A gene detection method and gene detection cartridge based on magnetic control technology

By setting up a capillary magnetic bead movement channel and a sample processing chamber within the molecular POCT detection cartridge, and combining magnetic control technology with isolation of high-viscosity liquid substances, the problems of isolation and driving complexity of functional liquids are solved, costs are reduced, product yield is improved, and simplified molecular POCT detection is achieved.

CN122128086APending Publication Date: 2026-06-02HANGZHOU TIANWEI GENE TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HANGZHOU TIANWEI GENE TECH CO LTD
Filing Date
2026-01-31
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

In existing molecular POCT technologies, the isolation and sealing of functional liquids within closed test cartridges is complex, the liquid-driven design is complex and costly, residual air bubbles affect test results, and the product defect rate is high, which limits its widespread application.

Method used

By employing capillary liquid isolation technology and magnetically controlled nucleic acid transfer technology, capillary magnetic bead movement channels, sample processing chambers, and amplification chambers are set up in the detection cartridge. High-viscosity liquid substances or thermoplastic hydrophobic substances are used to isolate functional areas, and nucleic acid transfer is controlled by magnetic field. The problem of air bubbles is solved by combining annular fluid channels and U-shaped fluid channels, simplifying equipment design and improving yield.

Benefits of technology

This design simplifies the design of the testing cartridges and equipment, reduces production costs, improves product yield, and ensures the reliability of the sealed preservation and testing of functional liquids.

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Abstract

This invention discloses a gene detection method and gene detection cartridge based on magnetic control technology, comprising: setting a capillary magnetic bead moving channel, a sample processing chamber, and an amplification chamber within the detection cartridge; the sample processing chamber and the amplification chamber are separated into one or more cleaning zones by a high-viscosity liquid substance or a thermoplastic hydrophobic substance through the capillary magnetic bead moving channel; adding the sample to the sample processing chamber, mixing the sample with sample processing reagents and nano-magnetic beads, adsorbing nucleic acids by the nano-magnetic beads, and having an external magnet adsorb the nano-magnetic beads and drive them along the capillary magnetic bead moving channel sequentially through the cleaning zones into the amplification chamber; eluting the nucleic acids in the amplification chamber and performing an amplification reaction; and detecting the amplified fluorescence signal through an external optical device to achieve integrated nucleic acid extraction, amplification, and detection. This invention improves the integration of the detection cartridge, reduces manufacturing costs, and increases product yield through optimized design of capillary liquid isolation and flow channel degassing technology.
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