A compound strain seed liquid for small peptide cottonseed meal feed solid state fermentation and a method for preparing small peptide cottonseed meal feed by using the seed liquid

By using a ternary complex strain system of Aspergillus niger, Aspergillus oryzae, and Saccharomyces cerevisiae, along with solid-state fermentation technology and additives, the problem of the difficulty in degrading anti-nutritional factors and macromolecular proteins in cottonseed meal feed has been solved, enabling the preparation and industrial application of highly efficient small peptide cottonseed meal feed.

CN122278650APending Publication Date: 2026-06-26ZHENGZHOU UNIV
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Patent Information

Application Number
CN202610620722.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-05-08
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

The application of existing cottonseed meal feed in monogastric animals and aquatic animals is limited, mainly because anti-nutritional factors and large molecular proteins are difficult to degrade effectively, and the efficiency of existing fermentation technology is unstable, making it difficult to achieve industrial production.

Method used

A ternary complex strain system of Aspergillus niger, Aspergillus oryzae, and Saccharomyces cerevisiae was adopted, combined with ammonium chloride and potassium dihydrogen phosphate as additives, and fermentation conditions were optimized to form a stable solid-state fermentation process, which achieved efficient degradation of cottonseed meal protein and removal of anti-nutritional factors.

Benefits of technology

It significantly improves the protein content and mineral utilization of small peptide cottonseed meal feed, degrades anti-nutritional factors, enhances the palatability and digestibility of the feed, completely removes free gossypol from the product, and increases fermentation efficiency by more than 30%.

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Abstract

This invention belongs to the field of feed processing technology and provides a compound bacterial seed liquid for solid-state fermentation of small peptide cottonseed meal feed, and a method for preparing small peptide cottonseed meal feed using this seed liquid. This invention uses a mixture of Aspergillus niger, Aspergillus oryzae, and Saccharomyces cerevisiae seed liquids in a volume ratio of 1:3:1, wherein the viable cell concentrations of Aspergillus niger, Aspergillus oryzae, and Saccharomyces cerevisiae are 1.0×10¹¹ CFU / mL, 1.1×10¹¹ CFU / mL, and 1.2×10¹¹ CFU / mL, respectively. Cottonseed meal is used as the substrate, with the addition of ammonium chloride and potassium dihydrogen phosphate, and fermented under constant temperature. The fermented cottonseed meal prepared by this invention is characterized by high protein, high small peptide content, and zero gossypol, which can significantly improve animal growth performance and provide a feasible solution for the high-value application of cottonseed meal in the feed industry.
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