A hybridoma cell strain secreting a monoclonal antibody against alginate mannuronate tetrasaccharide epitope, the monoclonal antibody and application

By designing the glycoconjugate KLH-1 to enhance the immunogenicity of mannuron tetrasaccharide, hybridoma cell lines and monoclonal antibodies were obtained, solving the treatment challenges of multidrug resistance and biofilm structure in Pseudomonas aeruginosa, and realizing precise detection and treatment of Pseudomonas aeruginosa.

CN122256267APending Publication Date: 2026-06-23EAST CHINA UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
EAST CHINA UNIV OF SCI & TECH
Filing Date
2026-04-24
Publication Date
2026-06-23

AI Technical Summary

Technical Problem

Multidrug resistance caused by Pseudomonas aeruginosa infection and persistent infection caused by biofilm formation are difficult to treat effectively. The efficacy of existing antibiotics has been greatly weakened, and there is a lack of highly effective immune targets against alginate biofilms.

Method used

The glycoconjugate KLH-1 was designed and synthesized as an immunogen. The immunogenicity of mannuron tetrasaccharide was enhanced by a glycoprotein coupling strategy. Hybridoma cell lines and monoclonal ascites antibodies were obtained using hybridoma technology, which specifically recognize the mannuron tetrasaccharide epitope in alginate.

Benefits of technology

It provides monoclonal antibodies that can specifically identify mucinous and non-mucinous Pseudomonas aeruginosa, for the diagnosis and treatment of Pseudomonas aeruginosa infections, improving infection control levels, and possessing targeted therapy and precise detection capabilities.

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Abstract

The application provides a hybridoma cell strain secreting a monoclonal antibody against a alginate mannuronic acid tetrasaccharide epitope, a monoclonal antibody and application. In order to enhance the immunogenicity of the mannuronic acid tetrasaccharide, a glycoconjugate KLH-1 is used as an immunogen to inject and immunize a mouse, so that the mouse is stimulated to produce a specific immune response against the mannuronic acid tetrasaccharide epitope, then spleen cells of the immunized mouse are fused with myeloma cells, and after screening, an initial hybridoma cell strain is obtained; then after subcloning and specific screening, a hybridoma cell strain capable of stably secreting a monoclonal antibody of the target mannuronic acid tetrasaccharide epitope in alginate is obtained, and a monoclonal ascites antibody is further obtained. The monoclonal ascites antibody shows specific recognition and combination ability for pseudomonas aeruginosa, and the combination activity is related to the expression level of alginate on the surface of the bacteria. Therefore, the monoclonal ascites antibody can be used as a precise detection and diagnosis tool for pseudomonas aeruginosa, and can also be applied to the antibacterial treatment of pseudomonas aeruginosa infection.
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