Method for detecting content of D-choline alfoscerate in L-alpha-choline alfoscerate and application
By combining HPLC-ELSD with a chiral normal-phase column and an evaporative light scattering detector, the problem of detecting D-glucosine isomer impurities in high-purity L-α-glucosine was solved, achieving rapid and accurate quantitative analysis, reducing detection costs and extending column life.
Patent Information
- Application Number
- CN202610710306.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-05-22
- Publication Date
- 2026-06-23
AI Technical Summary
Existing technologies cannot effectively and accurately detect and quantify low levels of D-glucosine isomer impurities in high-purity L-α-glucosine, leading to difficulties in purification and refining processes, as well as high detection costs and long processing times.
The HPLC-ELSD method was used with a cellulose-type chiral normal-phase column covalently bonded to polysaccharides on silica gel or coated with polysaccharides on silica gel, combined with an evaporative light scattering detector. The efficient separation and quantification of D-phosphoric acid choline in L-α-phosphoric acid choline was achieved through isocratic elution and appropriate mobile phase combination.
This method enables rapid and accurate detection of D-glucosamine isomer impurities in L-α-glucosamine choline, reducing detection costs, improving detection sensitivity and reproducibility, and extending the lifespan of chiral chromatographic columns.