A cyanidin targeted controlled-release enteric capsule based on purple rice extract and a preparation method thereof

Abstract

This invention discloses a targeted controlled-release enteric-coated capsule based on purple rice extract and its preparation method, belonging to the fields of biomedicine and nutritional health care technology. The targeted controlled-release enteric-coated capsule based on purple rice extract includes a capsule shell and contents; the contents, by weight percentage, contain the following components: 28%-62% targeted modified anthocyanins, 15%-25% purple rice dietary fiber, 10%-20% multivitamin complex, 8%-15% rice milk powder, and 5%-12% protein peptides. The capsule shell uses a specific enteric-coating material. The targeted modified anthocyanins of this invention can achieve targeted controlled release in vivo, improving bioavailability. Multiple components in the purple rice extract synergistically exert multi-dimensional health care effects, including antioxidant, intestinal regulation, and nutritional supplementation. The preparation method of this invention is scientifically sound and provides a new approach for developing nutritional products with highly effective health care functions.

Description

Technical Field

[0001] This invention belongs to the fields of biomedicine and nutritional health care technology, specifically relating to an anthocyanin-targeted controlled-release enteric-coated capsule based on purple rice extract and its preparation method. Background Technology

[0002] Anthocyanins, as a natural functional ingredient, possess significant antioxidant and anti-inflammatory activities, offering numerous health benefits. However, due to the acidic environment of the stomach, anthocyanins are easily degraded after entering the body, resulting in low bioavailability and significantly limiting their health benefits.

[0003] Purple rice, a special type of rice rich in various nutrients, contains not only anthocyanins but also abundant dietary fiber, gamma-aminobutyric acid (GABA), polyphenols, flavonoids, vitamins, and minerals. Each of these components possesses unique health benefits; for example, GABA regulates nerves and lowers blood pressure; polyphenols and flavonoids have antioxidant and antibacterial effects; and vitamins participate in human metabolism and maintain normal physiological functions. However, current research on the synergistic effects of the various components in purple rice extract is insufficient, and there is a lack of a product form that can effectively integrate these components, allowing them to exert their precise effects in the body.

[0004] Most existing nutritional supplements suffer from problems such as limited ingredient variety and unreasonable release mechanisms, failing to fully meet the body's needs for diverse nutrients and the requirement for precise regulation of nutrient release. Therefore, developing a multi-dimensional health product that can achieve targeted and controlled release of anthocyanins in vivo while leveraging the synergistic health benefits of multiple components in purple rice extract is of significant practical importance. Summary of the Invention

[0005] This invention aims to provide an anthocyanin-targeted controlled-release enteric-coated capsule based on purple rice extract. Through targeted modification of anthocyanins and rational formulation design, the anthocyanins are precisely released in specific parts of the intestine, protecting them from destruction by gastric juice. At the same time, it fully leverages the synergistic health benefits of multiple components in purple rice extract, providing the human body with multi-dimensional health care effects such as anti-oxidation, intestinal regulation, and nutritional supplementation, in order to meet people's growing health needs.

[0006] Meanwhile, this invention provides a method for preparing anthocyanin-targeted controlled-release enteric-coated capsules based on purple rice extract.

[0007] To achieve the above objectives, the present invention provides the following technical solution: Composition of the enteric-coated capsules for targeted controlled release and multidimensional nourishment of anthocyanins based on purple rice extract: The enteric-coated capsules of the present invention consist of a capsule shell and contents. The contents contain the following components by weight percentage: 28%-62% targeted modified anthocyanins, 15%-25% purple rice dietary fiber, 10%-20% multidimensional complex, 8%-15% rice milk powder, and 5%-12% protein peptides. The multidimensional complex is obtained from the fermentation of purple rice germ, and the anthocyanins, dietary fiber, rice milk powder, and protein peptides are all isolated from purple rice.

[0008] Targeted modification of anthocyanins: Anthocyanins isolated from purple rice extract are covalently linked or physically encapsulated with a targeting carrier under specific conditions. The targeting carrier is a biodegradable polymer material with targeting specific sites in the intestine, such as one or more combinations of chitosan, sodium alginate, or polylactic-co-glycolic acid copolymer (PLGA). Specific conditions include a reaction temperature of 25℃-35℃, a reaction time of 4-8 hours, and a pH of 6.0-7.0. This targeted modification enables the precise release of anthocyanins at specific sites in the intestine, improving their bioavailability.

[0009] Purple rice dietary fiber: Obtained from purple rice extract using physical separation and chemical purification methods. Through screening, filtration, and ion exchange, impurities and other components are removed to obtain purple rice dietary fiber with a dietary fiber content of no less than 90% and an average molecular weight of no less than 5000 Da. In the intestines, it can absorb water, increase stool volume, promote intestinal peristalsis, regulate the balance of intestinal flora, and slow down the digestion and absorption of carbohydrates, helping to maintain stable blood sugar and blood lipid levels.

[0010] Multivitamin Complex: This is a prebiotic complex derived from fermented purple rice germ rice, containing at least three of the following: gamma-aminobutyric acid (GABA), polyphenols, flavonoids, vitamin A, vitamin C, vitamin E, and B vitamins. The various components are blended in optimal proportions to meet human health needs. GABA helps regulate nervous system function and improve sleep; polyphenols and flavonoids synergistically enhance antioxidant capacity with anthocyanins; vitamin A is beneficial for vision and the immune system; vitamins C and E are antioxidants; and B vitamins participate in energy metabolism, ensuring the normal functioning of the body.

[0011] Protein peptides are prepared through enzymatic hydrolysis or chemical synthesis, with an average molecular weight between 800-2000 Da. They are composed of at least 8 different amino acids, of which at least 40% are essential amino acids for the human body. Protein peptides have good solubility and absorption, and can quickly replenish the amino acids needed by the human body, promote protein synthesis, and enhance immunity.

[0012] Rice milk powder: As a nutritional supplement, it is rich in a variety of nutrients, such as protein, carbohydrates, and minerals, which can provide the human body with necessary energy and nutritional support, while improving the taste and stability of the product.

[0013] Capsule shell: Made of enteric-coated materials, such as hydroxypropyl methylcellulose phthalate, cellulose acetate phthalate, polyacrylic acid resin II and III, or a mixture thereof. These enteric-coated materials do not dissolve in the acidic environment of the stomach, protecting the contents from being destroyed by gastric juices, but dissolve rapidly in the alkaline environment of the intestine, releasing the contents.

[0014] Preparation method of anthocyanin in vivo targeted controlled release and multidimensional nourishing enteric-coated capsules based on purple rice extract: Preparation of Purple Rice Extract: Purple rice was pulverized to achieve a specific particle size, and then degreased using an organic solvent to remove oils and other impurities. Next, extraction was performed using a water-ethanol mixed solvent. By controlling the extraction temperature, time, and solvent ratio, the effective components of the purple rice were fully dissolved in the solvent. After filtration to remove insoluble impurities, the extract was obtained through concentration and drying.

[0015] Isolation and targeted modification of anthocyanins: High-purity anthocyanins were separated from purple rice extract using chromatographic techniques such as high-performance liquid chromatography (HPLC) or column chromatography. The anthocyanins were then reacted with a targeting carrier under specific conditions to obtain targeted-modified anthocyanins.

[0016] Preparation of purple rice dietary fiber: Crude dietary fiber is initially separated from purple rice extract using physical methods such as centrifugation and filtration. Then, impurities and other components are removed through chemical purification methods such as acid-base treatment and ion exchange to obtain purple rice dietary fiber that meets the requirements.

[0017] Preparation of the multidimensional complex: Purple rice germ rice was fermented at a temperature of 30℃-37℃ for 48-72 hours. The fermentation strain was a mixture of lactic acid bacteria (commercially available freeze-dried lactic acid bacteria powder) and yeast (commercially available freeze-dried yeast powder) (the mass ratio of lactic acid bacteria to yeast was 1:1, and the amount of the mixed strain added was 3wt% of the fermentation liquid). After fermentation, the multidimensional complex was obtained through steps such as filtration, concentration, and drying.

[0018] Preparation of protein peptides: Enzymatic hydrolysis can be used, in which a suitable protease is selected to hydrolyze the protein raw material, and the hydrolysis conditions are controlled to obtain protein peptides within the required molecular weight range; alternatively, chemical synthesis can be used to synthesize them according to the designed amino acid sequence.

[0019] Contents mixing: The targeted modified anthocyanins, purple rice dietary fiber, multivitamin complex, rice milk powder, and protein peptides are added to the mixing equipment according to the specified weight percentages and mixed evenly at a certain stirring speed and time to ensure that each component is fully dispersed.

[0020] Capsule filling: Using capsule filling equipment, the mixed contents are accurately filled into the outer shell of enteric-coated capsules. After quality testing, the enteric-coated capsules with in vivo targeted controlled release and multi-dimensional care of anthocyanins based on purple rice extract are obtained.

[0021] Specifically, a method for preparing anthocyanin-targeted controlled-release enteric-coated capsules based on purple rice extract includes the following steps: Step 1, Preparation of Purple Rice Extract: Take purple rice, pulverize it to 20 mesh, defatt it three times with petroleum ether at 40℃, 2 hours each time. Then add 8 times the volume of 70% ethanol-water solution, extract at 60℃ for 3 hours, filter, concentrate the filtrate to 1 / 5 of the original volume, and then freeze-dry to obtain purple rice extract.

[0022] Step 2, Isolation and Targeted Modification of Anthocyanins: Anthocyanins were isolated from the purple rice extract using preparative high-performance liquid chromatography (HPLC). The anthocyanins were reacted with a targeting carrier at 25-35℃ and pH 6.0-7.0 for 4-8 hours, resulting in targeted-modified anthocyanins through physical encapsulation. The mass ratio of anthocyanins to the targeting carrier was (4-15):1.

[0023] The targeting carrier includes one or more combinations of chitosan, sodium alginate, or polylactic-co-glycolic acid copolymer (PLGA).

[0024] Step 3, Preparation of Purple Rice Dietary Fiber: The crude dietary fiber is initially separated from the purple rice extract by physical methods such as centrifugation and filtration. Then, it is treated with 0.5% sodium hydroxide solution at 50°C for 2 hours, and then impurities are removed with ion exchange resin to obtain purple rice dietary fiber with a dietary fiber content of not less than 90% and an average molecular weight of not less than 5000 Da.

[0025] Step 4, preparation of the multidimensional complex: Purple rice germ rice is fermented at a temperature of 30-37℃ for 48-72 hours. The fermentation strain is a mixture of lactic acid bacteria and yeast. After fermentation, the multidimensional complex is obtained through filtration, concentration, and drying.

[0026] Step 5, Preparation of protein peptides: Rice protein is enzymatically hydrolyzed using a variety of proteases (including at least trypsin and V8 protease), and the hydrolysis conditions are controlled to obtain protein peptides with an average molecular weight of 800-2000 Da. The amino acid composition meets the requirements, and the content of essential amino acids for the human body is not less than 40%.

[0027] Step 6, Mixing the contents: Add the targeted modified anthocyanins, purple rice dietary fiber, multi-dimensional complex, rice milk powder, and protein peptides to a three-dimensional mixer according to the mass percentage, and stir at 50 r / min for 30 minutes to obtain a homogeneous mixture of contents.

[0028] Step 7, Capsule filling: Using a capsule filling machine, the above-mentioned mixture of contents is filled into the capsule shell of the enteric material. Each capsule is filled with 500mg of contents, and a total of 1000 enteric-coated capsules based on purple rice extract for in vivo targeted controlled release and multi-dimensional maintenance of anthocyanins are prepared.

[0029] Capsule shell: made of enteric material, such as hydroxypropyl methylcellulose phthalate, cellulose acetate phthalate, polyacrylic acid resin II and III or a mixture thereof.

[0030] The enteric-coated capsules of the present invention were placed in simulated gastric juice and simulated intestinal juice in sequence for in vitro release simulation experiments. It was found that the cumulative release rate of anthocyanins in simulated gastric juice was less than 10% in 1 hour and higher than 60% in simulated intestinal juice in 3 hours. This indicates that the enteric-coated capsules can effectively protect anthocyanins from degradation by gastric acid in vivo and achieve targeted controlled release in the intestine.

[0031] The present invention relates to the application of anthocyanin-targeted controlled-release enteric-coated capsules based on purple rice extract in health supplements.

[0032] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention achieves precise release of anthocyanins at specific sites in the intestine through targeted modification of anthocyanins, effectively protecting anthocyanins from destruction by gastric juices, greatly improving the bioavailability of anthocyanins, and enhancing their antioxidant and other health benefits.

[0033] The multiple components in purple rice extract work synergistically to provide multidimensional health benefits. The antioxidant effects of anthocyanins, polyphenols, and flavonoids work together to more effectively eliminate free radicals in the body and delay aging; purple rice dietary fiber regulates gut microbiota and promotes intestinal health; components such as γ-aminobutyric acid in the multidimensional complex regulate the nervous system and other functions, while vitamins supplement the body's nutritional needs; protein peptides and rice milk powder provide nutritional support and enhance immunity. This synergistic approach of multiple components provides comprehensive and integrated health benefits for the human body.

[0034] The enteric-coated capsule design of this invention ensures the stability of the contents in the acidic environment of the stomach, avoids the damage of the ingredients by the stomach acid, and enables the product to be released and exert its effects safely and effectively in the intestine, thereby improving the reliability and safety of the product.

[0035] The preparation method of this invention is scientific and reasonable, with clear operating conditions and quality control standards for each step, which is conducive to industrial production and provides a feasible technical solution for developing nutritional products with high-efficiency health care functions.

[0036] This invention discloses a targeted controlled-release enteric-coated capsule based on purple rice extract and its preparation method. The contents of the enteric-coated capsule mainly consist of targeted modified anthocyanins, purple rice dietary fiber, a multidimensional complex obtained from the fermentation of purple rice germ, rice milk powder, and protein peptides. The capsule shell uses a specific enteric-coating material. The targeted modified anthocyanins can achieve targeted controlled release in vivo, improving bioavailability. Multiple components in the purple rice extract synergistically exert multidimensional health benefits, including antioxidant effects, intestinal regulation, and nutritional supplementation. The preparation method of this invention is scientifically sound and provides a new approach for developing nutritional products with highly effective health benefits. Detailed Implementation

[0037] The present invention will be further described in detail below with reference to specific embodiments. The following embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Example 1

[0038] This embodiment describes a preparation technology for anthocyanin-targeted controlled-release enteric-coated capsules based on purple rice extract, specifically including the following steps: I. Preparation of Purple Rice Extract: Take 1000g of purple rice, pulverize it to 20 mesh, defatt it three times with petroleum ether at 40℃ for 2 hours each time. Then add 8 times the volume of 70% ethanol-water solution, extract at 60℃ for 3 hours, filter, concentrate the filtrate to 1 / 5 of the original volume, and then freeze-dry to obtain 150g of purple rice extract.

[0039] II. Isolation and Targeted Modification of Anthocyanins: 30g of anthocyanins were isolated from rice extract using high-performance liquid chromatography (HPLC). 20g of anthocyanins were reacted with 5g of chitosan at 30℃ and pH = 6.5 for 6 hours, and the anthocyanins were obtained through physical encapsulation.

[0040] III. Preparation of Purple Rice Dietary Fiber: Crude dietary fiber was initially separated from purple rice extract by physical methods such as centrifugation and filtration. Then, it was treated with 0.5% sodium hydroxide solution at 50°C for 2 hours, and impurities were removed with ion exchange resin to obtain 25g of purple rice dietary fiber with a dietary fiber content of 92% and an average molecular weight of 6000 Da.

[0041] IV. Preparation of Multidimensional Complex: 500g of purple rice germ rice was fermented at 35℃ for 60 hours. The fermentation strain was a mixture of lactic acid bacteria and yeast. After fermentation, 50g of multidimensional complex was obtained through filtration, concentration and drying.

[0042] V. Preparation of protein peptides: Purple rice protein was enzymatically hydrolyzed using a variety of proteases. Under controlled hydrolysis conditions, 10g of protein peptides with an average molecular weight of 1500 Da were obtained. The amino acid composition met the requirements, and the content of essential amino acids for the human body was 45%.

[0043] 6. Mixing the contents: Add 30g of targeted modified anthocyanins, 25g of purple rice dietary fiber, 10g of multivitamin complex, 10g of rice milk powder, and 10g of protein peptides to a three-dimensional mixer and stir at 50r / min for 30 minutes to obtain a homogeneous mixture of contents.

[0044] VII. Capsule Filling: The above-mentioned mixture of contents was filled into the capsule shell of a capsule with hydroxypropyl methylcellulose phthalate as the enteric material using a capsule filling machine. Each capsule was filled with 500mg of contents, and a total of 1000 enteric-coated capsules based on purple rice extract for in vivo targeted release and multi-dimensional maintenance of anthocyanins were prepared.

[0045] Enteric-coated capsules were placed sequentially in simulated gastric and intestinal fluids for in vitro release experiments. The results showed that the cumulative release rate of anthocyanins in simulated gastric fluid was less than 10% after 1 hour, while the cumulative release rate in simulated intestinal fluid was more than 60% after 3 hours. This indicates that enteric-coated capsules can effectively protect anthocyanins from degradation by gastric acid in vivo and achieve targeted and controlled release in the intestines.

[0046] This embodiment describes the application of anthocyanin-targeted controlled-release enteric-coated capsules based on purple rice extract in health supplements. Example 2

[0047] This embodiment differs from Example 1 in that sodium alginate is used as an artificial template for targeted modification of anthocyanins in step two. 25g of anthocyanins and 4g of sodium alginate are reacted at 32℃ and pH = 6.3 for 7 hours, obtaining targeted-modified anthocyanins through covalent linkage. The purple rice dietary fiber obtained in step three has a dietary fiber content of 90% and an average molecular weight of 5000 Da. Step four involves fermenting the purple rice germ at 30℃ for 48 hours. Step five involves enzymatic hydrolysis to obtain protein peptides with an average molecular weight of 800 Da and an essential amino acid content of 40%. Step six involves mixing the contents, which, by weight percentage, contain the following components: 28% targeted-modified anthocyanins, 25% purple rice dietary fiber, 20% multivitamin complex, 15% rice milk powder, and 12% protein peptides. Step seven uses cellulose acetate phthalate as the enteric coating material. The remaining steps are the same as in Example 1. Enteric-coated capsules were placed sequentially in simulated gastric and intestinal fluids for in vitro release experiments. The results showed that the cumulative release rate of anthocyanins in simulated gastric fluid was less than 10% after 1 hour, while the cumulative release rate in simulated intestinal fluid was more than 60% after 3 hours. This indicates that enteric-coated capsules can effectively protect anthocyanins from degradation by gastric acid in vivo and achieve targeted and controlled release in the intestines. Example 3

[0048] This embodiment differs from Example 1 in that PLGA is used as an artificial template for targeted modification of anthocyanins in step two. 40g of anthocyanins and 8g of PLGA are reacted at 35℃ and pH = 6.0 for 4 hours, and the targeted-modified anthocyanins are obtained through physical encapsulation. Step four involves fermentation of purple rice germ at 37℃ for 72 hours. Step five involves enzymatic hydrolysis to obtain protein peptides with an average molecular weight of 2000 Da and an essential amino acid content of 50%. Step six involves mixing the contents, which, by weight percentage, contain the following components: 62% targeted-modified anthocyanins, 15% purple rice dietary fiber, 10% multivitamin complex, 8% rice milk powder, and 5% protein peptides. Step seven uses a mixture of hydroxypropyl methylcellulose phthalate and cellulose acetate phthalate as the enteric coating material. The remaining steps are the same as in Example 1. Enteric-coated capsules were placed sequentially in simulated gastric and intestinal fluids for in vitro release experiments. The results showed that the cumulative release rate of anthocyanins in simulated gastric fluid was less than 10% after 1 hour, while the cumulative release rate in simulated intestinal fluid was more than 60% after 3 hours. This indicates that enteric-coated capsules can effectively protect anthocyanins from degradation by gastric acid in vivo and achieve targeted and controlled release in the intestines. Example 4

[0049] The difference between this embodiment and Example 1 is that step two uses a chitosan + sodium alginate complex as an artificial template for targeted modification of anthocyanins. 45g of anthocyanins, 4g of chitosan, and 3g of sodium alginate were reacted at 25°C and pH 7.0 for 8 hours, obtaining the targeted-modified anthocyanins through a combination of covalent linkage and physical encapsulation. Enteric-coated capsules were then placed in simulated gastric and intestinal fluids for in vitro release experiments. The results showed that the cumulative release rate of anthocyanins in simulated gastric fluid was less than 10% after 1 hour, while the cumulative release rate in simulated intestinal fluid was greater than 60% after 3 hours. This indicates that enteric-coated capsules can effectively protect anthocyanins from degradation by gastric acid in vivo, achieving targeted and controlled release in the intestines. Example 5

[0050] The difference between this embodiment and Example 1 is that in step two, a chitosan + PLGA complex is used as an artificial template for targeted modification of anthocyanins. Specifically, 55g of anthocyanins, 6g of chitosan, and 3g of PLGA are reacted at 27°C and pH 6.1 for 5 hours to form a complex using an ionic cross-linking method. Enteric-coated capsules were then placed in simulated gastric and intestinal fluids for in vitro release experiments. The results showed that the cumulative release rate of anthocyanins in simulated gastric fluid was less than 10% after 1 hour, while the cumulative release rate in simulated intestinal fluid was greater than 60% after 3 hours. This indicates that enteric-coated capsules can effectively protect anthocyanins from degradation by gastric acid in vivo, achieving targeted and controlled release in the intestines. Example 6

[0051] The difference between this embodiment and Example 1 is that step two uses a sodium alginate + PLGA complex as an artificial template for targeted modification of anthocyanins. Specific process parameters are as follows: 60g of anthocyanins and 4g of sodium alginate solution are electrostatically adsorbed at pH 6.8 to form primary microcapsules. These microcapsules are then immersed in a 5% PLGA-dichloromethane solution, and a gradient coating structure is formed through phase separation. Enteric-coated capsules were sequentially placed in simulated gastric and intestinal fluids for in vitro release experiments. The results showed that the cumulative release rate of anthocyanins in simulated gastric fluid was less than 10% after 1 hour, while the cumulative release rate in simulated intestinal fluid was greater than 60% after 3 hours. This indicates that enteric-coated capsules can effectively protect anthocyanins from degradation by gastric acid in vivo, achieving targeted and controlled release in the intestines. Example 7

[0052] The difference between this embodiment and Example 1 is that in step two, a chitosan + sodium alginate + PLGA complex is used as an artificial template for targeted modification of anthocyanins. Enteric-coated capsules were placed in simulated gastric and intestinal fluids for in vitro release experiments. The results showed that the cumulative release rate of anthocyanins in simulated gastric fluid was less than 10% after 1 hour, while the cumulative release rate in simulated intestinal fluid was higher than 60% after 6 hours. This indicates that enteric-coated capsules can effectively protect anthocyanins from degradation by gastric acid in vivo, achieving targeted and controlled release in the intestines.

[0053] Comparative Example 1

[0054] The difference between this comparative example and Example 1 is that no targeted modification of anthocyanins was performed in step two; the remaining steps are the same as in Example 1. The cumulative release rate of simulated gastric juice over 1 hour was 23.43%, which is higher than 10%. The cumulative release rate of simulated intestinal juice over 3 hours indicates that untargeted anthocyanins will be rapidly released in gastric juice due to molecular diffusion and then digested and degraded by gastric acid, while the release rate in intestinal juice is reduced due to the lack of targeted modification.

[0055] The enteric-coated capsules obtained in Examples 1 to 7 and Comparative Example 1 were placed in simulated gastric fluid and shaken at 37 ℃ ± 0.5 ℃. The cumulative release rate of proanthocyanidins was then measured at 1 h and 3 h, and the results are shown in Table 1. The enteric-coated capsules obtained in Examples 1 to 7 and Comparative Example 1 were placed in simulated intestinal solution and shaken at 37 ℃ ± 0.5 ℃. The cumulative release rate of proanthocyanidins was then measured at 1 h, 3 h, 6 h and 10 h, and the results are shown in Table 1.

[0056] Table 1. Cumulative release rate of enteric-coated capsules in simulated gastric and intestinal fluids.

[0057] In the above description of exemplary embodiments of the invention, various features of the invention are sometimes grouped together in a single embodiment or in the description thereof. However, this disclosure should not be construed as reflecting an intention that the claimed invention requires more features than are expressly recited in each claim. Rather, as reflected in the claims, the inventive aspect lies in fewer than all the features of the foregoing disclosed embodiments. Therefore, the claims, following the detailed description, are hereby expressly incorporated into that detailed description, wherein each claim itself is a separate embodiment of the invention.

[0058] Although the invention has been described with reference to a limited number of embodiments, those skilled in the art will understand from the foregoing description that other embodiments are conceivable within the scope of the invention described herein. Furthermore, it should be noted that the language used in this specification has been chosen primarily for readability and instructional purposes, and not for the purpose of interpreting or limiting the subject matter of the invention. Therefore, many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the appended claims. The disclosure of the invention is illustrative and not restrictive, and the scope of the invention is defined by the appended claims.

[0059] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A targeted controlled-release enteric-coated capsule for anthocyanins based on purple rice extract, characterized in that, It includes the capsule shell and the contents; the contents contain the following ingredients by weight percentage: 28%-62% targeted modified anthocyanins, 15%-25% purple rice dietary fiber, 10%-20% multivitamin complex, 8%-15% rice milk powder, and 5%-12% protein peptides.

2. The anthocyanin-targeted controlled-release enteric-coated capsule based on purple rice extract according to claim 1, characterized in that, The capsule shell is made of enteric material, which is one or a mixture of several of hydroxypropyl methylcellulose phthalate, cellulose acetate phthalate, polyacrylic acid resin II and III.

3. The anthocyanin-targeted controlled-release enteric-coated capsule based on purple rice extract according to claim 1, characterized in that, Its cumulative anthocyanin release rate in simulated gastric fluid was less than 10% in 1 hour, and its cumulative release rate in simulated intestinal fluid was more than 60% in 3 hours.

4. A method for preparing anthocyanin-targeted controlled-release enteric-coated capsules based on purple rice extract according to any one of claims 1-3, characterized in that, Includes the following steps: Step 1, Preparation of purple rice extract: Take purple rice, pulverize it to 20 mesh, defatt it three times with petroleum ether at 40℃ for 2 hours each time; then add 8 times the volume of 70% ethanol-water solution, extract at 60℃ for 3 hours, filter, concentrate the filtrate to 1 / 5 of the original volume, and then freeze-dry to obtain purple rice extract. Step 2, anthocyanin separation and targeted modification: Anthocyanins were separated from the purple rice extract by high performance liquid chromatography; anthocyanins at a mass ratio of (4-15):1 were reacted with a targeting carrier at 25-35℃ and pH = 6.0-7.0 for 4-8 hours to obtain targeted modified anthocyanins by physical encapsulation. Step 3, Preparation of purple rice dietary fiber: The crude dietary fiber was initially separated from the purple rice extract by centrifugation and filtration, then treated with 0.5% sodium hydroxide solution at 50°C for 2 hours, and then impurities were removed with ion exchange resin to obtain purple rice dietary fiber. Step 4, preparation of the multidimensional complex: Purple rice germ rice is fermented at a temperature of 30-37℃ for 48-72 hours. The fermentation strain is a mixture of lactic acid bacteria and yeast. After fermentation, the multidimensional complex is obtained through filtration, concentration and drying. Step 5, Preparation of protein peptides: Purple rice protein is enzymatically hydrolyzed using various proteases, and the hydrolysis conditions are controlled to obtain protein peptides with an average molecular weight of 800-2000 Da. The amino acid composition meets the requirements, and the content of essential amino acids for the human body is not less than 40%. Step 6, Mixing the contents: Add the targeted modified anthocyanins, purple rice dietary fiber, multi-dimensional complex, rice milk powder, and protein peptides to a three-dimensional mixer according to the mass percentage, and stir at 50 r / min for 30 minutes to obtain a homogeneous mixture of contents; Step 7, Capsule filling: Use a capsule filling machine to fill the above mixture of contents into the capsule shell of the enteric material. Each capsule is filled with 500mg of contents, thus obtaining anthocyanin-targeted controlled-release enteric capsules based on purple rice extract.

5. The preparation method according to claim 4, characterized in that, In step two, the targeting carrier includes one or more combinations of chitosan, sodium alginate, or polylactic-co-glycolic acid copolymer (PLGA).

6. The preparation method according to claim 4, characterized in that, In step three, the dietary fiber content of purple rice is no less than 90%, and the average molecular weight is no less than 5000 Da.

7. The application of anthocyanin-targeted controlled-release enteric-coated capsules based on purple rice extract as described in any one of claims 1-3 in health supplements.

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