms, and intensive care units, as well as in the development of medical facilities in remote areas.

[0003] Unlike traditional large-scale in vitro diagnostic equipment, POCT products do not require operators to collect large numbers of samples for centralized processing. Instead, they perform diagnostics directly on individual samples at the sampling site, quickly obtaining biochemical test results. However, most existing POCT products rely on the siphon effect to achieve sample flow. The siphon effect has high requirements for the length and cross-sectional dimensions of the liquid channel, making the manufacturing process complex and cumbersome for users. In addition, traditional POCT products also struggle to measure multiple biological and chemical indicators simultaneously with a single sample collection.

[0004] Therefore, there is an urgent need to provide a biochemical detection device to solve the above problems. Summary of the Invention

[0005] The purpose of this invention is to provide a biochemical detection device that has a simple manufacturing process, is easy to operate, and can detect multiple biochemical indicators.

[0006] To achieve the above objectives, the following technical solution is provided: A biochemical detection device includes a substrate and a component disposed on the substrate: A liquid channel, comprising an inlet channel and a reaction channel that are interconnected; A calibration channel, which is connected to the reaction channel, allows calibration reagents to flow into the reaction channel through the calibration channel; A gas channel, which is connected to the liquid inlet channel, allows gas to drive the liquid in the liquid inlet channel to flow into the reaction channel via the gas channel; A test strip, wherein the reaction electrode on the test strip extends into the reaction channel.

[0007] As a preferred technical solution, it also includes a waste liquid channel disposed on the substrate, the waste liquid channel being connected to the reaction channel, and the liquid in the reaction channel being able to flow to the waste liquid channel under the drive of gas.

[0008] As a preferred technical solution, a pneumatic drive mechanism is also included, the pneumatic drive mechanism comprising: An airbag is disposed inside the substrate and connected to the gas channel, and the airbag contains gas. A driving element is used to compress the airbag so that the gas in the airbag is output through the gas channel to drive the liquid in the liquid channel.

[0009] As a preferred technical solution, the substrate has a storage cavity, the airbag is located in the storage cavity, the storage cavity has a through hole connecting to the outside, and the driving member is a thin sheet disposed between the airbag and the through hole. Pressing the thin sheet can cause the gas in the airbag to be output through the gas channel.

[0010] As a preferred technical solution, it also includes a calibration reagent storage mechanism, which includes a liquid storage tank disposed on the substrate and a calibration reagent pack disposed in the liquid storage tank. The liquid storage tank is connected to the calibration channel. The calibration reagent pack stores the calibration reagent. After the calibration reagent in the calibration reagent pack is released, it flows out through the calibration channel.

[0011] As a preferred technical solution, the bottom of the liquid storage tank is provided with a needle, and the liquid storage tank is an open groove. Pressing the calibration reagent pack causes the needle to puncture the calibration reagent pack, thereby releasing the calibration reagent.

[0012] As a preferred technical solution, the needle is a pyramidal structure, and the calibration channel extends along the side wall of the liquid storage tank to the needle.

[0013] As a preferred technical solution, both the gas channel and the liquid channel are grooves formed on the same side of the substrate, and the grooves are sealed by a cover; an inlet is formed on the cover, and the inlet is connected to the liquid inlet channel.

[0014] As a preferred technical solution, the biochemical detection device further includes a sealing mechanism, which includes a groove formed on the substrate and a slider slidably disposed in the groove. The slider extends out of the groove and is connected to a sealing block. After the slider drives the sealing block to slide to the sample inlet, the sealing block blocks the sample inlet.

[0015] As a preferred technical solution, one end of the sealing block along the sliding direction is connected to the slider, and the other end is suspended. A channel plate is protruding on each of the base plates on both sides of the groove. The middle part of the sealing block has protrusions on both sides, and each protrusion corresponds to a channel plate. The bottom height of the protrusion is less than the top height of the channel plate.

[0016] As a preferred technical solution, a first isolation step is provided between the reaction channel and the liquid inlet channel, and the height of the first isolation step is higher than the bottom surface height of the reaction channel and the liquid inlet channel.

[0017] As a preferred technical solution, the first isolation step and the bottom surface of the liquid inlet channel have a smooth transition, and the first isolation step and the bottom surface of the reaction channel also have a smooth transition.

[0018] As a preferred technical solution, a second isolation step is provided between the reaction channel and the waste liquid channel, and the height of the second isolation step is higher than the bottom surface height of the reaction channel and the waste liquid channel.

[0019] As a preferred technical solution, the second isolation step smoothly transitions to the bottom surface of the liquid inlet channel, and the second isolation step also smoothly transitions to the bottom surface of the reaction channel.

[0020] As a preferred technical solution, the test strip has multiple reaction electrodes.

[0021] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention, by setting up interconnected gas and liquid channels, allows samples to flow conveniently from the inlet channel to the reaction channel under the propulsion of gas. By using a pneumatic drive to drive the sample flow, the operation is simple and convenient. It also overcomes the strict requirements of the manufacturing process when using the siphon effect to complete the liquid flow in traditional methods, simplifying the manufacturing process of biochemical detection equipment and reducing production costs. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the structure of the biochemical detection device in an embodiment of the present invention.

[0023] Figure 2 This is a front view of the biochemical detection device in an embodiment of the present invention.

[0024] Figure 3 This is a partial structural schematic diagram of the biochemical detection device in an embodiment of the present invention.

[0025] Figure 4 This is a cross-sectional schematic diagram of the biochemical detection device in an embodiment of the present invention.

[0026] Figure 5 This is a schematic diagram of the structure of the sealing element in an embodiment of the present invention.

[0027] Figure 6 for Figure 1 A magnified view of a portion of point A in the middle.

[0028] Figure label: 100-Substrate; 101-Detection end plate; 102-Handheld end plate; 200-Liquid inlet channel; 300-Reaction channel; 400-Calibration channel; 500-Waste liquid channel; 600-Gas channel; 700-Cover; 701-Sample inlet; 800-Test strip; 1-Pneumatic drive mechanism; 11-Storage cavity; 12-Airbag; 13-Drive component; 14-Through hole; 2-First isolation step; 3-Second isolation step; 4-Calibration reagent storage mechanism; 41-Reservoir; 42-Calibration reagent pack; 43-Needle; 5-Sealing mechanism; 51-Slide groove; 52-Slider; 53-Sealing block; 531-Drive unit; 532-Sealing unit; 5321-Sealing body; 533-Protrusion; 54-Channel plate. Detailed Implementation

[0029] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. The components of the embodiments of the present invention described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0030] Therefore, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. That is, the embodiments of the invention are merely examples to clearly illustrate the invention, and are not intended to limit the implementation of the invention. For those skilled in the art, other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively describe all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the invention should be included within the scope of protection of the claims of the invention. That is, based on the embodiments of the invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of the invention.

[0031] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0032] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product of this invention is in use. These terms are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance. The terms "first position" and "second position" refer to two different positions. In the description of this invention, unless otherwise stated, "a plurality of" means two or more.

[0033] In the description of this invention, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0034] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0035] Embodiments of the present invention are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0036] like Figure 1-3As shown, this embodiment provides a biochemical detection device, including a substrate 100 and a liquid channel, a calibration channel 400, a gas channel 600, and a test strip 800 disposed on the substrate 100. The liquid channel includes an inlet channel 200 and a reaction channel 300 that are interconnected; the calibration channel 400 is connected to the reaction channel 300, and calibration reagent flows into the reaction channel 300 through the calibration channel 400; the gas channel 600 is connected to the inlet channel 200, and gas drives the sample in the inlet channel 200 to flow into the reaction channel 300 through the gas channel 600; the reaction electrode on the test strip 800 extends into the reaction channel 300. This embodiment, by setting up an interconnected gas channel 600 and a liquid channel, allows the sample to flow conveniently from the inlet channel 200 to the reaction channel 300 under the propulsion of gas. The sample flow is driven by pneumatics, making operation simple and convenient. It also overcomes the stringent manufacturing requirements of traditional methods that use a siphon effect to achieve liquid flow, simplifying the manufacturing process of biochemical detection devices. Optionally, the test strip 800 may be equipped with multiple reaction electrodes to enable the detection of multiple biochemical indicators.

[0037] The biochemical detection equipment also includes a waste liquid channel 500 disposed on the substrate 100. The waste liquid channel 500 is connected to the reaction channel 300. After the detection is completed, the liquid in the liquid inlet channel 200 and the reaction channel 300 flows to the waste liquid channel 500 under the action of gas for unified collection and treatment.

[0038] refer to Figure 4 The biochemical detection equipment also includes a pneumatic drive mechanism 1, specifically comprising an air bladder 12 and a drive member 13. The air bladder 12 is disposed inside the substrate 100 and communicates with the gas channel 600, and the air bladder 12 stores gas. The drive member 13 is used to compress the air bladder 12 so that the gas in the air bladder 12 is output through the gas channel 600 to drive the liquid in the liquid channel. Specifically, in order to compress the volume of the testing equipment, the air bladder 12 and the gas channel 600 are disposed on different sides of the substrate 100; a storage cavity 11 is opened on one side of the substrate 100 where the air bladder 12 is located, and the air bladder 12 is located in the storage cavity 11; at the same time, the storage cavity 11 has a through hole 14 communicating with the outside, and the drive member 13 is a thin sheet disposed between the air bladder 12 and the through hole 14. When gas-driven operation is required, an external driving force is applied to the sheet through the through-hole 14 to compress the airbag 12. The gas stored in the airbag 12 is pushed along the gas channel 600 to the liquid channel, driving the liquid in the liquid inlet channel 200 into the reaction channel 300. After the detection is completed, the driven liquid flows from the reaction channel 300 into the waste liquid channel 500. Optionally, the through-hole 14 is a circular structure, and correspondingly, the sheet is a circular iron sheet structure. Further optionally, the pneumatic drive mechanism 1 is located at the bottom of the substrate 100 to prevent accidental operation by personnel.

[0039] Refer again Figure 3 To ensure that the liquid inlet channel 200 and the reaction channel 300 do not interfere with each other, and to allow the test strip 800 to complete the test in a stable liquid environment, a first isolation step 2 should be provided between the reaction channel 300 and the liquid inlet channel 200. The height of the first isolation step 2 is higher than the bottom surface of both the reaction channel 300 and the liquid inlet channel 200. Liquid can only cross the first isolation step 2 and enter the reaction channel 300 when the gas bladder 12 is compressed for gas propulsion. Simultaneously, when gas propulsion stops, the liquid in the liquid inlet channel 200 is isolated by the first isolation step 2 and cannot enter the reaction channel 300, thus avoiding interference with the detection of the sample in the reaction channel 300. Furthermore, the first isolation step 2 has a smooth transition with the bottom surface of the liquid inlet channel 200 and the bottom surface of the reaction channel 300 to reduce resistance to liquid flow. In practical applications, inconsistent sample addition by personnel can lead to insufficient samples, requiring repeated sample collection, reducing detection efficiency and negatively impacting the user experience. In this embodiment, by setting up the liquid inlet channel 200 and the first isolation step 2, sufficient samples can be stored in the liquid inlet channel 200. If the flow rate of samples into the reaction channel 300 is insufficient, the pneumatic drive mechanism 1 can replenish the samples into the reaction channel 300. When the pneumatic drive mechanism 1 is stopped, the samples that failed to flow into the reaction channel can return to the liquid inlet channel 200 for storage, avoiding a situation where there are not enough samples. Further optionally, the bottom surface of the liquid inlet channel 200 is lower than the bottom surface of the reaction channel 300 to increase the capacity of the liquid inlet channel 200, allowing the liquid inlet channel 200 to store more samples.

[0040] To prevent samples being tested in the reaction channel 300 from entering the waste liquid channel 500, causing sample loss and affecting the testing process, a second isolation step 3 should be provided between the reaction channel 300 and the waste liquid channel 500. The height of the second isolation step 3 should be higher than the bottom surface height of both the reaction channel 300 and the waste liquid channel 500. After the test is completed, the air bladder 12 is compressed to drive the liquid in the reaction channel 300 across the second isolation step 3 into the waste liquid channel 500. Furthermore, the second isolation step 3 should have a smooth transition with the bottom surface of the reaction channel 300 and the bottom surface of the waste liquid channel 500 to reduce resistance to liquid flow. Optionally, the bottom surface height of the reaction channel 300 should be higher than the bottom surface height of the waste liquid channel 500 to increase the capacity of the waste liquid channel 500 and facilitate the storage of more waste liquid.

[0041] refer to Figure 1 In this embodiment, the biochemical detection device also includes a calibration reagent storage mechanism 4 for storing calibration reagents. (See reference...) Figure 2 and Figure 3The calibration reagent storage mechanism 4 includes a storage tank 41 disposed on a substrate 100 and a calibration reagent pack 42 disposed within the storage tank 41. The calibration reagent pack 42 stores calibration reagent. The storage tank 41 is connected to the calibration channel 400. When testing is required, the calibration reagent in the calibration reagent pack 42 is released and flows into the storage tank 41, and then flows into the reaction channel 300 through the calibration channel 400 to complete the calibration work. Optionally, a needle 43 is provided at the bottom of the storage tank 41. The storage tank 41 is an open groove. By pressing the calibration reagent pack 42, the needle 43 punctures the calibration reagent pack 42, thereby releasing the calibration reagent. Optionally, the needle 43 has a pyramidal structure. The calibration channel 400 can extend along the side wall of the storage tank 41 to the pyramidal structure of the needle 43, so that the calibration reagent flows along the calibration channel 400 as much as possible, avoiding flowing to other parts of the storage tank 41, thereby reducing waste. Optionally, the liquid storage tank 41 and the storage cavity 11 are respectively located on the upper and lower sides of the substrate 100, and the liquid storage tank 41, the liquid channel and the gas channel 600 are all located on the same side of the substrate 100. This not only facilitates the connection between the calibration channel 400 and the reaction channel 300, but also reduces the volume of the biochemical detection device, making the structure more compact.

[0042] In this embodiment, both the gas channel 600 and the liquid channel are grooves formed on the same side of the substrate 100. To ensure the sealing of the liquid channel and prevent external impurities from entering, refer again... Figure 1-3 The groove of the substrate 100 is sealed by a cover 700. Optionally, the cover 700 can be a thin film made of PET material, which can ensure sealing, simplify the manufacturing process of the entire device, reduce manufacturing costs, and improve production efficiency. It is foreseeable that the cover 700 only needs to cover the gas channel 600 and the liquid channel that need to be sealed. Furthermore, the cover 700 has a sample inlet 701, which is connected to the liquid inlet channel 200. The operator adds the sample into the liquid inlet channel 200 through the sample inlet 701. Optionally, one end of the gas channel 600 connected to the liquid inlet channel 200 can be located near the sample inlet 701 to fully deliver the sample into the reaction channel 300 and reduce sample waste. Furthermore, the cover 700 can optionally cover the outer edge of the liquid storage tank 41, which can both avoid interfering with external pressure on the calibration reagent pack 42 and isolate the liquid storage tank 41 from the outside environment as much as possible.

[0043] The sample inlet 701 is directly mounted on the cover 700. While this facilitates sample addition, the exposed inlet 701 inevitably comes into contact with the outside environment after sample addition, potentially contaminating the sample and affecting the accuracy of the final detection results. Therefore, the biochemical detection device in this embodiment also includes a sealing mechanism 5, as shown in the reference... Figure 1-3The sealing mechanism 5 includes a groove 51 formed on the substrate 100 and a sealing element slidably disposed in the groove 51. After the sample is added, the sealing element slides within the groove 51 to the sample inlet 701, thus sealing the sample inlet 701. Further, refer to... Figure 5 The sealing element includes a slider 52 and a sealing block 53 connected from bottom to top. The slider 52 is slidably connected to the groove 51. After extending from the groove 51, the slider 52 connects to the sealing block 53, which is used to seal the sample inlet 701. Through the above-mentioned sliding sealing design, the sealing mechanism 5 is reliably assembled with the substrate 100, facilitating personnel to complete the sealing operation. At the same time, the slider 52 is hidden within the substrate 100 as much as possible, with only the sealing block 53 exposed, which also simplifies the structural design, reduces the size of the detection equipment, and is conducive to the development of products towards miniaturization and compactness. Optionally, the groove 51 is a T-shaped groove provided on the substrate 100. Correspondingly, the slider 52 is also a T-shaped structure. The sliding fit of the T-shaped structure can prevent the slider 52 from deviating during sliding, thus making it slide only in a specific direction.

[0044] Optionally, refer again Figure 5 The sealing block 53 includes a driving part 531 and a sealing part 532 arranged along the sliding direction. The driving part 531 is designed with a wedge-shaped surface structure, which facilitates the operation of the sealing block 53 by hand. The sealing part 532 is used to directly contact the sample inlet 701, thereby sealing the sample inlet 701. Optionally, anti-slip texture is provided on the wedge-shaped surface to improve the friction during driving. Optionally, in this embodiment, the sample inlet 701 is a circular through hole 14, and the bottom of the sealing part 532 is provided with a spherical sealing body 5321 to fully seal the sample inlet 701. In this embodiment, the sealing body 5321 is a hemispherical structure, and the radius of the hemispherical structure is larger than the radius of the sample inlet 701, so that the sealing body 5321 can be embedded in the sample inlet 701 to achieve the sealing purpose. In specific implementation, the sealing body 5321 is not limited to a hemispherical structure, as long as it can completely seal the sample inlet 701. Furthermore, since the injection port 701 is located on the thin film material, and the thin film material has a certain degree of extensibility, when the hemispherical structure of the sealing body 5321 is embedded in the injection port 701, it will inevitably bring the thin film material near the injection port 701 into the injection port 701, causing it to extend to a certain extent, thereby improving the sealing effect of the sealing body 5321.

[0045] Further, refer to Figure 5 and Figure 6A channel plate 54 is protruding on each of the substrates 100 on both sides of the slide groove 51. The slider 52 is connected to one end of the sealing block 53 with a driving part 531, while the sealing part 532 is suspended. There are protrusions 533 on both sides of the middle part of the sealing block 53. Each protrusion 533 corresponds to a channel plate 54, and the bottom height of the protrusion 533 is less than the top height of the channel plate 54. Therefore, after the protrusion 533 slides onto the channel plate 54, one end of the sealing part 532 on the sealing block 53 is lifted up, so that the sealing body 5321 and the substrate 100 maintain a certain gap. When the sealing body 5321 slides to the injection port 701, the protrusion 533 disengages from the channel plate 54, and the sealing part 532 falls back under its own elasticity. The sealing body 5321 is just embedded in the injection port 701 to complete the sealing. In practice, after the protrusion 533 disengages from the channel plate 54 and the sealing body 5321 is embedded in the injection port 701, personnel can feel the insertion of the sealing body 5321 to determine whether the sealing is complete. Specifically, when the protrusion 533 slides onto the channel plate 54, the sealing body 5321 is located between the two channel plates 54 to avoid interference between the sealing body 5321 and the channel plate 54. Furthermore, the channel plate 54 is designed close to the injection port 701 to reduce the length of the channel plate 54. It is only necessary to ensure that the sealing part 532 can be lifted before reaching the injection port 701 and can fall back when reaching the injection port 701. Furthermore, the end of the channel plate 54 away from the injection port 701 has a smooth transition with the substrate 100 so that the protrusion 533 can slide smoothly onto the channel plate 54.

[0046] In this embodiment, optionally, the substrate 100 adopts a rectangular structure, and the liquid channel is arranged along the side of the rectangular structure. The inlet channel 200 and the waste liquid channel 500 are respectively located on the two sides of the substrate 100 along its width direction, and the sealing mechanism 5 and the reaction channel 300 are respectively located on the two sides of the substrate 100 along its length direction. The reaction channel 300 and the chute 51 are both perpendicular to the inlet channel and the waste liquid channel 500. Further, refer to... Figure 3 A detection end plate 101 is provided at one end of the substrate 100 along its length. A slot is provided on the detection end plate 101, through which a test strip 800 is inserted. The end of the test strip 800 with the reaction electrode extends into the reaction channel 300, while the working electrode is located on the detection end plate 101, so that it can be placed into a diagnostic device to complete the diagnosis. Furthermore, a handheld end plate 102 is provided at the other end of the substrate 100 along its length, so that the operator can hold the entire biochemical detection device. Optionally, the handheld end plate 102 is provided with anti-slip texture to improve the stability of the operator's grip.

[0047] In this embodiment, the test strip 800 is equipped with reaction electrodes for multiple biochemical indicators, enabling simultaneous detection of multiple indicators and improving detection efficiency. Optionally, the test strip 800 uses screen printing technology to print the reaction electrodes onto the corresponding substrate. Applying screen printing technology to the POCT field simplifies the manufacturing process of the reaction electrodes and reduces manufacturing costs. Furthermore, the substrate of the test strip 800 is made of PVC or PET; the conductive layer of the test strip 800 is screen-printed using Ag, AgCl, or C raw materials, and an insulating oil is brushed onto it as an insulating layer; the working electrode of the test strip 800 is covered with an ion-selective membrane, and the reference electrode is covered with an electrolyte membrane and a multi-component polymer mixture.

[0048] In summary, the biochemical testing equipment provided in this embodiment, when used in conjunction with diagnostic equipment, can be used in various medical scenarios to achieve rapid testing in places such as hospital operating rooms, emergency rooms, or intensive care units, meeting the needs of efficient and fast-paced work styles.

[0049] Specifically, the specific steps for performing the detection using the biochemical detection equipment provided in this embodiment include: 1) Add the sample to the injection port 701 using a pipette or syringe; 2) Push the sealing element until you feel the sealing body 5321 being inserted into the injection port 701, then the sealing is complete; 3) Insert the detection end plate 101 of the biochemical detection equipment into the diagnostic equipment; 4) The diagnostic equipment is equipped with a corresponding mechanism to press down the calibration reagent pack 42, thereby puncturing the calibration reagent pack 42 and releasing the calibration reagent. The calibration reagent flows into the reaction channel 300 to complete the calibration work. 5) After calibration, the diagnostic equipment is also equipped with a corresponding mechanism to press the drive component 13 and the airbag 12, so that the sample is pushed to the reaction channel 300, and at the same time the calibration reagent is pushed to the waste liquid channel 500; the diagnostic equipment performs diagnostic testing on the sample in the reaction channel 300.

[0050] Note that the above description is merely a preferred embodiment of the present invention and the technical principles employed. Those skilled in the art will understand that the present invention is not limited to the specific embodiments described herein, and various obvious changes, readjustments, and substitutions can be made without departing from the scope of protection of the present invention. Therefore, although the present invention has been described in detail through the above embodiments, the present invention is not limited to the above embodiments, and may include many other equivalent embodiments without departing from the concept of the present invention, the scope of which is determined by the scope of the appended claims.

Claims

1. A biochemical detection device, characterized in that, The system includes a substrate (100) and the following disposed on the substrate (100): a liquid channel, the liquid channel including an inlet channel (200) and a reaction channel (300) that are interconnected; a calibration channel (400) that is connected to the reaction channel (300), through which calibration reagent can flow into the reaction channel (300); a gas channel (600) that is connected to the inlet channel (200), through which gas can drive the liquid in the inlet channel (200) to flow into the reaction channel (300); and a test strip (800) on which a reaction electrode extends into the reaction channel (300).

2. The biochemical detection device according to claim 1, characterized in that, It also includes a waste liquid channel (500) disposed on the substrate (100), the waste liquid channel (500) being connected to the reaction channel (300), and the liquid in the reaction channel (300) being able to flow to the waste liquid channel (500) under the drive of gas.

3. The biochemical detection device according to claim 1 or 2, characterized in that, It also includes a pneumatic drive mechanism (1), which includes: an airbag (12) disposed inside the substrate (100) and connected to the gas channel (600), the airbag (12) storing gas; and a drive member (13) for pressing the airbag (12) so that the gas in the airbag (12) is output from the gas channel (600) to drive the liquid in the liquid channel. The substrate (100) has a storage cavity (11) and the airbag (12) is located in the storage cavity (11). The storage cavity (11) has a through hole (14) connecting to the outside. The drive member (13) is a thin sheet disposed between the airbag (12) and the through hole (14). Pressing the thin sheet can cause the gas in the airbag (12) to be output from the gas channel (600).

4. The biochemical detection device according to claim 1, characterized in that, It also includes a calibration reagent storage mechanism (4), which includes a liquid storage tank (41) disposed on the substrate (100) and a calibration reagent pack (42) disposed in the liquid storage tank (41). The liquid storage tank (41) is connected to the calibration channel (400). The calibration reagent pack (42) stores the calibration reagent. After the calibration reagent in the calibration reagent pack (42) is released, it flows out from the calibration channel (400). The bottom of the liquid storage tank (41) is provided with a needle (43). The liquid storage tank (41) is an open slot. Pressing the calibration reagent pack (42) causes the needle (43) to puncture the calibration reagent pack (42) and release the calibration reagent.

5. The biochemical detection device according to claim 6, characterized in that, The needle (43) has a pyramidal structure, and the calibration channel (400) extends along the side wall of the liquid storage tank (41) to the needle (43).

6. The biochemical detection device according to claim 1, characterized in that, Both the gas channel (600) and the liquid channel are grooves formed on the same side of the substrate (100), and the grooves are sealed by a cover (700); an inlet (701) is formed on the cover (700), and the inlet (701) is connected to the liquid inlet channel (200).

7. The biochemical detection device according to claim 8, characterized in that, The biochemical detection device also includes a sealing mechanism (5), which includes a groove (51) opened on the substrate (100) and a slider (52) slidably disposed in the groove (51). The slider (52) extends out of the groove (51) and is connected to a sealing block (53). After the slider (52) drives the sealing block (53) to slide to the injection port (701), the sealing block (53) blocks the injection port (701).

8. The biochemical detection device according to claim 9, characterized in that, One end of the sealing block (53) along the sliding direction is connected to the slider (52), and the other end is suspended. A channel plate (54) is protruding on each of the base plates (100) on both sides of the slide groove (51). The middle part of the sealing block (53) is provided with protrusions (533) on both sides. Each protrusion (533) corresponds to a channel plate (54), and the bottom height of the protrusion (533) is less than the top height of the channel plate (54).

9. The biochemical detection device according to claim 1, characterized in that, A first isolation step (2) is provided between the reaction channel (300) and the liquid inlet channel (200). The height of the first isolation step (2) is higher than the bottom surface height of the reaction channel (300) and the liquid inlet channel (200). The first isolation step (2) and the bottom surface of the liquid inlet channel (200) are smoothly connected. The first isolation step (2) and the bottom surface of the reaction channel (300) are also smoothly connected.

10. The biochemical detection device according to claim 2, characterized in that, A second isolation step (3) is provided between the reaction channel (300) and the waste liquid channel (500). The height of the second isolation step (3) is higher than the bottom surface height of the reaction channel (300) and the waste liquid channel (500). The second isolation step (3) and the bottom surface of the liquid inlet channel (200) are smoothly connected. The second isolation step (3) and the bottom surface of the reaction channel (300) are also smoothly connected.

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